CN107811232B - Kelp compound microorganism deodorization method - Google Patents

Kelp compound microorganism deodorization method Download PDF

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Publication number
CN107811232B
CN107811232B CN201710981772.1A CN201710981772A CN107811232B CN 107811232 B CN107811232 B CN 107811232B CN 201710981772 A CN201710981772 A CN 201710981772A CN 107811232 B CN107811232 B CN 107811232B
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kelp
saccharomyces cerevisiae
lactobacillus
fishy smell
fermentation
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CN107811232A (en
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李维新
何志刚
林晓姿
任香芸
梁璋成
苏昊
林晓婕
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Fujian Academy Of Agricultural Sciences Agricultural Product Processing Research Institute
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Institute of Agricultural Engineering Technology of Fujian Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/60Edible seaweed
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/27Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/27Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
    • A23L5/276Treatment with inorganic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/28Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Meat, Egg Or Seafood Products (AREA)

Abstract

The invention provides a kelp compound microorganism deodorization method, which comprises the following steps: adding herba Zosterae Marinae into hot water containing calcium lactate 0.3-0.4%, and blanching at 95-100 deg.C for 3-4 min; cooling, draining, placing into a sterilized container, adding sugar and saline water, wherein the sugar and saline water contains 1.5-2% of sucrose, 6-7% of salt and the balance of water by weight; adding composite microorganism composed of Saccharomyces cerevisiae and lactobacillus, and fermenting at 30-32 deg.C for 8-10 days; and (5) fishing out the kelp, and draining. According to the technical scheme, the saccharomyces cerevisiae for producing the aroma and the lactic acid bacteria with the malic acid and lactic acid fermentation functions are used for carrying out microbial mixed fermentation on the kelp, so that the content of substances with the fishy smell of the kelp can be reduced, the content of the fragrant substances can be increased, and the special fermentation aroma is generated. The kelp raw material after the fishy smell is removed by the technology can be processed into instant kelp or other kelp-like products. The technical scheme has simple steps, is easy for industrial production and is beneficial to the development of the kelp deep processing industry.

Description

Kelp compound microorganism deodorization method
Technical Field
The invention relates to the field of food processing, in particular to a kelp compound microorganism deodorization method.
Background
The laminarin has the reputations of longevity vegetable, marine vegetable and iodine-containing champion, is a food with health care and longevity, is an important economic seaweed in China, and plays an important role in the domestic aquatic product industry, the culture scale and the culture yield of the laminarin are the first in the world, the annual yield accounts for 81-88% of the annual yield of the world, and the output value accounts for 81-90% of the total value of the world. However, kelp has a special fishy smell, which makes processing difficult and is unacceptable to consumers. The main cause of the fishy smell of kelp is that kelp contains various components such as amines, terpenoids, carboxylic acids, alkanes and the like.
At present, the methods for removing the fishy smell of the kelp mainly comprise an embedding method, an adsorption method, a flavor masking method, citric acid deodorization, acetic acid deodorization and the like, but the methods basically hardly reduce or change the fishy smell of the kelp, so that the fishy smell of the kelp is recovered in the further deep processing process of the kelp, and the taste is influenced.
Disclosure of Invention
Therefore, a method for effectively reducing the fishy smell of the kelp is needed, so that the kelp can be better deodorized. In order to achieve the aim, the inventor provides a kelp compound microorganism fishy smell removing method, which comprises the following steps:
s1: putting herba Zosterae Marinae into hot water containing 0.3-0.4 wt% of calcium lactate, and blanching at 95-100 deg.C for 3-4 min;
s2: cooling the blanched kelp, draining, putting into a sterilized container, and adding a salt sugar solution into the container, wherein the salt sugar solution contains 1.5-2% of sucrose, 6-7% of salt and the balance of water;
s3: adding composite microorganism composed of Saccharomyces cerevisiae and lactobacillus into the container, and fermenting at 30-32 deg.C for 8-10 days;
s4: and (5) fishing out the kelp, and draining.
Further, in the step S1, the kelp is cut into a filament or a strip.
Further, the material of the container in the step S2 is stainless steel, and the container is sterilized by steam sterilization and then used.
Further, the saline solution prepared in the step S2 is heated to 100 ℃ for sterilization, and is used after being cooled.
Further, in the step S2, the weight ratio of the drained kelp to the salt sugar water is 1.5-2.0: 1.
further, in the step S3, the weight ratio of the saccharomyces cerevisiae and the lactobacillus dried powder is 1-1.5: 2.
Further, in the step S3, the saccharomyces cerevisiae is saccharomyces cerevisiae JP2 or J4, and the lactic acid bacteria is lactobacillus plantarum R23.
Further, in the step S3, the weight ratio of the composite microbial dry bacterial powder to the kelp and saline sugar water mixture is 0.5-1: 1000.
Different from the prior art, the technical scheme adopts the method for removing the fishy smell by the saccharomyces cerevisiae and lactobacillus composite microorganisms, and the kelp is subjected to microbial fermentation by the saccharomyces cerevisiae capable of producing fragrance and the lactobacillus with the malic acid and lactic acid fermentation functions, so that the content of fishy smell substances of the kelp can be reduced, the content of fragrant substances can be increased, and the special fermentation fragrance can be generated. The kelp raw material after the fishy smell is removed by the technology can be directly processed into instant kelp or other kelp-like products. The technical scheme has simple steps, is easy for industrial production and is beneficial to the development of the kelp deep processing industry.
Detailed Description
In order to explain technical contents, structural features, and objects and effects of the technical means in detail, the following detailed description is given with reference to specific embodiments.
In the embodiment, the saccharomyces cerevisiae is saccharomyces cerevisiae JP2(s.cerevisiae JP2) and saccharomyces cerevisiae J4(s.cerevisiae J4), and is selected and bred by research institute of agricultural engineering technology of deep academy of agricultural sciences of fujian, and the patent authorization publication numbers are CN 102154135B and CN 102168027B, respectively.
In the embodiment, the lactobacillus plantarum R23(L.plantarum R23) is a lactic acid bacterium (NCBI: HQ658056) bred by the research institute of agricultural engineering technology of agricultural academy of sciences of Fujian province. The strain is preserved in China general microbiological culture Collection center (CGMCC), and the preservation number is as follows: lactobacillus plantarum CGMCC No. 5105. The preservation date is as follows: day 1 month 8 2011, latten name by category: lactobacillus plantarum.
Example 1:
cutting fresh herba Zosterae Marinae into filaments or strips, adding into hot water containing calcium lactate 0.3%, blanching at 95 deg.C for 4min, taking out, cooling, draining water, and placing into steam sterilized stainless steel barrel with a loading amount of 60% of the barrel volume.
Preparing sugar saline water: 1.5 percent of sucrose, 6 percent of salt and the balance of water, heating to 100 ℃ for sterilization after being prepared, and cooling for use. Adding sugar saline water into a stainless steel barrel according to the weight ratio of the kelp to the sugar saline water of 1.5: 1.
Adding saccharomyces cerevisiae JP2 and lactobacillus R23 into the container, wherein the weight ratio of dry bacterial powder of the saccharomyces cerevisiae JP2 to lactobacillus R23 is 1:1.5, and the total amount of the composite microbial dry bacterial powder is 0.1 percent of the total weight of the kelp and the saline water. After the addition of the microorganisms, the stainless steel drum was sealed with 2 layers of gauze and left to ferment at 30-32 ℃ for 8 days. After the fermentation is finished, the kelp is fished and drained.
In the operation of example 1, volatile components in the kelp after and before fermentation were extracted using a solid phase microextraction head, and the odor components and relative contents in the kelp were analyzed by GC-MS.
Through NIST mass spectrum database retrieval and literature control, 103 components are detected in total. Wherein, substances with special fishy smell such as 1, 3-octadiene, E, Z-3-ethylidene cyclohexene, 3-methyl-6- (1-methylvinyl) -cyclohexene, n-heptanal, etc. are respectively reduced to 0%, 1.72% and 0.51% from 10.47%, 6.40%, 8.41% and 2.26% before fermentation. While the floral and ester flavors of ethyl octanoate, ethyl decanoate, beta-phenylethyl alcohol 0.72%, 0% increased to 2.26%, 2.04%, and 5.63%.
The test result proves that the composite microbial fermentation mode consisting of saccharomyces cerevisiae and lactic acid bacteria can effectively reduce the content of substances with the kelp fishy smell such as 1, 3-octadiene, E, Z-3-ethylidene cyclohexene, 3-methyl-6- (1-methylvinyl) -cyclohexene and n-heptanal, can increase the content of fragrant substances such as ethyl caprylate, ethyl caprate, beta-phenylethyl alcohol and the like, and can generate special fermentation fragrance.
Example 2:
cutting fresh herba Zosterae Marinae into filaments or strips, adding into hot water containing calcium lactate 0.4%, blanching at 100 deg.C for 3min, taking out, cooling, draining water, and placing into steam sterilized stainless steel barrel with a loading amount of 60% of the barrel volume.
Preparing sugar saline water: sucrose 2%, salt 7% and water in balance, heating to 100 deg.C for sterilization, and cooling for use. Adding sugar saline water into a stainless steel barrel according to the weight ratio of the kelp to the sugar saline water of 2.0: 1.
Adding saccharomyces cerevisiae JP2 and lactobacillus R23 into the container, wherein the weight ratio of dry bacterial powder of the saccharomyces cerevisiae JP2 to lactobacillus R23 is 1:2.0, and the total amount of the composite microbial dry bacterial powder is 0.05 percent of the total weight of the kelp and the saline water. After the addition of the microorganisms, the stainless steel drum was sealed with 2 layers of gauze and left to ferment at 30-32 ℃ for 10 days. After the fermentation is finished, the kelp is fished and drained.
Example 3:
cutting fresh herba Zosterae Marinae into filaments or strips, adding into hot water containing calcium lactate 0.35%, blanching at 97 deg.C for 3min, taking out, cooling, draining water, and placing into steam sterilized stainless steel barrel with a loading amount of 60% of the barrel volume.
Preparing sugar saline water: 1.8 percent of sucrose, 6.5 percent of salt and the balance of water, heating to 100 ℃ after being prepared, sterilizing and cooling for use. Adding sugar saline water into a stainless steel barrel according to the weight ratio of the kelp to the sugar saline water of 1.8: 1.
Adding saccharomyces cerevisiae JP2 and lactobacillus R23 into the container, wherein the weight ratio of dry bacterial powder of the saccharomyces cerevisiae JP2 to lactobacillus R23 is 1:1.8, and the total amount of the composite microbial dry bacterial powder is 0.08 percent of the total weight of the kelp and the saline water. After the addition of the microorganisms, the stainless steel drum was sealed with 2 layers of gauze and left to ferment at 30-32 ℃ for 9 days. After the fermentation is finished, the kelp is fished and drained.
Example 4:
cutting fresh herba Zosterae Marinae into filaments or strips, adding into hot water containing calcium lactate 0.4%, blanching at 100 deg.C for 3min, taking out, cooling, draining water, and placing into steam sterilized stainless steel barrel with a loading amount of 60% of the barrel volume.
Preparing sugar saline water: 2.0 percent of sucrose, 6.0 percent of salt and the balance of water, heating to 100 ℃ after being prepared, sterilizing and cooling for use. Adding sugar saline water into a stainless steel barrel according to the weight ratio of the kelp to the sugar saline water of 1.5: 1.
Adding saccharomyces cerevisiae J4 and lactobacillus R23 into the container, wherein the weight ratio of saccharomyces cerevisiae J4 to lactobacillus R23 dry bacteria powder is 1:2, and the total amount of the composite microbial dry bacteria powder is 0.1 percent of the total weight of the kelp and the saline water. After the addition of the microorganisms, the stainless steel drum was sealed with 2 layers of gauze and left to ferment at 30-32 ℃ for 8 days. After the fermentation is finished, the kelp is fished and drained.
Example 5:
cutting fresh herba Zosterae Marinae into filaments or strips, adding into hot water containing calcium lactate 0.3%, blanching at 100 deg.C for 3min, taking out, cooling, draining water, and placing into steam sterilized stainless steel barrel with a loading amount of 60% of the barrel volume.
Preparing sugar saline water: 2.0 percent of sucrose, 6.5 percent of salt and the balance of water, heating to 100 ℃ after being prepared, sterilizing and cooling for use. Adding sugar saline water into a stainless steel barrel according to the weight ratio of the kelp to the sugar saline water of 1.5: 1.
Adding saccharomyces cerevisiae J4 and lactobacillus R23 into the container, wherein the weight ratio of dry bacterial powder of the saccharomyces cerevisiae J4 to the lactobacillus R35 is 1:1.8, and the total amount of the composite microbial dry bacterial powder is 0.07 percent of the total weight of the kelp and the saline water. After the addition of the microorganisms, the stainless steel drum was sealed with 2 layers of gauze and left to ferment at 30-32 ℃ for 8 days. After the fermentation is finished, the kelp is fished and drained.
It should be noted that, although the above embodiments have been described herein, the invention is not limited thereto. Therefore, based on the innovative concepts of the present invention, the technical solutions of the present invention can be directly or indirectly applied to other related technical fields by making changes and modifications to the embodiments described herein or by using equivalent structures or equivalent processes performed in the present specification, and are included in the scope of the present invention.

Claims (7)

1. A kelp compound microorganism deodorization method is characterized by comprising the following steps:
s1: putting herba Zosterae Marinae into hot water containing 0.3-0.4 wt% of calcium lactate, and blanching at 95-100 deg.C for 3-4 min;
s2: cooling the blanched kelp, draining, putting into a sterilized container, and adding a salt sugar solution into the container, wherein the salt sugar solution contains 1.5-2% of sucrose, 6-7% of salt and the balance of water;
s3: adding a compound microorganism consisting of saccharomyces cerevisiae and lactobacillus into a container for fermentation, wherein the fermentation temperature is 30-32 ℃, the fermentation time is 8-10 days, the saccharomyces cerevisiae is saccharomyces cerevisiae JP2 or J4, and the lactobacillus is lactobacillus plantarum R23;
s4: and (5) fishing out the kelp, and draining.
2. The method according to claim 1, wherein in the step S1, the kelp is cut into a string or a long strip.
3. The method of claim 1, wherein the container of step S2 is made of stainless steel, and is sterilized by steam sterilization.
4. The fishy smell removal method of claim 1: the method is characterized in that the salt sugar water prepared in the step S2 is heated to 100 ℃ for sterilization and is used after being cooled.
5. The fishy smell removal method of claim 1: characterized in that in the step S2, the weight ratio of the drained kelp to the salt sugar water is 1.5-2.0: 1.
6. the fishy smell removal method of claim 1: the method is characterized in that in the step S3, the weight ratio of the saccharomyces cerevisiae to the lactobacillus dry bacteria powder is 1: 1.5-2.0.
7. The fishy smell removal method of claim 1: characterized in that in the step S3, the weight ratio of the composite microbial dry fungus powder to the mixture of kelp and salt sugar water is 0.5-1: 1000.
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Application publication date: 20180320

Assignee: Fujian Gelin Food Industry Group Co.,Ltd.

Assignor: INSTITUTE OF AGRICULTURAL ENGINEERING TECHNOLOGY, FUJIAN ACADEMY OF AGRICULTURAL SCIENCES

Contract record no.: X2023350000009

Denomination of invention: A method of removing fishy smell from kelp by compound microorganism

Granted publication date: 20200828

License type: Common License

Record date: 20230130

EE01 Entry into force of recordation of patent licensing contract
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Application publication date: 20180320

Assignee: FUJIAN RED SUN SUPERFINES CO.,LTD.

Assignor: INSTITUTE OF AGRICULTURAL ENGINEERING TECHNOLOGY, FUJIAN ACADEMY OF AGRICULTURAL SCIENCES

Contract record no.: X2023350000013

Denomination of invention: A method of removing fishy smell from kelp by compound microorganism

Granted publication date: 20200828

License type: Common License

Record date: 20230131

EE01 Entry into force of recordation of patent licensing contract
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Address after: No. 248 Wusi North Road, Fuzhou City, Fujian Province, 350003

Patentee after: Fujian Academy of Agricultural Sciences Agricultural Product Processing Research Institute

Address before: 350000 No. 54, 247 Gulou District, Fujian, Fuzhou

Patentee before: INSTITUTE OF AGRICULTURAL ENGINEERING TECHNOLOGY, FUJIAN ACADEMY OF AGRICULTURAL SCIENCES

CP03 Change of name, title or address