CN108902595A - A kind of preparation method of the fermentation of seaweed beverage rich in viable lactic acid bacteria - Google Patents

A kind of preparation method of the fermentation of seaweed beverage rich in viable lactic acid bacteria Download PDF

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Publication number
CN108902595A
CN108902595A CN201810632395.5A CN201810632395A CN108902595A CN 108902595 A CN108902595 A CN 108902595A CN 201810632395 A CN201810632395 A CN 201810632395A CN 108902595 A CN108902595 A CN 108902595A
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seaweed
fermentation
lactic acid
acid bacteria
preparation
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程文健
张卿
叶锐
陈丽娇
梁鹏
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135Bacteria or derivatives thereof, e.g. probiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/123Bulgaricus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/125Casei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/173Reuteri
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/175Rhamnosus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/513Adolescentes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/517Bifidum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/519Breve
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/529Infantis
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/533Longum

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Abstract

The preparation method of the present invention relates to a kind of fermentation of seaweed beverage rich in viable lactic acid bacteria, belongs to alga food manufacture field.Seaweed is starched preparation fermentation base fluid by the present invention, is then carried out lactic fermentation, then the aroma-producing yeasts of activation are added during the fermentation, is obtained the fermentation of seaweed beverage rich in viable lactic acid bacteria.The present invention passes through the effect of the coupling fermentation of lactic acid bacteria and aroma-producing yeasts, it is fragrant to assign the pure and fresh ester of product, effectively eliminate and be blinded by the fishy smell ingredient of seaweed, product, which does not need additionally to add food flavor or fishy-smell removing agent, has pure and fresh fragrance and free from extraneous odour, seaweed diet fiber and viable lactic acid bacteria rich in(Viable count of lactobacillus >=106CFU/g), there is good adjustment effect to human body intestinal canal health.

Description

A kind of preparation method of the fermentation of seaweed beverage rich in viable lactic acid bacteria
Technical field
The preparation method of the present invention relates to a kind of fermentation of seaweed beverage rich in viable lactic acid bacteria belongs to alga food processing Field.
Background technique
Seaweed refers to the algae being grown in seawater, including a variety of seaweed in Rhodophyta, Phaeophyta and Chlorophyta. These seaweed can much eat, such as seaweed, agar, fragrant plant mentioned in ancient texts in Rhodophyta, kelp, thallus laminariae in Phaeophyta and Enteromorpha, sea lettuce in sargassum etc. and Chlorophyta etc..Seaweed not only delicious flavour, there are also very high nutritive value, in seaweed Protein rich in, the several mineral materials such as algal polysaccharides, dietary fiber, multivitamin and iodine, iron, zinc, calcium, selenium, With lowering blood pressure and blood fat, anti-oxidant anti-aging, the multiple efficacies such as raising immunity, therefore seaweed has very high edible valence Value and health-care efficacy.All kinds of alga foods are made by processing methods such as marinated, drying, bakings in seaweed raw material majority at present.Closely Nian Lai develops ocean health food such as seaweed flavouring, algae beverage using seaweed, and the food such as marine alga wine become and realize seaweed One main path of resource higher value application.
Most lactic acid bacterias can be used as a kind of probiotics, has the function of to improve human gastrointestinal tract, restores flora in human body intestinal canal Balance forms antibacterial biological barrier, improves immunity, and the effect of human health is safeguarded in reducing blood lipid etc., existing at present much to contain The food of viable lactic acid bacteria is developed, such as Yoghourt, lactic acid bacteria beverage made of fruits or vegetables, lactic acid bacteria fruits and vegetables solid beverage, pickles etc..
Summary of the invention
The preparation method of the purpose of the present invention is to provide a kind of fermentation of seaweed beverage rich in viable lactic acid bacteria.
To achieve the above object, the present invention adopts the following technical scheme that:
A kind of preparation method of the fermentation of seaweed beverage rich in viable lactic acid bacteria, the specific steps are:
(1)The preparation of edible seaweed slurry:After edible dry seaweed or new fresh seaweed clear water wash clean, rubbed with meat grinder, then plus The drinking water for entering 5-20 parts of seaweed weights impregnates to be beaten with thick pulp grinder and screened stock machine respectively after sixty minutes, obtains average grain diameter≤10 μm edible seaweed screened stock.
(2)The preparation of fermentation of seaweed base fluid:Fermentation of seaweed base fluid is deployed according to the following formulation according to parts by weight:Seaweed slurry 30-60 parts, 10-60 parts of drinking water, 5-25 parts of sugar, 5-10 parts of maltitol, 0.1-10.0 parts of food thickening agent.It will match above Sugar in side, maltitol, food thickening agent after mixing, be added formula in water and heating for dissolving after and in formula Seaweed slurry is uniformly mixed, and after blend compounds body mill or high pressure homogenizer homogeneous, is heated to 100 DEG C and is kept the temperature 30 points at this temperature Clock, be cooled to 30-40 DEG C it is spare.
(3)The preparation of leavening:Lactic acid bacteria culturers are aseptically activated to and are expanded culture, after expanding culture Obtained viable count of lactobacillus reaches 107The lactobacillus suspension of CFU/g or more.In addition aroma-producing active dry yeast 10-20 times is added to do It in 2.5% liquid glucose of yeast weight, and is heated to 30-35 DEG C of heat preservation and activates 10 hours, obtain activated yeast bacterium solution.
(4)The preparation of fermentation of seaweed beverage:By step(2)Fermentation of seaweed base fluid obtained is added in fermentor, and sea is added The edible alcohol of algae fermentation base fluid weight 0.1-1%, and the lactobacillus suspension for accounting for fermentation of seaweed base fluid weight 0.1-1% is added and accounts for sea After the activated yeast bacterium solution of algae fermentation base fluid weight 0.05-1% is uniformly mixed and seals, adjusting temperature in fermentor is 30-40 DEG C, heat-preservation fermentation 24-48 hours, obtain the fermentation of seaweed liquid rich in biodiasmin.This fermentation liquid filling and package is placed on 4 Obtain the fermentation of seaweed beverage rich in viable lactic acid bacteria within after-ripening 18-48 hours in DEG C freezer.
The edible seaweed is Rhodophyta, Phaeophyta, any one edible seaweed in Chlorophyta, as kelp, Pelvetia silquosa, thallus laminariae, sargassum fusifome, agar, seaweed, U. pertusa, Enteromorpha etc..
The lactic acid bacteria culturers are lactobacillus plantarum, bifidobacterium bifidum, bifidobacterium longum, bifidobacterium infantis, blueness Spring Bifidobacterium, bifidobacterium breve, lactobacillus bulgaricus, L. casei casei, lactobacillus acidophilus, Luo Yishi cream bar Bacterium, Lactobacillus rhamnosus, streptococcus thermophilus it is one or more.
The aroma-producing active dry yeast is commercial goods, main component be special-shaped Hansenula yeast with ester producing capacity and A small number of small round Saccharomyces cerevisiaes.
Step(2)Sugar described in formula is one of sucrose, maltose, fructose, glucose, starch syrup or more Kind.
The food thickening agent is sodium alginate, xanthan gum, Arabic gum, locust bean gum, pectin, guar gum, knot One of cold glue, sodium carboxymethylcellulose are a variety of.
Remarkable advantage of the invention is:
1, edible seaweed is broken into screened stock of the average grain diameter less than 10 μm by the present invention, and by the fermentation of lactic acid bacteria, is helped In destroying alginic cell wall construction, promote the dissolution of the seaweed diet fibers such as the algal polysaccharides in alginic cell.
2, the aroma-producing yeasts of activation are added in the present invention in lactic fermentation process, pass through the coupling of lactic acid bacteria and aroma-producing yeasts Fermentation assigns product pure and fresh ester perfume, effectively eliminates and be blinded by the fishy smell ingredient of seaweed, product does not need additionally to add Food flavor or fishy-smell removing agent have pure and fresh fragrance and free from extraneous odour.
3, fermentation of seaweed beverage prepared by the present invention seaweed diet fiber rich in and viable lactic acid bacteria(Lactic acid bacteria is living Bacterium number >=106CFU/g), there is good adjustment effect to human body intestinal canal health.In addition, this product sour and sweet palatability, fragrance is coordinated, There is no the fishy smell of seaweed, is a kind of seaweed beverage of health.
Specific embodiment
Further to disclose rather than the present invention is limited, the present invention is described in further detail below in conjunction with example.
Embodiment 1
(1)The preparation of kelp paste:It after dry kelp clear water wash clean, is rubbed with meat grinder, adds the drink of 10 parts of kelp weight It is soaked in water and is beaten respectively with thick pulp grinder and screened stock machine after sixty minutes, obtain the edible seaweed screened stock of average grain diameter≤10 μm.
(2)Kelp fermentation base fluid preparation:Kelp fermentation base fluid is deployed according to the following formulation according to parts by weight:Kelp paste 50 parts, 30 parts of drinking water, 15 parts of sucrose, 5 parts of maltitol, 0.1 part of xanthan gum.By the sucrose in the above formula, maltitol, Xanthan gum after mixing, is uniformly mixed after water and heating for dissolving in formula is added with the kelp paste in formula, blend compounds body Mill or high pressure homogenizer homogeneous after, be heated to 100 DEG C and at this temperature heat preservation 30 minutes, be cooled to 30 DEG C it is spare.
(3)The preparation of leavening:Lactobacillus plantarum strain portion is aseptically activated and expanded culture, is expanded Viable count of lactobacillus is made after big culture and reaches 107The lactobacillus suspension of CFU/g or more.In addition the raw smoked bean curd yeast of high activity is added It in 2.5% liquid glucose of 15 times of dry ferment weight, and is heated to 30 DEG C of heat preservations and activates 10 hours, obtain activated yeast bacterium solution.
(4)The preparation of kelp fermented beverage:By step(2)Kelp fermentation base fluid obtained is added in fermentor, and sea is added Edible alcohol with fermentation base fluid 0.5%, and the lactobacillus suspension for accounting for kelp fermentation base fluid weight 0.5% is added and accounts for kelp fermentation base After the activated yeast bacterium solution of liquid weight 0.07% is uniformly mixed and seals, adjusting temperature in fermentor is 32 DEG C, and heat-preservation fermentation 18 is small When, obtain the kelp fermentation liquid rich in biodiasmin.It is small that this fermentation liquid filling and package is placed on after-ripening 24 in 4 DEG C of freezers When obtain the kelp fermented beverage rich in viable lactic acid bacteria.
After measured, reach 10 by viable count of lactobacillus in kelp fermented beverage made from the present embodiment7CFU/g。
Embodiment 2
(1)The preparation of seaweed slurry:It after dry laver clear water wash clean, is rubbed with meat grinder, adds the drink of 10 parts of seaweed weight It is soaked in water and is beaten respectively with thick pulp grinder and screened stock machine after sixty minutes, obtain the edible laver screened stock of average grain diameter≤10 μm.
(2)Seaweed fermentation base fluid preparation:Seaweed fermentation base fluid is deployed according to the following formulation according to parts by weight:Seaweed slurry 45 parts, 25 parts of drinking water, 20 parts of glucose, 5 parts of maltitol, 5 parts of Arabic gum.By glucose, the malt in the above formula Sugar alcohol, Arabic gum after mixing, are uniformly mixed after water and heating for dissolving in formula is added with the seaweed slurry in formula, and After colloid mill or high pressure homogenizer homogeneous, be heated to 90 DEG C and at this temperature keep the temperature 15 minutes, be cooled to 30 DEG C it is spare.
(3)The preparation of leavening:Lactobacillus plantarum, lactobacillus acidophilus are respectively taken into portion, aseptically activated It is cultivated with expanding, obtained viable count of lactobacillus reaches 10 after expanding culture7The lactobacillus suspension of CFU/g or more.In addition by high activity Raw smoked bean curd yeast is added in 2.5% liquid glucose of 10 times of dry ferment weight, and is heated to 35 DEG C of heat preservations and activates 10 hours, is activated Yeast liquid.
(4)The preparation of seaweed fermented beverage:By step(2)Seaweed fermentation base fluid obtained is added in fermentor, is added purple The edible alcohol of dish fermentation base fluid 0.8%, and the lactobacillus suspension for accounting for seaweed fermentation base fluid weight 0.8% is added and accounts for seaweed fermentation base After the activated yeast bacterium solution of liquid weight 0.07% is uniformly mixed and seals, adjusting temperature in fermentor is 35 DEG C, and heat-preservation fermentation 24 is small When, obtain the seaweed fermentation liquid rich in biodiasmin.It is small that this fermentation liquid filling and package is placed on after-ripening 36 in 4 DEG C of freezers When obtain the seaweed fermented beverage rich in viable lactic acid bacteria.
After measured, reach 10 by viable count of lactobacillus in seaweed fermented beverage made from the present embodiment7CFU/g。
Embodiment 3
(1)The preparation of U. pertusa slurry:It after edible dry hole sea lettuce clear water wash clean, is rubbed with meat grinder, adds 15 parts of Kong Shi The drinking water of water shield weight impregnates to be beaten with thick pulp grinder and screened stock machine respectively after sixty minutes, obtains the U. pertusa of average grain diameter≤10 μm Screened stock.
(2)U. pertusa fermentation base fluid preparation:U. pertusa fermentation base fluid is deployed according to the following formulation according to parts by weight:Hole Sea lettuce starches 40 parts, 40 parts of drinking water, 10 parts of starch syrup, 9.8 parts of maltitol, 0.2 part of locust bean gum.It will be in the above formula Starch syrup, maltitol, locust bean gum after mixing, be added formula in water and heating for dissolving after with the Kong Shi in formula Water shield slurry is uniformly mixed, and after blend compounds body mill or high pressure homogenizer homogeneous, is heated to 90 DEG C and is kept the temperature 15 minutes at this temperature, cold But spare to 30 DEG C.
(3)The preparation of leavening:Bifidobacterium breve, lactobacillus acidophilus are respectively taken into portion, aseptically activated It is cultivated with expanding, obtained viable count of lactobacillus reaches 10 after expanding culture7The lactobacillus suspension of CFU/g or more.In addition by high activity Raw smoked bean curd yeast is added in 2.5% liquid glucose of 15 times of dry ferment weight, and is heated to 35 DEG C of heat preservations and activates 10 hours, is activated Yeast liquid.
(4)The preparation of U. pertusa fermented beverage:By step(2)U. pertusa fermentation base fluid obtained is added in fermentor, is added Enter U. pertusa fermentation base fluid weight 0.2% edible alcohol, and be added account for U. pertusa fermentation base fluid weight 0.2% lactobacillus suspension and Account for U. pertusa fermentation base fluid weight 0.06% activated yeast bacterium solution be uniformly mixed and seal after, adjust fermentor in temperature be 36 DEG C, heat-preservation fermentation 36 hours, obtain the U. pertusa fermentation liquid rich in biodiasmin.This fermentation liquid filling and package is placed on 4 Obtain the U. pertusa fermented beverage rich in viable lactic acid bacteria within after-ripening 36 hours in DEG C freezer.
After measured, reach 10 by viable count of lactobacillus in U. pertusa fermented beverage made from the present embodiment6CFU/g。
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification, is all covered by the present invention.

Claims (6)

1. a kind of preparation method of the fermentation of seaweed beverage rich in viable lactic acid bacteria, it is characterised in that:The specific steps are:
(1)The preparation of edible seaweed slurry:After edible dry seaweed or new fresh seaweed clear water wash clean, rubbed with meat grinder, then plus The drinking water for entering 5-20 parts of seaweed weights impregnates to be beaten with thick pulp grinder and screened stock machine respectively after sixty minutes, obtains average grain diameter≤10 μm edible seaweed screened stock;
(2)The preparation of fermentation of seaweed base fluid:Fermentation of seaweed base fluid is deployed according to the following formulation according to parts by weight:Seaweed starches 30- 60 parts, 10-60 parts of drinking water, 5-25 parts of sugar, 5-10 parts of maltitol, 0.1-10.0 parts of food thickening agent;It will be formulated above In sugar, maltitol, food thickening agent after mixing, be added formula in water and heating for dissolving after with the sea in formula Algae slurry is uniformly mixed, and after blend compounds body mill or high pressure homogenizer homogeneous, is heated to 100 DEG C and is kept the temperature 30 minutes at this temperature, Be cooled to 30-40 DEG C it is spare;
(3)The preparation of leavening:Lactic acid bacteria culturers are aseptically activated to and expanded culture, are made after expanding culture Viable count of lactobacillus reaches 107The lactobacillus suspension of CFU/g or more;In addition 10-20 times of dry ferment is added in aroma-producing active dry yeast It in the 2.5wt% liquid glucose of weight, and is heated to 30-35 DEG C of heat preservation and activates 10 hours, obtain activated yeast bacterium solution;
(4)The preparation of fermentation of seaweed beverage:By step(2)Fermentation of seaweed base fluid obtained is added in fermentor, and seaweed hair is added The edible alcohol of ferment base fluid weight 0.1-1.0%, and the lactobacillus suspension for accounting for fermentation of seaweed base fluid weight 0.1-1% is added and accounts for seaweed After the activated yeast bacterium solution of fermentation base fluid weight 0.05-1% is uniformly mixed and seals, temperature in adjusting fermentor, heat-preservation fermentation, Obtain the fermentation of seaweed liquid rich in biodiasmin;It is small that this fermentation liquid filling and package is placed on after-ripening 18-48 in 4 DEG C of freezers When obtain the fermentation of seaweed beverage rich in viable lactic acid bacteria.
2. a kind of preparation method of fermentation of seaweed beverage rich in viable lactic acid bacteria according to claim 1, feature exist In:The edible seaweed is Rhodophyta, Phaeophyta, any one edible seaweed in Chlorophyta.
3. a kind of preparation method of fermentation of seaweed beverage rich in viable lactic acid bacteria according to claim 1, feature exist In:The lactic acid bacteria culturers are lactobacillus plantarum, bifidobacterium bifidum, bifidobacterium longum, bifidobacterium infantis, youth bifid Bacillus, bifidobacterium breve, lactobacillus bulgaricus, L. casei casei, lactobacillus acidophilus, lactobacillus reuteri, mouse Lee's sugar lactobacillus, streptococcus thermophilus it is one or more.
4. a kind of preparation method of fermentation of seaweed beverage rich in viable lactic acid bacteria according to claim 1, feature exist In:Step(2)The sugar is one of sucrose, maltose, fructose, glucose, starch syrup or a variety of.
5. a kind of preparation method of fermentation of seaweed beverage rich in viable lactic acid bacteria according to claim 1, feature exist In:Step(2)The food thickening agent be sodium alginate, xanthan gum, Arabic gum, locust bean gum, pectin, guar gum, One of gellan gum, sodium carboxymethylcellulose are a variety of.
6. a kind of preparation method of fermentation of seaweed beverage rich in viable lactic acid bacteria according to claim 1, feature exist In:Step(4)Temperature is 30-40 DEG C, heat-preservation fermentation 24-48 hours in middle adjusting fermentor.
CN201810632395.5A 2018-06-20 2018-06-20 A kind of preparation method of the fermentation of seaweed beverage rich in viable lactic acid bacteria Pending CN108902595A (en)

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CN109463750A (en) * 2018-12-15 2019-03-15 陕西微阅信息技术有限公司 A kind of preparation method of intestinal flora finishing agent
CN109965173A (en) * 2019-03-15 2019-07-05 湖州师范学院 A kind of preparation method and applications for extracting rhodophyll, polysaccharide and dietary fiber from low valued laver
CN110150651A (en) * 2019-06-11 2019-08-23 深圳保时健生物工程有限公司 It is a kind of for adjusting the preparation of infant's function of intestinal canal
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CN114395587A (en) * 2022-01-17 2022-04-26 福州迈健生物科技有限公司 Microbial fermentation production method and application of seaweed fermentation powder
CN114521647A (en) * 2022-03-23 2022-05-24 福建农林大学 Kelp probiotic fermentation product with anti-aging function and preparation method thereof
TWI819509B (en) * 2022-03-07 2023-10-21 健茂生物科技股份有限公司 Sea grape fermentation liquid manufacturing method
CN117678727A (en) * 2023-12-14 2024-03-12 山东海之宝海洋科技有限公司 Use of lactobacillus fermentation in the preparation of a flavoring
CN114521647B (en) * 2022-03-23 2024-04-26 福建农林大学 Kelp probiotics fermented product with auxiliary antioxidant capacity improving function and preparation method thereof

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CN108991318A (en) * 2018-08-08 2018-12-14 福建省农业科学院农业工程技术研究所 A kind of fermentation of seaweed beverage
CN108991318B (en) * 2018-08-08 2021-09-14 福建省农业科学院农业工程技术研究所 Seaweed fermented beverage
CN109463750A (en) * 2018-12-15 2019-03-15 陕西微阅信息技术有限公司 A kind of preparation method of intestinal flora finishing agent
CN109965173B (en) * 2019-03-15 2022-09-13 湖州师范学院 Preparation method and application of phycoerythrin, polysaccharide and dietary fiber extracted from laver in water powder
CN109965173A (en) * 2019-03-15 2019-07-05 湖州师范学院 A kind of preparation method and applications for extracting rhodophyll, polysaccharide and dietary fiber from low valued laver
CN110150651A (en) * 2019-06-11 2019-08-23 深圳保时健生物工程有限公司 It is a kind of for adjusting the preparation of infant's function of intestinal canal
CN110279023A (en) * 2019-07-06 2019-09-27 孙杨 A kind of formula and preparation method thereof of seaweed ferment gel candy
CN111772182A (en) * 2020-07-21 2020-10-16 集美大学 Seaweed ferment and preparation method thereof
CN112869142A (en) * 2021-02-07 2021-06-01 启田生物科技(大连)有限公司 Seaweed and chlorella compound enzyme and preparation method thereof
CN114395587A (en) * 2022-01-17 2022-04-26 福州迈健生物科技有限公司 Microbial fermentation production method and application of seaweed fermentation powder
TWI819509B (en) * 2022-03-07 2023-10-21 健茂生物科技股份有限公司 Sea grape fermentation liquid manufacturing method
CN114521647A (en) * 2022-03-23 2022-05-24 福建农林大学 Kelp probiotic fermentation product with anti-aging function and preparation method thereof
CN114521647B (en) * 2022-03-23 2024-04-26 福建农林大学 Kelp probiotics fermented product with auxiliary antioxidant capacity improving function and preparation method thereof
CN117678727A (en) * 2023-12-14 2024-03-12 山东海之宝海洋科技有限公司 Use of lactobacillus fermentation in the preparation of a flavoring

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