CN107711507B - Plant tissue culture integrated device - Google Patents

Plant tissue culture integrated device Download PDF

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Publication number
CN107711507B
CN107711507B CN201711113930.8A CN201711113930A CN107711507B CN 107711507 B CN107711507 B CN 107711507B CN 201711113930 A CN201711113930 A CN 201711113930A CN 107711507 B CN107711507 B CN 107711507B
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China
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cylinder body
transparent cylinder
cover
cover plate
turnover
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CN201711113930.8A
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Chinese (zh)
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CN107711507A (en
Inventor
张君毅
黄锦飞
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Huaqiao University
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Huaqiao University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a plant tissue culture integrated device, which comprises an upper cover, a partition plate, a transparent cylinder body and a base, wherein the upper cover is provided with a groove; the top and the bottom of the transparent cylinder body are both provided with openings, and the upper cover is covered on the top of the transparent cylinder body; the partition plate is arranged at the joint of the upper cover and the transparent cylinder body and used for separating the upper cover and the transparent cylinder body; the base is arranged at the bottom of the transparent cylinder body and communicated with the transparent cylinder body; an LED lamp ring capable of illuminating the interior of the cylinder body is arranged at the upper edge of the transparent cylinder body; the partition plate, the cover frame and the turnover cover plate are enclosed to form a sterilization chamber; the partition plates comprise fixed partition plates and movable partition plates; the base is provided with a containing cavity for containing a culture medium, and the containing cavity is communicated with the transparent cylinder body.

Description

Plant tissue culture integrated device
Technical Field
The invention relates to a plant tissue culture integrated device.
Background
At present, many primary and secondary schools offer a plurality of science popularization education courses for plant tissue culture in order to enable students to better understand and learn plant tissue culture. Due to the influence of the current situation of the environment and the society, students are difficult to experience and perceive in the real environment of plant growth.
It is known that plant tissue culture requires collection, washing and sterilization, dedifferentiation and redifferentiation of plant tissue, and is performed in a laboratory by a technician. The method can be completed only by technical literacy in experimental environment and professions, has difficult operability for ordinary people, and cannot achieve intuitive and vivid science popularization education effect. The traditional plant tissue culture needs various procedures such as cultivation soil, fertilization and the like, the process is slow, the observation is difficult, and the aesthetic feeling of appreciation is difficult to achieve.
How to carry out tissue culture on common plants and simplify the plant tissue culture procedure, so that the plant tissue culture has visual and vivid science popularization effect and practical ornamental and practical value, namely the problem to be solved at present.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, provides the integrated plant tissue culture device, and solves the problems of science popularization, ornamental value and the like of plant tissue culture in the background technology.
The technical scheme adopted by the invention for solving the technical problems is as follows: provides a plant tissue culture integrated device, which comprises an upper cover, a clapboard, a transparent cylinder body and a base; the top and the bottom of the transparent cylinder body are both provided with openings, and the upper cover is covered on the top of the transparent cylinder body; the partition plate is arranged at the joint of the upper cover and the transparent cylinder body and used for separating the upper cover and the transparent cylinder body; the base is arranged at the bottom of the transparent cylinder body and communicated with the transparent cylinder body;
the upper cover comprises a cover frame, a turnover cover plate and a button; the cover frame is arranged at the upper part of the transparent cylinder body, an LED light source is arranged in the cover frame, the LED light source is arranged at the bottom of the cover frame in a surrounding manner, and a lamp ring capable of illuminating the inside of the cylinder body is formed at the upper edge of the transparent cylinder body; the turnover cover plate is arranged at the opening part of the cover frame, and the size of the turnover cover plate is matched with that of the opening part; the tail part of the turnover cover plate is hinged with one side of the cover frame; the tail end of the turnover cover plate is provided with a button, and the button is used for controlling the turnover cover plate to be opened and closed;
the partition plate, the cover frame and the turnover cover plate are surrounded to form a sterilization chamber; the partition plates comprise fixed partition plates and movable partition plates; the fixed clapboard is fixedly connected with the inner side of the cover frame, and a hollow part is arranged on the fixed clapboard; the movable partition board is pivoted on the fixed partition board, and the area of the movable cover board is larger than that of the hollow part;
the base is provided with a containing cavity for containing a culture medium, and the containing cavity is communicated with the transparent cylinder body.
In a preferred embodiment of the present invention, the hollow portion of the fixed partition is fan-shaped, the movable partition is fan-shaped and has the same center as the hollow portion, a rotating shaft is disposed at the center of the fan-shaped, and the movable partition is pivotally connected to the fixed partition through the rotating shaft.
In a preferred embodiment of the present invention, the edge of the cover frame is provided with a sliding groove; a shifting block is arranged at the arc position of the movable partition plate, penetrates through the sliding groove, is exposed out of the cover frame and is used for adjusting the relative position of the movable partition plate and the fixed partition plate; the hollow part is positioned in a fan-shaped range which is enclosed by taking the sliding groove as an arc length and taking the rotating shaft as a circle center.
In a preferred embodiment of the present invention, the cover frame is provided with an internal thread, the edge of the transparent cylinder body is provided with an external thread adapted to the internal thread, and the cover frame and the transparent cylinder body are connected by a thread.
In a preferred embodiment of the present invention, a spring is disposed at the lower portion of the button, and a clamping block is disposed at one side of the button close to the turnover cover plate; when the button and the turnover cover plate have continuous height, the fixture block is abutted against the turnover cover plate under the action of the spring, and the upper cover is closed; when the button is pressed down, the clamping block is far away from the turnover cover plate, the turnover cover plate is lifted, and the upper cover is opened.
In a preferred embodiment of the present invention, the transparent cylinder is a sphere with openings at the upper and lower parts after being cut in parallel; the upper cover is arched and is arranged at the upper cutting position; the base is an inverted round table and is arranged at a lower cutting position.
Compared with the background technology, the technical scheme has the following advantages:
1. the design of the spherical transparent cylinder body is convenient to observe, has great aesthetic feeling and ensures that the plant tissue culture process has ornamental value.
And 2, the LED light source provides sufficient and controllable light for the plants, so that the growth of the plants is controlled.
3. The use of the culture medium reduces stains, and is beautiful and convenient.
4. Be equipped with the sterile chamber in the upper cover, with the transparent cylinder body in lower part, base cooperation, reach plant tissue culture integration operation demand, reduce the operation degree of difficulty, increase audience crowd.
Drawings
FIG. 1 is a schematic view of the structure of the present invention.
Fig. 2 is a schematic view of the structure of the upper cover.
Fig. 3 is a sectional view of the upper cover.
Detailed Description
The invention is described in detail below with reference to the accompanying drawings:
referring to fig. 1-3, the integrated plant tissue culture device of the present embodiment includes an upper cover 1, a partition plate, a transparent cylinder 3 and a base 4; the top and the bottom of the transparent cylinder body 3 are both provided with openings, and the upper cover 1 covers the top of the transparent cylinder body 3; the partition plate is arranged at the joint of the upper cover 1 and the transparent cylinder body 3 and is used for separating the upper cover 1 and the transparent cylinder body 3; the base 4 is arranged at the bottom of the transparent cylinder 3 and communicated with the transparent cylinder 3; the transparent cylinder body 3 is a sphere with openings at the upper part and the lower part after parallel cutting; the upper cover 1 is arched and is arranged at an upper cutting position; the base 4 is an inverted round table and is arranged at a lower cutting position. The appearance of the whole integrated device is extremely beautiful, so that the plant tissue culture process is ornamental.
The upper cover 1 comprises a cover frame 11, a turnover cover plate 13 and a button 15; the cover frame 11 is arranged at the upper part of the transparent cylinder body 3, the LED light source 12 is arranged in the cover frame 11, the LED light source 12 is arranged at the bottom of the cover frame 11 in a surrounding manner, and a lamp ring capable of illuminating the inside of the cylinder body is formed at the upper edge of the transparent cylinder body 3; the turnover cover plate 13 is arranged at the opening part of the cover frame 11, and the size of the turnover cover plate is matched with that of the opening part; the tail part of the turnover cover plate 13 is hinged with one side of the cover frame 11; a button 15 is arranged at the tail end of the turnover cover plate 13, and the button 15 is used for controlling the opening and closing of the turnover cover plate 13;
the partition plate, the cover frame 11 and the turnover cover plate 13 are surrounded to form a sterilization chamber 5; the partition plates comprise a fixed partition plate 21 and a movable partition plate 23; the fixed clapboard 21 is fixedly connected with the inner side of the cover frame 11, and a hollow part is arranged on the fixed clapboard 21; the movable clapboard 23 is pivoted on the fixed clapboard 21, and the area of the movable cover plate is larger than that of the hollow part;
the base 4 is provided with a containing cavity 41 for containing a culture medium, and the containing cavity 41 is communicated with the transparent cylinder body 3.
In this embodiment, the hollow portion of the fixed partition 21 is fan-shaped, the movable partition 23 is fan-shaped and has the same center as the hollow portion, a rotating shaft 25 is disposed at the center of the fan-shaped circle, and the movable partition 23 is pivotally connected to the fixed partition 21 through the rotating shaft 25. The edge of the cover frame 11 is provided with a sliding chute 14; a shifting block 24 is arranged at the arc position of the movable partition plate 23, and the shifting block 24 passes through the sliding groove 14 to be exposed outside the cover frame 11 and is used for adjusting the relative position of the movable partition plate 23 and the fixed partition plate 21; the hollow part is positioned in a fan-shaped range enclosed by taking the sliding groove 14 as an arc length and the rotating shaft 25 as a circle center.
For the stability of guaranteeing to connect between lid frame 11 and transparent cylinder body 3, lid frame 11 is equipped with the internal thread, the edge of transparent cylinder body 3 be equipped with the external screw thread of internal thread looks adaptation, pass through threaded connection between lid frame 11 and transparent cylinder body 3.
A spring 16 is arranged at the lower part of the button 15, and a clamping block 18 is arranged at one side of the button 15 close to the turnover cover plate 13; when the button 15 and the turnover cover plate 13 have continuous height, the fixture block 18 is abutted against the turnover cover plate 13 due to the action of the spring 16, and the upper cover 1 is closed; when the button 15 is pressed down, the latch 18 moves away from the flip cover 13, the flip cover 13 is lifted, and the upper cover 1 is opened.
During operation: firstly, pressing a button 15 to enable a turnover cover plate 13 to be bounced open, putting cleaned plant tissues into a sterilization chamber 5, adding a proper amount of 70% alcohol, covering the turnover cover plate 13 to perform sterilization operation for 10-30 seconds, and pouring out redundant alcohol from a gap of the turnover cover plate 13; then, the poking block 24 is poked to adjust the relative position of the partition plate, so that the plant tissue falls into the base 4 filled with the MS culture medium; finally, the LED light source 12 is turned on, and tissue culture can be performed.
The whole growth process of the tissue culture can be observed from the ornamental jar. When plant tissue grows to the seedling or the device can't hold, can open the device, take out the seedling and transfer to other culture mediums or cultivate in the soil, wash this device, supply to use next time.
The above description is only a preferred embodiment of the present invention, and therefore should not be taken as limiting the scope of the invention, which is defined by the appended claims and their equivalents.

Claims (4)

1. The utility model provides a plant tissue culture integrated device which characterized in that: comprises an upper cover, a clapboard, a transparent cylinder body and a base; the top and the bottom of the transparent cylinder body are provided with openings, and the upper cover covers the top of the transparent cylinder body; the partition plate is arranged at the joint of the upper cover and the transparent cylinder body and used for separating the upper cover and the transparent cylinder body; the base is arranged at the bottom of the transparent cylinder body and communicated with the transparent cylinder body;
the upper cover comprises a cover frame, a turnover cover plate and a button; the cover frame is arranged at the upper part of the transparent cylinder body, an LED light source is arranged in the cover frame, the LED light source is arranged at the bottom of the cover frame in a surrounding manner, and a lamp ring capable of illuminating the inside of the cylinder body is formed at the upper edge of the transparent cylinder body; the turnover cover plate is arranged at the opening part of the cover frame, and the size of the turnover cover plate is matched with that of the opening part; the tail part of the turnover cover plate is hinged with one side of the cover frame; the tail end of the turnover cover plate is provided with a button, and the button is used for controlling the turnover cover plate to be opened and closed;
the partition plate, the cover frame and the turnover cover plate are surrounded to form a sterilization chamber; the partition plates comprise fixed partition plates and movable partition plates; the fixed clapboard is fixedly connected with the inner side of the cover frame, and a hollow part is arranged on the fixed clapboard; the movable partition board is pivoted on the fixed partition board, and the area of the movable cover board is larger than that of the hollow part;
the base is provided with an accommodating cavity for accommodating a culture medium, and the accommodating cavity is communicated with the transparent cylinder body;
the movable partition board is in a fan shape with the same circle center as the hollow part, a rotating shaft is arranged at the circle center of the fan shape, and the movable partition board is pivoted with the fixed partition board through the rotating shaft;
the edge of the cover frame is provided with a sliding chute; a shifting block is arranged at the arc position of the movable partition plate, penetrates through the sliding groove, is exposed out of the cover frame and is used for adjusting the relative position of the movable partition plate and the fixed partition plate; the hollow part is positioned in a fan-shaped range which is enclosed by taking the sliding groove as an arc length and taking the rotating shaft as a circle center.
2. The integrated plant tissue culture device according to claim 1, wherein: the cover frame is provided with an internal thread, the edge of the transparent cylinder body is provided with an external thread matched with the internal thread, and the cover frame is connected with the transparent cylinder body through the thread.
3. The integrated plant tissue culture device according to claim 2, wherein: a spring is arranged at the lower part of the button, and a clamping block is arranged at one side of the button close to the turnover cover plate; when the button and the turnover cover plate have continuous height, the fixture block is abutted against the turnover cover plate under the action of the spring, and the upper cover is closed; when the button is pressed down, the clamping block is far away from the turnover cover plate, the turnover cover plate is lifted, and the upper cover is opened.
4. An integrated plant tissue culture device according to any one of claims 1 to 3, wherein: the transparent cylinder body is a sphere with openings at the upper part and the lower part after parallel cutting; the upper cover is arched and is arranged at the upper cutting position; the base is an inverted round table and is arranged at a lower cutting position.
CN201711113930.8A 2017-11-13 2017-11-13 Plant tissue culture integrated device Active CN107711507B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711113930.8A CN107711507B (en) 2017-11-13 2017-11-13 Plant tissue culture integrated device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711113930.8A CN107711507B (en) 2017-11-13 2017-11-13 Plant tissue culture integrated device

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Publication Number Publication Date
CN107711507A CN107711507A (en) 2018-02-23
CN107711507B true CN107711507B (en) 2022-09-30

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004113160A (en) * 2002-09-27 2004-04-15 Toorin:Kk Plant raising apparatus by led (light emitting diode) light source
CN201178618Y (en) * 2008-03-07 2009-01-14 中国农业科学院农业环境与可持续发展研究所 Transparent container with luminous source
JP3158290U (en) * 2010-01-11 2010-03-25 スタンレー電気株式会社 Plant breeding appreciation device
WO2012077980A2 (en) * 2010-12-07 2012-06-14 (주)루트로닉 Stem cell culturing apparatus
CN107260004A (en) * 2017-07-27 2017-10-20 宁波弗兰克电器有限公司 A kind of pot lid and insulating pot
CN207589748U (en) * 2017-11-13 2018-07-10 华侨大学 Plant Tissue Breeding integrated apparatus

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004113160A (en) * 2002-09-27 2004-04-15 Toorin:Kk Plant raising apparatus by led (light emitting diode) light source
CN201178618Y (en) * 2008-03-07 2009-01-14 中国农业科学院农业环境与可持续发展研究所 Transparent container with luminous source
JP3158290U (en) * 2010-01-11 2010-03-25 スタンレー電気株式会社 Plant breeding appreciation device
WO2012077980A2 (en) * 2010-12-07 2012-06-14 (주)루트로닉 Stem cell culturing apparatus
CN107260004A (en) * 2017-07-27 2017-10-20 宁波弗兰克电器有限公司 A kind of pot lid and insulating pot
CN207589748U (en) * 2017-11-13 2018-07-10 华侨大学 Plant Tissue Breeding integrated apparatus

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