CN107746395A - A kind of method of andrographolide in extraction chuanxinlian tablet - Google Patents

A kind of method of andrographolide in extraction chuanxinlian tablet Download PDF

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Publication number
CN107746395A
CN107746395A CN201710990295.5A CN201710990295A CN107746395A CN 107746395 A CN107746395 A CN 107746395A CN 201710990295 A CN201710990295 A CN 201710990295A CN 107746395 A CN107746395 A CN 107746395A
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China
Prior art keywords
andrographolide
extraction
chuanxinlian
gained
tablet
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Pending
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CN201710990295.5A
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Chinese (zh)
Inventor
张建会
杨东生
吴丽艳
秦书芝
耿璐璐
赵曦
孟凡欣
赵成国
周慧
李晶莹
吴威
滕利荣
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Zhuhai College of Jilin University
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Zhuhai College of Jilin University
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Priority to CN201710990295.5A priority Critical patent/CN107746395A/en
Publication of CN107746395A publication Critical patent/CN107746395A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
    • C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
    • C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D307/60—Two oxygen atoms, e.g. succinic anhydride
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Preparation (AREA)

Abstract

The invention discloses a kind of method for extracting andrographolide in chuanxinlian tablet, comprise the following steps：After taking appropriate chuanxinlian tablet to crush, it is placed in enzymatic vessel, adds complex enzyme in matter ratio, after vortex mixed, 1 2h is digested at 30 50 DEG C, filters, obtains enzymolysis liquid；By naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate age of starch in extract；Supernatant is collected, remainder centrifugation is collected centrifugal liquid and is incorporated in supernatant, is spray-dried, and the powder of gained is carried out into CO2Supercritical extract, fluid extraction pressure are 20 30MPa, 40 50 DEG C of extraction temperature, CO2Fluid flow is 750 850L/h, extracts 3 5h, collects andrographolide, produces.The present invention realizes the efficient extraction of andrographolide from chuanxinlian tablet, and easy to operate, while impurity in extract solution is not increased, without using the equipment of costliness, reduces the extraction cost of andrographolide.

Description

A kind of method of andrographolide in extraction chuanxinlian tablet
Technical field
The present invention relates to chemical extraction field, and in particular to a kind of method for extracting andrographolide in chuanxinlian tablet.
Background technology
Chuanxinlian tablet is heat-clearing formula, has the effect of clearing heat and detoxicating, cool blood detumescence.Cure mainly in evil poison and contain, cat fever, pharynx Larynx swells and ache, aphthae, pertussis overstrain cough, dysentery, puckery pain of heat gonorrhea, carbuncle swells sore, venomous snake bite.Its main component is punching Lotus, auxiliary material are starch, magnesium stearate.There are four kinds of Ginkgolide Components in Herba Andrographitis：It is deoxyandrographolide, andrographolide, new Andrographolide and Dehydro and drographolide.Andrographolide and total lactones are the clinical compositions generally used.Its main pharmacological It is antiallergy, anti HIV-1 virus, anti-inflammatory and anti-infectious function to learn activity.
At present, on the more of andrographolide extracting method report, water extraction, alcohol extracting method, buck extraction are specifically included Method etc., these technological operations are simple, but have that extraction efficiency is low, raw material utilization rate is low, solvent usage amount is big and impurity is difficult The problems such as removal, and other extracting methods, such as the extraction of supercritical extraction, ultrasonic assistant, microwave radiation exaraction, resin suction Attached method etc., although the higher andrographolide of purity can be made, required equipment is expensive, substantially increases production cost, And it is dfficult to apply to industrialization large-scale production.
The content of the invention
To solve the above problems, the invention provides a kind of method for extracting andrographolide in chuanxinlian tablet, from punching The efficient extraction of andrographolide is realized in lotus piece, and it is easy to operate, while impurity in extract solution is not increased, without making With the equipment of costliness, the extraction cost of andrographolide is reduced.
To achieve the above object, the technical scheme taken of the present invention is：
The method of andrographolide, comprises the following steps in a kind of extraction chuanxinlian tablet：
S1, after taking appropriate chuanxinlian tablet to crush, it is placed in enzymatic vessel, adds complex enzyme, whirlpool by mass percentage for 1-2% Rotation mixes 1-5min, and 1-2h is digested at 30-50 DEG C, filters, obtains enzymolysis liquid；
S2, by naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate age of starch in extract；Collect supernatant Liquid, remainder centrifugation, collect centrifugal liquid and be incorporated in supernatant, be spray-dried, obtain powder；
S3, the powder progress CO by gained2Supercritical extract, fluid extraction pressure are 20-30MPa, extraction temperature 40-50 DEG C, CO2Fluid flow is 750-850L/h, extraction time 3-5h, collects andrographolide, produces.
Preferably, the complex enzyme is mixed by acidic cellulase and acidic xylanase.
Preferably, the mass ratio of acidic cellulase and acidic xylanase is 2 in the complex enzyme:3.
Preferably, comprise the following steps：
S1, after taking appropriate chuanxinlian tablet to crush, it is placed in enzymatic vessel, adds complex enzyme, whirlpool by mass percentage for 1-2% Rotation mixes 3min, and 1.5h is digested at 35 DEG C, filters, obtains enzymolysis liquid；
S2, by naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate age of starch in extract；Collect supernatant Liquid, remainder centrifugation, collect centrifugal liquid and be incorporated in supernatant, be spray-dried, obtain powder；
S3, the powder progress CO by gained2Supercritical extract, fluid extraction pressure are 25MPa, 45 DEG C of extraction temperature, CO2 Fluid flow is 800L/h, extraction time 4h, is produced.
Preferably, the particle diameter after the chuanxinlian tablet crushes is the mesh of 40 mesh -60.
The invention has the advantages that：
The present invention enters first with acidic cellulase and acidic xylanase complex enzyme to the cell wall substance of Herba Andrographitis Row degraded, changes the permeability of cell membrane, promotes the dissolution of andrographolide, and dissolution time is short, and does not increase impurity, profit In subsequently using CO2Supercritical extract.Test and show in the andrographolide finished product as made from technical scheme, punching The content of lotus is higher.In addition, enzyme digestion reaction need not be very high temperature, avoid that andrographolide is thermo-labile, and high temperature easily decomposes The problem of loss.In addition, by enzymolysis pretreatment and CO2The impurity of the extract solution of andrographolide after supercritical extract processing Also it is not significantly increased, is easy to follow-up and isolates and purifies.And technical scheme does not need expensive equipment, reduces Equipment cost, and then reduce production cost.Acidic cellulase, acidic xylanase and Herba Andrographitis are filled by being vortexed Digested again after dividing mixing, further promote the dissolution of andrographolide.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair It is bright.
The mass ratio of acidic cellulase and acidic xylanase is 2 in complex enzyme used in following examples:3； Particle diameter after chuanxinlian tablet crushes is the mesh of 40 mesh -60.
Embodiment 1
The method of andrographolide, comprises the following steps in a kind of extraction chuanxinlian tablet：
S1, after taking appropriate chuanxinlian tablet to crush, it is placed in enzymatic vessel, adds complex enzyme, whirlpool by mass percentage for 1-2% Rotation mixes 3min, and 1.5h is digested at 35 DEG C, filters, obtains enzymolysis liquid；
S2, by naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate age of starch in extract；Collect supernatant Liquid, remainder centrifugation, collect centrifugal liquid and be incorporated in supernatant, be spray-dried, obtain powder；
S3, the powder progress CO by gained2Supercritical extract, fluid extraction pressure are 25MPa, 45 DEG C of extraction temperature, CO2 Fluid flow is 800L/h, extraction time 4h, collects andrographolide, produces.
Embodiment 2
The method of andrographolide, comprises the following steps in a kind of extraction chuanxinlian tablet：
S1, after taking appropriate chuanxinlian tablet to crush, it is placed in enzymatic vessel, adds complex enzyme, whirlpool by mass percentage for 1-2% Rotation mixes 1min, and 1h is digested at 50 DEG C, filters, obtains enzymolysis liquid；
S2, by naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate age of starch in extract；Collect supernatant Liquid, remainder centrifugation, collect centrifugal liquid and be incorporated in supernatant, be spray-dried, obtain powder；
S3, the powder progress CO by gained2Supercritical extract, fluid extraction pressure are 20MPa, 40 DEG C of extraction temperature, CO2 Fluid flow is 750L/h, extraction time 5h, collects andrographolide, produces.
Embodiment 3
The method of andrographolide, comprises the following steps in a kind of extraction chuanxinlian tablet：
S1, after taking appropriate chuanxinlian tablet to crush, it is placed in enzymatic vessel, adds complex enzyme, whirlpool by mass percentage for 1-2% Rotation mixes 5min, and 2h is digested at 30 DEG C, filters, obtains enzymolysis liquid；
S2, by naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate age of starch in extract；Collect supernatant Liquid, remainder centrifugation, collect centrifugal liquid and be incorporated in supernatant, be spray-dried, obtain powder；
S3, the powder progress CO by gained2Supercritical extract, fluid extraction pressure are 30MPa, 50 DEG C of extraction temperature, CO2 Fluid flow is 850L/h, extraction time 3h, collects andrographolide, produces.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should It is considered as protection scope of the present invention.

Claims (5)

  1. A kind of 1. method for extracting andrographolide in chuanxinlian tablet, it is characterised in that comprise the following steps：
    S1, after taking appropriate chuanxinlian tablet to crush, it is placed in enzymatic vessel, adds complex enzyme by mass percentage for 1-2%, is vortexed mixed 1-5min is closed, 1-2h is digested at 30-50 DEG C, filters, obtains enzymolysis liquid；
    S2, by naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate age of starch in extract；Collect supernatant, Remainder centrifuges, and collects centrifugal liquid and is incorporated in supernatant, is spray-dried, obtains powder；
    S3, the powder progress CO by gained2Supercritical extract, fluid extraction pressure are 20-30MPa, 40-50 DEG C of extraction temperature, CO2Fluid flow is 750-850L/h, extraction time 3-5h, collects andrographolide, produces.
  2. 2. a kind of method for extracting andrographolide in chuanxinlian tablet as claimed in claim 1, it is characterised in that described compound Enzyme is mixed by acidic cellulase and acidic xylanase.
  3. 3. a kind of method for extracting andrographolide in chuanxinlian tablet as claimed in claim 2, it is characterised in that described compound The mass ratio of acidic cellulase and acidic xylanase is 2 in enzyme:3.
  4. 4. a kind of method for extracting andrographolide in chuanxinlian tablet as claimed in claim 1, it is characterised in that including as follows Step：
    S1, after taking appropriate chuanxinlian tablet to crush, it is placed in enzymatic vessel, adds complex enzyme by mass percentage for 1-2%, is vortexed mixed 3min is closed, 1.5h is digested at 35 DEG C, filters, obtains enzymolysis liquid；
    S2, by naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate age of starch in extract；Collect supernatant, Remainder centrifuges, and collects centrifugal liquid and is incorporated in supernatant, is spray-dried, obtains powder；
    S3, the powder progress CO by gained2Supercritical extract, fluid extraction pressure are 25MPa, 45 DEG C of extraction temperature, CO2Fluid Flow is 800L/h, extraction time 4h, is produced.
  5. A kind of 5. method for extracting andrographolide in chuanxinlian tablet as claimed in claim 1, it is characterised in that the punching Particle diameter after lotus piece crushes is the mesh of 40 mesh -60.
CN201710990295.5A 2017-10-23 2017-10-23 A kind of method of andrographolide in extraction chuanxinlian tablet Pending CN107746395A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109512935A (en) * 2018-11-29 2019-03-26 四川旭阳药业有限责任公司 A kind of Qinghuozhimaipian and preparation method thereof
CN112033950A (en) * 2020-09-10 2020-12-04 青海省农林科学院 Pesticide residue detection method
CN112979594A (en) * 2021-02-26 2021-06-18 南通科鑫超临界设备有限公司 Supercritical extraction process of andrographolide

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1318398A (en) * 2000-04-19 2001-10-24 广州市轻工研究所 Method of extracting effective component of Herba Andrographitis
CN104529954A (en) * 2014-12-11 2015-04-22 李宏 Method for extracting andrographolide from common andrographis herb and andrographolide finished products
CN106501407A (en) * 2016-11-02 2017-03-15 百奥森（江苏）食品安全科技有限公司 The detection method of active constituent content in a kind of chuanxinlian tablet
CN106563001A (en) * 2016-10-12 2017-04-19 邯郸学院 Extraction method of plant active ingredients

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1318398A (en) * 2000-04-19 2001-10-24 广州市轻工研究所 Method of extracting effective component of Herba Andrographitis
CN104529954A (en) * 2014-12-11 2015-04-22 李宏 Method for extracting andrographolide from common andrographis herb and andrographolide finished products
CN106563001A (en) * 2016-10-12 2017-04-19 邯郸学院 Extraction method of plant active ingredients
CN106501407A (en) * 2016-11-02 2017-03-15 百奥森（江苏）食品安全科技有限公司 The detection method of active constituent content in a kind of chuanxinlian tablet

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
广东省中等医药专业教材编写组: "《药剂学实验指导（二年制药剂专业试用教材）》", 31 December 1974 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109512935A (en) * 2018-11-29 2019-03-26 四川旭阳药业有限责任公司 A kind of Qinghuozhimaipian and preparation method thereof
CN109512935B (en) * 2018-11-29 2021-08-24 四川旭阳药业有限责任公司 Cape jasmine-ophiopogon root tablet for clearing internal heat and preparation method thereof
CN112033950A (en) * 2020-09-10 2020-12-04 青海省农林科学院 Pesticide residue detection method
CN112979594A (en) * 2021-02-26 2021-06-18 南通科鑫超临界设备有限公司 Supercritical extraction process of andrographolide
CN112979594B (en) * 2021-02-26 2021-12-14 南通科鑫超临界设备有限公司 Supercritical extraction process of andrographolide

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