CN107836662A - A kind of preparation method for being used to treat the yolk powder of hypertension - Google Patents
A kind of preparation method for being used to treat the yolk powder of hypertension Download PDFInfo
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- CN107836662A CN107836662A CN201711356183.0A CN201711356183A CN107836662A CN 107836662 A CN107836662 A CN 107836662A CN 201711356183 A CN201711356183 A CN 201711356183A CN 107836662 A CN107836662 A CN 107836662A
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- Prior art keywords
- take
- add
- yolk
- yolk powder
- parts
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- 239000000843 powder Substances 0.000 title claims abstract description 70
- 206010020772 Hypertension Diseases 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 239000000696 magnetic material Substances 0.000 claims abstract description 24
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 21
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 21
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- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims abstract description 8
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- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 claims description 12
- 240000005250 Chrysanthemum indicum Species 0.000 claims description 12
- 235000018959 Chrysanthemum indicum Nutrition 0.000 claims description 12
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 12
- 235000009388 Parthenocissus quinquefolia Nutrition 0.000 claims description 12
- 241000219099 Parthenocissus quinquefolia Species 0.000 claims description 12
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- KSBAEPSJVUENNK-UHFFFAOYSA-L tin(ii) 2-ethylhexanoate Chemical compound [Sn+2].CCCCC(CC)C([O-])=O.CCCCC(CC)C([O-])=O KSBAEPSJVUENNK-UHFFFAOYSA-L 0.000 claims description 12
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- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 6
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- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 6
- 101000905241 Mus musculus Heart- and neural crest derivatives-expressed protein 1 Proteins 0.000 claims description 6
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- 239000002054 inoculum Substances 0.000 claims description 6
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 claims description 6
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 6
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 6
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 6
- 229920001277 pectin Polymers 0.000 claims description 6
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- 229910001873 dinitrogen Inorganic materials 0.000 claims description 4
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 claims description 4
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 24
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- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 4
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 3
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L15/00—Egg products; Preparation or treatment thereof
- A23L15/25—Addition or treatment with microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/273—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption using adsorption or absorption agents, resins, synthetic polymers, or ion exchangers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/287—Chrysanthemum, e.g. daisy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Mycology (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to hypertension practical technique field, is specifically related to a kind of preparation method for being used to treat the yolk powder of hypertension.The present invention adds crosslinking agent and catalyst, acted on by the connection of center casing glycan and beta cyclodextrin is fixed on magnetic nanoparticle surface, it can disperse in normal temperature system, quick separating and with preferable reusing magnetic material, to remove cholesterol in yolk, the NH in chitosan molecule2With NH under low in acidity environment3+Ionic species is present, and when magnetic material acts on yolk system, and yolk protein and cholesterol molecule can simultaneously and NH3+Acted in the form of hydrogen bond, Competition between albumen and cholesterol molecule be present, this Competition weakens in magnetic material chitosan molecule to the noncovalent interaction of cholesterol, improve suction-operated, Cholesterol removal efficiency is improved, reduce cholesterol in yolk powder and advantageously reduce blood pressure blood fat, and nutritional ingredient is not lost in, and has reached functional health effect.
Description
Technical field
The present invention relates to hypertension practical technique field, is specifically related to a kind of system for being used to treat the yolk powder of hypertension
Preparation Method.
Background technology
Hypertension is one of commonly encountered diseases of internal department, be with arterial pressure raise based on clinical syndrome.With human lives
The change of mode and living environment, the ratio more and more higher that hypertension is accounted in total population, Chinese Hypertensive Population already exceed 1.6
Hundred million.Authority counts more than half hyperpietic and dies from kidney failure, heart failure, is counted for much with western medicine of depressurization.Western medicine can make trouble
Person produces strong drug dependence.Hypotensive on surface, the heart, brain, kidney are wrecked actually, but also can bring dizzy, nausea, the heart
Unbearably, the side effect such as heavy breathing and oedema, huge pain is brought to patient.For those sleeps, caused false high blood of feeling bad
Pressure has sentenced death penalty substantially once depressor is taken.Depressor promotes the body blood pressure regulating mechanisms of patient to tell degeneration, high blood
Pressure wants the function of requesting medicine to recover human blood-pressure regulating system, it is impossible to and blindness is depressured using Western medicine, because
Allow you to be unable to do without it forever once taking, therefore can develop with treatment high blood pressure disease, reduce heart and brain to greatest extent
Incidence and the death rate beyond blood vessel, and extend the Chinese medicine of patient vitals with regard to significant.
Yolk powder contains animal protein needed by human, fat, lecithin and minerals and vitamins, its component
Quality is high, and absorptivity is high, and particularly human brain and nervous system is indispensable, and its content of phospholipid is relatively abundanter.Yolk powder is main
Phosphatide containing 30% or so protein and 38% or so glyceride and 19% or so, also a small amount of carbohydrate, mineral
Matter, vitamin, pigment, enzyme etc..Yolk powder is a kind of very high food of nutritive value, while is widely used as food ingredient
In the baked goods such as bread, cake and ice cream, tartar sauce, nutritional formulas.Yolk powder is mainly used in food industry
Raw material and pharmaceutical sector extract lecithin, in the food industry bakery, cold drink（Ice cream, ice cream）And full nutrition formula food
Product etc..The emulsibility of yolk powder is mainly lipoprotein（Especially account for the low-density lipoprotein of gross protein 65%）And its component
Lecithin（Account for the 75% of phosphatide）Effect, contribution of the protein to emulsification be bigger than phosphatide.
The yolk powder functional character of existing process technology production is poor, and this significantly impacts the application of yolk powder, also limits
The development of yolk powder industry is made, so need to be modified to yolk.The emulsibility of yolk is mainly by lipoprotein and lecithin
Contribution, but the natural structure of phosphatide is then destroyed using chemical modification to some extent, and chemical group is introduced, peace be present
Full sex chromosome mosaicism, some national food additives standards are not met.In addition, existing yolk Powder Particle Size is generally 60~100 mesh,
Solution system stability is poor existing for yolk powder, and particle is uneven, and easily bonding etc. reconstitutes sex chromosome mosaicism.In cold water, warm water, hot water
It is dispersed and scattered after heat resistance（Dispersiveness is destroyed after heated, it is impossible to and it is fully dispersed, form flocculate --- egg
Flower）, this is totally unfavorable for production cold drink and preparation nutraceutical, and the characteristic and baking system of dough are also influenceed in bakery
Product color and luster, that is, the application of yolk powder is have impact on, also limit the development of yolk powder industry, it is due to reduce yolk powder
Market.
In summary, nowadays the yolk powder of in the market is primarily present the defects of following：Existing yolk Powder Particle Size one
As be 60~100 mesh, solution system stability is poor existing for yolk powder, after dispersed and scattered in cold water, warm water, hot water
Poor heat resistance, particle is uneven, easily bond.
Therefore, if can develop it is a kind of conveniently, safely, nutrition be used for treat the yolk powder of hypertension, both meet city
The demand of field, and a kind of embodiment of CSR.
The content of the invention
The technical problems to be solved by the invention：It is poor for solution system stability existing for current yolk powder, cold water,
Poor heat resistance after dispersed and scattered in warm water, hot water, the unconspicuous problem of blood pressure lowering effect, there is provided one kind is used to treat
The preparation method of the yolk powder of hypertension.
In order to solve the above technical problems, the present invention is using technical scheme as described below：
A kind of preparation method for being used to treat the yolk powder of hypertension, the preparation method comprise the following steps：
（1）Take egg yolk in mass ratio 1：6 add absolute ethyl alcohol mixing, extraction, take extract to centrifuge, and take precipitation rotation to steam
Hair, freeze-drying, obtains degreasing thing, extracting degreasing thing 1：5 add distilled water mixing, add alkali protease and phospholipase A2, enzyme
Solution, obtains enzymolysis liquid, takes enzymolysis liquid in mass ratio 5：2 add in 0.1mol/L sodium hydroxide solution, are stirred, obtain yolk powder
Matrix, it is standby；
（2）Take ferrous sulfate, iron chloride in mass ratio 1：3：12 ~ 15, which add the ethanol solution that mass fraction is 70%, mixes, and is passed through
Nitrogen, stirring, the ammoniacal liquor for the ethanol solution quality 40 ~ 50% that mass fraction is 70% is added, keeps 20 ~ 30 min, magnet is inhaled
Draw, collect magnetic-particle A, washed respectively with ethanol and deionized water, be freeze-dried, obtain magnetic matrix thing；
（3）Take chitosan in mass ratio 1：50, which add the acetic acid that mass fraction is 2%, mixes, and obtains mixed solution, mixed solution is pressed
Mass ratio 10：7 add in magnetic matrix thing, are stirred, and add 4,4'- dicyclohexyl methyl hydride diisocyanates and octanoic acid is sub-
Tin, it is stirred, filters, take filter residue, take beta-schardinger dextrin powder in mass ratio 1：10 add dimethyl sulfoxide mixing, must mix molten
Liquid, take filter residue in mass ratio 20：30：1 adds mixed solution and stannous octoate, reaction, attraction, collects magnetic-particle B, uses
Absolute ethyl alcohol washs, and freeze-drying, obtains Magnetic Materials；
（4）Take step（1）Standby yolk powder matrix thing in mass ratio 1：10 add deionized water mixing, adjust pH to 6 ~ 6.5,
The Magnetic Materials of deionized water quality 10 ~ 12% are added, 2 ~ 3h is kept in 25 ~ 30 DEG C, centrifugation, supernatant is taken, attraction, shakes
Swing, reclaim Magnetic Materials, egg yolk liquid after must handling；
（5）According to the mass fraction, take 100 ~ 150 parts processing after egg yolk liquid, 4 ~ 5 portions of maltose, 5 ~ 6 parts of pectin, 70 ~ 90 parts of water,
20 ~ 30 parts of aid nutrition additives, are stirred, and spray drying, obtain the yolk powder for treating hypertension.
The step（1）Neutral and alkali protease and phospholipase A2Addition be fat-free mass amount 6 ~ 7% alkali protease
With the phospholipase A of fat-free mass amount 2 ~ 3%2；Enzymatic hydrolysis condition is：2 ~ 3h is digested in 50 ~ 55 DEG C.
The step（3）The addition of middle 4,4'- dicyclohexyl methyl hydride diisocyanates and stannous octoate is：Respectively magnetic
Property base matter amount 7 ~ 9% and 0.2 ~ 0.5%.
The step（5）Middle health-care assistance additive：The root of kudzu vine, ampelopsis grossdentata leaf, chrysanthemum indicum is taken to clean, by mass fraction
Meter, takes 6 ~ 10 parts of the root of kudzu vine, 4 ~ 6 parts, 12 ~ 15 parts chrysanthemum indicums of ampelopsis grossdentata leaf, 20 ~ 25 parts of sterilized waters, is homogenized, obtains homogenate,
Take Lactobacillus plantarum to be seeded to by 4% inoculum concentration in homogenate, fermented 1 ~ 2 day in 38 ~ 42 DEG C, obtain zymotic fluid, produce auxiliary and protect
Strong additive.
Compared with other method, advantageous effects are the present invention：
（1）The present invention utilizes phospholipase A2Egg yolk liquid is pre-processed with alkali protease, phospholipase A2It is selectivity catalysis
The acyl hydrolase generation lysophosphatide of the Sn-2 positions of phosphatide and the enzyme of aliphatic acid, make α or β positions lose aliphatic acid, what is obtained is molten
Blood phospholipid, lysophosphatide are molten so as to greatly enhance hydrophilicity due to hydrolyzing the phthalidyl of position so that non-polar group is reduced
Blood phospholipid has more preferable water dispersible than common phosphatide, and its emulsifiability is high, and under high temperature, low value, high ionic strength environment
With good emulsion stability, improve solution system stability existing for yolk powder, point in cold water, warm water, hot water
Dissipate property and heat resistance improves, on the other hand add alkali protease, be advantageous to using alkali protease under basic hydrolysis environment
The dephosphorylation of albumen, mild condition, low-phosphorousization albumen powder help to reduce the burden to kidney, can prevent kidney failure and control
Nephrosis is treated, while hydrolyzes yolk powder can effectively suppress osteoclast differentiation and absorb, promotes Oesteoblast growth, there is provided
The nutritious supplementary pharmaceutical of low-phosphorous high calcium；
（2）The present invention adds crosslinking agent and catalyst, is acted on by the connection of center casing glycan beta-schardinger dextrin being fixed on magnetic
Property nano grain surface, it can disperse in normal temperature system, quick separating and with preferable reusing magnetic material, with
Cholesterol in yolk is removed ,-the NH in chitosan molecule2With NH under low in acidity environment3+Ionic species is present, and magnetic material
When acting on yolk system, yolk protein and cholesterol molecule can simultaneously and NH3+Acted in the form of hydrogen bond, albumen and cholesterol
Intermolecular have a Competition, and this Competition weakens in magnetic material chitosan molecule to the non-covalent work of cholesterol
With, improve suction-operated, Cholesterol removal efficiency is improved, reduce yolk powder in cholesterol advantageously reduce blood pressure blood
Fat, and nutritional ingredient is not lost in, and has reached functional health effect；
（3）Auxiliary type hypotensive additive is added, is prepared using fermentation process, wherein polysaccharide constituents may act as the angle of prebiotics
Color, these prebioticses can be used as substrate to utilize or change the effect such as nutritional ingredient, growth conditions by probiotics, promote enteron aisle
The growth of probiotics, so as to promote the raising of nutriment activity in yolk powder, absorbed so as to be digested, improve hypotensive
The drug effect of active ingredient plays, so as to reach the effect of hypotensive.
Embodiment
A kind of preparation method for being used to treat the yolk powder of hypertension, comprises the following steps：
Health-care assistance additive：Take the root of kudzu vine, ampelopsis grossdentata leaf, chrysanthemum indicum to clean, according to the mass fraction, take 6 ~ 10 parts of the root of kudzu vine,
4 ~ 6 parts, 12 ~ 15 parts chrysanthemum indicums of ampelopsis grossdentata leaf, 20 ~ 25 parts of sterilized waters, homogenate, obtain homogenate, take Lactobacillus plantarum by 4%
Inoculum concentration be seeded in homogenate, in 38 ~ 42 DEG C ferment 1 ~ 2 day, obtain zymotic fluid, produce health-care assistance additive.
（1）Take egg yolk in mass ratio 1：6 add absolute ethyl alcohols mixing, in 25 ~ 30 DEG C extract 1 ~ 1.5h, take extract in
10000 r/min, 4 ~ 5 DEG C, 20 min of centrifugation, take precipitation rotary evaporation, are freeze-dried, obtain degreasing thing, extracting degreasing thing 1：5 add
Distilled water mixes, and adds the alkali protease of fat-free mass amount 6 ~ 7% and the phospholipase A of fat-free mass amount 2 ~ 3%2, in 50 ~ 55
DEG C enzymolysis 2 ~ 3h, obtain enzymolysis liquid, take enzymolysis liquid in mass ratio 5：2 add in 0.1mol/L sodium hydroxide solution, are stirred 1
~ 1.5h, yolk powder matrix is obtained, it is standby；
（2）Take ferrous sulfate, iron chloride in mass ratio 1：3：12 ~ 15, which add the ethanol solution that mass fraction is 70%, mixes, and is passed through
Nitrogen, 20 ~ 30 min are stirred in 55 ~ 60 DEG C, add the ammonia for the ethanol solution quality 40 ~ 50% that mass fraction is 70%
Water, 20 ~ 30 min are kept, attraction, magnetic-particle A is collected, is washed 2 ~ 3 times with ethanol and deionized water respectively, freezing is dry
It is dry, obtain magnetic matrix thing；
（3）Take chitosan in mass ratio 1：50, which add the acetic acid that mass fraction is 2%, mixes, and mixed solution is obtained, at 55 ~ 60 DEG C
By mixed solution in mass ratio 10：7 add in magnetic matrix thing, and 600 r/min stir 1 ~ 1.5h, add magnetic matrix amount of substance
7 ~ 9% 4,4'- dicyclohexyl methyl hydride diisocyanates and the stannous octoate of magnetic matrix amount of substance 0.2 ~ 0.5%, are stirred 2
~ 3h, filtering, takes filter residue, takes beta-schardinger dextrin powder in mass ratio 1：10 add dimethyl sulfoxide mixing, obtain mixed solution, take filter residue
In mass ratio 20：30：1 adds mixed solution and stannous octoate, and 1 ~ 2 h is reacted in 20 ~ 25 DEG C, attraction, collects magnetic
Grain B, is washed with absolute ethyl alcohol, is freeze-dried, is obtained Magnetic Materials；
（4）Take step（1）Standby yolk powder matrix thing in mass ratio 1：10 add deionized water mixing, adjust pH to 6 ~ 6.5,
The Magnetic Materials of deionized water quality 10 ~ 12% are added, 2 ~ 3h is kept in 25 ~ 30 DEG C, centrifugation, supernatant is taken, attraction, shakes
30 ~ 60min is swung, reclaims Magnetic Materials, egg yolk liquid after must handling；
（5）According to the mass fraction, take 100 ~ 150 parts processing after egg yolk liquid, 4 ~ 5 portions of maltose, 5 ~ 6 parts of pectin, 70 ~ 90 parts of water,
20 ~ 30 parts of aid nutrition additives, 30 ~ 40min is stirred in 30 ~ 40 DEG C, is spray-dried, obtains the egg for treating hypertension
Bloom.
Embodiment 1
A kind of preparation method for being used to treat the yolk powder of hypertension, comprises the following steps：
Health-care assistance additive：Take the root of kudzu vine, ampelopsis grossdentata leaf, chrysanthemum indicum to clean, according to the mass fraction, take 6 parts of the root of kudzu vine, aobvious tooth
4 parts, 12 parts chrysanthemum indicums of porcelain ampelopsis leaf, 20 parts of sterilized waters, homogenate, obtain homogenate, take Lactobacillus plantarum to be inoculated with by 4% inoculum concentration
Into homogenate, fermented 1 day in 38 DEG C, obtain zymotic fluid, produce health-care assistance additive.
（1）Take egg yolk in mass ratio 1：6 add absolute ethyl alcohol mixing, extract 1h in 25 DEG C, take extract in 10000 r/
Min, 4 DEG C, 20 min of centrifugation, take precipitation rotary evaporation, are freeze-dried, obtain degreasing thing, extracting degreasing thing 1：5 addition distilled water mix
Close, add the alkali protease of fat-free mass amount 6 ~ 7% and the phospholipase A of fat-free mass amount 2 ~ 3%2, 2h is digested in 50 DEG C, is obtained
Enzymolysis liquid, take enzymolysis liquid in mass ratio 5：2 add in 0.1mol/L sodium hydroxide solution, are stirred 1h, obtain yolk powder base
Body, it is standby；
（2）Take ferrous sulfate, iron chloride in mass ratio 1：3：12, which add the ethanol solution that mass fraction is 70%, mixes, and is passed through nitrogen
Gas, 20 min are stirred in 55 DEG C, add the ammoniacal liquor for the ethanol solution quality 40% that mass fraction is 70%, keep 20min,
Attraction, magnetic-particle A is collected, is washed 2 times with ethanol and deionized water respectively, be freeze-dried, obtain magnetic matrix thing；
（3）Take chitosan in mass ratio 1：50, which add the acetic acid that mass fraction is 2%, mixes, and obtains mixed solution, will be mixed at 55 DEG C
Close solution in mass ratio 10：7 add in magnetic matrix thing, 600 r/min stirring 1h, add the 4 of magnetic matrix amount of substance 7%,
The stannous octoate of 4'- dicyclohexyl methyl hydride diisocyanates and magnetic matrix amount of substance 0.2%, 2h is stirred, filters, take filter
Slag, take beta-schardinger dextrin powder in mass ratio 1：10 add dimethyl sulfoxide mixing, obtain mixed solution, take filter residue in mass ratio 20：30：
1 adds mixed solution and stannous octoate, and 1 h is reacted in 20 DEG C, attraction, magnetic-particle B is collected, is washed with absolute ethyl alcohol,
Freeze-drying, obtains Magnetic Materials；
（4）Take step（1）Standby yolk powder matrix thing in mass ratio 1：10 add deionized water mixing, and pH is to 6 for regulation, then adds
Enter the Magnetic Materials of deionized water quality 10%, 2h is kept in 25 DEG C, centrifugation, supernatant is taken, attraction, vibrates 30min, recovery
Magnetic Materials, egg yolk liquid after must handling；
（5）According to the mass fraction, take 100 parts processing after egg yolk liquid, 4 portions of maltose, 5 parts of pectin, 70 parts of water, 20 parts of aid nutritions
Additive, 30min is stirred in 30 DEG C, is spray-dried, obtains the yolk powder for treating hypertension.
Embodiment 2
A kind of preparation method for being used to treat the yolk powder of hypertension, comprises the following steps：
Health-care assistance additive：Take the root of kudzu vine, ampelopsis grossdentata leaf, chrysanthemum indicum to clean, according to the mass fraction, take 7 parts of the root of kudzu vine, aobvious tooth
5 parts, 13 parts chrysanthemum indicums of porcelain ampelopsis leaf, 22 parts of sterilized waters, homogenate, obtain homogenate, take Lactobacillus plantarum to be inoculated with by 4% inoculum concentration
Into homogenate, fermented 1 day in 40 DEG C, obtain zymotic fluid, produce health-care assistance additive.
（1）Take egg yolk in mass ratio 1：6 add absolute ethyl alcohol mixing, extract 1.2h in 27 DEG C, take extract in 10000
R/min, 4 DEG C, 20 min of centrifugation, take precipitation rotary evaporation, are freeze-dried, obtain degreasing thing, extracting degreasing thing 1：5 addition distilled water mix
Close, add the alkali protease of fat-free mass amount 6% and the phospholipase A of fat-free mass amount 2%2, 2h is digested in 52 DEG C, must be digested
Liquid, take enzymolysis liquid in mass ratio 5：2 add in 0.1mol/L sodium hydroxide solution, are stirred 1.5h, obtain yolk powder matrix,
It is standby；
（2）Take ferrous sulfate, iron chloride in mass ratio 1：3：13, which add the ethanol solution that mass fraction is 70%, mixes, and is passed through nitrogen
Gas, 25 min are stirred in 57 DEG C, add the ammoniacal liquor for the ethanol solution quality 45% that mass fraction is 70%, keep 25
Min, attraction, magnetic-particle A is collected, is washed 2 times with ethanol and deionized water respectively, be freeze-dried, obtain magnetic matrix thing；
（3）Take chitosan in mass ratio 1：50, which add the acetic acid that mass fraction is 2%, mixes, and obtains mixed solution, will be mixed at 57 DEG C
Close solution in mass ratio 10：7 add in magnetic matrix thing, 600 r/min stirring 1.2h, add the 4 of magnetic matrix amount of substance 8%,
The stannous octoate of 4'- dicyclohexyl methyl hydride diisocyanates and magnetic matrix amount of substance 0.3%, 2h is stirred, filters, take filter
Slag, take beta-schardinger dextrin powder in mass ratio 1：10 add dimethyl sulfoxide mixing, obtain mixed solution, take filter residue in mass ratio 20：30：
1 adds mixed solution and stannous octoate, and 1h is reacted in 23 DEG C, attraction, magnetic-particle B is collected, is washed with absolute ethyl alcohol, cold
It is lyophilized dry, obtain Magnetic Materials；
（4）Take step（1）Standby yolk powder matrix thing in mass ratio 1：10 add deionized water mixing, and pH is to 6.2 for regulation, then
The Magnetic Materials of deionized water quality 11% are added, 2h is kept in 27 DEG C, centrifugation, supernatant is taken, attraction, vibrates 40min, return
Receive Magnetic Materials, egg yolk liquid after must handling；
（5）According to the mass fraction, take 120 parts processing after egg yolk liquid, 5 portions of maltose, 6 parts of pectin, 80 parts of water, 25 parts of aid nutritions
Additive, 35min is stirred in 35 DEG C, is spray-dried, obtains the yolk powder for treating hypertension.
Embodiment 3
A kind of preparation method for being used to treat the yolk powder of hypertension, comprises the following steps：
Health-care assistance additive：Take the root of kudzu vine, ampelopsis grossdentata leaf, chrysanthemum indicum to clean, according to the mass fraction, take 10 parts of the root of kudzu vine, show
6 parts, 15 parts chrysanthemum indicums of tooth porcelain ampelopsis leaf, 25 parts of sterilized waters, homogenate, obtain homogenate, take Lactobacillus plantarum to be connect by 4% inoculum concentration
Kind is fermented 2 days in 42 DEG C into homogenate, obtains zymotic fluid, produce health-care assistance additive.
（1）Take egg yolk in mass ratio 1：6 add absolute ethyl alcohol mixing, extract 1.5h in 30 DEG C, take extract in 10000
R/min, 5 DEG C, 20 min of centrifugation, take precipitation rotary evaporation, are freeze-dried, obtain degreasing thing, extracting degreasing thing 1：5 addition distilled water mix
Close, add the alkali protease of fat-free mass amount 7% and the phospholipase A of fat-free mass amount 3%2, 3h is digested in 55 DEG C, must be digested
Liquid, take enzymolysis liquid in mass ratio 5：2 add in 0.1mol/L sodium hydroxide solution, are stirred 1.5h, obtain yolk powder matrix,
It is standby；
（2）Take ferrous sulfate, iron chloride in mass ratio 1：3：15, which add the ethanol solution that mass fraction is 70%, mixes, and is passed through nitrogen
Gas, 30 min are stirred in 60 DEG C, add the ammoniacal liquor for the ethanol solution quality 50% that mass fraction is 70%, keep 30
Min, attraction, magnetic-particle A is collected, is washed 3 times with ethanol and deionized water respectively, be freeze-dried, obtain magnetic matrix thing；
（3）Take chitosan in mass ratio 1：50, which add the acetic acid that mass fraction is 2%, mixes, and obtains mixed solution, will be mixed at 60 DEG C
Close solution in mass ratio 10：7 add in magnetic matrix thing, 600 r/min stirring 1.5h, add the 4 of magnetic matrix amount of substance 9%,
The stannous octoate of 4'- dicyclohexyl methyl hydride diisocyanates and magnetic matrix amount of substance 0.5%, 3h is stirred, filters, take filter
Slag, take beta-schardinger dextrin powder in mass ratio 1：10 add dimethyl sulfoxide mixing, obtain mixed solution, take filter residue in mass ratio 20：30：
1 adds mixed solution and stannous octoate, and 2 h are reacted in 25 DEG C, attraction, magnetic-particle B is collected, is washed with absolute ethyl alcohol,
Freeze-drying, obtains Magnetic Materials；
（4）Take step（1）Standby yolk powder matrix thing in mass ratio 1：10 add deionized water mixing, and pH is to 6.5 for regulation, then
The Magnetic Materials of deionized water quality 12% are added, 3h is kept in 30 DEG C, centrifugation, supernatant is taken, attraction, vibrates 60min, return
Receive Magnetic Materials, egg yolk liquid after must handling；
（5）According to the mass fraction, take 150 parts processing after egg yolk liquid, 5 portions of maltose, 6 parts of pectin, 90 parts of water, 30 parts of aid nutritions
Additive, 40min is stirred in 40 DEG C, is spray-dried, obtains the yolk powder for treating hypertension.
Comparative example：The yolk powder of Jinan bio tech ltd production
Method：According to standard DB43/T 1199-2016, the heat resistance of yolk powder prepared by detection embodiment and comparative example etc.
Property.
Test specific method：Each 8g of yolk powder prepared by the embodiment and comparative example of equivalent is taken, is put into beaker, is added
More than the 85 DEG C mL of hot water 200 are reconstituted, and stir 20s, are stood 15min, are detected its heat endurance.
Take the yolk powder prepared by the embodiment and comparative example of equivalent to add water that egg yolk liquid is made, it is passed through into 1000 r/min
5 min are centrifuged, detect its stability.
The specific detection case such as table 1 such as yolk powder heat-resistant stability
Table 1
Select 4 clinical observations of hyperpietic.Embodiment and the yolk powder prepared by comparative example are taken respectively, and instructions of taking is equal
To reconstitute 15ml every time, three times a day, the result of the test detection of one week laggard promoting circulation of blood pressure.
Alleviate the specific detection case such as table 2 of the yolk powder of hypertension
Table 2
Note：The blood pressure detecting value of experimenter is respectively before experiment：160/100mmHg, 158/100mmH, 161/100mmHg, 162/
100mmHg。
From the foregoing, it will be observed that the yolk powder hypotensive for hypotensive prepared by the present invention works well, and its heat resistance
Stability is high, and preparation method is simple to operation, is a kind of safety and the preparation method of the yolk powder of efficient hypotensive, is worth
Promote and use.
Claims (4)
1. a kind of preparation method for being used to treat the yolk powder of hypertension, it is characterised in that the preparation method comprises the following steps：
Take egg yolk in mass ratio 1：6 add absolute ethyl alcohol mixing, extraction, take extract to centrifuge, take precipitation rotary evaporation, cold
It is lyophilized dry, obtain degreasing thing, extracting degreasing thing 1：5 add distilled water mixing, add alkali protease and phospholipase A2, enzymolysis, obtain
Enzymolysis liquid, take enzymolysis liquid in mass ratio 5：2 add in 0.1mol/L sodium hydroxide solution, are stirred, obtain yolk powder matrix,
It is standby；
Take ferrous sulfate, iron chloride in mass ratio 1：3：12 ~ 15, which add the ethanol solution that mass fraction is 70%, mixes, and is passed through nitrogen
Gas, stirring, add mass fraction be 70% ethanol solution quality 40 ~ 50% ammoniacal liquor, keep 20 ~ 30 min, attraction,
Magnetic-particle A is collected, is washed respectively with ethanol and deionized water, is freeze-dried, obtains magnetic matrix thing；
Take chitosan in mass ratio 1：50, which add the acetic acid that mass fraction is 2%, mixes, and obtains mixed solution, mixed solution is pressed into matter
Measure ratio 10：7 add in magnetic matrix thing, are stirred, and add 4,4'- dicyclohexyl methyl hydride diisocyanates and octanoic acid is sub-
Tin, it is stirred, filters, take filter residue, take beta-schardinger dextrin powder in mass ratio 1：10 add dimethyl sulfoxide mixing, must mix molten
Liquid, take filter residue in mass ratio 20：30：1 adds mixed solution and stannous octoate, reaction, attraction, collects magnetic-particle B, uses
Absolute ethyl alcohol washs, and freeze-drying, obtains Magnetic Materials；
Take step（1）Standby yolk powder matrix thing in mass ratio 1：10 add deionized water mixing, and pH is to 6 ~ 6.5 for regulation, then
The Magnetic Materials of deionized water quality 10 ~ 12% are added, 2 ~ 3h is kept in 25 ~ 30 DEG C, centrifugation, supernatant is taken, attraction, vibrates,
Reclaim Magnetic Materials, egg yolk liquid after must handling；
According to the mass fraction, take 100 ~ 150 parts processing after egg yolk liquid, 4 ~ 5 portions of maltose, 5 ~ 6 parts of pectin, 70 ~ 90 parts of water, 20 ~
30 parts of aid nutrition additives, are stirred, and spray drying, obtain the yolk powder for treating hypertension.
2. the preparation method according to claim 1 for being used to treat the yolk powder of hypertension, it is characterised in that the step
（1）Neutral and alkali protease and phospholipase A2Addition be fat-free mass amount 6 ~ 7% alkali protease and fat-free mass amount 2 ~ 3%
Phospholipase A2；Enzymatic hydrolysis condition is：2 ~ 3h is digested in 50 ~ 55 DEG C.
3. the preparation method according to claim 1 for being used to treat the yolk powder of hypertension, it is characterised in that the step
（3）The addition of middle 4,4'- dicyclohexyl methyl hydride diisocyanates and stannous octoate is：Respectively magnetic matrix amount of substance 7 ~ 9%
With 0.2 ~ 0.5%.
4. the preparation method according to claim 1 for being used to treat the yolk powder of hypertension, it is characterised in that the step
（5）Middle health-care assistance additive：Take the root of kudzu vine, ampelopsis grossdentata leaf, chrysanthemum indicum to clean, according to the mass fraction, take 6 ~ 10 parts of the root of kudzu vine,
4 ~ 6 parts, 12 ~ 15 parts chrysanthemum indicums of ampelopsis grossdentata leaf, 20 ~ 25 parts of sterilized waters, homogenate, obtain homogenate, take Lactobacillus plantarum by 4%
Inoculum concentration be seeded in homogenate, in 38 ~ 42 DEG C ferment 1 ~ 2 day, obtain zymotic fluid, produce health-care assistance additive.
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|---|---|---|---|---|
| JPS6251092B2 (en) * | 1983-01-25 | 1987-10-28 | Q P Corp | |
| CN102960774A (en) * | 2012-12-07 | 2013-03-13 | 北京二商健力食品科技有限公司 | Instant yolk powder and preparation method thereof |
| CN105360995A (en) * | 2015-12-10 | 2016-03-02 | 江南大学 | Method for reducing cholesterol content of yolk |
-
2017
- 2017-12-16 CN CN201711356183.0A patent/CN107836662A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6251092B2 (en) * | 1983-01-25 | 1987-10-28 | Q P Corp | |
| CN102960774A (en) * | 2012-12-07 | 2013-03-13 | 北京二商健力食品科技有限公司 | Instant yolk powder and preparation method thereof |
| CN105360995A (en) * | 2015-12-10 | 2016-03-02 | 江南大学 | Method for reducing cholesterol content of yolk |
Non-Patent Citations (1)
| Title |
|---|
| 苏蕾，等: ""葛根发酵液对原发性高血压大鼠的降血压作用"，苏蕾，等，食品安全质量检测学报，第7卷第10期，第3925-3928页，2016年12月", 《食品安全质量检测学报》 * |
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