CN107802512B - Vitamin E nanoemulsion, nano cream and preparation method thereof - Google Patents
Vitamin E nanoemulsion, nano cream and preparation method thereof Download PDFInfo
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Abstract
The invention provides a vitamin E nano-emulsion, a nano-emulsifiable paste and a preparation method thereof. The vitamin E nano-emulsion is composed of vitamin E, an oil phase, a main surfactant, a cosurfactant and water, has an average particle size of 30-60 nm, is an O/W type emulsion, is transparent or semitransparent in appearance, is low in viscosity, has good fluidity and is a uniform system with blue opalescence. The preparation method of the vitamin E nanoemulsion comprises the following steps: mixing the oil phase and vitamin E, and stirring; sequentially adding the main surfactant and the cosurfactant, and uniformly stirring; slowly dropwise adding distilled water, and stirring while adding water to obtain the final product. The vitamin E nano cream is prepared from the vitamin E nano emulsion and an O/W type cream substrate, and the prepared nano cream is white, fine, glossy and good in viscosity. The preparation method has the advantages of simple required instruments and operation steps, good stability of the prepared nano emulsifiable paste, strong permeability and good application prospect in the cosmetic direction.
Description
Technical Field
The invention belongs to the field of daily chemical products and auxiliary agents thereof, and particularly relates to a vitamin E nanoemulsion, a nano cream and a preparation method thereof.
Background
In the 21 st century, nanotechnology enjoys reputation as one of the most promising areas of development. The american national science and technology committee pointed out in 2000: about 50% of the drugs in the market will be sold worldwide in the next 10 years by nanotechnology. With the continuous and deep scientific research, novel cosmetics are emerging, and the application of nanotechnology in the daily chemical field is gradually increased.
Vitamin E, also known as tocopherol, is a derivative of chroman, a fat-soluble vitamin, which is insoluble in water, has a lipid-water partition coefficient Log P of 10, and is a significant source of interaction with the skin. Vitamin E is used as an antioxidant, can protect skin from oxidative damage, such as skin damage caused by UVA and UVB ultraviolet rays, has the effects of locking water for the skin, fading color spots, reducing skin wrinkles and the like, and makes great contribution to the health and beauty of human beings. However, vitamin E is easily oxidized to form tocopherolquinone by the influence of oxygen in the environment, and is easily decomposed by irradiation of ultraviolet rays, and a normal vitamin E emulsion having a large particle size is hardly absorbed by epidermal cells. These problems can be solved by preparing vitamin E into O/W type nanoemulsion. In the preparation method of vitamin E nanoemulsion, a high-energy emulsification method is currently dominant, but the required equipment is complex and requires higher energy input, high-speed stirring and high-pressure environment are generally required, the preparation process is complex, colostrum is firstly required to be prepared, and then refined emulsion is further prepared, so that the wide application of the colostrum in industrial production is limited.
The self-emulsifying method is an energy-saving emulsion preparation method which is simple, convenient and quick to operate, environment-friendly and free of special instruments and equipment and high-energy input. The preparation of the nano-emulsion by the self-emulsifying method usually requires four components of a surfactant, a cosurfactant, oil and water, and the obtained nano-emulsion is transparent or semitransparent liquid with the particle size of 1-100 nm. When the nano-emulsion is prepared by using the self-emulsification method, the components of the oil phase have important influences on the self-emulsification of the nano-emulsion and the properties of the emulsion, and the rate of the self-emulsification is maximized when the miscibility of the oil phase and the water phase is good. The HLB value of the surfactant, the proportion of each component, the miscibility of the oil phase and the water phase and the like are important influencing factors for preparing the nano-emulsion by the automatic emulsification method.
Compared with cosmetics produced by the traditional process, the cosmetics produced by adopting the nanotechnology can better exert the beautifying and skin-care effects of the active ingredients. The vitamin E cream is a common cosmetic and has the skin care functions of preventing skin aging, improving dry skin, enabling the skin to retain moisture, fading color spots, reducing skin wrinkles and the like. At present, the commercially available vitamin E cream is mostly prepared by adopting the traditional process, namely normal vitamin E is added into a cream matrix, and the common addition amount is 0.5-5%.
Disclosure of Invention
The invention provides a vitamin E nano-emulsion, a nano-emulsifiable paste and a preparation method thereof, aiming at solving the following technical problems: (1) the preparation method overcomes the defects of the traditional vitamin E nanoemulsion preparation method, adopts self-emulsification to prepare the vitamin E nanoemulsion, simplifies preparation equipment and process through formula optimization, and is convenient to apply in industrial production; (2) the vitamin E nano-emulsion is prepared into the vitamin E nano-emulsifiable paste, so that the problem of poor absorption effect of the traditional vitamin E emulsifiable paste is solved; (3) the particle size of the vitamin E nanoemulsion is controlled in a narrow range, so that the stability and the permeability of the nanoemulsion are improved.
The invention relates to a self-emulsifying method for preparing vitamin E nano-emulsion and emulsifiable paste. The self-emulsifying method has the difficulty that stable and uniform nano emulsion is obtained under conventional stirring through selection and proportioning of an oil phase and a surfactant. The inventors screened oil phases using a controlled variation method, in which neither soybean oil nor coconut oil could form a nanoemulsion, but only isopropyl myristate could form a nanoemulsion, for reasons that may be related to the smaller molecular chain of isopropyl myristate, since the longer the molecular chain of oil phase, the less likely it was to embed a surfactant, while the smaller molecular oil phase could be embedded into a surfactant as easily as a co-surfactant to form a nanoemulsion. Meanwhile, isopropyl myristate has good permeability and moisturizing performance, and can improve the permeability and moisturizing performance of the prepared nano-emulsion and emulsifiable paste.
When the inventor selects the cosurfactant, earlier experiments show that when glycerin with smaller molecular weight is used as the cosurfactant in the research, the glycerin has more obvious influence on the particle size of the nanoemulsion, the particle size of the nanoemulsion is firstly reduced, then basically kept unchanged and finally slightly increased along with the gradual increase of the addition amount of the glycerin, and the particle size can be reduced to the minimum in the same degree when the dosage ratio of the glycerin to the compound surfactant is 30% and 60%. Therefore, when glycerin is selected as the cosurfactant, on one hand, the moisturizing performance of the prepared product can be improved, on the other hand, the particle size of the product can be effectively controlled in a smaller range, and the improvement of the transdermal capacity and the permeability of the product is facilitated, so that the absorption rate of vitamin E is improved.
The technical scheme provided by the invention is as follows:
a vitamin E nanoemulsion comprises a nanoemulsion matrix and vitamin E.
Wherein the nanoemulsion matrix consists of the following components in percentage by mass: 11-38% of oil phase, 25-45% of surfactant and the balance of water. The mass of the vitamin E accounts for 3-9% of that of the nano-emulsion matrix.
The vitamin E nanoemulsion is an O/W type vitamin E nanoemulsion, and the particle size distribution range of the vitamin E nanoemulsion is 30-60 nm.
The oil phase is isopropyl myristate.
The surfactant comprises a main surfactant and a cosurfactant, wherein the mass ratio of the main surfactant to the cosurfactant is 1: 0.3-0.6.
The main surfactant is a compound surfactant and is prepared by compounding Tween 80 and span 20, the mass ratio of the Tween 80 to the span 20 is 1-3: 1, and the HLB range is 11.8-13.4.
The cosurfactant is glycerol.
A preparation method of the vitamin E nanoemulsion comprises the following steps:
(1) mixing the oil phase and vitamin E, and stirring;
(2) sequentially adding the main surfactant and the cosurfactant, and uniformly stirring;
(3) dropwise adding distilled water while stirring until obtaining transparent or semitransparent nanoemulsion showing blue opalescence.
A method for preparing vitamin E nano cream by using the vitamin E nano cream comprises the following steps:
(1) precisely weighing O/W type cream matrix, and heating at constant temperature of 60 deg.C to completely melt;
(2) slightly cooling, adding vitamin E nanoemulsion, stirring uniformly, and cooling to room temperature to obtain the final product.
The preparation method of each 1000g O/W type cream base comprises the following steps:
89.7g of stearic acid, 106.0g of glycerol, 150.75g of liquid paraffin, 52.8g of glycerin monostearate, 53.0g of white vaseline, 2mL of triethanolamine, 2g of sodium dodecyl sulfate and 1g of ethylparaben are mixed, heated and melted at the constant temperature of 60 ℃ and stirred uniformly, and water is added to 1000g while stirring.
According to the method for preparing the vitamin E nano-emulsifiable paste, the proportion range of the O/W-type emulsifiable paste matrix to the vitamin E nano-emulsifiable paste is 2-8: 1.
The invention has the following beneficial effects:
(1) the vitamin E nanoemulsion has small particle size and uniform distribution, the encapsulation rate reaches more than 85 percent, the transdermal permeability is obviously superior to that of the vitamin E, and the stability of the vitamin E is improved;
(2) the vitamin E nano cream has good stability, superior percutaneous permeability compared with the vitamin E cream and no irritation to skin;
(3) through the optimization of the formula, the preparation process of the nano-emulsion is simple, the requirements on instruments and reaction conditions are low, and the environment is friendly;
(4) the glycerol is used as the cosurfactant, so that the particle size of the prepared vitamin E nanoemulsion can be effectively controlled to be 30-60 nm, the distribution range is narrow, and the permeability of the vitamin E is remarkably improved;
(5) the vitamin E is encapsulated in the O/W type nano-milk, so that the vitamin E has better antioxidant performance.
Drawings
FIG. 1 is a relationship curve of vitamin E nanoemulsion particle size and drug loading;
FIG. 2 is a graph showing the relationship between the particle size of vitamin E nanoemulsion at 4 ℃ and the storage time;
FIG. 3 is a graph of in vitro transdermal permeation of 6% (w/w) vitamin E nanoemulsion and vitamin E, Q-cumulative permeation;
FIG. 4 is a micrograph of vitamin E nanocream;
FIG. 5 is a graph of the in vitro release of vitamin E nanocream and vitamin E cream;
fig. 6 shows the in vitro transdermal penetration of vitamin nano-cream and vitamin E cream.
Detailed Description
The following examples are given for the purpose of illustrating the present invention, but the scope of the present invention is not limited to the following examples.
The following percentages are mass fractions.
EXAMPLE 13 vitamin E nanoemulsion preparation of wt% vitamin E
The vitamin E nanoemulsion comprises the following raw materials: 0.0339g vitamin E (medicinal grade, purity is more than or equal to 98.8%, the same below), 0.1560g isopropyl myristate, 0.1050g span 20, 0.1750g Tween 80, 0.0840g glycerol and 0.6100g water.
Wherein the nanoemulsion matrix: 14% of oil phase, 32% of surfactant and the balance of water. The mass of the vitamin E accounts for 3 percent of the mass of the nano-emulsion matrix. The mass ratio of the main surfactant (span 20 and tween 80) to the co-surfactant (glycerin) is 1:0.3, the HLB value of the main surfactant is 12.6, and the mass ratio of tween 80 to span 20 is 1.67: 1.
The preparation method comprises the following steps: firstly, adding an oil phase (isopropyl myristate) and vitamin E which are precisely weighed to required amounts, mixing and uniformly stirring; sequentially adding main surfactant (span and tween) and cosurfactant (glycerol), and stirring; and then slowly dropwise adding distilled water, adding water while stirring, wherein the system is uniform and good in fluidity at the beginning, gradually becomes viscous and gel-like along with the increase of the amount of the added water, continuously dropwise adding the distilled water, suddenly becomes thin and transparent or semitransparent, and is accompanied by blue opalescence, and then dropwise adding the water to the required amount to obtain the vitamin E nanoemulsion.
EXAMPLE 26 wt% vitamin E nanoemulsion preparation
The vitamin E nanoemulsion comprises the following raw materials: 0.0678g vitamin E, 0.1560g isopropyl myristate, 0.1050g span 20, 0.1750g Tween 80, 0.0840g glycerol, 0.6100g water.
Wherein the nanoemulsion matrix: 14% of oil phase, 32% of surfactant and the balance of water. The mass of the vitamin E accounts for 6 percent of the mass of the nano-emulsion matrix. The mass ratio of the main surfactant (span 20 and tween 80) to the co-surfactant (glycerin) is 1:0.3, the HLB value of the main surfactant is 12.6, and the mass ratio of tween 80 to span 20 is 1.67: 1.
The preparation method is the same as example 1.
EXAMPLE 39 vitamin E nanoemulsion of wt% vitamin E preparation
The vitamin E nanoemulsion comprises the following raw materials: 0.1017g of vitamin E, 0.1560g of isopropyl myristate, 0.1050g of span 20, 0.1750g of Tween 80, 0.0840g of glycerol and 0.6100g of water.
Wherein the nanoemulsion matrix: 14% of oil phase, 32% of surfactant and the balance of water. The mass of the vitamin E accounts for 9 percent of the mass of the nano-emulsion matrix. The mass ratio of the main surfactant (span 20 and tween 80) to the co-surfactant (glycerin) is 1:0.3, the HLB value of the main surfactant is 12.6, and the mass ratio of tween 80 to span 20 is 1.67: 1.
The preparation method is the same as example 1.
EXAMPLE 43 wt% vitamin E nanoemulsion preparation
The vitamin E nanoemulsion comprises the following raw materials: 0.0279g vitamin E, 0.1021g isopropyl myristate, 0.0653g span 20, 0.1959g Tween 80, 0.1567g glycerol and 0.4086g water.
Wherein the nanoemulsion matrix: 11% of oil phase, 45% of surfactant and the balance of water. The mass of the vitamin E accounts for 3 percent of the mass of the nano-emulsion matrix. The mass ratio of the main surfactant to the cosurfactant is 1:0.6, the HLB value of the main surfactant is 13.4, and the mass ratio of the Tween 80 to the span 20 is 3: 1.
The preparation method is the same as example 1.
EXAMPLE 53 preparation of vitamin E nanoemulsion of wt% vitamin E
The vitamin E nanoemulsion comprises the following raw materials: 0.0248g vitamin E, 0.3136g isopropyl myristate, 0.0794g span 20, 0.0794g Tween 80, 0.0476g glycerol and 0.3054g water.
Wherein the nanoemulsion matrix: oil phase 38%, surfactant 25%, and water in balance. The mass of the vitamin E accounts for 3 percent of the mass of the nano-emulsion matrix. The mass ratio of the main surfactant to the co-surfactant is 1:0.3, the HLB value of the main surfactant is 11.8, and the mass ratio of the Tween 80 to the span 20 is 1: 1.
The preparation method is the same as example 1.
EXAMPLE 63 preparation of vitamin E nanoemulsion of wt% vitamin E
The vitamin E nanoemulsion comprises the following raw materials: 0.0248g vitamin E, 0.3136g isopropyl myristate, 0.0694g span 20, 0.0694g Tween 80, 0.0676g glycerol and 0.3054g water.
Wherein the nanoemulsion matrix: oil phase 38%, surfactant 25%, and water in balance. The mass of the vitamin E accounts for 3 percent of the mass of the nano-emulsion matrix. The mass ratio of the main surfactant to the co-surfactant is 1:0.5, the HLB value of the main surfactant is 11.8, and the mass ratio of the Tween 80 to the span 20 is 1: 1.
The preparation method is the same as example 1.
Example 7 analysis of the Performance of vitamin E nanoemulsion
(1) The pH values of the vitamin E nanoemulsion prepared in examples 1 to 3 were tested to be 6.54 to 6.69, which are within the range of the pH value of the normal skin surface, and the skin was in the optimum state for absorbing nutrients.
(2) The encapsulation efficiency of the vitamin E nanoemulsion prepared in the examples 1 to 3 is tested, and the encapsulation efficiency reaches over 85 percent, so that the encapsulation effect is good.
(3) The particle size of the vitamin E nanoemulsion prepared in the test examples 1 to 3 is in the range of 30 to 60nm, and the particle size of the nanoemulsion increases with the increase of the drug loading amount of the vitamin E, as shown in FIG. 1.
(4) Stability of vitamin E nanoemulsion prepared in examples 1 to 3
Test method 1: centrifuging at 4000r/min for 15min to observe whether the emulsion is layered. Test results show that the nano-emulsions prepared in examples 1-3 are not layered and have good centrifugal stability.
The test method 2: firstly, detecting the initial particle size of the freshly prepared nano-emulsion and the initial concentration of vitamin E in the freshly prepared nano-emulsion, then storing the nano-emulsion at 4 ℃, detecting the particle size of the nano-emulsion and the concentration of the vitamin E in the nano-emulsion at intervals, and then comparing the initial particle size and the concentration with the particle size and the concentration of the nano-emulsion stored for 20 days or the particle size and the concentration of the nano-emulsion in the nano-emulsion before sedimentation and layering.
Fig. 2 shows a graph showing the change in particle size, and it can be seen that the initial particle size was substantially maintained as compared with the particle size after storage at 4 c for 20 days. The change of the vitamin E concentration in the nanoemulsion is shown in the table 1, and the concentration of the vitamin E nanoemulsion in the previous day or 20 th day of storage before the phenomena of sedimentation, layering and the like is basically kept unchanged compared with the initial concentration. The stability of the vitamin E nanoemulsion is good by integrating the particle size and concentration change conditions.
TABLE 1 concentration variation of vitamin E in nanoemulsion (n ═ 3)
Note: the drug loading rate refers to the mass percentage of the vitamin E added when the vitamin E nanoemulsion is prepared; ND means that the nanoemulsion was not denatured, and the time point for measuring the vitamin E concentration was day 20. The vitamin E nanoemulsion adopts gas chromatography and HP-1 capillary column (30m multiplied by 0.25mm multiplied by 0.25 mu m); carrier gas: n is a radical of2(ii) a Column temperature: 270 ℃; sample inlet temperature: 300 ℃; detector temperature: 300 ℃; a detector: a hydrogen Flame Ion Detector (FID); sample introduction amount: 1 mu L of the solution; and (3) sample introduction mode: directly feeding a sample; internal standard: n-dotriacontane.
(5) Testing of the in vitro transdermal Capacity of the vitamin E nanoemulsion prepared in example 2
FIG. 3 is a graph comparing the in vitro transdermal capacity of vitamin E nanoemulsion prepared in example 2 with that of vitamin E, and it can be seen from the graph that: the unit area cumulative permeation quantity (Q) of the vitamin E nanoemulsion at any time point is obviously higher than that of the vitamin E, and the steady transdermal rate (183.5 mu g cm) of the vitamin E nanoemulsion-2·h-1) Is obviously higher than vitamin E (65.9 mug cm)-2·h-1). The results show that the in vitro transdermal capacity of the vitamin E nanoemulsion is obviously superior to that of the vitamin E.
(6) Testing of skin irritation of vitamin E nanoemulsion prepared in example 2
The test method comprises the following steps: the nanoemulsion is applied to the skin of unhaired animals (New Zealand white rabbits) once a day for 14 days continuously, the results are observed after the nanoemulsion is applied for 1 hour, grading and stimulation intensity grading are carried out according to a method in a skin irritation test in cosmetic hygiene Specification (2007) (the integral value is less than 0.5 and is non-irritant), the average integral of each animal in each day is calculated, the skin irritation intensity is judged, and cosmetics with the skin irritation intensity of medium irritation or above are judged to be unqualified products.
The test results are as follows, with a daily score per animal of 0.357 and less than 0.5 for the vitamin E nanoemulsion, indicating that the vitamin E nanoemulsion is not irritating to the skin.
Example 81% (w/w) preparation of vitamin E Nanoprotection (mass ratio of cream base to nanoemulsion 2:1)
The vitamin E nano cream comprises the following components in parts by weight: 1g of 3% vitamin E nano-emulsion and 2g O/W type cream matrix. The cream base is prepared from the following components: 89.7g of stearic acid, 106.0g of glycerol, 150.75g of liquid paraffin, 52.8g of glyceryl monostearate, 53.0g of white vaseline, 2mL of triethanolamine, 2g of sodium dodecyl sulfate, 1g of ethylplatinum and water until the weight is 1000 g.
The preparation method of the vitamin E nano cream comprises the following steps: firstly, preparing 3% (w/w) vitamin E nanoemulsion according to the method of the embodiment 1, then precisely weighing 2g of cream substrate, heating the cream substrate in a constant-temperature water bath at 60 ℃ to completely melt the substrate, slightly cooling the cream substrate, adding 1g of prepared 3% vitamin E nanoemulsion, uniformly stirring the mixture, and cooling the mixture to room temperature to obtain the vitamin E nanoemulsion. The obtained product is a uniform cream which is white, has fine and smooth surface, luster, good viscosity, and is easy to clean and apply. The observation result under a microscope is shown in figure 4, and the vitamin E nano cream has regular shape and uniform arrangement.
Example 91% (w/w) preparation of vitamin E Nanoprotection (quality ratio of cream base to nanoemulsion 5:1)
The vitamin E nano cream comprises the following components in parts by weight: 1g of 6% vitamin E nano-emulsion and 5g O/W type cream matrix. The cream base is prepared from the following components: 89.7g of stearic acid, 106.0g of glycerol, 150.75g of liquid paraffin, 52.8g of glyceryl monostearate, 53.0g of white vaseline, 2mL of triethanolamine, 2g of sodium dodecyl sulfate, 1g of ethylplatinum and water until the weight is 1000 g.
The preparation method of the vitamin E nano cream comprises the following steps: firstly, 6% (w/w) of vitamin E nanoemulsion is prepared according to the method of the embodiment 2, then 5g of cream substrate is precisely weighed, the cream substrate is heated in a thermostatic water bath at 60 ℃ to be completely melted, after the cream substrate is slightly cooled, 1g of prepared 6% of vitamin E nanoemulsion is added, the mixture is uniformly stirred, and the mixture is cooled to the room temperature, so that the cream is obtained.
Example 101% (w/w) preparation of vitamin E Nanoprotection (cream base to nanoemulsion mass ratio 8:1)
The vitamin E nano cream comprises the following components in parts by weight: 1g of 9% vitamin E nano-emulsion and 8g O/W type cream matrix. The cream base is prepared from the following components: 89.7g of stearic acid, 106.0g of glycerol, 150.75g of liquid paraffin, 52.8g of glyceryl monostearate, 53.0g of white vaseline, 2mL of triethanolamine, 2g of sodium dodecyl sulfate, 1g of ethylplatinum and water until the weight is 1000 g.
The preparation method of the vitamin E nano cream comprises the following steps: firstly, preparing 9% (w/w) vitamin E nanoemulsion according to the method of the embodiment 3, then precisely weighing 8g of cream substrate, heating the cream substrate in a constant-temperature water bath at 60 ℃ to completely melt the substrate, slightly cooling the cream substrate, adding 1g of prepared 9% vitamin E nanoemulsion, uniformly stirring the mixture, and cooling the mixture to room temperature to obtain the vitamin E nanoemulsion.
Example 11 Performance analysis of vitamin E Nanoparticulates
(1) pH value test
The pH value of the vitamin E nano cream obtained in the embodiment 7 is 6.87-6.95, and the skin can be in the best state of absorbing nutrition within the pH value range of the surface of normal skin.
(2) Stability test
After the vitamin E nano cream obtained in the embodiment 7 is centrifuged for 15min at 4000r/min, the surface fineness, the glossiness and the smearing and cleaning conditions of the vitamin E nano cream are the same as those before centrifugation. And no bubble or oil-water separation and other abnormal conditions are generated after the centrifugation, which shows that the vitamin E nano cream has good centrifugal stability.
(3) In vitro Release behavior and in vitro transdermal Capacity test
In vitro release behavior and in vitro transdermal penetration of the vitamin E nano-cream obtained in example 7: as shown in fig. 5, the cumulative release percentage of the vitamin E nano cream is significantly higher than that of the vitamin E cream, indicating that the vitamin E nano cream has a better effect of releasing vitamin E; and the vitamin E nano cream has high release speed, and can reach a relatively balanced state after being released in a short time. The results show that: the vitamin E nano cream has a rapid and stable vitamin E release effect.
FIG. 6 shows the cumulative permeation per unit area of vitamin E nano-cream and vitamin E creamQuantity (Q) is compared to the graph. As can be seen from the figure, the cumulative permeation amount per unit area (Q) of the vitamin E nano cream is higher than that of the vitamin E cream and the vitamin E nano cream under the same permeation time (42.8 mu g cm)-2h-1) Also higher than vitamin E cream (33.4. mu.g.cm)-2h-1) The results show that the vitamin E nano cream has better transdermal capability than the vitamin E cream.
(4) Skin irritation test
Example 7 skin irritation test of vitamin E nano cream obtained for 14 days: the daily score of vitamin E cream was 0.214 and less than 0.5 per animal, indicating that vitamin E cream was not irritating to the skin.
In conclusion, the vitamin E nanoemulsion and the emulsifiable paste prepared by the invention have good stability, strong permeability and easy absorption, and have good application prospect in the cosmetic direction; the preparation method provided has the advantages of simple required instruments and equipment and simple operation steps, and can be used for industrial production.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention should be included in the scope of the present invention.
Claims (5)
1. A vitamin E nanoemulsion is characterized in that,
the vitamin E nanoemulsion is an O/W type vitamin E nanoemulsion, and the particle size distribution range of the vitamin E nanoemulsion is 30-60 nm;
comprises a nano-emulsion matrix and vitamin E;
the nano-emulsion matrix comprises the following components in percentage by mass: 11-38% of oil phase, 25-45% of surfactant and the balance of water;
the mass of the vitamin E accounts for 3-9% of that of the nano-emulsion matrix;
the oil phase is isopropyl myristate;
the surfactant comprises a main surfactant and a cosurfactant, wherein the main surfactant is compounded by tween 80 and span 20, and the cosurfactant is glycerol; the mass ratio of the main surfactant to the cosurfactant in the surfactant is 1: 0.3-0.6; the mass ratio of the Tween 80 to the span 20 in the main surfactant is 1-3: 1, and the HLB range is 11.8-13.4.
2. A method for preparing the vitamin E nanoemulsion of claim 1, comprising the steps of:
(1) mixing the oil phase and vitamin E, and stirring;
(2) sequentially adding the main surfactant and the cosurfactant, and uniformly stirring;
(3) dropwise adding distilled water while stirring until obtaining transparent or semitransparent nanoemulsion showing blue opalescence.
3. A method for preparing vitamin E nanocream using the vitamin E nanoemulsion of claim 1, comprising the steps of:
(1) accurately weighing O/W type cream matrix 60oC, heating at constant temperature to completely melt the mixture;
(2) slightly cooling, adding vitamin E nanoemulsion, stirring uniformly, and cooling to room temperature to obtain the final product.
4. The method for preparing vitamin E nanocream according to claim 3, wherein the method for preparing per 1000g O/W type cream base is as follows:
mixing 89.7g stearic acid, 106.0g glycerol, 150.75g liquid paraffin, 52.8g glyceryl monostearate, 53.0g white vaseline, 2mL triethanolamine, 2g sodium dodecyl sulfate, 1g ethylplatinum, 60goC, heating to melt and stirring uniformly, and adding water to 1000g while stirring.
5. The method of preparing vitamin E nanocream according to claim 3, characterized in that: the mass ratio of the O/W type emulsifiable paste matrix to the vitamin E nanoemulsion is 2-8: 1.
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| CN114344199A (en) * | 2022-02-26 | 2022-04-15 | 南京同仁堂健康产业有限公司 | Vitamin E milk, preparation method and application thereof, moisturizing cream and preparation method thereof |
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