CN107789377B - Actinidia arguta extract, and preparation method and application thereof - Google Patents
Actinidia arguta extract, and preparation method and application thereof Download PDFInfo
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- CN107789377B CN107789377B CN201610770727.7A CN201610770727A CN107789377B CN 107789377 B CN107789377 B CN 107789377B CN 201610770727 A CN201610770727 A CN 201610770727A CN 107789377 B CN107789377 B CN 107789377B
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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Abstract
The invention discloses a actinidia arguta extract, a preparation method and application thereof, and belongs to the field of botanical drugs. A preparation method of actinidia arguta extract comprises the following steps: (1) and (3) drying: cleaning Actinidia arguta, air drying at normal temperature, and drying under reduced pressure at 30-50 deg.C for 36-60 hr to obtain dried Actinidia arguta; (2) extraction: crushing the dried actinidia arguta into coarse powder, performing supercritical extraction by using carbon dioxide as a solvent, and recovering the solvent to obtain the actinidia arguta extract. The invention has the advantages that: the extract obtained by the invention can maximally retain the effect of the actinidia arguta on relaxing bowels, and low-temperature drying and extraction ensure that the loss of effective components of the actinidia arguta is minimized.
Description
Technical Field
The invention relates to a actinidia arguta extract, a preparation method and application thereof, and belongs to the field of botanical drugs.
Background
The wild soft jujubes in northeast China are called as the kiwi fruits which are valuable wild fruit resources of human beings, the soft jujubes are called as the kiwi fruits (Actinl. biargotasieb. et Zucc), the soft jujubes are called as the soft jujubes, the monkey pears, the rattan melons and the actinidia chinensis, the actinidia family is called as the actinidia genus, the perennial deciduous vine plants are distributed in the watersheds of northeast, north China, Shandong, northwest and Yangtze river, wherein most mountainous areas in the northeast and south China are available, the fruits are juicy, rich in nutrition and palatable in sour and sweet, and the soft jujubes called as the actinidia chinensis are unique in flavor, and the soft jujubes are rich in Vc and edible cellulose, and the fruits also contain functional active ingredients such as volatile oil, saccharides, triterpenes, flavonoids, vitamins, amino acids, mineral elements and the like, and have the functions of resisting oxidation, aging, resisting fatigue.
For actinidia arguta, the current research is mostly focused on the food direction, the attention on the medicinal value of actinidia arguta is low, and particularly the good intestine moistening and bowel relaxing effects of actinidia arguta are achieved.
Disclosure of Invention
The invention aims to solve the first technical problem of providing a preparation method of actinidia arguta extract.
The second technical problem to be solved by the invention is to provide a actinidia arguta extract.
The third technical problem to be solved by the invention is to provide the application of the actinidia arguta extract in preparing the medicine for treating constipation.
In order to achieve the purpose, the invention adopts the following technical scheme.
A preparation method of actinidia arguta extract comprises the following steps:
1. and (3) drying: cleaning Actinidia arguta, air drying at normal temperature, and drying under reduced pressure at 30-50 deg.C for 36-60 hr to obtain dried Actinidia arguta;
2. extraction: crushing dried Actinidia arguta into coarse powder, adding carbon dioxide (CO)2) Extracting with solvent by supercritical extraction, and recovering solvent to obtain Actinidia arguta extract.
The vacuum degree in the step (1) is-0.08-0.1 MPa, and the water content of the dried actinidia arguta is less than or equal to 15%.
Preferably, the temperature of the step (1) is 40 ℃, and the drying is carried out for 48 hours under reduced pressure.
The step (2) is carried out with CO2The solvent supercritical extraction conditions are as follows: the extraction pressure is 15-50MPa, the extraction temperature is 30-70 ℃, and the supercritical CO is adopted2The flow is 10-45kg/h, the extraction time is 1-6 hours, the resolving pressure is 4-11MPa, and the resolving temperature is 45-60 ℃.
Preferably, the extraction conditions of step (2) are: the extraction pressure is 35MPa, the extraction temperature is 50 ℃, and the supercritical CO is adopted2The flow rate is 35kg/h, the extraction time is 4 hours, the analysis pressure is 8MPa, and the analysis temperature is 45 DEG C
In the step (2), when carbon dioxide is used as a solvent for supercritical extraction, one or more of methanol, ethanol and acetone is/are added as an entrainer, and the mass ratio of the entrainer to the extract is 1: 1.5-20.
The following methods are further preferred:
1. and (3) drying: cleaning 1Kg of actinidia arguta, selecting, cleaning with small amount of clear water, air drying at normal temperature, drying in a reduced pressure drying oven at 40 deg.C under reduced pressure for 48 hr with vacuum degree of-0.08-0.1 MPa to obtain dried actinidia arguta;
2. extraction: crushing the dried actinidia arguta into coarse powder, performing supercritical extraction by using carbon dioxide as a solvent, adding 100ml of methanol as an entrainer, performing extraction at the extraction pressure of 35MPa and the extraction temperature of 50 ℃, performing supercritical CO2 flow of 35kg/h for 4 hours, performing desorption at the desorption pressure of 8MPa and the desorption temperature of 45 ℃, recovering the solvent, and drying to obtain the actinidia arguta extract.
Before the actinidia arguta is cleaned in the step (1), the actinidia arguta is cleaned.
An Actinidia arguta extract is prepared by the above method. Pharmacological experiments prove that the extract can really play the roles of softening the excrement and promoting defecation.
The invention selects ripe actinidia arguta, after cleaning, cleaning and airing, the actinidia arguta is dried under reduced pressure at low temperature to obtain a dried actinidia arguta product, the dried actinidia arguta product is crushed into coarse powder, the coarse powder is subjected to supercritical carbon dioxide extraction, and the extract is mixed with proper auxiliary materials to prepare different formulations, so that the actinidia arguta can be clinically applied to prevent and treat constipation and related diseases.
The actinidia arguta extract can be mixed with a pharmaceutically acceptable carrier (auxiliary material) to prepare various dosage forms for preparing the medicine for treating or preventing constipation.
The actinidia arguta extract can be used alone or combined with other medicines or other traditional Chinese medicine extracts to prepare medicines for treating or preventing constipation.
A medicine for preventing and treating constipation contains the actinidia arguta extract.
The medicine also contains pharmaceutically acceptable auxiliary materials.
The medicament is an oral dosage form, including but not limited to tablets, capsules, powder, granules and the like.
The invention has the advantages that: the extract obtained by the invention can maximally retain the effect of the actinidia arguta on relaxing bowels, and low-temperature drying and extraction ensure that the loss of effective components of the actinidia arguta is minimized.
The present invention will be described in detail with reference to the following examples, which are not intended to limit the scope of the invention. Equivalents of the art made in accordance with the present disclosure are intended to be covered by the present invention.
Detailed Description
Example 1
1. And (3) drying: selecting and cleaning 1Kg of actinidia arguta, washing with a small amount of clear water, air drying at normal temperature, placing in a reduced pressure drying oven, drying under reduced pressure at 40 deg.C for 48 hr with vacuum degree of-0.08 MPa to obtain dried actinidia arguta; the water content of the dried actinidia arguta is 14% (pharmacopeia drying method).
2. Extraction: crushing the dried actinidia arguta into coarse powder, performing supercritical extraction by using carbon dioxide as a solvent, adding 100ml of methanol as an entrainer, performing extraction at the extraction pressure of 35MPa and the extraction temperature of 50 ℃, performing supercritical CO2 flow of 35kg/h for 4 hours, performing desorption at the desorption pressure of 8MPa and the desorption temperature of 45 ℃, recovering the solvent, and drying to obtain the actinidia arguta extract.
Example 2
1. And (3) drying: cleaning 1Kg of actinidia arguta, selecting, cleaning with small amount of clear water, air drying at normal temperature, placing into a reduced pressure drying oven, drying at 30 deg.C under reduced pressure for 60 hr with vacuum degree of-0.09 MPa to obtain dried actinidia arguta; the water content of the dried actinidia arguta is 10%.
2. Extraction: crushing the dried actinidia arguta into coarse powder, performing supercritical extraction by using carbon dioxide as a solvent, adding 50ml of ethanol as an entrainer, performing extraction at 15MPa and 30 ℃ under the supercritical CO2 flow rate of 10kg/h for 6 hours, and performing desorption at 60 ℃ and 4 MPa. Recovering solvent, and drying to obtain Actinidia arguta extract.
Example 3
1. And (3) drying: cleaning 1Kg of actinidia arguta, selecting, cleaning with small amount of clear water, air drying at normal temperature, placing into a reduced pressure drying oven, drying under reduced pressure at 50 deg.C for 36 hr with vacuum degree of-0.09 MPa to obtain dried actinidia arguta; the water content of the dried actinidia arguta is 12%.
2. Extraction: crushing dried Actinidia arguta into coarse powder, performing supercritical extraction with carbon dioxide as solvent, adding 600ml acetone as entrainer, extracting at 30 deg.C under 50MPa and supercritical CO2The flow rate was 45kg/h, the extraction time was 1 hour, and the resolving pressure was 11MPa, and the analysis temperature is 45 ℃. Recovering solvent, and drying to obtain Actinidia arguta extract.
Example 4: oral tablet preparation
Firstly, prescription:
secondly, the preparation method comprises the following steps:
weighing 350g of actinidia arguta extract, 250g of starch, 100g of sodium carboxymethyl starch, 50g of microcrystalline cellulose and 5g of magnesium stearate according to the formula, mixing, adding a proper amount of 70% ethanol, granulating, drying, granulating, tabletting, checking to be qualified, and packaging.
Example 5: pharmacological experiments
1. Experimental Material
1.1 Experimental animals
Wistar rats, male and female halves, weight 180-: SCXK (Jing) 2012 and 0001.
1.2 test samples
Actinidia arguta extract (hereinafter referred to as extract, prepared by the method of example 1) is provided by the preparation room of the research center of Tianjin Chinese New medicine industry.
1.3 reagents
Carbon dust, gum arabic, tamarix chemist limited, nji, tianjin.
2. Experimental methods
30 Wistar rats, half of which are divided into 3 groups according to weight balance: blank control group, extract low dose group (3g crude drug/kg), and extract high dose group (6g crude drug/kg). Rats in each group were fasted for more than 12 hours before the experiment, and were subjected to gastric gavage (10mL/kg), a blank control group was subjected to an equal volume of purified water, 1 mL/rat of a 5% carbon powder-gum arabic suspension was simultaneously subjected to gavage, a single rat was placed in a metabolic cage, timing was started, and the first stool time (first black stool time) and the number of black stool particles within 8 hours of each animal were observed. And (4) performing statistical comparison with a blank control group, and performing statistics on test results by using SPSS 11.0.
3. Results of the experiment
TABLE 1 Effect of Actinidia arguta extracts on the laxative Effect of Normal rats
P <0.05, P <0.01 compared to the blank control group.
Test results show that the single administration of two dose groups of the extract can obviously shorten the first defecation time of normal rats, increase the number of defecation grains within 8 hours, and have significant difference (P <0.05, P <0.01) compared with a blank control group. The suggestion is that the actinidia arguta extract has obvious effect of relaxing bowel.
Claims (8)
1. A preparation method of actinidia arguta extract for treating and preventing constipation is characterized by comprising the following steps:
(1) and (3) drying: cleaning Actinidia arguta, air drying at normal temperature, and drying under reduced pressure at 30-50 deg.C for 36-60 hr to obtain dried Actinidia arguta; the vacuum degree in the step (1) is-0.08-0.1 MPa, and the water content of the dried actinidia arguta is less than or equal to 15 percent;
(2) extraction: crushing the dried actinidia arguta into coarse powder, performing supercritical extraction by using carbon dioxide as a solvent, and recovering the solvent to obtain actinidia arguta extract; step (2) with CO2The solvent supercritical extraction conditions are as follows: the extraction pressure is 15-50MPa, the extraction temperature is 30-70 ℃, and the supercritical CO is adopted2The flow is 10-45kg/h, the extraction time is 1-6 hours, the resolving pressure is 4-11MPa, and the resolving temperature is 45-60 ℃.
2. The method for preparing actinidia arguta extract for treating and preventing constipation according to claim 1, wherein the method comprises the following steps: the temperature of the step (1) is 40 ℃, and the drying is carried out for 48 hours under reduced pressure.
3. Root of herbaceous plantThe method for preparing actinidia arguta extract for treating and preventing constipation according to claim 1, wherein the method comprises the following steps: the extraction conditions of the step (2) are as follows: the extraction pressure is 35MPa, the extraction temperature is 50 ℃, and the supercritical CO is adopted2The flow rate was 35kg/h, the extraction time was 4 hours, the desorption pressure was 8MPa, and the desorption temperature was 45 ℃.
4. The method for preparing actinidia arguta extract for treating and preventing constipation according to any one of claims 1 to 3, wherein: in the step (2), when carbon dioxide is used as a solvent for supercritical extraction, one or more of methanol, ethanol and acetone is/are added as an entrainer, and the mass ratio of the entrainer to the extract is 1: 1.5-20.
5. The method for preparing actinidia arguta extract for treating and preventing constipation according to claim 4, wherein the method comprises the following steps:
(1) and (3) drying: selecting 1Kg of actinidia arguta, cleaning with a small amount of clear water, air drying at normal temperature, placing in a reduced pressure drying oven, drying at 40 deg.C under reduced pressure for 48 hr with vacuum degree of-0.08-0.1 MPa to obtain dried actinidia arguta;
(2) extraction: crushing the dried actinidia arguta into coarse powder, performing supercritical extraction by using carbon dioxide as a solvent, adding 100ml of methanol as an entrainer, performing extraction at the extraction pressure of 35MPa and the extraction temperature of 50 ℃, performing supercritical CO2 flow of 35kg/h for 4 hours, performing desorption at the desorption pressure of 8MPa and the desorption temperature of 45 ℃, recovering the solvent, and drying to obtain the actinidia arguta extract.
6. An actinidia arguta extract for the treatment and prevention of constipation, prepared by the method of any one of claims 1 to 5.
7. A medicament for treating and preventing constipation, comprising the actinidia arguta extract of claim 6.
8. Use of the actinidia arguta extract of claim 6 for the preparation of a medicament for the treatment and prevention of constipation.
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Address after: 300457 No. tenth, 21 Avenue, Tianjin economic and Technological Development Zone, Binhai New Area, Tianjin Applicant after: Research Institute Branch of Tianjin Zhongxin Pharmaceutical Group Co.,Ltd. Address before: 300457 No. tenth, 21 Avenue, Tianjin economic and Technological Development Zone, Binhai New Area, Tianjin Applicant before: R & D CENTER OF TIANJIN ZHONGXIN PHARMACEUTICALS |
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Effective date of registration: 20230411 Address after: 300457 R & D center, 21 10th Street, Tianjin Economic and Technological Development Zone, Binhai New Area, Tianjin Patentee after: Traditional Chinese Medicine Research Institute of Jinyao Darentang Group Co.,Ltd. Address before: 300457 R & D center, 21 10th Street, Tianjin Economic and Technological Development Zone, Binhai New Area, Tianjin Patentee before: Tianjin Zhongxin Pharmaceutical Research Institute Co.,Ltd. |