CN107669529A - A kind of ginkgo extract - Google Patents

A kind of ginkgo extract Download PDF

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Publication number
CN107669529A
CN107669529A CN201710980981.4A CN201710980981A CN107669529A CN 107669529 A CN107669529 A CN 107669529A CN 201710980981 A CN201710980981 A CN 201710980981A CN 107669529 A CN107669529 A CN 107669529A
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China
Prior art keywords
weight
ginkgo
extract
ginkgo extract
acid
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CN201710980981.4A
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Inventor
曹祥
汤继纯
马巍
张俭永
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Jiangsu Kanion Yousaijin Biotechnology Co Ltd
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Jiangsu Kanion Yousaijin Biotechnology Co Ltd
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Priority to CN201710980981.4A priority Critical patent/CN107669529A/en
Publication of CN107669529A publication Critical patent/CN107669529A/en
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005Antimicrobial preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/02Preparations for care of the skin for chemically bleaching or whitening the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Dermatology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a kind of ginkgo extract, the extract solution includes:(a) flavone compound of 30~50% weight;(b) ginkgolides of 5~10% weight;(c) proanthocyanidin of 5~15% weight;(d) ginkgoic acid of 3~10% weight;(e) it is less than the polypenthylene alcohols ester of 3% weight;And (f) is more than the alkyl phenolic acid compound of 5% weight.The present invention has whitening effect; contained flavone component can hinder formation and calmness of the pigment in skin corium in the present invention; have the function that skin whitening and preventing and treating pigment patch; the flavonol contained is the street cleaner of free radical, can protect corium confluent monolayer cells, improves blood circulation; prevent cell is oxidized from producing wrinkle; level of lipid is reduced, reduces freckle, skin of moistening.The present invention also has sterilization and bacteriostasis effect simultaneously.

Description

A kind of ginkgo extract
Technical field
The present invention relates to a kind of ginkgo extract.
Background technology
Ginkgoaceae plant Ginkgo biloba (Ginkgo biloba) is the endemic tree in China, belongs to deciduous tree.Ginkgo appears in It is plant most ancient in the gymnosperm that carries over after quaternary glacier motion before several hundred million years, the alive ginkgo of existing work Rare and disperse, upper veteran at the age of one hundred years old has been mended common, and it has all become extinct with the other plant of guiding principle, so ginkgo has activation again The laudatory title of stone.Mutation and kind have:Yellow leaf ginkgo, tower-like ginkgo, baiera.26 kinds of weeping branch ginkgo, variegated leaf ginkgo etc..
Ginkgo leaf has extensive bioactivity, containing a variety of chemical compositions, mainly including flavonoids, terpene, polysaccharide, phenol Class.Organic acid.Alkaloid.Amino acid, trace element etc..Wherein, vitamin C, vitamin E, carrotene and calcium, phosphorus, boron, The Mineral Concentrations such as selenium also very abundant.Flavones ingredient in ginkgo biloba extract has very strong pharmacological activity, main tool There are following several effects:1st, free radical is eliminated, and the cell destroyed to free radical has certain repair;2nd, brain can be increased Vascular flow, cerebral vascular resistance is reduced, improve Cerebrovascular disorders;3rd, protect brain cell from ischemic damage;4th, blood vessel is adjusted Power, strengthen immunity of organisms, to coronary heart diseases and angina pectoris.The diseases such as the cerebrovascular have the effect of certain.At present, China, the U.S., Europe Ginkgo agent is classified as medicine by alliance etc., and other many countries are also classified as health food or over-the-counter medications.
For the healthy property for knowing very well ginkgo, although existing ginkgo biloba extract has met market in terms of medical value Purposes, but now it is desirable to improve or in addition performance product.Especially pursuit of the modern female to U.S., people Wish that develop a kind of ginkgo biloba extract of new type natural plays a role in cosmetic fields such as skin cares, and contained by ginkgo leaf Flavone component can hinder formation and calmness of the pigment in skin corium, have the function that skin whitening and subtract light color element patch, this Outside, the trace element such as the manganese in ginkgo leaf, molybdenum, also can scavenging activated oxygen, therefore ginkgo biloba extract is in the valency of cosmetic field Value has theoretical foundation, and ginkgo biloba extract of the prior art seldom pays close attention to its application value in cosmetic field.Therefore, The present invention is based on above mentioned problem, there is provided a kind of ginkgo biloba extract for not adding active component.
The content of the invention
The technical problem to be solved in the present invention is, for the drawbacks described above of prior art, there is provided a kind of ginkgo extract, The ginkgo extract has skin whitening, prevents the characteristics such as pigment patch and smoothing wrinkle.
In order to realize above-mentioned technical purpose, reach above-mentioned technique effect, technical scheme provided by the invention is:A kind of ginkgo Extract solution, the extract solution include:
(a) flavone compound of 30~50% weight;
(b) ginkgolides of 5~10% weight;
(c) proanthocyanidin of 5~15% weight;
(d) ginkgoic acid of 3~10% weight;
(e) it is less than the polypenthylene alcohols ester of 3% weight;And
(f) it is more than the alkyl phenolic acid compound of 5% weight.
Further, the flavone compound includes glycosides displayed and flavonols.
Further, the ginkgolides includes ginkalide A, ginkolide B, ginkalide C, ginkgo lactones M, ginkgo Lactones J, wherein content of the bilobalide J in ginkgo extract is not less than 4%.
Research shows, when endocrine disturbance or the imbalance of people, internal foster free radical will be excessive, but now aoxidizes Thing mutase deficiency, causes excessive oxygen radical to produce oxidation reaction with corium confluent monolayer cells, forms substantial amounts of lipofuscin, cause Chloasma or cyasma.The present invention can hinder formation of the pigment in skin corium containing more flavone compound, reach beautiful The effect of white muscle skin and preventing and treating pigment patch.When the skin of human body updates completely simultaneously, the new life that skin corium is formed is thin Born of the same parents, just by excessive free-radical oxidation, already belong to old when not arriving at also to skin surface when it is arrived to epidermis Change cell.The glycosides displayed and flavonols that contain in the present invention are all the street cleaners of free radical, can protect corium confluent monolayer cells, are improved Blood circulation, prevent cell is oxidized from producing wrinkle, there is the effect of crease-resistant.
Further, the proanthocyanidin containing 8% weight in the ginkgo extract.
Further, the ginkgoic acid containing 4% weight in the ginkgo extract.
Further, the alkyl phenolic acid compound includes protocatechuic acid, vanillic acid, caffeic acid, p- coumaric acids, asafoetide Acid and chlorogenic acid.
It is another object of the present invention to provide the method for preparing ginkgo extract, this method is made up of the following steps:
Step 1:Ginkgo leaf or dry grinding is taken to mix powder with solid-phase reagent to powder, add polar solvent immersion, add Heat backflow produces the first extract solution.
Step 2:First extract solution is diluted with water, and adjusts pH value 4~6, filtering, and filtrate is added on processed good macroreticular resin It is finely divided on post, first rinses resin column with purified water, then rinse resin column with sodium acetate solution, then with low concentration to height Concentration ethanol carries out gradient washes resin column, collects eluent, after miillpore filter refined filtration, obtains the second extract solution.
Step 3:Second extract solution is concentrated, and adding high concentration ethanol makes alcohol content reach 90%, with 20% hydroxide Sodium adjusts pH value, stands, and supernatant also use filtering with microporous membrane, recovery second through miillpore filter refined filtration after precipitation centrifuge Alcohol adds purified water to dilute, filled in container, carry out high-temperature sterilization, obtain ginkgo extract to without alcohol taste.
More specifically, according to the ginkgo extract that obtains of the present invention, generally show flavonoid content be 30~ 50%, more preferably 37~45%, it is preferably 40~45%.
Usual ginkgo extract of the present invention, contains:
(i) it is less than the polysaccharide compound of 7% weight;
(ii) it is higher than 150ppm biflavone;
(iii) terpene lactones of 10~25% weight.
Contain manganese and molybdenum trace element in the ginkgo extract.
In the ginkgo extract of the present invention:
1st, the present invention has whitening effect, and contained flavone component can hinder pigment in skin corium in of the invention Formed and calm, have the function that skin whitening and preventing and treating pigment patch,
2nd, the present invention has the effect of smoothing wrinkle, and the flavonol contained in the present invention is the street cleaner of free radical, can be protected Corium confluent monolayer cells, improve blood circulation, prevent cell is oxidized from producing wrinkle, reduce level of lipid, reduce freckle, profit Damp skin.The trace element such as manganese and molybdenum simultaneously, it can also remove oxyradical and suppress melanin growth.
3rd, the present invention also has a sterilization and bacteriostasis effect, and the bilobalide J of the invention contained has stronger sterilization and antibacterial Effect.
Embodiment
For the clearer explanation present invention, with reference to embodiment, the present invention will be further described.
The present invention provides a kind of ginkgo extract, and the extract solution includes:
(a) flavone compound of 30~50% weight;
(b) ginkgolides of 5~10% weight;
(c) proanthocyanidin of 5~15% weight;
(d) ginkgoic acid of 3~10% weight;
(e) it is less than the polypenthylene alcohols ester of 3% weight;
(f) it is more than the alkyl phenolic acid compound of 5% weight;
Preferably, flavone compound includes glycosides displayed and flavonols.
Preferably, ginkgolides includes ginkalide A, ginkolide B, ginkalide C, ginkgo lactones M, ginkgo lactones J, Content of the wherein described bilobalide J in ginkgo extract is not less than 4%.
Prepare embodiment 1
The preparation method of ginkgo extract of the present invention, this method are made up of the following steps:
Step 1:Ginkgo leaf or dry grinding is taken to mix powder with solid-phase reagent sodium carbonate or sodium acid carbonate to powder, add 60% alcohol solvent for entering 3 times of powder quality soaks more than 10 hours, and heating and refluxing extraction at least 1 time, each at least one is small When, filtering, merge extract solution, obtain the first extract solution.
Step 2:First extract solution is diluted with water to 3 times of amount volumes, and regulation pH value to 3.5, filtering, filtrate, which is added on, have been located In the macroporous resin column managed, first adsorbed with slower flow velocity, then rinse resin column, Ran Houyong with 2 times of amount purified waters of resin 10% sodium acetate solution of 3 times of amounts of amount of resin rinses resin column, then enters to rinse resin column with 1 times of 20% ethanol of amount of resin, Finally with 60% alcohol flushing resin post of 3 times of amounts of resin, eluent is collected, after 0.2um miillpore filter refined filtration, reclaims second Alcohol, obtain the second extract solution.
Step 3:Second extract solution is concentrated, adding high concentration ethanol makes alcohol content reach 90%, with 20% hydrogen-oxygen Change sodium adjusts pH value 8.0, stands more than 12 hours, miillpore filter refined filtration of the supernatant through 0.2um, after precipitation centrifuge With 0.2um filtering with microporous membrane, recovery ethanol adds 1 times of amount purified water dilution, filled in suitable container, 120 to without alcohol taste DEG C of 1 hour carries out high-temperature sterilization, and ginkgo extract is obtained after cooling.
After measured, flavonoid content is 41.8% in the extract solution, and the content of proanthocyanidin is 10%, in ginkgo Ester A content is 2.1%, the content of ginkolide B is 1.5%, the content of ginkalide C is 1.8%, ginkgo lactones M contains It is 5.6% to measure as the content of 0.9%, ginkgo lactones J.
Contain in the ginkgo extract of usual the present embodiment 1:The polysaccharide compound of 7% weight;180ppm biflavone; The terpene lactones of 14% weight.
Also containing manganese and molybdenum trace element in the ginkgo extract obtained in this preparation embodiment 1.
Prepare embodiment 2
Step 1:Ginkgo leaf or dry grinding is taken to mix powder with solid-phase reagent aluminium hydroxide to powder, add powder matter 5 times of 80% alcohol solvent of amount soaks more than 12 hours, heating and refluxing extraction 2 hours, second of addition, 2 times of 80% ethanol of amount, adds Circumfluence distillation 1 hour, filtering, merge extract solution, obtain the first extract solution.
Step 2:First extract solution is diluted with water to 5 times of amount volumes, and regulation pH value to 4.2, filtering, filtrate, which is added on, have been located In the macroporous resin column managed, first adsorbed with slower flow velocity, then rinse resin column, Ran Houyong with 3 times of amount purified waters of resin 5% sodium acetate solution of 5 times of amounts of amount of resin rinses resin column, then enters to rinse resin column with 3 times of 20% ethanol of amount of resin, most Afterwards with 80% alcohol flushing resin post of 3 times of amounts of resin, eluent is collected, after 0.2um miillpore filter refined filtration, reclaims second Alcohol, obtain the second extract solution.
Step 3:Second extract solution is concentrated, adding high concentration ethanol makes alcohol content reach 90%, with 10% hydrogen-oxygen Change sodium and adjust pH value 7.8, stand 24 hours, miillpore filter refined filtration of the supernatant through 0.2um, precipitation is also used with after centrifuge 0.2um filtering with microporous membrane, recovery ethanol add 0.5 times of amount purified water dilution, filled in suitable container, 120 to without alcohol taste High-temperature sterilization is carried out DEG C 3 hours, ginkgo extract is obtained after cooling.
After measured, the ginkgo extract includes:
(a) flavone compound of 46.3% weight;
(b) ginkgolides of 8% weight;
(c) proanthocyanidin of 14% weight;
(d) ginkgoic acid of 8% weight;
(e) the polypenthylene alcohols ester of 2% weight;
(f) the alkyl phenolic acid compound of 12% weight;
Abovementioned alkyl phenolic acid compound includes protocatechuic acid, vanillic acid, caffeic acid, p- coumaric acids, forulic acid and green Ortho acid.
Contain in the ginkgo extract of this preparation embodiment 2:The polysaccharide compound of 5% weight;250ppm biflavone; The terpene lactones of 15% weight.
Also containing manganese and molybdenum trace element in the ginkgo extract obtained in this preparation embodiment 2.
Prepare embodiment 3
Step 1:Ginkgo leaf or dry grinding is taken to mix powder with solid-phase reagent calcium hydroxide to powder, add powder matter 8 times of 80% alcohol solvent of amount soaks more than 12 hours, heating and refluxing extraction 3 hours, second of addition, 5 times of 80% ethanol of amount, adds Circumfluence distillation 2 hours, third time add 3 times of 80% ethanol of amount, heating and refluxing extraction 1 hour, filtering, merge extract solution, obtain Obtain the first extract solution.
Step 2:First extract solution is diluted with water to 4 times of amount volumes, and regulation pH value to 3.2, filtering, filtrate, which is added on, have been located In the macroporous resin column managed, first adsorbed with slower flow velocity, then rinse resin column, Ran Houyong with 5 times of amount purified waters of resin 5% sodium acetate solution of 5 times of amounts of amount of resin rinses resin column, then enters to rinse resin column with 3 times of 30% ethanol of amount of resin, most Afterwards with 80% alcohol flushing resin post of 3 times of amounts of resin, eluent is collected, after 0.2um miillpore filter refined filtration, reclaims second Alcohol, obtain the second extract solution.
Step 3:Second extract solution is concentrated, adding high concentration ethanol makes alcohol content reach 90%, with 10% hydrogen-oxygen Change sodium and adjust pH value 7.6, stand 24 hours, miillpore filter refined filtration of the supernatant through 0.2um, precipitation is also used with after centrifuge 0.2um filtering with microporous membrane, recovery ethanol add 1 times of amount purified water dilution, filled in suitable container, 110 DEG C to without alcohol taste Carry out high-temperature sterilization within 4 hours, ginkgo extract is obtained after cooling.
After measured, the ginkgo extract includes:
(a) flavone compound of 46.3% weight;
(b) ginkgolides of 8% weight;
(c) proanthocyanidin of 14% weight;
(d) ginkgoic acid of 8% weight;
(e) the polypenthylene alcohols ester of 2% weight;
(f) the alkyl phenolic acid compound of 12% weight;
Abovementioned alkyl phenolic acid compound includes protocatechuic acid, vanillic acid, caffeic acid, p- coumaric acids, forulic acid and green Ortho acid.
Contain in the ginkgo extract of this preparation embodiment 3:The polysaccharide compound of 5% weight;250ppm biflavone; The terpene lactones of 15% weight.
Also containing manganese and molybdenum trace element in the ginkgo extract obtained in this preparation embodiment 3.
Prepare embodiment 4
Step 1:Ginkgo leaf or dry grinding is taken to mix powder with solid-phase reagent sodium carbonate or sodium acid carbonate to powder, add Enter 3 times of 80% alcohol solvent of powder quality to soak more than 24 hours, heating and refluxing extraction 3 hours, second of addition, 2 times of amounts 80% Ethanol, heating and refluxing extraction 1 hour, filtering, merge extract solution, obtain the first extract solution.
Step 2:First extract solution is diluted with water to 3 times of amount volumes, and regulation pH value to 3.8, filtering, filtrate, which is added on, have been located In the macroporous resin column managed, first adsorbed with slower flow velocity, then rinse resin column, Ran Houyong with 3 times of amount purified waters of resin 5% sodium acetate solution of 5 times of amounts of amount of resin rinses resin column, then enters to rinse resin column with 3 times of 20% ethanol of amount of resin, most Afterwards with 80% alcohol flushing resin post of 3 times of amounts of resin, eluent is collected, after 0.2um miillpore filter refined filtration, reclaims second Alcohol, obtain the second extract solution.
Step 3:Second extract solution is concentrated, adding high concentration ethanol makes alcohol content reach 90%, with 10% hydrogen-oxygen Change sodium and adjust pH value 8.0, stand 24 hours, miillpore filter refined filtration of the supernatant through 0.2um, precipitation is also used with after centrifuge 0.2um filtering with microporous membrane, recovery ethanol add 0.5 times of amount purified water dilution, filled in suitable container, 120 to without alcohol taste High-temperature sterilization is carried out DEG C 3 hours, ginkgo extract is obtained after cooling.
After measured, the ginkgo extract includes:
(a) flavone compound of 45% weight;
(b) ginkgolides of 10% weight;
(c) proanthocyanidin of 9% weight;
(d) ginkgoic acid of 8% weight;
(e) the polypenthylene alcohols ester of 2% weight;
(f) the alkyl phenolic acid compound of 12% weight;
Abovementioned alkyl phenolic acid compound includes protocatechuic acid, vanillic acid, caffeic acid, p- coumaric acids, forulic acid and green Ortho acid.
Contain in the ginkgo extract of this preparation embodiment 4:The polysaccharide compound of 5% weight;220ppm biflavone; The terpene lactones of 14% weight.
Although above-described embodiment is described in detail to technical scheme, the technical side of the present invention Case is not limited to above example, in the case where not departing from the thought and objective of the present invention, to technical scheme institute Any change done falls within claims of the present invention limited range.

Claims (9)

1. a kind of ginkgo extract, it is characterised in that the extract solution includes:
(a) flavone compound of 30~50% weight;
(b) ginkgolides of 5~10% weight;
(c) proanthocyanidin of 5~15% weight;
(d) ginkgoic acid of 3~10% weight;
(e) it is less than the polypenthylene alcohols ester of 3% weight;And
(f) it is more than the alkyl phenolic acid compound of 5% weight.
2. ginkgo extract according to claim 1, it is characterised in that the flavone compound includes glycosides displayed and Huang Keto-alcohol.
3. ginkgo extract according to claim 1, it is characterised in that the ginkgolides includes ginkalide A, ginkgo Lactone B, ginkalide C, ginkgo lactones M, ginkgo lactones J, wherein content of the bilobalide J in ginkgo extract be not low In 4%.
4. ginkgo extract according to claim 1, it is characterised in that the alkyl phenolic acid compound includes former catechu Acid, vanillic acid, caffeic acid, p- coumaric acids, forulic acid and chlorogenic acid.
5. ginkgo extract according to claim 1, it is characterised in that contain in the ginkgo extract and be less than 7% weight The polysaccharide compound of amount.
6. ginkgo extract according to claim 1, it is characterised in that contain 10~25% weights in the ginkgo extract The terpene lactones of amount.
7. ginkgo extract according to claim 1, it is characterised in that micro containing manganese and molybdenum in the ginkgo extract Element.
8. ginkgo extract according to claim 1, it is characterised in that contain in the ginkgo extract and be higher than 150ppm Biflavone.
A kind of 9. method for preparing ginkgo extract described in right 1, it is characterised in that this method is made up of the following steps:
Step 1:Ginkgo leaf or dry grinding is taken to mix powder with solid-phase reagent to powder, add polar solvent immersion, heat back Raw first extract solution of miscarriage.
Step 2:First extract solution is diluted with water, and adjusts pH value 4~6, filtering, and filtrate is added in processed good macroporous resin column It is finely divided, first rinses resin column with purified water, then resin column is rinsed with sodium acetate solution, then with low concentration to high concentration Ethanol carries out gradient washes resin column, collects eluent, after miillpore filter refined filtration, obtains the second extract solution.
Step 3:Second extract solution is concentrated, and adding high concentration ethanol makes alcohol content reach 90%, is adjusted with 20% sodium hydroxide PH value, stand, supernatant also use filtering with microporous membrane through miillpore filter refined filtration after precipitation centrifuge, reclaim ethanol extremely Without alcohol taste, add purified water to dilute, fill in container, carry out high-temperature sterilization, obtain ginkgo extract.
CN201710980981.4A 2017-09-29 2017-09-29 A kind of ginkgo extract Pending CN107669529A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108913499A (en) * 2018-08-09 2018-11-30 劲牌生物医药有限公司 A kind of tartary buckwheat extract and preparation method thereof, application and health liquor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108913499A (en) * 2018-08-09 2018-11-30 劲牌生物医药有限公司 A kind of tartary buckwheat extract and preparation method thereof, application and health liquor

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Application publication date: 20180209