CN107667852A - The production method of rice paddy seed and its application - Google Patents
The production method of rice paddy seed and its application Download PDFInfo
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- CN107667852A CN107667852A CN201711054039.1A CN201711054039A CN107667852A CN 107667852 A CN107667852 A CN 107667852A CN 201711054039 A CN201711054039 A CN 201711054039A CN 107667852 A CN107667852 A CN 107667852A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The present invention relates to a kind of production method of rice paddy seed and its application.Production method comprises the following steps：S1：First rice varieties and second rice varieties are hybridized or produced character heterozygote the methods of multi items composite-crossing or biology, physics, chemistry, collect F1 generation rice heterozygosis seed；S2：F1 generation rice heterozygosis seed is sowed and cultivated, collects F2 for rice paddy seed；S3：F2 is sowed and cultivated for rice paddy seed, collects F3 for rice paddy seed；S4：F3 is sowed and cultivated for rice paddy seed, is then selfed, collects F4 for rice paddy seed；S5‑1：F4 is sowed and cultivated for rice paddy seed, colony's repeated pruning is then carried out, regeneration breeding is carried out for the rice stub in the corresponding F3 generations of colony to the F4 for reaching stability criterion；The material that regeneration breeding obtains is continued to cultivate, seed is collected after selfing, obtains the required seed of the present invention.
Description
Technical field
The present invention relates to Rice Cropping technical field, and in particular to a kind of production method of rice paddy seed and its application.
Background technology
Rice, it is annual gramineae plant, high about 1.2 meters, leaf is long and flat, and panicle is made up of many small ears.Institute
Knot reality is paddy, and rice or rice are claimed after shelling.Rice can be divided into long-grained nonglutinous rice and japonica rice, early rice and middle late rice, glutinous rice and non-glutinous
Rice.Rice, which is tied, claims rice or rice after the grain of rice shells.Rice is also used as wine brewing, sugar industry raw material, rice in addition to edible
Shell, rice straw also have many use.Rice belongs to grass family Oryza, is an extremely ancient crop.According to archaeological discovery, rice exists
The plantation history in China at least 7000 or so.Cultivated rice in the world has Asian Cultivated Rice and Oryza glaberrima Steud etc..Wherein
Asian Cultivated Rice cultivated area is big, and spread all over the world each rice region, so referred to as common cultivated rice.A host of facts prove that China is southern
Side is at least one of centre of origin of common cultivated rice.Because rice is the staple food crop of the mankind, it is reported that in the world may be used at present
140,000 kinds of rice is can exceed that, and scientist is also ceaselessly researching and developing new seed rice, to improve yield.The production of rice paddy seed is
Completed by the breeding from generation to generation of seed, the seed that this just needs each generation to produce is genetically basically identical, Cai Nengbao
The seed for holding the production of each generation shows uniformity.In general method is to allow propagating materials to be selfed eight or nine generations or more than ten
Will genetically it be stabilized after generation, it is ensured that the uniformity of each generation of propagating materials.But the method breeding algebraically is more,
Breeding cycle is grown, therefore, it is also desirable to develop the production method of new rice paddy seed.
The content of the invention
For in the prior art the defects of, present invention aims at the production method of the rice paddy seed of offer, in breeding material
Material only selfing two carries out regrowth breeding to selecting to be selfed the stable phytoma of propagating materials character after a generation after three generations again,
It can ensure that it is selfed always the being consistent property of the propagating materials after a generation again, complete the replacement of generation of kind and ensure the spy of kind
Property is constant.
To achieve the above object, technical scheme provided by the invention is：
The invention provides a kind of production method of rice paddy seed, comprise the following steps：S1：By first rice varieties and second water
Rice varieties are hybridized or produced character heterozygote the methods of multi items composite-crossing or biology, physics, chemistry, collect F1 generation
Rice heterozygosis seed；S2：F1 generation rice heterozygosis seed is sowed and cultivated, collects F2 for rice paddy seed；S3：By F2 for rice seed
Son is sowed and cultivated, and collects F3 for rice paddy seed；S4：F3 is sowed and cultivated for rice paddy seed, is then selfed, collects F4 for water
Rice；S5-1：F4 is sowed and cultivated for rice paddy seed, then carries out colony's repeated pruning, to partly reaching stable mark
Accurate F4 carries out regeneration breeding for the rice stub in the corresponding F3 generations of colony；The material that regeneration breeding obtains is continued to cultivate, after selfing
Seed is collected, finally gives the required rice paddy seed of the present invention.The inventive method also includes step：S5-2：By F4 for rice seed
Son is sowed and cultivated, and then carries out colony's repeated pruning, and the F4 to partly not reaching stability criterion collects F5 for water for colony
Rice；S6-1：F5 is sowed and cultivated for rice paddy seed, then carries out colony's repeated pruning, to partly reaching stable mark
Accurate F5 carries out regeneration breeding for the rice stub in the corresponding F4 generations of colony；The material that regeneration breeding obtains is continued to cultivate, after selfing
Collect seed, the i.e. required rice paddy seed of the present invention.
In the further embodiment of the present invention, in step S5-1 and S5-2, breed and bred for trophosome regrowth.
In the further embodiment of the present invention, in step S4 and S6-1, in F3 generations or F4, are sowed simultaneously for rice paddy seed
Cultivate obtained rice stub and carries out anniversary regeneration planting under the conditions of isolation, plant division is until regrowth reaches required incessantly
Untill seedling number.
In the further embodiment of the present invention, in step S4 and S5-2, the place of anniversary plantation can the anniversary for rice
The area of growth, including Hainan Island etc..
In the further embodiment of the present invention, in step S5 and S6-1, the seed of collection can carry out colony's production
Using.
The present invention also protects application of the production method of rice paddy seed in the restorer seed production of hybrid rice.
The present invention also protects application of the production method of rice paddy seed in the production of Dual purpose male-sterile line seed.
Technical scheme provided by the invention, there is following beneficial effect：(1) production of rice paddy seed provided by the invention
Method, propagating materials be only selfed two to after selecting after three generations it to be selfed a generation again the stable previous generation plant battalion of propagating materials character
The breeding of body regrowth is supported, so because the genotype of trophosome is consistent, it is ensured that it is selfed the propagating materials after a generation again
Always being consistent property, ensure that the characteristic of kind is constant to complete the replacement of generation of kind；(2) rice provided by the invention
The production method of seed, a breeding cycle can be completed within four to five generations, and method of the prior art needs more than ten
Generation；For the propagating materials of the method because selfing generation is few, genotype heterozygosity height should be than the breeding material of method of the prior art
Material shows stronger heterozygous advantage；(3) production method of rice paddy seed provided by the invention, the rice stub by F3 generations or F4 generations is taken
Anniversary plantation is carried out under the conditions of the area isolation in similar Hainan Island, plant division incessantly directly causes regrowth to plant several mu of causes enough
Untill seedling required for tens of mus；Key point is that original rice stub continues to leave expansion plant division to ensure to breed again in lower season with seedling, number
Mu or tens of mu of seedling institute selfed seeds make seed needed for large area production；Although this batch of seed is stablized genetically not homozygous enough,
But by the continuous plant division expanding species of the trophosome of its previous generation, the uniformity of all seeds genetically is ensure that, can keep showing from generation to generation
Type is stable, consistent；(4) production method of rice paddy seed provided by the invention, except the seed production applied to conventional rice kind
Outside, it may also be used for the production of restorer seed and the production of Dual purpose male-sterile line seed of hybrid rice；Particularly tackle Dual purpose male-sterile line
The critical temperature of sterility " drift " of seed has more special role.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Brief description of the drawings
Fig. 1 is the production method schematic diagram of the rice paddy seed in the embodiment of the present invention；
Fig. 2 is the production method schematic diagram of the rice paddy seed in comparative example of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes.The following examples are only intended to illustrate the technical solution of the present invention more clearly, therefore is intended only as example, without
It can be limited the scope of the invention with this.
Experimental method in following embodiments, it is conventional method unless otherwise specified.Examination used in following embodiments
Material is tested, is to be commercially available from regular shops unless otherwise specified.Quantitative test in following examples, is respectively provided with three
Secondary to repeat to test, data are to repeat the average value or mean+SD of experiment three times.
Embodiment
As shown in figure 1, the present embodiment provides a kind of production method of rice paddy seed, comprise the following steps：
S1：First rice varieties and second rice varieties are hybridized or multi items composite-crossing or biology, physics, chemistry etc.
Character heterozygote produced by method, collect F1 generation rice heterozygosis seed；
S2：F1 generation rice heterozygosis seed is sowed and cultivated, collects F2 for rice paddy seed；
S3：F2 is sowed and cultivated for rice paddy seed, collects F3 for rice paddy seed；
S4：F3 is sowed and cultivated for rice paddy seed, obtained rice stub will be cultivated and carry out week under the conditions of the isolation of Hainan Island
Then year plantation, incessantly plant division are selfed untill regrowth reaches required seedling number, collect F4 for rice paddy seed；
S5-1：F4 is sowed and cultivated for rice paddy seed, then carries out colony's repeated pruning, to partly reaching stable mark
Accurate F4 carries out regeneration breeding for the rice stub in the corresponding F3 generations of colony；The material that regeneration breeding obtains is continued to cultivate, after selfing
Collect seed --- as the seed alleged by the present invention.
S5-2：F4 is sowed and cultivated for rice paddy seed, then carries out colony's repeated pruning, to partly not reaching stabilization
The F4 of standard collects F5 for rice paddy seed for colony；
S6-1：F5 is sowed and cultivated for rice paddy seed, then carries out colony's repeated pruning, to partly reaching stable mark
Accurate F5 carries out regeneration breeding for the rice stub in the corresponding F4 generations of colony；The material that regeneration breeding obtains is continued to cultivate, after selfing
Collect seed --- as the seed alleged by the present invention.
Comparative example
As shown in Fig. 2 this comparative example provides a kind of production method of rice paddy seed, comprise the following steps：
S1：First rice varieties and second rice varieties are hybridized or multi items composite-crossing or biology, physics, chemistry etc.
Character heterozygote produced by method, collect F1 generation rice heterozygosis seed；
S2：F1 generation rice heterozygosis seed is sowed and cultivated, collects F2 for rice paddy seed；
S3：F2 is sowed and cultivated for rice paddy seed, collects F3 for rice paddy seed；
S4：F3 is sowed and cultivated for rice paddy seed, is then selfed, collects F4 for rice paddy seed；
S5：In repeat step S4 method more generations, F8~Fn is collected for rice paddy seed.
Planted seed is collected after step S5-1 selfings in the embodiment of the present invention, the phenotype for observing its offspring is stable
Property, result is documented in table 1 below.
Table 1 collects the phenotype for the offspring that seed is planted after being selfed
| Offspring's algebraically | Phenotypic stability |
| F4 the 1st crowd | Phenotype stable and consistent |
| F4 the 2nd crowd | Phenotype stable and consistent |
| F4 the 3rd crowd | Phenotype stable and consistent |
| F4 the 4th crowd | Phenotype stable and consistent |
| F4 the 5th crowd | Phenotype stable and consistent |
Technical scheme provided by the invention, there is following beneficial effect：(1) production of rice paddy seed provided by the invention
Method, propagating materials be only selfed two to after selecting after three generations it to be selfed a generation again the stable previous generation plant battalion of propagating materials character
The breeding of body regrowth is supported, so because the genotype of trophosome is consistent, it is ensured that it is selfed the propagating materials after a generation again
Always being consistent property, ensure that the characteristic of kind is constant with the replacement of generation without kind；(2) rice provided by the invention
The production method of seed, a breeding cycle can be completed within four to five generations, and method of the prior art needs more than ten
Generation；For the propagating materials of the method because selfing generation is few, genotype heterozygosity height should be than the breeding material of method of the prior art
Material shows stronger heterozygous advantage；(3) production method of rice paddy seed provided by the invention, the rice stub by F3 generations or F4 generations is taken
Regional isolation condition year next week in similar Hainan Island plants, and plant division incessantly directly causes regrowth to plant several mu of causes enough tens of
Untill seedling required for mu；Key point is that original rice stub continues to leave and expands plant division to ensure to breed again in lower season with seedling, several mu or
Tens of mu of seedling institute selfed seeds make seed needed for large area production；Although this batch of seed is stablized genetically not homozygous enough, lead to
The continuous plant division expanding species of trophosome of its previous generation is crossed, the uniformity of all seeds genetically is ensure that, can keep phenotype steady from generation to generation
It is fixed, consistent；(4) production method of rice paddy seed provided by the invention, in addition to being produced applied to the seed of conventional rice kind, also
The production of restorer seed and the production of Dual purpose male-sterile line seed available for hybrid rice；Particularly tackle dual-purpose and male-sterile seed
Critical temperature of sterility " drift " have more special role.
It should be noted that unless otherwise indicated, technical term or scientific terminology used in this application should be this hair
The ordinary meaning that bright one of ordinary skill in the art are understood.Unless specifically stated otherwise, otherwise illustrate in these embodiments
Part and relative step, numerical expression and the numerical value of step are not limit the scope of the invention.It is illustrated and described herein
In all examples, unless otherwise prescribed, any occurrence should be construed as merely exemplary, not as limitation, because
This, other examples of exemplary embodiment can have different values.
In the description of the invention, it is to be understood that term " first ", " second " are only used for describing " this in a large amount of kinds
One " with the meaning of " another ", and it is not intended that instruction or implying that the technical characteristic of variety name or kind must have " first "
" second ".In the description of the invention, " multiple " are meant that two or more, unless otherwise specifically defined.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, either which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme, it all should cover among protection scope of the present invention.
Claims (8)
1. a kind of production method of rice paddy seed, it is characterised in that comprise the following steps：
S1：First rice varieties and second rice varieties are hybridized or the methods of multi items composite-crossing or biology, physics, chemistry
Produced character heterozygote, collect F1 generation rice heterozygosis seed；
S2：The F1 generation rice heterozygosis seed is sowed and cultivated, collects F2 for rice paddy seed；
S3：The F2 is sowed and cultivated for rice paddy seed, collects F3 for rice paddy seed；
S4：The F3 is sowed and cultivated for rice paddy seed, is then selfed, collects F4 for rice paddy seed；
S5-1：F4 is sowed and cultivated for rice paddy seed, colony's repeated pruning is then carried out, to partly reaching stability criterion
F4 carries out regeneration breeding for the rice stub in the corresponding F3 generations of colony；The material that regeneration breeding obtains is continued to cultivate, collected after selfing
Seed, finally give the rice paddy seed.
2. the production method of rice paddy seed according to claim 1, it is characterised in that also comprise the steps：
S5-2：F4 is sowed and cultivated for rice paddy seed, colony's repeated pruning is then carried out, to partly not reaching stability criterion
F4 for colony, collect F5 for rice paddy seed；
S6-1：F5 is sowed and cultivated for rice paddy seed, colony's repeated pruning is then carried out, to partly reaching stability criterion
F5 carries out regeneration breeding for the rice stub in the corresponding F4 generations of colony；The material that regeneration breeding obtains is continued to cultivate, collected after selfing
Seed, i.e., described rice paddy seed.
3. the production method of rice paddy seed according to claim 2, it is characterised in that：
In step S5-1 and S6-1, the breeding is bred for trophosome regrowth.
4. the production method of rice paddy seed according to claim 2, it is characterised in that：
In step S4 and S5-2, the rice stub that F3 generations or F4 are sowed for rice paddy seed and cultivate to obtain is entered under the conditions of isolation
The row anniversary regeneration planting, plant division is untill regrowth reaches required seedling number incessantly.
5. the production method of rice paddy seed according to claim 2, it is characterised in that：
In step S4 and S5-2, the place of anniversary plantation is the area that rice can grow in the anniversary, including Hainan Island.
6. the production method of rice paddy seed according to claim 2, it is characterised in that：
In step S5 and S6-1, collected seed can carry out colony's production application.
7. the production method of the rice paddy seed described in claim any one of 1-6 is in the restorer seed production of hybrid rice
Using.
8. application of the production method of the rice paddy seed described in claim any one of 1-6 in the production of Dual purpose male-sterile line seed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711054039.1A CN107667852B (en) | 2017-10-31 | 2017-10-31 | Production method and application of rice seeds |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711054039.1A CN107667852B (en) | 2017-10-31 | 2017-10-31 | Production method and application of rice seeds |
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| CN107667852A true CN107667852A (en) | 2018-02-09 |
| CN107667852B CN107667852B (en) | 2020-02-18 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100671A (en) * | 2019-05-28 | 2019-08-09 | 雅安雨禾农业科技有限责任公司 | A kind of rice regeneration propagation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103782857A (en) * | 2014-01-23 | 2014-05-14 | 胡代书 | Cultivating method and seedling planting method of cross-year regrowing rice seeds |
| CN105284594A (en) * | 2015-10-20 | 2016-02-03 | 浙江绿巨人生物技术有限公司 | Selection method of bi-functional rice variety |
| CN105505986A (en) * | 2016-01-15 | 2016-04-20 | 湖南农业大学 | Method for producing ratooning rice tillering in advance and further increasing yield of ratooning rice |
-
2017
- 2017-10-31 CN CN201711054039.1A patent/CN107667852B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103782857A (en) * | 2014-01-23 | 2014-05-14 | 胡代书 | Cultivating method and seedling planting method of cross-year regrowing rice seeds |
| CN105284594A (en) * | 2015-10-20 | 2016-02-03 | 浙江绿巨人生物技术有限公司 | Selection method of bi-functional rice variety |
| CN105505986A (en) * | 2016-01-15 | 2016-04-20 | 湖南农业大学 | Method for producing ratooning rice tillering in advance and further increasing yield of ratooning rice |
Non-Patent Citations (1)
| Title |
|---|
| 李辅文: "杂交水稻再生制种技术探讨", 《种子世界》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100671A (en) * | 2019-05-28 | 2019-08-09 | 雅安雨禾农业科技有限责任公司 | A kind of rice regeneration propagation method |
| CN110100671B (en) * | 2019-05-28 | 2021-07-27 | 雅安雨禾农业科技有限责任公司 | Rice regeneration propagation method |
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| Publication number | Publication date |
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| CN107667852B (en) | 2020-02-18 |
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