CN107683335A - 用于核酸分离的自动化方法 - Google Patents

用于核酸分离的自动化方法 Download PDF

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Publication number
CN107683335A
CN107683335A CN201680030749.8A CN201680030749A CN107683335A CN 107683335 A CN107683335 A CN 107683335A CN 201680030749 A CN201680030749 A CN 201680030749A CN 107683335 A CN107683335 A CN 107683335A
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CN
China
Prior art keywords
nucleic acid
alcohol
separation
butanol
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201680030749.8A
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English (en)
Chinese (zh)
Inventor
海尔特·梅尔斯曼
克拉斯·德卡尼埃尔
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Biocartis SA
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Biocartis SA
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Application filed by Biocartis SA filed Critical Biocartis SA
Publication of CN107683335A publication Critical patent/CN107683335A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201680030749.8A 2015-06-09 2016-06-09 用于核酸分离的自动化方法 Pending CN107683335A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15171261.9 2015-06-09
EP15171261 2015-06-09
PCT/EP2016/063150 WO2016198519A1 (en) 2015-06-09 2016-06-09 Automatable method for nucleic acid isolation

Publications (1)

Publication Number Publication Date
CN107683335A true CN107683335A (zh) 2018-02-09

Family

ID=53373331

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680030749.8A Pending CN107683335A (zh) 2015-06-09 2016-06-09 用于核酸分离的自动化方法

Country Status (7)

Country Link
US (1) US10829758B2 (https=)
EP (2) EP3307907B1 (https=)
JP (1) JP6815334B2 (https=)
CN (1) CN107683335A (https=)
CA (1) CA2982706A1 (https=)
ES (1) ES2965460T3 (https=)
WO (1) WO2016198519A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114450419A (zh) * 2019-08-08 2022-05-06 拜奥卡蒂斯生物股份有限公司 新型核酸纯化化学物质

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108138366A (zh) 2015-07-23 2018-06-08 比奥卡尔齐斯股份有限公司 优化的临床样品测序
EP4303311A3 (en) * 2017-09-18 2024-04-03 Phase Scientific International, Ltd. Method for using aqueous two-phase system for the isolation, purification and/or concentration of short nucleic acid fragments
EP3884066A2 (en) * 2018-11-19 2021-09-29 Biocartis NV Enhanced detection of low-copy-number nucleic acids in an integrated workflow
WO2021130415A1 (en) * 2019-12-23 2021-07-01 Biopsense Oy Method, automated system and cartridge for extraction of cell-free nucleic acids from a blood sample
EP4684011A1 (en) * 2023-03-20 2026-01-28 Bioecho Life Sciences GmbH Method for isolating nucleic acid from a biological sample

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004108925A1 (en) * 2003-06-04 2004-12-16 Qiagen As Method for sequentially isolating dna and rna from the same nucleic acid-containing sample
CN102046793A (zh) * 2008-05-30 2011-05-04 恰根有限公司 用于分离和/或纯化核酸的裂解、结合和/或洗涤试剂
EP2345719A1 (en) * 2010-01-18 2011-07-20 Qiagen GmbH Method for isolating small RNA
WO2013024072A1 (en) * 2011-08-12 2013-02-21 Qiagen Gmbh Method for isolating nucleic acids
WO2014144174A1 (en) * 2013-03-15 2014-09-18 Abbott Molecular Inc. Compositions and methods for nucleic acid extraction

Family Cites Families (12)

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Publication number Priority date Publication date Assignee Title
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
DE19746874A1 (de) 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
GB2346615B (en) * 1998-11-17 2003-10-15 Cambridge Molecular Tech Isolating nucleic acid
DE60137203D1 (de) * 2000-03-24 2009-02-12 Qiagen Gmbh Poröse ferro- oder ferrimagnetische glasteilchen für molekültrennung
JP2005137298A (ja) * 2003-11-07 2005-06-02 Fuji Photo Film Co Ltd 核酸の分離精製方法
EP1690938A1 (de) * 2005-02-11 2006-08-16 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden
US8703476B2 (en) 2005-06-30 2014-04-22 Biocartis Sa Cartridge for automated medical diagnostics
JP2007325562A (ja) * 2006-06-09 2007-12-20 Fujifilm Corp 核酸抽出法
EP2128169A1 (de) 2008-05-30 2009-12-02 Qiagen GmbH Verfahren zur Isolierung von kurzkettigen Nukleinsäuren
EP2264168B1 (de) * 2009-06-18 2014-12-17 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren
EP2770056B1 (en) * 2012-07-18 2017-05-31 Zymo Research Corporation Nucleic acid purification

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004108925A1 (en) * 2003-06-04 2004-12-16 Qiagen As Method for sequentially isolating dna and rna from the same nucleic acid-containing sample
CN102046793A (zh) * 2008-05-30 2011-05-04 恰根有限公司 用于分离和/或纯化核酸的裂解、结合和/或洗涤试剂
EP2345719A1 (en) * 2010-01-18 2011-07-20 Qiagen GmbH Method for isolating small RNA
WO2013024072A1 (en) * 2011-08-12 2013-02-21 Qiagen Gmbh Method for isolating nucleic acids
WO2014144174A1 (en) * 2013-03-15 2014-09-18 Abbott Molecular Inc. Compositions and methods for nucleic acid extraction

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114450419A (zh) * 2019-08-08 2022-05-06 拜奥卡蒂斯生物股份有限公司 新型核酸纯化化学物质

Also Published As

Publication number Publication date
EP3307907C0 (en) 2023-11-15
EP3307907B1 (en) 2023-11-15
EP3307907A1 (en) 2018-04-18
WO2016198519A1 (en) 2016-12-15
US20180291367A1 (en) 2018-10-11
JP6815334B2 (ja) 2021-01-20
JP2018518171A (ja) 2018-07-12
US10829758B2 (en) 2020-11-10
EP4186980A1 (en) 2023-05-31
ES2965460T3 (es) 2024-04-15
CA2982706A1 (en) 2016-12-15

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Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20180209

Assignee: Guangzhou Wanfu catis Biotechnology Co., Ltd

Assignor: Wanfu Cadiz Co., Ltd

Contract record no.: X2020990000658

Denomination of invention: Automated methods for nucleic acid separation

License type: Common License

Record date: 20201203

EE01 Entry into force of recordation of patent licensing contract
CB02 Change of applicant information

Address after: Mechelen

Applicant after: Baiocatis biology Co.,Ltd.

Address before: Mechelen

Applicant before: BIOCARTIS N.V.

CB02 Change of applicant information