CN1037483C - Method for breeding of cross-breeding rice - Google Patents
Method for breeding of cross-breeding rice Download PDFInfo
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- CN1037483C CN1037483C CN 94102551 CN94102551A CN1037483C CN 1037483 C CN1037483 C CN 1037483C CN 94102551 CN94102551 CN 94102551 CN 94102551 A CN94102551 A CN 94102551A CN 1037483 C CN1037483 C CN 1037483C
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Abstract
The present invention relates to a breeding method of hybrid paddy rice, which belongs to the field of paddy rice heterosis utilization. The rotational 422 is used as male parents, and the rotational 10 to 19 is selected to be as female parents to be hybridized with the male parents. The wide affinity recovery line 50465 is bred to obtain 413 with the advantage expression and the field resistance condition of the hybrid F1 as selection indexes by separation and selection for multiple times. Xieqingzao A, Zhenshan 97A or 20A and Maxie A are used as female parents, the Zhong 413 is used as male parents to respectively bred hybridized combinations, Youxie 413, Shanyou 413, Weiyou 413 and Maxieyou 413. The present invention has the advantages that the Zhong 413 belongs to a wide affinity recovery line and has obvious heterosis of large ears, multiple grains, strong tillering power, enough pollen amount, easy seed production, large amplitude of the increase in yield and good resistance.
Description
The present invention relates to the rice heterosis utilization field.
After the seventies, hybrid rice is in China's seed selection success and be applied to production, generally increases production more than 20% than conventional variety.But, all genus intervarietal cross rice of using in the production, the advantage limitation is bigger.The intervarietal cross combination amount of increase in production of breeding in recent years is less.The inter-subspecies hybrid paddy rice has the hybrid vigour huger than intervarietal cross paddy rice.Comparable in theory existing hybrid combination volume increase is more than 20%, but it is on the low side also to exist the hybrid ripening rate, hollow kernels, and the ripe phase, slow partially and plant crossed high shortcoming.Directly utilize inter-subspecific heterosis still at the experimental stage.
It is on the low side to the objective of the invention is to solve the hybrid ripening rate that present inter-subspecies hybrid paddy rice exists, and the ripe phase is slow partially, shortcomings such as the higher and hollow kernels of plant, in the seed selection wide-congenial restoring line 413 and the new combination of subspecies super high-yielding in 413 serial breeding methods.
Content of the present invention:
1, seed selection is wide blue or green and recover in the system 413:
1989 introduce a fine variety by hybrid rice center, Hunan from Wuhan University is the Java type wide-congenial restoring line 50465 that selects the 10-19 selection cross with wide affine kind samsara 422 and strong the recovery, in the Nan Fan base plantation of Lingshui, Hainan Province county rice in China institute, find that 50465 exist bigger separation.Promptly carry out the individual plant biparental cross, receive in pairs and plant with the short type cytoplasmic sterile line Xieqingzao A that loses.Nineteen ninety sows in spring in Hangzhou rice in China institute test site., obtains a grain type and shorten as selecting index with the performance of the advantage of hybrid F1 and field resistance situation, the strain that the ripe phase is done sth. in advance and the Xieqingzao A combo has a clear superiority in is, promptly in 413.It is to be male parent with samsara 422, is hybridization of female parent to select 10-19, seed selection wide-congenial restoring line 50465, with the advantage performance of hybrid F1 and field resistance situation as selecting index, through separating for several times, superseded, in the acquisition 413.
In 413 feature: in 413 in Hangzhou sowing on May 8, beginning August 8 fringe, sowing is to beginning about 90 days fringe phases the 132 days time of infertility.Seedling quality is good, and growth potential is strong, has early to give birth to fast long characteristics.About the plant height 110cm, cane is sturdy, well developed root system, and lodging resistance is strong, and plant type is moderate, and sword-like leave is tall and straight upright, and the leaf look dark green, and photosynthesis is stronger.The stem number of blade 17.8 leaves.Tillering ability is strong, and seed farm individual plant tiller number reaches 47.3, and the individual plant number of productive ear is 34.7, several 200~250 of general total grain panicle, the seed shape becomes wealthy ovum type by 50465 slender type, and ripening rate is more than 80%, because big panicle many grains per panicle has a little back-grouting phenomenon.About thousand kernel weight 27g, about the general per mu yield 450Kg.More than seed farm per mu yield 150~200Kg.Rice matter is medium, brown rice rate 82.02%, polished rice rate 74.57%, the white rate 9.5% of chalk, 3 grades of transparencies, gelatinization point 6.7, glue denseness 36mm, amylose content 26.6%, protein content 11.26%. resistance is better, and middle bacterial blight-resisting is 3.9 grades to the average sick level of the resistance of 7 bacterial leaf-blight pathogenic bacteria, resistance result to rice blast is: anti-A group 17,53, in biological strain such as anti-B group 1, c group 1, D group 5, E group 1, biological strains such as middle sense A group 1, B group 17, sense A group 39, B group 5, C group 5, D group 1 biological strain such as grade.Brown planthopper also there is certain resistance.
2, the seed selection of hybrid combination
(1), be maternal with the Xieqingzao A, in 413 make up Xieyou 413 for the male parent preparing hybrid.
The principal character characteristic:
Xieyou 413 is suitable breeding time, and no Xian round-grained rice is handed over the obviously slow partially phenomenon of combination.Do single plantation in evening, the time of infertility, average out to was 142 days, and comparison is done two plantations in evenings according to short 3 days of kind Shanyou 63 (145), and the 132 days time of infertility was than long 2 days of Shan excellent No. 10 (130).Plant height 95~110cm, than about the short 10cm of Shanyou 63, cane is sturdy upright, the plant type compactness; The leaf look dark green, mesophyll is thicker, and the leaf appearance is tall and straight; Well developed root system, fertilizer resistance anti-lodging.Tillering ability is stronger, and the seedling number of productive ear can reach 18~200,000 fringes.Big panicle many grains per panicle, several 134.2~172.7 of every total grain panicle, several 125~153.9 of the real grain of every fringe, ripening rate 75.2~93.17%.Growth potential is prosperous, and later stage functional leaf decline is slow, not early ageing, and ripe lover, filling speed is fast, and the seed circularity is better, thousand kernel weight 26~29 grams.Do single plantation in evening, average yield per mu 583.65Kg is than Shanyou 63 volume increase 13.97%; Do two plantations in evening, average yield per mu 539.79Kg is than excellent No. 10 volume increase 13.40% of Shan.High anti-seasonal febrile diseases, middle bacterial blight-resisting, anti-brown back rice plant hopper.Rice matter is on the upper side medium.Brown rice rate 83.4%, polished rice rate 75.7%, head rice rate 67.3%, husked rice matter is better than Shanyou 63, the long 6mm of seed, wide 2.5mm, aspect ratio 2.4, the white rate 97% of chalk, chalkiness degree 38.32%, 4 grades of transparencies, gelatinization point 6.4, glue denseness 56mm, amylose content 24.2%, protein content 12.73% is higher by 3.9% than Shanyou 63, comprehensive, belong to same type with quality award from the ministry secondary superior rice Shanyou 63.
(2) be maternal with the Zhenshan 97a, in 413 make up Shanyou 413 for the male parent preparing hybrid
The principal character characteristic:
Single late plantation of Shanyou 413 work, be 140 days the time of infertility, and comparison was shone Shanyou 63 (145 days) short 5 days, and work is even cultivated evening, and the time of infertility, average out to was 133 days, and comparison was shone excellent No. 10 (130 days) length of Shan 3 days.Plant height 92~116cm and Xieyou 413 are close, and plant type is moderate, and cane is sturdy, lodging resistance is strong.The leaf look dark green, and sword-like leave is tall and straight, and photosynthesis is stronger.Well developed root system, tillering ability is stronger, and Gao Miaoke reaches about 230,000, and general mu is fringe 18~200,000 fringes effectively.About spike length 22cm, suitable with Shanyou 63, but big panicle many grains per panicle, granule density apparently higher than Shanyou 63, several 144.8~198.5 of every total grain panicle, several 128.7~171.7 of the real grain of every fringe, ripening rate 71.6~91.83%, seedling stage, seedling quality was good, and growth potential is prosperous, later stage functional leaf decline slowly, annesl is good, not early ageing, and kernel grouting speed is slower, circularity is good, and general thousand kernel weight can reach 26~29.3 grams.Do single plantation in evening, average yield per mu 545.84Kg than Shanyou 63 volume increase 9.42%, makes to plant two evenings average yield per mu 525.76Kg, than excellent 10 volume increase 15.59% of Shan.The Shanyou 413 disease and insect resistance is poorer slightly than Xieyou 413, but is better than Shanyou 63.In anti-seedling rice blast and panicle blast, gently feel bacterial leaf-blight.Rice matter is on the upper side medium, brown rice rate 83.1%, polished rice rate 75.3%, seed length 5.3mm, wide 2.6mm, aspect ratio 2.4, the white rate 79% of chalk, 3 grades of transparencies, gelatinization point 5.2, glue denseness 49mm, amylose content 19.4%, protein content 12.63%.
(3), be maternal with the Wei 20A, in 413 make up Weiyou 413 for the male parent preparing hybrid
The principal character characteristic:
Weiyou 413 is done single cropping rice plantation, in the 127 days time of infertility, lacks 4 days than Shanyou 63.Do two plantations in evening, the 131 days time of infertility is close with Shan excellent 10.About plant height 95cm, more than the short 15cm of Shanyou 63.Cane is more more sturdy than Xieyou 413, well developed root system, fertilizer resistance anti-lodging.The plant type compactness, the leaf look dark green, and sword-like leave is tall and straight, stem total leaf number about 16, seedling quality is good, and growth potential is prosperous, and tillering ability is stronger, and generally Gao Miaoke reaches 230,000, and mu is fringe about 200,000 effectively, and the percentage of earbearing tiller is apparently higher than excellent No. 10 of contrast Shan.But the comparison of total grain panicle number reaches 145.6 according to many more than 30%, ripening rate about 80%, and filling speed is fast, not early ageing of later stage, annesl is better, and thousand kernel weight can reach more than 27 grams.Make single plantation average yield per mu in evening 556.88Kg, than Shanyou 63 volume increase 5.13%, make to plant two evenings average yield per mu 544.93Kg, than excellent No. 10 volume increase 9.10% of Shan, the Weiyou 413 disease and insect resistance is better than Shanyou 413, but slightly is worse than Xieyou 413.Blast resisting is gently felt bacterial leaf-blight.Rice matter is medium, brown rice rate 83.5%, polished rice rate 75.0%, head rice rate 64.3%, the long 6.0mm of seed, wide by 2.7, aspect ratio 2.2, the white rate 98% of chalk, 3 grades of transparencies, gelatinization point 6.5, glue denseness 51mm, amylose content 24.1%, protein content 11.75%.
(4), be maternal with the Maxie A, in 413 make up Maxieyou 413 for the male parent preparing hybrid
The principal character characteristic:
Maxieyou 413 is done single cropping rice plantation, and is the 141 days time of infertility, identical with Shanyou 63.Do the double cropping late rice plantation, in the 130 days time of infertility, grow 1 day than Shan excellent 10.About plant height 96cm, about Shan excellent 10 high 0.5cm.But it is obviously short in Shanyou 63.Cane is sturdy, well developed root system, fertilizer resistance anti-lodging.Plant type is compact, and the leaf look dark green, and sword-like leave is tall and straight.Rice shoot is sturdy, early growth and quick tiller, and tillering ability is stronger.Every mu 250,000 of the highest seedling, the percentage of earbearing tiller is more than 75%, and general mu is fringe about 190,000 effectively, big panicle many grains per panicle, several 133.7 of every total grain panicle, excellent No. 10 (109.5) Duo 22.1% than Shan, several 107.6 of the real grain of every fringe, ripening rate about 80.44%, growth potential is prosperous, later stage functional leaf decline is slow, and annesl is good, not early ageing.The seed circularity is better, and thousand kernel weight is more than 26 grams, and economic coefficient reaches 0.58 5.Make single plantation average yield per mu in evening 540.6Kg, than Shanyou 63 volume increase 8.9%, make to plant two evenings average yield per mu 532.5Kg, than excellent No. 10 volume increase 5.68% of Shan.Better resistance, high blast resisting, middle bacterial blight-resisting.Rice matter is on the upper side medium, brown rice rate 83.5%, polished rice rate 75.9%, head rice rate 69.4%, the long 6.0mm of seed, wide 2.5mm, aspect ratio 2.4,, the white rate 90% of chalk, the white size 36% of chalk, 3 grades of transparencies, gelatinization point 6.3, glue denseness 68mm, amylose content 25.8%, protein content 11.72%.
Advantage of the present invention and good effect:
In 413 belong to wide-congenial restoring lines, lose with the open country, the short dissi of losing and horse hair account for and lose male sterile line hybridization, has tangible hybrid vigour, as Xieqingzao A and in the super high-yielding combination Xieyou 413 that forms of 413 combos, 1994 in Zhejiang Province 10 counties and cities' large-scale demonstrations, single cropping per mu yield 573.0~694.7Kg, comparison is according to Shanyou 63 volume increase 4.83~38.17%, connect late per mu yield 493.3~585.48Kg, comparison is according to excellent 10 volume increase 5.75~40.50% of Shan.And better resistance, high blast resisting, middle bacterial blight-resisting, anti-brown back rice plant hopper.In 413 big panicle many grains per panicles, tillering ability is strong, pollen amount foot, the easily production of hybrid seeds.
Accompanying drawing be in 413 family trees.
Embodiment: in the main seeding techniques of 413 series:
(1), proper seeding, guarantee flower synchronization.
In Hangzhou summer system, in " three is poor " indexs of 413 series of combination be:
Maternal date of seeding of male parent date of seeding, broadcast the poor leaf difference-product temperature difference
(moon/day) (moon/day) (my god) (leaf) (〉=10 ℃ of effective accumulated temperature) Xieyou 413 5/3 6/1 29 7.5 604.1 Shanyou 413s 5/3 6/5 33 8.0 691.2 Weiyou 413s 5/3 6/9 37 9.2 785.7
Various places can suitably be adjusted as the case may be
(2) father and mother's one's own profession ratio is 1: 10, male parent spacing in the rows 18cm, and maternal seeding row spacing is 11.5 * 12.5cm, strives for maternal every mu of effective fringe more than 200,000,20,000,000 above grain husks are spent number.
(3), fit and execute " GA ", the raising ripening rate
5~10% o'clock Zhenshan 97as of maternal heading and V20A spray " GA " 3 grams for every mu, the 2nd day 4 gram, the 3rd day 3 gram, Xieqingzao A gram on the same day 2, the 2nd day 3 gram, the 3rd day 2 gram.Carry out supplementary pollination with the bamboo bar.
(4) other management is identical with general high-yield seeds production field
In the main culture technique of 413 series of combination
1, proper seeding, seedling is strengthened in light seeding: it is identical with Shanyou 63 to do single late sowing phase, and it is identical with Shan excellent 10 to do two late sowing phases.
2, the transplanting of band tiller, rational close planting: individual plant band tiller is 2~3 during transplanting.The narrow strain plantation of wide row should be adopted 13.3 * 26.6cm or 11.7 * 30.0cm seeding row spacing.
3, heavy dressing base seed manure, control jointing fertilizer, the suitable ear manuer of executing, total amount of nitrogen is a little more than Shanyou 63.
4, science Guan Shui, early stage, shallow water inspired, and the middle and later periods does wet, and the strong bar of short root is protected the anti-early ageing of leaf.
In Other Main Agronomic Characters (1993) test card of 413 series of combination different locations and Ji Bie plantation
The effective fringe number of grain per ear of combination plant height mu in the other time of infertility in place season ripening rate thousand kernel weight per mu yield ± CK
( ) ( cm ) ( ) ( ) ( % ) ( g ) ( Kg ) ( % ) 413 143 114 20.22 145.2 90.15 27.6 678.8 +17.0363 ( CK ) 144 114 18.42 115.2 93.19 29.1 580413 139 110 16.47 150.66 85.29 28.2 564.08 +38.6110 ( CK ) 135 105 16.78 118.8 88.36 26.7 406.94413 140 115 17.25 156.28 88.38 28.2 620.0 +7.0063 ( CK ) 144 114 18.42 115.2 93.19 29.1 580413 135 101 15.42 176.2 87.4 28.9 651.7 +16.2710 ( CK ) 134 98 20.56 118.0 88.5 27.2 560.5413 131 84.2 20.33 145.59 68.78 27.65 550.33 +8.1410 ( CK ) 129 89.8 21.46 108.82 82.96 26.39 509.09413 130 95.8 18.97 133.71 80.44 26.57 532.5 +5.6810 ( CK ) 129 90.7 21.73 109.49 83.72 27.10 603.89
Claims (1)
1, the breeding method of a kind of hybrid rice, comprise proper seeding, rationally be with tiller transplanting, rational close planting, suitable row than and suitablely execute " GA " measure, it is characterized in that with samsara 422 be male parent, to select 10-19 is hybridization of female parent, seed selection wide-congenial restoring line 50465, as selecting index, 413 strains are in the acquisition with the performance of the advantage of hybrid F1 and field resistance situation; Is maternal with the Xieqingzao A, in 413 make up Xieyou 413 for the male parent preparing hybrid; Is maternal with the Zhenshan 97a, in 413 make up Shanyou 413 for the male parent preparing hybrid; Is maternal with the Wei 20A, in 413 make up Weiyou 413 for the male parent preparing hybrid; Is maternal with the Maxie A, in 413 make up Maxieyou 413 for the male parent preparing hybrid.
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CN 94102551 CN1037483C (en) | 1994-03-18 | 1994-03-18 | Method for breeding of cross-breeding rice |
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CN1037483C true CN1037483C (en) | 1998-02-25 |
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Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1166270A (en) * | 1997-04-28 | 1997-12-03 | 中国水稻研究所 | Production method of hybrid rice seed |
CN1077765C (en) * | 1998-05-12 | 2002-01-16 | 四川省农业科学院作物研究所 | Rice wide-congenial restoring line and its heterozygotic combination breeding method |
CN100397977C (en) * | 2006-08-17 | 2008-07-02 | 廖富东 | Breed method for rice reproduction heterosis fixation |
CN102771385A (en) * | 2012-08-22 | 2012-11-14 | 江苏丰源种业有限公司 | Breeding method for cultivating indica type three-line strong high-quality restoring line in multiple tiller positions |
CN103380726A (en) * | 2013-07-25 | 2013-11-06 | 巢湖春友种业科技有限公司 | Optional combination method utilizing Xieqing Zao A to perform test cross on a plurality of male parents |
CN111264322B (en) * | 2020-03-27 | 2022-01-21 | 四川农业大学 | Method for judging flower-season crossing of hybrid rice parents in mechanical transplanting seed production based on poor planting period |
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