CN107636005B - 在色谱过程中利用碱洗涤去除杂质 - Google Patents

在色谱过程中利用碱洗涤去除杂质 Download PDF

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Publication number
CN107636005B
CN107636005B CN201680015033.0A CN201680015033A CN107636005B CN 107636005 B CN107636005 B CN 107636005B CN 201680015033 A CN201680015033 A CN 201680015033A CN 107636005 B CN107636005 B CN 107636005B
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chromatography
protein
interest
wash solution
antibody
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Chinese (zh)
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CN107636005A (zh
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J·王
N·E·贾菲
K·帕特尔
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Bristol Myers Squibb Co
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Bristol Myers Squibb Co
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN201680015033.0A 2015-03-13 2016-03-11 在色谱过程中利用碱洗涤去除杂质 Active CN107636005B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562132974P 2015-03-13 2015-03-13
US62/132,974 2015-03-13
PCT/US2016/021984 WO2016149088A1 (en) 2015-03-13 2016-03-11 Use of alkaline washes during chromatography to remove impurities

Publications (2)

Publication Number Publication Date
CN107636005A CN107636005A (zh) 2018-01-26
CN107636005B true CN107636005B (zh) 2021-07-16

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Country Status (13)

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US (2) US10695693B2 (https=)
EP (2) EP3268100A1 (https=)
JP (1) JP6807859B2 (https=)
KR (1) KR102490956B1 (https=)
CN (1) CN107636005B (https=)
AU (2) AU2016233557B2 (https=)
BR (1) BR112017018365A2 (https=)
CA (1) CA2978095C (https=)
EA (1) EA035635B1 (https=)
IL (1) IL254372B (https=)
MX (1) MX395730B (https=)
SG (2) SG11201707330RA (https=)
WO (1) WO2016149088A1 (https=)

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JP2022514122A (ja) * 2018-10-31 2022-02-09 リジェネロン・ファーマシューティカルズ・インコーポレイテッド タンパク質を同定及び定量化する方法及びシステム
WO2020125757A1 (en) * 2018-12-21 2020-06-25 Wuxi Biologics (Shanghai) Co., Ltd. A method for improving aggregate removal by protein a chromatography
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Also Published As

Publication number Publication date
AU2016233557A1 (en) 2017-11-02
MX395730B (es) 2025-03-25
EA035635B1 (ru) 2020-07-17
IL254372B (en) 2022-05-01
CA2978095A1 (en) 2016-09-22
US10695693B2 (en) 2020-06-30
AU2021221870A1 (en) 2021-09-23
MX2017011121A (es) 2017-11-28
US20200282332A1 (en) 2020-09-10
KR20170125948A (ko) 2017-11-15
SG10201908027QA (en) 2019-10-30
JP2018510867A (ja) 2018-04-19
US12157073B2 (en) 2024-12-03
IL254372A0 (en) 2017-11-30
CN107636005A (zh) 2018-01-26
EP4194071A1 (en) 2023-06-14
EA201792012A1 (ru) 2018-01-31
JP6807859B2 (ja) 2021-01-06
BR112017018365A2 (pt) 2018-04-10
US20180078876A1 (en) 2018-03-22
AU2016233557B2 (en) 2021-06-24
KR102490956B1 (ko) 2023-01-19
CA2978095C (en) 2022-02-15
EP3268100A1 (en) 2018-01-17
WO2016149088A1 (en) 2016-09-22
SG11201707330RA (en) 2017-10-30

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