CN107637610A - A kind of botanical fungicide for preventing and treating cucumber fusarium axysporum and preparation method thereof - Google Patents
A kind of botanical fungicide for preventing and treating cucumber fusarium axysporum and preparation method thereof Download PDFInfo
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- CN107637610A CN107637610A CN201710896915.9A CN201710896915A CN107637610A CN 107637610 A CN107637610 A CN 107637610A CN 201710896915 A CN201710896915 A CN 201710896915A CN 107637610 A CN107637610 A CN 107637610A
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- fusarium axysporum
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Abstract
The present invention relates to a kind of botanical fungicide for preventing and treating cucumber fusarium axysporum and preparation method thereof, bactericide of the present invention is using plants essential oils such as mustard oil, cinnamon essential oil, Herba Origani Vulgaris quintessence oils as active compound, using natural diatomaceous earth, β-cyclodextrin as auxiliary material, it is prepared through techniques such as synergistic complex, emulsifying, vacuum suction, absorption embeddings.Each component mass content is in bactericide：Mustard oil 10 50%, cinnamon essential oil 5 30%, Herba Origani Vulgaris quintessence oil 5 30%, diatomite 40 60%, maltodextrin 1 10%；The bactericide has good prevention effect to cucumber fusarium axysporum, also has preferable preventive and therapeutic effect to fruits and vegetables root-knot nematode, root rot.
Description
【Technical field】
The present invention relates to disinfectant use in agriculture category, more particularly to be that a kind of plant source for being used to prevent and treat cucumber fusarium axysporum is sterilized
Agent and preparation method thereof.
【Background technology】
Cucumber fusarium axysporum, also known as wilt disease, dead seedling disease, are the Common Diseases in cucumber facility sequential cropping cultivation.Cucumber fusarium axysporum disease
Opportunistic pathogen is pinch outs cucumber specialized form, belongs to Fungi Imperfecti fungi.Cucumber fusarium axysporum is a kind of by soil infection, from root or root neck
Portion invades, and in the entozoic systemic disease of vascular bundle, is one of disease of more difficult preventing and treating on cucumber production, often results in larger damage
Lose.For the germ mainly by soil-borne, spore is in soil, diseased plant residuum and not decomposed farm manure or is attached on seed
Survive the winter, turn into next year primary source of infection, long-distance communications can be carried out by rainwater, irrigation water etc..
At present, before the preventing and treating to cucumber fusarium axysporum is mainly cultivated by using soil sterilants such as bromomethane, formaldehyde
The chemical bactericide such as soil-fumigating sterilization, Bravo that period of disease uses, carbendazim, zineb of phase is prevented and treated, but by
Easily developed immunity to drugs in droop, resistance level rises fast.Cause chemical pesticide largely to use for a long time so that hazard residue and
Environmental pollution equivalent risk becomes increasingly conspicuous.With increasingly strict and Public environmental attitude the reinforcement of environmental legislation, agriculture is avoided or reduced
The use of chemical synthetic pesticide has turned into the inexorable trend of the future of agriculture development, at present, many Chemical Pesticide Varieties in industry production
It is disabled.
Therefore, the problem of being endangered for droop present in current cucumber production, the present invention utilize natural plant
Composition development and the multi-component preventing and treating bactericide of exploitation, not only can effectively control cucumber fusarium axysporum, prevent drug-fast generation, together
When avoid using chemical agent to product safety, the influence of soil environment.Meet the need of current development green ecological agricultural
Ask.
【The content of the invention】
Preventing and treating botanical fungicide of a kind of cucumber fusarium axysporum evil provided by the invention and preparation method thereof, is had by screening
The vegetable-derived components of bactericidal activity, com-parison and analysis difference plant source sterilization component to the bactericidal activity of cucumber fusarium axysporum germ,
It is main to filter out 3 kinds of mustard oil, cinnamon essential oil, Herba Origani Vulgaris quintessence oil compositions that there is good suppression to kill effect to cucumber fusarium axysporum germ
Component, through synergistic complex, diatomite vacuum suction, maltodextrin embedding protection form, it is characterised in that containing biological antibiotic into
Divide 40-60%, adsorbing medium diatomite 40-60%, embedding protective agent maltodextrin 1-10%；It is equal to apply for a patent product sterilization component
For botanical components, the material of any chemical synthesis is being prepared and do not added using process, is being that a kind of production cost is low, environment is friendly
It is good, the cucumber fusarium axysporum pesticide control and preparation method of excellent effect.
Production technology of the present invention comprises the following steps：
1. mixed according to mustard oil 10-50%, cinnamon oil 5-30%, origanum oil 5-30% mass percents, in 5000-12000
Rev/min high speed homogenization under the conditions of homogeneous 5-10 minutes, mix.
2. diatomite is filled in vacuum extractor, makes vacuum be 0.04-0.09MPa using vavuum pump pumping, make
After air discharge in diatomite particle space, mixed as obtained by inlet injection diatomite quality 70-100% above-mentioned steps 1
And essential oil, keep vacuum 0.04-0.09MPa, Static Adsorption 10-20min.
3. by absorption diatomite obtained by above-mentioned 2 step, with waiting diatomite of 0.5-2.0 times of quality to mix, stirred by vulgar
The machine of mixing is sufficiently stirred the absorption diatomite of the decentralized of mixing.
4. adding diatomite quality 5-10% maltodextrin into the gained decentralized diatomite of above-mentioned steps 3, pass through low speed
Mixer 200-1000rpm is sufficiently stirred 5-15min, to realize that the parcel of essential oil embeds.
Specific embodiment
1. cucumber fusarium axysporum pathogeny bacterium plant source Fungicidal substance screens
1.1 plant source Fungicidal substances screen
The inhibitory action of plants essential oil and derived essential oil to cucumber fusarium axysporum opportunistic pathogen is determined using inhibition zone method.As a result show to be sieved
In the vegetable-derived components of choosing, mustard oil is most strong to the bacteriostatic activity of cucumber fusarium axysporum, and antibacterial circle diameter reaches 31.7 ± 0.2mm,
And inhibition zone size is significantly higher than positive control thiram（Valid density is 5mg/mL, similarly hereinafter）（P ＜ 0.05）；Cinnamon essential oil pair
Cucumber fusarium axysporum also has preferable fungistatic effect, and antibacterial circle diameter is 20.7 ± 0.3mm, only secondary mustard oil, bacteriostasis and good fortune
U.S. two-phase is worked as；Herba Origani Vulgaris quintessence oil, carvacrol and Thymol are little to the inhibition gap of cucumber fusarium axysporum, but inhibition zone size
Less than positive control thiram；The effect of the suppression cucumber fusarium axysporum of eugenol and clove oil is poor.
1 different vegetable-derived components of table compare the inhibition zone size of cucumber fusarium axysporum
| Vegetable-derived components | Antibacterial circle diameter（mm） |
| Herba Origani Vulgaris quintessence oil | 15.0±0.4c |
| Carvacrol | 14.5±0.2c |
| Clove oil | 8.8±0.1d |
| Eugenol | 10.0±0.1d |
| Cinnamon essential oil | 20.7±0.3b |
| Mustard oil | 31.7±0.2a |
| Cumin essential oil | 6.0±0e |
| Cumal | 6.0±0e |
| Thymol | 14.5±0.1c |
| Positive control | 20.0±0.3b |
| Negative control | 6.0±0e |
Note：The identical difference that represents of letter is not notable in table, and difference represents significant difference（P ＜ 0.05）
1.2 plant source sterilization component minimum bactericidal concentrations compare：
By on the plant source sterilization component 0.1ml culture transferrings of gradient dilution to the nutrient agar plate of not drug containing, it is coated with sterilizing
Device touches decoction coating uniformly, and pathogen is placed in 28 DEG C of incubators and cultivates 48h, and plate coating observation whether there is fungus life
Long, 99.9% that reduction primary colony is counted using in plate culture dish is used as minimum bactericidal concentration).As a result show, mustard oil is to Huang
The minimum bactericidal concentration of cucurbit wilt is minimum, is 0.049 μ L/mL.Next to that cinnamon essential oil, the μ L/ of minimum bactericidal concentration value 0.098
mL.The minimum bactericidal concentration value of remaining bacteriostatic agent is larger, and the minimum bactericidal concentration of mustard oil and cinnamon essential oil is less than 0.1 μ L/
ML, illustrate that mustard oil and cinnamon oil have preferable bactericidal effect to cucumber fusarium axysporum under low concentration.
Minimum bactericidal concentration of the plant source Fungicidal substance of table 2 to cucumber fusarium axysporum germ
| Plant source Fungicidal substance | Minimum bactericidal concentration (μ L/mL) |
| Mustard oil | 0.049 |
| Cinnamon essential oil | 0.098 |
| Clove oil | 3.125 |
| Eugenol | 1.563 |
| Thymol | 0.391 |
| Herba Origani Vulgaris quintessence oil | 0.391 |
| Carvacrol | 0.391 |
| Positive control | 0.049 |
Note：Thymol is solid crystal, and concentration is expressed as mg/mL
Positive control：Thiram（Valid density, mg/mL）
2. a kind of botanical fungicide for being used to prevent and treat cucumber fusarium axysporum prepares example
Mustard oil 400g, origanum oil 100g, cinnamon oil 100g are weighed respectively, under the conditions of 10000rpm high speed homogenization successively
Add homogeneous 10 minutes, mix, it is standby.Weigh 300g diatomite to be filled in vacuum extractor, make vacuum using vavuum pump pumping
Spend for 0.06MPa, pass through inlet and inject the above-mentioned mixing essential oil of diatomite quality, keep vacuum 0.6MPa, Static Adsorption
20min.The diatomite for taking out absorption essential oil is mixed with the diatomite of 600 mass, and mixing is sufficiently stirred by vulgar mixer
The absorption diatomite of decentralized.The maltodextrin of diatomite quality 80 is added in decentralized diatomite, passes through low-speed mixer
200rpm is sufficiently stirred 15min.
3. cucumber substrate culture application example
Before matrix fills basin or upper facility cultivation bed, matrix per ton applies 50g bactericide of the present invention, fully mixes for cucumber substrate culture
After even, 20kg water is sprayed to stromal surface with sprayer, prevents active component from volatilizing with plastic cloth covering, plastics is thrown off after 24h
Cloth enters the potted plant training of luggage.Using《Pesticide field efficacy medicine test criterion》Specified in method, plant source of the embodiment of the present invention is killed
The field efficacy of microbial inoculum is tested, and usage amount is the grams of bactericide used in matrix per ton.It the results are shown in Table 3.
The different amounts botanical fungicide of table 3 is to 3 years matrix prevention effects of continuous cropping
4. cucumber in greenhouse cultivating soil application example
With reference to turning over before field planting, every mu is spread fertilizer over the fields 800g Soil tillages and enters soil, after making bactericide, takes covering with ground sheeting or vexed
Canopy is handled 2-3 days, and facility cultivation can be carried out after throwing off mulch film or ventilation.
Using《Pesticide field efficacy medicine test criterion》Specified in method, to the field of botanical fungicide of the embodiment of the present invention
Between drug effect tested, usage amount be bactericide used in mu soil kilogram number.It the results are shown in Table 2.
The different amounts botanical fungicide of table 4 is to 3 years matrix prevention effects of continuous cropping
Claims (8)
- A kind of 1. botanical fungicide for preventing and treating cucumber fusarium axysporum, it is characterised in that it be pulvis that agent is killed in the suppression, its raw material form and Mass percentage content is：Mustard oil 10-50%, cinnamon essential oil 5-30%, Herba Origani Vulgaris quintessence oil 5-30%, diatomite 40-60%, malt paste Smart 1-10%.
- A kind of 2. botanical fungicide for preventing and treating cucumber fusarium axysporum as claimed in claim 1, it is characterised in that described raw material mustard Isosulfocyanate compound mass content is 75-98% in last oil.
- A kind of 3. botanical fungicide for preventing and treating cucumber fusarium axysporum as described in right 1, it is characterised in that described raw material cinnamon spirit The mass content of cinnamic acid is 58-76% in oil.
- A kind of 4. botanical fungicide for preventing and treating cucumber fusarium axysporum as described in right 1, it is characterised in that described active compound wild marjoram essence The mass content of carvacrol is 40-65% in oil.
- 5. as described in right 1, a kind of botanical fungicide for preventing and treating cucumber fusarium axysporum, it is characterised in that malt is pasted in the dispensing The hydrolysis DE values of essence are in 5-15%.
- 6. a kind of botanical fungicide and preparation method for preventing and treating cucumber fusarium axysporum, its feature is according to institute described in claim 1-3 The botanical fungicide raw material composition and mass percent stated prepare raw material, further comprising the steps of：A. mixed according to mustard oil 10-50%, cinnamon oil 5-30%, Herba Origani Vulgaris quintessence oil 5-30% mass percents, in 5000- Add high speed homogenization 5-10 minutes under the conditions of 12000 revs/min of high speed homogenization successively, mix；B. diatomite is filled in vacuum extractor, makes vacuum be 0.04-0.09MPa using vavuum pump pumping, pass through note Essential oil is mixed obtained by entrance injection diatomite quality 70-100% above-mentioned a, keeps vacuum 0.04-0.09MPa, Static Adsorption 10-20min；C. by absorption diatomite obtained by above-mentioned b step, mix with the diatomite of 0.5-2.0 times of quality, filled by low-speed mixer Divide the absorption diatomite of the decentralized stirred and evenly mixed；D. diatomite quality 5-10% maltodextrin is added into decentralized diatomite obtained by above-mentioned step c, passes through stirring at low speed Machine is sufficiently stirred 5-15min, to realize that the parcel of essential oil embeds.
- 7. as described in right 6, a kind of botanical fungicide and preparation method for preventing and treating cucumber fusarium axysporum, it is characterised in that its product In production process, vacuum plant used is both ends open as shown below and can realize the vacuum tube or vacuum of essential oil vacuum injection Tank.
- 8. as described in right 6, a kind of botanical fungicide and preparation method for preventing and treating cucumber fusarium axysporum, it is characterised in that its product In production process, the vacuum sucking operation process of essential oil is：After diatomite loads, it is 0.04- that vavuum pump, which is pumped to pressure inside the tank, During 0.09MPa, vavuum pump and bleeding point valve are closed（Valve B in schematic diagram）, pressure inside the tank is kept, passes through material inlet valve（Show Valve A in intention）After quantitative essential oil is injected into tank, material inlet valve is closed, keeps 0.04-0.09MPa vacuum 10-20min, Carry out the vacuum suction of essential oil.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115500363A (en) * | 2022-09-20 | 2022-12-23 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Mildew-proof composite with synergistic effect and preparation method and application thereof |
Citations (3)
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| CN102805114A (en) * | 2012-08-30 | 2012-12-05 | 白静 | Botanical natural fungicide for fungus and preparation method thereof |
| CN103891752A (en) * | 2014-04-11 | 2014-07-02 | 青岛农业大学 | Sterilization mixed preparation containing mustard essential oil and allicin and application |
| WO2015044039A1 (en) * | 2013-09-30 | 2015-04-02 | Basf Se | Synergistic fungicidal compositions containing khc03 |
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2017
- 2017-09-28 CN CN201710896915.9A patent/CN107637610B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805114A (en) * | 2012-08-30 | 2012-12-05 | 白静 | Botanical natural fungicide for fungus and preparation method thereof |
| WO2015044039A1 (en) * | 2013-09-30 | 2015-04-02 | Basf Se | Synergistic fungicidal compositions containing khc03 |
| CN103891752A (en) * | 2014-04-11 | 2014-07-02 | 青岛农业大学 | Sterilization mixed preparation containing mustard essential oil and allicin and application |
Non-Patent Citations (2)
| Title |
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| M.C. MANGANYI等: "Antimicrobial activities of selected essential oils against Fusarium oxysporum isolates and their biofilms", 《SOUTH AFRICAN JOURNAL OF BOTANY》 * |
| 贾会玲等: "9种植物精油及精油单体成分对黄瓜枯萎病的抑菌活性", 《安徽农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115500363A (en) * | 2022-09-20 | 2022-12-23 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Mildew-proof composite with synergistic effect and preparation method and application thereof |
| CN115500363B (en) * | 2022-09-20 | 2023-06-27 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Mildew-proof composite composition with synergistic effect and preparation method and application thereof |
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