CN102008110B - Multi-strain microbial composite beverage preparation and preparation method thereof - Google Patents
Multi-strain microbial composite beverage preparation and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 65
- 235000013361 beverage Nutrition 0.000 title claims abstract description 39
- 230000000813 microbial effect Effects 0.000 title claims abstract description 33
- 239000002131 composite material Substances 0.000 title claims abstract description 32
- 238000011218 seed culture Methods 0.000 claims abstract description 76
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 61
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 49
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 46
- 241000894006 Bacteria Species 0.000 claims abstract description 41
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 31
- 241000194020 Streptococcus thermophilus Species 0.000 claims abstract description 31
- 230000001580 bacterial effect Effects 0.000 claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 9
- 230000004151 fermentation Effects 0.000 claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 8
- 239000012531 culture fluid Substances 0.000 claims description 75
- 239000012530 fluid Substances 0.000 claims description 36
- 235000015097 nutrients Nutrition 0.000 claims description 34
- 239000002609 medium Substances 0.000 claims description 30
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 19
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 19
- 241000194017 Streptococcus Species 0.000 claims description 19
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- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 19
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention relates to a multi-strain microbial composite beverage preparation and a preparation method thereof. The multi-strain microbial composite beverage preparation is characterized in that: seed culture solution is obtained by performing single-strain culture of bacillus natto, lactobacillus, bifidobacterium, streptococcus thermophilus and bulgarian bacillus respectively, the culture solution is inoculated into the same culture medium, and the culture solution is subjected to the culture of a fermentation tank until the pH value of the culture solution is between 3 and 3.5 to obtain the multi-strain microbial composite beverage preparation, wherein the bacterial count of each strain in the preparation is more than or equal to 5.0*10<9>cfu/ml. The multi-strain microbial composite beverage preparation can promote the proliferation of in-vivo beneficial bacteria, inhibit harmful bacteria from propagating, provide necessary materials for the growth and functional exertion of beneficial microbes of human bodies, regulate the micro-ecological balance of gastrointestinal tracts and reproduction systems of the human bodies to enable the human bodies to reach a micro-ecological balance, enhance appetites, promote digestion, and prevent acute and chronic diseases in the gastrointestinal tracts simultaneously.
Description
Technical field
The invention belongs to the food and drink field, particularly a kind of many strain microbial composite beverages preparation and preparation method thereof.
Background technology
At present, food and drink can be divided into fruit juice and beverage, vegetable juice and beverage thereof, phytoprotein beverage, plant extract beverage, milk beverage, soda, mineral water, solid beverage and Other Drinks.Beverage types is various, and market is huge, and the research of relevant pure microbial beverage report is less.
Summary of the invention
The present invention be directed to the deficiencies in the prior art, propose a kind of many strain microbial composite beverages preparation and preparation method thereof.This many strain microbial composite beverages preparation can promote the propagation of beneficial bacterium in the body, suppress the breeding of harmful bacteria, for human body beneficial microorganism growth and Function provide essential material, microecological balance in balance the body intestines and stomach and the reproductive system, make human body be in microecological balance, whet the appetite, promote digestion, prevent simultaneously the utmost point chronic disease in the various intestines and stomach.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is:
A kind of many strain microbial composite beverages preparation is characterized in that: it be with Bacillus natto (
Bacillus natto Swamura) ACCC 10614, Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092, Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855 and bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) CGMCC 1.1480 carries out respectively single bacterial classification and cultivates the seed culture fluid that obtains and be inoculated in the same culture medium, then carry out fermentation tank culture to the pH value of nutrient solution and be 3～3.5 and obtain, Bacillus natto in the described preparation (
Bacillus natto Swamura) the bacterium number 〉=5.0 * 10 of ACCC 10614
9Cfu/ml, Bacillus acidi lactici (
Lactococcus lactis) the bacterium number 〉=5.0 * 10 of ACCC 11092
9Cfu/ml, Bifidobacterium (
BifidobacteriumSp) the bacterium number of ACCC 11054 〉=5.0 * 10
9Cfu/ml, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) the bacterium number of CGMCC 1.1855 〉=5.0 * 10
9Cfu/ml, bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) the bacterium number of CGMCC 1.1480 〉=5.0 * 10
9Cfu/ml.
Press such scheme, the percetage by weight of the seed culture fluid of described each bacterial classification is: the seed culture fluid 25%～35% of Bacillus natto, the seed culture fluid 10%～15% of Bacillus acidi lactici, the seed culture fluid 15%～25% of Bifidobacterium, the seed culture fluid 15%～25% of streptococcus thermophilus, the seed culture fluid 10%～15% of bacillus bulgaricus.
A kind of preparation method of many strain microbial composite beverages preparation may further comprise the steps:
(1) preparation of seed culture fluid:
Bacillus natto (
Bacillus natto Swamura) preparation of ACCC 10614 seed culture fluids: with Bacillus natto (
Bacillus natto Swamura) ACCC 10614 is inoculated in the fluid nutrient medium under aseptic condition, described fluid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, the pH value transfers to 7.0, cultivated 48 hours, and obtained Bacillus natto bacterium number 〉=5.0 * 10 for 28 ℃～32 ℃
9The seed culture fluid of cfu/ml;
Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855, Bacillus acidi lactici (
Lactococcus lactis) preparation of seed culture fluid of ACCC 11092: respectively with Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855, Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092 is inoculated in the fluid nutrient medium under aseptic condition, the content of each component in 1.0L distilled water is in the described fluid nutrient medium: peptone 10.1g, beef extract 10.0g, dusty yeast 5.0g, glucose 5.0g, sodium acetate 5.0g, 28 ℃～32 ℃ single bacterial classifications were cultivated 24～48 hours, obtained respectively Bifidobacterium bacterium number 〉=5.0 * 10
9The seed culture fluid of cfu/ml, streptococcus thermophilus bacterium number 〉=5.0 * 10
9The seed culture fluid of cfu/ml, Bacillus acidi lactici bacterium number 〉=5.0 * 10
9The seed culture fluid of cfu/ml;
Bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) the Weiss et al) preparation of CGMCC 1.1480 seed culture fluids: with bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) CGMCC 1.1480 is inoculated in the fluid nutrient medium under aseptic condition, the content of each component in 1.0L distilled water is in the described fluid nutrient medium: skimmed milk power 100g, nature pH value, cultivated 24～48 hours, and obtained bacillus bulgaricus bacterium number 〉=5.0 * 10 for 28 ℃～32 ℃
9The seed culture fluid of cfu/ml;
(2) preparation of many strain microbial composite beverages preparation:
The Bacillus natto that respectively step (1) is prepared (
Bacillus natto Swamura) ACCC 10614, Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092, Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855 and bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) seed culture fluid of CGMCC 1.1480 by its percetage by weight is: the seed culture fluid 25%～35% of Bacillus natto, the seed culture fluid 10%～15% of Bacillus acidi lactici, the seed culture fluid 15%～5% of Bifidobacterium, the seed culture fluid 15%～25% of streptococcus thermophilus, the seed culture fluid 10%～15% of bacillus bulgaricus, under aseptic condition, be inoculated into respectively in the same fluid nutrient medium, inoculum concentration is 1 ﹪～2 ﹪ of culture medium weight, the content of each component in 1.0L distilled water is in the described fluid nutrient medium: sterilization brown sugar 50.0g, sterilization honey 10.0g, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentation tank and zymotic fluid is under 5% the condition at oxygen dissolved, 28 ℃～32 ℃ airtight leaving standstill were cultivated 10～15 days, make the pH value drop to 3.0～3.5, obtain many strain microbial composite beverages preparation.
Bacterial classification function used in the present invention is as follows:
(1) Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092, promote the absorption of the nutriments such as protein, monose and calcium, magnesium, produce a large amount of benefit materials such as vitamin B complex; Make the formation of gut flora that useful variation occur, improve the human gastrointestinal tract function, recover colony balance in the human body intestinal canal, form the antibacterial biological barrier, safeguard health; Suppress the breeding of spoilage organisms, clear up the toxin that spoilage organisms produces, remove enteron aisle rubbish.Suppress cholesterol absorption, reducing blood lipid, hypotensive activity; Immunoregulation effect strengthens body immunity and resistance; Antitumor, pre-anti-cancer effect; Improve the SOD enzyme activity, eliminate human free radical, anti-ageing, the effect of promoting longevity of tool; Effectively prevention female genitourinary system bacterium infects.Control toxins in human body level, the protection liver also strengthens detoxifcation, the functions of expelling toxin of liver.
(2) Bifidobacterium (
BifidobacteriumSp) ACCC 11054, safeguard enteron aisle normal bacteria colony balance, suppress the growth of pathogen, synthetic vitamin, amino acid and raising body produce vitamin B1, B2, B6, B12 and alanine, valine, aspartic acid and threonine etc. to the absorption of calcium ion in enteron aisle.Have the Blood Cholesterol of reduction level, prevent and treat high blood, strengthen the human immunologic function, prevent antibiotic side effect, anti-ageing, promote longevity.
(3) Bacillus natto (
Bacillus natto Swamura) ACCC 10614, produce acid, the regulating intestinal canal flora, enhancing human body intestinal canal cellular immunity is put and is answered, and can generate multiple protein enzyme (particularly alkali protease), carbohydrase, lipase, amylase, some complicated carbohydrate of degrading plant food kind.
(4) streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855 and bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) CGMCC 1.1480 is Healthy People normal intestinal floras, can in human body intestinal canal, grow, breed, can directly replenish the human normal physiological bacteria, adjust the gut flora balance, inhibition is also removed the bacterium that in the enteron aisle people is had potential hazard.
In many strain microbial composite beverages preparation provided by the invention: Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092, Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855 and bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) CGMCC 1.1480 bacterial classifications belong to Lactobacillus together, and biological property is similar, and Bacillus natto (
Bacillus natto Swamura) ACCC 10614 and above-mentioned 4 kinds of bacterial classifications have the product acid function, be under the acid condition of 3.0-3.5 at pH value, it can form metastable resting state, make each bacterial classification can mutually not restrict counteracting, simultaneously, it utilizes the synthetic material of other microorganisms Bacillus natto and Bacillus acidi lactici, supporting the activity of other microorganisms, form symbiosis with other bacterial classification, bringing into play leading and central role, in stable condition to guarantee many strain microbial composite beverages preparation active liquid provided by the invention, function diversification.
Beneficial effect of the present invention: the present invention utilizes many strain microbial composite beverages preparation of five kinds of beneficial microbe strain preparations, after entering human body, not only can give full play to function separately, promote the propagation of beneficial bacterium in the body, suppress the breeding of harmful bacteria, can also provide essential material (to comprise growth factor for growth and the Function of other human body beneficial microorganism (comprising 5 kinds of bacterial classifications used in the present invention), carbohydrate, amino acid etc.), microecological balance in balance the body intestines and stomach and the reproductive system, make human body be in microecological balance, whet the appetite, promote digestion, prevent simultaneously the utmost point chronic disease in the various intestines and stomach.
The specific embodiment
The present invention is described in further detail below in conjunction with following specific embodiment, but the present invention not only is confined to following examples.
Embodiment 1
A kind of preparation method of many strain microbial composite beverages preparation may further comprise the steps:
(1) preparation of seed culture fluid:
Bacillus natto (
Bacillus natto Swamura) preparation of ACCC 10614 seed culture fluids: Bacillus natto is inoculated in the fluid nutrient medium under aseptic condition, described fluid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, the pH value transfers to 7.0, under gnotobasis, inoculate, cultivated 48 hours, and obtained Bacillus natto bacterium number 〉=5.0 * 10 for 28 ℃～32 ℃
9The seed culture fluid of cfu/ml;
Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855, Bacillus acidi lactici (
Lactococcus lactis) preparation of seed culture fluid of ACCC 11092: respectively with Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855, Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092 is inoculated in the fluid nutrient medium under aseptic condition, described fluid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 10.1g, beef extract 10.0g, dusty yeast 5.0g, glucose 5.0g, sodium acetate 5.0g, 28 ℃～32 ℃ single bacterial classifications were cultivated 24～48 hours, obtained respectively Bifidobacterium bacterium number 〉=5.0 * 10
9The Bifidobacterium seed nutrient solution of cfu/ml, streptococcus thermophilus bacterium number 〉=5.0 * 10
9The streptococcus thermophilus seed culture fluid of cfu/ml, Bacillus acidi lactici bacterium number 〉=5.0 * 10
9The Bacillus acidi lactici seed nutrient solution of cfu/ml;
Bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) the Weiss et al) preparation of CGMCC 1.1480 seed culture fluids: with bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) CGMCC 1.1480 is inoculated in the fluid nutrient medium under aseptic condition, described fluid nutrient medium is: the content of each component in 1.0L distilled water is: the content of each component in 1.0L distilled water is: skimmed milk power 100g, nature pH value, under aseptic condition, inoculate, cultivated 24～48 hours, and obtained bacillus bulgaricus bacterium number 〉=5.0 * 10 for 28 ℃～32 ℃
9The seed culture fluid of cfu/ml.
(2) preparation of many strain microbial composite beverages preparation:
The seed culture fluid of each bacterial classification that respectively step (1) is prepared by its percetage by weight is: the seed culture fluid 25% of Bacillus natto, the seed culture fluid 15% of Bacillus acidi lactici, the seed culture fluid 20% of Bifidobacterium, the seed culture fluid 25% of streptococcus thermophilus, the seed culture fluid 15% of bacillus bulgaricus, under aseptic condition, be inoculated into respectively in the same fluid nutrient medium, inoculum concentration is 1 ﹪～2 ﹪ of culture medium weight, described fluid nutrient medium is: the content of each component in 1.0L distilled water is: sterilization brown sugar 50.0g, sterilization honey 10.0g, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentation tank and zymotic fluid is under 5% the condition at oxygen dissolved, 28 ℃～32 ℃ airtight standing for fermentation 10～15 days, make the pH value drop to 3.0～3.5, obtain many strain microbial composite beverages preparation.
After measured, this many strain microbial composite beverages preparation golden yellow color, the pH value is 3.0-3.5, wherein Bacillus natto (
Bacillus natto Swamura) the bacterium number 〉=5.0 * 10 of ACCC 10614
9Cfu/ml, Bacillus acidi lactici (
Lactococcus lactis) the bacterium number 〉=5.0 * 10 of ACCC 11092
9Cfu/ml, Bifidobacterium (
BifidobacteriumSp) the bacterium number of ACCC 11054 〉=5.0 * 10
9Cfu/ml, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) the bacterium number of CGMCC 1.1855 〉=5.0 * 10
9Cfu/m, bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) the bacterium number of CGMCC 1.1480 〉=5.0 * 10
9Cfu/ml.
Embodiment 2
A kind of preparation method of many strain microbial composite beverages preparation may further comprise the steps:
(1) preparation of seed culture fluid:
Employing makes seed culture fluid with the identical method of embodiment 1;
(2) preparation of many strain microbial composite beverages preparation:
The seed culture fluid of each bacterial classification that respectively step (1) is prepared, according to Bacillus natto (
Bacillus natto Swamura) ACCC10614, Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092, Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855 and bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) seed culture fluid of CGMCC 1.1480 by its percetage by weight is: the seed culture fluid 35% of Bacillus natto, the seed culture fluid 15% of Bacillus acidi lactici, the seed culture fluid 25% of Bifidobacterium, the seed culture fluid 15% of streptococcus thermophilus, the seed culture fluid 10% of bacillus bulgaricus, under aseptic condition, be inoculated into respectively in the same fluid nutrient medium, inoculum concentration is 1 ﹪～2 ﹪ of culture medium weight, described fluid nutrient medium is: the content of each component in 1.0L distilled water is: sterilization brown sugar 50.0g, sterilization honey 10.0g, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentation tank and zymotic fluid is under 5% the condition at oxygen dissolved, 28 ℃～32 ℃ airtight leaving standstill were cultivated 10～15 days, make the pH value drop to 3.0～3.5, obtain many strain microbial composite beverages preparation.
After measured, this many strain microbial composite beverages preparation golden yellow color, the pH value is 3.0～3.5, wherein Bacillus natto (
Bacillus natto Swamura) the bacterium number 〉=5.0 * 10 of ACCC 10614
9Cfu/ml, Bacillus acidi lactici (
Lactococcus lactis) the bacterium number 〉=5.0 * 10 of ACCC 11092
9Cfu/ml, Bifidobacterium (
BifidobacteriumSp) the bacterium number of ACCC 11054 〉=5.0 * 10
9Cfu/ml, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) the bacterium number of CGMCC 1.1855 〉=5.0 * 10
9Cfu/m, bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) the bacterium number of CGMCC 1.1480 〉=5.0 * 10
9Cfu/ml.
Embodiment 3
A kind of preparation method of many strain microbial composite beverages preparation may further comprise the steps:
(1) preparation of seed culture fluid:
Employing makes seed culture fluid with the identical method of embodiment 1;
(2) preparation of many strain microbial composite beverages preparation:
The seed culture fluid of each bacterial classification that respectively step (1) is prepared, according to Bacillus natto (
Bacillus natto Swamura) ACCC10614, Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092, Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855 and bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) seed culture fluid of CGMCC 1.1480 by its weight ratio is: the seed culture fluid 30% of Bacillus natto, the seed culture fluid 13% of Bacillus acidi lactici, the seed culture fluid 25% of Bifidobacterium, the seed culture fluid 20% of streptococcus thermophilus, the seed culture fluid 12% of bacillus bulgaricus, under aseptic condition, be inoculated into respectively in the same fluid nutrient medium, inoculum concentration is 1 ﹪～2 ﹪ of culture medium weight, described fluid nutrient medium is: the content of each component in 1.0L distilled water is: sterilization brown sugar 50.0g, sterilization honey 10.0g, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentation tank and zymotic fluid is under 5% the condition at oxygen dissolved, 28 ℃～32 ℃ airtight leaving standstill were cultivated 10～15 days, make the pH value drop to 3.0～3.5, obtain many strain microbial composite beverages preparation.
After measured, this many strain microbial composite beverages preparation golden yellow color, the pH value is 3.0～3.5, wherein Bacillus natto (
Bacillus natto Swamura) the bacterium number 〉=5.0 * 10 of ACCC 10614
9Cfu/ml, Bacillus acidi lactici (
Lactococcus lactis) the bacterium number 〉=5.0 * 10 of ACCC 11092
9Cfu/ml, Bifidobacterium (
BifidobacteriumSp) the bacterium number of ACCC 11054 〉=5.0 * 10
9Cfu/ml, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) the bacterium number of CGMCC 1.1855 〉=5.0 * 10
9Cfu/m, bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) the bacterium number of CGMCC 1.1480 〉=5.0 * 10
9Cfu/ml.
Claims (2)
1. strain microbial composite beverage preparation more than a kind is characterized in that: it be with Bacillus natto (
Bacillus natto Swamura) ACCC 10614, Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092, Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855 and bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) CGMCC 1.1480 carries out respectively single bacterial classification and cultivates the seed culture fluid that obtains and be inoculated in the same fluid nutrient medium under aseptic condition, inoculum concentration is 1 ﹪～2 ﹪ of culture medium weight, the content of each component in 1.0L distilled water is in the described fluid nutrient medium: sterilization brown sugar 50.0g, sterilization honey 10.0g, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentation tank and zymotic fluid is under 5% the condition at oxygen dissolved, 28 ℃～32 ℃ airtight leaves standstill that to cultivate 10～15 days pH values to nutrient solution be 3～3.5 to obtain, the seed culture fluid of wherein said each bacterial classification by its percetage by weight is: the seed culture fluid 25%～35% of Bacillus natto, the seed culture fluid 10%～15% of Bacillus acidi lactici, the seed culture fluid 15%～25% of Bifidobacterium, the seed culture fluid 15%～25% of streptococcus thermophilus, the seed culture fluid 10%～15% of bacillus bulgaricus, Bacillus natto in the described preparation (
Bacillus natto Swamura) the bacterium number 〉=5.0 * 10 of ACCC 10614
9Cfu/ml, Bacillus acidi lactici (
Lactococcus lactis) the bacterium number 〉=5.0 * 10 of ACCC 11092
9Cfu/ml, Bifidobacterium (
BifidobacteriumSp) the bacterium number of ACCC 11054 〉=5.0 * 10
9Cfu/ml, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) the bacterium number of CGMCC 1.1855 〉=5.0 * 10
9Cfu/ml, bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) the bacterium number of CGMCC 1.1480 〉=5.0 * 10
9Cfu/ml.
2. the preparation method of many strain microbial composite beverages preparation according to claim 1 is characterized in that, may further comprise the steps:
(1) preparation of seed culture fluid:
Bacillus natto (
Bacillus natto Swamura) preparation of ACCC 10614 seed culture fluids: with Bacillus natto (
Bacillus natto Swamura) ACCC 10614 is inoculated in the fluid nutrient medium under aseptic condition, described fluid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, the pH value transfers to 7.0, cultivated 48 hours, and obtained Bacillus natto bacterium number 〉=5.0 * 10 for 28 ℃～32 ℃
9The seed culture fluid of cfu/ml;
Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855, Bacillus acidi lactici (
Lactococcus lactis) preparation of seed culture fluid of ACCC 11092: respectively with Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855, Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092 is inoculated in the fluid nutrient medium under aseptic condition, the content of each component in 1.0L distilled water is in the described fluid nutrient medium: peptone 10.1g, beef extract 10.0g, dusty yeast 5.0g, glucose 5.0g, sodium acetate 5.0g, 28 ℃～32 ℃ single bacterial classifications were cultivated 24～48 hours, obtained respectively Bifidobacterium bacterium number 〉=5.0 * 10
9The seed culture fluid of cfu/ml, streptococcus thermophilus bacterium number 〉=5.0 * 10
9The seed culture fluid of cfu/ml, Bacillus acidi lactici bacterium number 〉=5.0 * 10
9The seed culture fluid of cfu/ml;
Bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) the Weiss et al) preparation of CGMCC 1.1480 seed culture fluids: with bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) CGMCC 1.1480 is inoculated in the fluid nutrient medium under aseptic condition, the content of each component in 1.0L distilled water is in the described fluid nutrient medium: skimmed milk power 100g, nature pH value, cultivated 24～48 hours, and obtained bacillus bulgaricus bacterium number 〉=5.0 * 10 for 28 ℃～32 ℃
9The seed culture fluid of cfu/ml;
(2) preparation of many strain microbial composite beverages preparation:
The Bacillus natto that respectively step (1) is prepared (
Bacillus natto Swamura) ACCC 10614, Bacillus acidi lactici (
Lactococcus lactis) ACCC 11092, Bifidobacterium (
BifidobacteriumSp) ACCC 11054, streptococcus thermophilus (
Streptococcus thermophilusOrla-Jensen) CGMCC 1.1855 and bacillus bulgaricus (
Lactobacillus delbrueckiiSubsp.
Bulgaricus(crla-Jensen) Weiss et al) seed culture fluid of CGMCC 1.1480 by its percetage by weight is: the seed culture fluid 25%～35% of Bacillus natto, the seed culture fluid 10%～15% of Bacillus acidi lactici, the seed culture fluid 15%～25% of Bifidobacterium, the seed culture fluid 15%～25% of streptococcus thermophilus, the seed culture fluid 10%～15% of bacillus bulgaricus, under aseptic condition, be inoculated into respectively in the same fluid nutrient medium, inoculum concentration is 1 ﹪～2 ﹪ of culture medium weight, the content of each component in 1.0L distilled water is in the described fluid nutrient medium: sterilization brown sugar 50.0g, sterilization honey 10.0g, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentation tank and zymotic fluid is under 5% the condition at oxygen dissolved, 28 ℃～32 ℃ airtight leaving standstill were cultivated 10～15 days, make the pH value drop to 3.0～3.5, obtain many strain microbial composite beverages preparation.
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