CN107822133A - 一种植物多酚改性黄精膳食纤维的制备方法 - Google Patents
一种植物多酚改性黄精膳食纤维的制备方法 Download PDFInfo
- Publication number
- CN107822133A CN107822133A CN201711247540.XA CN201711247540A CN107822133A CN 107822133 A CN107822133 A CN 107822133A CN 201711247540 A CN201711247540 A CN 201711247540A CN 107822133 A CN107822133 A CN 107822133A
- Authority
- CN
- China
- Prior art keywords
- sealwort
- dietary fiber
- modified
- plant polyphenol
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 55
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 49
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000008569 process Effects 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 7
- 239000002002 slurry Substances 0.000 claims description 30
- 102000004190 Enzymes Human genes 0.000 claims description 21
- 108090000790 Enzymes Proteins 0.000 claims description 21
- 229940088598 enzyme Drugs 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 238000003756 stirring Methods 0.000 claims description 17
- 238000001816 cooling Methods 0.000 claims description 12
- 230000009849 deactivation Effects 0.000 claims description 12
- 239000012043 crude product Substances 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 9
- 238000007873 sieving Methods 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 6
- 239000004365 Protease Substances 0.000 claims description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 6
- 102000004139 alpha-Amylases Human genes 0.000 claims description 5
- 108090000637 alpha-Amylases Proteins 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 claims description 4
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 claims description 4
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 claims description 2
- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 claims description 2
- ATJXMQHAMYVHRX-CPCISQLKSA-N Ellagic acid Natural products OC1=C(O)[C@H]2OC(=O)c3cc(O)c(O)c4OC(=O)C(=C1)[C@H]2c34 ATJXMQHAMYVHRX-CPCISQLKSA-N 0.000 claims description 2
- 229920002079 Ellagic acid Polymers 0.000 claims description 2
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 claims description 2
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 claims description 2
- 229930014669 anthocyanidin Natural products 0.000 claims description 2
- 150000001452 anthocyanidin derivatives Chemical class 0.000 claims description 2
- 235000008758 anthocyanidins Nutrition 0.000 claims description 2
- 235000005487 catechin Nutrition 0.000 claims description 2
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 claims description 2
- 229950001002 cianidanol Drugs 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims description 2
- 229910021641 deionized water Inorganic materials 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 235000004132 ellagic acid Nutrition 0.000 claims description 2
- 229960002852 ellagic acid Drugs 0.000 claims description 2
- 235000004515 gallic acid Nutrition 0.000 claims description 2
- 229940074391 gallic acid Drugs 0.000 claims description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 claims description 2
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 claims description 2
- 239000000047 product Substances 0.000 claims description 2
- 235000005875 quercetin Nutrition 0.000 claims description 2
- 229960001285 quercetin Drugs 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims 1
- 229930182470 glycoside Natural products 0.000 claims 1
- 150000002338 glycosides Chemical class 0.000 claims 1
- 230000004048 modification Effects 0.000 abstract description 3
- 238000012986 modification Methods 0.000 abstract description 3
- 229920001282 polysaccharide Polymers 0.000 abstract description 3
- 239000005017 polysaccharide Substances 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract description 2
- 235000012762 dietary quality Nutrition 0.000 abstract description 2
- 150000004676 glycans Chemical class 0.000 abstract description 2
- 238000005192 partition Methods 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 238000005194 fractionation Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 25
- 241000220225 Malus Species 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 206010013554 Diverticulum Diseases 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Chemical class CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 1
- 150000004056 anthraquinones Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 229930003944 flavone Chemical class 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- -1 polysaccharide carbon hydrate Chemical class 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 150000005856 steroid saponins Chemical class 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
本发明公开了一种植物多酚改性黄精膳食纤维的制备方法，包括打浆、酶解等过程，本发明利用植物多酚与多糖的反应，首次制备了植物多酚改性黄精膳食纤维，所述的改性黄精膳食纤维兼有黄精膳食纤维、植物多酚二者的优点；(2)采用两步酶处理分离法制备黄精膳食纤维，减少传统的粗分离法、化学分离法对膳食纤维中有效成分以及品质的破坏和对环境的污染，同时利用植物多酚可将可溶性黄精膳食纤维沉降，提高黄精的综合利用价值。
Description
技术领域
本发明涉及一种植物多酚改性黄精膳食纤维的制备方法，属于本发明涉及膳食纤维技术领域。
技术背景
1976年，英国营养病学专家H.C.Trowell将“不被人体消化吸收的多糖类碳水化合物与木质素”定义为“膳食纤维”。膳食纤维在现代营养学中被称为“第七大营养素”。膳食纤维有预防肥胖症、防治糖尿病、调节血脂水平、预防结肠癌、抑制有毒发酵产物、预防肠憩室和乳腺癌等生理功能。膳食纤维中的酸性多糖类具有较强的阳离子交换功能力，能与肠道中的Na+、K+进行交换,对消化道的pH值、渗透压及氧化电位产生影响，使血液中的Na+、K+比值降低,产生降血压作用。
膳食纤维提取改性活化的方法有很多种，常用的膳食纤维改性处理有生物技术方法(酶法、发酵法)、化学法(主要有酸法、碱法)和物理法(机械降解处理法)等。其中，利用化学法如酸法或碱法通过反复的浸泡和频繁的热处理过程，会明显减少纤维素产品的持水力和膨胀性，而且所排放的酸性或碱性工业废液对环境造成严重污染；利用物理法如机械降解处理法膳食纤维的得率不高；利用生物技术方法如酶法或发酵法处理综合效果最佳。
黄精是一种传统的药食同源性植物原料，富含淀粉、维生素、矿物质元素和多种活性物质。黄精具有补脾润肺、养阴生津、补中益气等功效和抑菌、抗病毒、抗肿瘤、抗衰老以及降糖降脂等作用，黄精的主要活性成分有糖类、甾体皂苷、黄酮、蒽醌类化合物、氨基酸和微量元素。但现有文献报道中很少关于研究提取、改性黄精膳食纤维。
植物多酚是多羟基酚类化合物的总称，又称为单宁，广泛存在于蔬菜、水果、豆类、谷物类、茶等植物中。多酚的独特结构赋予了它一些列独特的化学性质，使其具有抗肿瘤、抗氧化、抗动脉硬化、防治冠心病与中风等心脑血管疾病以及抗菌等多种生理功能。本发明利用植物多酚与黄精膳食纤维之间的反应，将二者的功效结合起来。
发明内容
为克服现有技术存在不足，本发明旨在提供一种植物多酚改性黄精膳食纤维的制备方法，包括如下步骤：
(1)将100重量份洗净后的黄精切片、500～1000重量份的水加入到磨浆机中磨浆18～30min，然后50～70℃加热下搅拌0.5～1h，冷却得到浆液；将浆液打入高压均质机中，1000～2000bar压力下剪切均质3～5次，得到稳定浆液。
(2)向(1)中稳定浆液中加入0.01～0.03重量份酶活为1200～2400AU的蛋白酶，pH＝5.0～7.0、搅拌下、40～60℃酶解1～3h，酶解充分后煮沸0.5～1h灭酶，然后冷却。
(3)向经(2)步骤处理后的浆液中加入0.05～0.3重量份30000～60000u/mlα-淀粉酶，pH＝5.0～7.0、搅拌下、40～60℃酶解1～3h，酶解充分后煮沸0.5～1h灭酶；然后60～80℃下加入15～30份植物多酚反应3～6h，经冷却、离心过滤后，沉淀即为改性黄精膳食纤维粗产品。
(4)将步骤(3)中所得改性黄精膳食纤维粗产品加水水洗，过滤，50～65℃干燥沉淀，再经粉碎、过筛后即得植物多酚改性黄精膳食纤维。
所处的水为蒸馏水或去离子水。
所述的乙醇为分析级。
所述的植物多酚为茶多酚、苹果多酚、葡萄多酚、花青素、儿茶素、栎精、没食子酸、鞣花酸、熊果苷中的至少一种。
所述的过筛为过100～400μm筛。
相对于现有技术，本发明具有以下优点：(1)利用植物多酚与多糖的反应，首次制备了植物多酚改性黄精膳食纤维，所述的改性黄精膳食纤维兼有黄精膳食纤维、植物多酚二者的优点；(2)采用两步酶处理分离法制备黄精膳食纤维，减少传统的粗分离法、化学分离法对膳食纤维中有效成分以及品质的破坏和对环境的污染，同时利用植物多酚可将可溶性黄精膳食纤维沉降，提高黄精的综合利用价值；(3)所述的制备方法中的时间、温度、pH值、均质压力等协同作用，使得所述的植物多酚改性黄精膳食纤维性能最优，且适口性较好。
具体实施方式
下面结合实施例对本发明的一种植物多酚改性黄精膳食纤维的制备方法做进一步的描述。可以理解的是，此处所描述的具体实施例仅仅用于解释相关发明，而非对该发明的限定。
实施例1
一种植物多酚改性黄精膳食纤维的制备方法，包括如下步骤：
(1)将100重量份洗净后的黄精切片、600重量份的水加入到磨浆机中磨浆20min，然后55℃加热下搅拌0.6h，冷却得到浆液；将浆液打入高压均质机中，1200bar压力下剪切均质4次，得到稳定浆液。
(2)向(1)中稳定浆液中加入0.02重量份酶活为1600AU的蛋白酶，pH＝6.0、搅拌下、50℃酶解1.5h，酶解充分后煮沸0.7h灭酶，然后冷却。
(3)向经(2)步骤处理后的浆液中加入0.15重量份40000u/mlα-淀粉酶，pH＝6.0、搅拌下、48℃酶解1.5h，酶解充分后煮沸0.7h灭酶；然后80℃下加入30份茶多酚反应3h，再经冷却、离心过滤后，沉淀即为改性黄精膳食纤维粗产品。
(4)将步骤(3)中所得改性黄精膳食纤维粗产品加水水洗，过滤，62℃干燥沉淀，再经粉碎、过筛后即得植物多酚改性黄精膳食纤维。
实施例2
一种植物多酚改性黄精膳食纤维的制备方法，包括如下步骤：
(1)将100重量份洗净后的黄精切片、1000重量份的水加入到磨浆机中磨浆30min，然后70℃加热下搅拌0.5h，冷却得到浆液；将浆液打入高压均质机中，2000bar压力下剪切均质3次，得到稳定浆液。
(2)向(1)中稳定浆液中加入0.03重量份酶活为2400AU的蛋白酶，pH＝7.0、搅拌下、60℃酶解1h，酶解充分后煮沸0.5h灭酶，然后冷却。
(3)向经(2)步骤处理后的浆液中加入0.3重量份30000u/mlα-淀粉酶，
pH＝7.0、搅拌下、60℃酶解1h，酶解充分后煮沸0.5h灭酶；然后60℃下加入24份苹果多酚反应6h，再经冷却、离心过滤后，沉淀即为改性黄精膳食纤维粗产品。
(4)将步骤(3)中所得改性黄精膳食纤维粗产品加水水洗，过滤，55℃干燥沉淀，再经粉碎、过筛后即得植物多酚改性黄精膳食纤维。
实施例3
一种植物多酚改性黄精膳食纤维的制备方法，包括如下步骤：
(1)将100重量份洗净后的黄精切片、500重量份的水加入到磨浆机中磨浆18min，然后50℃加热下搅拌1h，冷却得到浆液；将浆液打入高压均质机中，1000bar压力下剪切均质5次，得到稳定浆液。
(2)向(1)中稳定浆液中加入0.01重量份酶活为1200AU的蛋白酶，pH＝5.0、搅拌下、40℃酶解3h，酶解充分后煮沸1h灭酶，然后冷却。
(3)向经(2)步骤处理后的浆液中加入0.05重量份30000u/mlα-淀粉酶，pH＝5.0、搅拌下、40℃酶解3h，酶解充分后煮沸1h灭酶；然后70℃下加入15份茶多酚反应4h，经冷却、离心过滤后，沉淀即为改性黄精膳食纤维粗产品。
(4)将步骤(3)中所得改性黄精膳食纤维粗产品加水水洗，过滤，65℃干燥沉淀，再经粉碎、过筛后即得植物多酚改性黄精膳食纤维。
实施例4
一种植物多酚改性黄精膳食纤维的制备方法，包括如下步骤：
(1)将100重量份洗净后的黄精切片、800重量份的水加入到磨浆机中磨浆26min，然后65℃加热下搅拌0.8h，冷却得到浆液；将浆液打入高压均质机中，1600bar压力下剪切均质4次，得到稳定浆液。
(2)向(1)中稳定浆液中加入0.028重量份酶活为2000AU的蛋白酶，pH＝6.0、搅拌下、55℃酶解2.5h，酶解充分后煮沸0.6h灭酶，然后冷却。
(3)向经(2)步骤处理后的浆液中加入0.2重量份50000u/mlα-淀粉酶，pH＝6.0、搅拌下、50℃酶解2.5h，酶解充分后煮沸0.8h灭酶；然后75℃下加入15～25份苹果多酚反应5h，经冷却、离心过滤后，沉淀即为改性黄精膳食纤维粗产品。
(4)将步骤(3)中所得改性黄精膳食纤维粗产品加水水洗，过滤，55℃干燥沉淀，再经粉碎、过筛后即得植物多酚改性黄精膳食纤维。
实施例1-4中所述的植物多酚改性黄精膳食纤维的制备方法检测数据如下表：
Claims (5)
1.一种植物多酚改性黄精膳食纤维的制备方法，其特征在于：包括如下步骤：
(1)将100重量份洗净后的黄精切片、500～1000重量份的水加入到磨浆机中磨浆18～30min，然后50～70℃加热下搅拌0.5～1h，冷却得到浆液；将浆液打入高压均质机中，1000～2000bar压力下剪切均质3～5次，得到稳定浆液；
(2)向(1)中稳定浆液中加入0.01～0.03重量份酶活为1200～2400AU的蛋白酶，pH＝5.0～7.0、搅拌下、40～60℃酶解1～3h，酶解充分后煮沸0.5～1h灭酶，然后冷却；
(3)向经(2)步骤处理后的浆液中加入0.05～0.3重量份30000～60000u/mlα-淀粉酶，pH＝5.0～7.0、搅拌下、40～60℃酶解1～3h，酶解充分后煮沸0.5～1h灭酶；然后60～80℃下加入15～30份植物多酚反应3～6h，经冷却、离心过滤后，沉淀即为改性黄精膳食纤维粗产品；
(4)将步骤(3)中所得改性黄精膳食纤维粗产品加水水洗，过滤，50～65℃干燥沉淀，再经粉碎、过筛后即得植物多酚改性黄精膳食纤维。
2.根据权利要求1所述的一种植物多酚改性黄精膳食纤维的制备方法，其特征在于：所处的水为蒸馏水或去离子水。
3.根据权利要求1所述的一种植物多酚改性黄精膳食纤维的制备方法，其特征在于：所述的乙醇为分析级。
4.根据权利要求1所述的一种植物多酚改性黄精膳食纤维的制备方法，其特征在于：所述的植物多酚为茶多酚、苹果多酚、葡萄多酚、花青素、儿茶素、栎精、没食子酸、鞣花酸、熊果苷中的至少一种。
5.根据权利要求1所述的一种植物多酚改性黄精膳食纤维的制备方法，其特征在于：所述的过筛为过100～400μm筛。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711247540.XA CN107822133A (zh) | 2017-12-01 | 2017-12-01 | 一种植物多酚改性黄精膳食纤维的制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711247540.XA CN107822133A (zh) | 2017-12-01 | 2017-12-01 | 一种植物多酚改性黄精膳食纤维的制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107822133A true CN107822133A (zh) | 2018-03-23 |
Family
ID=61647490
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711247540.XA Pending CN107822133A (zh) | 2017-12-01 | 2017-12-01 | 一种植物多酚改性黄精膳食纤维的制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107822133A (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109222096A (zh) * | 2018-09-17 | 2019-01-18 | 南昌大学 | 胡萝卜膳食纤维中结合多酚的提取方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651465A (zh) * | 2004-02-04 | 2005-08-10 | 陈建操 | 糖基修饰改性膳食纤维及其制备方法和应用 |
| CN104479035A (zh) * | 2014-11-24 | 2015-04-01 | 云南中医学院 | 一种具有糖、脂代谢调节活性的滇黄精多糖提取物及其制备方法 |
| CN106749725A (zh) * | 2016-12-12 | 2017-05-31 | 浙江大学宁波理工学院 | 一种用超声波提取黄精中多糖的方法 |
-
2017
- 2017-12-01 CN CN201711247540.XA patent/CN107822133A/zh active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651465A (zh) * | 2004-02-04 | 2005-08-10 | 陈建操 | 糖基修饰改性膳食纤维及其制备方法和应用 |
| CN104479035A (zh) * | 2014-11-24 | 2015-04-01 | 云南中医学院 | 一种具有糖、脂代谢调节活性的滇黄精多糖提取物及其制备方法 |
| CN106749725A (zh) * | 2016-12-12 | 2017-05-31 | 浙江大学宁波理工学院 | 一种用超声波提取黄精中多糖的方法 |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109222096A (zh) * | 2018-09-17 | 2019-01-18 | 南昌大学 | 胡萝卜膳食纤维中结合多酚的提取方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103719880B (zh) | 高活性紫甘薯膳食纤维的制备方法 | |
| CN106905440B (zh) | 一种银耳多糖提取的方法 | |
| CN103263514B (zh) | 一种柑橘皮黄酮、低分子果胶和纤维素的联合提取方法 | |
| CN101455357A (zh) | 用超滤结合喷雾干燥制备大豆渣水溶性膳食纤维的方法 | |
| CN106343410A (zh) | 一种海带提取物的生产方法 | |
| CN113501892B (zh) | 一种柑橘类果实皮渣中不同功能性成分的联合提取方法 | |
| CN104710541A (zh) | 一种从海带中制备海带多糖的方法 | |
| CN104886581A (zh) | 降尿酸、治疗痛风及去营养过剩生物制剂及其制备方法 | |
| CN109288072A (zh) | 一种柑果渣纳米膳食纤维的制备方法 | |
| CN105996028B (zh) | 生姜提取物、生姜纤维及其制备方法 | |
| CN102492054B (zh) | 一种大豆种皮果胶类凝胶多糖的制备方法 | |
| CN103238892A (zh) | 雪莲果原汁及原汁粉的生产方法 | |
| CN104757564A (zh) | 一种利用花生壳制备膳食纤维的方法 | |
| CN106912964A (zh) | 可溶性膳食纤维及其制备方法 | |
| CN102321699A (zh) | 复合酶解辅助提取蜂胶中有效成份的方法 | |
| CN105054079B (zh) | 一种甘薯加工利用方法 | |
| CN106282266A (zh) | 一种利用柚苷酶制备浒苔寡糖的方法 | |
| CN107836686A (zh) | 一种海藻碘营养液及海带调味粉联合生产方法 | |
| CN114568709A (zh) | 一种提高方竹笋水溶性膳食纤维含量的提取方法 | |
| CN107286264A (zh) | 大枣营养物质分离的深加工方法 | |
| CN102356845B (zh) | 一种米糠营养素的制备方法 | |
| CN107822133A (zh) | 一种植物多酚改性黄精膳食纤维的制备方法 | |
| CN111909285A (zh) | 一种黑木耳多糖及其应用和制备方法 | |
| CN113694152B (zh) | 一种高稳定性酶解法获取薏苡仁提取液的方法 | |
| CN101731576A (zh) | 一种从牛蒡渣中制备纳米级膳食纤维的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180323 |
|
| RJ01 | Rejection of invention patent application after publication |