CN107692977B - OCT-based dual-mode optical micro-contrast imaging system - Google Patents
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- A—HUMAN NECESSITIES
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- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
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Abstract
An OCT-based dual mode optical micro-contrast imaging system, characterized by: the device comprises a light source, an indicating light, a circulator, a coupler, an adjustable reference arm, an adjustable sample arm, a spectrometer, an acquisition card and a computer; the invention has the advantages that: the dual-mode system can enable doctors to locate focus areas to scan more rapidly in clinical detection, save detection time, improve detection accuracy and greatly improve the practicability of an OCT-based optical micro-contrast imaging system.
Description
Technical Field
The present invention relates to an imaging system, and more particularly, to an OCT-based dual mode optical micro-contrast imaging system.
Background
Optical coherence tomography (Optical Coherence Tomography, OCT for short) is a novel biomedical optical imaging modality that can perform in-vivo, non-destructive imaging of a sample under test. Because of the advantages of high resolution, non-invasiveness, no radiation and the like, the method has wide application in a plurality of fields and has good development prospect. OCT can achieve micron-scale axial resolution (< 10 μm) providing high-precision depth images for in vivo subcellular bioassays. Another important feature of OCT technology is its highly scalable imaging depth, i.e., depth range. The most common commercial OCT systems typically range in depth from 2 to 4mm, most of which are used for retinal and endoscopic imaging.
In medicine, techniques for diagnosing certain diseases based on blood flow detection of human tissue are also playing an increasingly important role. Laser speckle blood flow imaging technology, doppler OCT blood flow detection technology, OCT-based angiography imaging. Wherein angiographic imaging constructs functional 3D angiographic or blood perfusion maps by measuring the variation of the amplitude, phase and complex values of the OCT signals. Angiographic imaging has been reported to achieve higher sensitivity in capillaries with slower flow rates. Angiographic methods have been successfully applied to human skin microcirculation vessel imaging. However, angiographic imaging has a small imaging range, typically only a few square millimeters. In clinical testing, however, a physician needs to perform multiple tests to determine the focal area. And if the range of the focus area is larger, multiple scanning and splicing are needed, so that the scanning accuracy is reduced, and the error of the scanning result is increased. There is a great need for a system that can detect vascular tissue over a large field of view and then quickly locate the focal area for scanning.
The invention provides a dual-mode optical micro-contrast imaging system based on OCT, which realizes a wide area mode by using a long focal length lens in a sample arm; a magnifying lens is used to achieve a high resolution mode. The computer controls the sample arm and the reference arm simultaneously, so that the two modes are switched rapidly.
For example, patent document 201510390567.9 discloses a method and a system for expanding OCT imaging depth by using a dual-spatial carrier frequency technology, in which two sets of scanning galvanometer systems are respectively arranged in one set of system to scan images with different depths, but the two sets of galvanometers greatly increase the cost of the system, and correspondingly require a light source with larger optical power, so that the cost is further increased. According to the invention, only the lens is replaced in the sample arm, and dual-mode imaging is realized for different scanning areas, so that the accuracy and the practicability of system scanning are greatly improved in medical diagnosis. In patent document 201620806921.1, an OCT reference arm automatic adjustment device, which is invented for facilitating the disassembly and adjustment, is adjusted by attaching a mirror to a console. The adjustable reference arm is arranged for realizing dual-mode imaging, and is completely controlled by a computer, so that the dual-mode imaging system is more convenient, accurate and quick.
Disclosure of Invention
The invention aims to provide a dual-mode optical micro-contrast imaging system based on OCT, which can solve the defects of the prior art, the imaging range of the current angiography imaging is smaller, usually only a few square millimeters, and in clinical detection, a doctor can determine a focus area through multiple detection; the dual-mode system can enable doctors to locate focus areas to scan more rapidly in clinical detection, save detection time, improve detection accuracy and greatly improve the practicability of an OCT-based optical micro-contrast imaging system.
The technical scheme of the invention is as follows: an OCT-based dual mode optical micro-contrast imaging system, characterized by: the device comprises a light source, an indicating light, a circulator, a coupler, an adjustable reference arm, an adjustable sample arm, a spectrometer, an acquisition card and a computer; the optical signals of the scanning laser emitted by the light source respectively enter the adjustable reference arm and the adjustable sample arm after passing through the circulator and the coupler, and light returned from the adjustable reference arm and the adjustable sample arm is interfered in the coupler to form coherent light; the returned coherent light is collected by a spectrometer through a circulator; the spectrometer collects optical signals and converts the optical signals into analog signals; the acquisition card acquires analog signals and transmits the analog signals to the computer; the computer performs signal processing, system imaging and system control; the adjustable sample arm comprises a long focal length lens for realizing a wide area mode and a magnifying lens for realizing a high resolution mode; the adjustable reference arm includes different optical path modes corresponding to a wide area mode and a high resolution mode of the adjustable sample arm, respectively; the adjustable reference arm and the adjustable sample arm are controlled by a computer and perform mode conversion; the indicating light enters the adjustable reference arm and the adjustable sample arm through the coupler respectively.
The center wavelength of the light source is 1310 nm, and the bandwidth is 60 nm.
The indicator light uses 660 nm red light to provide an indication of the scanning position.
The light signal emitted by the light source is divided into two paths through the circulator and the coupler, 10% of the light enters the adjustable reference arm, and 90% of the light enters the adjustable sample arm.
The long focal length lens and the magnifying lens of the adjustable sample arm are arranged on the electric rotating frame; the electric rotating frame is arranged on the electric bracket; the electric rotating frame is driven by an electric motor controlled by a computer; in the wide area mode, the rotating frame rotates to transmit scanning light to the center of the long-focal-length lens, and the lens is perpendicular to the light transmission direction; in the high resolution mode, the rotating frame rotates to transmit the scanning light to the central position of the magnifying lens, and the lens is perpendicular to the light transmission direction.
The focal length of the long-focal-length lens is 75mm, and the magnification of the magnifying lens is 10 times.
The adjustable reference arm comprises a collimator, a convex lens I, a plane reflecting mirror I corresponding to the convex lens I, a convex lens II, a plane reflecting mirror II corresponding to the convex lens II and an electric reflecting mirror baffle; the electric reflector baffle is driven by an electric motor controlled by a computer to change the reflection angle, light with different angles respectively enters the center position of the convex lens I or the convex lens II, and the lens is perpendicular to the light transmission direction.
When the adjustable sample arm is in a wide area mode, the electric reflector baffle is controlled by the electric motor through the computer to be horizontal and does not shade the light transmission of the reference arm, so that the light of the collimator is completely transmitted to the center of the convex lens I, and the convex lens I is perpendicular to the light transmission direction, thereby realizing the wide area imaging mode of the system; when the adjustable sample arm is in a high resolution mode, the electric reflector baffle forms an included angle of 45 degrees with the light transmission direction through the reflector baffle under the control of the motor by the computer, so that light emitted by the collimator is totally reflected to the center of the convex lens II through the reflector baffle, the convex lens II is perpendicular to the light transmission direction, and the marked area is scanned, so that the high resolution mode of the system is realized.
The adjustable reference arm comprises a collimator, a convex lens I, a plane reflecting mirror I corresponding to the convex lens I, a convex lens II, a plane reflecting mirror II corresponding to the convex lens II and an electric motor; the collimator is driven by an electric motor controlled by a computer to change the direction of emergent light, and the light in different directions respectively enters the center position of the convex lens I or the convex lens II, and the lens is perpendicular to the light transmission direction.
When the adjustable sample arm is in a wide area mode, the collimator rotates under the control of the electric motor, so that emergent light is transmitted to the center of the convex lens I, and the convex lens I is perpendicular to the light transmission direction, so that the wide area imaging mode of the system is realized; when the adjustable sample arm is in a high resolution mode, the collimator rotates under the control of the electric motor, so that emergent light is completely transmitted to the center of the convex lens II, the convex lens II is perpendicular to the light transmission direction, and the labeling area is scanned, so that the high resolution mode of the system is realized.
The adjustable reference arm comprises a collimator, a convex lens I, a plane reflecting mirror I corresponding to the convex lens I, an electric sliding support and a small fixed support; the convex lens I and the plane reflector I are fixedly arranged on the electric sliding support; the collimator is fixedly arranged with the small fixing bracket; the electric sliding support is driven by an electric motor controlled by a computer to change the distance between the convex lens I and the collimator and the distance between the plane reflecting mirror I are changed; the emergent light of the collimator enters the center position of the convex lens I, and the convex lens I is perpendicular to the light transmission direction.
When the adjustable sample arm is in a wide area mode, the electric sliding support moves in a direction away from the collimator under the control of the electric motor, so that the convex lens I and the plane reflector I are driven to reach corresponding positions, emergent light is transmitted to the center of the convex lens I, the convex lens I is perpendicular to the light transmission direction, and light returned by the sample arm and the reference arm is interfered, so that the wide area mode of the system is realized; when the adjustable sample arm is in a high resolution mode, the electric sliding support moves towards the direction of the collimator under the control of the electric motor, the convex lens I and the plane reflector I are driven to reach corresponding positions, emergent light is transmitted to the center of the convex lens I, the convex lens I is perpendicular to the light transmission direction, and the labeling area is scanned, so that the high resolution mode of the system is realized.
The adjustable reference arm comprises a collimator, a convex lens I, a plane reflecting mirror I corresponding to the convex lens I and an electrically adjustable optical retarder; the collimator is connected with an electrically adjustable optical delay, and different values are input into the electrically adjustable optical delay to control the optical path of the reference arm; the emergent light of the collimator enters the center position of the convex lens I, and the convex lens I is perpendicular to the light transmission direction.
When the adjustable sample arm is in a wide area mode, a fixed value A is input into the electric adjustable optical delay device, and the optical path of the reference arm is changed, so that the wide area mode of the system is realized; when the adjustable sample arm is in a high resolution mode, another fixed value B is input into the electrically adjustable optical delay device, the optical path of the reference arm is changed, and the labeling area is scanned, so that the high resolution mode of the system is realized.
The wide area mode has a scanning range of up to 750mm 2 (25 mm. Times.30 mm); in wide area mode, the system lateral resolution is 24.8 μm; in the high resolution mode, the lateral resolution of the system can be up to 6 μm.
The invention has the advantages that:
1. the invention provides a dual-mode optical micro-contrast imaging system based on OCT. The dual-mode system can enable doctors to locate focus areas to scan more rapidly in clinical detection, detection time is saved, detection accuracy is improved, and practicability of the OCT-based optical micro-contrast imaging system is greatly improved.
2. The long-focal-length lens is used in the sample arm, so that the scanning range of the system is enlarged, and a wide-area mode is realized; by using a magnifying lens in the sample arm, the resolution of the system imaging is increased, enabling a high resolution mode. And the optical path length of the sample arm changes as the focal length of the lens in the sample arm changes. In order to achieve coherent imaging of the return light of the sample arm and the reference arm, the optical path length of the sample arm needs to be adjusted accordingly. The invention controls the adjustable sample arm and the adjustable reference arm by a computer, so that the system can rapidly switch between two modes, and the practicability of the system in clinical detection is improved.
3. According to the invention, the computer is used for controlling the sample arm and the reference arm simultaneously, so that the two modes can be switched more rapidly. And the system uses visible light as indication light, so that the system can quickly and accurately position the region of interest in two modes.
Drawings
FIG. 1 is a block diagram of a dual mode optical micro-contrast imaging system based on OCT in accordance with the present invention;
fig. 2 is a schematic structural diagram of a dual-mode optical micro-contrast imaging system based on OCT according to a first embodiment of the present invention:
fig. 3 is a schematic structural diagram of a second embodiment of an OCT-based dual-mode optical micro-contrast imaging system according to the present invention:
fig. 4 is a schematic structural diagram of a dual-mode optical micro-contrast imaging system based on OCT according to a third embodiment of the present invention;
fig. 5 is a schematic structural diagram of a dual-mode optical micro-contrast imaging system based on OCT according to a fourth embodiment of the present invention.
Wherein 1 is a light source, 2 is an indicator light, 3 is a circulator, 4 is a coupler, 5 is an adjustable reference arm, 6 is an adjustable sample arm, 7 is a spectrometer, 8 is an acquisition card, 9 is a computer, 10 is a collimator, 11 is a convex lens I, 12 is a plane mirror I, 13 is a convex lens II, 14 is a plane mirror II, 15 is an electric reflector baffle, 16 is a long focal length lens, 17 is a magnifying lens, 18 is an electric rotating frame, 19 is an electric bracket, 20 is an electric motor, 21 is an electric sliding bracket, 22 is a small fixed bracket, and 23 is an electric adjustable light retarder.
Detailed Description
The present invention will be described in further detail with reference to specific examples. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
As shown in fig. 1, the present invention is a dual mode optical micro-contrast imaging system based on OCT. Comprising a light source 1; an indication light 2; a circulator 3; a coupler 4; an adjustable reference arm 5; an adjustable sample arm 6; a spectrometer 7; a pick-up card 8; a computer 9. Light source 1: the center wavelength is 1310 nm, the bandwidth is 60 nm, and the function is to emit scanning laser light. Indicator light 2: red light of 660 nm is typically used to provide an indication of the scanning position. Circulator 3: the returned coherent light is collected only by the spectrometer and is not returned to the light source. Coupler 4: light 10 emitted by the light source: 90 into the reference arm and the sample arm, respectively. Electrically adjustable reference arm 5: the reference arm optical path is electrically adjusted in a number of ways. Adjustable sample arm 6: the sample arm lens is switched electronically. Spectrometer 7: the optical signal is collected and converted to an analog signal. Acquisition card 8: analog signals are collected and transmitted to a computer. Computer 9: signal processing, system imaging and system control.
The optical signal from the light source 1 is split into two paths by the circulator 3 and the coupler 4, 10% of the light enters the adjustable reference arm 5 and 90% of the light enters the adjustable sample arm 6. Light returned from the adjustable sample arm 6 and the adjustable reference arm 5 interfere in the coupler 4 to form coherent light; the coherent light is collected by the spectrometer 7 through the circulator and converted into an analog signal; the analog signals are collected by the collection card 8 and transmitted to the computer 9; the computer demodulates and images the signals. Using a long focal length lens 16 in the adjustable sample arm 6, the system scan range becomes large, enabling a wide area mode; the use of a magnifying lens 17 in the adjustable sample arm 6 increases the resolution of the system imaging, enabling a high resolution mode. While the optical path length of the adjustable sample arm 6 changes as the focal length of the lens in the adjustable sample arm 6 changes. In order to achieve coherent imaging of the return light of the adjustable sample arm 6 and the adjustable reference arm 5, the optical path length of the adjustable reference arm 5 needs to be adjusted accordingly. The invention controls the adjustable sample arm 6 and the adjustable reference arm 5 by a computer, so that the system can rapidly switch between two modes, and the practicability of the system in clinical detection is improved.
Embodiment one: as shown in fig. 2, two different optical paths of the adjustable reference arm 5 are realized with a motorized mirror baffle 15. The adjustable reference arm 5 comprises a collimator 10, a convex lens I11, a plane reflecting mirror I12 corresponding to the convex lens I11, a convex lens II 13, a plane reflecting mirror II 14 corresponding to the convex lens II 13 and an electric reflecting mirror baffle 15. The adjustable sample arm 6 comprises: a long focal length lens 16 with a focal length of 75mm, an amplifying lens 17 with a magnification x 10, an electric rotating frame 18, and an electric bracket 19. The adjustable sample arm 6 and the adjustable reference arm 5 are each connected to an electric motor controlled by a computer 9, the control adjustment being performed by means of the computer 9.
First, when the wide area mode is entered, the rotating frame 18 in the sample arm 6 can be adjusted to rotate clockwise, so that the scanning light is transmitted to the center position of the long focal length lens 16, and the long focal length lens 16 is perpendicular to the light transmission direction. At the same time, the electric mirror baffle 15 in the adjustable reference arm 5 is horizontal under the control of the electric motor by the computer 9 and does not block the light transmission of the adjustable reference arm 5, so that the light of the collimator 10 is totally transmitted into the convex lens I11. Thus realizing the wide area imaging mode of the system, and the maximum scanning range can reach 750mm 2 (25 mm. Times.30 mm). Then, the region of interest is marked with a mouse, and the high resolution mode is entered, and the rotating frame 18 is rotated counterclockwise, so that the scanning light is transmitted to the center position of the magnifying lens 17, and the magnifying lens 17 is perpendicular to the light transmission direction. Meanwhile, in the reference arm, under the control of the motor, the electric reflector baffle 15 forms an included angle of 45 degrees with the light transmission direction, so that the light emitted by the collimator is totally reflected to the convex lens II through the electric reflector baffle 1513, the scanning range is the mouse marking range, so as to realize the high resolution mode of the system. In wide area mode, the system lateral resolution is 24.8 μm; while in the high resolution mode the lateral resolution of the system may be up to 6 μm.
Embodiment two: as shown in fig. 3, two different optical paths of the reference arm are realized by changing the direction of the light exiting the collimator 10. The adjustable reference arm 5 comprises a collimator 10, a convex lens I11, a plane mirror I12 corresponding to the convex lens I11, a convex lens II 13, a plane mirror II 14 corresponding to the convex lens II 13 and an electric motor 20. The adjustable sample arm 6 comprises: a long focal length lens 16 with a focal length of 75mm, an amplifying lens 17 with a magnification x 10, an electric rotating frame 18, and an electric bracket 19. The adjustable sample arm 6 and the adjustable reference arm 5 are each connected to an electric motor controlled by a computer 9, the control adjustment being likewise performed by means of the computer 9.
First, when the wide area mode is entered, the rotating frame 18 in the sample arm is rotated clockwise, so that the scanning light is transmitted to the center position of the long focal length lens 16, and the long focal length lens 16 is perpendicular to the light transmission direction. At the same time, the collimator 10 rotates under the control of the electric motor 20 so that the outgoing light is transmitted to the center of the convex lens i 11, and the convex lens i 11 is perpendicular to the light transmission direction. Thereby implementing a wide area imaging mode of the system. Then, the region of interest is marked with a mouse, and the high resolution mode is entered, and the rotating frame 18 is rotated counterclockwise, so that the scanning light is transmitted to the center position of the magnifying lens 17, and the magnifying lens 17 is perpendicular to the light transmission direction. Meanwhile, the electric motor 20 controls the collimator 10 to rotate, so that emergent light is completely transmitted to the center of the convex lens II 13, and the convex lens II 13 is perpendicular to the light transmission direction. The scanning range is a mouse labeling range, so that a high-resolution mode of the system is realized. In wide area mode, the system lateral resolution is 24.8 μm; while in the high resolution mode the lateral resolution of the system may be up to 6 μm.
Embodiment III: as shown in fig. 4, the different optical paths of the adjustable reference arm 5 are realized with an electrically powered sliding support 21. The adjustable reference arm 5 comprises a collimator 10, a convex lens I11, a plane reflecting mirror I12 corresponding to the convex lens I11, an electric sliding support 21 and a small fixed support 22. The adjustable sample arm 6 comprises: a long focal length lens 16 with a focal length of 75mm, a magnification x 10 magnifying lens 17. The adjustable sample arm 6 and the adjustable reference arm 5 are each connected to an electric motor controlled by a computer 9, the control adjustment being likewise performed by means of the computer 9.
First, when the wide area mode is entered, the rotating frame 18 in the sample arm 6 can be adjusted to rotate clockwise, so that the scanning light is transmitted to the center position of the long focal length lens 16, and the long focal length lens 16 is perpendicular to the light transmission direction. At the same time, the collimator 10 is fixed by the small fixed bracket 22, the electric motor controls the electric sliding bracket 21 to drive the convex lens 11 and the plane mirror 12 to reach the corresponding positions, so that the light returned by the adjustable sample arm 6 and the adjustable reference arm 5 is interfered, and the wide area mode of the system is realized. Then, the region of interest is marked with a mouse, and the high resolution mode is entered, and the rotating frame 18 is rotated counterclockwise, so that the scanning light is transmitted to the center position of the magnifying lens 17, and the magnifying lens 17 is perpendicular to the light transmission direction. At the same time, the electric motor controls the electric sliding support 21 to drive the convex lens 11 and the plane mirror 12 to move in parallel to the direction of the collimator to reach a proper position. And then scanning the mouse labeling area, thereby realizing a high-resolution mode of the system.
The optical path of the adjustable reference arm 5 is flexibly and variously adjusted, and long-focus lenses with different focuses and magnifying lenses with different magnifications can be used on the adjustable sample arm 6, so that the imaging range and the imaging resolution are more flexible and changeable.
Embodiment four: as shown in fig. 5, the different optical paths of the adjustable reference arm 5 are realized with an electrically adjustable optical retarder 23. The adjustable reference arm 5 comprises a collimator 10, a convex lens I11, a plane mirror I12 corresponding to the convex lens I11 and an electrically adjustable optical retarder 23. The adjustable sample arm 6 comprises: a long focal length lens 16 with a focal length of 75mm, a magnification x 10 magnifying lens 17. The computer 9 controls the lens switching of the adjustable sample arm 6 by means of an electric motor. Different values are input in the electrically adjustable optical retarder 23 to control the optical path length of the adjustable reference arm 5.
First, when the wide area mode is entered, the rotating frame 18 in the sample arm 6 can be adjusted to rotate clockwise, so that the scanning light is transmitted to the center position of the long focal length lens 16, and the long focal length lens 16 is perpendicular to the light transmission direction. At the same time, a fixed value A is input into the electrically adjustable optical delayer 23, and the optical path of the adjustable reference arm 5 is changed, so that the wide area mode of the system is realized. Then, the region of interest is marked with a mouse, and the high resolution mode is entered, and the rotating frame 18 is rotated counterclockwise, so that the scanning light is transmitted to the center position of the magnifying lens 17, and the magnifying lens 17 is perpendicular to the light transmission direction. At the same time, another fixed value B is input into the electrically adjustable optical retarder 23, changing the optical path of the adjustable reference arm 5. And then scanning the mouse labeling area, thereby realizing a high-resolution mode of the system.
The different values of the input in the electrically adjustable optical retarder 23 change the optical path length of the adjustable reference arm 5, and a long focal length lens with different focal lengths and a magnifying lens with different magnification can be used in the adjustable sample arm 6, so that the imaging range and the imaging resolution which can be achieved by the system are more changeable.
Claims (9)
1. An OCT-based dual mode optical micro-contrast imaging system, characterized by: the device comprises a light source, an indicating light, a circulator, a coupler, an adjustable reference arm, an adjustable sample arm, a spectrometer, an acquisition card and a computer; the optical signals of the scanning laser emitted by the light source respectively enter the adjustable reference arm and the adjustable sample arm after passing through the circulator and the coupler, and light returned from the adjustable reference arm and the adjustable sample arm is interfered in the coupler to form coherent light; the returned coherent light is collected by a spectrometer through a circulator; the spectrometer collects optical signals and converts the optical signals into analog signals; the acquisition card acquires analog signals and transmits the analog signals to the computer; the computer performs signal processing, system imaging and system control; the adjustable sample arm comprises a long focal length lens for realizing a wide area mode and a magnifying lens for realizing a high resolution mode; the adjustable reference arm includes different optical path modes corresponding to a wide area mode and a high resolution mode of the adjustable sample arm, respectively; the adjustable reference arm and the adjustable sample arm are controlled by a computer and perform mode conversion; the indicating light respectively enters the adjustable reference arm and the adjustable sample arm through the coupler; the long focal length lens and the magnifying lens of the adjustable sample arm are arranged on the electric rotating frame; the electric rotating frame is arranged on the electric bracket; the electric rotating frame is driven by an electric motor controlled by a computer; in the wide area mode, the rotating frame rotates to transmit scanning light to the center of the long-focal-length lens, and the lens is perpendicular to the light transmission direction; in the high resolution mode, the rotating frame rotates to transmit the scanning light to the central position of the magnifying lens, and the lens is perpendicular to the light transmission direction.
2. The OCT-based dual-mode optical micro-contrast imaging system of claim 1, wherein: the adjustable reference arm comprises a collimator, a convex lens I, a plane reflecting mirror I corresponding to the convex lens I, a convex lens II, a plane reflecting mirror II corresponding to the convex lens II and an electric reflecting mirror baffle; the electric reflector baffle is driven by an electric motor controlled by a computer to change the reflection angle, light with different angles respectively enters the center position of the convex lens I or the convex lens II, and the lens is perpendicular to the light transmission direction.
3. The OCT-based dual-mode optical micro-contrast imaging system of claim 2, wherein: when the adjustable sample arm is in a wide area mode, the electric reflector baffle is controlled by the electric motor through the computer to be horizontal and does not shade the light transmission of the reference arm, so that the light of the collimator is completely transmitted to the center of the convex lens I, and the convex lens I is perpendicular to the light transmission direction, thereby realizing the wide area imaging mode of the system; when the adjustable sample arm is in a high resolution mode, the electric reflector baffle forms an included angle of 45 degrees with the light transmission direction through the reflector baffle under the control of the motor by the computer, so that light emitted by the collimator is totally reflected to the center of the convex lens II through the reflector baffle, the convex lens II is perpendicular to the light transmission direction, and the marked area is scanned, so that the high resolution mode of the system is realized.
4. The OCT-based dual-mode optical micro-contrast imaging system of claim 1, wherein: the adjustable reference arm comprises a collimator, a convex lens I, a plane reflecting mirror I corresponding to the convex lens I, a convex lens II, a plane reflecting mirror II corresponding to the convex lens II and an electric motor; the collimator is driven by an electric motor controlled by a computer to change the direction of emergent light, and the light in different directions respectively enters the center position of the convex lens I or the convex lens II, and the lens is perpendicular to the light transmission direction.
5. The OCT-based dual-mode optical micro-contrast imaging system of claim 4, wherein: when the adjustable sample arm is in a wide area mode, the collimator rotates under the control of the electric motor, so that emergent light is transmitted to the center of the convex lens I, and the convex lens I is perpendicular to the light transmission direction, so that the wide area imaging mode of the system is realized; when the adjustable sample arm is in a high resolution mode, the collimator rotates under the control of the electric motor, so that emergent light is completely transmitted to the center of the convex lens II, the convex lens II is perpendicular to the light transmission direction, and the labeling area is scanned, so that the high resolution mode of the system is realized.
6. The OCT-based dual-mode optical micro-contrast imaging system of claim 1, wherein: the adjustable reference arm comprises a collimator, a convex lens I, a plane reflecting mirror I corresponding to the convex lens I, an electric sliding support and a small fixed support; the convex lens I and the plane reflector I are fixedly arranged on the electric sliding support; the collimator is fixedly arranged with the small fixing bracket; the electric sliding support is driven by an electric motor controlled by a computer to change the distance between the convex lens I and the collimator and the distance between the plane reflecting mirror I are changed; the emergent light of the collimator enters the center position of the convex lens I, and the convex lens I is perpendicular to the light transmission direction.
7. The OCT-based dual-mode optical micro-contrast imaging system of claim 6, wherein: when the adjustable sample arm is in a wide area mode, the electric sliding support moves in a direction away from the collimator under the control of the electric motor, so that the convex lens I and the plane reflector I are driven to reach corresponding positions, emergent light is transmitted to the center of the convex lens I, the convex lens I is perpendicular to the light transmission direction, and light returned by the sample arm and the reference arm is interfered, so that the wide area mode of the system is realized; when the adjustable sample arm is in a high resolution mode, the electric sliding support moves towards the direction of the collimator under the control of the electric motor, the convex lens I and the plane reflector I are driven to reach corresponding positions, emergent light is transmitted to the center of the convex lens I, the convex lens I is perpendicular to the light transmission direction, and the labeling area is scanned, so that the high resolution mode of the system is realized.
8. The OCT-based dual-mode optical micro-contrast imaging system of claim 1, wherein: the adjustable reference arm comprises a collimator, a convex lens I, a plane reflecting mirror I corresponding to the convex lens I and an electrically adjustable optical retarder; the collimator is connected with an electrically adjustable optical delay, and different values are input into the electrically adjustable optical delay to control the optical path of the reference arm; the emergent light of the collimator enters the center position of the convex lens I, and the convex lens I is perpendicular to the light transmission direction.
9. The OCT-based dual-mode optical micro-contrast imaging system of claim 8, wherein: when the adjustable sample arm is in a wide area mode, a fixed value is input into the electric adjustable optical delay device, and the optical path of the reference arm is changed, so that the wide area mode of the system is realized; when the adjustable sample arm is in a high-resolution mode, another fixed value is input into the electrically adjustable optical delay device, the optical path of the reference arm is changed, and the labeling area is scanned, so that the high-resolution mode of the system is realized.
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