CN1077765C - Rice wide-congenial restoring line and its heterozygotic combination breeding method - Google Patents

Rice wide-congenial restoring line and its heterozygotic combination breeding method Download PDF

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CN1077765C
CN1077765C CN98101799A CN98101799A CN1077765C CN 1077765 C CN1077765 C CN 1077765C CN 98101799 A CN98101799 A CN 98101799A CN 98101799 A CN98101799 A CN 98101799A CN 1077765 C CN1077765 C CN 1077765C
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rice
hybrid
wide
extensive
combination
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CN1203736A (en
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任光俊
陆贤军
张翅
李青茂
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CROP Research Institute of Sichuan Academy of Agricultural Sciences
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Abstract

The present invention relates to a recovery line of paddy rice and a breeding method of heterozygotic combination. Lemont is used as female parents, a Zhongxian recovery line 871028 is used as male parents to be hybridized with the female parents, and Chenghui 448 of the recovery line is bred. Zhenshan 97A, II-32A, Gang 46A, Fuyi A, Jin 23A, D62A, K17A or K18A is used as female parents, and the Chenghui 448 is used as male parents to breed hybrid. The Chenghui 448 has the characteristics of strong recovery force and stress resistance, wide affinity and adaptability, high yield of seed production, excellent quality, etc. The bred hybrid has the advantages of early mature and high yield. The average yield per mu of Shanyou 448 is 490.15 kilograms and is increased by 9.07% than that of Shanzhai No. 8, and the average yield per mu of II You 448 is 567.72 kilograms and is increased by 14.5% than that of Shanyou 195 by a region test.

Description

The selection of rice wide-congenial restoring line and assorted excellent combination thereof
Technical field:
The invention belongs to the rice heterosis utilization technical field, be specifically related to a kind of seed selection of paddy rice new restorer line and the high-yield seeds production technique of crossbreed thereof.
Background technology:
Paddy rice is one of staple food crop of China, is national people's grain ration.The development Rice Production is maintained social stability for ensureing food security, and boosting economic growth all has crucial meaning.
Hybrid rice is the principal item type on China's Rice Production, cultivates more high yield of high yield, and the new combination that adapts to different cropping systems is the vital task of breeding man.Utilizing indica-japonica heterosis is the technological approaches of seed selection super high-yielding hybrid rice, but hybrid F 1And the offspring super close late-maturing, plant is higher, technological difficulties such as hollow kernels have influenced the progress of this approach.Country's 86.3 plans utilize the emphasis of tackling key problems as rice breeding to the two line method indica-japonica heterosis, and the stable three line method approach of sterility does not cause enough attention.
The slow ripe combination of Xian in Shanyou 63, the ridge excellent 22 etc. is promoted in hybridization such as Sichuan medium rice head of district's phase, though make huge contribution to increasing production paddy, because of breeding time slow partially, late fall, the habitat failed to make full use of, the rice field cropping index is not high, overall economic efficiency is relatively poor.Disease resistance descends simultaneously, and rice matter is medium, haves much room for improvement.The rice district dry season that has is serious, cuts off the water supply early in the field, and slow ripe combination ripening rate is low, the not high shakiness of per unit area yield.In semilate rice+ratoon rice district, semilate rice is generally slow ripe combination, first rice crop high yield, but often To yield of ratooning crop fluctuation is bigger, do not become real one season the crops.Therefore, select for use suitable genetic germplasm to do the parent, set up breeding technique system efficiently, the disease-resistant new restorer line of wide affine high-quality of ripe type in the cultivation, by the nucleo-cytoplasmic interaction male sterility with different maturation period is extensive test cross, can assembly go out the high-quality of various ripe phases, disease-resistant, high-yield hybrid rice.
Purpose of the present invention is intended to solve the problem in aforementioned breeding technique and the Rice Production, provide a kind of new restorer line of excellence and preparation precocious crossbreed, in ripe crossbreed and supporting high-yield seeds production technique, produce the hybrid rice seed of amount foot of fine quality, can one season the medium rice district, the demonstration of semilate rice+ratoon rice district, southern double rice cropping system with apply.
The invention scheme:
The selection of a kind of wide affine rice restorer and assorted excellent combination thereof.Selection of the present invention is realized by following steps:
(1) be maternal with Lemont, it is 871028 to be paternal hybrid, hybrid F that middle Xian recovers 2It is choosing that generation begins, F 3-F 5In generation, carried out restoring force and combining ability test, and select blast resisting plant, F 6In generation, carried out anther culture, seed selection wide-congenial restoring line (becoming extensive 448);
(2) be maternal with Zhenshan 97a, II-32A, Gang No.46A, Fuyi No.A, golden 23A, D62A, K17A or K18A, with wide-congenial restoring line (becoming extensive 448) is male parent, preparing hybrid kind (Shan is excellent 448, II is excellent 448, the ridge is excellent 448, good fortune is excellent 448, Jin You 448, D are excellent 448, K excellent 448 or K excellent 448).
Beneficial effect of the present invention is as follows:
1, the beneficial effect of one-tenth extensive 448
(1) restoring force is strong, and general combining ability is good: a plurality of male sterile line test crosses such as this recovery system and Zhenshan 97a, Gang No.46A, II-32A, D62A, K17A, big grain A, F 1The ripening rate in generation is 80.22-90.06%, average out to 83.3%.F with these male sterile line combos 1The existing stronger output hybrid vigour of representative.Precocious combination such as Zhenshan 97a/448 grades on average increases production 12.37% than with the excellent 64 volume increase 8.03-16.06% of the Shan of ripe phase.In ripe combination II-32A/448, D62A/448 compare respectively according to the volume increase 18.22%, 12.02%.From analysis of Yield Forming Factors, the number of grain per ear advantage of hybrid is remarkable, and thousand kernel weight is higher, and heavy fringe is the main cause of volume increase.
(2) have wide compatibility: become extensive 448 with long-grained nonglutinous rice test kind of a Nanjing 11, IR36 hybridization, F 1In generation,, average ripening rate was 85.89%; With japonica rice test kind of a Bali draw, the autumn flash ranging hands over F 1In generation,, average ripening rate was 69.04%.This illustrates that this recovery system not only can be with long-grained nonglutinous rice male sterile line combo, also can be with the male sterile line test cross of osculant or japonica-cline type, the hybrid rice that the preparation advantage is stronger.
(3) strong stress resistance.Through Sichuan, the Institute of Plant Protection of Zhejiang, Hunan, Guangdong Si Sheng academy of agricultural sciences identifies leaf pest 0-4 level, neck pest 1-3 level.6.1 grades of bacterial leaf-blight average out to, 5.6 grades of warm tolerances.
(4) rice quality is excellent.Measure according to cereal chemistry system of rice in China institute: become extensive 448 brown rice rates 77.1%, polished rice rate 70.7%, head rice rate 64.7%, grain of rice aspect ratio is 3.0, the white grain of chalk rate 9%, chalkiness degree 0.7%.It is 7.0 that 3 grades of transparencies, alkali are cleared up value, glue denseness 76mm, amylose content 12.8%.Have 5 to reach one-level rice standard, 4 reach the second heads rice standard.
(5) ecologic adaptation is wide.It is medium to the reaction of light temperature that this recovers system.Long-grained nonglutinous rice district, south all can be used for the production of hybrid seeds.
(6) with long-grained nonglutinous rice male sterile line combo, hybrid F 1Breeding time incline early.Help cultivating the precocious combination of super high-yielding and in ripe combination.
(7) hybrid seed yield height: this recovery is a middle seasonal strain, and is shorter with the sowing difference phase of male sterile line, and the florescence is easy to regulate.Become extensive 448 property in great numbers good, flower pesticide is bigger, and the loose powder performance is good, favourable raising outcrossing rate.With the Zhenshan 97a combo, per unit area yield reaches more than 200 kilograms; Break through 300 kilograms with II32-A production of hybrid seeds per unit area yield.
2, become the beneficial effect of the crossbreed of extensive 448 preparations
(1) the precocious combination of Xian Shan excellent 448 in the super high-yielding
1996-1997 participates in the precocious group of Xian regional test in the Sichuan Province, and average yield per mu 490.15kg comparison is according to narrow No. 8 volume increase 9.07% of Shan.Sub-district folding per mu yield 557.7kg was than excellent 64 volume increase 12.05% of Shan in the variety comparative test of Chengdu in 1996.Outward appearance, food flavor quality are better than contrast, blast resisting, and plant and leaf shape is excellent, and stalk is slightly anti-, and integral economic trait is good.
This combination 1996 is planted experimentally 513 kilograms of first rice crop per mu yields, ratoon rice per mu yield 560kg, 1073 kilograms of two season per mu yields for 1100 meters at Yunnan Shuan Jiangxian height above sea level.
(2) ripe combination II excellent 448 among the Xian in the super high-yielding
1996-1997 participates in ripe group of district's examination among the Xian in the Sichuan Province, 567.72 kilograms of average yield per mus, and comparison is according to excellent 195 volume increase 14.51% of Shan, and outward appearance and food flavor product are of fine quality, and disease resistance is strong, and warm tolerance is better.
This combination regeneration ability is strong, and in the Shuan Jiangxian paddy rice introduction and Experiment of Yunnan, 1086 kilograms in semilate rice+ratoon rice mu occupies first.
Embodiment:
One, recover to be tied to form extensive 448 and the concrete seed selection process of crossbreed be:
1, with the high-quality that originates in the U.S., extensively in affine, naked body seed rice Lemont and the China Xian to recover be 871028 hybridization, seed selection has the wide affine new restorer line of indica of naked body rice heredity blood relationship, to improve the hybrid vigour level, improves rice quality.F 2It is choosing that generation begins, F 3-F 5In generation, carried out restoring force and combining ability test, and blast resisting is identified F 6In generation, carried out anther culture, carries out compatibility, strong advantage combined sorting subsequently.Adopt these comprehensive breeding technology, breed that output coordinate force height, blast resisting, good grain quality, affinity are strong, the new restorer line of wide adaptability becomes extensive 448.
2, being male parent with recovering to be tied to form extensive 448, is female parent with male sterile line Zhenshan 97a, the II-32A of large tracts of land production usefulness, breed high yield, high-quality, disease-resistant in ripe crossbreed II excellent 448 and precocious crossbreed Shan excellent 448.
3, good recovery is tied to form extensive 448 for male parent, also with other male sterile line, as Gang No.46A, Fuyi No.A, golden 23A, D62A, combo excess of export high-yield early-maturing hybrid rices such as K17A, K18A.
Two, wide-congenial restoring line become extensive 448 and the seeding technique of crossbreed be:
With the wide affine naked body rice of the U.S. is important basic germplasm, takes conventional breeding method and modern biotechnology, breed have long-grained nonglutinous rice and naked body rice heredity blood relationship, wide affine new restorer line that general combining ability is good becomes extensive 448.When the production of hybrid seeds, because of ripe type in being its breeding time, the florescence is comparatively concentrated, generally speaking, adopts the male parent production of hybrid seeds of two phases to be advisable.
Rice wide-congenial restoring line become extensive 448 and assorted excellent combination seed selection process see attached list one.
Three, parental source and feature thereof
1, parental source: 1989, these used 870128 indica restorer lines such as grade and Lemont hybridization were adopted pedigree method, the wide affine new restorer line of continuous multi-generation test cross seed selection.871028 polymerizations common wild-rice, IR24, many anti-, high-qualitys such as bright extensive 63, high-combining ability and eurytopicity relationship.Lemont is the famous kind in U.S. paddy rice producing region, is one of famous in the world high light efficiency germplasm.From F 2In generation, rise, and the selected plant blade profile is excellent emphatically, and lodging resistant force is good; In slow ripe type, solid good individual plant or the strain system of enriching gives birth to nuclear with the court and cooperates, to the F of bigger separation is still arranged 6Strain system adopts anther culture method, the good type of isozygotying, the survey of male sterile lines such as middle advanced lines material and Zhenshan 97a, II-32A is joined, and draw with Bali, the wide affine test kind test cross of round-grained rice type such as Qiu Guang; Cooperate with the Institute of Plant Protection, the court.Identify the anti-pest of filial generation in rice blast Chang Faqu artificial infection of Pujiang County, Sichuan and spontaneous induction.Integrated use these conventional breeding method and modern biotechnologies are cultivated successfully wide affine new restorer line and are become extensive 448.
2, feature: tillering ability is stronger, it is suitable that plant type is tightly loose, leaf look pale green, and narrower, the thick and straight elevator of blade, anti-fertilizer, lodging resistant force are strong, the fringe neck is short, the fringe type long, grain close, that grain is many, thousand kernel weight is high, the grain husk point is colourless, 95 centimetres of plant heights, 132 of every fringe grains per panicles, ripening rate 91%, thousand kernel weight 29.4 grams, the outward appearance of rice, processing and food flavor quality are all more excellent.
Become extensive 448 to have wide compatibility, with long-grained nonglutinous rice test kind of hybridization, F 1Ripening rate is 85.89%; With japonica rice test kind of hybridization, F 1Ripening rate 69.04%.This blast resisting ability of recovering system is strong, according to " 95 " national rice breeding tackling key problem " CHARACTERISTICS IDENTIFICATION and evaluation " cooperative groups in Zhejiang, the associating qualification result in Hunan, Guangdong, 2.0 grades of leaf pest, 2.6 grades of panicle blasts.
Sow in spring in the area, Chengdu, sowing to the phase of going through of the fringe that begins is 102 days, lacks 7 days than bright extensive 63.The average number of blade 15.5 leaves of stem.The pollen amount foot, pollen density is big, and loose powder is good, and pollen is energetic, and florescence 10-12 days, more concentrated during flower, fine day 11:30-12:30 in the morning spent the period for containing.
Four, become the crucial seeding technique of the new combination of extensive 448 preparing hybrid rice
1, becomes extensive 448 broadcast and take out the phase of going through than bright extensive 63 short 7 days.Average blade 15.5 leaves of stem lack the 1-1.5 leaf than bright extensive 63.Thereby any one male sterile line is when becoming extensive 448 production of hybrid seeds, regulates the principle of the time difference and should lack 6-7 days than with bright extensive 63 production of hybrid seeds time.
2, one-tenth extensive 448 with the accent phase of the II-32A production of hybrid seeds is: the 1st phase of male parent and П-32A sows the same period, and the male parent second phase was than the 1st phase late sowing 5-6 days.
3, one-tenth extensive 448 with the concrete accent difference of the Zhenshan 97a production of hybrid seeds is: about 44 days time differences, leaf age differs from about 8.6 leaves.
4, one-tenth extensive 448 with the concrete accent difference of the Gang No.46A production of hybrid seeds is: about 41 days time differences, leaf age differs from about 8.2 leaves.
5, one-tenth extensive 448 with the concrete accent difference of the Fuyi No.A production of hybrid seeds is: about 40 days time differences, leaf age differs from about 8.0 leaves.
6, one-tenth extensive 448 with the concrete accent difference of the golden 23A production of hybrid seeds is: about 49 days time differences, leaf age differs from about 9.6 leaves.
7, one-tenth extensive 448 with the concrete accent difference of the D62A production of hybrid seeds is: about 34 days time differences, leaf age differs from about 6.5 leaves.
8, one-tenth extensive 448 with the concrete accent difference of the K17A or the K18A production of hybrid seeds is: about 45 days time differences, leaf age differs from about 8.6 leaves.
Subordinate list one:
The wide affine new restorer line of paddy rice becomes extensive 448 assorted excellent combination seed selection process place generation technical measures in season in table time 1989 summer Chengdu 871028/Lemont preparation combination 1989 winter Hainan F 1Mix and receive 1990 summer Chengdu F 2Choose osculant of short stem or 1990 winter of indica individual plant Hainan F 3Select good 1991 summer of the excellent strain Chengdu F of fecundity 4Choose ripe individual plant of short stem, blast resisting is identified 1992 summer Chengdu F 5Choose the good individual plant of ripe fecundity of short stem, 1993 summer of blast resisting dientification of bacteria Chengdu F 6Select the elite plant strain anther culture, blast resisting is identified 1993 winter Hainan H 1Choose ripe fine individual plant and Zhenshan 97a, II-32A and japonica rice test kind of paired test cross.1994 summer Chengdu H 2Combination advantage is observed.20844 output coordinate force height, compatibility is arranged, the agronomy neat and consistent, strong in lesion performance disease resistance, name to becoming extensive 448.1994 winter Hainan H 3With Zhenshan 97a, the II-32A production of hybrid seeds.1995 summer Chengdu H 4Make up production ratio, anti-pest evaluation, parent propagation, combined copy.1996-1997 Sichuan participate in the precocious group of Xian in the Sichuan Province, in ripe group of district's examination and production test, formally name new combination Shan excellent 448 and II excellent 448.Become extensive 448 wide compatibilities to identify again.Yunnan Shuan Jiangxian ratoon rice test.

Claims (1)

1, the selection of a kind of wide affine rice restorer and assorted excellent combination thereof, it is characterized in that: the present invention is realized by following steps:
(1) be maternal with Lemont, it is 871028 to be paternal hybrid, hybrid F that middle Xian recovers 2It is choosing that generation begins, F 3-F 5In generation, carried out restoring force and combining ability test, and select blast resisting plant, F 6In generation, carried out anther culture, seed selection wide-congenial restoring line (becoming extensive 448);
(2) be maternal with Zhenshan 97a, II-32A, Gang No.46A, Fuyi No.A, golden 23A, D62A, K17A or K18A, with wide-congenial restoring line (becoming extensive 448) is male parent, preparing hybrid kind (Shan is excellent 448, II is excellent 448, the ridge is excellent 448, good fortune is excellent 448, Jin You 448, D are excellent 448, K excellent 448 or K excellent 448).
CN98101799A 1998-05-12 1998-05-12 Rice wide-congenial restoring line and its heterozygotic combination breeding method Expired - Fee Related CN1077765C (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1107286A (en) * 1994-03-18 1995-08-30 中国水稻研究所 Method for breeding of cross-breeding rice
CN1131505A (en) * 1995-06-14 1996-09-25 江苏沿海地区农业科学研究所 Cultural method for optimum restoring line

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1107286A (en) * 1994-03-18 1995-08-30 中国水稻研究所 Method for breeding of cross-breeding rice
CN1131505A (en) * 1995-06-14 1996-09-25 江苏沿海地区农业科学研究所 Cultural method for optimum restoring line

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