CN107711025A - Improve the fertilizing method of Bu Funa tender leaf anticancer functions - Google Patents
Improve the fertilizing method of Bu Funa tender leaf anticancer functions Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B13/00—Fertilisers produced by pyrogenic processes from phosphatic materials
- C05B13/02—Fertilisers produced by pyrogenic processes from phosphatic materials from rock phosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention belongs to planting technology field, more particularly to a kind of fertilizing method of raising Bu Funa tender leaf anticancer functions, including following preparation process：After Bu Funa insemination and emergences, herb liquid was sprayed every 5 days, each fountain height is 10 30ml, it is continuous to spray 46 times, afterwards, start out the deep annular grooves of 10 20cm around away from the 50cm of root 30, overburden soil after application rich water disk fertilizer, the fertilising in other stages are conventionally applied fertilizer.By fertilizing method of the present invention, the anticancer function of Bu Funa tender leafs is effectively improved.
Description
Technical field
The invention belongs to planting technology field, more particularly to a kind of fertilizing method of raising Bu Funa tender leaf anticancer functions.
Background technology
Bu Funa (Kadsura coccinea), the black tiger of scientific name, it is Schisandraceae (or Magnoliaceae) plant thickness leaf five tastes
The fruit of son, the evergreen liana of chain timbers matter is primary in Southwestern China portion virgin forest, is now difficult to find its trace.Miao " Bu Funa ",
Mean the fruit of beautifying and life-prolonging.Planted in recent years by introducing a fine variety examination in northern some areas, and turned into the new varieties that a seed selection forms,
To integrate novel fruits that are edible, medicinal, afforesting, view and admire, be that a urgency is to be developed and great exploitation potential it is multi-purpose
Way rare plant.Bu Funa is primary in virgin forest, is the fruit of Miao people's cultivation from generation to generation, and Miao is called " Bu Fu
Na ", mean the fruit of beautifying and life-prolonging.Root is medicinal, can the promoting flow of qi and blood circulation, swelling and pain relieving controls stomach trouble, treating rheumatic ostealgia, bruise stasis caused pain, and
For gynaecology's common medicine.It is sweet after fruit maturation, it is edible.Cosmetic raw materials can be used as, there is splendid whitening beauty, anti-aging
Effect, it is preferred in cosmetic industry, it, which changes a kind of posture, can appear in major beauty parlor, and cosmetics shop is expensive.Bu Fu
The tender leaf of Na can be used as health protection tea, often drink and anticancer function.And in the prior art, for how to improve Bu Funa tender leaf anticancers
The fertilizing method of effect, there is no people to study.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of fertilizing method of raising Bu Funa tender leaf anticancer functions.
To achieve the above object, the present invention adopts the following technical scheme that：
The present invention provides the fertilizing method for improving Bu Funa tender leaf anticancer functions, including following preparation process：Bu Funa is broadcast
After kind emergence, herb liquid was sprayed every 5 days, each fountain height is 10-30ml, continuous to spray 4-6 times, afterwards, away from root 30-
Start out the deep annular grooves of 10-20cm around 50cm, apply overburden soil after rich water disk fertilizer, the fertilising in other stages is according to routine
Method is applied fertilizer.
Rich water disk fertilizer of the present invention, is made up of the raw material of following parts by weight：Farm manure 80-120 parts, calcium and magnesium phosphorus are answered
Hefei 10-20 parts, traditional Chinese medicine powder 15-25 parts.
Traditional Chinese medicine powder of the present invention, it is made up of the raw material of following parts by weight：Dayflower 28-46 parts, brassica campestris L. seed 12-28
Part, rhizoma Gastrodiae 5-18 parts.
Herb liquid of the present invention, it is made up of the raw material of following parts by weight：Radix bupleuri 20-50 parts, asarum 5-35 parts, Jehol Ligusticum Rhizome
10-25 parts, dandelion 1-10 parts.
Preferably, rich water disk fertilizer of the present invention, is made up of the raw material of following parts by weight：100 parts of farm manure, calcium and magnesium
15 parts of phosphorus composite fertilizer, 20 parts of traditional Chinese medicine powder.
Preferably, traditional Chinese medicine powder of the present invention, it is made up of the raw material of following parts by weight：37 parts of dayflower, brassica campestris L. seed 20
Part, 12 parts of rhizoma Gastrodiae.
Preferably, herb liquid of the present invention, it is made up of the raw material of following parts by weight：35 parts of radix bupleuri, 20 parts of asarum, ligusticumic
This 18 parts, 5 parts of dandelion.
Preferably, farm manure of the present invention is animal wastes, stacks fermentation 10-15 days, is dried to obtain.
Preferably, animal wastes of the present invention are the one or more in chicken manure, duck excrement, pig manure.
Preferably, Chinese medicine powder, preparation method thereof of the present invention is：Traditional Chinese medicine powder whole raw material is well mixed, addition and Chinese medicine
The water of powder whole raw material equivalent weight, adds its weight 0.2-0.6% dusty yeasts, and ferment 20-32h at 30-35 DEG C, drying,
It is crushed to 150-250 mesh.
Preferably, the preparation method of herb liquid of the present invention is：Herb liquid whole raw material is well mixed, is crushed to
100-200 mesh, the water of whole herb liquid raw material equivalent weights is added, handle 8-10min with high-speed shearing emulsion machine, rotating speed is
7000-9000r/min, then under 48-62MPa pressure and handle 8- at a temperature of 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment
14min, number of processes are 2-3 times, obtain paste serous material；Paste serous material weight 0.3-0.8% dusty yeast is added, mixing is stirred
Mix uniformly, at 28-32 DEG C, be sealed by fermentation 30-42h, filtering, obtain filtered fluid, the water for adding 20-30 times of filtered fluid volume is dilute
Release, obtain herb liquid.
Preferably, it is 5-7kg that every plant of Bu Funa of the present invention, which needs rich water disk fertilizer,.
In the present invention, the conventional method fertilising refers to seedling half a year fertilising 1 time of not bearing fruit, based on nitrogenous fertilizer, after bearing fruit
Apply fertilizer 1 time within 10 days, based on phosphate fertilizer, potash fertilizer.
In the herb liquid raw material of the present invention：
Radix bupleuri, samphire radix bupleuri scorzoneraefolii Bupleurum scorzonerifolium Willd. root.Cold nature,
Bitter.Return liver warp, gallbladder channel, lung channel.This product is mainly containing valeric acid, leukotrienes, palmitic acid, stearic acid, Kaempferol, Kaempferitrin, quercitrin
Element, rutin sophorin, aromadendrene, borneol, bigleaf thorowax root saponin(e II, myrtanol, α-spinaterol, go glucosyl group saikoside α,
The compositions such as saikoside.The compositions such as the another alcohol of Fu Shou containing spring.Pharmacological testing proves, have anti-inflammatory, antipyretic, calm, analgesia, antibechic and
Anticonvulsant action；Hepatic injury can be mitigated and promote choleresis；With hypotensive, reduce serum cholesterol and haemocylolysis；
With effects such as fat content, Antiradiation injuries in antiulcer, antibacterial, antiviral, antitumor, rise blood glucose, reduction blood.
Asarum, aristolochiaceae plant asarum sieboldii Asarum sieboldii Miq. herb.It is warm-natured, acrid flavour.Thoughts of returning home warp,
Lung channel, kidney channel.Wind-dispelling, cold dispelling, sensible analgesic, warm lung eliminating the phlegm.Belong to the warm-pungent diaphoretic drug of diaphoretic medicine subordinate classification.Containing volatile oil,
Main component methyl eugenol, eucarvone, safrole, nopinene, australene, borneol, different anisole, asarum ester element etc..This
Outside, still containing a kind of racemization-remove potassium coclaurine.Trace element has potassium, sodium, magnesium, calcium, iron, manganese, copper, zinc etc..Separately containing Kaempferol, mountain
Naphthol 3-O- β-D- glucopyranosides, γ-terpinene, β-terpinene, α-thujene, asarone, (-)-asarinin etc. into
Point.There is local anaesthesia effect, there is a certain degree of inhibitory action to arthritis, also with certain bacteriostasis, there is enhancing fat
Matter is metabolized and the effect of rise blood glucose, adjusts body smooth muscle function.Volatile oil can make anesthetized animal drop in blood pressure, and decoction is then
Blood pressure is set to increase；And have anti-inflammatory and anticonvulsant action.Also analgesia, calm, suppression heating, antipyretic, antihistamine and resistance state are anti-
Should be with excited respiration.
Jehol Ligusticum Rhizome, samphire Jehol Ligusticum Rhizome Ligusticum sinense Oliv. rhizome and root.It is warm-natured, acrid flavour.Return wing
Guang passes through.Cold dispelling, dehumidifying, analgesic.Belong to the warm-pungent diaphoretic drug of diaphoretic medicine subordinate classification.Containing volatile oil 0.3%~0.65%, wherein newly
Cnidiumlactone content highest, accounts for 25.57%.Separately containing in forulic acid, aromadendrene, Bronyl acetate, australene, nopinene, Jehol Ligusticum Rhizome
The compositions such as ester, Senkyunolide A, senkyunolide G, Senkynolide H, senkyunolide Ⅰ, 3- butylidenephthalides.Water extract
Or alcohol extract has obvious antihypertensive effect, while blood vessel can also be directly expanded, it is excited to myenteron caused by anti-acetylcholine, hence it is evident that drop
Low in vitro rabbit uterus tension force, and excitation of the oxytocins to uterus can be resisted.Pharmacological evaluation shows there is significant calm, town
The central inhibitory actions such as bitterly, antipyretic and cooling.Also anti-inflammatory；Suppress smooth muscle；Improve hypoxia-bearing capability；With antibacterial, anti-liver
Infringement, Robust speaker feature, anti-oxidant etc. act on.
Dandelion, feverfew dandelion Taraxacum mongolicum Hand.-Mazz. whole herb with root.It is cold in nature,
It is bitter, sweet.Return liver warp, stomach.Containing taraxasterol, choline, synanthrin, pectin, dandelion sterol, taraxacin, dandelion match
The compositions such as alcohol, caffeic acid.Acted on resisting pathogenic microbes, antitumor, anti-gastric-ulcer, cholagogic and liver protection, it is direct during low concentration
Excited isolated frog heart, and during high concentration it is in then inhibitory action.The tonicity of Isolated Duodenum can be improved and strengthen its convergent force,
Clinic thinks there is stomach invigorating and laxative effective.Parenteral solution is in vitro to staphylococcus aureus resistance bacterial strain, hemolytic streptococcus
Have stronger bactericidal action, to Diplococcus pneumopniae, meningococcus, corynebacterium diphtheriae, Pseudomonas aeruginosa, proteus, shigella dysenteriae,
Typhoid bacillus and micrococcus catarrhalis also have certain bactericidal action.Toxicity：Small white mouse intravenous injection Dandelion Injectable Solution LDso be
(58.88 ± 7.94) g kg, mouse, rabbit subacute toxicity test may occur in which a small amount of cast, renal cells to kidney
Cloudy swelling ribbon, decoction absorb well to Oral Administration in Rats, certain antibacterial action can be kept in urine.
In the Chinese medicine powder raw material of the present invention：
Dayflower, commelianaceae plant dayflower Commelina communis L. aerial part.It is cold in nature, sweet,
It is light.Return lung warp, stomach, bladder warp.Clearing heat and detoxicating, inducing diuresis for removing edema.The heat-clearing and detoxifying drug classified under category antipyretic.Petal plantar containing duck
Straw colour ketoside, it is a kind of pigment of yellow.It is separately yellow containing left-handed-loliolide, freidelin, β-sitosterol, the blue element of duck plantar, duck plantar
The compositions such as ketoside, harman arabine.There are antibacterial, antiendotoxin and analgesic activity.The water of congener Commelina pallida cauline leafs
Preserved material or the excited uterus of decoction energy, vasoconstriction, and the clotting time can be shortened.
Brassica campestris L. seed, crucifer rape Brassica campesrtris L. dry mature seed.It is mild-natured, taste
It is pungent, sweet.Return liver warp, large intestine channel.Promoting blood circulation and removing blood stasis, dispersing swelling and dissipating binds, relax bowel.The promoting blood circulation for regulating menstruation classified under category drug for invigorating blood circulation and eliminating stasis
Medicine.The main mustard of turnip containing glucose element, the amyl- 4- alkene -ester of glucose isothiocyanic acid, preceding announcement Yichun, but fatty oil, protein,
Rutin sophorin, brassicasterol, 22- dehydrogenations campesterol and several amino acids.There is antibacterial action, have to gram-positive cocci necessarily
Inhibitory action, another this product oil mass containing plant is more, there is effect of relaxing bowel.
Rhizoma Gastrodiae, orchid rhizoma Gastrodiae Gastrodia elata Bl. stem tuber.It is mild-natured, it is sweet.Return liver warp.Suppressing hyperactive liver for calming endogenous wind
Only convulsion.Belong to the Inflammation Zhijing medicine of medicine for calming liver and calming endopathic wind subordinate classification.This product is mainly containing Gastrodine, Gastrodin, gastrodioside, rhizoma Gastrodiae core
The trace element such as the gloomy glycosides A of glycosides, daucosterol, Bali, adenosine, Alkaloid, polysaccharide etc., another nickeliferous, chromium, barium, manganese, zinc, copper.
Animal experiment proves that extract and decocting liquid have calm, analgesia, anticonvulsant action；Gastrodia elata polysaccharide has Enhancement test animal machine
Body nospecific immunity and cellular immunity and antiinflammatory action.It is another to have anti-aging, suppress platelet aggregation, protection cardiac muscle cell etc.
Effect.
Compared with prior art, the beneficial effects of the present invention are：
In the fertilizing method of the present invention, pass through the traditional Chinese medicine powder made of dayflower, brassica campestris L. seed and rhizoma Gastrodiae and farm manure, calcium and magnesium
Phosphate fertilizer composite fertilizer as rich water disk fertilizer, in conjunction with made of radix bupleuri, asarum, Jehol Ligusticum Rhizome and dandelion herb liquid spray the page,
So that Bu Funa tender leaf anticancer function significantly improves.
Embodiment
The invention will be further described with reference to embodiments, but the invention is not limited in these embodiments.
Embodiment 1
Improve the fertilizing method of Bu Funa tender leaf anticancer functions, including following preparation process：After Bu Funa insemination and emergences, often
Herb liquid was sprayed every 5 days, each fountain height is 10ml, continuous to spray 6 times, afterwards, starts out 20cm around away from root 30cm
Deep annular groove, applies overburden soil after rich water disk fertilizer, and it is 5kg that every plant of Bu Funa, which needs rich water disk fertilizer, the fertilising in other stages
Conventionally applied fertilizer.
Above-mentioned rich water disk fertilizer, is made up of the raw material of following parts by weight：120 parts of farm manure, 10 parts of calcium and magnesium phosphorus composite fertilizer,
25 parts of traditional Chinese medicine powder；Described farm manure is weight than 1:1 is chicken manure and duck excrement, stacks fermentation 10 days, is dried to obtain.
Above-mentioned traditional Chinese medicine powder, it is made up of the raw material of following parts by weight：46 parts of dayflower, 12 parts of brassica campestris L. seed, 18 parts of rhizoma Gastrodiae.
Above-mentioned herb liquid, it is made up of the raw material of following parts by weight：20 parts of radix bupleuri, 35 parts of asarum, 10 parts of Jehol Ligusticum Rhizome, dandelion
10 parts.
Above-mentioned Chinese medicine powder, preparation method thereof is：Traditional Chinese medicine powder whole raw material is well mixed, added same with traditional Chinese medicine powder whole raw material
Etc. the water of weight, its dusty yeast of weight 0.2% is added, ferment 20h at 30 DEG C, drying, is crushed to 250 mesh.
The preparation method of above-mentioned herb liquid is：Herb liquid whole raw material is well mixed, is crushed to 100 mesh, is added all
The water of herb liquid raw material equivalent weight, 10min, rotating speed 7000r/min are handled with high-speed shearing emulsion machine, it is then micro- with high pressure
Jet Ultra-Micro Grinding Equipment handles 8min under 48MPa pressure and at a temperature of 25 DEG C, number of processes is 2 times, obtains paste serous material；
The dusty yeast of paste serous material weight 0.3% is added, is mixed evenly, at 32 DEG C, 30h is sealed by fermentation, filtering, is filtered
Liquid, the water dilution of 30 times of filtered fluid volume is added, obtains herb liquid.
Embodiment 2
Improve the fertilizing method of Bu Funa tender leaf anticancer functions, including following preparation process：After Bu Funa insemination and emergences, often
Herb liquid was sprayed every 5 days, each fountain height is 20ml, continuous to spray 5 times, afterwards, starts out 15cm around away from root 40cm
Deep annular groove, applies overburden soil after rich water disk fertilizer, and it is 6kg that every plant of Bu Funa, which needs rich water disk fertilizer, the fertilising in other stages
Conventionally applied fertilizer.
Above-mentioned rich water disk fertilizer, is made up of the raw material of following parts by weight：100 parts of farm manure, 15 parts of calcium and magnesium phosphorus composite fertilizer,
20 parts of traditional Chinese medicine powder；Described farm manure is pig manure, stacks fermentation 13 days, is dried to obtain.
Above-mentioned traditional Chinese medicine powder, it is made up of the raw material of following parts by weight：37 parts of dayflower, 20 parts of brassica campestris L. seed, 12 parts of rhizoma Gastrodiae.
Above-mentioned herb liquid, it is made up of the raw material of following parts by weight：35 parts of radix bupleuri, 20 parts of asarum, 18 parts of Jehol Ligusticum Rhizome, dandelion
5 parts.
Above-mentioned Chinese medicine powder, preparation method thereof is：Traditional Chinese medicine powder whole raw material is well mixed, added same with traditional Chinese medicine powder whole raw material
Etc. the water of weight, its dusty yeast of weight 0.4% is added, ferment 26h at 33 DEG C, drying, is crushed to 200 mesh.
The preparation method of above-mentioned herb liquid is：Herb liquid whole raw material is well mixed, is crushed to 150 mesh, is added all
The water of herb liquid raw material equivalent weight, 9min, rotating speed 8000r/min are handled with high-speed shearing emulsion machine, it is then micro- with high pressure
Jet Ultra-Micro Grinding Equipment handles 11min under 55MPa pressure and at a temperature of 25 DEG C, number of processes is 3 times, obtains paste slurry
Liquid；The dusty yeast of paste serous material weight 0.5% is added, is mixed evenly, at 30 DEG C, 36h is sealed by fermentation, filtering, obtains
Filtered fluid, the water dilution of 25 times of filtered fluid volume is added, obtains herb liquid.
Embodiment 3
Improve the fertilizing method of Bu Funa tender leaf anticancer functions, including following preparation process：After Bu Funa insemination and emergences, often
Herb liquid was sprayed every 5 days, each fountain height is 30ml, continuous to spray 4 times, afterwards, starts out 10cm around away from root 50cm
Deep annular groove, applies overburden soil after rich water disk fertilizer, and it is 7kg that every plant of Bu Funa, which needs rich water disk fertilizer, the fertilising in other stages
Conventionally applied fertilizer.
Above-mentioned rich water disk fertilizer, is made up of the raw material of following parts by weight：80 parts of farm manure, 20 parts of calcium and magnesium phosphorus composite fertilizer,
15 parts of traditional Chinese medicine powder；Described farm manure is chicken manure, stacks fermentation 15 days, is dried to obtain.
Above-mentioned traditional Chinese medicine powder, it is made up of the raw material of following parts by weight：28 parts of dayflower, 28 parts of brassica campestris L. seed, 5 parts of rhizoma Gastrodiae.
Above-mentioned herb liquid, it is made up of the raw material of following parts by weight：50 parts of radix bupleuri, 5 parts of asarum, 25 parts of Jehol Ligusticum Rhizome, dandelion 1
Part.
Above-mentioned Chinese medicine powder, preparation method thereof is：Traditional Chinese medicine powder whole raw material is well mixed, added same with traditional Chinese medicine powder whole raw material
Etc. the water of weight, its dusty yeast of weight 0.6% is added, ferment 32h at 35 DEG C, drying, is crushed to 150 mesh.
The preparation method of above-mentioned herb liquid is：Herb liquid whole raw material is well mixed, is crushed to 200 mesh, is added all
The water of herb liquid raw material equivalent weight, 8min, rotating speed 9000r/min are handled with high-speed shearing emulsion machine, it is then micro- with high pressure
Jet Ultra-Micro Grinding Equipment handles 14min under 48MPa pressure and at a temperature of 25 DEG C, number of processes is 2 times, obtains paste slurry
Liquid；The dusty yeast of paste serous material weight 0.8% is added, is mixed evenly, at 28 DEG C, 42h is sealed by fermentation, filtering, obtains
Filtered fluid, the water dilution of 20 times of filtered fluid volume is added, obtains herb liquid.
Comparative example 1
Improve the fertilizing method of Bu Funa tender leaf anticancer functions, including following preparation process：After Bu Funa insemination and emergences, often
Herb liquid was sprayed every 5 days, each fountain height is 20ml, continuous to spray 5 times, afterwards, starts out 15cm around away from root 40cm
Deep annular groove, applies overburden soil after rich water disk fertilizer, and it is 6kg that every plant of Bu Funa, which needs rich water disk fertilizer, the fertilising in other stages
Conventionally applied fertilizer.
Above-mentioned rich water disk fertilizer, is made up of the raw material of following parts by weight：100 parts of farm manure, 15 parts of calcium and magnesium phosphorus composite fertilizer；
Described farm manure is pig manure, stacks fermentation 13 days, is dried to obtain.
Above-mentioned herb liquid, it is made up of the raw material of following parts by weight：35 parts of radix bupleuri, 20 parts of asarum, 18 parts of Jehol Ligusticum Rhizome, dandelion
5 parts.
The preparation method of above-mentioned herb liquid is：Herb liquid whole raw material is well mixed, is crushed to 150 mesh, is added all
The water of herb liquid raw material equivalent weight, 9min, rotating speed 8000r/min are handled with high-speed shearing emulsion machine, it is then micro- with high pressure
Jet Ultra-Micro Grinding Equipment handles 11min under 55MPa pressure and at a temperature of 25 DEG C, number of processes is 3 times, obtains paste slurry
Liquid；The dusty yeast of paste serous material weight 0.5% is added, is mixed evenly, at 30 DEG C, 36h is sealed by fermentation, filtering, obtains
Filtered fluid, the water dilution of 25 times of filtered fluid volume is added, obtains herb liquid.
Comparative example 2
Improve the fertilizing method of Bu Funa tender leaf anticancer functions, including following preparation process：After Bu Funa insemination and emergences, 25
After it, start out the deep annular grooves of 15cm around away from root 40cm, apply overburden soil after rich water disk fertilizer, every plant of Bu Funa
It is 6kg to need rich water disk fertilizer, and the fertilising in other stages is conventionally applied fertilizer.
Above-mentioned rich water disk fertilizer, is made up of the raw material of following parts by weight：100 parts of farm manure, 15 parts of calcium and magnesium phosphorus composite fertilizer,
20 parts of traditional Chinese medicine powder；Described farm manure is pig manure, stacks fermentation 13 days, is dried to obtain.
Above-mentioned traditional Chinese medicine powder, it is made up of the raw material of following parts by weight：37 parts of dayflower, 20 parts of brassica campestris L. seed, 12 parts of rhizoma Gastrodiae.
Above-mentioned Chinese medicine powder, preparation method thereof is：Traditional Chinese medicine powder whole raw material is well mixed, added same with traditional Chinese medicine powder whole raw material
Etc. the water of weight, its dusty yeast of weight 0.4% is added, ferment 26h at 33 DEG C, drying, is crushed to 200 mesh.
Test example：The tender leaf antineoplastic action effect that fertilizing method of the present invention is prepared
L materials and methods
1.1 sample
The tender leaf for cultivating to obtain according to the embodiment of the present invention 2, comparative example 1 and the fertilizing method of comparative example 2.
Human body recommended intake be ll.7mg/kg.bw/d (0.7~l.4g/d, in terms of 0.7g/d；Human body weight with
60kg is counted).
1.2 tumor-bearing mice
There is provided by certain institute of materia medica.
1.3 experimental animal
Male ICR mouse 40,20~23g of body weight, is provided by certain Experimental Animal Center.
Animal is randomly divided into Normal group (giving 0.5% starch fluid), the embodiment of the present invention 2, comparative example 1 and comparative example
The tender leaf that 2 fertilizing methods cultivate to obtain each 58.5mg/kg.bw/d (equivalent to human body recommended intake ll.7mg/kg.bw/d
5 times).The tender leaf that the embodiment of the present invention 2, comparative example 1 and the fertilizing method of comparative example 2 cultivate to obtain (is boiled with 0.5% starch fluid
Boiling, after cooling) it is configured to suspension.All experimental animals give edible full-valence pellet feed, drinking-water of freely ingesting.
1.4 test method
The gavage volume number that each group animal gives tested material is 0.02mL/g.bw, l times/d, when gavage is to 20d, per mouse
The subcutaneous aseptic inoculation S-l80 sarcoma cells suspension of right fore [25% (volume fraction), with normal saline dilution] 0.2mL.Inoculation
Continue to stop gavage after gavage l0d afterwards, after 3d, cervical dislocation puts to death mouse, and tumour, tumor surface blood stains filter paper are peeled off per mouse
Weighed after blotting.
1.5 data statistics
The data obtained each experimental group is compared.
2 result of the tests
2.1 body weight
Each group mouse weight situation of change is shown in Table l.As a result the embodiment of the present invention 2, comparative example 1 and the fertilizing method of comparative example 2
Obtained tender leaf each group mouse weight is cultivated compared with Normal group, no significant difference (P>0.05).
The tender leaf that 1 fertilizing method of the present invention of table is prepared suppresses Tumor Assays mouse weight situation
| Group | Number of animals (only) | Starting weight (g) | 20d body weight (g) | 33d body weight (g) |
| Normal group | 10 | 20.9±0.3 | 32.5±1.2 | 33.4±1.2 |
| 2 groups of embodiment | 10 | 21.2±0.5 | 33.1±1.5 | 32.1±1.5 |
| 1 group of comparative example | 10 | 22.3±0.4 | 32.8±2.1 | 30.5±1.1 |
| 2 groups of comparative example | 10 | 20.6±0.6 | 31.1±1.8 | 31.2±1.8 |
Note：Body weight is to go body weight after knurl during 33d.
2.2 suppress the experiment of S-180 sarcomas
Suppress S-l80 sarcomas experiment each group mouse knurl weight and the results are shown in Table 2.Experimental data therein, it is mean value ± standard
Difference.
Influence of the tender leaf that 2 fertilizing method of the present invention of table is prepared to small s-180 sarcoma weight
The result of table 2 shows that the tender leaf of embodiment 2, comparative example 1 and comparative example 2, which all has, suppresses mouse S-l80 growing sarcomas
Effect, but the inhibition of embodiment 2 is notable compared with comparative example 1 and comparative example 2, illustrate to add traditional Chinese medicine powder of the present invention and herb liquid
Bu Funa tender leaf anticancer functions can be significantly improved.
Claims (9)
1. improve the fertilizing method of Bu Funa tender leaf anticancer functions, it is characterised in that：Including following preparation process：Bu Funa is sowed
After emergence, herb liquid was sprayed every 5 days, each fountain height is 10-30ml, continuous to spray 4-6 times, afterwards, away from root 30-
Start out the deep annular grooves of 10-20cm around 50cm, apply overburden soil after rich water disk fertilizer, the fertilising in other stages is according to routine
Method is applied fertilizer；
Described rich water disk fertilizer, is made up of the raw material of following parts by weight：Farm manure 80-120 parts, calcium and magnesium phosphorus composite fertilizer 10-20
Part, traditional Chinese medicine powder 15-25 parts；
Described traditional Chinese medicine powder, it is made up of the raw material of following parts by weight：Dayflower 28-46 parts, brassica campestris L. seed 12-28 parts, rhizoma Gastrodiae 5-18
Part；
Described herb liquid, it is made up of the raw material of following parts by weight：Radix bupleuri 20-50 parts, asarum 5-35 parts, Jehol Ligusticum Rhizome 10-25 parts, Pu
Public English 1-10 parts.
2. the fertilizing method of raising Bu Funa tender leaf anticancer functions according to claim 1, it is characterised in that：Described fertilizer
Water pond fertilizer, it is made up of the raw material of following parts by weight：100 parts of farm manure, 15 parts of calcium and magnesium phosphorus composite fertilizer, 20 parts of traditional Chinese medicine powder.
3. the fertilizing method of raising Bu Funa tender leaf anticancer functions according to claim 1, it is characterised in that：In described
Medicinal powder, it is made up of the raw material of following parts by weight：37 parts of dayflower, 20 parts of brassica campestris L. seed, 12 parts of rhizoma Gastrodiae.
4. the fertilizing method of raising Bu Funa tender leaf anticancer functions according to claim 1, it is characterised in that：In described
Decoction, it is made up of the raw material of following parts by weight：35 parts of radix bupleuri, 20 parts of asarum, 18 parts of Jehol Ligusticum Rhizome, 5 parts of dandelion.
5. the fertilizing method of raising Bu Funa tender leaf anticancer functions according to claim 1, it is characterised in that：Described agriculture
Family's fertilizer is animal wastes, stacks fermentation 10-15 days, is dried to obtain.
6. the fertilizing method of raising Bu Funa tender leaf anticancer functions according to claim 5, it is characterised in that：Described is dynamic
Thing excrement is the one or more in chicken manure, duck excrement, pig manure.
7. the fertilizing method of the raising Bu Funa tender leaf anticancer functions according to claim 1 or 3, it is characterised in that：It is described
Chinese medicine powder, preparation method thereof is：Traditional Chinese medicine powder whole raw material is well mixed, adds the water with traditional Chinese medicine powder whole raw material equivalent weight, then
Its weight 0.2-0.6% dusty yeasts are added, ferment 20-32h at 30-35 DEG C, drying, is crushed to 150-250 mesh.
8. the fertilizing method of the raising Bu Funa tender leaf anticancer functions according to claim 1 or 4, it is characterised in that：It is described
The preparation method of herb liquid is：Herb liquid whole raw material is well mixed, is crushed to 100-200 mesh, it is former to add whole herb liquids
Expect the water of equivalent weight, 8-10min, rotating speed 7000-9000r/min are handled with high-speed shearing emulsion machine, it is then micro- with high pressure
Jet Ultra-Micro Grinding Equipment handles 8-14min under 48-62MPa pressure and at a temperature of 25 DEG C, number of processes is 2-3 times, is obtained
Paste serous material；Paste serous material weight 0.3-0.8% dusty yeast is added, is mixed evenly, at 28-32 DEG C, is sealed by fermentation
30-42h, filtering, filtered fluid is obtained, add the water dilution of 20-30 times of filtered fluid volume, obtain herb liquid.
9. the fertilizing method of raising Bu Funa tender leaf anticancer functions according to claim 1, it is characterised in that：Every plant of cloth good fortune
It is 5-7kg that Na, which needs rich water disk fertilizer,.
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