CN107796772A - A kind of portable instrument and the method for detecting theanine - Google Patents
A kind of portable instrument and the method for detecting theanine Download PDFInfo
- Publication number
- CN107796772A CN107796772A CN201711106211.3A CN201711106211A CN107796772A CN 107796772 A CN107796772 A CN 107796772A CN 201711106211 A CN201711106211 A CN 201711106211A CN 107796772 A CN107796772 A CN 107796772A
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- China
- Prior art keywords
- theanine
- light source
- cooling device
- heater
- instrument
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
Abstract
A kind of portable theanine detecting instrument, including closed chamber and display module；Light source, heater, cooling device, reaction tank, Photoelectric Detection module, modular converter, control module, display module are followed successively by inside closed chamber；The heater, cooling device, reaction tank are connected by pipeline, pipeline is provided with pneumatic operated valve, air driven pump, the light source includes the visible light source for producing 570nm and produces 740nm visible light sources, according to langbobier law, complex compound realizes the quick detection to theanine concentration to the difference of the visible absorbance of specific wavelength caused by the theanine and ninhydrin reaction of various concentrations.Compared with GB/T8314, change theanine, cushioning liquid and ninhydrin ratio, it is not necessary to make blank solution sample.
Description
Technical field
The invention belongs to technical field of biological, and in particular to a kind of detecting instrument for detecting theanine and detection side
Method.
Background technology
Tealeaves is that main chemical compositions have theanine, tea more made of the young shoot tender leaf of tea tree passes through series of processes
Phenol, tea albumen, caffeine etc..Theanine (N- ethyls-γ-paddy ammonia phthalein amine) is a kind of distinctive amino acid, theanine in tealeaves
Content directly determine the quality of tealeaves.Theanine accounts for more than the 50% of free amino acid of tea, theanine content in stem tea
About 1%-2%.Theanine is not only fresh refreshing, has the function that to alleviate bitter, the increase fragrant and sweet taste of millet paste, but also have antagonism coffee
Nervous system caused by coffee alkali is excited, and the effect of hypotensive.
The assay method of theanine includes electrochemical methods, paper chromatography and thin-layered chromatography, gas chromatography, ion friendship
Colour changing spectrometry, capillary electrophoresis and high performance liquid chromatography etc..GB/T8314《The measure of tea free amino acid total amount》In adopt
With ninhydrin, heat is total to ninhydrin under conditions of pH 8.0 using a- amino acid, violet complex is formed, recycles
AAS determines its content at a particular wavelength.It generally only enters under laboratory conditions as conventional detection index
OK.For tea tree quick breeding, it is necessary on the basis of field test, using while screen, Bian Kuofan, while carry out regional testing
With the method for natomical leaf structure identification, superior type of the suitable method as black tea processed is selected, and to being selected in type strain
Phenological period, yield, quality and the resistance of system synchronize identification, and the breed breeding cycle was 10 or so years.Therefore, reflected in field
Timing, it is necessary to a kind of portable instrumentation, can in field quick detection fresh tea leaf theanine content, help kind fast
Speed screening, realizes local tea variety quick breeding.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of portable instrumentation and with the instrument quick detections
The method of theanine.
In order to solve the technical problem, the present invention devises a kind of method with portable instrument quick detection theanine,
Cleaning Principle is：According to Lambert-Beer's law, complex compound is to certain wave caused by the theanine and ninhydrin reaction of various concentrations
The difference of long visible absorbance, realize the quick detection to theanine concentration.Compared with GB/T8314, change theanine,
Cushioning liquid and ninhydrin ratio, it is not necessary to which blank solution contrasts, and is developed into portable instrument, is easy to field to detect, simplifies reality
Test the cumbersome step in room；The automatic heat temperature raising of this instrument, the mode of cooling replace boiling water bath heating, natural cooling in GB/T8314
Mode, can preferably control heating-up temperature, shorten the reaction time.
Whole instrument is by light source module, heater, cooling device, reaction tank, Photoelectric Detection module, modular converter, control
The part such as molding block, display module forms.
The light source module is made up of LED1 and LED2；LED1 produces the visible ray of 570nm specific wavelength, and LED2 is produced
The visible ray of 740nm specific wavelength.The theanine solution of various concentrations is different to 570nm visible absorbance, and right
740nm visible ray is not absorbed, and the suction of 740nm visible rays is subtracted by the absorbance of the 570nm of theanine solution visible ray
Luminosity, reach the purpose for eliminating ambient interferences.When theanine detects, 570nm visible light source and 740nm visible ray are opened
Source, while theanine sample is irradiated, the light intensity by the 570nm visible rays of theanine sample and 740nm visible ray is detected, is led to
Resolving is crossed, eliminates ambient interferences, is acted on for substituting blank solution in GB/T8314；
The heater, cooling device, reaction tank are connected by pipeline, and pipeline is provided with pneumatic operated valve, and the slurries of tealeaves pass through
Liquid level difference flows into cooling device by pipeline by heater and eventually flowed into reaction tank.In connection cooling device and reaction tank
Air driven pump is set on pipeline, and the tealeaves clear liquid after standing is transported in reaction tank by air driven pump by cooling device；
Reaction tank places tealeaves clear liquid to be tested；
The Photoelectric Detection module is used for detecting ultraviolet light intensity, is made up of photodetection pipe and signal conditioning circuit；Photodetection
Pipe converts optical signals to electric signal, and be amplified by signal conditioning circuit, filter after be converted into standard analog electric signal；
The standard analog electric signal that Photoelectric Detection module exports is converted to digital letter by the modular converter by sampling, keeping
Number；
The control module control heater, cooling device and pneumatic operated valve, the switch of air driven pump between them, and control
LED light source produces the light of specific wavelength, and the data signal of modular converter input is handled, calculate, further controlled
Display module processed.
The display module shows the data of test, analysis.
GB/T8314 National Standard Methods measure a sample and taken around 2 hours, and this method time is about dozens of minutes, contracting
Short time of measuring, the quick measure that can be applicable in tea tree seed selection and tea products production process.
It is as follows the step of theanine in detection fresh tea leaf using the portable instrument：
Step 1：Instrument is opened
（1）Switch on power, instrument zeroing；570nm visible light source and 740nm visible light source are opened, while irradiates theanine
Sample, the light intensity by the 570nm visible rays of theanine sample and 740nm visible ray is detected, by resolving, eliminate background and do
Disturb, acted on for substituting blank solution in GB/T8314；
Step 2：Fresh tea leaf sample treatment
（1）Tealeaves cleans；
（2）Tealeaves is made by slurries by beater, takes filtrate in heater；
（3）The phosphate buffers of pH 8.0 and 2% ninhydrin configured is added into filtrate, makes filtrate, the phosphoric acid of pH 8.0
Salt buffer, the ratio of 2% ninhydrin three are 1:0.5:1；
（4）Set the heat time；Heater and the valve in cooling device are then turned on, by liquid level difference by heater stream
To cooling device, the fan in cooling device is opened, standing is cooled to normal temperature, closes cooling fan；
Step 3：The detection of testing sample
（1）After tealeaves slurries stand cooling, the pneumatic pump valve opened between cooling device and reaction tank extracts supernatant liquor
Into reaction tank, system automatic start is tested tealeaves slurries；
（2）Instrument shows theanine test result.
Step 4：The cleaning of instrument
（1）Container in heater, cooling device and reaction tank are taken out successively；
（2）Flushed three times, dried in case next time uses with clear water respectively；
（3）Finally the container in heater, cooling device and reaction tank are reset into instrument.
The present invention has the following advantages that compared with prior art：
1 is compared with national standard, and heater, cooling device, reaction tank are connected by pipeline in portable instrument of the present invention,
Pipeline is provided with pneumatic operated valve, and the slurries of tealeaves flow to cooling device by liquid level difference by heater, by cooling device with electronic
Pumping gets reaction tank, realizes the rapid and handy formula detection of theanine, and does not need blank solution contrast, and National Standard Method detects
Theanine is needed in laboratory operation.
2nd, the physicochemical property of theanine and rapid detection apparatus organic assembling it is quick to can be applicable to tea tree breed by this patent
Quick measure in seed selection and tea products production process, instrument can industrialized productions.
Brief description of the drawings
Fig. 1 instrument composition schematic diagrams
No. 6 Fig. 2 theanine, black tea, green tea 1, green tea 3, green tea all band scanning figures
Embodiment
With reference to embodiment, the present invention is described in detail.The embodiment is only the preferable embodiment party to the present invention
Formula, not makees any formal limitation to the present invention, and every technical spirit according to the present invention is to embodiment of above
The simple modification made, equivalent variations and modification, are belonged in the range of technical solution of the present invention.
Fig. 1 is instrumentation diagram, is made up of closed chamber and display module, and light source module is followed successively by inside closed chamber（1）Plus
Thermal（2）, cooling device（3）, reaction tank（4）, Photoelectric Detection module（5）, modular converter（6）, control module（7）, display
Module（8）.
The light source module is made up of LED1 and LED2；LED1 produces the visible ray of 570nm specific wavelength, and LED2 is produced
The visible ray of 740nm specific wavelength.The theanine solution of various concentrations is different to 570nm visible absorbance, and right
740nm visible ray is not absorbed, and the suction of 740nm visible rays is subtracted by the absorbance of the 570nm of theanine solution visible ray
Luminosity, reach the purpose for eliminating ambient interferences.When theanine detects, 570nm visible light source and 740nm visible ray are opened
Source, while theanine sample is irradiated, the light intensity by the 570nm visible rays of theanine sample and 740nm visible ray is detected, is led to
Resolving is crossed, eliminates ambient interferences, is acted on for substituting blank solution in GB/T8314；
The Photoelectric Detection module（5）Model LSSPD-U4, for detecting visual intensity；The modular converter（6）'s
Model AD7715, for the analog signal of the output of Photoelectric Detection module is converted into data signal；The control module（7）'s
Model STC89C52RC, for the work of light source module is resolved and controlled to detection signal；The display module（8）Type
Number it is LCD1602, detection, calculation result is shown.
The heater（2）, cooling device（3）, reaction tank（4）It is connected by pipeline, pipeline is provided with pneumatic operated valve
（a）, air driven pump（b）, the slurries of tealeaves are by liquid level difference by heater（2）Flow to cooling device（3）, then by cooling device
（3）In supernatant liquor pass through air driven pump（b）It is drawn into reaction tank（4）In.Realize auto injection operation.
The step of detecting theanine using the instrument is as follows：
Step 1：Instrument is opened
Switch on power；This instrument uses battery as power supply, while opens light source（1）In 570nm visible light source
LED1 and 740nm visible light sources LED2；Instrument returns to zero, display module（8）For 0；
Step 2：Fresh tea leaf sample treatment
1）, tealeaves cleaning；
2）, weigh 1.0g fresh tea leafs and be positioned over heater（2）, add 10ml pure water, open heater（2）Agitator
1min, tealeaves is stirred evenly；
3）, toward heater（2）It is middle to add pH 8.0 the phosphate buffer 5ml, 2% ninhydrin 10ml configured；Open
Heating, set heat time 15min；
4）, open heater（2）With cooling device（3）Between valve（a）, by liquid level difference by heater（2）Flow to cold
But device（3）, open cooling device（3）In fan 2min, standing is cooled to normal temperature, closes cooling fan；
Step 3：The detection of testing sample
1）, tealeaves slurries stand cooling after, open cooling device（3）With reaction tank（4）Between air driven pump valve（b）, will
Supernatant liquor is drawn into reaction tank（4）In, system automatic start 570nm visible light source LED1 and 740nm visible light source
LED2；Tealeaves clear liquid is irradiated simultaneously, carries out UV absorption test；
2）, instrument display module（8）Show the testing result of theanine.
Table 1 is the Tea Samples of known theanine concentration, with the theanine concentration and error measured by portable instrument, phase
To error<15%, corresponding curve is as shown in Fig. 2 illustrate that this instrument can be used for quick detection theanine content.
Table 1
| Theanine concentration（mg/ml) | The theanine concentration (mg/ml) of Instrument measuring | Relative error % |
| 0 | 0 | 0.0 |
| 0.20 | 0.17 | 15.0 |
| 0.30 | 0.265 | 12.0 |
| 0.40 | 0.364 | 9.0 |
| 0.50 | 0.465 | 7.0 |
| 0.60 | 0.525 | 12.5 |
Step 4：The cleaning of instrument
1）, by heater（2）, cooling device（3）In container and reaction tank（4）Take out successively；
2）, respectively flushed three times with clear water, dry in case next time uses；
3）, finally by heater（2）, cooling device（3）In container and reaction tank（4）Reset into instrument.
Claims (2)
- A kind of 1. portable theanine detecting instrument, it is characterised in that：Including closed chamber and display module；Inside closed chamber successively For light source（1）, heater（2）, cooling device（3）, reaction tank（4）, model LSSPD-U4 Photoelectric Detection module（5）、 Model AD7715 modular converter（6）, model STC89C52RC control module（7）, display module（8）；The heating Device（2）, cooling device（3）, reaction tank（4）It is connected by pipeline, pipeline is provided with pneumatic operated valve（a）, air driven pump（b）；It is described Light source（1）Include producing 570nm visible light source LED1 and produce the LED2 of 740nm visible light sources.
- 2. with the method for the detecting instrument detection theanine described in claim 1, it is characterised in that：Comprise the following steps：Step 1：Instrument is openedSwitch on power, while open light source（1）In 570nm visible light source LED1 and 740nm visible light source LED2, instrument Zeroing；Step 2：Fresh tea leaf sample treatment1）Tealeaves cleans；2）Tealeaves is made by slurries by beater, takes filtrate in heater（2）It is interior；3）The phosphate buffers of pH 8.0 and 2% ninhydrin configured is added into filtrate, makes filtrate, the phosphoric acid of pH 8.0 Salt buffer, the volume ratio of 2% ninhydrin three are 1：1：0.5；4）Setting time heats；It is then turned on heater（2）With cooling device（3）In valve（a）, by liquid level difference by adding Thermal（2）Flow to cooling device（3）, open cooling device（3）In fan, standing be cooled to normal temperature；Step 3：The detection of testing sample1）After tealeaves slurries stand cooling, air driven pump, valve are opened（b）, supernatant liquor is extracted into reaction tank（4）In；Open simultaneously Open light source（1）In 570nm visible light source LED1 and 740nm visible light source LED2；Irradiate theanine sample, system simultaneously Automatically UV absorption test is carried out to tealeaves slurries；2）Instrument display module（8）Show theanine test result；Step 4：The cleaning of instrumentBy heater（2）, cooling device（3）In container and reaction tank（4）Take out successively；Clean, dry respectively, in case Next time uses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711106211.3A CN107796772A (en) | 2017-11-10 | 2017-11-10 | A kind of portable instrument and the method for detecting theanine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711106211.3A CN107796772A (en) | 2017-11-10 | 2017-11-10 | A kind of portable instrument and the method for detecting theanine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107796772A true CN107796772A (en) | 2018-03-13 |
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ID=61534778
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711106211.3A Pending CN107796772A (en) | 2017-11-10 | 2017-11-10 | A kind of portable instrument and the method for detecting theanine |
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| Country | Link |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102435565A (en) * | 2011-09-26 | 2012-05-02 | 黄山学院 | Quantitative measurement method for theanine |
| CN107202836A (en) * | 2017-04-11 | 2017-09-26 | 苏州市职业大学 | The rapid analysis method of theanine content in a kind of fresh sample of tealeaves |
-
2017
- 2017-11-10 CN CN201711106211.3A patent/CN107796772A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102435565A (en) * | 2011-09-26 | 2012-05-02 | 黄山学院 | Quantitative measurement method for theanine |
| CN107202836A (en) * | 2017-04-11 | 2017-09-26 | 苏州市职业大学 | The rapid analysis method of theanine content in a kind of fresh sample of tealeaves |
Non-Patent Citations (2)
| Title |
|---|
| 刘美 等: "茶叶中游离氨基酸含量的测定", 《微量元素与健康研究》 * |
| 韩立 等: "《仪器分析》", 31 October 2014, 吉林大学出版社 * |
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Application publication date: 20180313 |
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