CN107646686A - A kind of expanding propagation method of sugarcane detoxication tissue culturing seedling - Google Patents
A kind of expanding propagation method of sugarcane detoxication tissue culturing seedling Download PDFInfo
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- CN107646686A CN107646686A CN201711055843.1A CN201711055843A CN107646686A CN 107646686 A CN107646686 A CN 107646686A CN 201711055843 A CN201711055843 A CN 201711055843A CN 107646686 A CN107646686 A CN 107646686A
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- tissue culturing
- sugarcane
- culturing seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses the object of the present invention is achieved like this, a kind of expanding propagation method of sugarcane detoxication tissue culturing seedling, comprise the following steps：A selects suitable culture medium；B determines the numerous multiple of the expansion of sugarcane detoxication tissue culturing seedling；C determines the numerous culture intensity of illumination of the expansion of sugarcane detoxication tissue culturing seedling；D determines temperature needed for the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling；The time required to E determines the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling.The present invention improves the quality of sugarcane detoxication tissue culturing seedling, becomes secondary shifting once to transplant to produce high-quality rooted seedling, is that sugarcane detoxication tissue culturing seedling is disposably directly applied to create necessary condition into the production of squeezing sugarcane.
Description
Technical field
The invention belongs to planting technology field, and in particular to a kind of expanding propagation method of sugarcane detoxication tissue culturing seedling.
Background technology
Sugarcane, (scientific name：Sanccharum officenarum L) saccharum, perennial tall and big solid draft.Sugarcane stem
It is sturdy tall and big.High 3-5 (- 6) rice of stalk.The southern torrid areas such as TaiWan, China, Fujian, Guangdong, Hainan, Guangxi, Sichuan, Yunnan is wide
General plantation.Sugarcane is adapted to plant in fertile soil, place sunny, summer in the winter temperature difference is big.Sugarcane is temperate zone and tropical farming
Thing, it is the raw material for manufacturing sucrose, and ethanol can be refined as energy substitution product.The whole world has individual country more than 100 to produce sugarcane,
Maximum sugarcane production state is Brazil, India and China.Containing abundant sugar, moisture in sugarcane, also contain newly old to human body
The materials such as the various vitamins being highly profitable, fat, protein, organic acid, calcium, iron are metabolized, are mainly used in refining sugar, epidermis is general
For purple and green two kinds of common colors, also there is red and brown, but it is more rare.
Sugarcane, due to planting repeatedly for many years, is highly prone to seedling biography as the crop that vegetative propagation is carried out with sugarcane stem axillary bud
The dip-dye repeatedly (such as ratoon stunting disease, mosaic disease and smut) of pathogeny is broadcast, causes yield and quality to decline, so as to cause place
The shortening of the root time limit and the degeneration of kind property.The seedling disease that (poison) is propagated in spite of illness is prevented, most economical effective measures are exactly to give birth to
Production, breeding and popularization virus-elimination seedlingses.Under this theoretical direction, partly solves the problems, such as seedling detoxification (tissue culture detoxification before
Seedling and stem section detoxic seedling), but after this tissue culture detoxic seedling seeds into the ground, to pass through triennium can just recover character, arrive
Just the tissue-cultured seedling cane of recovery character can be used for the production into squeezing sugarcane within 4th year.Can not solve disposably to be directly used in into squeezing
The problem of sugarcane produces.
Nursery lot that the also limited amount of stem section detoxic seedling, only footpath spend 3 years expand it is numerous after, can just throw within the 4th year
Enter to enter squeeze sugarcane production in, can not solve the problems, such as disposably to be directly used in and produced into squeezing sugarcane, both detoxic seedlings pass through
After the plantation of 3 years nursery lots, or there is two large problems, first, detoxic seedling has restarted susceptible, simply viral ratio does not have
Have took off poison it is light a bit, more than 1 year will be shortened at least to the perennial root time limit；Second, it can not still break away from the biography for making kind with sugarcane stem
System cultivation mode, every kind of one mu of sugarcane are planted with one ton or so sugarcane stem, cause the waste and the work of carrying and sowing of sugarcane stem
Intensity is very big, so a kind of production technology for the sugarcane detoxication tissue culturing seedling that can solve the problem that above-mentioned two problems of exploitation and this kind of group
The field planting technology of training seedling is very important.Wherein in the production technology of sugarcane detoxication tissue culturing seedling, " sugarcane detoxication tissue culturing seedling
Expanding propagation method " be important composition therein part.
The content of the invention
It is an object of the invention to provide a kind of expanding propagation method of sugarcane detoxication tissue culturing seedling.
The object of the present invention is achieved like this, a kind of expanding propagation method of sugarcane detoxication tissue culturing seedling, comprises the following steps：
A selects suitable culture medium；
B determines the numerous multiple of the expansion of sugarcane detoxication tissue culturing seedling；
C determines the numerous culture intensity of illumination of the expansion of sugarcane detoxication tissue culturing seedling；
D determines temperature needed for the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling；
The time required to E determines the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling.
Preferably, the numerous multiple of expansion described in step B is 1-5 times.
Preferably, the intensity of illumination described in step C is not less than 2000LX.
Preferably, temperature needed for the numerous culture of expansion described in D steps is 25 DEG C -35 DEG C.
Preferably, it is 15-30 days the time required to the numerous culture of expansion described in E steps.
The present invention improves the quality of sugarcane detoxication tissue culturing seedling, becomes secondary shifting to produce high-quality rooted seedling once to transplant,
For sugarcane detoxication tissue culturing seedling is disposably directly applied to necessary condition is created into the production of squeezing sugarcane.
Embodiment：
Make explanation preferably to the present invention with reference to embodiment, but the present invention be not any limitation as in any way,
Based on present invention teach that any conversion or replacement made, belong to protection scope of the present invention.
Embodiment 1
A kind of expanding propagation method of sugarcane detoxication tissue culturing seedling, comprises the following steps：
A selects suitable culture medium；
B determines the numerous multiple of the expansion of sugarcane detoxication tissue culturing seedling；
C determines the numerous culture intensity of illumination of the expansion of sugarcane detoxication tissue culturing seedling；
D determines temperature needed for the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling；
The time required to E determines the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling.
Preferably, the numerous multiple of expansion described in step B is 1 times.
Preferably, the intensity of illumination described in step C is not less than 2000LX.
Preferably, temperature needed for the numerous culture of expansion described in D steps is 25 DEG C.
Preferably, it is 15 days the time required to the numerous culture of expansion described in E steps, what is obtained is that subculture expands numerous tissue-cultured seedling.
Embodiment 2
A kind of expanding propagation method of sugarcane detoxication tissue culturing seedling, comprises the following steps：
A selects suitable culture medium；
B determines the numerous multiple of the expansion of sugarcane detoxication tissue culturing seedling；
C determines the numerous culture intensity of illumination of the expansion of sugarcane detoxication tissue culturing seedling；
D determines temperature needed for the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling；
The time required to E determines the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling.
Preferably, the numerous multiple of expansion described in step B is 3 times.
Preferably, the numerous culture intensity of illumination of expansion described in step C is not less than 3000LX.
Preferably, temperature needed for the numerous culture of expansion described in D steps is 30 DEG C.
Preferably, it is 20 days the time required to the numerous culture of expansion described in E steps, what is obtained is that subculture expands numerous tissue-cultured seedling.
Embodiment 3
A kind of expanding propagation method of sugarcane detoxication tissue culturing seedling, comprises the following steps：
A selects suitable culture medium；
B determines the numerous multiple of the expansion of sugarcane detoxication tissue culturing seedling；
C determines the numerous culture intensity of illumination of the expansion of sugarcane detoxication tissue culturing seedling；
D determines temperature needed for the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling；
The time required to E determines the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling.
Preferably, the numerous multiple of expansion described in step B is 5 times.
Preferably, the intensity of illumination described in step C is not less than 2000LX.
Preferably, temperature needed for the numerous culture of expansion described in D steps is 35 DEG C.
Preferably, it is 30 days the time required to the numerous culture of expansion described in E steps, what is obtained is that subculture expands numerous tissue-cultured seedling.
Claims (5)
1. a kind of expanding propagation method of sugarcane detoxication tissue culturing seedling, it is characterised in that comprise the following steps：
A selects suitable culture medium；
B determines the numerous multiple of the expansion of sugarcane detoxication tissue culturing seedling；
C determines the numerous culture intensity of illumination of the expansion of sugarcane detoxication tissue culturing seedling；
D determines temperature needed for the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling；
The time required to E determines the numerous culture of the expansion of sugarcane detoxication tissue culturing seedling.
2. the expanding propagation method of sugarcane detoxication tissue culturing seedling according to claim 1, it is characterised in that the expansion described in step B is numerous
Multiple is 1-5 times.
3. the expanding propagation method of sugarcane detoxication tissue culturing seedling according to claim 1, it is characterised in that the illumination described in step C
Intensity is not less than 2000LX.
4. the expanding propagation method of sugarcane detoxication tissue culturing seedling according to claim 1, it is characterised in that the expansion described in D steps is numerous
Temperature needed for culture is 25 DEG C -35 DEG C.
5. the expanding propagation method of sugarcane detoxication tissue culturing seedling according to claim 1, it is characterised in that the expansion described in E steps is numerous
It is 15-30 days the time required to culture.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711055843.1A CN107646686A (en) | 2017-11-01 | 2017-11-01 | A kind of expanding propagation method of sugarcane detoxication tissue culturing seedling |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711055843.1A CN107646686A (en) | 2017-11-01 | 2017-11-01 | A kind of expanding propagation method of sugarcane detoxication tissue culturing seedling |
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| CN107646686A true CN107646686A (en) | 2018-02-02 |
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| CN201711055843.1A Pending CN107646686A (en) | 2017-11-01 | 2017-11-01 | A kind of expanding propagation method of sugarcane detoxication tissue culturing seedling |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102405842A (en) * | 2011-10-14 | 2012-04-11 | 福建农林大学 | Open type method for cultivating toxin-free seedlings of sugarcanes |
| CN105010137A (en) * | 2015-06-29 | 2015-11-04 | 大新县科学技术情报研究所 | Saccharum officinarum detoxification seedling propagation method |
| US20160360716A1 (en) * | 2014-08-29 | 2016-12-15 | Pioneer Hi-Bred International, Inc. | Methods and devices for creating doubled haploid embryos using oil matrices |
| CN106818471A (en) * | 2017-01-13 | 2017-06-13 | 广西壮族自治区农业科学院甘蔗研究所 | A kind of method of utilization sugarcane health cultivating seeds high yield sugarcane |
-
2017
- 2017-11-01 CN CN201711055843.1A patent/CN107646686A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102405842A (en) * | 2011-10-14 | 2012-04-11 | 福建农林大学 | Open type method for cultivating toxin-free seedlings of sugarcanes |
| US20160360716A1 (en) * | 2014-08-29 | 2016-12-15 | Pioneer Hi-Bred International, Inc. | Methods and devices for creating doubled haploid embryos using oil matrices |
| CN105010137A (en) * | 2015-06-29 | 2015-11-04 | 大新县科学技术情报研究所 | Saccharum officinarum detoxification seedling propagation method |
| CN106818471A (en) * | 2017-01-13 | 2017-06-13 | 广西壮族自治区农业科学院甘蔗研究所 | A kind of method of utilization sugarcane health cultivating seeds high yield sugarcane |
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Application publication date: 20180202 |
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