CN107646807B

CN107646807B - Drosophila capturing device

Info

Publication number: CN107646807B
Application number: CN201711018042.8A
Authority: CN
Inventors: 沈锡权; 陈俊粤
Original assignee: Ningbo University
Current assignee: Ningbo University
Priority date: 2017-10-23
Filing date: 2017-10-23
Publication date: 2023-09-29
Anticipated expiration: 2037-10-23
Also published as: CN107646807A

Abstract

The invention discloses a fruit fly capturing device, which consists of a fruit fly catcher and a fruit fly observing chamber which are detachably connected; the fly catcher comprises a fly hood, a blocking sliding sheet and a fly-catching rod used for driving flies blocked in a cavity formed by the fly hood and the blocking sliding sheet into a fly observation chamber communicated with the fly hood, wherein the fly hood is used for forming a fly inlet on the side wall attached to a fly culture container, the blocking sliding sheet is in sliding connection with the inner wall of the fly inlet side of the fly hood, and the fly-catching rod is in sliding connection with the cavity wall of the fly hood; the advantage lies in that after the drosophila is covered by the drosophila cover attached drosophila culture container, the drosophila is blocked by pushing the blocking sliding sheet, and then under the pushing of the fly-catching rod, the drosophila is driven into the drosophila observation container with narrow space, thereby limiting the drosophila in a smaller movable range, and further obtaining better observation effect.

Description

Drosophila capturing device

Technical Field

The invention relates to an experimental biological capturing device, in particular to a fruit fly capturing device.

Background

Drosophila is a model organism commonly used in biochemical laboratories, and is typically cultivated and propagated in a culture vessel. The drosophila needs to be transferred from the culture vessel to the experimental vessel before the experiment is performed. When the fruit fly is transferred from the culture container, the fruit fly is generally transferred by ether vignetting, but the method has the defects that the consumption of ether is difficult to grasp, the consumption is small, the fruit fly cannot be vignetting, the fruit fly is easy to die when the consumption is large, even if the fruit fly is recovered after the fruit fly is successfully transferred, the fruit fly is recovered, the fruit fly is damaged after the ether vignetting, and some fruit flies die within 1-3 days after recovery; namely, the use of the vignetting to transfer the drosophila is unfavorable for the health of the drosophila, which hinders the experimental process.

Drosophila has the nature of climbing upwards, and related researchers take advantage of this property to transfer through container docking, which does not harm the Drosophila. Some active drosophila will climb from the culture container to the experimental container after the container is docked, but some drosophila will prefer to lie on the wall of the culture container and cannot finish the transfer, so a tool capable of capturing drosophila from the wall of the culture container is urgently needed.

Disclosure of Invention

The technical problem to be solved by the invention is to provide a drosophila capturing device for capturing drosophila lying on the wall of a culture container.

The technical scheme adopted for solving the technical problems is as follows: a fruit fly catching device consists of a fruit fly catcher and a fruit fly observing chamber which are detachably connected; the utility model provides a fly catcher, including fruit fly hood and shutoff gleitbretter to and be used for to be blocked in the fruit fly of the cavity that fruit fly hood and shutoff gleitbretter enclose drive into the fly rod of the fruit fly observation chamber with fruit fly hood intercommunication, the fruit fly entry has been seted up to the lateral wall that fruit fly hood was used for laminating with fruit fly culture vessel, shutoff gleitbretter and fruit fly hood fruit fly entry side inner wall sliding connection, fly rod and fruit fly hood chamber wall sliding connection.

Preferably, the plurality of fruit fly observation containers are arranged, the bottom of the fruit fly catcher is provided with a compartment corresponding to the fruit fly observation container, and the bottom of the compartment is provided with a channel connected with the fruit fly observation container, so that the separation of fruit fly individuals is facilitated; the fly-expelling rod comprises a main push rod and an auxiliary fly-expelling rod corresponding to the compartment, wherein the main push head of the main push rod is in sliding connection with the cavity wall of the fruit fly hood, and the sub-push head of the auxiliary fly-expelling rod is integrally arranged with the push rod penetrating through the main push rod main push head. The method can separate and drive the drosophila into a smaller drosophila observation container independently, and obtain better observation effect after fixing the drosophila.

Preferably, the bottom of the push head is provided with bristles which can penetrate through corresponding channels and extend into the fruit fly observation container. The brush hair is soft, and the damage to the fruit fly is avoided, which is beneficial to the subsequent experiment.

Preferably, the fruit fly observation container comprises a transparent fruit fly observation chamber and a sleeve communicated with the fruit fly observation chamber, wherein the inner wall of the sleeve is in interference fit connection with the outer wall of the channel, and the sleeve is provided with a switch valve. Preventing the drosophila from escaping from the detached drosophila observation container.

Preferably, a movable positioning wall is arranged on the fruit fly observation chamber, protruding blocks are arranged at two sides of the movable positioning wall, and guide grooves corresponding to the protruding blocks are arranged on the observation walls at two sides of the movable positioning wall. The moving space of the drosophila can be gradually reduced, the drosophila can be finally fixed for convenient observation, and the living state can be maintained after the observation, so that healthy target drosophila can be selected and recovered in the next step.

Preferably, the fruit fly observation chamber is provided with a movable observation wall, and the worm wheel connected to the movable observation wall through a bearing is matched with the worm fixed at the bottom of the fruit fly observation chamber. The drosophila is forced to be attached to the observation wall, so that the properties of the drosophila can be observed better. The moving space of the drosophila melanogaster can be gradually reduced, the drosophila melanogaster can be finally fixed and kept in a living state so as to be convenient for observation, and the living state can be kept after the observation, thereby being beneficial to the next selection and recovery of healthy target drosophila melanogaster.

Compared with the prior art, the invention has the advantages that after the drosophila hood is attached to the drosophila cultivation container to cover the drosophila, the drosophila is blocked by pushing the blocking sliding sheets, then the drosophila is driven into the channel of the drosophila hood by pushing the drosophila driving rod, the drosophila is separated among the drosophila individuals, the drosophila is finally driven into the drosophila observation container with a narrow space by pushing the auxiliary drosophila driving rod, the drosophila is limited in a smaller moving range, then the drosophila catcher is separated from the drosophila observation chamber, and the drosophila observation chamber fixed with the drosophila is placed under the dissecting mirror or the microscope for observation, so that a better observation effect can be obtained, and after observation, the drosophila is driven to move the drosophila again, and healthy target drosophila is selected and recovered.

Drawings

Fig. 1 is a schematic cross-sectional view of the present invention.

Fig. 2 is a schematic bottom view of the drosophila shield of the present invention.

FIG. 3 is an enlarged schematic view of the observation chamber for fruit fly according to the present invention.

Detailed Description

The invention is described in further detail below with reference to the embodiments of the drawings.

The preferred embodiment is shown in fig. 1 to 3, which are a fruit fly capturing device, comprising a fruit fly catcher 1 and a fruit fly observing container 2 which are detachably connected; the drosophila catcher 1 comprises a drosophila hood 11, a drosophila-driving rod 12 and a blocking slide sheet 13, wherein the drosophila-viewing container 2 is communicated with the drosophila hood 11, and the drosophila-driving rod 12 is used for driving drosophila blocked in a cavity enclosed by the drosophila hood 11 and the blocking slide sheet 13 into the drosophila-viewing container 2. The drosophila shield 11 is used for being provided with the drosophila inlet 111 with the lateral wall that the drosophila cultivates the container laminating, wherein, shutoff gleitbretter 13 and drosophila shield 11 drosophila inlet 111 side inner wall sliding connection, catch up with fly rod 12 and drosophila shield 11 chamber wall sliding connection. After the drosophila shield 11 is attached to the drosophila culture container, the blocking sliding sheet 13 is pushed, the shielded drosophila is driven into a cavity surrounded by the drosophila shield 11 and the blocking sliding sheet 13 from the drosophila inlet 111, then the drosophila driving rod 12 is pushed to drive the drosophila into the drosophila observation container 2, and the drosophila observation container 2 is detached under a microscope to carry out biochemical observation of the drosophila.

In this embodiment, there are nine fruit fly observation containers 2, and the specific number of fruit fly observation containers 2 may be set according to the size of the fruit fly hood 11; at the bottom of the fruit fly catcher 1, there is a compartment corresponding to the fruit fly observation container 2, and at the bottom of the compartment, there is a channel 112 connected to the fruit fly observation container 2, and the compartment separates the individual fruit flies.

Wherein, the fly-driving rod 12 comprises a main push rod 121 and an auxiliary fly-driving rod 122 corresponding to the compartment, the main push head 1211 of the main push rod 121 is slidably connected with the cavity wall of the drosophila hood 11, and the sub-push head 1221 of the auxiliary fly-driving rod 122 is integrally arranged with the push rod passing through the main push head 1211 of the main push rod 121. The bottom of the push head 1221 is provided with bristles 1222 that can extend through the corresponding channel 112 into the drosophila viewing container 2.

Further, the fruit fly observation container 2 comprises a transparent fruit fly observation chamber 21 and a sleeve 22 communicated with the fruit fly observation chamber 21, wherein the inner wall of the sleeve 22 is in interference fit connection with the outer wall of the channel 112, the sleeve 22 is provided with a switch valve 23, and the switch valve 23 is closed after the fruit fly is driven into the fruit fly observation chamber 21. The fruit fly observation chamber 21 is provided with a movable positioning wall 211, two sides of the movable positioning wall 211 are provided with convex blocks, and the observation walls at two sides of the movable positioning wall 211 are provided with guide grooves corresponding to the convex blocks. The fruit fly observation chamber 21 is provided with a movable observation wall 212, and a worm wheel 214 connected to the movable observation wall 212 through a bearing 215 is matched with a worm 213 fixed at the bottom of the fruit fly observation chamber 21.

Claims

1. A fruit fly catching device, which is characterized in that: consists of a drosophila catcher (1) and a drosophila observing container (2) which are detachably connected; the fly catcher (1) comprises a fly hood (11) and a blocking slide sheet (13), and a fly expelling rod (12) used for expelling flies blocked in a cavity surrounded by the fly hood (11) and the blocking slide sheet (13) into a fly observing container (2) communicated with the fly hood (11), wherein a fly inlet (111) is formed in the side wall of the fly hood (11), which is used for being attached to a fly culturing container, of the fly catcher, the blocking slide sheet (13) is in sliding connection with the side inner wall of the fly inlet (111) of the fly hood (11), and the fly expelling rod (12) is in sliding connection with the cavity wall of the fly hood (11);

the fruit fly observation container (2) is provided with a plurality of compartments corresponding to the fruit fly observation container (2) at the bottom of the fruit fly catcher (1), and a channel (112) connected with the fruit fly observation container (2) is arranged at the bottom of each compartment; the fly-expelling rod (12) comprises a main push rod (121) and auxiliary fly-expelling rods (122) corresponding to the compartments, a main push head (1211) of the main push rod (121) is in sliding connection with the cavity wall of the drosophila hood (11), and a sub-push head (1221) of the auxiliary fly-expelling rods (122) is integrally arranged with a push rod penetrating through the main push head (1211) of the main push rod (121);

the bottom of the push head (1221) is provided with bristles (1222) which can penetrate through the corresponding channels (112) and extend into the fruit fly observation container (2);

the fruit fly observation container (2) comprises a transparent fruit fly observation chamber (21) and a sleeve (22) communicated with the fruit fly observation chamber (21), wherein the inner wall of the sleeve (22) is in interference fit connection with the outer wall of the channel (112), and the sleeve (22) is provided with a switch valve (23);

the fruit fly observation chamber (21) is provided with a movable positioning wall (211), two sides of the movable positioning wall (211) are provided with convex blocks, and the observation walls at two sides of the movable positioning wall (211) are provided with guide grooves corresponding to the convex blocks;

the fruit fly observation chamber (21) is provided with a movable observation wall (212), and a worm wheel (214) connected to the movable observation wall (212) through a bearing (215) is matched with a worm (213) fixed at the bottom of the fruit fly observation chamber (21).