CN107637525A - A kind of tissue culture propagation and purposes of Yunnan Mao Xu Cao - Google Patents
A kind of tissue culture propagation and purposes of Yunnan Mao Xu Cao Download PDFInfo
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- CN107637525A CN107637525A CN201711161780.8A CN201711161780A CN107637525A CN 107637525 A CN107637525 A CN 107637525A CN 201711161780 A CN201711161780 A CN 201711161780A CN 107637525 A CN107637525 A CN 107637525A
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Abstract
The present invention relates to a kind of Yunnan Mao Xu Cao tissue culture propagation and purposes.Utilize tissue cultivation rapid breeding method, the wild Mao Xu Cao blade of In Xishuangbanna of Yunnan is induced to produce callus, and a large amount of sterile tissue-cultured seedling are produced after differential medium culture, proliferated culture medium culture, root media culture, utilize the blade of sterile tissue-cultured seedling, continue callus induction, Proliferation, Differentiation, take root, a large amount of sterile tissue-cultured seedling are obtained, experienced after seedling migrates to 2 through uncapping：1（Vermiculite：Soil）In substrate soil, grow 15 20 days, water spray periodically management daily, after slow seedling grows fine, field-transplanting is transplanted, survival rate is up to 95%, pass through extensive tissue culture propagation Mao Xu Cao, Mao Xu Cao cultivated area can be made to expand hundred times, the demand in market is met, promoted cat's whisker grassland industry quickly to develop.
Description
Technical field
The invention belongs to biotechnology engineering field, and in particular to a kind of tissue culture propagation of Yunnan Mao Xu Cao and
Purposes.
Background technology
Mao Xu Cao is Labiatae kidney Camellia herbaceos perennial.The torrid zone that Mao Xu Cao originates in Southeast Asia to Australia is sub-
Torrid areas, the ground such as Guangdong, Hainan, south of Yunnan, south Guangxi, Taiwan and Fujian are distributed mainly in China.South China
There is growth in portion area, and the north is more rare, and Mao Xu Cao is to acute and chronic nephritis, cystitis, lithangiuria, rheumatic joint of lower extremity
Inflammation etc. has special efficacy, and has anti-inflammation, antiallergy and anti-chronic renal failure concurrently, the function such as increase kidney,liver,spleen CBF,
Folks of china has long history with its disease-treatment and health-care.It is increasingly exhausted yet with the Mao Xu Cao resources of natural crude drugs, but due to
Mao Xu Cao fruiting rate is low, and seed longeivity only has 15d, and germination percentage is only 40%, and traditional mode of production modes of reproduction is difficult to meet market need
Ask.For Mao Xu Cao as a kind of plant with huge DEVELOPMENT PROSPECT, the report of tissue-culturing rapid propagation is very limited, not yet establishes generally acknowledge at present
Complete tissue cultivating system, therefore the Vitro Quick Reproduction of Mao Xu Cao turn into amount reproduction Mao Xu Cao seedling effective way.
The content of the invention
It is an object of the invention to provide a kind of tissue culture propagation of Yunnan Mao Xu Cao.Utilize the method for the present invention
Large-scale breeding is carried out to Yunnan Mao Xu Cao, low to solve Yunnan south Mao Xu Cao fruiting rate, seed longeivity is short, and germination percentage is low, day
The problems such as difficult and Yunnan cat's whisker grass resource is increasingly deficient is bred under right state, realize Mao Xu Cao Mao Xu Cao it is in vitro quick
Breed and meet the needs of extensive artificial growth.
In order to realize the above-mentioned purpose of the present invention, the invention provides following technical scheme：
A kind of tissue culture propagation of Yunnan Mao Xu Cao, it is characterised in that carried out by the steps：
（1) culture medium is prepared：The minimal medium used in method and every liter of other each stage tissue culture culture medium each components
Content is：
Minimal medium：With MS culture mediums, white sugar 25-40g/L, agar 6-8g/L are added, is adjusted with 2% sodium hydroxide or 1N hydrochloric acid
Save pH5.8-6.0；
Callus inducing medium：6BA 1.5-4mg/L and NAA 0.1-1mg/L are added in MS；
Proliferated culture medium：6BA0.1-0.5mg/L and NAA .01-2.0mg/L are added in MS；
Differential medium：6-BA 0.25-0.5mg/L+NAA 0.1mg-2mg/L are added in MS；
Root media：NAA 2.0-4.0mg/L are added in MS；
After preparing each stage culture medium, pH5.8-6.0 is adjusted with 2% sodium hydroxide or 1N hydrochloric acid；It is divided in tissue culture flasks,
121 DEG C of autoclaving 20min, room temperature are cooled to solidification；
（2）The plantation of Mao Xu Cao and the sterilization of explant：From the Mao Xu Cao branch of In Xishuangbanna of Yunnan field acquisition, plant in 2：
1 Nutrition Soil：Vermiculite（v/v）In flowerpot, wait to grow new young leaflet tablet after 15 days standby；Clip 5-10 piece blades, are put into dry
In net blake bottle, distilled water and white cat liquid detergent are added, shakes and washes on shaking table, 100 turns/min30min；Then distilled water is used
Cleaning solution is cleaned, removes excessive moisture；In super-clean bench, washing blade 30-60s is jiggled with 75% ethanol, will with sterilized water
Ethanol is cleaned；Blade is put into 0.1%（w/w）In mercuric chloride solution, jiggle, wash 8min；With sterilized water washing blade 4
It is secondary, it is positioned over stand-by on the filter paper in the clean culture dish that sterilized;
（3）The induction of Mao Xu Cao callus：In super-clean bench, the explant blade of sanitized is picked up with tweezers, leaf
Piece damaged portion is cut off；Leaf margin is slightly wiped out with scissors, creates blade wound, then blade is cut into 0.5cm × 0.5cm sizes；
Blade will be sheared with tweezers to be respectively put into MS in addition 6BA 1.5-4mg/L and NAA 0.1-1mg/L calli induction medias,
The culture medium prepares in advance and the 20min that sterilized at 121 DEG C, the culture medium cooled down at room temperature, every bottle of 3-5 piece, will cultivate bottleneck
Calcination sterilizes and covers bottle cap；Blake bottle is put into dark place light culture 2-4 weeks, 24 DEG C of temperature；
（4）The propagation of Mao Xu Cao callus：Original cuiture 30 days, in super-clean bench, with the tweezers of sterilizing by callus lines
Pressed from both sides out from old blake bottle, callus lines are larger, cut with blade, make every piece tissue size be 0.5cm ×
0.5cm, access on fresh MS+6-BA 0.10mg-0.5mg/L+NAA 1mg-2mg/L culture mediums, 121 DEG C of autoclavings
20min, carry out squamous subculture；Mao Xu Cao callus after switching is placed under light and cultivated, intensity of illumination 3000lux, temperature
24 DEG C, illumination 16h, dark 8h；
（5) differentiation of Mao Xu Cao callus：After step 3 is cultivated 30 days, callus has carried out substantial amounts of propagation, will breed
Callus move to MS+6-BA 0.25mg/L (or 0.5mg/L)+NAA 0.1mg/L, 121 DEG C of autoclaving 20min differentiation
Make its differentiation budding on culture medium；
（6) culture of rootage：The clump bud block for breeding formation is cut into simple bud one by one, moves in root media MS and adds
On NAA2mg-4mg/L culture mediums, 121 DEG C of autoclaving 20min, every bottle of culture medium puts 3-5 simple bud, in root media
Carry out culture of rootage 15-20 days；
（7）Practice seedling：Carry out uncapping experienced seedling 3-5 days when the seedling in root media, root long to 2 centimeter lengths, radical 3-5 bars,
Survival rate is higher when now root grows vigorous；
（8）Transplanting：Practice seedling after 3-5 days, seedling is taken out from tissue culture bottle, after washing twice with clear water, then soak 20min with carbendazim and move
Plant in matrix, in greenhouse culture into.
Seedling Yunnan Mao Xu Cao tissue culture propagating technology, comprises the following steps：
Mao Xu Cao quick breeding method for tissue culture in Yunnan of the present invention in further detail the step of be：
（1) culture medium is prepared, every liter of content of minimal medium and other each stage tissue culture culture medium each components is：
Minimal medium：With MS culture mediums, white sugar 25-40g/L, agar 6-8g/L are added, is adjusted with 2% sodium hydroxide or 1N hydrochloric acid
Save pH5.8-6.0；
Callus inducing medium：6BA 1.5-4mg/L and NAA 0.1-1mg/L are added in MS；
Proliferated culture medium：6BA0.1-0.5mg/L and NAA .01-2.0mg/L are added in MS；
Differential medium：6-BA 0.25-0.5mg/L are added in MS)+NAA 0.1mg-2mg/L；
Root media：NAA 2.0-4.0mg/L are added in MS；
After preparing each stage culture medium, pH5.8-6.0 is adjusted with 2% sodium hydroxide or 1N hydrochloric acid；It is divided in tissue culture flasks,
121 DEG C of autoclaving 20min, room temperature are cooled to solidification.
（2）The plantation of field Mao Xu Cao material and the sterilization of blade：From the Mao Xu Cao branch of In Xishuangbanna of Yunnan field acquisition
Bar, plant in flowerpot, wait to grow new young leaflet tablet standby.Clip 5-10 piece blades, are put into clean blake bottle, add
Enter appropriate distilled water and white cat liquid detergent, shaken on shaking table and wash 30min；Liquid detergent is cleaned with distilled water after washing, it is unnecessary to remove
Moisture；In super-clean bench, jiggled with 70-75% ethanol, washing blade 30-60s, cleaned ethanol with sterilized water；By blade
It is put into 0.1%-1% mercuric chloride solutions, sterilizes 8-10min；With sterilized water washing blade 4-6 times, it is positioned in clean culture dish
Filter paper on.
（3）Scissors tweezers are placed in above alcolhol burner and fully burnt in super-clean bench by the induction of Yunnan Mao Xu Cao callus
Sterilizing is burnt, is placed on clean culture dish and cools down；The above-mentioned explant blade disinfected is picked up with tweezers, is damaged blade with scissors
Traumatic part sub-cut is gone；Again leaf margin is slightly wiped out with scissors, artificial creation's blade wound, then blade is cut into appropriately sized（Typically
5mmx5mm）；Blade, which will be sheared, with tweezers is respectively put into addition 6BA 0.25-4.0mg/L and NAA 0.1- in the MS prepared in advance
In 2.0mg/L calli induction media, every bottle of 3-5 piece, culture bottleneck calcination is sterilized and covers bottle cap；Blake bottle is put into
Under the conditions of 25 DEG C, dark place light culture 2-4 weeks.
（4）The propagation of Mao Xu Cao callus：Original cuiture 30d or so, in super-clean bench, with the tweezers of sterilizing by callus
Tissue block presss from both sides out from old blake bottle, and callus lines are larger, is cut with blade, make every piece tissue size be about
0.5cm × 0.5cm, access on fresh MS+6-BA 0.10mg-0.5mg/L+NAA 1mg-2mg/L culture mediums, carry out after
It is commissioned to train foster.Mao Xu Cao callus after switching is placed under light and cultivated, 23-25 DEG C of temperature, intensity of illumination 2000-
3000lux, light application time 16h, dark culturing/8h, culture 30d or so.
（5）The differentiation of Mao Xu Cao callus：By step 3 induced synthesis callus, hand is used on sterile super-clean bench
Callus is cut into 0.5cm or so size by art knife, moves to MS+6-BA 0.25mg/L (or 0.5mg/L)+NAA 0.1mg/L
On differential medium, make its differentiation budding.
（6）The culture of rootage of Mao Xu Cao bud：The clump bud block for breeding formation is cut into simple bud one by one, moves to root media
Added in MS on NAA2-4mg/L culture mediums, every bottle of culture medium puts 3-5 simple bud, and culture of rootage is carried out in root media
15-20d or so；
（7）Practice seedling：The experienced seedling 3-5d that uncaps is carried out when the seedling in root media, root long to 2 centimeter lengths, radical 3-5 bars, this
Shi Genzheng growths are vigorous, and survival rate is higher after transplanting.
（8）Transplanting：Practice seedling after 3-5 days, seedling is taken out from tissue culture bottle, twice is washed with clear water and cleans culture medium, then with more
Bacterium spirit immersion 10-20min is transplanted into Nutrition Soil：Vermiculite 2：In the matrix of 1 mixing, in greenhouse after slow seedling 15-20d, carry out big
Field is transplanted, survival rate 95%.
The present invention further discloses the tissue culture propagation of Yunnan Mao Xu Cao for the in vitro quick of Mao Xu Cao
The application of proliferation.The present invention is to carry out extensive quick breeding Mao Xu Cao seedling using the spire of wild Yunnan Mao Xu Cao,
Method is to carry out callus induction by explant of the Yunnan Mao Xu Cao blade of field acquisition, and by breaking up, breeding, taking root makes
It regenerates plant.The callus of the present invention produces the differentiation rate of bud and root up to more than 90%, and transplantation of seedlings is survived up to more than 95%.
By extensive tissue culture propagation Mao Xu Cao, Mao Xu Cao cultivated area can be made to expand hundred times, meet the demand in market, promoted
Cat's whisker grassland industry quickly develops.
Brief description of the drawings
The cultivation of the wild material of Fig. 1 Yunnan Mao Xu Cao indoors；
Fig. 2 Yunnan Mao Xu Cao tender leaf dedifferentiation, propagation in initial proliferated culture medium form callus；
Induction differentiation culture of Fig. 3 Yunnan Mao Xu Cao Callus of Leaf in differential medium forms adventitious bud and seedling；
Root induction differentiation culture of Fig. 4 Yunnan Mao Xu Cao seedling in root induction culture medium；
The seedling that Fig. 5 Yunnan Mao Xu Cao seedling grows in root media；
Fig. 6 Yunnan Mao Xu Cao seedling practices seedling and transplanting.
Embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
The various changes carried out on the premise of invention spirit and scope to the material component in these embodiments and dosage or change
Belong to protection scope of the present invention.Raw materials used and reagent of the invention is commercially available.
Raw material sources：
Embodiment 1
（1）The plantation of Mao Xu Cao and the sterilization of explant from the Mao Xu Cao branch of In Xishuangbanna of Yunnan field acquisition, plant in containing
Have 2：1 Nutrition Soil：In vermiculite flowerpot, wait to grow new young leaflet tablet after 15 days standby（Fig. 1）.10 blades of clip, are put into
In clean blake bottle, appropriate distilled water and white cat liquid detergent are added, shakes and washes on shaking table（100 turns/min）30min；Then use
Distilled water cleans cleaning solution, removes excessive moisture；In super-clean bench, washing blade 45s is jiggled with 75% ethanol, use is sterile
Water cleans ethanol；Blade is put into 0.1% mercuric chloride solution, jiggled, washs 8min；With sterilized water washing blade 4
It is secondary, it is positioned over stand-by on the filter paper in the clean culture dish that sterilized.
（2）The induction of Mao Xu Cao callus：In super-clean bench, scissors, tweezers are placed in abundant calcination above alcolhol burner
Sterilizing, is placed on clean culture dish and cools down；The explant blade of sanitized is picked up with tweezers, blade injury part is cut
Go；Leaf margin is slightly wiped out with scissors, creates blade wound, then blade is cut into 0.5cm × 0.5cm sizes；It will be sheared with tweezers
Blade is respectively put into MS and added in 6BA 1.5mg/L and NAA 0.1mg/L calli induction medias（Fig. 2）, the culture medium thing
First prepare and the 20min that sterilized at 121 DEG C, the culture medium cooled down at room temperature.4 every bottle, culture bottleneck calcination is sterilized and covered
Good bottle cap；Blake bottle is put into dark place light culture 4 weeks, 24 DEG C of temperature.
（3）The propagation of Mao Xu Cao callus：Original cuiture 30 days or so, will be more with the tweezers of sterilizing in super-clean bench
Injured tissue block presss from both sides out from old blake bottle, and callus lines are larger, is cut with blade, make every piece tissue size be about
0.5cm×0.5cm（Fig. 3）, access on fresh MS+6-BA 0.5mg/L+NAA 1mg/L culture mediums（121 DEG C of autoclavings
20min）, carry out squamous subculture.Mao Xu Cao callus after switching is placed under light and cultivated, intensity of illumination 3000lux, temperature
24 DEG C of degree, illumination 16h, dark 8h.
（4) differentiation of Mao Xu Cao callus：After step 3 is cultivated 30 days, callus has carried out substantial amounts of propagation, will
The callus of propagation moves to MS+6-BA 0.25mg/L (or 0.5mg/L)+NAA 0.1mg/L（121 DEG C of autoclaving 20min）
Make its differentiation budding on differential medium（Fig. 4）.
（5) culture of rootage：The clump bud block for breeding formation is cut into simple bud one by one, moves in root media MS and adds
On NAA2mg-4mg/L culture mediums（121 DEG C of autoclaving 20min）, every bottle of culture medium puts 3-5 simple bud, in root media
Carry out culture of rootage 20 days（Fig. 5）；
（6）Practice seedling：Carry out uncapping experienced seedling 4 days when the seedling in root media, when root long to 2 centimeter lengths, radical 5, now
Survival rate is higher when root grows vigorous（Fig. 6）.
（7）Transplanting：Practice seedling after 4 days, seedling is taken out from tissue culture bottle, after washing twice with clear water, then with carbendazim soak 20min
It is transplanted into matrix, is cultured to be a seedling in greenhouse（Fig. 6）.More than 90% seedling transplanted all has survived as we can see from the figure
And growth is vigorous.
Embodiment 2
（1）The induction of callus：By step（5）The seedling of culture of rootage, under sterile super-clean bench, the blade that will grow out
0.3cm × 0.3cm sizes are cut into, will shear blade with tweezers is respectively put into addition 6BA 2.0mg/L and NAA 0.3mg/L in MS
In calli induction media（Fig. 2）, every bottle of 3-5 piece, culture bottleneck calcination is sterilized and covers bottle cap；Blake bottle is put into dark place
Light culture 20d, 24 DEG C of temperature.
（2）The propagation of Mao Xu Cao callus：Original cuiture 30 days or so, will be more with the tweezers of sterilizing in super-clean bench
Injured tissue block presss from both sides out from old blake bottle, and callus lines are larger, is cut with blade, make every piece tissue size be about
0.5cm×0.5cm（Fig. 3）, access on fresh MS+6-BA 1mg/L+NAA 0.5mg/L culture mediums（121 DEG C of autoclavings
20min）, carry out squamous subculture.Mao Xu Cao callus after switching is placed under light and cultivated, intensity of illumination 3000lux, temperature
24 DEG C of degree, illumination 16h, dark 8h.
（3) differentiation of Mao Xu Cao callus：After step 3 is cultivated 30 days, callus has carried out substantial amounts of propagation, will
The callus of propagation moves to MS+6-BA 0.5mg/L+NAA 0.5mg/L（121 DEG C of autoclaving 20min）On differential medium
Make its differentiation budding（Fig. 4）.
（4) culture of rootage：The clump bud block for breeding formation is cut into simple bud one by one, moves in root media MS and adds
On NAA3mg/L culture mediums（121 DEG C of autoclaving 20min）, every bottle of culture medium puts 3 simple buds, given birth in root media
Root culture 20 days or so（Fig. 5）；
（5）Practice seedling：The experienced seedling 5d that uncaps is carried out when the seedling in root media, root long to 2 centimeter lengths, radical 3-5 bars, now
Survival rate is higher when root grows vigorous（Fig. 6）, seedling growth is vigorous as we can see from the figure, and death rate is relatively low, completely may be used
Produced with being transplanted to crop field.
Claims (2)
1. a kind of tissue culture propagation of Yunnan Mao Xu Cao, it is characterised in that carried out by the steps：
（1) culture medium is prepared：The minimal medium used in method and every liter of other each stage tissue culture culture medium each components
Content is：
Minimal medium：With MS culture mediums, white sugar 25-40g/L, agar 6-8g/L are added, is adjusted with 2% sodium hydroxide or 1N hydrochloric acid
Save pH5.8-6.0；
Callus inducing medium：6BA 1.5-4mg/L and NAA 0.1-1mg/L are added in MS；
Proliferated culture medium：6BA0.1-0.5mg/L and NAA .01-2.0mg/L are added in MS；
Differential medium：6-BA 0.25-0.5mg/L+NAA 0.1mg-2mg/L are added in MS；
Root media：NAA 2.0-4.0mg/L are added in MS；
After preparing each stage culture medium, pH5.8-6.0 is adjusted with 2% sodium hydroxide or 1N hydrochloric acid；It is divided in tissue culture flasks,
121 DEG C of autoclaving 20min, room temperature are cooled to solidification；
（2）The plantation of Mao Xu Cao and the sterilization of explant：From the Mao Xu Cao branch of In Xishuangbanna of Yunnan field acquisition, plant in 2：
1 Nutrition Soil：Vermiculite（v/v）In flowerpot, wait to grow new young leaflet tablet after 15 days standby；Clip 5-10 piece blades, are put into dry
In net blake bottle, distilled water and white cat liquid detergent are added, shakes and washes on shaking table, 100 turns/min30min；Then distilled water is used
Cleaning solution is cleaned, removes excessive moisture；In super-clean bench, washing blade 30-60s is jiggled with 75% ethanol, will with sterilized water
Ethanol is cleaned；Blade is put into 0.1%（w/w）In mercuric chloride solution, jiggle, wash 8min；With sterilized water washing blade 4
It is secondary, it is positioned over stand-by on the filter paper in the clean culture dish that sterilized;
（3）The induction of Mao Xu Cao callus：In super-clean bench, the explant blade of sanitized is picked up with tweezers, leaf
Piece damaged portion is cut off；Leaf margin is slightly wiped out with scissors, creates blade wound, then blade is cut into 0.5cm × 0.5cm sizes；
Blade will be sheared with tweezers to be respectively put into MS in addition 6BA 1.5-4mg/L and NAA 0.1-1mg/L calli induction medias,
The culture medium prepares in advance and the 20min that sterilized at 121 DEG C, the culture medium cooled down at room temperature, every bottle of 3-5 piece, will cultivate bottleneck
Calcination sterilizes and covers bottle cap；Blake bottle is put into dark place light culture 2-4 weeks, 24 DEG C of temperature；
（4）The propagation of Mao Xu Cao callus：Original cuiture 30 days, in super-clean bench, with the tweezers of sterilizing by callus lines
Pressed from both sides out from old blake bottle, callus lines are larger, cut with blade, make every piece tissue size be 0.5cm ×
0.5cm, access on fresh MS+6-BA 0.10mg-0.5mg/L+NAA 1mg-2mg/L culture mediums, 121 DEG C of autoclavings
20min, carry out squamous subculture；Mao Xu Cao callus after switching is placed under light and cultivated, intensity of illumination 3000lux, temperature
24 DEG C, illumination 16h, dark 8h；
（5) differentiation of Mao Xu Cao callus：After step 3 is cultivated 30 days, callus has carried out substantial amounts of propagation, will breed
Callus move to MS+6-BA 0.25mg/L (or 0.5mg/L)+NAA 0.1mg/L, 121 DEG C of autoclaving 20min differentiation
Make its differentiation budding on culture medium；
（6) culture of rootage：The clump bud block for breeding formation is cut into simple bud one by one, moves in root media MS and adds
On NAA2mg-4mg/L culture mediums, 121 DEG C of autoclaving 20min, every bottle of culture medium puts 3-5 simple bud, in root media
Carry out culture of rootage 15-20 days；
（7）Practice seedling：Carry out uncapping experienced seedling 3-5 days when the seedling in root media, root long to 2 centimeter lengths, radical 3-5 bars,
Survival rate is higher when now root grows vigorous；
（8）Transplanting：Practice seedling after 3-5 days, seedling is taken out from tissue culture bottle, after washing twice with clear water, then soak 20min with carbendazim and move
Plant in matrix, be cultured to be a seedling in greenhouse.
2. the tissue culture propagation of the Yunnan Mao Xu Cao described in claim 1 is in the Vitro Quick Reproduction side for Mao Xu Cao
The application in face.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109496706A (en) * | 2018-12-06 | 2019-03-22 | 广西壮族自治区亚热带作物研究所 | A kind of sterile brachyplast of Mao Xu Cao quickly breed before explant preprocess method |
| CN115024225A (en) * | 2022-07-06 | 2022-09-09 | 天津博奥聚能生物科技有限公司 | Culture method of cold-resistant orthosiphon aristatus |
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| KR101053986B1 (en) * | 2009-04-30 | 2011-08-04 | 주식회사 그래미 | Production method of callus tissue of Hong Kyung-cheon |
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2017
- 2017-11-21 CN CN201711161780.8A patent/CN107637525A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101053986B1 (en) * | 2009-04-30 | 2011-08-04 | 주식회사 그래미 | Production method of callus tissue of Hong Kyung-cheon |
Non-Patent Citations (1)
| Title |
|---|
| 李任珠等: "组织培养快速繁殖肾茶的研究", 《海南大学学报自然科学版》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109496706A (en) * | 2018-12-06 | 2019-03-22 | 广西壮族自治区亚热带作物研究所 | A kind of sterile brachyplast of Mao Xu Cao quickly breed before explant preprocess method |
| CN115024225A (en) * | 2022-07-06 | 2022-09-09 | 天津博奥聚能生物科技有限公司 | Culture method of cold-resistant orthosiphon aristatus |
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