CN114806907B - Saccharomyces cerevisiae AMnb091, and separation culture method and application thereof - Google Patents
Saccharomyces cerevisiae AMnb091, and separation culture method and application thereof Download PDFInfo
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- CN114806907B CN114806907B CN202210388924.8A CN202210388924A CN114806907B CN 114806907 B CN114806907 B CN 114806907B CN 202210388924 A CN202210388924 A CN 202210388924A CN 114806907 B CN114806907 B CN 114806907B
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
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- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/60—Edible seaweed
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Abstract
The invention belongs to the field of microbial culture, and particularly relates to a culture method of saccharomyces cerevisiae AMnb091 capable of fermenting and preparing a kelp base functional product. The saccharomyces cerevisiae AMnb091 is preserved in China center for type culture Collection in 2021 at 11 months 29, and the preservation number is as follows: CCTCC NO: M20211499. The culture method comprises the following steps: s1: separating and culturing saccharomyces cerevisiae AMnb091; s2: screening saccharomyces cerevisiae AMnb091 for fermenting kelp; s3: fermenting kelp based on saccharomyces cerevisiae AMnb091 strain; s4: and (4) evaluating the fermentation effect. The saccharomyces cerevisiae AMnb091 provided by the invention can utilize natural kelp matrix to carry out rapid growth and propagation, and the yield of metabolites is increased. The kelp fermented product prepared based on the bacterial strain has excellent antioxidant and hypoglycemic activity, the alpha-glucosidase inhibition rate reaches 70.20%, and the ABTS inhibition capacity is 78.94%. Compared with the traditional culture method, the method has the advantages of high efficiency, energy conservation, time conservation, simple operation and capability of realizing production with high added value.
Description
Technical Field
The invention belongs to the technical field of microbial culture, and particularly relates to saccharomyces cerevisiae AMnb091 and a culture method of the saccharomyces cerevisiae AMnb091.
Background
Kelp, which is rich in chemical components and bioactive substances, has been used in many industrial fields such as food, feed and prodrugs, and is known as "natural health food". The kelp contains rich brown algae polysaccharide, has very high value in the processing application of functional foods and medicines, and also contains various vitamins, mineral substances and the like. Researches prove that the kelp has various biological functions of reducing blood fat, reducing blood sugar, regulating immunity, resisting blood coagulation, resisting tumors, removing lead, detoxifying, resisting oxidation and the like. However, the kelp industry in China is still in a primary state of low added value and low benefit mainly based on traditional extensive processing and primary processed products, and the development space of the kelp industry is far from being explored. Therefore, the kelp processing and fermenting industry is innovatively developed, the kelp product with nutrition and health care values is researched and developed, the healthy Chinese strategic demands are met, and the sustainable development of the kelp industry chain is promoted.
CN111154701A discloses a bacterial strain for producing algin lyase and cellulase and its application in fermenting kelp; the application of Saccharomyces cerevisiae to the fermentation of kelp or other seaweed products is well known in the literature.
Saccharomyces cerevisiae is the earliest microorganism that could be produced on an industrial scale and is the most widely used strain of yeast species today. Although the form is simple, the metabolite is numerous and rich in various physiologically active substances, and the application of the metabolite in various fields is wide. The saccharomycete, which grows mainly in a slightly acid moist sugar-containing environment and is widely distributed in the natural world, has the advantages of fast propagation, short growth period, rich enzyme systems and vigorous metabolism, and has the potential of utilizing the nutrient components in the kelp for fermentation. The yeast fermentation can not only increase the flavor of food and improve the nutritive value of food, but also has the functions of health care and body conditioning. Meanwhile, the saccharomycetes can also generate functional active factors such as glutathione, extracellular polysaccharide and the like, so that the oxidation resistance of the organism can be improved, and the biological activities of reducing blood sugar, reducing blood fat and the like can be improved. If the saccharomyces cerevisiae with strong tolerance, rich enzyme system and vigorous metabolism can be screened, powerful support can be inevitably provided for the deep processing of the kelp.
Disclosure of Invention
In order to solve the technical problems, the invention provides the saccharomyces cerevisiae AMnb091 which can utilize natural kelp to grow rapidly, has the capability of degrading alginic acid and can improve the glucose-reducing and oxidation-resisting capability of a kelp fermentation sample; also provides a culture and separation method of the strain;
the saccharomyces cerevisiae AMnb091 provided by the invention is preserved in China center for type culture Collection in 11 months and 29 months in 2021, and the preservation unit address is as follows: wuhan, wuhan university, china. Preservation number CCTCC No: m20211499, classified and named as Saccharomyces cerevisiae AMnb091.
The nucleotide sequence of the Saccharomyces cerevisiae AMnb09116s RNA provided by the invention is shown in SEQ ID NO. 1.
After the saccharomyces cerevisiae AMnb091 for fermenting and degrading the kelp is cultured in an YPD culture medium (yeast extract peptone glucose) at 30 ℃ for 24 hours, the culture medium presents white single colony, the surface is wet and glossy, the picking is easy, the edge is smooth, the diameter is 2-3mm, the colony texture is uniform, and the colors of the front side, the back side, the edge and the central part are uniform; the thallus cells are circular or elliptical, and the diameter is 5-10 μm. Glucose, maltose, fructose, trehalose and lactose can be utilized, and xylose cannot be utilized; the test of nitrate reduction and generation of amyloid is negative; ethanol and citric acid can be assimilated in a carbon source assimilation experiment, and nitrate cannot be assimilated in a nitrogen source assimilation experiment. The diazo blue B test and the urease test are both negative.
The method for separating and culturing the saccharomyces cerevisiae AMnb091 for fermenting the kelp comprises the following steps:
s1: separation and culture of saccharomyces cerevisiae
When the saccharomyces cerevisiae is separated and cultured, the method comprises the following specific steps:
(1) Taking pericarp, grinding, and placing into a test tube filled with normal saline to fully and uniformly mix the samples;
(2) Sucking the mixed sample, adding the sample into a test tube containing normal saline, and sequentially preparing suspensions with different concentrations;
(3) Sufficiently sucking mixed liquid with different concentrations from the test tube in the step (2), respectively diluting and coating the mixed liquid into a sterilized solid YPD seed culture medium, and culturing;
(4) Observing the colony morphology after the culture is finished, and selecting a milky white smooth and wet single colony for microscopic examination;
(5) Carrying out separation and purification culture on the bacterial colony which is oval in microscopic examination, checking whether mixed bacteria exist after the bacterial colony grows well, and carrying out separation and purification again if the mixed bacteria exist, until a pure bacterial strain culture is obtained;
preferably, the method comprises the following steps:
s1: separation and culture of saccharomyces cerevisiae
When the saccharomyces cerevisiae is separated and cultured, the method comprises the following specific steps:
(1) Taking 1g of fruit peel, grinding the fruit peel by using a mortar, putting the fruit peel into a test tube filled with 9mL of physiological saline, and fully and evenly mixing the samples by fully and whirling the fruit peel by using a whirling oscillator;
(2) Fully sucking 1mL of the mixed sample by using a pipette, adding the sample into a test tube containing 9mL of physiological saline, and preparing 10-2, 10-3, 10-4, 10-5 and 10-6 suspensions by analogy;
(3) Sufficiently sucking 0.1mL of the extract from a test tube with the concentration of 10-2, 10-4 and 10-6 by using a pipette, respectively diluting and coating the extract on a sterilized solid YPD seed culture medium, and culturing for 24h at 30 ℃;
(4) Observing the morphology of the bacterial colony after the culture is finished, and selecting a milky, smooth and moist bacterial colony for microscopic examination;
(5) Carrying out separation and purification culture on the bacterial colony which is oval in microscopic examination, checking whether mixed bacteria exist or not after the bacterial colony grows well, and carrying out separation and purification again if the mixed bacteria exist, until a pure bacterial strain culture is obtained;
(6) Carrying out 16S rDNA sequencing analysis on the bacterial colonies meeting the conditions, and comparing sequencing results by using BLAST to obtain saccharomyces cerevisiae;
s2: screening of Saccharomyces cerevisiae for fermenting kelp
When screening saccharomyces cerevisiae for fermenting kelp, the method comprises the following specific steps: inoculating a single colony on the YPD plate into a liquid YPD seed culture medium, culturing for 24h at 30 ℃, then inoculating into a screened kelp enzymolysis liquid culture medium according to the inoculation amount of 5-10%, and culturing for 40-60h at 30 ℃;
s3: fermented kelp
When the kelp is fermented, the specific operation steps are as follows: inoculating a single colony on the YPD plate into a liquid YPD seed culture medium, culturing for 24h at 30 ℃, then inoculating into kelp enzymolysis liquid according to the inoculum size of 5-10%, and culturing for 48h at 30 ℃; the inoculated strain is the strain screened from S2; the specific culture method comprises the following steps: supplementing glucose to the kelp enzymatic hydrolysate to 3%, sterilizing at 121 deg.C for 20min, inoculating 3% Saccharomyces cerevisiae, and shake culturing at 30 deg.C and 2000 r/min for 48 hr.
The solid/liquid YPD seed culture media described above all contained: 10g/L of yeast extract, 20g/L of peptone and 20g/L of glucose.
S4 evaluation of fermentation Effect
And (3) carrying out in-vitro blood sugar reduction and oxidation resistance evaluation on a fermentation sample of saccharomyces cerevisiae fermented kelp.
The above S1: the culture medium for separating and culturing the saccharomyces cerevisiae comprises the following components: 10g/L of yeast extract, 20g/L of peptone and 20g/L of glucose.
In the S2, the screening culture medium of the saccharomyces cerevisiae is kelp which is washed, dried and ground. The powder was passed through a 40 mesh sieve, collected, and then dissolved in distilled water at a ratio of 1; the pH was adjusted to 4.8 and the cellulase and pectinase were added at 2.5wt% and 0.26wt%, respectively. The reaction solution was stirred at 50 ℃ for 2 hours, pH was adjusted to 8.0, alkaline pectinase (0.3 wt%) was added, incubation was further performed at 60 ℃ for 1.5 hours, and finally, the reaction product was centrifuged, and the supernatant was collected as a laminarinase solution.
When kelp is fermented in the step S3: the specific operation method of the fermented kelp comprises the following steps: supplementing glucose to the kelp enzyme solution to 3%, sterilizing at 121 deg.C for 20min, inoculating 3% Saccharomyces cerevisiae, and shake culturing at 30 deg.C and 200 r/min for 48 hr.
The application of the saccharomyces cerevisiae in fermenting seaweed products and degrading alginic acid is also the content to be protected by the invention, and in the steps, the specific step of fermenting the kelp is shown as S3.
The invention has the beneficial effects that:
(1) The saccharomyces cerevisiae provided by the invention can utilize natural kelp to grow rapidly;
(2) The saccharomyces cerevisiae provided by the invention has the capability of degrading alginic acid and can improve the capabilities of reducing blood sugar and resisting oxidation of a kelp fermentation sample. The inhibition capacity to alpha-glucosidase reaches 70.20%, and the inhibition rate to ABTS is 78.94%;
(3) Compared with the traditional method, the saccharomyces cerevisiae fermentation degraded kelp provided by the invention has the advantages of high efficiency, energy conservation, time conservation, simple operation and capability of realizing high value-added production.
Drawings
FIG. 1 is a photograph showing the colony morphology of Saccharomyces cerevisiae;
FIG. 2 is a photograph showing the microscopic morphology of Saccharomyces cerevisiae.
Detailed Description
The present invention will now be further described with reference to specific embodiments in order to enable those skilled in the art to better understand the present invention.
The saccharomyces cerevisiae is preserved in China center for type culture Collection in 2021, 11 months and 29 days, and the preservation number is CCTCC No: m20211499.
Saccharomyces cerevisiae
After culturing in YPD medium at 30 deg.C for 24 hr, the medium presents white single colony, has wet and glossy surface, easy picking, smooth edge, diameter of 2-3mm, uniform colony texture, and uniform color on front and back, edge and central part; the cells are circular or oval and have a diameter of 5-10 μm. Glucose, maltose, fructose, trehalose and lactose can be utilized, xylose cannot be utilized; the test of nitrate reduction and generation of amyloid is negative; ethanol and citric acid can be assimilated in a carbon source assimilation experiment, and nitrate cannot be assimilated in a nitrogen source assimilation experiment. The diazo blue B test and the urease test are both negative.
Example 1
S1: separation and culture of Saccharomyces cerevisiae
When the saccharomyces cerevisiae is separated and cultured, the method comprises the following specific steps:
(1) Taking 1g of fruit peel, grinding the fruit peel by using a mortar, putting the fruit peel into a test tube filled with 9mL of normal saline, and fully and evenly mixing the samples by fully and whirling the fruit peel by using a whirling oscillator;
(2) Fully sucking 1mL of the mixed sample by using a pipette, adding the sample into a test tube containing 9mL of physiological saline, and repeating the steps to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 The suspension of (a);
(3) Using a pipette with a concentration of 10 -2 ，10 -4 ，10 -6 0.1mL of the mixture was sufficiently aspirated into the test tube, diluted and spread on sterilized solid YPD seed medium (10 g/L yeast extract, 20g/L peptone, 20g/L glucose) respectively, and cultured at 30 ℃ for 24 hours;
the method comprises the following specific steps: after the inoculating loop is burned and cooled by an alcohol lamp, a loop bacterial colony is picked, and a flat plate is placed beside the alcohol lamp to draw 3-4 continuous straight lines according to Z by the inoculating loop. Then the burning ring is cooled, the rotating plate is scribed in another area from the end of the first scribing area according to the same method, and 3-4 areas are scribed. After the scribing is finished, inversely culturing for 24 hours in a constant-temperature incubator at 30 ℃;
(4) Observing the colony morphology after the culture is finished, and selecting a milky white smooth and wet single colony for microscopic examination;
(5) Carrying out separation and purification culture on the bacterial colony which is oval in microscopic examination, checking whether mixed bacteria exist after the bacterial colony grows well, and carrying out separation and purification again if the mixed bacteria exist, until a pure bacterial strain culture is obtained;
(6) Carrying out 16S rDNA sequencing analysis on the bacterial colonies meeting the conditions, and comparing sequencing results by using BLAST to obtain saccharomyces cerevisiae;
s2: screening of Saccharomyces cerevisiae for fermenting kelp
Inoculating a single colony on a solid plate into a liquid YPD seed culture medium, culturing for 24h at 30 ℃, then inoculating into a screened culture medium (kelp enzymolysis liquid) according to the inoculum size of 5-10%, and culturing for 40-60h at 30 ℃;
the kelp enzymolysis liquid is obtained by the following steps: washing, drying and grinding the kelp, sieving the powder with a 40-mesh sieve, collecting, dissolving the powder in distilled water according to the proportion of 1; stirring for enzymolysis at 50 deg.C for 2 hr, adjusting pH to 8.0, adding 0.3wt% alkaline pectinase, and incubating at 60 deg.C for 1.5 hr; and finally, centrifuging the reaction product, and collecting the enzymolysis supernatant to obtain the kelp enzymolysis liquid.
Example 2
The preservation method of the saccharomyces cerevisiae comprises the following steps:
inoculating Saccharomyces cerevisiae in liquid culture medium, culturing to logarithmic phase, and sterilizing 80% glycerol with high pressure steam. 1mL of sterilized glycerol is put into the bacteria liquid and is fully and uniformly mixed to ensure that the concentration of the glycerol is about 10 to 30 percent, and the mixture is frozen and stored at the temperature of minus 80 ℃.
The liquid culture medium of the preservation method comprises the following components: 10g/L of yeast extract, 20g/L of peptone and 20g/L of glucose.
Example 3
The saccharomyces cerevisiae purification method comprises the following steps:
and (3) selecting and separating a single colony by using an inoculating loop burned by alcohol on a super clean bench, inoculating the single colony to a saccharomyces cerevisiae solid culture medium plate, and culturing at constant temperature of 30 ℃ for 24h to stop culturing. And (4) checking whether the mixed bacteria exist or not by observing the colony morphology, performing microscopic examination and the like, and continuously picking out a single colony for purification until a pure culture of the saccharomyces cerevisiae is obtained.
The solid culture medium of the saccharomyces cerevisiae comprises the following components: 10g/L of yeast extract, 20g/L of peptone, 20g/L of glucose and 15-20g/L of agar powder.
The saccharomyces cerevisiae obtained by the method has the following characteristics:
glucose, maltose, fructose, trehalose and lactose can be utilized, and xylose cannot be utilized; the test of nitrate reduction and generation of amyloid is negative; ethanol and citric acid can be assimilated in a carbon source assimilation experiment, and nitrate cannot be assimilated in a nitrogen source assimilation experiment. The diazo blue B test and the urease test are both negative.
Example 4
The effects of saccharomyces cerevisiae on oxidation resistance and blood sugar reduction of the fermented kelp are verified by adding saccharomyces cerevisiae into the kelp enzymolysis liquid (the preparation method is the same as that of example 1).
The specific method comprises the following steps:
inoculating the single colony on the YPD plate into a seed culture medium, culturing for 24h at 30 ℃, then inoculating into the kelp enzymolysis liquid according to the inoculation amount of 5-10%, supplementing glucose to 3% in the kelp enzymolysis liquid during inoculation, then sterilizing at 121 ℃ for 20min, then inoculating 3% of saccharomyces cerevisiae, and placing in a shaking table at 30 ℃ and 200 r/min for culturing, wherein the fermentation time is 48h. The preparation method of the kelp enzymolysis liquid is the same as that of example 1, and details are not repeated here.
The evaluation of the fermentation effect comprises the following steps of in-vitro blood sugar reduction and in-vitro oxidation resistance:
the in vitro blood sugar reducing experiment comprises the following steps: inhibition experiments on alpha-glucosidase. Respectively dissolving fermented kelp samples in PBS (pH 6.8), then diluting the samples into different concentrations, respectively adding 0.2U/mL alpha-glucosidase solution, preheating the test tube in a water bath kettle at 37 ℃ for 40min, adding PNPG substrate into the test tube after preheating is finished to react, wherein the reaction temperature is 37 ℃, the reaction time is 20min, and finally adding 1mol/L sodium carbonate solution with 3 times of liquid volume into the test tube to terminate the reaction. The alpha-glucosidase inhibition rate was determined.
The in vitro antioxidant experiment is as follows: the crude was purified by mixing ABTS stock solution (7 mM) and potassium persulfate solution (4.9 mM) at a concentration of 1:1 to prepare a working solution. The mixture was incubated overnight at room temperature in the dark. Thereafter, the mixture was diluted with distilled water, and the absorbance at 734nm was 0.7. + -. 0.02. The fermented kelp samples were mixed with the ABTS working solution, left for 8 minutes at room temperature in the dark and then the absorbance was recorded at 734nm with a spectrophotometer. Blanks and ascorbic acid positive controls were juxtaposed.
The method comprises the following specific steps:
supplementing glucose to the kelp enzyme solution to 3%, sterilizing at 121 deg.C for 20min, inoculating 3% Saccharomyces cerevisiae, and shake culturing at 30 deg.C and 200 r/min for 48 hr. The removal efficiency of the fermentation sample on ABTS can reach 78.63 percent respectively, and the inhibition capacity on alpha-glucosidase can reach 70.40 percent respectively.
The result shows that the oxidation resistance and the blood sugar reducing capability of the fermented sample are obviously improved by fermenting the kelp through the saccharomyces cerevisiae.
Example 5
The method for fermenting the kelp enzymolysis liquid is the same as the example 4, and the fermentation time is 24 hours. The ABTS removal efficiency of the fermentation sample can reach 72.40 percent respectively, and the alpha-glucosidase inhibition capacity can reach 75.14 percent.
Example 6
The method for fermenting the kelp enzymolysis liquid is the same as that in example 4, the fermentation time is 72 hours, the ABTS removal efficiency of the fermentation sample can reach 71.21 percent respectively, and the alpha-glucosidase inhibition capacity can reach 73.25 percent respectively.
Example 7
After the saccharomyces cerevisiae of the present invention is obtained, the inventor compares the effect of the saccharomyces cerevisiae and other strains on fermenting the kelp, the fermentation conditions are the same as those of example 5, the fermentation time is 48 hours, and the specific steps are as follows:
the results show that the ABTS removal efficiency of the Trichoderma viride sample, the Rhizopus oryzae sample, the Mucor sample and the kelp sample fermented by the Bacillus subtilis sample is respectively 38.12 percent, 39.15 percent, 42.30 percent and 67.41 percent, and the alpha-glucosidase inhibition capacity is respectively 14.59 percent, 32.15 percent, 16.26 percent and 54.10 percent. The result shows that when the saccharomyces cerevisiae is applied to kelp fermentation, the obtained fermentation product is far superior to other strains in ABTS removal efficiency and alpha-glucosidase inhibition capacity.
Sequence listing
<110> university of Qilu Industrial science
Shandong Xiao firefly Biotech Co., ltd
Shandong zhuoran Biotechnology Co.,Ltd.
Sheng, yangrong Biotechnology (Shandong) Ltd
SHANDONG CHENZHANG BIOTECHNOLOGY Co.,Ltd.
<120> saccharomyces cerevisiae AMnb091, separation culture method and application
<141> 2022-04-13
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 639
<212> DNA
<213> Saccharomyces cerevisiae AMnb091 (Saccharomyces cerevisiae AMnb 091)
<400> 1
tatttgcata tttcacaatg cggaggaaaa taaaccaacc gggattgcct tagtaacggc 60
gagtgaagcg gcaaaagctc aaatttgaaa tctggtacct tcggtgcccg agttgtaatt 120
tggagagggc aactttgggg ccgttccttg tctatgttcc ttggaacagg acgtcataga 180
gggtgagaat cccgtgtggc gaggagtgcg gttctttgta aagtgccttc gaagagtcga 240
gttgtttggg aatgcagctc taagtgggtg gtaaattcca tctaaagcta aatattggcg 300
agagaccgat agcgaacaag tacagtgatg gaaagatgaa aagaactttg aaaagagagt 360
gaaaaagtac gtgaaattgt tgaaagggaa gggcatttga tcagacatgg tgttttgtgc 420
cctctgctcc ttgtgggtag gggaatctcg catttcactg ggccagcatc agttttggtg 480
gcaggataaa tccataggaa tgtagcttgc ctcggtaagt attatagcct gtgggaatac 540
tgccagctgg gactgaggac tgcgacgtaa gtcaaggatg ctggcataat ggttatatgc 600
cgcccgtctt gacaaccgga ccaagggaat gcaaaaaag 639
Claims (3)
1. The saccharomyces cerevisiae AMnb091 is preserved in the China center for type culture Collection in 2021 at 11 months and 29 days, and the preservation number is CCTCC No: m20211499, classified and named Saccharomyces cerevisiae AMnb091.
2. The use of the saccharomyces cerevisiae AMnb091 according to claim 1 for fermenting seaweed products, degrading alginic acid.
3. Use according to claim 2, wherein the fermented seaweed product is kelp, and wherein, when kelp is fermented: inoculating the single colony screened on the YPD plate into a seed culture medium, culturing for 24h at 30 ℃, then inoculating into the kelp enzymolysis liquid according to the inoculation amount of 5-10%, supplementing glucose to 3% in the kelp enzymolysis liquid during inoculation, then sterilizing for 20min at 121 ℃, then inoculating 3% of saccharomyces cerevisiae, and placing in a shaking table at 30 ℃ and 200 r/min for culturing, wherein the fermentation time is 48h.
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|---|---|---|---|---|
| KR20100027621A (en) * | 2008-09-03 | 2010-03-11 | (주)마린바이오프로세스 | Method for preparing functional natural fermentation condiment and sea-tangle fermentation powder using fermentation of sea-tangle by yeast |
| CN107811232A (en) * | 2017-10-20 | 2018-03-20 | 福建省农业科学院农业工程技术研究所 | One main laminaria complex microorganism fishy-removing-method |
| CN109112073A (en) * | 2018-08-08 | 2019-01-01 | 福建省农业科学院农业工程技术研究所 | A kind of fermentation of seaweed algae cake |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100027621A (en) * | 2008-09-03 | 2010-03-11 | (주)마린바이오프로세스 | Method for preparing functional natural fermentation condiment and sea-tangle fermentation powder using fermentation of sea-tangle by yeast |
| CN107811232A (en) * | 2017-10-20 | 2018-03-20 | 福建省农业科学院农业工程技术研究所 | One main laminaria complex microorganism fishy-removing-method |
| CN109112073A (en) * | 2018-08-08 | 2019-01-01 | 福建省农业科学院农业工程技术研究所 | A kind of fermentation of seaweed algae cake |
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