CN107843595A - Mould test method - Google Patents
Mould test method Download PDFInfo
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- CN107843595A CN107843595A CN201711002316.4A CN201711002316A CN107843595A CN 107843595 A CN107843595 A CN 107843595A CN 201711002316 A CN201711002316 A CN 201711002316A CN 107843595 A CN107843595 A CN 107843595A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56961—Plant cells or fungi
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Abstract
The present invention relates to a kind of mould test method, the mould test method is by applying high imaging photographic system, imaging analysis are carried out to the mildew part of the test specimen before and after mould test, the area ratio that the mould of mould test before and after test sample corrodes is can be derived that, and the kind of grown strain can be drawn by the way that the color and shape for growing strain mycelia is analyzed.Therefore the mould test method can reduce the uncertainty of mould test, the degree of accuracy and the confidence level of mould test are improved, is advantageous to mould test and forms the effective result of the test decision mechanism of a unification.
Description
Technical field
The present invention relates to a kind of mould test method.
Background technology
Mould test is to be used to detect the antimycotic ability of sample and (the i.e. high damp-warm syndrome under conditions of fungus growth is advantageous to
Warm environment is neutralized under the conditions of having inorganic salts existing), if can be by the adverse effect of mould.And existing mould examination at present
Judgement in standard inspection standard to mildew situation is obtained a result simply by visually observing, still, due to experiment in practical operation
The subjective difference of personnel causes the judgement to the mildew situation of sample in mould test to differ, and it is effective can not to form a unification
Result of the test decision mechanism.
Based on the above situation, it is necessary to mesh to mould test result propose one can be with microcosmic visual decision process.
The content of the invention
It is an object of the invention to provide a kind of mould test method, can solve the problem that mould test result of determination can not be microcosmic
The problem of visualization and quantization, so as to improve mould test result, lift the degree of accuracy of mould test.
To reach above-mentioned purpose, the present invention provides following technical scheme：A kind of mould test method, comprises the following steps：
S1, strain and mould test chamber are provided, preliminary treatment is carried out to the strain, and the strain is configured to mix
Spore suspension；The preliminary treatment includes point of strain inspection, culture medium preparation, mycotic spore vitality test and strain
From, transplanting, culture and preservation；
S2, test specimen and control sample are provided, the test specimen is detected to control sample and recorded related
Feature, the correlated characteristic include finish, defect and the material technology of sample.
S3, the test specimen and control sample are pre-processed, by high imaging photographic system to pretreated
The test specimen and control sample carry out shooting archive, to obtain the first test specimen figure and the first control sample figure；
S4, the test specimen and control sample be positioned in the mould test chamber, by the mixing spore suspension
Liquid is sprayed on the test specimen and control sample, shuts the chamber door of the mould test chamber, and starts mould test；
After S5, the mould test terminate, by the high imaging photographic system to the test specimen and control sample
Detected and record related data, meanwhile, observation calculates the area corroded by mould of the test specimen and control sample
Ratio, and archive is shot to obtain the second test specimen figure and the second control sample figure；The related data includes the life of mould
Long position, it is covered with area, color, growth forms, stand density and growth thickness；
S6, by contrasting the first test specimen figure and the second test specimen figure and the first control sample figure and second
Control sample figure carries out the interpretation of result of the mould test.
Further, in step S3, the pretreatment is specially：Using cleaning fluid to the test specimen and control sample
Cleaned, subsequent erasing is simultaneously dried.
Further, the cleaning fluid is selected from 75% alcohol or 95% alcohol or distilled water or the water containing detergent.
Further, the mould test is carried out after the pretreatment terminates 72 hours.
Further, in step S4, the test specimen and control sample are placed on the identical height of the mould test chamber
Degree, and interval between the test specimen and control sample be present.
Further, in step S4, during the mould test, the mould test chamber carried out one every seven days
Secondary ventilation processing.
Further, the mould test method also includes performance judgment, and the performance judgment is specially：
In step S2, the electrical property and mechanical performance of the test specimen are detected；
In step S5, after the mould test terminates, the test specimen is stayed in warm and humid in the mould test chamber
Electrical property and mechanical performance detection are carried out under the conditions of degree；Or at the end of the mould test, taken out of described mould test chamber
Go out the test specimen, and after placing 24 hours under standard atmospheric conditions, carry out electrical property and mechanical performance detection.
Further, the mould test method also includes test reliability judgment step, and the test reliability judges
The step of be specially：
The 7th day carried out in the mould test, checks the control sample from the form of the mould test chamber
Mildew situation, if mould coverage rate is more than or equal to 90%, continue the mould test；If mould coverage rate is less than
90%, then stop and restart the mould test；
The 7th day carried out in the mould test, the strain that culture and preservation were carried out in step S1 is carried out mould
Bacterium spore viability verification, if the growth is normal, continue the mould test；If the strain can not normally give birth to
It is long, then stop the mould test and choose strain again.
Further, the test reliability judges also to include：
In step S5, if the area ratio corroded by mould of the control sample carries out the 7th day less than mould test
The area ratio corroded by mould of the control sample, then stop and restart the mould test.
Further, the high imaging photographic system is microscopic camera.
The beneficial effects of the present invention are：The mould test method of the present invention is by applying high imaging photographic system, to mould
The mildew part of the front and rear test specimen of bacterium experiment carries out imaging analysis, can be derived that the mould of mould test before and after test sample
The area ratio of erosion, and can draw grown bacterium by the way that the color and shape for growing strain mycelia is analyzed
The kind of kind.Therefore the mould test method can reduce the uncertainty of mould test, improve mould test the degree of accuracy and can
Reliability, be advantageous to mould test and form the effective result of the test decision mechanism of a unification.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention,
And can be practiced according to the content of specification, below with presently preferred embodiments of the present invention and coordinate accompanying drawing describe in detail as after.
Brief description of the drawings
Fig. 1 is the flow chart of the mould test method shown in one embodiment of the invention；
Fig. 2 is the microphotogram of the test specimen shown in one embodiment of the invention.
Embodiment
With reference to the accompanying drawings and examples, the embodiment of the present invention is described in further detail.Implement below
Example is used to illustrate the present invention, but is not limited to the scope of the present invention.
Fig. 1 is referred to, mould test method of the invention comprises the following steps：
S1, strain and mould test chamber are provided, preliminary treatment is carried out to the strain, and the strain is configured to mix
Spore suspension；The preliminary treatment includes point of strain inspection, culture medium preparation, mycotic spore vitality test and strain
From, transplanting, culture and preservation；
S2, test specimen and control sample are provided, the test specimen is detected to control sample and recorded related
Feature, the correlated characteristic include finish, defect and the material technology of sample；
S3, the test specimen and control sample are pre-processed, i.e., using cleaning fluid to the test specimen and right
Product are cleaned in the same old way, and subsequent erasing is simultaneously dried, then by high imaging photographic system to the pretreated test specimen
Shooting archive is carried out with control sample, to obtain the first test specimen figure and the first control sample figure；
S4, after the pretreatment terminates 72 hours, the test specimen and control sample are positioned over the mould and tried
In tryoff, in order to ensure the degree of accuracy of mould test, the test specimen and control sample are placed on the mould test chamber
Identical height, and interval between the test specimen and control sample be present；Then the mixing spore suspension is sprayed to
On the test specimen and control sample, the chamber door of the mould test chamber is shut, and starts mould test, is tried in the mould
During testing, the mould test chamber carried out once ventilation every seven days and handled；
After S5, the mould test terminate, by the high imaging photographic system to the test specimen and control sample
Detected and record related data, meanwhile, observation calculates the area corroded by mould of the test specimen and control sample
Ratio, and archive is shot to obtain the second test specimen figure and the second control sample figure；The related data includes the life of mould
Long position, it is covered with area, color, growth forms, stand density and growth thickness；
S6, by contrasting the first test specimen figure and the second test specimen figure and the first control sample figure and second
Control sample figure carries out the interpretation of result of the mould test, i.e. the mildew part of the test specimen before and after mould test is entered
Row imaging analysis, the area ratio that the mould of mould test before and after test sample corrodes is can be derived that, and by growing strain
The kind that can draw grown strain is analyzed in the color and shape of mycelia.
In order to ensure the degree of accuracy of mould test method, mould test method of the invention also includes test reliability and judged
Step, the step of test reliability judges be specially：
The 7th day carried out in the mould test, checks the control sample from the form of the mould test chamber
Mildew situation, if mould coverage rate is more than or equal to 90%, continue the mould test；If mould coverage rate is less than
90%, then stop and restart the mould test；
The 7th day carried out in the mould test, the strain that culture and preservation were carried out in step S1 is carried out mould
Bacterium spore viability verification, if the growth is normal, continue the mould test；If the strain can not normally give birth to
It is long, then stop the mould test and choose strain again.
In step s 5, if the area ratio corroded by mould of the control sample is carried out the 7th day less than mould test
The area ratio corroded by mould of Shi Suoshu control samples, then stop and restart the mould test.
In the present embodiment, the mould test method also includes performance judgment：
In step S2, the electrical property and mechanical performance of the test specimen are detected；
In step S5, after the mould test terminates, the test specimen is stayed in warm and humid in the mould test chamber
Electrical property and mechanical performance detection are carried out under the conditions of degree；Or at the end of the mould test, taken out of described mould test chamber
Go out the test specimen, and after placing 24 hours under standard atmospheric conditions, carry out electrical property and mechanical performance detection.
Really, in other embodiments, the detection of the electrical property of test specimen and mechanical performance can be selected according to actual conditions
Selecting property is carried out.
In the present embodiment, in step S3, the cleaning fluid is 75% alcohol, really, in other embodiments, the cleaning
Liquid can also be 95% alcohol or distilled water or the water containing detergent.In the present embodiment, the high imaging photographic system is micro-
Camera, really, in other embodiments, the high imaging photographic system can also be selected according to being actually needed.
Fig. 2 is referred to, as seen from the figure, test specimen in the present embodiment is after the mould test, sample surfaces
Growth has obvious mycelia and spore, but its matrix is not corroded significantly but.However, in excessively existing mould test,
Explanation of taking pictures only generally is carried out to the overall picture of test specimen, whether has corrosion for the matrix in standard determination, by visual observation with
The shooting of general camera can not determine.Also work is supported simultaneously for the whether good upgrowth situation of mould without the photo of correlation
For evidence, and when directly shooting close-up view, the uniformity due to spraying spore suspension is different and may lead to not determine
The specific growing state of mould.
In summary：The mould test method of the present invention is by applying high imaging photographic system, before and after mould test
The mildew part of test specimen carries out imaging analysis, can be derived that the area ratio that the mould of mould test before and after test sample corrodes
Example, and the kind of grown strain can be drawn by the way that the color and shape for growing strain mycelia is analyzed.Therefore
The mould test method can reduce the uncertainty of mould test, improve the degree of accuracy and the confidence level of mould test, be advantageous to
Mould test forms the effective result of the test decision mechanism of a unification.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope that this specification is recorded all is considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that come for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
- A kind of 1. mould test method, it is characterised in that comprise the following steps：S1, strain and mould test chamber are provided, preliminary treatment is carried out to the strain, and the strain is configured to mixing spore Suspension；The preliminary treatment includes strain inspection, culture medium preparation, mycotic spore vitality test and the separation of strain, moved Plant, culture and preservation；S2, test specimen and control sample are provided, the test specimen and control sample are detected and record correlated characteristic, The correlated characteristic includes finish, defect and the material technology of sample.S3, the test specimen and control sample are pre-processed, by high imaging photographic system to pretreated described Test specimen and control sample carry out shooting archive, to obtain the first test specimen figure and the first control sample figure；S4, the test specimen and control sample be positioned in the mould test chamber, by the mixing spore suspension spray Spill to the test specimen and control sample, shut the chamber door of the mould test chamber, and start mould test；After S5, the mould test terminate, the test specimen and control sample are carried out by the high imaging photographic system Detect and record related data, meanwhile, observation calculates the area ratio corroded by mould of the test specimen and control sample, And archive is shot to obtain the second test specimen figure and the second control sample figure；The related data includes the growing part of mould Position, it is covered with area, color, growth forms, stand density and growth thickness；S6, compareed with the second test specimen figure and the first control sample figure with second by contrasting the first test specimen figure Sample drawing carries out the interpretation of result of the mould test.
- 2. mould test method as claimed in claim 1, it is characterised in that in step S3, the pretreatment is specially：Use Cleaning fluid is cleaned to the test specimen and control sample, and subsequent erasing is simultaneously dried.
- 3. mould test method as claimed in claim 2, it is characterised in that the cleaning fluid is selected from 75% alcohol or 95% wine Essence or distilled water or the water containing detergent.
- 4. mould test method as claimed in claim 1, it is characterised in that the mould test terminates 72 in the pretreatment Carried out after hour.
- 5. mould test method as claimed in claim 4, it is characterised in that in step S4, the test specimen and control sample Product are placed on the identical height of the mould test chamber, and interval between the test specimen and control sample be present.
- 6. mould test method as claimed in claim 5, it is characterised in that in step S4, in the process of the mould test In, the mould test chamber carried out once ventilation every seven days and handled.
- 7. mould test method as claimed in claim 1, it is characterised in that the mould test method is also sentenced including performance Fixed, the performance judgment is specially：In step S2, the electrical property and mechanical performance of the test specimen are detected；In step S5, after the mould test terminates, humiture bar that the test specimen is stayed in the mould test chamber Electrical property and mechanical performance detection are carried out under part；Or at the end of the mould test, institute is taken out out of described mould test chamber State test specimen, and after placing 24 hours under standard atmospheric conditions, carry out electrical property and mechanical performance detection.
- 8. mould test method as claimed in claim 1, it is characterised in that it is reliable that the mould test method also includes experiment Property judgment step, the step of test reliability judges be specially：The 7th day carried out in the mould test, the mildew of the control sample is checked from the form of the mould test chamber Situation, if mould coverage rate is more than or equal to 90%, continue the mould test；If mould coverage rate is less than 90%, Stop and restart the mould test；The 7th day carried out in the mould test, mould spore is carried out to the strain that culture and preservation were carried out in step S1 Sub- vitality test, if the growth is normal, continue the mould test；If the strain can not normal growth, Then stop the mould test and choose strain again.
- 9. mould test method as claimed in claim 8, it is characterised in that the test reliability judges also to include：In step S5, if described in when the area ratio corroded by mould of the control sample is carried out the 7th day less than mould test The area ratio corroded by mould of control sample, then stop and restart the mould test.
- 10. mould test method as claimed in any one of claims 1-9 wherein, it is characterised in that the high imaging photographic system For microscopic camera.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711002316.4A CN107843595B (en) | 2017-10-24 | 2017-10-24 | Mould test method |
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| CN201711002316.4A CN107843595B (en) | 2017-10-24 | 2017-10-24 | Mould test method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110196221A (en) * | 2019-05-07 | 2019-09-03 | 青岛苏试海测检测技术有限公司 | One kind being used for the resistance to microorganism adaptive detection method of spinning coating |
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2017
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|---|---|---|---|---|
| JPH0795896A (en) * | 1993-09-29 | 1995-04-11 | Sekisui Chem Co Ltd | Method for testing antifungal performance |
| CN101497916A (en) * | 2009-01-23 | 2009-08-05 | 东华大学 | Method for detecting mould fungus inhibition effect of leather material and product thereof |
| CN106047983A (en) * | 2016-06-14 | 2016-10-26 | 苏州市职业大学 | Method for detecting anti-mold performance of silkworm excrement product |
| CN106119337A (en) * | 2016-06-27 | 2016-11-16 | 山东省农业科学院生物技术研究中心 | A kind of Rapid identification peanut varieties method to Aspergillus flavus resistance |
| CN206573589U (en) * | 2017-03-16 | 2017-10-20 | 江苏农林职业技术学院 | Crop fungal spore liquefaction resistance device |
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| Title |
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| SIMONA STRNAD ET AL.: "Antifungal Activity Assessment of Cotton Fabrics Using Image Processing and Analysis", 《FIBRES & TEXTILES IN EASTERN EUROPE》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110196221A (en) * | 2019-05-07 | 2019-09-03 | 青岛苏试海测检测技术有限公司 | One kind being used for the resistance to microorganism adaptive detection method of spinning coating |
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| CN107843595B (en) | 2020-06-26 |
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