CN107649200A - Using the micro flow control chip device of PDMS material substrates - Google Patents
Using the micro flow control chip device of PDMS material substrates Download PDFInfo
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- CN107649200A CN107649200A CN201610626955.7A CN201610626955A CN107649200A CN 107649200 A CN107649200 A CN 107649200A CN 201610626955 A CN201610626955 A CN 201610626955A CN 107649200 A CN107649200 A CN 107649200A
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
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- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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Abstract
The present invention relates to a kind of micro flow control chip device using PDMS material substrates, belong to analysis testing field.The substrate of micro-fluidic chip, some problems of physical presence are made with dimethyl silicone polymer i.e. PDMS that is cheap and easily processing；Its surface of the PDMS material is strongly hydrophobic, and targetedly surface is modified or surface modification, its effect are difficult to persistently, and this case aims to solve the problem that the serial relevant issues.This case main points are, the selected PDMS with ecosystem surface of substrate, and the chain ring type magnetic holding device for being equiped with miniature ultrasonic transducer units socket is positioned at the sample liquid stream terminal of the micro-fluidic chip its neighbor positions, interfacial tension is reduced with ultrasonic wave, strong absorbability using PDMS to ultrasonic wave simultaneously, reach ultrasonic intensity rapid decrement in short distance, so as to form interfacial tension difference at the both ends of the chip, sample liquid stream is thereby facilitated to be flowed along originally hydrophobic capillary channel to the terminal direction.
Description
Technical field
The present invention relates to a kind of micro flow control chip device using PDMS material substrates, belong to analysis testing field.
Background technology
For the microflow control technique overall general picture of itself, famous micro-fluidic expert Mr. Lin Ping Cheng may refer to not
The monograph " diagram Microfluid based Lab on a chip " gone out before long, the monograph is published via Science Press, and the monograph is for miniflow
Past of control technology, now, and, vision of the future etc. etc., suffer from detail, be deep into long opinion of detail
State.
The field that micro-fluidic chip is often applied to include to containing large biological molecule sample solution carry out it is various analysis,
Detection.
The basic framework of micro-fluidic chip, including be etched with the substrate of small fluid course and fit together therewith
Cover plate, the small fluid course on the substrate, before upper cover plate is assembled, it is apparent on see to be exactly some micro-channels, to wait until
After covering cover plate thereon, just really closure forms the small fluid course, the conduit inner surface of the micro-channel together with
The part cover plate that surround the micro-channel forms described small fluid course together；So, it is clear that after assembling completes
The small fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, should
The state or property of micro-channel inner surface substantially determine the integrality or property of the small fluid course；Therefore say, this
The inner surface state or inner surface property of micro-channel of the individual structure on substrate are key factors；In principle, it is any can
The material of its solid forms is kept or kept substantially, can be used to make substrate and cover plate, such as, it can act as substrate and lid
The material of piece can be monocrystalline silicon piece, quartz plate, sheet glass, high polymer for example dimethyl silicone polymer, polymethyl methacrylate,
Makrolon etc.；Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical；From material consumption, make
From the point of view of difficulty and application popularization prospect etc. etc., not small difference, the especially choosing of that substrate between these materials be present
Material, have a great influence.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, at this
It is extremely simple that micro-channel is made on the substrate of sample, and the lower cost for material, base is made with the polydimethyl siloxane material
Piece, micro-channel, and the cover plate phase made with the cheap material such as glass or polypropylene or other plastic sheets are being suppressed or etched thereon
Coordinate, be a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap dimethyl silicone polymer
Material：So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems
It should be extremely easy to popularize, promote.
But thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong
Strong hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, that
, after overall assembling is completed, that is, after covering cover plate, because the micro-channel its inner surface in structure occupies most liquid
The inner surface of circulation road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause
Similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even in general is micro-
Pump is all difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；
Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary behaviour
Make；So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, is formed tight
Weight technology puzzlement, be another problem：PDMS polymer molecules inside its body phase of this PDMS material substrate have automatic
The characteristic diffuse to the surface, migrated, the spy that this substrate body phase inside PDMS polymer molecules are diffused to the surface, migrated automatically
Property, the state after modification by its inner surface of that micro-channel of surface modifying and decorating will be caused can not to maintain long enough
Time, the holding time for micro-channel its inner surface state after that is surface-modified be substantially only sufficient to complete laboratory internal
The time of test experiments needs；In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, it is modified
The surface state that is formed can not be lasting afterwards or after saying modification, but soon automatically tends to or say and become surface modification again
Surface state before, the strong hydrophobic surface state of that script is returned in the shorter time, then, just think, this
The micro-fluidic chip of sample can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible.This
Micro-channel on PDMS material, if not doing surface modification, similar to the aqueous solution the fine liquid stream of polar solvent can not pump it is logical
Cross, chip also cannot just use；And if having done surface modification, its state after modifying can not be persistently kept again, or together
Sample can not popularization and application.
So, how to accomplish that substrate can either be made using cheap PDMS material, and the microflute can be released
Road inner surface decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make ability
The puzzlement that the numerous professionals in domain are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic
Material surface and also have another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, until
It is heavy to be trapped within the body phase of PDMS substrates, in fact, this process, partly it is also due to PDMS material body phase interior polymer
Molecule, which has, to be diffused to the surface, caused by travel motion；Such case, it can also be explained from another angle, i.e. continue not
Disconnectedly from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be little by little by that
It has been involved in a bit by the large biological molecule of adsorption within the body phase of PDMS substrates, briefly, these adsorbed biologies
Macromolecular is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up the phenomenon of large biological molecule, its institute
Caused by influence, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up biological big point
Son, so, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, and
It is, it is constantly adsorbed, also constantly swallowed up.
On PDMS substrates, its body phase is constantly swallowed up and tests showing for associated biomolecule macromolecular in related experiment test process
As, it is to say that another kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, associated biomolecule macromolecular by after adsorption,
Depression enters these Minute pores, and then is swallowed up；However, inventor thinks, those can allow the sky of miniature scale
Qi leel squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter
Enter, both difference on yardstick are huge, must not make sweeping generalizations.Explanation is bypassed, in any case, as dependence test analysis object
Large biological molecule is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is person in charge of reception at ceremonies
Phenomenon existing for sight.
, can be from containment PDMS surfaces pair in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule
The absorption of large biological molecule addresses, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing
The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule
Swallowed up by PDMS substrate body phases；But or that old problem, that is, the chemical modification on PDMS material surface is modified
Surface state afterwards can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface, move automatically
The process of shifting, it soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge
Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS bases
Its micro-channel inner surface of piece is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can is enough
Reach and contain the absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule for a long time, and then prevent PDMS substrate body phases
The effect of swallowing up to large biological molecule so that related chip manufactured goods be able to maintain that one it is prolonged enough, reasonably guarantee the quality
Phase, it is exactly a very intractable problem.The problem equally makes this area numerous as another problem addressed above
Professional is entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.
Also be present many year in the problem, so far, also not yet properly settled.
The content of the invention
The technical problem to be solved by the invention is to provide a package solution, while solve to address above
Two problems.
The present invention solves the technical problem by following scheme, and the device that the program provides is that one kind uses PDMS materials
The micro flow control chip device of substrate, the structure of the device include micro-fluidic chip, and the structure of the micro-fluidic chip includes mutually patch
The substrate and cover plate being located at together are attached together, its test liquid of the micro-fluidic chip flows into sample end and the terminal phase of the sample fluid flow
Mutually remote, the distance between the sample introduction end and the terminal are between 3 centimetres and 10 centimetres, its sample liquid stream of the micro-fluidic chip
Sample introduction end offers sample introduction end liquid pool, and the terminal of the sample fluid flow offers terminal liquid pool, its both ends of pipeline in chip
Connected respectively with sample introduction end liquid pool and the terminal liquid pool, emphasis is that its material of the substrate is dimethyl silicone polymer material
Matter, its surface of the substrate are the surfaces of primary form, the surface of the primary form its be intended to refer to and do not pass through any surface
The surface of the primary form of the material of chemical modification or any surface chemical modification, the structure of the device also include chain ring type electricity
Magnetic holding device, six links is shared in its structure of the chain ring type magnetic holding device, the link is in strip, shaft-like or bar-shaped, each phase
Adjacent link is hinged to form chain link, the hexagonal cyclic structure of its appearance profile of the chain link, the hexagonal annular structure it is every
Its locations of structures of individual drift angle is articulated structure position, two therein links for being in opposite side position of the hexagonal annular structure
Its material is magnetic conductivity material, and its material of the remaining four processes of the hexagonal annular structure is non-magnetic material, described non-
Magnetic material refers to the material not responded strongly to externally-applied magnetic field, a ring among two links of the magnetic conductivity material
Magnet exciting coil is wound with section, the link for being wound with magnet exciting coil is respectively equiped with the both sides neighbor positions of its magnet exciting coil
The pole shoe of one magnetic conductivity material, two pole shoes being shared, two pole shoes are directed to the link of another magnetic conductivity material,
Attach to fix on its surface of the link of another magnetic conductivity material and be equiped with miniature ultrasonic transducer units, rely on electromagnetism the suction-combining force
The chain ring type magnetic holding device socket of amount is positioned at the close position of its terminal of the micro-fluidic chip, and, the higher-order of oscillation
Electric signal transmission cable, one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable link together；
The chain ring type magnetic holding device provides a function of facilitating the device to disassemble；Its major function of the miniature ultrasonic transducer units is
In the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it reduce sample solution and the micro-fluidic chip its
Interfacial tension between the inwall of inner passage, can be compatible, also, using the sample introduction end and the terminal with being somebody's turn to do
Difference in the distance between miniature ultrasonic transducer units installation position difference and its ultrasonic intensity experienced, induction
The difference formed between described its interfacial tension of sample introduction end and the terminal its interfacial tension, between the micro-fluidic chip both ends
Interfacial tension difference can form pressure gap between the both ends of the micro-fluidic chip, the pressure gap can drive sample molten
Liquid is to the end flow；The ultrasonic wave that its function of the miniature ultrasonic transducer units also includes being launched with it checks institute in sample
Its absorption on the micro-fluidic chip its inner passage inner surface of the large biological molecule contained, and then check the poly dimethyl silicon
Swallow up effect of its body phase of the substrate of oxygen alkane material to the large biological molecule；The dimethyl silicone polymer material that is soft and having elasticity
Its function of the substrate of matter includes, with its property to the strong absorption of ultrasonic wave, absorbing ultrasonic wave strongly, and thereby at this
The micro-fluidic chip terminal is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end, the end
Some high hydroscopic resin particles are filled with the liquid pool of end, its opening end of the terminal liquid pool is covered by breathable microporous film.
The word of magnet exciting coil one art-recognized meanings of itself are known.
The word of magnetic conductivity material one art-recognized meanings of itself are known；The material for belonging to magnetic conductivity material is for example pure
Iron, silicon steel, soft magnetic ferrite etc..
Non magnetic material described in this case refers to the material not responded strongly to externally-applied magnetic field, belongs to the non magnetic material
The material of matter such as metallic copper, metallic zinc, ormolu, metallic aluminium, almag etc..
The be hinged word art-recognized meanings of itself field in Machine Design is known.
Only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field,
It is known.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Its size of commercially available miniature ultrasonic transducer units
It may diminish to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface its
It is known general technology for the professional in ultrasonic technology field for body.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known skill
Art；Similarly, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also superfluous to this expansion
Speech.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator；The higher-order of oscillation electric signal transmission cable
Its other end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, come for the professional in ultrasonic technology field
Say, be simple and known；The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 2 milliwatts and 9000 milliwatts
Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
Only for the word of high hydroscopic resin one art-recognized meanings of itself, come for the professional of chemical field
Say, be known.
The high hydroscopic resin is commercially available.
Only for the word of breathable microporous film one art-recognized meanings of itself, for the professional of technical field of membrane,
It is known.
The breathable microporous film is commercially available.
This case device can further include some annexes certainly, and the annex can for example coordinate with micro-fluidic chip
The electrically or optically detection device used.The equipment that the electricity, optics etc. are used cooperatively with micro-fluidic chip, may refer to
The monograph.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as：Polypropylene,
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do
Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.
Described its thickness of cover plate and substrate can allow be any setting the thickness for being easy to assembling, the thickness of recommendation or say
Preferable thickness is between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.
It is an advantage of the invention that its close position relies on electromagnetism adhesive strength set in the terminal of the micro-fluidic chip
The described chain ring type magnetic holding device of positioning is connect, is installed with institute's attaching in the chain ring type magnetic holding device one magnetic conductivity link micro-
Type ultrasonic transducer, the low-power launched using it, the ultrasonic wave of high-frequency band so that without surface chemical modification or
It is compatible between strong hydrophobic its tube wall of micro-fluidic chip internal pipeline and the test object aqueous solution of surface chemical modification
Property be significantly increased, this is sample liquid stream by providing a realistic possibility；Meanwhile utilize dimethyl silicone polymer substrate
Its strong absorbability to ultrasonic wave, in shorter distance, it is, from the terminal to the sample introduction end
In the very short distance of only several centimeters yardsticks, reach the rapid decrement of ultrasonic intensity, thereby described in the micro-fluidic chip
The difference of the interfacial tension is caused at both ends, and then, using the interfacial tension between the both ends difference its formed this two
Pressure gap between end, driving sample liquid stream is in the strong hydrophobic capillary channel of such a script to the terminal side
To flowing.By this case liquid stream drive scheme, entirely without must be to the substrate and its internal pipeline of the dimethyl silicone polymer material
Any surface chemical modification or chemical modification are carried out, has altogether dispensed with the trouble journey of the surface chemical modification or chemical modification
Sequence；And the equipment of traditional Micropump etc is altogether dispensed with；On the other hand, the low-power, the ultrasound of high-frequency band
Ripple, additionally it is possible to contain large biological molecule in sample in literalness naked its inner surface of pipeline of dimethyl silicone polymer substrate
On absorption, and then contain the swallow up effect of its body phase of dimethyl silicone polymer substrate to the large biological molecule；By institute
The suction-operated and the described effect of swallowing up stated effectively are contained that therefore, dependence test result will be better able to objectively
Reflect actual conditions.
Due to the surface chemical modification for the dimethyl silicone polymer substrate its relevant surfaces or surface need not be carried out
Chemical modification operates, and therefore, this surface chemical modification layer or surface chemical modification layer not need to exist, then, should
Its body phase interior polymer molecule of dimethyl silicone polymer substrate constantly diffuses to the surface automatically, migrate caused by it to described
The damaging influence of surface chemical modification layer or surface chemical modification layer is also just not present.
It is related that the technical scheme of this case has dissolved its application to dimethyl silicone polymer substrate addressed above totally
A series of technical barriers.Based on this case scheme, the very cheap polydimethyl siloxane material of this kind is just possible to micro- at this
It is prepared by fluidic chip, production, using etc. field play bigger effect.
Attached in the chain ring type magnetic holding device one magnetic conductivity link in this case structure it is fixed installed it is described miniature
Ultrasonic transducer, this architecture provides a function of facilitating the device to disassemble, in this way, the chain ring type magnetic holding device is together with it
The miniature ultrasonic transducer units attached in one magnetic conductivity link just easily can be mutually disengaged with the micro-fluidic chip, that
, the component that the part can freely depart from reuses many times in which just can circulate benignly；The architectural feature is advantageous to save
The use cost of the device.
Brief description of the drawings
Fig. 1 be this case device chain ring type magnetic holding device its with appearance side rough under the micro-fluidic chip socket state
View, the legend and indicates the relative position relation under the socket state between each component.
Fig. 2 is the outside side view under another viewing angle of this case device；Fig. 2 viewing angle and Fig. 1's
Viewing angle is mutually perpendicular to.
In figure, 1 is chain ring type magnetic holding device, and 2 be the terminal of the micro-fluidic chip, and 3 be the institute of the micro-fluidic chip
Sample introduction end is stated, 4 be the substrate of dimethyl silicone polymer material, and 5 be cover plate, and 6 be miniature ultrasonic transducer units, and 7 be the higher-order of oscillation
Electric signal transmission cable, 8,15 respectively be positioned at different structure position two magnetic conductivity materials link, two magnetic conductivity
The link of material end-to-end relation each other in the structure shown here, 9,22 be two pole shoes positioned at different structure position respectively, 10,12,
14th, 17,19,21 be six pin joints in six diverse locations respectively, and 11,13,18,20 be to be in four different positions respectively
The link for the four non magnetic materials put, 16 be magnet exciting coil；The chain ring type magnetic holding device structure in legend is only signal
Legend structure, the actual chain ring type magnetic holding device structure are not limited to the legend chain ring type magnetic holding device structure；Arrow in legend
The symbology micro-fluidic chip its in actual motion, driven by pressure at two ends difference, the flow direction of its sample liquid stream.
Embodiment
In this case that Fig. 1 is shown embodiment, the structure of the device includes micro-fluidic chip, the micro-fluidic chip
Structure includes being bonded to each other the substrate 4 and cover plate 5 of installing together, and its test liquid of the micro-fluidic chip flows into sample end 3 and the examination
The terminal 2 of sample fluid flow is located remotely from each other, the distance between the sample introduction end 3 and the terminal 2 between 3 centimetres and 10 centimetres,
Its test liquid of the micro-fluidic chip flows into sample end and offers sample introduction end liquid pool, and the terminal of the sample fluid flow offers terminal liquid
Pond, its both ends of pipeline in chip connect with sample introduction end liquid pool and the terminal liquid pool respectively, and emphasis is, the substrate 4 its
Material is dimethyl silicone polymer material, and its surface of substrate 4 is the surface of primary form, its meaning of the surface of the primary form
Refer to not by any surface chemical modification or the surface of the primary form of the material of any surface chemical modification, the dress
The structure put also includes chain ring type magnetic holding device 1, shared in its structure of the chain ring type magnetic holding device 1 six links 8,11,13,
15th, 18,20, in strip, shaft-like or bar-shaped, each adjacent link is hinged to form chain the link 8,11,13,15,18,20
Ring, the hexagonal cyclic structure of its appearance profile of the chain link, each drift angle 10 of the hexagonal annular structure, 12,14,17,19,
21 its locations of structures are articulated structure position, two therein links 8,15 for being in opposite side position of the hexagonal annular structure
Its material is magnetic conductivity material, and its material of the remaining four processes 11,13,18,20 of the hexagonal annular structure is non magnetic
Material, the non magnetic material refer to the material not responded strongly to externally-applied magnetic field, two links of the magnetic conductivity material
Among a link 15 on be wound with magnet exciting coil 16, this is wound with the link 15 of magnet exciting coil 16 in its magnet exciting coil 16
The pole shoe of a magnetic conductivity material is respectively equiped with the neighbor positions of both sides, shares two pole shoes 9,22, two pole shoes 9,
22 are directed to the link 8 of another magnetic conductivity material, and fixed installing is attached on its surface of the link 8 of another magnetic conductivity material
There are miniature ultrasonic transducer units 6, rely on the chain ring type magnetic holding device 1 socket of electromagnetism adhesive strength to be positioned at the micro-fluidic chip
The close position of its terminal 2, and, higher-order of oscillation electric signal transmission cable 7, the higher-order of oscillation electric signal transmission cable 7
One end linked together with the miniature ultrasonic transducer units 6；The chain ring type magnetic holding device 1 provides one and facilitates the device to tear open
The function of solution；Its major function of the miniature ultrasonic transducer units 6 is in the actual sample introduction test of micro-fluidic chip, is sent out using it
The ultrasonic wave penetrated reduces the interfacial tension between sample solution and the inwall of its inner passage of micro-fluidic chip, can
It is compatible, also, utilize the sample introduction end 3 and the distance between the terminal 2 and the installation position of miniature ultrasonic transducer units 6
Difference in difference and its ultrasonic intensity experienced, its interfacial tension of sample introduction end 3 described in induced synthesis and the end
The difference between 2 its interfacial tension is held, the interfacial tension difference between the micro-fluidic chip both ends 2,3 can be in the micro-fluidic core
Pressure gap is formed between the both ends 2,3 of piece, the pressure gap can drive sample solution to be flowed to the direction of terminal 2；Should
The ultrasonic wave that miniature ultrasonic transducer units 6 its functions also includes being launched with it check large biological molecule contained in sample its
Absorption on the micro-fluidic chip its inner passage inner surface, so check the dimethyl silicone polymer material substrate 4 its
Swallow up effect of the body phase to the large biological molecule；It is soft and have the substrate 4 of the dimethyl silicone polymer material of elasticity its function
Including with its property to the strong absorption of ultrasonic wave, being absorbed strongly to ultrasonic wave, and thereby at the micro-fluidic chip end
End 2 is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end 3, filling in the terminal liquid pool
There are some high hydroscopic resin particles, its opening end of the terminal liquid pool is covered by breathable microporous film.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its try
The flow direction of sample liquid stream.
The associate members such as the higher-order of oscillation electric signal generator are depicted without in Fig. 1 and Fig. 2.
Involved magnetic conductive material and involved nonmagnetic substance market are on sale.
Involved miniature ultrasonic transducer units 6 are commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 7 is commercially available；Can also be special to ultrasonic transducer producer or cable
Industry producer customizes.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also be customized to relevant manufacturers
Its inner passage of the micro-fluidic chip involved by this case is the pipeline of hydrophobic capillary form.
Claims (5)
1. using the micro flow control chip device of PDMS material substrates, the structure of the device includes micro-fluidic chip, the micro-fluidic core
The structure of piece includes being bonded to each other the substrate and cover plate of installing together, and its test liquid of the micro-fluidic chip flows into sample end and the examination
The terminal of sample fluid flow is located remotely from each other, and between 3 centimetres and 10 centimetres, this is micro- for the distance between the sample introduction end and the terminal
Its test liquid of fluidic chip flows into sample end and offers sample introduction end liquid pool, and the terminal of the sample fluid flow offers terminal liquid pool,
Its both ends of pipeline in chip connect with sample introduction end liquid pool and the terminal liquid pool respectively, it is characterised in that the substrate its
Material is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, its meaning of the surface of the primary form
Refer to not by any surface chemical modification or the surface of the primary form of the material of any surface chemical modification, the dress
The structure put also includes chain ring type magnetic holding device, shares six links in its structure of the chain ring type magnetic holding device, the link is equal
In strip, shaft-like or bar-shaped, each adjacent link is hinged to form chain link, the hexagonal ring-type knot of its appearance profile of the chain link
Structure, its locations of structures of each drift angle of the hexagonal annular structure is articulated structure position, and the hexagonal annular structure is wherein
Two in the link of opposite side position its material be magnetic conductivity material, its material of the remaining four processes of the hexagonal annular structure
Matter is non-magnetic material, and the non magnetic material refers to the material not responded strongly to externally-applied magnetic field, the magnetic conductivity material
Magnet exciting coil is wound with a link among two links of matter, this is wound with the link of magnet exciting coil in its magnet exciting coil
Both sides neighbor positions on be respectively equiped with the pole shoe of a magnetic conductivity material, share two pole shoes, two pole shoes refer both to
Attach to fix to the link of another magnetic conductivity material, on its surface of the link of another magnetic conductivity material and be equiped with miniature surpass
Acoustic wave transducer, the chain ring type magnetic holding device socket of electromagnetism adhesive strength is relied on to be positioned at the micro-fluidic chip its described terminal
Close position, and, higher-order of oscillation electric signal transmission cable, one end of the higher-order of oscillation electric signal transmission cable is miniature with this
Ultrasonic transducer links together；The chain ring type magnetic holding device provides a function of facilitating the device to disassemble；This is miniature
Its major function of ultrasonic transducer is in the test of micro-fluidic chip actual sample introduction, and the ultrasonic wave launched using it is reduced
Interfacial tension between sample solution and the inwall of its inner passage of micro-fluidic chip, can be compatible, also, utilizes institute
State the distance between sample introduction end and the terminal and miniature ultrasonic transducer units installation position difference and it is experienced
Ultrasonic intensity on difference, the difference between its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension
Different, the interfacial tension difference between the micro-fluidic chip both ends can form pressure differential between the both ends of the micro-fluidic chip
Different, the pressure gap can drive sample solution to the end flow；Its function of the miniature ultrasonic transducer units also includes with it
The ultrasonic wave launched check in sample contained large biological molecule its on the micro-fluidic chip its inner passage inner surface
Absorption, and then check swallow up effect of its body phase of the substrate of the dimethyl silicone polymer material to the large biological molecule；It is soft
And the substrate of the dimethyl silicone polymer material its function of having elasticity is included with its property to the strong absorption of ultrasonic wave, to super
Sound wave is absorbed strongly, and thereby real within micro-fluidic chip terminal to the limited short distance between the sample introduction end
The rapid decrement of existing ultrasonic intensity, the terminal liquid pool is interior to be filled with some high hydroscopic resin particles, its opening of the terminal liquid pool
End is covered by breathable microporous film.
2. the micro flow control chip device according to claim 1 using PDMS material substrates, it is characterised in that the cover plate
Its material is dimethyl silicone polymer material.
3. the micro flow control chip device according to claim 1 using PDMS material substrates, it is characterised in that the cover plate
And substrate its thickness is between 1.0 millimeters and 5.0 millimeters.
4. the micro flow control chip device according to claim 1 using PDMS material substrates, it is characterised in that the device
Structure also includes higher-order of oscillation electric signal generator, its other end of the higher-order of oscillation electric signal transmission cable and the higher-order of oscillation
Electric signal generator connects.
5. the micro flow control chip device according to claim 1 using PDMS material substrates, it is characterised in that this is miniature super
Its specified ultrasonic wave transmission power of acoustic wave transducer between 2 milliwatts and 9000 milliwatts, the miniature ultrasonic transducer units its
The frequency for the ultrasonic wave launched during operation is between 100KHz and 12MHz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610626955.7A CN107649200A (en) | 2016-07-26 | 2016-07-26 | Using the micro flow control chip device of PDMS material substrates |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610626955.7A CN107649200A (en) | 2016-07-26 | 2016-07-26 | Using the micro flow control chip device of PDMS material substrates |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817495A (en) * | 2010-03-25 | 2010-09-01 | 湖南大学 | Micro fluid control chip and preparation method and application thereof |
| CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817495A (en) * | 2010-03-25 | 2010-09-01 | 湖南大学 | Micro fluid control chip and preparation method and application thereof |
| CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
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| JAMES FRIEND ETAL.: "Microscale acoustofluidics:microfluidics driven via acoustics and ultrasonics", 《REVIEWS OF MODERN PHYSICS》 * |
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