CN107789377A - A kind of extract of actinidia arguta, preparation method and the usage - Google Patents
A kind of extract of actinidia arguta, preparation method and the usage Download PDFInfo
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- CN107789377A CN107789377A CN201610770727.7A CN201610770727A CN107789377A CN 107789377 A CN107789377 A CN 107789377A CN 201610770727 A CN201610770727 A CN 201610770727A CN 107789377 A CN107789377 A CN 107789377A
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- extract
- actinidia arguta
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- tara vine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
Abstract
The invention discloses a kind of extract of actinidia arguta, preparation method and the usage, belongs to autonomic drug field.A kind of preparation method of extract of actinidia arguta, step are：(1) dry：Tara vine is cleaned, normal temperature dries, and is dried under reduced pressure under the conditions of 30 50 DEG C 36 60 hours, obtains tara vine dry product；(2) extract：Tara vine dry product is broken into coarse powder, using carbon dioxide as solvent supercritical extract, solvent is reclaimed, obtains extract of actinidia arguta.The advantages of invention：Extract obtained by the present invention can retain the drug effect to relax bowel of tara vine to greatest extent, and low temperature drying and extraction ensure that the loss of the active ingredient of tara vine is preferably minimized.
Description
Technical field
The present invention relates to a kind of extract of actinidia arguta, preparation method and the usage, belongs to autonomic drug field.
Background technology
The wild date-plum persimmon in northeast claims monkey peach to be the valuable wild fruit resource of the mankind, and date-plum persimmon claims monkey peach (Actinl.diaargutasie
B.et Zucc) also known as bower actinidia fruit, claim monkey pears, rattan melon and rattan jujube, claim monkey peach category for Cheng Hou peaches section, perennial defoliation liana,
Northeast, North China, Shandong, northwest and the Yangtze river basin are distributed in, wherein THE SOUTH OF NORTHEAST CHINA mountain area is more common, its fruit succulence, and nutrition is rich
Richness, sour and sweet palatability, unique flavor " date-plum persimmon claims to be rich in Vc and edible cellulose in monkey peach, in fruit also containing volatile oil, carbohydrate,
The functional active components such as triterpene, flavonoids, vitamin, amino acid, mineral element, anti-oxidant, anti-aging, antifatigue and resist oxygen lack
Function in terms of function, radioresistance, raising immunity of organisms, anti-constipation, protection cardio-cerebrovascular.
For tara vine, current research mostly concentrates on food direction, then paid close attention to for its medical value it is less,
Especially its good functions of loosening bowel relieving constipation, the present invention provide a kind of preparation method of extract of actinidia arguta, and its preparation
Clinically treat the application of constipation.
The content of the invention
The invention solves first technical problem be to provide a kind of preparation method of extract of actinidia arguta.
The invention solves second technical problem be to provide a kind of extract of actinidia arguta.
The invention solves the 3rd technical problem be to provide extract of actinidia arguta be used for prepare treat constipation
The application of medicine.
To achieve the above object, the present invention uses following technical scheme.
A kind of preparation method of extract of actinidia arguta, step are：
1st, dry：Tara vine is cleaned, normal temperature is dried, and 36-60 hours are dried under reduced pressure under the conditions of 30-50 DEG C, are obtained
To tara vine dry product；
2nd, extract：Tara vine dry product is broken into coarse powder, with carbon dioxide (CO2) it is solvent supercritical extract, return
Solvent is received, obtains extract of actinidia arguta.
The vacuum of the step (1) is-0.08-- 0.1MPa, moisture≤15% of tara vine dry product.
Preferably, the temperature of the step (1) is 40 DEG C, is dried under reduced pressure 48 hours.
The step (2) is with CO2It is for solvent supercritical extract condition：Extracting pressure is 15-50MPa, and extraction temperature is
30-70 DEG C, supercritical CO2Flow is 10-45kg/h, and extraction time is 1-6 hours, and parsing pressure is 4-11MPa, resolution temperature
For 45-60 DEG C.
Preferably, the extraction conditions of step (2) is：Extracting pressure is 35MPa, and extraction temperature is 50 DEG C, supercritical CO2Stream
It is 4 hours to measure as 35kg/h, extraction time, and parsing pressure is 8MPa, and resolution temperature is 45 DEG C
In the step (2), during using carbon dioxide as solvent supercritical extract, one in addition methanol, ethanol and acetone
As entrainer, the mass ratio that entrainer is expected with extraction is 1 for kind or wherein several mixed solvents:1.5~20.
Further preferred following method：
1st, dry：Tara vine 1Kg is cleaned, normal temperature dries, and is put into and is dried under reduced pressure through net systems such as selections with a small amount of clear water
In case, it is dried under reduced pressure under the conditions of 40 DEG C about 48 hours, vacuum is-0.08-- 0.1MPa, obtains tara vine dry product；
2nd, extract：Tara vine dry product is broken into coarse powder, using carbon dioxide as solvent supercritical extract, added
100ml methanol is entrainer, extracting pressure 35MPa, and extraction temperature is 50 DEG C, and supercritical CO 2 flow is 35kg/h, during extraction
Between be 4 hours, parsing pressure be 8MPa, and resolution temperature is 45 DEG C, recovery solvent, drying, obtains extract of actinidia arguta.
Before the step (1) cleans tara vine, net system processing is carried out to Kiwi berry.
A kind of extract of actinidia arguta, is prepared using the above method.Confirm that the extract is certain through pharmacological evaluation
Softer stool can be played, the effect of promoting defecation.
The ripe tara vine of present invention selection, through net system, cleaning, after drying, is dried under reduced pressure under cryogenic conditions, obtains
To tara vine dry product, tara vine dry product is broken into coarse powder, carries out supercritical carbon dioxide extracting, extract warp
Mixed from appropriate auxiliary material so that different formulations are made, applied to clinic to prevent and treat constipation and relevant disease.
Extract of actinidia arguta of the present invention is preparing the application in treating and preventing constipation medicine, and it can be with pharmaceutically may be used
Carrier (auxiliary material) mixing of receiving, is made various formulations, the medicine of constipation is treated or prevented for preparing.
Extract of actinidia arguta of the present invention can assemble individually or with other medicines or other Chinese medical extracts,
The medicine of constipation is treated or prevented for preparing.
A kind of anti-medicine to treat constipation, the extract of actinidia arguta containing the present invention.
The medicine also contains pharmaceutically acceptable auxiliary material.
The medicine is peroral dosage form, including but not limited to tablet, capsule, powder, electuary etc..
The advantages of invention：Extract obtained by the present invention can retain relaxing bowel for tara vine to greatest extent
Drug effect, low temperature drying and extraction ensure that the loss of the active ingredient of tara vine is preferably minimized.
The present invention is elaborated with reference to embodiment, not limitation of the present invention.It is all to be disclosed according to the present invention
This area equivalent substitution that content is carried out, belongs to protection scope of the present invention.
Embodiment
Embodiment 1
1st, dry：Tara vine 1Kg is cleaned, normal temperature dries, and is put into and is dried under reduced pressure case through choosing net system with a small amount of clear water
In, it is dried under reduced pressure under the conditions of 40 DEG C about 48 hours, vacuum is -0.08MPa, obtains tara vine dry product；Date-plum persimmon macaque
The moisture of dried peach product is 14% (pharmacopeia oven drying method).
2nd, extract：Tara vine dry product is broken into coarse powder, using carbon dioxide as solvent supercritical extract, added
100ml methanol is entrainer, extracting pressure 35MPa, and extraction temperature is 50 DEG C, and supercritical CO 2 flow is 35kg/h, during extraction
Between be 4 hours, parsing pressure be 8MPa, and resolution temperature is 45 DEG C, recovery solvent, drying, obtains extract of actinidia arguta.
Embodiment 2
1st, dry：Tara vine 1Kg is cleaned, normal temperature dries, and is put into and is dried under reduced pressure through net systems such as selections with a small amount of clear water
In case, it is dried under reduced pressure under the conditions of 30 DEG C about 60 hours, vacuum is -0.09MPa, obtains tara vine dry product；Date-plum persimmon Mi
The moisture of monkey dried peach product is 10%.
2nd, extract：Tara vine dry product is broken into coarse powder, using carbon dioxide as solvent supercritical extract, adds 50ml
Ethanol is entrainer, extracting pressure 15MPa, and extraction temperature is 30 DEG C, and supercritical CO 2 flow is 10kg/h, extraction time 6
Hour, parsing pressure is 4MPa, and resolution temperature is 60 DEG C.Solvent is reclaimed, dries, obtains extract of actinidia arguta.
Embodiment 3
1st, dry：Tara vine 1Kg is cleaned, normal temperature dries, and is put into and is dried under reduced pressure through net systems such as selections with a small amount of clear water
In case, it is dried under reduced pressure under the conditions of 50 DEG C about 36 hours, vacuum is -0.09MPa, obtains tara vine dry product；Date-plum persimmon Mi
The moisture of monkey dried peach product is 12%.
2nd, extract：Tara vine dry product is broken into coarse powder, using carbon dioxide as solvent supercritical extract, added
600ml acetone is entrainer, and extracting pressure 50MPa, extraction temperature is 30 DEG C, supercritical CO2Flow is 45kg/h, during extraction
Between be 1 hour, parsing pressure is 11MPa, and resolution temperature is 45 DEG C.Solvent is reclaimed, dries, obtains extract of actinidia arguta.
Embodiment 4：It is prepared by oral tablet
First, prescription：
2nd, preparation method：
Extract of actinidia arguta 350g, starch 250g, sodium carboxymethyl starch 100g, microcrystalline cellulose are measured by prescription
50g, magnesium stearate 5g, mix, add the granulation of 70% appropriate amount of ethanol, dry, whole grain, tabletting, after passed examination, packaging.
Embodiment 5：Pharmacological evaluation
1st, experiment material
1.1 experimental animal
Wistar rats, male and female half and half, body weight 180-200g, Beijing Vital River Experimental Animals Technology Co., Ltd., close
Lattice card number：SCXK (capital) 2012-0001.
1.2 given the test agent
Extract of actinidia arguta (hereinafter referred to as extract, is prepared) by the method for embodiment 1, in Tianjin in new medicine company research
Heart Drug Manufacturing Room provide.
1.3 reagent
Carbon powder, gum arabic, Tianjin Kermel Chemical Reagent Co., Ltd..
2nd, experimental method
Wistar rats 30, male and female half and half, it is divided into 3 groups by body weight equilibrium：Blank control group, extract low dose group
(3g crude drugs/kg), extract high dose group (6g crude drugs/kg).Test the previous day each group Rat Fast more than 12 hours, gavage is given
Medicine (10mL/kg), blank control group give isometric pure water, while gavage 5% carbon powder gum arabic suspension 1ml/ only,
Rat is singly only put into metabolic cage, starts timing, observes first just time (row's melena time for the first time) of every animal and 8 small
When interior row's melena just grain number.Statistical comparison is carried out with blank control group, result of the test is counted using SPSS11.0.
3rd, experimental result
Influence of the extract of actinidia arguta of table 1 to normal rat defecating feces excretion
Compared with blank control group, * P<0.05, * * P<0.01.
Result of the test shows, two dosage group single-doses of extract, can substantially shorten normal rat head just times, simultaneously
Increase defecation grain number in 8 hours, more there is significant difference (P with blank control group<0.05, P<0.01).Prompt date-plum persimmon Mi
Monkey peach extract has obvious the effect of relaxing bowel.
Claims (10)
1. a kind of preparation method of extract of actinidia arguta, it is characterised in that step is：
(1) dry：Tara vine is cleaned, normal temperature is dried, and 36-60 hours are dried under reduced pressure under the conditions of 30-50 DEG C, is obtained soft
Jujube Kiwi berry dry product；
(2) extract：Tara vine dry product is broken into coarse powder, using carbon dioxide as solvent supercritical extract, solvent is reclaimed, obtains
To extract of actinidia arguta.
A kind of 2. preparation method of extract of actinidia arguta according to claim 1, it is characterised in that：The step
(1) vacuum is-0.08-- 0.1MPa, moisture≤15% of tara vine dry product.
A kind of 3. preparation method of extract of actinidia arguta according to claim 2, it is characterised in that：The step
(1) temperature is 40 DEG C, is dried under reduced pressure 48 hours.
A kind of 4. preparation method of extract of actinidia arguta according to claim 1, it is characterised in that：The step
(2) with CO2It is for solvent supercritical extract condition：Extracting pressure is 15-50MPa, and extraction temperature is 30-70 DEG C, supercritical CO2
Flow is 10-45kg/h, and extraction time is 1-6 hours, and parsing pressure is 4-11MPa, and resolution temperature is 45-60 DEG C.
A kind of 5. preparation method of extract of actinidia arguta according to claim 4, it is characterised in that：The step
(2) extraction conditions is：Extracting pressure is 35MPa, and extraction temperature is 50 DEG C, supercritical CO2Flow is 35kg/h, extraction time
For 4 hours, parsing pressure was 8MPa, and resolution temperature is 45 DEG C.
6. a kind of preparation method of extract of actinidia arguta according to any one of claim 1-4, its feature exist
In：In the step (2), during using carbon dioxide as solvent supercritical extract, addition methanol, ethanol and one kind in acetone or
For wherein several mixed solvents as entrainer, the mass ratio that entrainer is expected with extraction is 1:1.5~20.
7. the preparation method of a kind of extract of actinidia arguta according to claim 6, it is characterised in that step is：
(1) dry：Tara vine 1Kg is cleaned, normal temperature dries, and is put into and is dried under reduced pressure case through net systems such as selections with a small amount of clear water
In, it is dried under reduced pressure under the conditions of 40 DEG C about 48 hours, vacuum is-0.08-- 0.1MPa, obtains tara vine dry product；
(2) extract：Tara vine dry product is broken into coarse powder, using carbon dioxide as solvent supercritical extract, adds 100ml first
Alcohol is entrainer, and extracting pressure 35MPa, extraction temperature is 50 DEG C, and supercritical CO 2 flow is 35kg/h, and extraction time is 4 small
When, parsing pressure is 8MPa, and resolution temperature is 45 DEG C, reclaims solvent, dries, obtain extract of actinidia arguta.
8. a kind of extract of actinidia arguta, it is prepared using the method any one of claim 1-7.
9. a kind of anti-medicine to treat constipation, contains the extract of actinidia arguta described in claim 8.
10. the extract of actinidia arguta described in claim 8 is preparing the application in treating and preventing constipation medicine.
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| CN201610770727.7A CN107789377B (en) | 2016-08-30 | 2016-08-30 | Actinidia arguta extract, and preparation method and application thereof |
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| CN201610770727.7A CN107789377B (en) | 2016-08-30 | 2016-08-30 | Actinidia arguta extract, and preparation method and application thereof |
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| CN107789377B CN107789377B (en) | 2020-10-09 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111758812A (en) * | 2020-07-29 | 2020-10-13 | 赤壁神山兴农科技有限公司 | Kiwi fruit tea and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080207932A1 (en) * | 2005-04-29 | 2008-08-28 | Vital Food Processors Limited | Kiwifruit Oil Extraction Method And Product |
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2016
- 2016-08-30 CN CN201610770727.7A patent/CN107789377B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080207932A1 (en) * | 2005-04-29 | 2008-08-28 | Vital Food Processors Limited | Kiwifruit Oil Extraction Method And Product |
Non-Patent Citations (3)
| Title |
|---|
| 姜爱丽等: "软枣猕猴桃籽油的超临界萃取及成分分析", 《中国油脂》 * |
| 宫汝淳等: "软枣猕猴桃对家兔小肠平滑肌的影响", 《安徽农业科学》 * |
| 张晓丽等: "同干燥方法对猕猴桃总黄酮含量的影响", 《中国实验方剂学杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111758812A (en) * | 2020-07-29 | 2020-10-13 | 赤壁神山兴农科技有限公司 | Kiwi fruit tea and preparation method thereof |
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Address after: 300457 No. tenth, 21 Avenue, Tianjin economic and Technological Development Zone, Binhai New Area, Tianjin Applicant after: Research Institute Branch of Tianjin Zhongxin Pharmaceutical Group Co.,Ltd. Address before: 300457 No. tenth, 21 Avenue, Tianjin economic and Technological Development Zone, Binhai New Area, Tianjin Applicant before: R & D CENTER OF TIANJIN ZHONGXIN PHARMACEUTICALS |
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Effective date of registration: 20210512 Address after: 300457 R & D center, 21 10th Street, Tianjin Economic and Technological Development Zone, Binhai New Area, Tianjin Patentee after: Tianjin Zhongxin Pharmaceutical Research Institute Co.,Ltd. Address before: No.21, 10th Street, Tianjin Economic and Technological Development Zone, Binhai New Area, Tianjin, 300457 Patentee before: Research Institute Branch of Tianjin Zhongxin Pharmaceutical Group Co.,Ltd. |
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Effective date of registration: 20230411 Address after: 300457 R & D center, 21 10th Street, Tianjin Economic and Technological Development Zone, Binhai New Area, Tianjin Patentee after: Traditional Chinese Medicine Research Institute of Jinyao Darentang Group Co.,Ltd. Address before: 300457 R & D center, 21 10th Street, Tianjin Economic and Technological Development Zone, Binhai New Area, Tianjin Patentee before: Tianjin Zhongxin Pharmaceutical Research Institute Co.,Ltd. |