CN107632149A - Transparent paper base detection and analysis equipment based on zinc oxide nano wire - Google Patents
Transparent paper base detection and analysis equipment based on zinc oxide nano wire Download PDFInfo
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- CN107632149A CN107632149A CN201710840997.5A CN201710840997A CN107632149A CN 107632149 A CN107632149 A CN 107632149A CN 201710840997 A CN201710840997 A CN 201710840997A CN 107632149 A CN107632149 A CN 107632149A
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- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 title claims abstract description 117
- 238000001514 detection method Methods 0.000 title claims abstract description 33
- 238000004458 analytical method Methods 0.000 title claims abstract description 21
- 239000011787 zinc oxide Substances 0.000 claims abstract description 40
- 239000000758 substrate Substances 0.000 claims abstract description 23
- 238000001035 drying Methods 0.000 claims abstract description 11
- 229920000742 Cotton Polymers 0.000 claims abstract description 5
- 238000001027 hydrothermal synthesis Methods 0.000 claims abstract description 5
- 239000011248 coating agent Substances 0.000 claims abstract description 3
- 238000000576 coating method Methods 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 52
- 239000000243 solution Substances 0.000 claims description 51
- 229920000298 Cellophane Polymers 0.000 claims description 41
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 27
- 108060003951 Immunoglobulin Proteins 0.000 claims description 18
- 102000018358 immunoglobulin Human genes 0.000 claims description 18
- 239000002105 nanoparticle Substances 0.000 claims description 17
- 235000019441 ethanol Nutrition 0.000 claims description 16
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 239000000084 colloidal system Substances 0.000 claims description 11
- 241000283707 Capra Species 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 8
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 5
- 229940098773 bovine serum albumin Drugs 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- DJWUNCQRNNEAKC-UHFFFAOYSA-L zinc acetate Chemical class [Zn+2].CC([O-])=O.CC([O-])=O DJWUNCQRNNEAKC-UHFFFAOYSA-L 0.000 claims description 5
- 235000013904 zinc acetate Nutrition 0.000 claims description 5
- 229920000297 Rayon Polymers 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000004090 dissolution Methods 0.000 claims description 4
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 claims description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 230000002045 lasting effect Effects 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 238000004451 qualitative analysis Methods 0.000 claims description 3
- 238000004445 quantitative analysis Methods 0.000 claims description 3
- 239000012488 sample solution Substances 0.000 claims description 3
- 239000011701 zinc Substances 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- 235000010299 hexamethylene tetramine Nutrition 0.000 claims description 2
- 239000002070 nanowire Substances 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000000523 sample Substances 0.000 claims description 2
- YUYCVXFAYWRXLS-UHFFFAOYSA-N trimethoxysilane Chemical group CO[SiH](OC)OC YUYCVXFAYWRXLS-UHFFFAOYSA-N 0.000 claims description 2
- -1 zinc nitrates hexahydrate Chemical class 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 17
- 238000003018 immunoassay Methods 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract description 4
- 239000004202 carbamide Substances 0.000 abstract description 4
- 239000003513 alkali Substances 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 108010025899 gelatin film Proteins 0.000 abstract 2
- 239000000853 adhesive Substances 0.000 abstract 1
- 230000001070 adhesive effect Effects 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
- 239000002585 base Substances 0.000 description 16
- 239000007788 liquid Substances 0.000 description 6
- 238000009738 saturating Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 229910021389 graphene Inorganic materials 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- XIOUDVJTOYVRTB-UHFFFAOYSA-N 1-(1-adamantyl)-3-aminothiourea Chemical compound C1C(C2)CC3CC2CC1(NC(=S)NN)C3 XIOUDVJTOYVRTB-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011978 dissolution method Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- SSVFMICWXDVRQN-UHFFFAOYSA-N ethanol;sodium Chemical compound [Na].CCO SSVFMICWXDVRQN-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- VKYKSIONXSXAKP-UHFFFAOYSA-N hexamethylenetetramine Chemical compound C1N(C2)CN3CN1CN2C3 VKYKSIONXSXAKP-UHFFFAOYSA-N 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- GRHBQAYDJPGGLF-UHFFFAOYSA-N isothiocyanic acid Chemical compound N=C=S GRHBQAYDJPGGLF-UHFFFAOYSA-N 0.000 description 1
- 230000004298 light response Effects 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
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- 238000005457 optimization Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
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- 235000009566 rice Nutrition 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- LFQCEHFDDXELDD-UHFFFAOYSA-N tetramethyl orthosilicate Chemical compound CO[Si](OC)(OC)OC LFQCEHFDDXELDD-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention relates to transparent paper-based detection and analysis equipment based on zinc oxide nanowires, and belongs to the field of paper-based analysis and detection. The preparation method mainly comprises the following steps: firstly, dissolving a certain amount of cotton linters in a precooled alkali urea solution to form an adhesive solution, forming a gel film after curing a coating film, drying the gel film to prepare transparent paper, and synthesizing zinc oxide nano-wires on the transparent paper by a hydrothermal method to serve as a novel substrate for carrying out sensitive analysis and detection. The invention has the following advantages: (1) the preparation process is simple, easy to operate, easy to post-treat and free of environmental pollution, and belongs to degradable materials; (2) the substrate of the analysis device has flexibility, can be cut and folded; (3) the substrate fully shows the advantages of zinc oxide, and has good biocompatibility and can be used for fluorescence immunoassay. The zinc oxide can improve the fluorescence intensity and the detection sensitivity.
Description
Technical field
The present invention relates to a kind of transparent paper substrate based on zinc oxide nanowire to test and analyze equipment, belongs to paper substrate analysis detection
Field, specifically a kind of flexibility using paper substrate, foldability and zinc oxide can be sheared can improve the excellent of fluorescence intensity
The analyzing detecting method of the portability and detection sensitivity of gesture and then raising analytical equipment.
Background technology
Flexible-paper-base analytical equipment have the function that in technical field of analysis and detection it is more and more important, especially in immune inspection
In terms of survey, paper has good biological compatibility, is a kind of preferably available material.Because traditional analytical equipment is mainly with modeling
The material that material etc. is difficult to degrade is made, and causes serious environmental pollution, and it is imperative to find environmentally friendly material.Paper conduct
A kind of natural material of generally existing attracts attention extensively in recent years, and its abundant raw material is cheap and easy to get, is a kind of
Degradable ideal material, traditional plastic or other material is replaced to significantly reduce environmental pressure with it.The application field of paper is extensive,
Available for clinical diagnosis, the technical field of analysis and detection such as food quality control and environmental monitoring.Cellophane paper is a kind of new paper,
It not only has the property of common paper, and has the advantages of transparent, and by the use of cellophane paper as substrate, it is impermeable to overcome common paper
The shortcomings that bright.
Detection field, some functional material pictures are analyzed in paper substrate：The application of CNT, graphene etc. can improve analysis and set
Standby performance, CNT, which is incorporated into paper, makes paper base material have high conductivity, but the synthesis of CNT and graphene needs
Want special equipment, and be typically complicated and expensive, and and the combination of paper substrate equipment be also troublesome.Therefore, find
Can be strong with the binding ability of paper, and to combine the good functional material of rear performance be vital.
Zinc oxide nanowire is a kind of semi-conducting material, has piezoelectricity, luminescence generated by light, ultraviolet light response and good biology
The properties such as compatibility, it is a kind of multifunctional material having very much using potential quality.Because its bio-compatibility is good, it is strong fluorescence can be improved
Degree, it is possible to and cellophane paper combines, and carries out immune detection, the equipment with reference to after both has the transparency, flexibility of cellophane paper etc.
Advantage has the peculiar property of zinc oxide again, and the presence of zinc oxide causes can be without using amplified signal in fluorescence immunoassay detects
Material, operation are simpler.It is high that this equipment realizes degradable, inexpensive and detection sensitivity, by zinc oxide nanowire with it is transparent
There is presently no relevant report for the analysis detection device that paper combines.
The content of the invention
The present invention is dirty for environment caused by equipment and materials difficult degradation existing for the analytical equipment of current fluorescence immunoassay detection
Amplified signal material etc. is needed to use to complicate detection process in dye problem and immune detection, it is proposed that a kind of by zinc oxide
The method that nano wire is combined with transparent paper base plate, carries out fluorescence immunoassay detection, and the method make it that equipment is simpler, it is soft to have
Property, the degradable green analysis detection device of low cost.
In order to solve the above-mentioned technical problem, technical scheme proposed by the present invention is：It is a kind of based on the saturating of zinc oxide nanowire
Bright paper substrate tests and analyzes equipment, comprises the following steps：
(1) cellophane paper is made using alkalinuria element dissolution in low temperature method, then gives over to follow-up standby；
(2) the colloid seed solution of zinc oxide nano-particle is prepared, it is standby；
(3) 60- in an oven is put in the colloid seed solution that the cellophane paper made in step (1) is placed in step (2)
80 DEG C of a period of times, cellophane paper is taken out into drying from seed solution afterwards, making is loaded with the transparent of zinc oxide nano-particle
Paper, it is standby；
(4) growth-promoting media of zinc oxide nanowire is prepared, it is standby；
(5) cellophane paper for being loaded with zinc oxide nano-particle prepared in step (3) is placed on by step (4) using hydro-thermal method
In growth-promoting media in, put 80-90 DEG C in an oven, a period of time, take out and with deionized water rinsing, room temperature naturally dry, obtain
It is standby to zinc oxide nanowire paper base plate；
(6) fluoroimmunoassay detects, and is printed instead in wax spray on zinc oxide nanowire paper base plate of having grown prepared by step (5)
Region is answered, goat anti-rabbit immunoglobulin is fixed in conversion zone first, it is molten then to add the sample containing rabbit immunoglobulin
Liquid, the goat anti-rabbit immunoglobulin of marked by fluorescein isothiocyanate is added, finally surveys fluorescence intensity；Again in not long zinc oxide
Same operation is carried out on cellophane paper and does contrast test.
Preferably, cellophane paper is made using alkalinuria element dissolution in low temperature method in the step (1), it is specially that cotton linter is molten
Solution forms viscose solution, forms gel mould after curing of coating, be prepared into after drying transparent in the alkali urea liquid of precooling
Paper.
Preferably, the step (2) is comprised the following steps that using the colloid seed solution for preparing zinc oxide nano-particle：
A, the ethanol solution of the water zinc acetates of 1-4mmol/L bis- and the ethanol solution of sodium hydroxide are prepared, respectively
Taking the 20mL solution prepared, equal 60-80 DEG C is stirred vigorously, and respectively obtains corresponding ethanol solution in 2 beakers；
B. take 20mL absolute ethyl alcohol to add in step A in the ethanol solution of two obtained water zinc acetates again, be placed on 60-80
10-30min in DEG C baking oven；
C. the solution obtained in the ethanol solution of the sodium hydroxide obtained in step A and step B is cooled to room temperature, it
The ethanol solution of sodium hydroxide is slowly added in step B to obtained solution afterwards, finally gives mixed solution, by this mixed liquor
60-80 DEG C of lasting 1-5h in baking oven is put into, forms the colloid seed solution of zinc oxide nano-particle.
Preferably, a period of time for putting 60-80 DEG C in an oven described in the step (3) is：1-5min, afterwards will be saturating
Bright paper takes out drying from seed solution, and drying temperature is 60-80 DEG C and keeps 2-5min to dry, and obtains being loaded with zinc oxide nano
The cellophane paper of rice corpuscles.
Preferably, the step (4) prepares the growth-promoting media of zinc oxide nanowire, comprises the following steps that：It is water-soluble to prepare growth
Liquid, it is described growth the aqueous solution be by 20-60mmol/L zinc nitrates hexahydrate, 20-50mmol/L urotropines and
The mixed liquor that 0.2-0.5mol/L ammoniacal liquor is mixed to get.
Preferably, a period of time for 80-90 DEG C in an oven being put in the step (5) is:8-16h, paper is taken out afterwards,
Room temperature naturally dry, zinc oxide nanowire paper base plate is obtained, clap electromicroscopic photograph.
Preferably, the step (6) wax spray on the paper base plate for grown zinc oxide nanowire prints conversion zone, uses front three
TMOS handles conversion zone, fixes 0.1-0.45 μ g/mL goat anti-rabbit immunoglobulin in conversion zone first, fixed
15-30min, the uncombined site of 10-15 μ l 1% bovine serum albumin(BSA) 10-15min closings is added, then adds and contains rabbit
The sample solution of sub- immunoglobulin, reacts 15-30min, and excessive bovine serum albumin(BSA) contains 0.1% polysorbas20 with 200 μ l's
PBS rinse, dry, add the goat anti-rabbit immunoglobulin of 0.1-0.45 μ g/mL marked by fluorescein isothiocyanate, reaction
15-30min, rinse, fluorescence intensity is finally surveyed, according to the height of fluorescence intensity come qualitative and quantitative analysis rabbit immunoglobulin
Concentration；Same operation is carried out on the cellophane paper of not long zinc oxide again and do contrast test, survey its fluorescence intensity, finally also survey
The simply fluorescence intensity of the paper base plate of long zinc oxide.
Beneficial effect
The present invention replaces traditional plastic base using cellophane paper, not only with flexibility but also with the transparency, is easy to
Observation to fluorescence and colorimetric detection.
The cellophane paper that the present invention uses is Biodegradable material, environmentally safe, is easily destroyed.
The present invention cellophane paper is combined with zinc oxide nanowire, not only with transparent, flexible, folding advantage but also with
Zinc oxide can improve the advantages of uniqueness of fluorescence intensity, can be in order to avoid the use of amplified signal material in fluoroscopic examination so that
Operation is simpler.
Analytical equipment of the present invention comprises the following steps：(1) making of cellophane paper；(2) utilize hydro-thermal method long on cellophane paper
Zinc oxide nanowire；(3) fluorescence immunoassay detection is carried out on the transparent paper base plate with zinc oxide nanowire.With following excellent
Point：(1) preparation process is simple, easily operated, is easy to post-process, environmentally safe, belongs to degradation material；(2) this is analyzed
Equipment substrate has flexibility, can shear, foldable；(3) this substrate fully presents the advantages of zinc oxide, has good biology
Compatibility detects available for fluorescence immunoassay.Zinc oxide can improve fluorescence intensity, improve the sensitivity of detection.
Brief description of the drawings
The present invention is described further below in conjunction with the accompanying drawings.
Fig. 1 is pure cellophane paper figure.
Fig. 2 is the transparency figure of pure cellophane paper.
Fig. 3 is the figure for having grown zinc oxide nanowire.
Fig. 4 is the proof figure that zinc oxide can improve fluorescence intensity.
Fig. 5 is the schematic diagram of the transparent paper substrate detection and analysis equipment based on zinc oxide nanowire.
Embodiment
In order to be better understood from the content of patent of the present invention, as described below is the embodiment of the present invention, according to this
The instantiation and accompanying drawing of invention, the present invention can be made by clearer understanding.
A kind of transparent paper substrate detection and analysis equipment based on zinc oxide nanowire, specific implementation step are as follows：
A. the preparation (using the Zhang Lina alkalinuria elements low temperature dissolution method of optimization) of cellophane paper
The preparation of cellophane paper, specific method are as follows：1) by sodium hydroxide, urea and water according to 7:12:81 mass ratio
Wiring solution-forming, it is pre-chilled in -12 DEG C of refrigerator；2) weigh cotton linter 4.5g to be placed in the alkali urea liquid of 100ml precoolings, use
Mechanical agitator, which quickly stirs, causes cotton dissolving, forms viscose solution.Mixing time 15min, mixing speed 3000rpm,
By solution centrifugal bubble removing under 8000rpm；3) prepared viscose solution is poured directly on cleaned glass plate, forms one layer
Uniform film, then it is placed in 5w% sulfuric acid solution and is formed by curing gel, is thoroughly soaked using distilled water；4) by institute's shape
Into gel be placed on PMMA (polymethyl methacrylate) plate, fix four sides using adhesive tape, drying at room temperature, clap electron microscopic picture and see
Fig. 1, and take its transparent print and see Fig. 2.
The surface smoother of cellophane paper as can be seen from Figure 1.
Cellophane paper is transparent relatively good as can be seen from Figure 2, and obtained cellophane paper is placed on pattern, and pattern is still seen
It must be apparent from.
B. the colloid seed solution of zinc oxide nano-particle is prepared
The colloid seed solution of zinc oxide nano-particle is prepared, is comprised the following steps that：1) 4mmol/l two water acetic acid are prepared
The ethanol solution of the ethanol solution of zinc and 4mmol/l sodium hydroxide, the 20ml solution prepared is taken respectively in 2
In individual beaker, equal 70 DEG C are stirred vigorously, and respectively obtain corresponding ethanol solution；2) 20ml absolute ethyl alcohol is taken to add step 1) again
In in the obtained ethanol solution of two water zinc acetates, be placed on 30min in 70 DEG C of baking ovens；3) hydrogen-oxygen that will be obtained in step 1)
Change sodium ethanol solution and step 2) in solution be cooled to room temperature, it is afterwards that the absolute ethyl alcohol of sodium hydroxide is molten
Liquid slowly adds the solution obtained in step 2), finally gives mixed solution；4) mixed liquor obtained in step 3) is put into
60 DEG C of lasting 2h in baking oven, form the colloid seed solution of zinc oxide nano-particle.
C. the cellophane paper for being loaded with zinc oxide nano-particle is made
Cellophane paper is placed in seed solution and is placed in baking oven 3min is kept under the conditions of 60 DEG C, afterwards by cellophane paper from kind
Take out and be placed in 60 DEG C of baking oven in sub- liquid and keep 2min drying, obtain being loaded with the cellophane paper of zinc oxide nano-particle.
D. the growth-promoting media of zinc oxide nanowire is prepared
Growth aqueous solution 125ml is prepared, including：50mmol/l zinc nitrate hexahydrate, the 25mmol/l methylene of ring six
The ammoniacal liquor of urotropine and 0.372mol/l, obtains growth-promoting media.
E. hydro-thermal method long zinc oxide nanowire on cellophane paper is used, forms zinc oxide nanowire paper base plate
The cellophane paper for being loaded with zinc oxide nano-particle is placed in growth-promoting media, and puts 86 DEG C of reaction 16h in an oven, it
After be drawn off and with deionized water rinsing, room temperature naturally dry, obtain zinc oxide nanowire paper base plate, clap electromicroscopic photograph and see figure
3。
F. fluoroimmunoassay detects
Wax spray prints conversion zone on the cellophane paper for grown zinc oxide nanowire, and reaction zone is handled with trimethoxy silane
Domain, 0.45 μ g/ml goat anti-rabbit immunoglobulin, fixed 30min, 10 μ l 1% of addition ox blood are fixed in conversion zone first
The uncombined site of pure protein 15 min closings, then adds the sample solution of rabbit immunoglobulin, reacts 30min, excessive
Bovine serum albumin(BSA) rinsed with PBSs of the 200 μ l containing 0.1% polysorbas20, dry, add 0.45 μ g/ml isothiocyanic acid
Fluorescein-labeled goat anti-rabbit immunoglobulin, 30min is reacted, rinse, finally survey fluorescence intensity, then in the saturating of not long zinc oxide
Same operation is carried out on bright paper and does contrast test, its fluorescence intensity is surveyed, according to the height of fluorescence intensity come qualitative and quantitative analysis
The concentration of rabbit immunoglobulin, has finally also surveyed the fluorescence intensity of the simply paper base plate of long zinc oxide, and experiment measures last right
Than figure result such as Fig. 4.
The paper base plate for simply having grown zinc oxide as can be seen from Figure 4 is no fluorescence intensity, in the saturating of not long zinc oxide
The fluorescence intensity that contrast experiment is on bright paper is about 2400a.u., and on the paper base plate of zinc oxide has been grown, because zinc oxide exists
The fluorescence intensity of fluorescein isothiocynate can be improved under slightly acidic condition, so the fluorescence intensity measured greatly improves, about
For 3900a.u..So measured result is that fluorescence intensity can be greatly improved on the paper base plate for grown zinc oxide, so as to
The sensitivity of detection is improved, therefore carries out immune detection with this analytical equipment and is not required to extra plus amplified signal material, it is convenient fast
It is prompt.
But the present invention is not limited to the concrete technical scheme described in above-described embodiment, all technologies formed using equivalent substitution
Scheme is within the protection domain of application claims.
Claims (7)
- A kind of 1. transparent paper substrate detection and analysis equipment based on zinc oxide nanowire, it is characterised in that:Comprise the following steps：(1) cellophane paper is made using alkalinuria element dissolution in low temperature method, then gives over to follow-up standby；(2) the colloid seed solution of zinc oxide nano-particle is prepared, it is standby；(3) 60-80 DEG C in an oven is put in the colloid seed solution that the cellophane paper made in step (1) is placed in step (2) For a period of time, cellophane paper is taken out into drying from seed solution afterwards, makes the cellophane paper for being loaded with zinc oxide nano-particle, it is standby With；(4) growth-promoting media of zinc oxide nanowire is prepared, it is standby；(5) cellophane paper for being loaded with zinc oxide nano-particle prepared in step (3) is placed in step (4) using hydro-thermal method In growth-promoting media, 80-90 DEG C in an oven is put, a period of time, take out and with deionized water rinsing, room temperature naturally dry, obtain oxygen Change zinc nano wire paper base plate, it is standby；(6) fluoroimmunoassay is detected, and reaction zone is printed in wax spray on zinc oxide nanowire paper base plate of having grown prepared by step (5) Domain, goat anti-rabbit immunoglobulin is fixed in conversion zone first, then adds the sample solution containing rabbit immunoglobulin, then The goat anti-rabbit immunoglobulin of marked by fluorescein isothiocyanate is added, finally surveys fluorescence intensity；Again in the transparent of not long zinc oxide Same operation is carried out on paper and does contrast test.
- 2. the transparent paper substrate detection and analysis equipment according to claim 1 based on zinc oxide nanowire, it is characterised in that：Institute State in step (1) and cellophane paper is made using alkalinuria element dissolution in low temperature method, cotton linter is specially dissolved in the alkalinuria element of precooling In solution, viscose solution is formed, gel mould is formed after curing of coating, is prepared into cellophane paper after drying.
- 3. a kind of transparent paper substrate detection and analysis equipment based on zinc oxide nanowire according to claim 1, its feature exist In：The step (2) is comprised the following steps that using the colloid seed solution for preparing zinc oxide nano-particle：A, the ethanol solution of the water zinc acetates of 1-4mmol/L bis- and the ethanol solution of sodium hydroxide are prepared, is taken respectively In 2 beakers, equal 60-80 DEG C is stirred vigorously the 20mL solution prepared, respectively obtains corresponding ethanol solution；B. take 20mL absolute ethyl alcohol to add in step A in the ethanol solution of two obtained water zinc acetates again, be placed on 60-80 DEG C of baking 10-30min in case；C. the solution obtained in the ethanol solution of the sodium hydroxide obtained in step A and step B is cooled to room temperature, afterwards will The ethanol solution of sodium hydroxide slowly adds in step B obtained solution, finally gives mixed solution, and this mixed liquor is put into 60-80 DEG C of lasting 1-5h in baking oven, form the colloid seed solution of zinc oxide nano-particle.
- 4. the transparent paper substrate detection and analysis equipment according to claim 1 based on zinc oxide nanowire, it is characterised in that：Institute Stating a period of time for putting 60-80 DEG C in an oven described in step (3) is：1-5min, afterwards by cellophane paper from seed solution Drying is taken out, drying temperature is 60-80 DEG C and keeps 2-5min to dry, and obtains being loaded with the cellophane paper of zinc oxide nano-particle.
- 5. the transparent paper substrate detection and analysis equipment according to claim 1 based on zinc oxide nanowire, it is characterised in that：Institute The growth-promoting media that step (4) prepares zinc oxide nanowire is stated, is comprised the following steps that：Prepare the growth aqueous solution, the growth aqueous solution For 20-60mmol/L zinc nitrates hexahydrate, 20-50mmol/L urotropines and 0.2-0.5mol/L ammoniacal liquor are mixed Obtained mixed liquor.
- 6. the transparent paper substrate detection and analysis equipment according to claim 1 based on zinc oxide nanowire, it is characterised in that：Institute State and put 80-90 DEG C in an oven a period of time in step (5) and be:8-16h, paper is taken out afterwards, room temperature naturally dry, obtained Zinc oxide nanowire paper base plate, clap electromicroscopic photograph.
- 7. the transparent paper substrate detection and analysis equipment according to claim 1 based on zinc oxide nanowire, it is characterised in that：Institute State step (6) wax spray on the paper base plate for grown zinc oxide nanowire and print conversion zone, handled and reacted with trimethoxy silane Region, 0.1-0.45 μ g/mL goat anti-rabbit immunoglobulin is fixed in conversion zone first, fixed 15-30min, adds 10-15 The site that μ l 1% bovine serum albumin(BSA) 10-15min closings are not associated with, then adds the sample containing rabbit immunoglobulin Solution, 15-30min is reacted, excessive bovine serum albumin(BSA) is rinsed with the 200 μ l PBS containing 0.1% polysorbas20, dried, then is added Enter the goat anti-rabbit immunoglobulin of 0.1-0.45 μ g/mL marked by fluorescein isothiocyanate, react 15-30min, rinse, finally Fluorescence intensity is surveyed, according to the height of fluorescence intensity come the concentration of qualitative and quantitative analysis rabbit immunoglobulin；Again in not long oxidation Same operation is carried out on the cellophane paper of zinc and does contrast test, surveys its fluorescence intensity, has finally also surveyed the paper of simply long zinc oxide The fluorescence intensity of substrate.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110296946A (en) * | 2019-07-14 | 2019-10-01 | 南京工业大学 | Transparent paper-based ultraviolet-visible light absorption measuring device and paper-based analysis method |
| CN110568192A (en) * | 2019-08-28 | 2019-12-13 | 千藤（南京）环保科技有限公司 | Zinc oxide nanowire fluorescence enhancement-based paper base analysis device and method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546298A (en) * | 2003-12-12 | 2004-11-17 | 武汉大学 | Process for preparing regenerated cellulose films and wires |
| CN101191794A (en) * | 2007-08-27 | 2008-06-04 | 中国科学院理化技术研究所 | Fluorescence chemical biosensor with one-dimensional nanostructure, and preparation method and application thereof |
| CN103693675A (en) * | 2013-12-09 | 2014-04-02 | 济南大学 | Method for preparing paper-based zinc oxide (ZnO) nano-wires |
-
2017
- 2017-09-18 CN CN201710840997.5A patent/CN107632149A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546298A (en) * | 2003-12-12 | 2004-11-17 | 武汉大学 | Process for preparing regenerated cellulose films and wires |
| CN101191794A (en) * | 2007-08-27 | 2008-06-04 | 中国科学院理化技术研究所 | Fluorescence chemical biosensor with one-dimensional nanostructure, and preparation method and application thereof |
| CN103693675A (en) * | 2013-12-09 | 2014-04-02 | 济南大学 | Method for preparing paper-based zinc oxide (ZnO) nano-wires |
Non-Patent Citations (3)
| Title |
|---|
| JONG-IN HAHM: "Zinc Oxide Nanomaterials for Biomedical Fluorescence Detection", 《J NANOSCI NANOTECHNOL.》 * |
| MAZHAR SHER，ET AL.: "Paper-based analytical devices for clinical diagnosis: recent advances in the fabrication techniques and sensing mechanisms", 《EXPERT REV MOL DIAGN. 》 * |
| XIAO LI, ET AL.: "Hydrothermal Growth of ZnO Nanowires on Paper for Flexible", 《INTERNATIONAL CONFERENCE ON NANOTECHNOLOGY》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110296946A (en) * | 2019-07-14 | 2019-10-01 | 南京工业大学 | Transparent paper-based ultraviolet-visible light absorption measuring device and paper-based analysis method |
| CN110568192A (en) * | 2019-08-28 | 2019-12-13 | 千藤（南京）环保科技有限公司 | Zinc oxide nanowire fluorescence enhancement-based paper base analysis device and method |
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