CN107698694A - A kind of Ginger Polysaccharide and its extracting method and application - Google Patents
A kind of Ginger Polysaccharide and its extracting method and application Download PDFInfo
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- CN107698694A CN107698694A CN201711185067.7A CN201711185067A CN107698694A CN 107698694 A CN107698694 A CN 107698694A CN 201711185067 A CN201711185067 A CN 201711185067A CN 107698694 A CN107698694 A CN 107698694A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The invention provides a kind of Ginger Polysaccharide and its extracting method and application, the extracting method comprises the following steps：(1) low temperature extracts：Residue of the ginger after squeezing the juice is placed in refluxing extraction equipment, Extraction solvent is added, is extracted；(2) thickening filtration：Extract solution is sieved, concentrated, filtered, purified, obtains refined filtration liquid；(3) primary sedimentation：Ethanol is added into step (2) the refined filtration liquid and is centrifuged, precipitation is dried under reduced pressure；(4) secondary precipitation：Ethanol is added in the supernatant obtained to step (3) centrifugation and is centrifuged, and precipitation is dried under reduced pressure, the Ginger Polysaccharide is made.Low temperature extractive technique, ethanol precipitation and centrifugally operated are combined by the Ginger Polysaccharide extracting method of the present invention, synergy, significantly eliminates the content of starch in Ginger Polysaccharide, the Ginger Polysaccharide dissolubility of extraction is good, solution clear, hydroscopicity and moisturizing rate are respectively provided with and are obviously improved.
Description
Technical field
The invention belongs to the integrated application field of ginger resource, it is related to a kind of Ginger Polysaccharide and its extracting method and application,
More particularly to a kind of soluble Ginger Polysaccharide and its extracting method and application.
Background technology
At present, most of cosmetics are mainly used in the maintenance of skin and healthy hair on the market, when in skin be particularly cuticula
In water content when reaching 10-20%, skin could soft, smooth, delicate and high resilience.However, low temperature, being air-dried, being purple
Outside line irradiation, age growth can cause cuticle loss, make the barrier function damage of cuticula, and hydration status change, caused
Skin dehydration.Therefore, the key for keep moisture of skin, preventing skin aging from being beauty and skin care, exploitation moisture-keeping cosmetics are skin cares
The eternal theme in field.
From the polysaccharide of natural plants, have no toxic side effect, be good with skin substantivity.Hydroxyl, carboxyl in polysaccharide molecule
With other polar groups by hydrogen bond and water molecules, and it is woven into a mesh structure, plays water retention.In extracellular matrix
In, polysaccharide forms extracellular gelatinised matrix with the fibrin in skin, and moisture is provided for skin.In addition, polysaccharide can also be in skin
Skin surface forms one layer of uniform film, reduces the evaporation of skin surface moisture, and moisture permeates into cuticula from substrate microstructure, lured
The further aquation of cuticula is led, so as to complete moisturizing effect.The water imbibition and film forming of polysaccharide, make it have good moisturizing effect
Fruit.Being applied to the polysaccharide NMF in cosmetics at present includes hyaluronic acid chitin and its derivative, plant polyose extraction
Thing and heparin etc..
Ginger is a kind of Zingiber zingiber herbaceos perennial, originates in subtropical zone, and ginger is the new fresh quench stem of ginger, has medicine concurrently
Eat homology.Research finds, contains multiple nutritional components in ginger, including volatile oil, fat oil, pungent element, resin, protein,
Vitamin (mainly nicotinic acid and vitamin A), pentosan, starch and mineral element, wherein ginger oil content is 1-3%, and fat contains
Measure as 6-8%, content of starch highest, account for Jiang Ganchong 40-60%, starch is substantially also a kind of polysaccharide, and its molecule
Amount is very big, and starch easily produces gelatinization phenomenon at a certain temperature, so appropriate low temperature is more conducive to extract higher purity
(non-starch) polysaccharide.In addition, the acid of ginger mostlys come from volatile oil and gingerol constituents, limit to a certain extent
The application of ginger polysaccharide.
CN104672340B the Granted publications a kind of extraction process of Ginger Polysaccharide and its application of extract, the technique will
Ginger is by cutting into slices, dry, water extraction, removal of impurities, dissolving, after alcohol precipitation, obtains Ginger Polysaccharide.However, the technique has used chloroform, just
The organic solvents such as butanol, organic residue is more, security is poor, in addition, the content of starch in ginger does not significantly reduce, ginger is more
The recovery rate of sugar is relatively low.
In the extraction process of polysaccharide, secondary precipitation often is carried out to polysaccharide using alcohols, to improve the purity of polysaccharide.So
And it is poor containing macromolecular polysaccharide, these composition dissolubilities such as starch, colloid in ginger, use alcohols carry out precipitation can cause it is molten
Liquid becomes cloudy, and limits application of the extract in cosmetics.
Therefore it provides a kind of organic residue is few, recovery rate is high, the simple Ginger Polysaccharide extracting method of technique so that extraction
Ginger Polysaccharide dissolubility it is good, safe, clarity is good, cosmetic field have great importance with before wide market
Scape.
The content of the invention
For overcome the deficiencies in the prior art, the present invention provides a kind of Ginger Polysaccharide and its extracting method and application, solves
The Ginger Polysaccharide content of starch of prior art extraction is high, and (phenomenon is shown as：Solution low temperature easily becomes cloudy), dissolubility difference ask
Topic, the soluble Ginger Polysaccharide of extraction, moistening effect is notable, available for moisture-keeping cosmetics are prepared, has a good application prospect.
In a first aspect, the invention provides a kind of extracting method of Ginger Polysaccharide, comprise the following steps：
(1) low temperature extracts：Residue of the ginger after squeezing the juice is placed in refluxing extraction equipment, Extraction solvent is added, enters
Row extraction；
(2) thickening filtration：Extract solution is sieved, concentrated, filtered, purified, obtains refined filtration liquid；
(3) primary sedimentation：Ethanol is added into step (2) the refined filtration liquid and is centrifuged, precipitation is dried under reduced pressure；
(4) secondary precipitation：Ethanol is added in the supernatant obtained to step (3) centrifugation and is centrifuged, and precipitation is carried out
It is dried under reduced pressure, the Ginger Polysaccharide is made.
In the present invention, low temperature extraction refers to that the temperature of extraction is less than 90 DEG C, and technique energy is reduced using low temperature extractive technique
Consumption, and centrifugally operated is carried out during ethanol precipitation twice, significantly eliminate the content of starch in Ginger Polysaccharide, the life of extraction
Ginger polysaccharide dissolubility is good, solution clear, and hydroscopicity and moisturizing rate are respectively provided with compared with tremella polysaccharides and be obviously improved.
Preferably, the solid-liquid ratio of step (1) residue and the Extraction solvent is 1:(2-5), such as can be 1:2、
1:3、1:4 or 1:5, preferably 1:3.
Preferably, step (1) described Extraction solvent is purified water.
Preferably, the temperature of step (1) described extraction is 50-90 DEG C, for example, can be 50 DEG C, 52 DEG C, 55 DEG C, 58 DEG C,
60 DEG C, 61 DEG C, 62 DEG C, 65 DEG C, 68 DEG C, 70 DEG C, 72 DEG C, 75 DEG C, 78 DEG C, 80 DEG C, 82 DEG C, 85 DEG C, 88 DEG C or 90 DEG C, it is preferably
61℃。
In the present invention, the temperature of the extraction is less than 90 DEG C, not only reduces process energy consumption, and is advantageous to give birth in extraction
The starch of dissolubility difference is removed during ginger polysaccharide, improves the yield of Ginger Polysaccharide.
Preferably, the time of step (1) described extraction is 1-3h, such as can be 1h, 1.5h, 2h, 2.5h or 3h, preferably
For 2h.
Preferably, the number of step (1) described extraction is 1-3 times, such as can be 1 time, 2 times or 3 times, preferably 2 times.
Preferably, the mesh number of step (2) described sieving is 100-300 mesh, for example, can be 100 mesh, 120 mesh, 140 mesh,
160 mesh, 180 mesh, 200 mesh, 220 mesh, 240 mesh, 260 mesh, 280 mesh or 300 mesh, preferably 200 mesh.
Preferably, step (2) concentration uses reverse osmosis membrane.
Preferably, the pressure of step (2) described concentration is 5-30bar, for example, can be 5bar, 8bar, 10bar,
12bar, 15bar, 18bar, 20bar, 22bar, 25bar, 28bar or 30bar, preferably 20bar.
Preferably, step (2) filtering uses membrane filtration system.
Preferably, the aperture of the membrane filtration system is 0.1-0.5 μm, such as can be 0.1 μm, 0.22 μm, 0.45 μm
Or 0.5 μm, preferably 0.22 μm.
Preferably, the pressure of the membrane filtration system is 1-5bar, for example, can be 1bar, 2bar, 3bar, 4bar or
5bar, preferably 3bar.
Preferably, step (2) purifying uses large aperture resin.
Preferably, the large aperture resin is D101 and/or AB-8 resin columns.
Preferably, the eluent of step (2) described purifying is ethanol water.
Preferably, the concentration of the ethanol water is 10-20%, for example, can be 10%, 13%, 15%, 18% or
20%, preferably 15%.
Preferably, the speed of step (3) described centrifugation is 3000-5000r/min, for example, can be 3000r/min,
3500r/min, 4000r/min, 4500r/min or 5000r/min, preferably 4000r/min.
Preferably, the time of step (3) described centrifugation is 5-20min, for example, can be 5min, 8min, 10min,
13min, 17min, 20min, preferably 10min.
Preferably, the speed of step (4) described centrifugation is 3000-5000r/min, for example, can be 3000r/min,
3500r/min, 4000r/min, 4500r/min or 5000r/min, preferably 4000r/min.
Preferably, the time of step (4) described centrifugation is 5-20min, such as can be 5min, 8min, 10min, 13min
Or 20min, preferably 10min.
As optimal technical scheme, the invention provides a kind of extracting method of Ginger Polysaccharide, comprise the following steps：
(1) low temperature extracts：Residue of the ginger after squeezing the juice is placed in refluxing extraction equipment, is 1 by solid-liquid ratio:(2-
5) Extraction solvent is added, is extracted 1-3 times at 50-90 DEG C, each 1-3h；
(2) thickening filtration：After extract solution is crossed into 100-300 mesh sieves, reverse osmosis concentration is carried out under 5-30bar pressure,
Filtered under 1-5bar using 0.1-0.5 μm of membrane filtration system, upper D101 and/or AB-8 resin columns are purified, and use 10-
20% ethanol solution elutes, and obtains refined filtration liquid；
(3) primary sedimentation：It is eventually 30-80% to volumetric concentration that ethanol is added into step (2) the refined filtration liquid,
5-20min is centrifuged under 3000-5000r/min, precipitation is dried under reduced pressure；
(4) secondary precipitation：Ethanol is added in obtained supernatant to final volume concentration 70- to described centrifuge of step (3)
95%, 5-20min is centrifuged under 3000-5000r/min, precipitation is dried under reduced pressure, the Ginger Polysaccharide is made.
Second aspect, the invention provides the Ginger Polysaccharide that a kind of extracting method as described in relation to the first aspect is extracted to obtain.
The third aspect, it is used to prepare cosmetics the invention provides a kind of Ginger Polysaccharide as described in second aspect.
Compared with prior art, the present invention has the advantages that：
(1) present invention carries out the extraction of Ginger Polysaccharide using low temperature extractive technique, is carried out during ethanol precipitation twice
Centrifugally operated, the Ginger Polysaccharide recovery rate of this method are up to 34.5%, and the Ginger Polysaccharide content of starch of extraction is low, solution clarification
Transparent is in faint yellow；
(2) compared with tremella polysaccharides, hydroscopicity and moisturizing rate obtain the Ginger Polysaccharide extracted using the extracting method of the present invention
To being obviously improved, cosmetic field can be applied to as effective moisturizing ingredient；
(3) each step Synergistic of method of the invention, recovery rate is high, reliable and stable.
Brief description of the drawings
Fig. 1 is polysaccharide hydroscopicity time changing curve；
Fig. 2 is polysaccharide powder moisturizing rate time changing curve；
Fig. 3 is polysaccharide solution moisturizing rate time changing curve.
Embodiment
For the technological means and its effect that the present invention is taken is expanded on further, with reference to embodiments with accompanying drawing to this hair
It is bright to be further described.It is understood that embodiment described herein is used only for explaining the present invention, rather than
Limitation of the invention.
In the examples where no specific technique or condition is specified, according to the technology or condition described by document in the art,
Or carried out according to product description.Agents useful for same or the unreceipted production firm person of instrument, be can be by regular channel commercially available from
The conventional products of acquisition.
The extraction of the Ginger Polysaccharide of embodiment 1 (I)
(1) low temperature extracts：Residue of the ginger after squeezing the juice is placed in refluxing extraction equipment, is 1 by solid-liquid ratio:3 add
Enter purified water, extracted 2 times at 61 DEG C, each 2h；
(2) thickening filtration：After extract solution is crossed into 200 mesh sieves, reverse osmosis concentration is carried out under 20bar pressure, under 3bar
Filtered using 0.22 μm of membrane filtration system, upper D101 resin columns are purified, and are eluted with 15% ethanol solution, are obtained refined filtration
Liquid；
(3) primary sedimentation：Ethanol is added into step (2) the refined filtration liquid to final concentration of 50%, under 4000r/min
10min is centrifuged, precipitation is dried under reduced pressure；
(4) secondary precipitation：Ethanol is added in obtained supernatant to final concentration of 80% to described centrifuge of step (3),
10min is centrifuged under 4000r/min, precipitation is dried under reduced pressure, the Ginger Polysaccharide is made.
The extraction of the Ginger Polysaccharide of embodiment 2 (II)
(1) low temperature extracts：Residue of the ginger after squeezing the juice is placed in refluxing extraction equipment, is 1 by solid-liquid ratio:3 add
Enter purified water, extracted 2 times at 61 DEG C, each 1.5h；
(2) thickening filtration：After extract solution is crossed into 250 mesh sieves, reverse osmosis concentration is carried out under 15bar pressure, under 4bar
Filtered using 0.45 μm of membrane filtration system, upper AB-8 resin columns are purified, and are eluted with 12% ethanol solution, are obtained refined filtration
Liquid；
(3) primary sedimentation：Ethanol is added into step (2) the refined filtration liquid to final concentration of 40%, under 4500r/min
15min is centrifuged, precipitation is dried under reduced pressure；
(4) secondary precipitation：Ethanol is added in obtained supernatant to final concentration of 75% to described centrifuge of step (3),
15min is centrifuged under 4500r/min, precipitation is dried under reduced pressure, the Ginger Polysaccharide is made.
The extraction of the Ginger Polysaccharide of embodiment 3 (III)
(1) low temperature extracts：Residue of the ginger after squeezing the juice is placed in refluxing extraction equipment, is 1 by solid-liquid ratio:4 add
Enter purified water, extracted 2 times at 61 DEG C, each 2.5h；
(2) thickening filtration：After extract solution is crossed into 100 mesh sieves, reverse osmosis concentration is carried out under 25bar pressure, under 2bar
Filtered using 0.45 μm of membrane filtration system, upper D101 resin columns are purified, and are eluted with 18% ethanol solution, are obtained refined filtration
Liquid；
(3) primary sedimentation：Ethanol is added into step (2) the refined filtration liquid to final concentration of 60%, under 3500r/min
8min is centrifuged, precipitation is dried under reduced pressure；
(4) secondary precipitation：Ethanol is added in obtained supernatant to final concentration of 90% to described centrifuge of step (3),
8min is centrifuged under 3500r/min, precipitation is dried under reduced pressure, the Ginger Polysaccharide is made.
The extraction of the Ginger Polysaccharide of embodiment 4 (IV)
(1) low temperature extracts：Residue of the ginger after squeezing the juice is placed in refluxing extraction equipment, is 1 by solid-liquid ratio:2 add
Enter purified water, extracted 1 time at 50 DEG C, each 1h；
(2) thickening filtration：After extract solution is crossed into 300 mesh sieves, reverse osmosis concentration is carried out under 5bar pressure, is adopted under 5bar
Filtered with 0.1 μm of membrane filtration system, upper AB-8 resin columns are purified, and are eluted with 10% ethanol solution, are obtained refined filtration liquid；
(3) primary sedimentation：Ethanol is added into step (2) the refined filtration liquid to final concentration of 30%, under 5000r/min
20min is centrifuged, precipitation is dried under reduced pressure；
(4) secondary precipitation：Ethanol is added in obtained supernatant to final concentration of 70% to described centrifuge of step (3),
20min is centrifuged under 5000r/min, precipitation is dried under reduced pressure, the Ginger Polysaccharide is made.
The extraction of the Ginger Polysaccharide of embodiment 5 (V)
(1) low temperature extracts：Residue of the ginger after squeezing the juice is placed in refluxing extraction equipment, is 1 by solid-liquid ratio:5 add
Enter purified water, extracted 3 times at 90 DEG C, each 3h；
(2) thickening filtration：After extract solution is crossed into 100 mesh sieves, reverse osmosis concentration is carried out under 30bar pressure, under 1bar
Filtered using 0.5 μm of membrane filtration system, upper D101 resin columns are purified, and are eluted with 20% ethanol solution, are obtained refined filtration liquid；
(3) primary sedimentation：Ethanol is added into step (2) the refined filtration liquid to final concentration of 50%, under 3000r/min
5min is centrifuged, precipitation is dried under reduced pressure；
(4) secondary precipitation：Ethanol is added in obtained supernatant to final concentration of 95% to described centrifuge of step (3),
5min is centrifuged under 3000r/min, precipitation is dried under reduced pressure, the Ginger Polysaccharide is made.
Comparative example 1
Compared with Example 1, purified in the extraction process of Ginger Polysaccharide without resin column, other preparation process are with implementing
Example 1 is identical.
Comparative example 2
Compared with Example 1, without secondary precipitation in Ginger Polysaccharide extraction process.
Comparative example 3
Compared with Example 1, the temperature extracted in Ginger Polysaccharide extraction process is 30 DEG C.
Comparative example 4
Compared with Example 1, the temperature extracted in Ginger Polysaccharide extraction process is 100 DEG C.
The recovery rate and polyoses content of Ginger Polysaccharide
The recovery rate of the Ginger Polysaccharide of each embodiment and comparative example is calculated, polyoses content is determined, compares Ginger Polysaccharide solution
Clarity, the results are shown in Table 1.
Recovery rate, polyoses content and the clarity of solution of the Ginger Polysaccharide of table 1
Embodiment 1-5 extracting method Rational Parameters, matched somebody with somebody under 50-90 DEG C of Extracting temperature using ethanol precipitation technology twice
Centrifugally operated is closed, Ginger Polysaccharide recovery rate is up to 34.5%, and the Ginger Polysaccharide content of starch of extraction is low, and dissolubility is good, is prepared
Solution clear, in weak yellow liquid.
Compared with Example 1, comparative example 1 purifies in extraction process without resin column, and the impurity component of colour generation compares
It is more so that the solution of preparation is in dark brown liquid, limits the use of polysaccharide to a certain degree；Comparative example 2 in extraction process only
Carry out an ethanol precipitation, it is impossible to effectively reduce the content of starch in Ginger Polysaccharide, the clarity of solution of preparation is poor, presents milky white
The suspended state of color；Comparative example 3-4 Extracting temperature is unreasonable, reduces recovery rate.
External moisture absorption, moisturizing experiment
It is 81% (ammonium sulfate saturated solution) that the Ginger Polysaccharide of the drying of embodiment and comparative example is positioned over into relative humidity
Close drying device in, weighed in 0h, 4h, 8h, 12h, 24h, 48h.Hydroscopicity is calculated according to below equation：
Hydroscopicity %=(Wn-Wo)/Wo×100
Wherein, WnFor the example weight of n-th hour, WoFor the initial weight 1.0g of sample.
After moisture absorption 48h, example weight (W is weighed48), calculate sample moisture content W1, as initial water content.Sample is put
It is placed in the close drying device equipped with silica gel, moisturizing rate is calculated according to below equation：
Moisturizing rate %=(W48-Wm)/W1×100
Wherein, WmFor the example weight of m hours, W48For the example weight of the 48th hour, W1For original state water content.
Ginger Polysaccharide is configured to the solution that concentration is 0.2% and 1%, solution is positioned over the drier equipped with silica gel
In, the moisturizing rate of calculating moisturizing rate Ginger Polysaccharide solution.
It the results are shown in Table 2 and Fig. 1, Fig. 2 and Fig. 3.
The hydroscopicity (48h) and moisturizing rate (24h) of the Ginger Polysaccharide of table 2
Compared with conventional polysaccharide NMF tremella polysaccharides, embodiment 1-5 Ginger Polysaccharide hydroscopicity is strong, higher than 35%.
As shown in figure 1, the Ginger Polysaccharide of embodiment 1 and the hydroscopicity of tremella polysaccharides reach maximum, but the suction of tremella polysaccharides in 8h
Wet rate is less than Ginger Polysaccharide.
Compared with conventional polysaccharide NMF tremella polysaccharides, embodiment 1-5 Ginger Polysaccharide powder and Ginger Polysaccharide solution
Stronger moisturizing rate is respectively provided with, the moisturizing rate of Ginger Polysaccharide powder is more than 20%, and the moisturizing rate of Ginger Polysaccharide solution is more than 55%.
As shown in Fig. 2 in initial 8 hours, the moisturizing rate of tremella polysaccharides is higher than Ginger Polysaccharide, but the duration is shorter, 24 hours
Afterwards, the moisturizing rate of tremella polysaccharides is reduced to 13.1%, the only half of Ginger Polysaccharide；As shown in figure 3, the guarantor of Ginger Polysaccharide solution
Wet rate was above tremella polysaccharides at 24 hours in minute.
In summary, a kind of extracting method of Ginger Polysaccharide provided by the invention uses low temperature extractive technique, and twice
Centrifugally operated is carried out during ethanol precipitation, significantly eliminates the content of starch in Ginger Polysaccharide, the Ginger Polysaccharide dissolving of extraction
Good, the solution clear of property, hydroscopicity and moisturizing rate are respectively provided with compared with tremella polysaccharides and are obviously improved.
Applicant states that the present invention illustrates the method detailed of the present invention, but not office of the invention by above-described embodiment
It is limited to above-mentioned method detailed, that is, does not mean that the present invention has to rely on above-mentioned method detailed and could implemented.Art
Technical staff it will be clearly understood that any improvement in the present invention, equivalence replacement and auxiliary element to each raw material of product of the present invention
Addition, selection of concrete mode etc., within the scope of all falling within protection scope of the present invention and disclosing.
Claims (10)
1. a kind of extracting method of Ginger Polysaccharide, it is characterised in that comprise the following steps：
(1) low temperature extracts：Residue of the ginger after squeezing the juice is placed in refluxing extraction equipment, Extraction solvent is added, is carried
Take；
(2) thickening filtration：Extract solution is sieved, concentrated, filtered, purified, obtains refined filtration liquid；
(3) primary sedimentation：Ethanol is added into step (2) the refined filtration liquid and is centrifuged, precipitation is dried under reduced pressure；
(4) secondary precipitation：Ethanol is added in the supernatant obtained to step (3) centrifugation and is centrifuged, and precipitation is depressurized
Dry, the Ginger Polysaccharide is made.
2. extracting method according to claim 1, it is characterised in that step (1) residue and the Extraction solvent
Solid-liquid ratio is 1:(2-5), preferably 1:3；
Preferably, step (1) described Extraction solvent is purified water.
3. extracting method according to claim 1 or 2, it is characterised in that the temperature of step (1) described extraction is 50-90
DEG C, preferably 61 DEG C；
Preferably, the time of step (1) described extraction is 1-3h, preferably 2h；
Preferably, the number of step (1) described extraction is 1-3 times, preferably 2 times.
4. according to the extracting method described in claim 1-3, it is characterised in that the mesh number of step (2) described sieving is 100-300
Mesh, preferably 200 mesh；
Preferably, step (2) concentration uses reverse osmosis membrane；
Preferably, the pressure of step (2) described concentration is 5-30bar, preferably 20bar.
5. according to the extracting method described in claim any one of 1-4, it is characterised in that step (2) filtering uses film mistake
Filter system；
Preferably, the aperture of the membrane filtration system is 0.1-0.5 μm, preferably 0.22 μm；
Preferably, the pressure of the membrane filtration system is 1-5bar, preferably 3bar；
Preferably, step (2) purifying uses large aperture resin；
Preferably, the large aperture resin is D101 and/or AB-8 resin columns；
Preferably, the eluent of step (2) described purifying is ethanol water；
Preferably, the concentration of the ethanol water is 10-20%, preferably 15%.
6. according to the extracting method described in claim any one of 1-5, it is characterised in that the speed of step (3) described centrifugation is
3000-5000r/min, preferably 4000r/min；
Preferably, the time of step (3) described centrifugation is 5-20min, preferably 10min.
7. according to the extracting method described in claim any one of 1-6, it is characterised in that the speed of step (4) described centrifugation is
3000-5000r/min, preferably 4000r/min；
Preferably, the time of step (4) described centrifugation is 5-20min, preferably 10min.
8. according to the extracting method described in claim any one of 1-7, it is characterised in that comprise the following steps：
(1) low temperature extracts：Residue of the ginger after squeezing the juice is placed in refluxing extraction equipment, is 1 by solid-liquid ratio:(2-5) adds
Enter purified water, extracted 1-3 times at 50-90 DEG C, each 1-3h；
(2) thickening filtration：After extract solution is crossed into 100-300 mesh sieves, reverse osmosis concentration is carried out under 5-30bar pressure, in 1-
Filtered under 5bar using 0.1-0.5 μm of membrane filtration system, upper D101 and/or AB-8 resin columns are purified, and use 10-20%
Ethanol solution elutes, and obtains refined filtration liquid；
(3) primary sedimentation：It is 30-60% that ethanol to final volume concentration is added into step (2) the refined filtration liquid, in 3000-
5-20min is centrifuged under 5000r/min, precipitation is dried under reduced pressure；
(4) secondary precipitation：It is 70-95% to add ethanol to final volume concentration in the supernatant obtained to step (3) centrifugation,
5-20min is centrifuged under 3000-5000r/min, precipitation is dried under reduced pressure, the Ginger Polysaccharide is made.
9. a kind of extracting method as described in claim any one of 1-8 extracts obtained Ginger Polysaccharide.
10. a kind of Ginger Polysaccharide as claimed in claim 9 is used to prepare cosmetics.
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| CN106265329A (en) * | 2016-09-07 | 2017-01-04 | 山东省药学科学院 | A kind of conditioner and application thereof |
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| CN106726965A (en) * | 2016-12-26 | 2017-05-31 | 湖南御家化妆品制造有限公司 | Composite skin care product that antiallergic is releived and preparation method thereof |
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| CN106265329A (en) * | 2016-09-07 | 2017-01-04 | 山东省药学科学院 | A kind of conditioner and application thereof |
| CN106726965A (en) * | 2016-12-26 | 2017-05-31 | 湖南御家化妆品制造有限公司 | Composite skin care product that antiallergic is releived and preparation method thereof |
| CN106632719A (en) * | 2017-01-03 | 2017-05-10 | 南京农业大学 | Method for carrying out ultrasonic-assisted extraction on crude polysaccharide of fresh ginger residue |
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| CN113603800A (en) * | 2021-08-10 | 2021-11-05 | 澳门大学 | Ginger polysaccharide and preparation method and application thereof |
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