CN107723225A - A kind of classification inoculation apparatus and inoculation method - Google Patents
A kind of classification inoculation apparatus and inoculation method Download PDFInfo
- Publication number
- CN107723225A CN107723225A CN201711122592.4A CN201711122592A CN107723225A CN 107723225 A CN107723225 A CN 107723225A CN 201711122592 A CN201711122592 A CN 201711122592A CN 107723225 A CN107723225 A CN 107723225A
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- Prior art keywords
- inoculation
- air inlet
- bottle
- discharging opening
- lid
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- 238000011081 inoculation Methods 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000007599 discharging Methods 0.000 claims abstract description 24
- 229920001296 polysiloxane Polymers 0.000 claims abstract description 13
- 238000000855 fermentation Methods 0.000 claims description 34
- 230000004151 fermentation Effects 0.000 claims description 33
- 230000001954 sterilising effect Effects 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims 1
- 238000010276 construction Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of classification inoculation apparatus and inoculation method, including being inoculated with bottle, inoculation bottle top is provided with the lid being mutually matched, the inoculation bottle top is provided with rubber cushion, inoculation bottle passes through locked clip device with lid, lid is provided with charging aperture, air inlet and discharging opening, air inlet pipe is connected with air inlet, air inlet pipe further connects air cleaner, discharge nozzle is connected with discharging opening, the other end connection discharging opening interface of discharging opening, discharging opening bottom are connected with silicone tube, and silicone tube passes to inoculation bottle body bottom.Inoculation efficiency is high, effect of inoculation is good, safe and convenient to use, simple in construction, is easy to strength of adjustment, and convenient to repair, practicality is stronger.
Description
Technical field
The present invention relates to technical field of bioengineering, and in particular to a kind of classification inoculation apparatus and inoculation method.
Background technology
Fermentation tank is widely used in the industries such as dairy products, beverage, bioengineering, pharmacy, fine chemistry industry.For bioengineering
Fermentation requires that fermentation process is a sterile, free of contamination process.Fermentation tank employs sterile system, avoids and prevent air
The pollution of middle microorganism, substantially prolongs product shelf-life and product it is pure, be specifically designed on tank body and be mounted with sterile exhale
Suction hole or sterile malleation fermentation system.It is therefore more strict to sterility requirements in earlier fermentation especially strain seeded process,
Strain directly determines the asepsis pollution-free of the product of fermentation.Thus sterile inoculation method is that fermentation is successfully crucial.Infection
Miscellaneous bacteria easily lead to fermentation failure, but prior art still without can directly will ferment and inoculation be directly integrated set
It is standby.
The content of the invention
In view of the above-mentioned problems of the prior art, the present invention provides a kind of classification inoculation apparatus and inoculation method, inoculation efficiency
Height, effect of inoculation are good, safe and convenient to use, simple in construction, are easy to strength of adjustment, and convenient to repair, practicality is stronger.
To achieve these goals, the technical solution adopted by the present invention is：A kind of classification inoculation apparatus, including inoculation bottle, connect
Kind bottle top is provided with the lid being mutually matched, and the inoculation bottle top is provided with rubber cushion, and inoculation bottle passes through clip with lid
Locking, lid are provided with charging aperture, air inlet and discharging opening, are connected with air inlet pipe on air inlet, air inlet pipe further connects sky
Air filter, discharge nozzle is connected with discharging opening, the other end connection discharging opening interface of discharging opening, discharging opening bottom is connected with silicon
Sebific duct, silicone tube pass to inoculation bottle body bottom.Bottle is detachable is cleaned for inoculation, can using preceding by locked clip device lid
Carry out high pressure steam sterilization.
Preferably, the charging aperture is provided with charging lid.
Any of the above-described scheme is preferably, and the air inlet pipe connects air cleaner by one section of silicone tube.
Any of the above-described scheme is preferably, and the discharging opening connects discharging opening interface by one section of silicone tube.
Any of the above-described scheme is preferably, and the lid is stainless steel cover.
Any of the above-described scheme is preferably, the air inlet pipe, the both ends escape pipe corresponding with fermentation tank respectively of discharge nozzle
Road, charging aperture are connected.
The present invention also provides a kind of method being inoculated with using above-mentioned classification inoculation apparatus, comprises the following steps：
Step (1), the lid of bottle will be inoculated with locked clip device, air inlet pipe, discharge nozzle are wrapped up with the additional brown paper of gauze
It is good, it is put into sterilizing cabinet and is passed through high temperature and high pressure gas and is sterilized；
Closed after the completion of step (2), sterilizing and take out inoculation bottle and carry out high temperature drying, cooled down after drying standby；In nothing
Under bacterium operating environment, the charging aperture of inoculation bottle is opened, bacterium seed liquor is added into inoculation bottle, adds bonnet upper cover body and glue
Pad, and use locked clip device；
Step (3), will inoculation bottle be placed on by fermentation tank, by air inlet pipe connect fermentation tank admission line, discharge nozzle connect
Sending and receiving fermentation tank charging aperture, by fermentation tank pressure drop to 0.05Mpa, fermentation tank charging aperture valve is opened, by inoculation bottle
Body is pressurizeed, and strain is delivered into fermentation tank.
Any of the above-described scheme is preferably, and is also included after step (3)：After the completion of inoculation, fermentation tank charging aperture valve is closed
Door, air inlet pipe, the discharge nozzle of connection are taken out, dried being cleaned after inoculation bottle sterilization.
The beneficial effects of the invention are as follows：
1st, inoculation bottle is used to facilitate disassembly, cleaning for stainless steel, reuse and can be applicable in the present invention
In autoclave sterilization；
2nd, inoculation bottle air inlet is provided with air cleaner in the present invention, ability high-temperature sterilization, aperture 0.1-0.2 μm it
Between, microorganism can not be by that can be effectively ensured operating process sterile air and pass through；
3rd, inoculation method of the present invention is simple to operate, convenient efficiency high.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the preferred embodiment of classification inoculation apparatus one of the present invention；
Fig. 2 is Fig. 1 decomposing schematic representation.
Embodiment
The invention will be further described below in conjunction with the accompanying drawings.
To achieve these goals, the technical solution adopted by the present invention is：
As depicted in figs. 1 and 2, a kind of classification inoculation apparatus, including inoculation bottle 1, inoculation bottle 1 top are provided with what is be mutually matched
Lid 2, inoculation bottle 1 top are provided with rubber cushion 4, are inoculated with bottle 1 and are locked with the body of lid 2 by clip 3, lid 2 be provided with into
Material mouth 5, air inlet 6 and discharging opening 8, air inlet pipe 10 are connected with air inlet 6, air inlet pipe 10 further connects air cleaner 7.
Discharge nozzle 11 is connected with discharging opening 8, the other end connection discharging opening interface 9 of discharging opening 8, the bottom of discharging opening 8 connects
Silicone tube 12 is connected to, silicone tube 12 extends to inoculation bottle 1 bottom.Bottle 1 is detachable is cleaned for inoculation, passes through card before use
The locking lid of hoop 3, can carry out high pressure steam sterilization.
Charging aperture 5 is provided with charging lid 51.Air inlet pipe 10 connects air cleaner 7 by one section of silicone tube.Discharging opening 8
Discharging opening interface 9 is connected by one section of silicone tube.Lid 2 is stainless steel cover.Air inlet pipe 10, discharge nozzle 11 both ends respectively with hair
The corresponding outlet pipe of fermentation tank, charging aperture are connected.
The present invention also provides a kind of method being inoculated with using above-mentioned classification inoculation apparatus, comprises the following steps：
Step (1), by be inoculated with bottle 1 lid 2 locked with clip 3, air inlet pipe 10, discharge nozzle 11 are with the additional ox-hide of gauze
Paper bag ties, and is put into sterilizing cabinet and is passed through high temperature and high pressure gas and is sterilized；
Closed after the completion of step (2), sterilizing and take out inoculation bottle 1 and carry out high temperature drying, cooled down after drying standby；In nothing
Under bacterium operating environment, the charging aperture 5 of inoculation bottle 1 is opened, bacterium seed liquor is added into inoculation bottle 1, adds bonnet upper cover body 2
With rubber cushion 4, and locked with clip 3；
Step (3), will inoculation bottle 1 be placed on by fermentation tank, air inlet pipe 10 is connected into fermentation tank admission line, discharge nozzle
11 connection fermentation tank charging apertures, by fermentation tank pressure drop to 0.05Mpa, open fermentation tank charging aperture valve, pass through docking
Kind bottle is pressurizeed, and strain is delivered into fermentation tank.
After the completion of step (4), inoculation, fermentation tank charging aperture valve is closed, takes out air inlet pipe 10, the discharge nozzle 11 of connection,
Dried being cleaned after the inoculation sterilization of bottle 1.
The beneficial effects of the invention are as follows：
1st, inoculation bottle 1 is used to facilitate disassembly, cleaning for stainless steel, reuse and can be applicable in the present invention
In autoclave sterilization；
2nd, the inoculation air inlet 6 of bottle 1 is provided with the device of air filtration 7 in the present invention, is resistant to high-temperature sterilization, aperture is in 0.1-0.2 μ
Between m, microorganism can not be by that can be effectively ensured operating process sterile air and pass through；
3rd, inoculation method of the present invention is simple to operate, convenient efficiency high.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in,
It should all be included within the scope of the present invention.
Claims (7)
1. a kind of classification inoculation apparatus, including inoculation bottle, inoculation bottle top are provided with the lid being mutually matched, it is characterised in that institute
State inoculation bottle top and be provided with rubber cushion, with lid by locked clip device, lid is provided with charging aperture, air inlet and gone out inoculation bottle
Material mouth, air inlet pipe is connected with air inlet, air inlet pipe further connects air cleaner, is connected with discharge nozzle on discharging opening, goes out
The other end connection discharging opening interface of material mouth, discharging opening bottom are connected with silicone tube, and silicone tube passes to inoculation bottle body bottom.
2. a kind of classification inoculation apparatus according to claim 1, it is characterised in that the charging aperture is provided with charging lid.
3. a kind of classification inoculation apparatus according to claim 1, it is characterised in that the air inlet pipe is connected by one section of silicone tube
Air cleaner.
4. a kind of classification inoculation apparatus according to claim 1, it is characterised in that the discharging opening is connected by one section of silicone tube
Discharging opening interface.
5. a kind of classification inoculation apparatus according to claim 1, it is characterised in that the lid is stainless steel cover.
6. a kind of method that classification inoculation apparatus using described in any of the above-described claim is inoculated with, it is characterised in that including such as
Lower step：
Step (1), by be inoculated with bottle lid bandaged, put with the additional brown paper of gauze with locked clip device, air inlet pipe, discharge nozzle
Enter to be passed through high temperature and high pressure gas in sterilizing cabinet and sterilized；
Closed after the completion of step (2), sterilizing and take out inoculation bottle and carry out high temperature drying, cooled down after drying standby；In sterile behaviour
Make under environment, open the charging aperture of inoculation bottle, bacterium seed liquor is added into inoculation bottle, adds bonnet upper cover body and rubber cushion,
And use locked clip device；
Step (3), will inoculation bottle be placed on by fermentation tank, by air inlet pipe connect fermentation tank admission line, discharge nozzle connection hair
Fermentation tank charging aperture, by fermentation tank pressure drop to 0.05Mpa, fermentation tank charging aperture valve is opened, by entering to inoculation bottle
Row pressurization, fermentation tank is delivered to by strain.
7. fermentation tank sterile sampling method according to claim 6, it is characterised in that also include after step (3)：Inoculation
After the completion of, fermentation tank charging aperture valve is closed, takes out air inlet pipe, the discharge nozzle of connection, will be cleaned after inoculation bottle sterilization
Dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711122592.4A CN107723225A (en) | 2017-11-14 | 2017-11-14 | A kind of classification inoculation apparatus and inoculation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711122592.4A CN107723225A (en) | 2017-11-14 | 2017-11-14 | A kind of classification inoculation apparatus and inoculation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107723225A true CN107723225A (en) | 2018-02-23 |
Family
ID=61215511
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711122592.4A Pending CN107723225A (en) | 2017-11-14 | 2017-11-14 | A kind of classification inoculation apparatus and inoculation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107723225A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108285862A (en) * | 2018-04-18 | 2018-07-17 | 中国农业科学院农业资源与农业区划研究所 | Microbial ferment device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103382440A (en) * | 2013-06-24 | 2013-11-06 | 江苏元洲生物工程有限公司 | Safe inoculation device of liquid fermentation tank |
| CN204385199U (en) * | 2014-12-31 | 2015-06-10 | 镇江东方生物工程设备技术有限责任公司 | A kind of inoculating device of fermentation tank |
| CN106601458A (en) * | 2017-01-19 | 2017-04-26 | 厦门大互科技有限公司 | Mutual inductor pouring die |
-
2017
- 2017-11-14 CN CN201711122592.4A patent/CN107723225A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103382440A (en) * | 2013-06-24 | 2013-11-06 | 江苏元洲生物工程有限公司 | Safe inoculation device of liquid fermentation tank |
| CN204385199U (en) * | 2014-12-31 | 2015-06-10 | 镇江东方生物工程设备技术有限责任公司 | A kind of inoculating device of fermentation tank |
| CN106601458A (en) * | 2017-01-19 | 2017-04-26 | 厦门大互科技有限公司 | Mutual inductor pouring die |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108285862A (en) * | 2018-04-18 | 2018-07-17 | 中国农业科学院农业资源与农业区划研究所 | Microbial ferment device |
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| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180223 |
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| RJ01 | Rejection of invention patent application after publication |