CN107699524A - A kind of preparation method of active black saccharolactic acid bacterium - Google Patents
A kind of preparation method of active black saccharolactic acid bacterium Download PDFInfo
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- CN107699524A CN107699524A CN201711091181.3A CN201711091181A CN107699524A CN 107699524 A CN107699524 A CN 107699524A CN 201711091181 A CN201711091181 A CN 201711091181A CN 107699524 A CN107699524 A CN 107699524A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a kind of preparation method of active black saccharolactic acid bacterium, first in existing probiotics population screening can on the culture medium that brown sugar is prepared fast-growth, the strain of breeding, continuously optimize fermentation condition.One plant of energy fast-growth, lactic bacteria strain JSX BS2 of breeding on brown sugar culture medium have therefrom been screened, afterwards, depth fermentation has been carried out to this plant of JSX BS2 with brown sugar culture medium.Then embedding protective agent is added to tunning, deep-freezing, which is dried, is processed into probiotic powder, and then produces the lactic acid bacteria health-oriented products of different dosage forms.Product contain brown sugar nutritional ingredient, lactic acid bacteria and its metabolite, it is more abundanter than the nutrition of original lactic acid bacteria product, and can prevention of various diseases, body immunity is improved, beneficial to health, suitable for the crowd of each age level, especially women.
Description
Technical field
The present invention relates to a kind of preparation method of active black saccharolactic acid bacterium, and in particular to the fermentation of probiotics and healthy food
Prepare, belong to food fermentation field of engineering technology.
Background technology
Probiotics is that one kind can improve host's microecological balance, produce definite health efficacy so as to play the work of beneficial effect
The general name of property microorganism.Probiotics is generally divided into three major types, including：Lactobacillus class（Such as lactobacillus acidophilus, Lactobacillus casei
Deng）；Bifidobacterium class（Such as bifidobacterium longum, bifidobacterium breve, bifidobacterium thermophilum）；And gram-positive cocci（Such as
Streptococcus fecalis, galactococcus etc.）.In addition, there is the category that some saccharomycete can also be included into probiotics.Probiotics mainly has to human body
Effect and function below：Breeding of the pernicious bacteria in enteron aisle can be suppressed, toxin is reduced and produce, promote intestines peristalsis, so as to carry
High enteron aisle function, improving defecation condition, promote digestive system health, help absorbs nutritional ingredient, reduces serum cholesterol, and
Strengthen body immunity, contribute to antitumor, suppression cancer；Probiotics can also prevent or improve diarrhoea, alleviate lactase deficiency shape, carry
High anaphylaxis tolerance, prevention genital system infection etc..Probiotics is in addition to the action and function of itself, also in growth and breeding mistake
Cheng Dangzhong is produced, secretion largely beneficial to the metabolite of health, such as generates a large amount of vitamins, antioxidant, digestive ferment will
Lactic acid and acetic acid are generated after sugar decomposition, enteron aisle is bred in acidity, suppression pernicious bacteria, reduces toxin and produce, stimulate enterocinesia,
And the utilization rate of calcium, phosphorus, iron can be improved, promote the absorption of iron and vitamin D；Lactase is produced, helps people digest's lactose etc..
A variety of floras in human gastrointestinal tract digestive system be present, it is beneficial（Probiotics）, it is harmful and neutral, in normal condition
A kind of natural equilibrium be present in these lower floras.
But modern diet, live, under working voltage power and pathological conditions, the quantity rise of pernicious bacteria, neutral bacterium
Group produces harmful material, becomes pernicious bacteria, so as to cause human colon and digestive system flora natural equilibrium to lose
Balance：Aerogenesis, abdominal distension, diarrhoea and constipation etc., are easily infected by pathogen, and human body is in inferior health or pathological state.Supplement benefit
Raw bacterium, which has, to be promoted in recovery human body natural's balance：Gastrointestinal bacterial flora balance is maintained, stomach and intestine normal function is kept, promotes gastro-intestinal digestion system
System health and raising human immunological competence etc..So probiotics is increasingly subject to the attention of people, probiotics industry is thus brought
Fast development.At present, the probiotics raw material production of probiotic products is mainly by the microbiological culture media culture of routine, probiotics
Growth need to provide a large amount of carbon sources and nitrogen source, generally, during large-scale production, white granulated sugar is used as carbon source.
Brown sugar is a kind of sucrose without by the honey shaping of highly refined band, and also known as red sugar, purple sugar, color is deep,
With burnt odor taste, the redness of the skin or complexion crystalline solid formed is refined through pressure extracting juice for the stem of gramineous herbaceous plant sugarcane, containing abundant
Sugared part, mineral matter and Glycolic acid.Contain substantial amounts of nutriment in brown sugar has original work(to the health of skin, nutrition
Effect, can accelerate the metabolism of Skin Cell, supplement the nutrients, and promote to regenerate, natural acids and the pigment instrumentality containing uniqueness in brown sugar
Matter, can effectively adjust various metabolism of pigment processes, colouring matter secretion quantity and Magnifying chromoscopy in balance skin, reduce local pigment
Abnormal stacking.Body warmer can be allowed by drinking the brown sugar water of heat, increased energy, active qi and blood, accelerated blood circulation, have and promote in woman month
Through smooth.
It is contemplated that replacing carbon source of the white granulated sugar as growth of probiotics to cultivate probiotics with brown sugar, produce and be rich in
The probiotics of brown sugar nutritional ingredient, the product of probiotics and its processing is more rich in nutrition and conditioning functions, be suitable for all kinds of
Crowd, especially women.
The content of the invention
Purpose：To solve the deficiencies in the prior art, the invention provides a kind of preparation method of active black saccharolactic acid bacterium.
Technical scheme：In order to solve the above technical problems, the technical solution adopted by the present invention is：
A kind of preparation method of active black saccharolactic acid bacterium, its step are：
(1) MRS solid mediums, JSX-BS2 strains are activated under the conditions of 37 DEG C；
(2) the JSX-BS2 strains of inoculation activation are in the MRS fluid nutrient mediums of improvement；
(3) 200rpm, ferment 1-2 days under the conditions of 37 DEG C；
(4) sampling detection；
(5) zymotic fluid pH value 4.0-4.5, lactic acid bacteria number reach 1010Fermentation can be terminated during individual/ml, obtains zymotic fluid；
(6) sterilized milk powder is added in zymotic fluid, mixed, then deep-freezing is dried to streptococcus acidi lactici fermented solution freeze-dried powder；
(7) a certain amount of additive is added, is mixed with streptococcus acidi lactici fermented solution freeze-dried powder, is processed into dosage forms, produces brown sugar breast
Sour bacterium finished product.
The preparation method of described active black saccharolactic acid bacterium, it is characterised in that：The configuration of MRS solid mediums：10g/L eggs
White peptone, 10g/L beef extracts, 5g/L dusty yeasts, 20g/L glucose, 5g/L anhydrous sodium acetates, 1ml/L Tween-80s, 2g/L lemons
Acid diamine, 2g/L dipotassium hydrogen phosphates, 0.58g/L magnesium sulfate, 0.25g/L manganese sulfates, are dissolved with distilled water, regulation pH 6.6-
6.8, then add 2% agar, constant volume；High pressure 1 × 105Pa sterilizings 20min.
The preparation method of described active black saccharolactic acid bacterium, it is characterised in that：The configuration of the MRS fluid nutrient mediums of improvement：
10g/L peptones, 10g/L beef extracts, 5g/L dusty yeasts, 10-30g/L brown sugar, 5g/L anhydrous sodium acetates, 1ml/L Tween-80s,
2g/L lemons acid diamine, 2g/L dipotassium hydrogen phosphates, 0.58g/L magnesium sulfate, 0.25g/L manganese sulfates, are dissolved with distilled water, adjust pH
6.6-6.8 and constant volume；High pressure 1 × 105Pa sterilizings 20min.
The preparation method of described active black saccharolactic acid bacterium, it is characterised in that：Sampling detection includes：Zymotic fluid pH value and mirror
Inspection and lactic acid bacterial count.
Preferably, the preparation method of described active black saccharolactic acid bacterium, it is characterised in that：Step（6）Middle milk powder
Addition be zymotic fluid quality 5-10%.
Preferably, the preparation method of described active black saccharolactic acid bacterium, it is characterised in that：The additive is can
One or more in feeding habits starch, cycloheptaamylose, magnesium stearate.
Beneficial effect：The preparation method of active black saccharolactic acid bacterium provided by the invention, first from existing probiotics population
In screening can brown sugar prepare culture medium on fast-growth, breeding strain, continuously optimize fermentation condition.Therefrom screen
One plant on brown sugar culture medium can fast-growth, lactic bacteria strain --- the JSX-BS2 of breeding, afterwards, with brown sugar culture medium to this
Strain JSX-BS2 carries out depth fermentation.Then embedding protective agent being added to tunning, deep-freezing, which is dried, is processed into probiotic powder,
And then produce the lactic acid bacteria health-oriented products of different dosage forms.Product contain brown sugar nutritional ingredient, lactic acid bacteria and its metabolite,
It is more abundanter than the nutrition of original lactic acid bacteria product, and energy prevention of various diseases, raising body immunity, beneficial health,
Suitable for the crowd of each age level, especially women.
Embodiment
The present invention is illustrated with reference to example：
Embodiment 1：
A kind of preparation method of active black saccharolactic acid bacterium, its step are：
（1）The configuration of MRS solid mediums：10g/L peptones, 10g/L beef extracts, 5g/L dusty yeasts, 20g/L glucose, 5g/
L anhydrous sodium acetates, 1ml/L Tween-80s, 2g/L lemons acid diamine, 2g/L dipotassium hydrogen phosphates, 0.58g/L magnesium sulfate, 0.25g/L
Manganese sulfate, dissolved with distilled water, adjust pH 6.6-6.8, then add 2% agar, constant volume；High pressure 1 × 105Pa sterilizings 20min；
（2）The configuration of the MRS fluid nutrient mediums of improvement：10g/L peptones, 10g/L beef extracts, 5g/L dusty yeasts, 10g/L are black
Sugar, 5g/L anhydrous sodium acetates, 1ml/L Tween-80s, 2g/L lemons acid diamine, 2g/L dipotassium hydrogen phosphates, 0.58g/L magnesium sulfate,
0.25g/L manganese sulfates, are dissolved with distilled water, adjust pH 6.6-6.8, and constant volume；High pressure 1 × 105Pa sterilizings 20min；
（3）With activation JSX-BS2 strains under the conditions of MRS solid mediums, 37 DEG C；
（4）Inoculation activation JSX-BS2 strains are in the MRS fluid nutrient mediums of improvement；
（5）200rpm, ferment about 1-2 days under the conditions of 37 DEG C；
（6）Sampling detection, including：Zymotic fluid pH value and microscopy and lactic acid bacterial count；
（7）Zymotic fluid pH value 4.0-4.5, lactic acid bacteria number reach>1010Fermentation can be terminated during/ml；
（8）5-10% sterilized milk powder is added in zymotic fluid, mixed, then deep-freezing is dried to streptococcus acidi lactici fermented solution
Freeze-dried powder；
（9）A certain amount of sterilized filler such as edible starch, cycloheptaamylose and magnesium stearate etc. are added, with（8）Middle breast
Acid bacteria fermentation liquid freeze-dried powder mixes, and is processed into different dosage forms, as brown sugar lactobacillus completed product.
Embodiment 2：
（1）The configuration of MRS solid mediums：10g/L peptones, 10g/L beef extracts, 5g/L dusty yeasts, 20g/L glucose, 5g/
L anhydrous sodium acetates, 1ml/L Tween-80s, 2g/L lemons acid diamine, 2g/L dipotassium hydrogen phosphates, 0.58g/L magnesium sulfate, 0.25g/L
Manganese sulfate, dissolved with distilled water, adjust pH 6.6-6.8, then add 2% agar, constant volume；High pressure 1 × 105Pa sterilizings 20min；
（2）The configuration of the MRS fluid nutrient mediums of improvement：10g/L peptones, 10g/L beef extracts, 5g/L dusty yeasts, 20g/L are black
Sugar, 5g/L anhydrous sodium acetates, 1ml/L Tween-80s, 2g/L lemons acid diamine, 2g/L dipotassium hydrogen phosphates, 0.58g/L magnesium sulfate,
0.25g/L manganese sulfates, are dissolved with distilled water, adjust pH 6.6-6.8, and constant volume；High pressure 1 × 105Pa sterilizings 20min；
（3）With activation JSX-BS2 strains under the conditions of MRS solid mediums, 37 DEG C；
（4）The JSX-BS2 strains of activation are inoculated with the MRS fluid nutrient mediums of improvement；
（5）200rpm, ferment about 1-2 days under the conditions of 37 DEG C；
（6）Sampling detection, including：Zymotic fluid pH value and microscopy and lactic acid bacterial count；
（7）Zymotic fluid pH value 4.0-4.5, lactic acid bacteria number reach>1010Fermentation can be terminated during/ml；
（8）5-10% sterilized milk powder is added in zymotic fluid, mixed, then deep-freezing is dried to streptococcus acidi lactici fermented solution
Freeze-dried powder；
（9）A certain amount of sterilized filler such as edible starch, cycloheptaamylose and magnesium stearate etc. are added, with（8）Middle breast
Acid bacteria fermentation liquid freeze-dried powder mixes, and is processed into different dosage forms, as brown sugar lactobacillus completed product.
Embodiment 3：
（1）The configuration of MRS solid mediums：10g/L peptones, 10g/L beef extracts, 5g/L dusty yeasts, 20g/L glucose, 5g/
L anhydrous sodium acetates, 1ml/L Tween-80s, 2g/L lemons acid diamine, 2g/L dipotassium hydrogen phosphates, 0.58g/L magnesium sulfate, 0.25g/L
Manganese sulfate, dissolved with distilled water, adjust pH 6.6-6.8, then add 2% agar, constant volume；High pressure 1 × 105Pa sterilizings 20min；
（2）The configuration of the MRS fluid nutrient mediums of improvement：10g/L peptones, 10g/L beef extracts, 5g/L dusty yeasts, 30g/L are black
Sugar, 5g/L anhydrous sodium acetates, 1ml/L Tween-80s, 2g/L lemons acid diamine, 2g/L dipotassium hydrogen phosphates, 0.58g/L magnesium sulfate,
0.25g/L manganese sulfates, are dissolved with distilled water, adjust pH 6.6-6.8, and constant volume；High pressure 1 × 105Pa sterilizings 20min；
（3）With activation JSX-BS2 strains under the conditions of MRS solid mediums, 37 DEG C；
（4）The JSX-BS2 strains of activation are inoculated with the MRS fluid nutrient mediums of improvement；
（5）200rpm, ferment about 1-2 days under the conditions of 37 DEG C；
（6）Sampling detection, including：Zymotic fluid pH value and microscopy and lactic acid bacterial count；
（7）Zymotic fluid pH value 4.0-4.5, lactic acid bacteria number reach>1010Fermentation can be terminated during/ml；
（8）5-10% sterilized milk powder is added in zymotic fluid, mixed, then deep-freezing is dried to streptococcus acidi lactici fermented solution
Freeze-dried powder；
（9）A certain amount of sterilized filler such as edible starch, cycloheptaamylose and magnesium stearate etc. are added, with（8）Middle breast
Acid bacteria fermentation liquid freeze-dried powder mixes, and is processed into different dosage forms, as brown sugar lactobacillus completed product.
The present invention solves the problems, such as the fermentation condition optimization of brown sugar culture probiotics, including：Make medium component with brown sugar
Excellent species screening, the most suitable probiotics fermention concentration of brown sugar determines.
It is contemplated that replacing carbon source of the white granulated sugar as growth of probiotics to cultivate probiotics with brown sugar, produce and be rich in
The probiotics of brown sugar nutritional ingredient, the product of probiotics and its processing is more rich in nutrition and conditioning functions, be suitable for all kinds of
Crowd, especially women.
The present invention is disclosed with preferred embodiment above, so it is not intended to limiting the invention, all to use equivalent substitution
Or the technical scheme that equivalent transformation mode is obtained, it is within the scope of the present invention.
Claims (6)
1. a kind of preparation method of active black saccharolactic acid bacterium, its step are：
(1) MRS solid mediums, JSX-BS2 strains are activated under the conditions of 37 DEG C；
(2) the JSX-BS2 strains of inoculation activation are in the MRS fluid nutrient mediums of improvement；
(3) 200rpm, ferment 1-2 days under the conditions of 37 DEG C；
(4) sampling detection；
(5) zymotic fluid pH value 4.0-4.5, lactic acid bacteria number reach 1010Fermentation can be terminated during individual/ml, obtains zymotic fluid；
(6) sterilized milk powder is added in zymotic fluid, mixed, then deep-freezing is dried to streptococcus acidi lactici fermented solution freeze-dried powder；
(7) a certain amount of additive is added, is mixed with streptococcus acidi lactici fermented solution freeze-dried powder, is processed into dosage forms, produces brown sugar breast
Sour bacterium finished product.
2. the preparation method of active black saccharolactic acid bacterium according to claim 1, it is characterised in that：MRS solid mediums
Configuration：10g/L peptones, 10g/L beef extracts, 5g/L dusty yeasts, 20g/L glucose, 5g/L anhydrous sodium acetates, 1ml/L tweens-
80th, 2g/L lemons acid diamine, 2g/L dipotassium hydrogen phosphates, 0.58g/L magnesium sulfate, 0.25g/L manganese sulfates, are dissolved with distilled water, are adjusted
PH 6.6-6.8 are saved, then add 2% agar, constant volume；High pressure 1 × 105Pa sterilizings 20min.
3. the preparation method of active black saccharolactic acid bacterium according to claim 1, it is characterised in that：The MRS liquid training of improvement
Support the configuration of base：10g/L peptones, 10g/L beef extracts, 5g/L dusty yeasts, 10-30g/L brown sugar, 5g/L anhydrous sodium acetates,
1ml/L Tween-80s, 2g/L lemons acid diamine, 2g/L dipotassium hydrogen phosphates, 0.58g/L magnesium sulfate, 0.25g/L manganese sulfates, with distillation
Water dissolves, and adjusts pH 6.6-6.8, and constant volume；High pressure 1 × 105Pa sterilizings 20min.
4. the preparation method of active black saccharolactic acid bacterium according to claim 1, it is characterised in that：Sampling detection includes：Hair
Zymotic fluid pH value and microscopy and lactic acid bacterial count.
5. the preparation method of active black saccharolactic acid bacterium according to claim 1, it is characterised in that：Step（6）Middle milk powder
Addition is the 5-10% of zymotic fluid quality.
6. the preparation method of active black saccharolactic acid bacterium according to claim 1, it is characterised in that：The additive is edible
One or more in property starch, cycloheptaamylose, magnesium stearate.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107927744A (en) * | 2017-11-08 | 2018-04-20 | 苏州健世星生物科技有限公司 | A kind of preparation method of high nutrition radix bardanae healthy food |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006007463A1 (en) * | 2004-07-01 | 2006-01-19 | General Mills, Inc. | Cultures encapsulated with compound fat breakfast cereals coated with compound fat and methods of preparation |
| WO2011115114A1 (en) * | 2010-03-19 | 2011-09-22 | 株式会社ヤクルト本社 | Novel lactobacillus classified as lactobacillus plantarum, and use thereof |
| CN103221531A (en) * | 2010-11-09 | 2013-07-24 | 可尔必思株式会社 | Bacterium lactobacillus helveticus having high proteolysis activity |
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2017
- 2017-11-08 CN CN201711091181.3A patent/CN107699524A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006007463A1 (en) * | 2004-07-01 | 2006-01-19 | General Mills, Inc. | Cultures encapsulated with compound fat breakfast cereals coated with compound fat and methods of preparation |
| WO2011115114A1 (en) * | 2010-03-19 | 2011-09-22 | 株式会社ヤクルト本社 | Novel lactobacillus classified as lactobacillus plantarum, and use thereof |
| CN103221531A (en) * | 2010-11-09 | 2013-07-24 | 可尔必思株式会社 | Bacterium lactobacillus helveticus having high proteolysis activity |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107927744A (en) * | 2017-11-08 | 2018-04-20 | 苏州健世星生物科技有限公司 | A kind of preparation method of high nutrition radix bardanae healthy food |
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