CN205105914U - Plant tissue culture bottle - Google Patents
Plant tissue culture bottle Download PDFInfo
- Publication number
- CN205105914U CN205105914U CN201520806329.7U CN201520806329U CN205105914U CN 205105914 U CN205105914 U CN 205105914U CN 201520806329 U CN201520806329 U CN 201520806329U CN 205105914 U CN205105914 U CN 205105914U
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- bottle
- led
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- tissue culture
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- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 15
- 239000011521 glass Substances 0.000 claims abstract description 24
- 238000009434 installation Methods 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 abstract description 6
- 239000000428 dust Substances 0.000 abstract description 6
- 239000007787 solid Substances 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 4
- 238000009630 liquid culture Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 238000002834 transmittance Methods 0.000 description 3
- 241001313857 Bletilla striata Species 0.000 description 2
- 235000005976 Citrus sinensis Nutrition 0.000 description 2
- 240000002319 Citrus sinensis Species 0.000 description 2
- 240000001131 Nostoc commune Species 0.000 description 2
- 235000013817 Nostoc commune Nutrition 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a plant tissue culture bottle belongs to plant group and banks up technical field with earth. The body is a cylinder, and there is a recess on a bottle top surface, and inside the convex surface subtend bottle of recess, the LED lamp stand is buckled with the osculum on the space glass board mutually, and the degree of depth that the length of LED lamp is less than the recess is aimed at in the recess just to the LED lamp during installation, places space glass board on the bottle top, open body one side has the side bottleneck, and side bottleneck nose's outer wall is provided with the outer wall screw thread and matches with the inner wall of thread of bottle lid, and the board is cultivateed with body axial vertical and place the bottom in the body to the net. The utility model discloses effectively avoid bacterium, dust, debris etc. To fall into easily the circumstances that leads to the fact the pollution in the bottle perpendicularly to improvement group banks up seedling quality and survival rate of seedlings with earth, and effective drop set banks up the cost with earth, solved the solid culture and the liquid blake bottle can not share effectively, and the group banks up the seedling with earth and can not stand vertically, empty easily, washs the problem of difficulty, has fine application prospect.
Description
Technical field
The utility model relates to a kind of plant tissue culture bottle, belongs to plant tissue culture technical field.
Background technology
Plant tissue culture fast breeding technique has become one of the most important method of plant fast seedling growing, seed selection new varieties at present, by its growth conditions of Artificial Control in group training process, for it provides suitable growing environment.Illumination is the important factor of plant growth, adopts illumination more, but have that light source is single, power consumption is very large in existing group of training.Much research shows the elongation of the blue light ingredient prevention test-tube plantlet in spectrum, but can promote the synthesis of the formation of leaf and various photosynthetic pigment.Red-light LED contributes to increasing plant height, internode length and rooting rate, and blue light is relevant with the growth of chlorophyllous synthesis and pore, and the investigation test of spectrum is also more and more paid attention to.In tissue culture procedures, use open-topped traditional tissue culture bottle, when explant is implanted, extraneous bacterium, dust, foreign material etc. easily enter from bottleneck, easily pollute, and cause the failure tested; And Nostoc commune Vanch bottled enter liquid nutrient medium time, seedling can not be upright, or weak vibrations tissue culture bottle, plantlet in vitro is easily toppled over, there is solid culture and liquid culture bottle can not share, cause the waste of resource, add extra production cost, and the culture plate in Nostoc commune Vanch bottle can not take out, there is the problem of cleaning difficulty.
Therefore, based on above problem, the utility model, by bottleneck is moved to side, improves the light transmittance of tissue culture bottle, and lateral opening inoculation can avoid bacterium, dust, foreign material etc. vertically to fall into, and effectively reduces pollution rate.A groove is set at tissue culture bottle top, its size shape just can settle a LED, and the LED of different wave length can be changed as required at any time, meet the different demands of plant, also be conducive to process of the test and detect plant to the demand of lighting color, make the investigation of spectrum in group training test accurately more convenient.
Summary of the invention
In order to overcome the deficiency of traditional tissue culture flasks, illumination power consumption is large, causes the grid electricity fee cost of great number; The light source of different-waveband is investigated to the impact of plant growth in local that can not be independent; The open-topped tissue culture bottle of tradition, bacterium, dust, foreign material etc. are easily vertically fallen in bottle, cause a large amount of pollution; Solid culture and liquid culture bottle can not share, and can not take out due to culture plate, and the problem of cleaning difficulty, the utility model provides a kind of plant tissue culture bottle.
The technical solution of the utility model is: a kind of plant tissue culture bottle, comprises body 1, groove 2, LED 3, space glass plate 4, LED seat 5, side bottleneck 6, bottle cap 7, outer wall thread 8, inner thread 9, grid culture plate 10;
Described body 1 is a cylinder, bottle top upper surface has a groove 2, the convex surface subtend bottle interior of groove 2, and LED seat 5 interlocks with the osculum on space glass plate 4, during installation, LED 3 is registered to groove 2 li and the length of LED 3 is less than the degree of depth of groove 2, and space glass plate 4 is placed on bottle top; Body 1 side has side bottleneck 6, and the outer wall of side bottleneck 6 extension is provided with outer wall thread 8 and matches with the inner thread 9 of bottle cap 7, the axially vertical and bottom be positioned in body 1 of grid culture plate 10 and body 1.
Described grid culture plate 10 adopts deformable colloidal materials to make.
The height of described grid culture plate 10 is 2cm.
Wherein, the body 1 of tissue culture bottle, the material of groove 2 adopt space glass to be main material, and light transmittance is strong; Groove 2 size shape just can lay a LED 3.
As: as described in tissue culture bottle body 1 be highly 12cm, diameter at the bottom of bottle is 8cm; Side bottleneck 6 angle of inclination is 45 °, side bottleneck 6 diameter 6cm; At the bottom of distance bottle, 3cm place arranges lateral opening 6(1/4 place at the bottom of distance bottle on body 1 and is provided with lateral opening 6).
Use procedure of the present utility model is:
Before using, this tissue culture bottle is split and clean up, body 1 is separated with LED 3, bottle cap 7 dismounting, and grid culture plate 10 is taken out; Then cleaned tissue culture bottle is put into oven for drying.Preparation culture fluid, left hand arrests tissue culture bottle, and body 1 is tilted a little, and right handgrip prepares nutrient solution and slowly pours bottle into, and culture fluid just covers grid culture plate 10 and is advisable.Body side bottleneck outer wall thread 8 and bottle cap inner thread 9 screw, and put into high-pressure sterilizing pot and carry out sterilizing, temperature is set to 121 DEG C, and the time is 30min.The medium that sterilizing cools is implanted explant on aseptic operating platform, when side bottleneck 6 implants explant, bacterium, dust, foreign material etc. can be effectively avoided easily vertically to fall into situation about polluting in bottle, when liquid culture, grid culture plate 10 can well support long explant, such as, during the culture of rootage of bletilla striata seedling, bletilla striata seedling can well be supported, avoid seedling to tilt to pour liquid nutrient medium into, cause rotting of stem stalk, the situation such as to take root bad.After explant implantation, bottle cap 7 is burnt on alcolhol burner and screw with side bottleneck 6 again for several seconds.Finally blake bottle being taken out aseptic operating platform is placed on culturing rack, the LED lamp source of different-waveband is installed according to test requirements document, plant is the most responsive to the reaction of 400-510nm royal purple light section, 610-720nm blood orange light section and 720-780nm far-red light section, wherein absorbable wavelength mainly concentrates on royal purple light section (crest is 450nm) and blood orange light section (crest is 660nm), but the suitable light quality of different plant is all not quite similar.The utility model interlocks LED seat 5 and the osculum on space glass plate 4, allows LED 3 be registered to the groove 2 li at bottle top, space glass plate 4 is placed on bottle and pushes up during installation.The length of LED 3 is less than the degree of depth of groove 2.LED lamplight shines directly into plant by groove 2 convex surface, and LED is installed simple and convenient, and arbitrarily can change the LED lamp source of different wave length.Finally the blake bottle being provided with LED is placed on culturing rack carries out cultivate, observe, record.
The beneficial effects of the utility model are:
1, lateral opening, compare the existing bottle cap made with translucent opalescent plastic being placed in top, its light transmittance can increase by 20 ~ 30%, increases plantlet in vitro photosynthetic capacity; Effectively avoid bacterium, dust, foreign material etc. easily vertically to fall into situation about polluting in bottle, thus improve plantlet in vitro quality and transplanting survival rate, effective reduction group training cost.
2, external LED, need not put into high-pressure sterilizing pot sterilizing together with medium, arbitrarily can change the LED of different-waveband, can not pollute, and the LED of different-waveband is investigated to the impact of plant growth in local that can be independent.
3, soft grid culture plate, efficiently solves solid culture and liquid culture bottle can not share, and plantlet in vitro can not uprightly, easily be toppled over, and the problem of cleaning difficulty, has good application prospect.
Accompanying drawing explanation
Fig. 1 is the utility model assembly drawing one;
Fig. 2 is the utility model assembly drawing two;
Fig. 3 is profile of the present utility model;
Each label in figure: 1-body; 2-groove; 3-LED lamp; 4-space glass plate; 5-LED lamp socket; 6-side bottleneck; 7-bottle cap; 8-outer wall thread; 9 lid inner threads; 10-grid culture plate.
Embodiment
Embodiment 1: as Figure 1-3, a kind of plant tissue culture bottle, comprises body 1, groove 2, LED 3, space glass plate 4, LED seat 5, side bottleneck 6, bottle cap 7, outer wall thread 8, inner thread 9, grid culture plate 10;
Described body 1 is a cylinder, bottle top upper surface has a groove 2, the convex surface subtend bottle interior of groove 2, and LED seat 5 interlocks with the osculum on space glass plate 4, during installation, LED 3 is registered to groove 2 li and the length of LED 3 is less than the degree of depth of groove 2, and space glass plate 4 is placed on bottle top; Body 1 side has side bottleneck 6, and the outer wall of side bottleneck 6 extension is provided with outer wall thread 8 and matches with the inner thread 9 of bottle cap 7, the axially vertical and bottom be positioned in body 1 of grid culture plate 10 and body 1.
Described grid culture plate 10 adopts deformable colloidal materials to make.
The height of described grid culture plate 10 is 2cm.
Embodiment 2: as Figure 1-3, a kind of plant tissue culture bottle, comprises body 1, groove 2, LED 3, space glass plate 4, LED seat 5, side bottleneck 6, bottle cap 7, outer wall thread 8, inner thread 9, grid culture plate 10;
Described body 1 is a cylinder, bottle top upper surface has a groove 2, the convex surface subtend bottle interior of groove 2, and LED seat 5 interlocks with the osculum on space glass plate 4, during installation, LED 3 is registered to groove 2 li and the length of LED 3 is less than the degree of depth of groove 2, and space glass plate 4 is placed on bottle top; Body 1 side has side bottleneck 6, and the outer wall of side bottleneck 6 extension is provided with outer wall thread 8 and matches with the inner thread 9 of bottle cap 7, the axially vertical and bottom be positioned in body 1 of grid culture plate 10 and body 1.
Described grid culture plate 10 adopts deformable colloidal materials to make.
Embodiment 3: as Figure 1-3, a kind of plant tissue culture bottle, comprises body 1, groove 2, LED 3, space glass plate 4, LED seat 5, side bottleneck 6, bottle cap 7, outer wall thread 8, inner thread 9, grid culture plate 10;
Described body 1 is a cylinder, bottle top upper surface has a groove 2, the convex surface subtend bottle interior of groove 2, and LED seat 5 interlocks with the osculum on space glass plate 4, during installation, LED 3 is registered to groove 2 li and the length of LED 3 is less than the degree of depth of groove 2, and space glass plate 4 is placed on bottle top; Body 1 side has side bottleneck 6, and the outer wall of side bottleneck 6 extension is provided with outer wall thread 8 and matches with the inner thread 9 of bottle cap 7, the axially vertical and bottom be positioned in body 1 of grid culture plate 10 and body 1.
The height of described grid culture plate 10 is 2cm.
Embodiment 4: as Figure 1-3, a kind of plant tissue culture bottle, comprises body 1, groove 2, LED 3, space glass plate 4, LED seat 5, side bottleneck 6, bottle cap 7, outer wall thread 8, inner thread 9, grid culture plate 10;
Described body 1 is a cylinder, bottle top upper surface has a groove 2, the convex surface subtend bottle interior of groove 2, and LED seat 5 interlocks with the osculum on space glass plate 4, during installation, LED 3 is registered to groove 2 li and the length of LED 3 is less than the degree of depth of groove 2, and space glass plate 4 is placed on bottle top; Body 1 side has side bottleneck 6, and the outer wall of side bottleneck 6 extension is provided with outer wall thread 8 and matches with the inner thread 9 of bottle cap 7, the axially vertical and bottom be positioned in body 1 of grid culture plate 10 and body 1.
Composition graphs is explained in detail embodiment of the present utility model above, but the utility model is not limited to above-mentioned embodiment, in the ken that those of ordinary skill in the art possess, various change can also be made under the prerequisite not departing from the utility model aim.
Claims (3)
1. a plant tissue culture bottle, is characterized in that: comprise body (1), groove (2), LED (3), space glass plate (4), LED seat (5), side bottleneck (6), bottle cap (7), outer wall thread (8), inner thread (9), grid culture plate (10);
Described body (1) is a cylinder, bottle top upper surface has a groove (2), the convex surface subtend bottle interior of groove (2), LED seat (5) interlocks with the osculum on space glass plate (4), during installation, LED (3) is registered to the degree of depth that the inner and length that is LED (3) of groove (2) is less than groove (2), and space glass plate (4) is placed on bottle top; Body (1) side has side bottleneck (6), the outer wall of side bottleneck (6) extension is provided with outer wall thread (8) and matches with the inner thread (9) of bottle cap (7), the axially vertical and bottom be positioned in body (1) of grid culture plate (10) and body (1).
2. plant tissue culture bottle according to claim 1, is characterized in that: described grid culture plate (10) adopts deformable colloidal materials to make.
3. plant tissue culture bottle according to claim 1 and 2, is characterized in that: the height of described grid culture plate (10) is 2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520806329.7U CN205105914U (en) | 2015-10-19 | 2015-10-19 | Plant tissue culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520806329.7U CN205105914U (en) | 2015-10-19 | 2015-10-19 | Plant tissue culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205105914U true CN205105914U (en) | 2016-03-30 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520806329.7U Expired - Fee Related CN205105914U (en) | 2015-10-19 | 2015-10-19 | Plant tissue culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205105914U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110367122A (en) * | 2019-08-11 | 2019-10-25 | 华中农业大学 | A kind of tissue culture seedlings of potatoes culture box of suitable mechanization production |
-
2015
- 2015-10-19 CN CN201520806329.7U patent/CN205105914U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110367122A (en) * | 2019-08-11 | 2019-10-25 | 华中农业大学 | A kind of tissue culture seedlings of potatoes culture box of suitable mechanization production |
| CN110367122B (en) * | 2019-08-11 | 2022-05-27 | 华中农业大学 | Potato tissue culture seedling culture box suitable for mechanized production |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160330 Termination date: 20171019 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |