CN107660541A - A kind of bactericidal composition containing indoles flusulfamide and thifluzamide - Google Patents

A kind of bactericidal composition containing indoles flusulfamide and thifluzamide Download PDF

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Publication number
CN107660541A
CN107660541A CN201610625335.1A CN201610625335A CN107660541A CN 107660541 A CN107660541 A CN 107660541A CN 201610625335 A CN201610625335 A CN 201610625335A CN 107660541 A CN107660541 A CN 107660541A
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thifluzamide
bactericidal composition
indoles flusulfamide
flusulfamide
indoles
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葛尧伦
司国栋
白复芹
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Hailir Pesticides and Chemicals Group Co Ltd
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Hailir Pesticides and Chemicals Group Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
    • A01N43/78—1,3-Thiazoles; Hydrogenated 1,3-thiazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a kind of bactericidal composition containing indoles flusulfamide and thifluzamide, its active ingredient is indoles flusulfamide and thifluzamide binary built.Wherein the mass ratio of indoles flusulfamide and thifluzamide is 1~50: 50~1, the quality parts ratio 1%~80% of active ingredient indoles flusulfamide and thifluzamide in preparation, remaining is the auxiliary element for allowing to use and receiving in agricultural chemicals, and the formulation of bactericidal composition of the present invention is missible oil, suspending agent, wettable powder, aqua, water dispersible granules, aqueous emulsion, microemulsion, granule, microcapsule formulations.It is mainly used in preventing and treating cucumber downy mildew, downy mildew of garpe, lichee phytophthora root rot and gray mold of cucumber, graw mold of tomato, wheat and the powdery mildew of barley, banded sclerotial blight etc..

Description

A kind of bactericidal composition containing indoles flusulfamide and thifluzamide
Technical field
The present invention relates to technical field of pesticide combination, more particularly to a kind of killing containing indoles flusulfamide and thifluzamide Bacteria composition.
Background technology
Indoles flusulfamide is a kind of contact killing type bactericide, there is osmotically active and ability to resist splash erosion, biochemical characteristic performance It is inhibitor inside benzoquinones in terms of the activity of inhibitory enzyme, available for preventing and treating potato phytophthora, downy mildew of garpe, cucumber downy mildew Disease, club-root etc..
Thifluzamide (thifluzamide；Chemical name：The bromo- 2- methyl -4'- trifluoromethoxies -4- fluoroforms of 2', 6'- bis- Base -1,3- thiadiazoles -5- hydroxyls anilide) belong to thiazole amide bactericide, there is thifluzamide strong uptake and translocation and length to hold Effect property.Thifluzamide causes a disease to Rhizoctonia, Puccinia, Ustilago, Tilletia, corticium, nuclear cavity Pseudomonas etc. Fungi is active, especially has special efficacy to the fungus-caused disease of Basidiomycetes such as banded sclerotial blight, damping-off etc..
It is the maximally efficient means of controlling plant diseases using chemical agent.But apply single long-term continuous high dose Chemical bactericide, easily cause the problems such as residual of medicament, environmental pollution and resistance to resistance to the action of a drug fungi are developed.Rational chemistry Bactericide compounded or mixture have expand fungicidal spectrum, improve prevention effect, extend the optimum period for applying fertilizer, reduce dosage, reduce poisoning, Reduce residual, delay the positive feature such as Antifungal resistance and drug-fast generation and development, bactericide compounded is to solve above-mentioned ask One of maximally efficient method of topic.Develop new product bactericide price is constantly soaring, and by contrast, exploitation with research efficiently, Low toxicity, the compounding of low-residual with mixture has small investment, the lead time is short and is paid attention to both at home and abroad, one after another increasing develop Dynamics.We screen and on the basis of field test, filter out indoles flusulfamide and compounded with thifluzamide indoors, have Obvious synergistic effect.And people there is no to report at present on the bactericidal composition and application of indoles flusulfamide and the compounding of thifluzamide Road mistake.
The content of the invention
Based on case above, present invention aims at provide a kind of new and effective pesticide sterilizing composite.It is mainly used in The downy mildew and phytophthora root rot for preventing and treating various crops have the effect of good, to the fungus-caused disease of Basidiomycetes such as banded sclerotial blight, Damping-off etc. has special efficacy.
Technical scheme of the present invention is realized by following measures：
A kind of bactericidal composition containing indoles flusulfamide and thifluzamide, the active ingredient indoles sulphur of the bactericidal composition Bacterium amine compounds with thifluzamide two-spot, and remaining is auxiliary element.Active ingredient indoles flusulfamide in wherein described bactericidal composition Mass ratio with thifluzamide is 1~50: 50~1, and described bactericidal composition of the present invention is through toxicity test experimental verification, indoles When the mass ratio of flusulfamide and thifluzamide is 1~10: 10~1, synergistic effect is preferable.
The formulations of pesticide that described bactericidal composition of the present invention can be prepared are missible oil, suspending agent, wettable powder, water Agent, water dispersible granules, aqueous emulsion, microemulsion, granule, microcapsule formulations.Wherein active ingredient indoles flusulfamide and thifluzamide Gross mass in the formulation accounts for the 1%~80% of the whole quality of the pharmaceutical preparations, wherein when accounting for 10%~60%, toxicity and residual reach compared with Good balance, cost are relatively low.
The specific embodiment for the formulations of pesticide that bactericidal composition of the present invention is configured to is as follows：
Described bactericidal composition is cream preparation, and the mass fraction of component is：1~50 part of indoles flusulfamide；Thiophene furan acyl 1~50 part of amine；10~30 parts of conventional emulsifier；20~50 parts of Conventional solvents；Conventional 1~5 part of synergist.The tool of the cream preparation Body production stage for first active ingredient indoles flusulfamide and thifluzamide are added in solvent be completely dissolved after add emulsifying agent, Synergist stir after into transparent and homogeneous oily liquids, it is filling, you can the cream preparation of the present composition is made.
Described bactericidal composition is suspending agent, and the mass fraction of component is：1~50 part of indoles flusulfamide；Thifluzamide 1 ~50 parts；5~20 parts of dispersant；1~5 part of antifreezing agent；0.1~2 part of thickener；0.1~0.8 part of defoamer；Penetrating agent 0~10 Part；0.1~5 part of pH value regulator；Water, surplus.The specific production stage of the suspending agent is first mixes other auxiliary agents, through at a high speed Shear-mixed is uniform, adds active ingredient indoles flusulfamide and thifluzamide, abrading-ball 2~3 hours in ball crusher, makes a diameter In below 5mm, you can the aqueous suspension formulations of the present composition are made.
Described bactericidal composition is wettable powder, and the mass fraction of component is：1~50 part of indoles flusulfamide；Thiophene furan 1~50 part of acid amides；3~10 parts of dispersant；1~5 part of wetting agent；Filler, surplus.The specific production stage of the wettable powder For：Active ingredient indoles flusulfamide is mixed with thifluzamide and dispersant, wetting agent and filler by above-mentioned formula, stirred Uniform stirring in kettle, well mixed after airslide disintegrating mill, you can the wettable powder of the present composition is made.
Described bactericidal composition is water dispersible granules, and the mass fraction of component is：1~50 part of indoles flusulfamide；Thiophene furan 1~50 part of acid amides；3~10 parts of dispersant；1~10 part of wetting agent；1~5 part of disintegrant；Filler, surplus.The water dispersible granules Specifically production stage is：By above-mentioned formula by active ingredient indoles flusulfamide and thifluzamide and dispersant, wetting agent, disintegrant And filler is well mixed, crushed with micro jet, through mediating, then add in fluidized-bed granulation dryer and made Through sampling analysis after grain, dry, screening, you can the water dispersible granules of the present composition are made.
Described bactericidal composition is aqueous emulsion, and the mass fraction of component is：1~50 part of indoles flusulfamide；Thifluzamide 1 ~50 parts；3~30 parts of emulsifying agent；5~15 parts of solvent；2~15 parts of stabilizer；1~5 part of antifreezing agent；0.1~8 part of defoamer；Increase Thick dose 0.2~2 part；Water, surplus.The specific production stage of the aqueous emulsion is：First by indoles flusulfamide and thifluzamide, solvent It is added together with emulsifying agent, cosolvent, makes to be dissolved into uniform oil phase；By others such as part water, antifreeze, antimicrobials Insecticides adjuvant mixes into uniform aqueous phase；Oil phase is added into aqueous phase while high-speed stirred in a kettle, slowly added For water until reaching phase inversion point, unlatching cutter carries out high speed shear, and adds remaining water, shearing about half an hour, forms water bag The aqueous emulsion of oil type, you can the aqueous emulsion of the present composition is made.
Described bactericidal composition is microemulsion, and the mass fraction of component is：1~50 part of indoles flusulfamide；Thifluzamide 1 ~50 parts；10~30 parts of emulsifying agent, 1~8 part of antifreezing agent, 0.5~10 part of stabilizer, 20~50 parts of Conventional solvents cosolvent.Will Indoles flusulfamide is completely dissolved with thifluzamide with cosolvent, adds the other compositions such as emulsifying agent, antifreezing agent stabilizer, uniformly Mixing, is eventually adding water, microemulsion can be made into after being sufficiently stirred.
Described bactericidal composition is granule, and the mass fraction of component is：1~50 part of indoles flusulfamide；Thifluzamide 1 ~50 parts；1~10 part of wetting dispersing agent；0.1~5 part of thickener；0.1~5 part of defoamer；Castor oil, surplus are supplied.Will be effective The each component such as composition indoles flusulfamide and thifluzamide, dispersant, stabilizer, defoamer and solvent is mixed in the ratio of formula, It is put into after being ground in sand milling kettle, is sent into mixing in mixer for well-distribution and gets product.
Described bactericidal composition is aqua, and the mass fraction of component is：1~50 part of indoles flusulfamide；Thifluzamide 1~ 50 parts；0.1~30 part of wetting dispersing agent；2~8 parts of antifreezing agent；0.1~1 part of defoamer；0.1~3 part of thickener；Spreader-sticker 5~ 15 parts；Water, surplus are supplied.Active ingredient indoles flusulfamide is completely dissolved with thifluzamide, with cosolvent, added scattered The other compositions such as agent, antifreezing agent, defoamer, uniformly mixing, is eventually adding water, after being sufficiently stirred, you can obtains finished product.
Described bactericidal composition is microcapsule formulations, and the mass fraction of component is：1~50 part of indoles flusulfamide；Thiophene furan acyl 1~50 part of amine；5~20 parts of urea；5~20 parts of formaldehyde；5~20 parts of emulsifying dispersant；1~5 part of antifreezing agent；Thickener 0.1~2 Part；0.1~0.8 part of defoamer；Water, surplus.Urea and the formaldehyde (amount of material are added in the three-necked flask equipped with agitating device Than being about l：1.5~2.0) pH value for, adjusting solution with sodium hydroxide solution then heats to 70~80 DEG C to 8~9 or so, React the urea resin prepolymer stablized.A certain amount of indoles flusulfamide is taken to be dissolved in thifluzamide in hexamethylene, and Emulsifying dispersant is added in solution, with being stirred vigorously, is made into stable as the O/W types of aqueous phase using the aqueous solution containing emulsifying dispersant Emulsion.Above-mentioned urea resin prepolymer is added in emulsion, pH value is adjusted, polymerisation occurs under acid catalysed conditions, makes Oil phase substance is wrapped, and forms microcapsule granule.Slowly heating, solidification, temperature control is at 40~50 DEG C, hardening time 1h.Selection adds appropriate auxiliary agent, you can the microcapsules that must stablize.
Wherein above-described emulsifying agent is selected from calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, alkyl phenol polyoxy Ethene ether sulfosuccinates, styrylphenol polyoxyethylene ether, NPE, castor oil polyoxyethylene ether, Any one of aliphatic acid polyethenoxy base ester, polyoxyethylene aliphatic alcohol ether or more than one any mixtures than composition.
Described solvent is dimethylbenzene or biodiesel, toluene, diesel oil, methanol, ethanol, n-butanol, isopropanol, rosin-based Vegetable oil code name is ND-45, solvent naphtha, dimethylformamide, dimethyl sulfoxide (DMSO), and the one or more in water equal solvent are appointed Anticipate than the mixture of composition.
Described dispersant is LG-3, GY-D1252, GY-D1256, SNWGF-01 selected from polycarboxylate code name, lignin Sulfonate code name is 201107,201108, APES methyl ether condensate sulfate, alkylsulfonate calcium salt, naphthalene Sulfonic formaldehyde condensation compound sodium salt, APES, polyoxyethylene carboxylate, aliphatic amine polyoxyethylene ether, glyceride One or more of fat acid ester polyoxyethylene ether.
Described wetting agent is selected from lauryl sodium sulfate, calcium dodecyl benzene sulfonate, Nekal BX, moistens bleeding agent F, Alkylbenzenesulfonate polyoxyethylene triphen phosphenylic acid salt dimly, spaonin powder, silkworm excrement, the one or more in spaonin powder.
Described disintegrant is selected from bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, in sodium acid carbonate It is one or more.
Described thickener is selected from xanthans, carboxymethyl cellulose, carboxyethyl cellulose, methylcellulose, Magnesiumaluminumsilicate, It is one or more in polyvinyl alcohol.
Described stabilizer is selected from sodium citrate, one kind in resorcinol.
Described antifreezing agent is selected from ethylene glycol, propane diols, the one or more in glycerine.
Described defoamer is selected from silicone oil, silicone compound, C10-20Saturated fat acids compound, C8-10Fatty alcohol It is one or more.
Described filler is selected from kaolin, diatomite, bentonite, attapulgite, white carbon, starch, in precipitated calcium carbonate One or more.
The present invention has obvious synergistic effect using indoles flusulfamide and composite bactericide of the thifluzamide as active ingredient, Delay the drug-fast generation in key, and reduce into production cost and use cost, be mainly used in preventing and treating the downy mildew of various crops There is the effect of good with phytophthora root rot, have special efficacy to the fungus-caused disease of Basidiomycetes such as banded sclerotial blight, damping-off etc..
Embodiment
To make technical scheme, purpose and advantage are more clearly understood, present invention specific examples below Illustrate, but the present invention is not limited to these examples.The effect experiment of the present invention uses indoor biometricses and field test phase With reference to mode, unless otherwise instructed, ratio mentioned below is all quality parts ratio.
Embodiment：Indoles flusulfamide is tested with thifluzamide different ratio co-toxicity.
1.1 reagent agent
95% thifluzamide active compound, 95% indoles flusulfamide active compound, above-mentioned active compound is by the extra large limited public affairs of rel medicine company share Research and development department is taken charge of to provide.
1.2 experiment targets
From Shandong Shouguang never applied indoles flusulfamide and the cucumber of thifluzamide and same type it is potted plant gather and separate, For trying cucumber variety：Chang Chun Mi Ci.
1.3 test method
1.3.1 medicament is prepared
Acetone solution active compound is first used, two appropriate active compounds are made into by several different ratios according to the result of preliminary experiment, It is stand-by that each processing is diluted to several concentration gradients respectively with acetone again.
By the cultivation of susceptible cucumber variety Chang Chun Mi Ci in pot for growing seedlings, it is placed in greenhouse and cultivates, plant grows to three to four leaves After phase, the blade of identical leaf age is gathered, culture and measure for cucumber downy mildew germ.
1.3.2 the culture of cucumber downy mildew germ and the preparation of spore suspension
Cucumber downy mildew germ is cultivated using plants method under conditions of 20 DEG C, 12h alternation of light and darkness, turns generation per 30d Culture 1 time.With the conidium on sterile water elution incidence of leaf during inoculation, it is l × 10 to be configured to spore concentration6Individual/mL's Suspension.
1.3.3 strains tested sensitivity testing
Toxicity test is carried out using leaf dish moisturizing method.The blade of collection is first prepared into a diameter of 1.5cm leaf dish, at random Mix, be respectively placed in the series concentration decoction configured and soak lh, each 50 leaf dishes of concentration, test with the place of not adding medicine Manage as blank control, after immersion terminates, leaf is face-up placed on the blotting paper of identical liquor strength wetting, in leaf dish Decoction blot, by the spore suspension l0L prepared be inoculated in leaf dish center, room temperature place 5min after, be placed in 20 DEG C, 1 2h Cultivated under conditions of alternation of light and darkness, the onset area in leaf dish is measured after 10d, calculates EC50。
The percentage that leaf dish area is accounted for according to lesion area divides sick level
0 grade：It is disease-free；
1 grade：Sorus area accounts for less than the 5% of whole leaf area；
3 grades：Sorus area accounts for below the 6%-10% of whole leaf area；
5 grades：Sorus area accounts for below the 11%-20% of whole leaf area；
7 grades：Sorus area accounts for below the 21%-50% of whole leaf area；
9 grades：Sorus area accounts for more than the 50% of whole leaf area.
1.3.4 mixture toxicity test and interpretation of result
The toxicity test of mixture is carried out according to mixed ratio with single dose toxicity test method.
If compare the death rate<5%, do not correct, the control death rate is corrected between 5%-20% by formula 2, is compareed The death rate>20%, experiment need to reform.
Using the logarithm value of drug concentration (mg/L) as independent variable x, using the niqueMin of corrected mortality as dependent variable y, respectively Virulence regression equation is established, the EC of single dose and each proportioning mixture is calculated using DPS softwares50Poison altogether is calculated according to the abundant methods of Sun Yun Coefficient (CTC).Co-toxicity coefficient CTC, calculation formula are as follows：(using indoles flusulfamide as standard agent, 100) its toxicity index is：
The EC of toxicity index (TI)=indoles flusulfamide of thifluzamide50The EC of/thifluzamide50×100
The EC of M true toxicity index (ATI)=indoles flusulfamide50/ M EC50×100
TI × P thiophene furans of TI × P indoles flusulfamide+thifluzamide of M theoretical toxicity index (TTI)=indoles flusulfamide Acid amides
M co-toxicity coefficient (CTC)=M ATI/M TTI × 100
In formula：
M is the mixture of indoles flusulfamide and thifluzamide different ratio
P indoles flusulfamide is indoles flusulfamide ratio shared in mixture
P thifluzamides are thifluzamide ratio shared in mixture.
2.1 toxicity test results
Toxicity test of the thifluzamide of table 1 with indoles flusulfamide to cucumber downy mildew
As can be seen from the table, the result of the test of different proportion proportioning shows, dilutes equal table respectively in active ingredient ratio Reveal stronger synergistic effect, wherein using indoles flusulfamide and thifluzamide as 1:10~10:Synergistic effect good suggestion when 1 To suitable proportion 1:10~10:1 or so mixture preparation carries out further field control effectiveness test, to evaluate its field practical application Effect.
3rd, field test preventing and treating rice sheath blight disease, the experimental result of cucumber downy mildew
3.1 field tests prevent and treat rice sheath blight disease
3.1.1 test method
Experiment chooses 4 cells, plot area 25m in the big field rice terrace in Hua Xi villages of slab bridge township2,Often processing is repeated 3 times, Random district's groups arrangement, dispenser use the hand sprayers of PB -16, plant base portion even spraying are directed at, in rice tillering latter stage plant Started medication during first meeting banded sclerotial blight scab, second of medicine is applied before rice booting latter stage cut.
3.1.2 control time and number
State of an illness radix is investigated before dispenser, strain morbidity number is investigated before second of medicine and after medicine after 10 days respectively and disease refers to.Foundation 《Pesticide field efficacy medicine test criterion》GB/17980.1-17980.53—2000.5 sampling surveys are fixed per cell diagonal, 5 clumps of rice of every investigation, every plant is investigated the state of an illness from top to bottom.
Severity Scaling standard is：
0 grade：Disease-free spot；
1 grade：3 leaves are with the ill spot of lower portion；
3 grades：3 leaves and its leaf sheath morbidity；
5 grades：2 leaves and its leaf sheath morbidity；
7 grades：Sword-like leave and its leaf sheath morbidity；
9 grades：Complete stool falls ill.
3.1.3 drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigation total number of sheets × 100
Disease index=∑ (the sick numbers of sheets at different levels × relative value of series)/(investigation total number of sheets × 9) × 100
Prevention effect (%)=(1- (disease index after disease index × treatment region medicine before blank district medicine)/(blank pair According to disease index before disease index after area's medicine × treatment region medicine)) × 100.
3.1.4 poisoning investigation method
Whether continuous 20d range estimations medicament has poisoning to crop after dispenser.
3.1.5 result of the test and analysis
The effect of each processing preventing and treating rice sheath blight disease.(table 2)
16% indoles flusulfamide thifluzamide composite bactericide controls positive effect for rice sheath blight disease as shown in Table 2 Higher than indoles flusulfamide and thifluzamide single dose, bactericidal effect is incremented by with the increase of dosage.Estimated according to field, in experiment agent In the range of amount, plant growth is normal, and each treatment agent does not occur the poisoning phenomenon to rice, and it is safety to rice to illustrate it 's.It is recommended that the bactericide different from the mechanism of action is used in mixed way to delay the drug-fast generation of germ.
3.2 field tests prevent and treat cucumber downy mildew
3.2.1 test method
Twice, equivalent clear water is sprayed in check plot for dispenser altogether for experiment.When making up a prescription, after first medicament is fully dissolved with a small amount of water, then Add suitable quantity of water and carry out complete stool spraying treatment, even spraying.Using power driven sprayer during spray, operating pressure is 0.3~ 0.4MPa, shower nozzle aperture 1.3mm.Cell spouting liquid is 40~50kg/667m2, continuous dispenser twice, 10 days intervals, by medicine Liquid uniformly sprays application to crop complete stool.
3.2.2 investigation method
Shandong Shouguang is experimental field located at, the plastic tent cucumber for selecting natural occurrence is experimental field, and soil fertility is medium, irrigation and drainage side Just.Using bilinear sampling method, take per cell at 5 points, look into 5 caves, and 2 blades in every plant of fixed middle part carry out fixed point inspection during investigation at every Look into.
It is classified according to cucumber downy mildew grade scale, record total strain number, diseased plant number, severity, calculating diseased plant rate, Disease index and preventive effect.
Cucumber downy mildew grade scale is
0 grade：Blade disease-free spot；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%~10% of whole leaf area；
5 grades：Lesion area accounts for the 11%~25% of whole leaf area；
7 grades：Lesion area accounts for the 26%~50% of whole leaf area；
9 grades：Lesion area accounts for more than the 50% of whole leaf area.
Experiment is investigated 3 times altogether, i.e., disease index is investigated before medicine, preventive effect investigation in 5 days after the 1st medicine, 5 days and 18 after 2 medicines Its preventive effect is investigated.
3.2.3 poisoning is investigated
Whether continuous 7d range estimations medicament has poisoning to crop after dispenser.
3.2.4 drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigation total number of sheets × 100
Disease index=∑ (the sick numbers of sheets at different levels × relative value of series)/(investigation total number of sheets × 9) × 100
Prevention effect (%)=(1- (disease index after disease index × treatment region medicine before blank district medicine)/(blank pair According to disease index before disease index after area's medicine × treatment region medicine)) × 100.
3.2.5 field control effectiveness test result of the test
3 each test medicine of table prevents and treats the experiment effect of cucumber downy mildew
As shown in Table 3,20% indoles flusulfamide combined with thifluzamide mixture preventing and treating cucumber downy mildew effect it is relatively aobvious Write, the significant effect of complex preparation is better than two kinds of single doses, not only significantly improves the prevention effect for downy mildew, and enter one Step extends its lasting period, and is produced under the poisoning observation of continuous 7 days without poisoning.
In summary, the bactericidal composition of the invention containing indoles flusulfamide and thifluzamide, to rice sheath blight disease, cucumber Downy mildew has good prevention effect, and it is safe to Target crops.Complex preparation not only increases preventive effect, and expands Fungicidal spectrum, use range is widened, reduce cost, multiple diseases are played a part of with a medicine is simultaneous to be controlled, mitigate manpower and materials, improve life Produce benefit.So the invention of this complex preparation is of great significance with promoting to society's tool.

Claims (4)

  1. A kind of 1. bactericidal composition containing indoles flusulfamide and thifluzamide, it is characterised in that：The bactericidal composition it is effective Composition is indoles flusulfamide and thifluzamide binary built, and remaining is auxiliary element, wherein active ingredient indoles flusulfamide and thiophene The mass ratio of ofurace is 1~50: 50~1.
  2. 2. bactericidal composition according to claim 1, it is characterised in that：Indoles flusulfamide and thifluzamide are in the formulation Gross weight accounts for the 1%~80% of the whole quality of the pharmaceutical preparations.
  3. 3. bactericidal composition according to claim 2, it is characterised in that：Indoles flusulfamide and thifluzamide are in the formulation Gross weight accounts for the 5%~60% of the whole quality of the pharmaceutical preparations.
  4. 4. according to the bactericidal composition described in claim 1 or 2 or 3, it is characterised in that：The formulation of the bactericidal composition is breast Oil, suspending agent, aqua, wettable powder, water dispersible granules, aqueous emulsion, microemulsion, granule, microcapsule formulations.
CN201610625335.1A 2016-07-30 2016-07-30 A kind of bactericidal composition containing indoles flusulfamide and thifluzamide Pending CN107660541A (en)

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