CN105907389B - Noble-metal nanoclusters show the preparation of reagent and show the method for Sweat latent fingerprint - Google Patents
Noble-metal nanoclusters show the preparation of reagent and show the method for Sweat latent fingerprint Download PDFInfo
- Publication number
- CN105907389B CN105907389B CN201610283026.0A CN201610283026A CN105907389B CN 105907389 B CN105907389 B CN 105907389B CN 201610283026 A CN201610283026 A CN 201610283026A CN 105907389 B CN105907389 B CN 105907389B
- Authority
- CN
- China
- Prior art keywords
- solution
- reagent
- show
- pure water
- dissolved
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/58—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing copper, silver or gold
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/117—Identification of persons
- A61B5/1171—Identification of persons based on the shapes or appearances of their bodies or parts thereof
- A61B5/1172—Identification of persons based on the shapes or appearances of their bodies or parts thereof using fingerprinting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/05—Metallic powder characterised by the size or surface area of the particles
- B22F1/054—Nanosized particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/87—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing platina group metals
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Inorganic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Medical Informatics (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Biophysics (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nanotechnology (AREA)
- Manufacture Of Metal Powder And Suspensions Thereof (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention proposes that a kind of noble-metal nanoclusters show the preparation of reagent and show the method for Sweat latent fingerprint.Noble-metal nanoclusters are any one in gold nanoclusters, silver nanoclusters, electrum nano-cluster and platinum noble metals nano-cluster in the present invention；And the noble-metal nanoclusters show reagent and cooperate with preparation method to prepare by ultrasonic-microwave.Show the method for outmoded Sweat latent fingerprint, including prepared by reagent, restrain finger mark, drips aobvious finger mark, washes away loose colour, 6 steps are fixed in finger mark drying and finger mark observation.The present invention covers four kinds of metal nanometre clusters and shows the preparation of reagent and its show the method for outmoded finger mark, and gold, silver, electrum, the platinum fluorescence noble-metal nanoclusters fluorescence quantum yield of preparation are high, and fluorescence duration time is grown, and has hypotoxicity；Finger mark shows operation rapidly and efficiently, can clearly show the outmoded finger mark of 30 days, and effect shows the process for show of reagent better than tradition.
Description
Technical field
The invention belongs to public security criminal technique finger mark to identify field, and in particular to a kind of noble-metal nanoclusters show reagent
The method for preparing and showing Sweat latent fingerprint.
Background technology
Noble-metal nanoclusters refer to be made up of several to dozens of precious metal atoms as a kind of new luminescent material
Particle diameter in 2nm or so cluster, there is relatively stable fluorescence property, high-biocompatibility, hypotoxicity, last decade
Come, the photoluminescent property of noble-metal nanoclusters is mainly used in fluorescence probe detection metal ion, live cell fluorescent imaging, fluorescence biography
Sensor etc..But the research in science and technology concerning criminal matters field rarely has document report with application, is still at the initial stage.
Fingerprint have people it is variant, it is constant throughout one's life, touch thing trace characteristic, the U.S. for having " human body identity card " and " king of evidence "
Claim, to disclosing, confirming that crime and identification criminal have considerable effect.It is distributed with the mastoid process decorative pattern of people's both hands finger finger
Pore, the sweat of pore secretion often contain grease and other small materials.Therefore the Sweat latent fingerprint composition being retained on object
Include the organic matters such as a small amount of water, a small amount of inorganic matter, protein, grease.
Upper century-old history, traditional process for show have 502 smoked aobvious methods, ninhydrin method, DFO methods to finger mark appearing technique
Deng.With the rapid development of nanometer technology, novel nano-material shows the concern for causing researcher applied to latent,
There is the methods of showing finger mark using metal, up-conversion luminescence, magnetic Nano material.
Traditional fluorescent material, such as organic dyestuff, fluorescin, there is cost of manufacture height, high toxicity, fluorescence duration time
It is short and the shortcomings of outmoded finger mark can not be shown.At present, noble-metal nanoclusters synthesis uses traditional heating method and is incubated method, such as
Ying seminars are that protective agent and stabilizer are prepared for the Jenner that fluorescence quantum efficiency is 6% using BSA under the conditions of 12h is incubated
Rice cluster, but the method time loss is long, power consumption is larger, reaction speed is slow, particle diameter is larger, it is low to prepare product property, in real work
In be difficult to be promoted.
The application for showing aspect in latent of gold nanoclusters：Bovine serum albumin(BSA) guarantor is made in patent 201310364617.7
The red fluorescence gold nanoclusters of shield, it is applied to the mark of latent fingerprint on PVDF membrane and adhesive tape substrate and shows
It is existing, but the research is shown only for latent on semi-permeable object, and application is small.Patent 201210443554.X is made
The fluorescence gold nanoclusters of glutathione functionalization, and be applied to silicon chip, Sweat latent fingerprint, blood finger mark show on glass, widen
The application that finger mark shows.Above-mentioned two research incubates heating means in preparation process using tradition, takes longer (12h-
72h), it is cumbersome, do not possess the advantages of green high-efficient, combined coefficient is relatively low, and does not explore and show outmoded effect.
From the foregoing, it will be observed that in existing finger mark appearing technique, there is cost of manufacture height, high toxicity, fluorescence for conventional fluorescent material
Duration is short and the shortcomings of can not showing outmoded finger mark；Gold nanoclusters there is time-consuming longer (12h-72h), cumbersome,
The problems such as combined coefficient is relatively low.
The content of the invention
To solve above mentioned problem present in existing finger mark appearing technique, the present invention proposes that a kind of noble-metal nanoclusters show
The preparation of reagent and the method for showing Sweat latent fingerprint.
A kind of noble-metal nanoclusters of the present invention show the preparation method of reagent, it is characterised in that the noble-metal nanoclusters
For any one in gold nanoclusters, silver nanoclusters, electrum nano-cluster and platinum noble metals nano-cluster；And the noble metal is received
Rice cluster shows reagent and prepared by ultrasound-microwave cooperating preparation method.
Further, the noble-metal nanoclusters show reagent and prepared by ultrasound-microwave cooperating preparation method, including,
The method that gold nanoclusters show reagent is prepared, is comprised the following steps：
(1) solution is prepared, and gold chloride is dissolved in into the solution that pure water is configured to 0.01 mol/L；Bovine serum albumin(BSA) is at 37 DEG C
Under the conditions of be dissolved in pure water and be configured to 50 mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；
(2) hybrid reaction, it is by volume 1:1, chlorauric acid solution is added into bovine serum albumin solution, will be mixed molten
Liquid reacts 3min in the presence of magnetic stirring apparatus；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise into the chlorauric acid solution and bovine serum albumin obtained by step (2)
In the mixed liquor of white solution, regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40 ~ 60s of microwave radiation, made
Obtain gold nanoclusters and show reagent；
The method that silver nanoclusters show reagent is prepared, is comprised the following steps：
(1) solution is prepared, and silver nitrate is dissolved in into the solution that pure water is configured to 0.01 mol/L；Bovine serum albumin(BSA) is at 37 DEG C
Under the conditions of be dissolved in pure water and be configured to 50 mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；
(2) hybrid reaction, it is by volume 1:1, silver nitrate solution is added into bovine serum albumin solution, will be mixed molten
Liquid reacts 3min in the presence of magnetic stirring apparatus；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise into silver nitrate solution obtained by step (2) and ox blood is pure
In the mixed liquor of protein solution, regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40 ~ 60s of microwave radiation, made
Obtain silver nanoclusters and show reagent；
The method that gold and silver nano-cluster shows reagent is prepared, is comprised the following steps：
(1) solution is prepared, and gold chloride is dissolved in into the solution that pure water is configured to 0.01 mol/L；Silver nitrate is dissolved in pure water and matched somebody with somebody
It is set to 0.01 mol/L solution；Bovine serum albumin(BSA) is dissolved in pure water under the conditions of 37 DEG C and is configured to 50 mg/mL solution；By hydrogen
Potassium oxide is dissolved in pure water and is configured to 1 mol/L solution；
(2) hybrid reaction, it is by volume 1:1:2, chlorauric acid solution, silver nitrate solution are added into bovine serum albumin(BSA)
Solution, mixed solution is reacted into 3min in the presence of magnetic stirring apparatus；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise in the mixed liquor obtained by step (2), and regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40 ~ 60s of microwave radiation, made
Obtain silver nanoclusters and show reagent；
The method that platinum cluster shows reagent is prepared, is comprised the following steps：
(1) solution is prepared, and chloroplatinic acid is dissolved in into the solution that pure water is configured to 0.01 mol/L；Bovine serum albumin(BSA) is 37
Pure water is dissolved under the conditions of DEG C and is configured to 50 mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；
(2) hybrid reaction, it is by volume 1:1, platinum acid chloride solution is added into bovine serum albumin solution, will be mixed molten
Liquid reacts 3min in the presence of magnetic stirring apparatus；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise into platinum acid chloride solution obtained by step (2) and ox blood is pure
In the mixed liquor of protein solution, regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40 ~ 60s of microwave radiation, made
Obtain silver nanoclusters and show reagent.
Present invention additionally comprises showing the method that reagent shows outmoded Sweat latent fingerprint using above-mentioned noble-metal nanoclusters, including with
Lower step：
Prepared by S1, reagent, prepare noble-metal nanoclusters and show reagent；
S2, finger mark is restrained, after volunteer will hand-wash net dry, the stamp sweat on glass, plastics, preservative film, metal object
Latent, obtain finger mark sample.
The aobvious finger mark of S3, drop, Sweat latent fingerprint made from the aobvious step S2 of reagent drop, reaction 1- will be shown made from step S1
3h；
S4, loose colour is washed away, the Sweat latent fingerprint loose colour after step S3 drops show is washed away using ultra-pure water；
S5, finger mark drying, step S4 are washed away the drying of the Sweat latent fingerprint after loose colour；
S6, finger mark observation are fixed, in the case where showing observing system, the Sweat latent fingerprint image after the S5 drying of observation fixing step；
Characterized in that, the noble-metal nanoclusters are gold nanoclusters, silver nanoclusters, electrum nano-cluster and the expensive gold of platinum
Belong to any one in nano-cluster.
Further, in the step S1 prepared by reagent, including,
The method that gold nanoclusters show reagent is prepared, is comprised the following steps：
(1) solution is prepared, and gold chloride is dissolved in into the solution that pure water is configured to 0.01 mol/L；Bovine serum albumin(BSA) is 37
Pure water is dissolved under the conditions of DEG C and is configured to 50 mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；
(2) hybrid reaction, it is by volume 1:1, chlorauric acid solution is added into bovine serum albumin solution, will be mixed molten
Liquid reacts 3min in the presence of magnetic stirring apparatus；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise into chlorauric acid solution obtained by step (2) and ox blood is pure
In the mixed liquor of protein solution, regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40 ~ 60s of microwave radiation, made
Obtain gold nanoclusters and show reagent；
The method that silver nanoclusters show reagent is prepared, is comprised the following steps：
(1) solution is prepared, and silver nitrate is dissolved in into the solution that pure water is configured to 0.01 mol/L；Bovine serum albumin(BSA) is 37
Pure water is dissolved under the conditions of DEG C and is configured to 50 mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；
(2) hybrid reaction, it is by volume 1:1, silver nitrate solution is added into bovine serum albumin solution, will be mixed molten
Liquid reacts 3min in the presence of magnetic stirring apparatus；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise into silver nitrate solution obtained by step (2) and ox blood is pure
In the mixed liquor of protein solution, regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40 ~ 60s of microwave radiation, made
Obtain silver nanoclusters and show reagent；
The method that gold and silver nano-cluster shows reagent is prepared, is comprised the following steps：
(1) solution is prepared, and gold chloride is dissolved in into the solution that pure water is configured to 0.01 mol/L；Silver nitrate is dissolved in pure water and matched somebody with somebody
It is set to 0.01 mol/L solution；Bovine serum albumin(BSA) is dissolved in pure water under the conditions of 37 DEG C and is configured to 50 mg/mL solution；By hydrogen
Potassium oxide is dissolved in pure water and is configured to 1 mol/L solution；
(2) hybrid reaction, it is by volume 1:1:2, chlorauric acid solution, silver nitrate solution are added into bovine serum albumin(BSA)
Solution, mixed solution is reacted into 3min in the presence of magnetic stirring apparatus；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise in the mixed liquor obtained by step (2), and regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40 ~ 60s of microwave radiation, made
Obtain silver nanoclusters and show reagent；
The method that platinum cluster shows reagent is prepared, is comprised the following steps：
(1) solution is prepared, and chloroplatinic acid is dissolved in into the solution that pure water is configured to 0.01 mol/L；Bovine serum albumin(BSA) is 37
Pure water is dissolved under the conditions of DEG C and is configured to 50 mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；
(2) hybrid reaction, it is by volume 1:1, platinum acid chloride solution is added into bovine serum albumin solution, will be mixed molten
Liquid reacts 3min in the presence of magnetic stirring apparatus；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise into platinum acid chloride solution obtained by step (2) and ox blood is pure
In the mixed liquor of protein solution, regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40 ~ 60s of microwave radiation, made
Obtain silver nanoclusters and show reagent.
Further, the step S2 restrains finger mark, including, the resting period of the finger mark sample of acquisition is 7-30 days.
Further, the step S3 drops show finger mark, including, received with the noble metal obtained by rubber head dropper aspiration step S1
Rice cluster shows reagent, and making it, drop is aobvious clockwise along finger mark mastoid process streakline, and fully reacts 2h with finger mark material.
Further, step S5 finger marks drying, including, in baking oven machine vacuum, dry under the conditions of 37 DEG C.
Further, the step S6 finger marks observation, including, show observing system to utilize alternative light source, coloured filter
What light microscopic, digital single-lens reflex camera were built shows observing system.
Fermi wavelength of the present invention using noble-metal nanoclusters size closer to metal electron, itself and protein, amino acid
In sulfydryl combine, there is the characteristics of good fluorescent characteristic, and by ultrasound and microwave cooperating effect, prepare gold, silver, gold and silver
Alloy, platinum fluorescence metal nano-cluster show reagent, can fast and efficiently show outmoded finger mark.
The present invention has following advantageous effects：
1. the present invention covers four kinds of metal nanometre clusters and shows the preparation of reagent and its show the method for outmoded finger mark, this shows
Tube- nursery is compared to quick (about 1h or so) prepared by the method for other patent reports；
2. the gold, silver prepared, electrum, platinum fluorescence noble-metal nanoclusters fluorescence quantum yield are high, and during phosphor persistence
Between it is long, there is hypotoxicity；
3. finger mark shows operation rapidly and efficiently, it can clearly show the outmoded finger mark of 30 days；
4. the effect that four kinds of noble-metal nanoclusters prepared by the present invention show outmoded finger mark, which is superior to tradition, shows reagent
Show effect；
5. finger mark, which shows observing system, can preferably fix finger mark information, be advantageous to test sensitivity.
Brief description of the drawings
Fig. 1 is gold nanoclusters, silver nanoclusters, electrum nano-cluster and platinum cluster composition principle figure.
Fig. 2 is hydro-thermal method, ultrasonic method, microwave method and ultrasound-microwave cooperating method to gold nanoclusters fluorescence intensity and average grain
The influence schematic diagram in footpath.
Fig. 3 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster fluorescence color figure.
Fig. 4 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster ultraviolet spectrogram.
Fig. 5 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster transmission electron microscope picture.
Fig. 6 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, the infrared spectrogram of platinum cluster.
Fig. 7 is that finger mark shows observing system and shows principle schematic.
Fig. 8 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster show outmoded finger mark in glass object
Figure.
Fig. 9 is that the present invention shows preparation method of reagent thereof flow chart.
Figure 10 is that the present invention shows outmoded Sweat latent fingerprint method flow diagram.
Embodiment
Accompanying drawing 1 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster composition principle figure, bovine serum albumin
It is complicated containing the amino acid such as tyrosine, cysteine, molecular structure in vain.Stable structure can be formed with gold, silver, pt atom.Adopt
It is nano-cluster Stability Analysis of Structures prepared by stabilizer reducing agent with bovine serum albumin(BSA).
Fig. 2 is hydro-thermal method, ultrasonic method, microwave method and ultrasound-microwave cooperating method to gold nanoclusters fluorescence intensity and average grain
The influence schematic diagram in footpath.Obviously, the nanocluster fluorescence intensity that prepared by ultrasound-microwave cooperating heating is substantially better than with particle size
Other preparation methods；Nanocluster size prepared by microwave method is smaller, and this makes solution thermally equivalent relevant with microwave heating；Ultrasonic method
The particle diameter of preparation is more bigger than microwave method, but fluorescence property is excellent；Nano-cluster performance prepared by traditional heating method is worst.It is super
By ultrasonic method, each advantage is combined sound-microwave cooperating heating with microwave method, the characteristics of having fully demonstrated efficient green.Its is efficient
Property be embodied in and can not only reduce particle diameter, dispersion effect and fluorescence property enhancing, moreover it is possible to substantially reduce the reaction time, instead
Efficiency is answered to improve；Its green is embodied in BSA bioactivity in building-up process and not changed, whole course of reaction safety,
Reaction time, which reduces, causes power consumption to reduce, while reduces the amount of reagent of preparation same amount nano-cluster needs.
Fig. 3 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster fluorescence color figure（Abcd is followed successively by gold
Nano-cluster, silver nanoclusters, electrum nano-cluster, platinum cluster）.It can be seen that gold nanoclusters irradiate in alternative light source
Under send fluorescent red-orange；Silver nanoclusters send fluorescent orange under alternative light source irradiation；Electrum nano-cluster is in more ripples
Yellow fluorescence is sent under Duan Guangyuan irradiations；Platinum cluster sends blue-green fluorescent under alternative light source irradiation.Noble metal nano
The fluorescence intensity of cluster is strong.
Fig. 4 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster ultraviolet spectrogram（Abcd is followed successively by gold
Nano-cluster, silver nanoclusters, electrum nano-cluster, platinum cluster）.It is expensive as we know from the figure under 280 nm or so ultraviolet radioactive
Metal nanometre cluster has a stronger ultraviolet absorption peak, and tryptophan and tyrosine residue UV absorption in bovine serum albumin(BSA)
Peak also is located at 280 nm or so.Bovine serum albumin(BSA), which is wrapped in outside precious metal atom, plays protection stabilization, therefore nano-cluster
Ultraviolet spectra property it is close with bovine serum albumin(BSA), this explanation ultrasound-microwave heating coordination technique prepares nano-cluster and not destroyed
The bioactivity of bovine serum albumin(BSA).
Fig. 5 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster transmission electron microscope picture.（Abcd is followed successively by
Gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster）.Find that nano-cluster dispersing uniformity is good as we know from the figure,
Its average particle size it is small about 3-5nm. this compared with particle diameter that Du Juan in 2013 is reported is 13.3 nm Conventional nano particle, chi
It is very little to be much smaller than nano-particle.Therefore nano-cluster is bigger compared to nano-particle energy level discontinuity, and the ability of transition occurs for electronics
Stronger, caused fluorescence is stronger.
Fig. 6 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, the infrared spectrogram of platinum cluster（Abcd is followed successively by
Gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster）.It can be seen that the characteristic absorption peak of these three solution
Significant change does not occur, but sulfydryl characteristic absorption peak of the nano-cluster at 2300 cm-1 or so place disappears, and illustrates to pass through Ultrasonic Heating
Reaction bovine serum albumin(BSA) molecule has successfully been modified to the surface of nano-cluster.And the characteristic absorption peak of bovine serum albumin(BSA) itself
Do not changed significantly after before the reaction, illustrate that the conformation of protein changes without obvious.
Fig. 7 is that finger mark shows observing system and shows principle schematic.Utilize alternative light source, colour light filter mirror, number
Slr camera, which is independently built, to be shown observing system and carries out observation fixation to Sweat latent fingerprint., will as we know from the figure under dark condition
The Sweat latent fingerprint that alternative light source irradiation stamp is shown by gold nanoclusters powder, fluorescent red-orange caused by finger mark pass through coloured filter
Light microscopic is recorded by digital single-lens reflex camera, and finger mark mastoid process streakline and its minutia are fixed up.Its of finger mark feature is not reflected
He can not pass through colour light filter mirror so that can not be by cameras record by veiling glare.Finger mark shows that effect is good, can examine the one-level for seeing finger mark, two
Level even three-level feature.
Fig. 8 is gold nanoclusters, silver nanoclusters, electrum nano-cluster, platinum cluster show outmoded finger mark in glass object
Figure.From in figure, the effect that noble-metal nanoclusters show outmoded finger mark is good, show reagent better than traditional powder.Wherein platinum nanometer
Cluster shows best results, and finger mark mastoid process streakline is smooth, and the minutia such as spillikin, dot protrudes.Electrum nano-cluster, Yin Na
The effect that shows of rice cluster is taken second place, and it is slightly worse that gold nanoclusters show effect.
Embodiment 1
A kind of noble-metal nanoclusters of the present invention show the preparation method of reagent, comprise the following steps：1% gold chloride is molten
0.01 mol/L solution is configured in pure water；Bovine serum albumin(BSA) is dissolved in pure water under the conditions of 37 DEG C and is configured to 50 mg/mL
Solution；Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；By the good chlorauric acid solution of above-mentioned configuration, bovine serum albumin
White solution by volume 1:1 mixing, is placed under magnetic stirring apparatus and reacts 3min；Slowly potassium hydroxide solution is added dropwise with transfusion needle
Into the mixed liquor of chlorauric acid solution and bovine serum albumin solution described in this step, regulation pH is 12, now used 0.01
Mol/L chlorauric acid solution, 50 mg/mL bovine serum albumin solution, 1 mol/L potassium hydroxide solution volume ratio are 10:
10:1；The mixed solution that pH is 12 is first placed in ultrasonic 1h in ultrasonic washing instrument；It is placed in again in household microwave oven, setting is high
(800W) microwave radiation 50s under the conditions of fire, obtained gold nanoclusters show reagent.
Embodiment 2
A kind of noble-metal nanoclusters of the present invention show the preparation method of reagent, comprise the following steps：1% silver nitrate is molten
0.01 mol/L solution is configured in pure water；Bovine serum albumin(BSA) is dissolved in pure water under the conditions of 37 DEG C and is configured to solution；Will
Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；The good silver nitrate solution of above-mentioned configuration, bovine serum albumin solution are pressed
Volume ratio 1:1 mixing, is placed under magnetic stirring apparatus and reacts 3min；Potassium hydroxide solution is slowly added drop-wise to this step with transfusion needle
In the mixed liquor of the silver nitrate solution and bovine serum albumin solution, regulation pH is 12,0.01 mol/L's now used
Silver nitrate solution, 50 mg/mL bovine serum albumin solution, 1 mol/L potassium hydroxide solution volume ratio are 10:10:1；Will
The mixed solution that pH is 12 is first placed in ultrasonic 1h in ultrasonic washing instrument；It is placed in again in household microwave oven, under the conditions of the high fire of setting
(800W) microwave radiation 60s, obtained silver nanoclusters show reagent.
Embodiment 3
A kind of noble-metal nanoclusters of the present invention show the preparation method of reagent, comprise the following steps：1% gold chloride is molten
0.01 mol/L solution is configured in pure water；1% silver nitrate is dissolved in the solution that pure water is configured to 0.01 mol/L；By ox
Seralbumin is dissolved in pure water under the conditions of 37 DEG C and is configured to solution；Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；
By the good chlorauric acid solution of above-mentioned configuration, silver nitrate solution, bovine serum albumin solution by volume 1:1:2 mixing, are placed in magnetic
3min is reacted under power agitator；Slowly potassium hydroxide solution is added drop-wise in above-mentioned mixed liquor with transfusion needle, regulation pH is 12, this
Shi Suoyong 0.01 mol/L chlorauric acid solution, 0.01 mol/L silver nitrate solution, 50 mg/mL bovine serum albumin(BSA)
Solution, 1 mol/L potassium hydroxide solution volume ratio are 5:5:10:1；The mixed solution that pH is 12 is first placed in ultrasonic washing
Ultrasonic 1h in instrument；It is placed in again in household microwave oven, sets (800W) microwave radiation 40s under the conditions of high fire, gold and silver nano-cluster is made
Show reagent.
Embodiment 4
A kind of noble-metal nanoclusters of the present invention show the preparation method of reagent, comprise the following steps：Chloroplatinic acid is dissolved in pure
Water is configured to 0.01 mol/L solution；Bovine serum albumin(BSA) is dissolved in pure water under the conditions of 37 DEG C and is configured to solution；By hydrogen-oxygen
Change potassium is dissolved in pure water and is configured to 1 mol/L solution；The good platinum acid chloride solution of above-mentioned configuration, bovine serum albumin solution are pressed into volume
Than 1:1 mixing, is placed under magnetic stirring apparatus and reacts 3min；Slowly potassium hydroxide solution is added drop-wise to described in this step with transfusion needle
In the mixed liquor of platinum acid chloride solution and bovine serum albumin solution, regulation pH is 12,0.01 mol/L now used chlorine platinum
Acid solution, 50 mg/mL bovine serum albumin solution, 1 mol/L potassium hydroxide solution volume ratio are 10:10:1；It is by pH
12 mixed solution is first placed in ultrasonic 1h in ultrasonic washing instrument；It is placed in again in household microwave oven, under the conditions of the high fire of setting
(800W) microwave radiation 45s, obtained platinum cluster show reagent.
Embodiment 5
A kind of method for being shown outmoded Sweat latent fingerprint using noble-metal nanoclusters, is comprised the following steps：
S1,1% gold chloride is dissolved in the solution that pure water is configured to 0.01 mol/L；By bovine serum albumin(BSA) in 37 DEG C of bars
Pure water is dissolved under part and is configured to 50 mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；By above-mentioned configuration
Good chlorauric acid solution, bovine serum albumin solution by volume 1:1 mixing, is placed under magnetic stirring apparatus and reacts 3min；With defeated
Potassium hydroxide solution is slowly added drop-wise in the mixed liquor of chlorauric acid solution and bovine serum albumin solution described in this step by liquid pin,
It is 12 to adjust pH, 0.01 mol/L now used chlorauric acid solution, 50 mg/mL bovine serum albumin solution, 1 mol/
L potassium hydroxide solution volume ratio is 10:10:1；The mixed solution that pH is 12 is first placed in ultrasonic 1h in ultrasonic washing instrument；
It is placed in again in household microwave oven, sets (800W) microwave radiation 50s under the conditions of high fire, obtained gold nanoclusters shows reagent；
After S2, volunteer will hand-wash net dry, the stamp Sweat latent fingerprint on glass, finger mark sample, finger mark sample pendulum are obtained
Put that spacing is moderate, to imitate the outmoded Sweat latent fingerprint left on criminal-scene, this experiment finger mark sample is deposited under field conditions (factors)
In clean pallet, resting period 30d；
S3, with the gold nanoclusters obtained by rubber head dropper aspiration step S1 show reagent, make it suitable along finger mark mastoid process streakline
Hour hands drop is aobvious, and fully reacts 1h with finger mark material；
S4, the Sweat latent fingerprint loose colour after step S3 drops show is washed away with ultra-pure water；
S5, step S4 washed away the Sweat latent fingerprint after loose colour in baking oven machine vacuum, dry 0.5h under the conditions of 37 DEG C；
S6, independently built using alternative light source, colour light filter mirror, digital single-lens reflex camera and show observing system to fluorescence sweat
Latent carries out observation fixation, and under dark condition, alternative light source is irradiated to the Sweat latent fingerprint shown by gold nanoclusters, finger mark
Caused fluorescence is recorded through colour light filter mirror by digital single-lens reflex camera, under finger mark mastoid process streakline and its minutia are fixed
Come.
Embodiment 6
A kind of method for being shown outmoded Sweat latent fingerprint using noble-metal nanoclusters, is comprised the following steps：
S1,1% silver nitrate is dissolved in the solution that pure water is configured to 0.01 mol/L；By bovine serum albumin(BSA) in 37 DEG C of bars
Pure water, which is dissolved in, under part is configured to solution；Potassium hydroxide solution pure water is configured to 1 mol/L solution；By the good nitric acid of above-mentioned configuration
Silver-colored solution, bovine serum albumin solution by volume 1:1 mixing, is placed under magnetic stirring apparatus and reacts 3min；It is slow with transfusion needle
Potassium hydroxide is dissolved in and is added drop-wise in the mixed liquor of silver nitrate solution and bovine serum albumin solution described in this step, regulation pH is
12,0.01 mol/L now used silver nitrate solution, 50 mg/mL bovine serum albumin solution, 1 mol/L hydrogen-oxygen
It is 10 to change potassium solution volume ratio:10:1；The mixed solution that pH is 12 is first placed in ultrasonic 1h in ultrasonic washing instrument；It is placed in again
In household microwave oven, (800W) microwave radiation 60s under the conditions of high fire is set, obtained silver nanoclusters show reagent；
After S2, volunteer will hand-wash net dry, the stamp Sweat latent fingerprint on plastics, finger mark sample, finger mark sample pendulum are obtained
Put that spacing is moderate, to imitate the outmoded Sweat latent fingerprint left on criminal-scene, this experiment finger mark sample is deposited under field conditions (factors)
In clean pallet, resting period 30d；
S3, with the silver nanoclusters obtained by rubber head dropper aspiration step S1 show reagent, make it suitable along finger mark mastoid process streakline
Hour hands drop is aobvious, and fully reacts 2h with finger mark material；
S4, the Sweat latent fingerprint loose colour after step S3 drops show is washed away with ultra-pure water；
S5, step S4 washed away the Sweat latent fingerprint after loose colour in baking oven machine vacuum, dry 10min under the conditions of 37 DEG C；
S6, independently built using alternative light source, colour light filter mirror, digital single-lens reflex camera and show observing system to fluorescence sweat
Latent carries out observation fixation, and under dark condition, alternative light source is irradiated to the Sweat latent fingerprint shown by silver nanoclusters, finger mark
Caused fluorescence is recorded through colour light filter mirror by digital single-lens reflex camera, under finger mark mastoid process streakline and its minutia are fixed
Come.
Embodiment 7
A kind of method for being shown outmoded Sweat latent fingerprint using noble-metal nanoclusters, is comprised the following steps：
S1,1% gold chloride is dissolved in the solution that pure water is configured to 0.01 mol/L；1% silver nitrate is dissolved in into pure water to match somebody with somebody
It is set to 0.01 mol/L solution；Bovine serum albumin(BSA) is dissolved in pure water under the conditions of 37 DEG C and is configured to solution；By potassium hydroxide
It is dissolved in pure water and is configured to 1 mol/L solution；The good chlorauric acid solution of above-mentioned configuration, silver nitrate solution, bovine serum albumin(BSA) is molten
Liquid by volume 1:1:2 mixing, are placed under magnetic stirring apparatus and react 3min；Slowly potassium hydroxide solution is added drop-wise to transfusion needle
In above-mentioned mixed liquor, regulation pH is 12, and 0.01 mol/L now used chlorauric acid solution, 0.01 mol/L silver nitrate is molten
Liquid, 50 mg/mL bovine serum albumin solution, 1 mol/L potassium hydroxide solution volume ratio are 5:5:10:1；It is 12 by pH
Mixed solution be first placed in ultrasonic 1h in ultrasonic washing instrument；It is placed in household microwave oven, is set under the conditions of high fire (800W) again
Microwave radiation 40s, gold and silver nano-cluster is made and shows reagent；
After S2, volunteer will hand-wash net dry, the stamp Sweat latent fingerprint on preservative film, finger mark sample, finger mark sample are obtained
Put that spacing is moderate, to imitate the outmoded Sweat latent fingerprint left on criminal-scene, this experiment finger mark sample is deposited under field conditions (factors)
It is placed in clean pallet, resting period 30d；
S3, with the gold and silver nano-cluster obtained by rubber head dropper aspiration step S1 show reagent, make it along finger mark mastoid process streakline
Drop is aobvious clockwise, and fully reacts 3h with finger mark material；
S4, the Sweat latent fingerprint loose colour after step S3 drops show is washed away with ultra-pure water；
S5, step S4 washed away the Sweat latent fingerprint after loose colour in baking oven machine vacuum, dry 10min under the conditions of 37 DEG C；
S6, independently built using alternative light source, colour light filter mirror, digital single-lens reflex camera and show observing system to fluorescence sweat
Latent carries out observation fixation, under dark condition, alternative light source is irradiated to the Sweat latent fingerprint shown by gold and silver nano-cluster, referred to
Fluorescence caused by print is recorded through colour light filter mirror by digital single-lens reflex camera, under finger mark mastoid process streakline and its minutia are fixed
Come.
A kind of method for being shown outmoded Sweat latent fingerprint using noble-metal nanoclusters of embodiment 8, is comprised the following steps：
S1, chloroplatinic acid is dissolved in the solution that pure water is configured to 0.01 mol/L；By bovine serum albumin(BSA) under the conditions of 37 DEG C
It is dissolved in pure water and is configured to solution；Potassium hydroxide is dissolved in pure water and is configured to 1 mol/L solution；The good chloroplatinic acid of above-mentioned configuration is molten
Liquid, bovine serum albumin solution by volume 1:1 mixing, is placed under magnetic stirring apparatus and reacts 3min；With transfusion needle slowly by hydrogen
Potassium oxide solution is added drop-wise in the mixed liquor of platinum acid chloride solution and bovine serum albumin solution described in this step, and regulation pH is 12,
0.01 mol/L now used platinum acid chloride solution, 50 mg/mL bovine serum albumin solution, 1 mol/L potassium hydroxide
Liquor capacity ratio is 10:10:1；The mixed solution that pH is 12 is first placed in ultrasonic 1h in ultrasonic washing instrument；Family expenses are placed in again
In micro-wave oven, (800W) microwave radiation 45s under the conditions of high fire is set, obtained platinum cluster shows reagent；
After S2, volunteer will hand-wash net dry, the stamp Sweat latent fingerprint on metal, finger mark sample, finger mark sample pendulum are obtained
Put that spacing is moderate, to imitate the outmoded Sweat latent fingerprint left on criminal-scene, this experiment finger mark sample is deposited under field conditions (factors)
In clean pallet, resting period 30d；
S3, with the platinum cluster obtained by rubber head dropper aspiration step S1 show reagent, make it suitable along finger mark mastoid process streakline
Hour hands drop is aobvious, and fully reacts 2.5h with finger mark material；
S4, the Sweat latent fingerprint loose colour after step S3 drops show is washed away with ultra-pure water；
S5, step S4 washed away the Sweat latent fingerprint after loose colour in baking oven machine vacuum, dry 20min under the conditions of 37 DEG C；
S6, independently built using alternative light source, colour light filter mirror, digital single-lens reflex camera and show observing system to fluorescence sweat
Latent carries out observation fixation, and under dark condition, alternative light source is irradiated to the Sweat latent fingerprint shown by platinum cluster, finger mark
Caused fluorescence is recorded through colour light filter mirror by digital single-lens reflex camera, under finger mark mastoid process streakline and its minutia are fixed
Come.
The present invention is acted on by ultrasound and microwave cooperating, is prepared gold, silver, electrum, platinum fluorescence metal nano-cluster and is shown
Reagent, it can fast and efficiently show outmoded finger mark.This method is simple for process；Gold, silver, electrum, the platinum of preparation are glimmering
Light noble-metal nanoclusters fluorescence quantum yield is high, and fluorescence duration time is grown, and has hypotoxicity；30 days outmoded can clearly be shown
Finger mark；Autonomous build shows observing system and can preferably fix finger mark information, is advantageous to test sensitivity.
The above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to preferred embodiment to this hair
It is bright to be described in detail, it will be understood by those within the art that, technical scheme can be modified
Or equivalent substitution, without departing from the objective and scope of technical solution of the present invention, it all should cover the claim in the present invention
Among scope.
Claims (7)
1. a kind of noble-metal nanoclusters show the preparation method of reagent, it is characterised in that the noble-metal nanoclusters are silver nanoparticle
Any one in cluster, electrum nano-cluster and platinum noble metals nano-cluster；And the noble-metal nanoclusters show reagent and passed through
It is prepared by ultrasound-microwave cooperating preparation method；The noble-metal nanoclusters show the preparation method of reagent, including,
The method that silver nanoclusters show reagent is prepared, is comprised the following steps：
(1) solution is prepared, and silver nitrate is dissolved in into the solution that pure water is configured to 0.01mol/L；Bovine serum albumin(BSA) is in 37 DEG C of conditions
Under be dissolved in pure water and be configured to 50mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1mol/L solution；
(2) hybrid reaction, it is by volume 1:1, silver nitrate solution is added into bovine serum albumin solution, by mixed solution in magnetic
3min is reacted in the presence of power agitator；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise into silver nitrate solution obtained by step (2) and bovine serum albumin(BSA) is molten
In the mixed liquor of liquid, regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40~60s of microwave radiation, silver is made
Nano-cluster shows reagent；
The method that gold and silver nano-cluster shows reagent is prepared, is comprised the following steps：
(1) solution is prepared, and gold chloride is dissolved in into the solution that pure water is configured to 0.01mol/L；Silver nitrate is dissolved in pure water and is configured to
0.01mol/L solution；Bovine serum albumin(BSA) is dissolved in pure water under the conditions of 37 DEG C and is configured to 50mg/mL solution；By potassium hydroxide
It is dissolved in pure water and is configured to 1mol/L solution；
(2) hybrid reaction, it is by volume 1:1:2, chlorauric acid solution, silver nitrate solution are added into bovine serum albumin solution,
Mixed solution is reacted into 3min in the presence of magnetic stirring apparatus；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise in the mixed liquor obtained by step (2), and regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40~60s of microwave radiation, silver is made
Nano-cluster shows reagent；
The method that platinum cluster shows reagent is prepared, is comprised the following steps：
(1) solution is prepared, and chloroplatinic acid is dissolved in into the solution that pure water is configured to 0.01mol/L；Bovine serum albumin(BSA) is in 37 DEG C of conditions
Under be dissolved in pure water and be configured to 50mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1mol/L solution；
(2) hybrid reaction, it is by volume 1:1, platinum acid chloride solution is added into bovine serum albumin solution, by mixed solution in magnetic
3min is reacted in the presence of power agitator；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise into platinum acid chloride solution obtained by step (2) and bovine serum albumin(BSA) is molten
In the mixed liquor of liquid, regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40~60s of microwave radiation, silver is made
Nano-cluster shows reagent.
2. a kind of noble-metal nanoclusters show the method that reagent shows outmoded Sweat latent fingerprint, comprise the following steps：Will using right
The noble-metal nanoclusters for asking 1 preparation method to prepare show reagent and show outmoded Sweat latent fingerprint, comprise the following steps,
Prepared by S1, reagent, prepare noble-metal nanoclusters and show reagent；
S2, finger mark is restrained, after volunteer will hand-wash net dry, stamp sweat is latent on glass, plastics, preservative film, metal object refers to
Print, obtain finger mark sample；
The aobvious finger mark of S3, drop, Sweat latent fingerprint made from the aobvious step S2 of reagent drop, reaction 1-3h will be shown made from step S1；
S4, loose colour is washed away, the Sweat latent fingerprint loose colour after step S3 drops show is washed away using ultra-pure water；
S5, finger mark drying, step S4 are washed away the drying of the Sweat latent fingerprint after loose colour；
S6, finger mark observation are fixed, in the case where showing observing system, the Sweat latent fingerprint image after the S5 drying of observation fixing step；
Characterized in that, the noble-metal nanoclusters are in silver nanoclusters, electrum nano-cluster and platinum noble metals nano-cluster
Any one.
3. the method according to claim 2 for showing outmoded Sweat latent fingerprint, it is characterised in that your gold the step S1 prepares
Category nano-cluster shows reagent, including,
The method that silver nanoclusters show reagent is prepared, is comprised the following steps：
(1) solution is prepared, and silver nitrate is dissolved in into the solution that pure water is configured to 0.01mol/L；Bovine serum albumin(BSA) is in 37 DEG C of conditions
Under be dissolved in pure water and be configured to 50mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1mol/L solution；
(2) hybrid reaction, it is by volume 1:1, silver nitrate solution is added into bovine serum albumin solution, by mixed solution in magnetic
3min is reacted in the presence of power agitator；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise into silver nitrate solution obtained by step (2) and bovine serum albumin(BSA) is molten
In the mixed liquor of liquid, regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40~60s of microwave radiation, silver is made
Nano-cluster shows reagent；
The method that gold and silver nano-cluster shows reagent is prepared, is comprised the following steps：
(1) solution is prepared, and gold chloride is dissolved in into the solution that pure water is configured to 0.01mol/L；Silver nitrate is dissolved in pure water and is configured to
0.01mol/L solution；Bovine serum albumin(BSA) is dissolved in pure water under the conditions of 37 DEG C and is configured to 50mg/mL solution；By potassium hydroxide
It is dissolved in pure water and is configured to 1mol/L solution；
(2) hybrid reaction, it is by volume 1:1:2, chlorauric acid solution, silver nitrate solution are added into bovine serum albumin solution,
Mixed solution is reacted into 3min in the presence of magnetic stirring apparatus；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise in the mixed liquor obtained by step (2), and regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40~60s of microwave radiation, silver is made
Nano-cluster shows reagent；
The method that platinum cluster shows reagent is prepared, is comprised the following steps：
(1) solution is prepared, and chloroplatinic acid is dissolved in into the solution that pure water is configured to 0.01mol/L；Bovine serum albumin(BSA) is in 37 DEG C of conditions
Under be dissolved in pure water and be configured to 50mg/mL solution；Potassium hydroxide is dissolved in pure water and is configured to 1mol/L solution；
(2) hybrid reaction, it is by volume 1:1, platinum acid chloride solution is added into bovine serum albumin solution, by mixed solution in magnetic
3min is reacted in the presence of power agitator；
(3) pH value is adjusted, and potassium hydroxide solution is added drop-wise into platinum acid chloride solution obtained by step (2) and bovine serum albumin(BSA) is molten
In the mixed liquor of liquid, regulation pH is 12；
(4) ultrasonic disperse, the mixed solution obtained by step (3) is placed in ultrasonic disperse 1h in ultrasonic washing instrument；
(5) microwave radiation, then the solution obtained by step (4) is placed in micro-wave oven, 40~60s of microwave radiation, silver is made
Nano-cluster shows reagent.
4. the method for showing outmoded Sweat latent fingerprint according to Claims 2 or 3, it is characterised in that the step S2 is restrained
Finger mark, including, the resting period of the finger mark sample of acquisition is 7-30 days.
5. the method for showing outmoded Sweat latent fingerprint according to Claims 2 or 3, it is characterised in that the step S3 drops are aobvious
Finger mark, including, show reagent with the noble-metal nanoclusters obtained by rubber head dropper aspiration step S1, make it along finger mark mastoid process streakline
Drop is aobvious clockwise, and fully reacts 2h with finger mark material.
6. the method for showing outmoded Sweat latent fingerprint according to Claims 2 or 3, it is characterised in that the step S5 finger marks
Drying, including, in baking oven machine vacuum, dry under the conditions of 37 DEG C.
7. the method for showing outmoded Sweat latent fingerprint according to Claims 2 or 3, it is characterised in that the step S6 finger marks
Observation, including, it is to show observation using what alternative light source, colour light filter mirror, digital single-lens reflex camera were built to show observing system
System.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610283026.0A CN105907389B (en) | 2016-05-03 | 2016-05-03 | Noble-metal nanoclusters show the preparation of reagent and show the method for Sweat latent fingerprint |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610283026.0A CN105907389B (en) | 2016-05-03 | 2016-05-03 | Noble-metal nanoclusters show the preparation of reagent and show the method for Sweat latent fingerprint |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105907389A CN105907389A (en) | 2016-08-31 |
| CN105907389B true CN105907389B (en) | 2018-03-27 |
Family
ID=56753153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610283026.0A Expired - Fee Related CN105907389B (en) | 2016-05-03 | 2016-05-03 | Noble-metal nanoclusters show the preparation of reagent and show the method for Sweat latent fingerprint |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105907389B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106891016A (en) * | 2017-01-10 | 2017-06-27 | 青岛科技大学 | A kind of preparation of fluorescence silver nanoclusters and its method for manifesting latent fingerprint |
| CN106995693B (en) * | 2017-04-01 | 2020-03-06 | 西南政法大学 | Method for displaying fingerprint by using fluorescent magnetic precious metal nano cluster core-shell microspheres |
| CN107552809B (en) * | 2017-08-28 | 2019-06-21 | 大连理工大学 | A kind of synthetic method for the color controllable fluorescence gold nanoclusters that silver is adjusted |
| CN108059954A (en) * | 2017-12-13 | 2018-05-22 | 喻彦林 | Composite material shown for latent and preparation method thereof |
| CN108213459B (en) * | 2018-03-14 | 2020-12-29 | 郑州轻工业学院 | Preparation method of glucan/nano gold-silver alloy compound |
| CN109557066B (en) * | 2019-01-25 | 2021-06-15 | 西南政法大学 | Preparation method of copper nano-cluster sweat latent fingerprint developing reagent and sweat latent fingerprint developing method |
| CN109893138B (en) * | 2019-03-04 | 2021-11-02 | 西南政法大学 | Preparation for visually diagnosing diabetes mellitus without wound, and preparation and use methods thereof |
| CN112170859A (en) * | 2020-10-09 | 2021-01-05 | 深圳技术大学 | Preparation method of gold nanocluster |
| CN112175608B (en) * | 2020-10-22 | 2021-11-02 | 江南大学 | Blue fluorescent silver nanocluster and preparation method and application thereof |
| CN113618059A (en) * | 2021-09-03 | 2021-11-09 | 云南民族大学 | Tryptophan-terminated silver nanocluster fluorescent probe and preparation method and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070691B (en) * | 2012-11-09 | 2014-11-05 | 哈尔滨师范大学 | Method for manifesting latent fingerprints on basis of fluorescent gold nanoclusters |
| CN103431867A (en) * | 2013-08-20 | 2013-12-11 | 北京科技大学 | Method for displaying latent fingerprints on fluorogold nano-cluster protected by protein |
| CN103464780B (en) * | 2013-09-06 | 2017-01-25 | 湖南科技大学 | Preparation method of fluorescence gold nano clusters with stable chicken ovalbumin |
-
2016
- 2016-05-03 CN CN201610283026.0A patent/CN105907389B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN105907389A (en) | 2016-08-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105907389B (en) | Noble-metal nanoclusters show the preparation of reagent and show the method for Sweat latent fingerprint | |
| Liu et al. | Carbon dots: synthesis, formation mechanism, fluorescence origin and sensing applications | |
| Moret et al. | Nanoparticles for fingermark detection: an insight into the reaction mechanism | |
| CN105968145B (en) | A kind of metal organic frame fluorescence probe and its application in sulfurated hydrogen detection | |
| CN106885797B (en) | A kind of orientation surface enhancing Raman spectra detection process based on high activity site | |
| Jia et al. | The fabrication of water-stable perovskite-europium hybrid polychromatic fluorescence nanosensor for fast visual sensing of tetracycline | |
| CN103357886A (en) | Preparation method for noble metal nanoclusters of fluorescent sensor | |
| CN103431867A (en) | Method for displaying latent fingerprints on fluorogold nano-cluster protected by protein | |
| CN106957243A (en) | A kind of copper ion detection probe based on aggregation-induced emission and its preparation method and application | |
| CN110724156B (en) | Method for enhancing fluorescence intensity of copper nanocluster | |
| CN106520114B (en) | A kind of preparation method and application of fluorescence gold nano cluster probe | |
| CN111208101B (en) | Method for conveniently detecting aluminum ions based on carbon quantum dots synthesized by flavonoid glycoside and derivatives thereof | |
| CN105328203B (en) | 1 H, 1,2,4 triazoles, 3 mercaptan bovine serum albumin(BSA) fluorescent au nanocluster material and preparation method thereof | |
| CN107138736B (en) | Preparation method and application of aggregation-state phosphorescent copper nanocluster | |
| Sabbarwal et al. | Synthesis of biocompatible, BSA capped fluorescent CaCO 3 pre-nucleation nanoclusters for cell imaging applications | |
| Andrade et al. | Easy preparation of gold nanostructures supported on a thiolated silica-gel for catalysis and latent fingerprint detection | |
| CN105950134A (en) | Material for fabricating high-definition and high-sensitivity identification fingerprints and fingerprint identification method | |
| Zhu et al. | Metal formate framework-assisted solid fluorescent material based on carbonized nanoparticles for the detection of latent fingerprints | |
| CN110018146B (en) | Method for detecting palladium ions based on fluorescent carbon quantum dots | |
| CN109776580B (en) | Silver cluster material with thermochromic luminescence property and preparation method and application thereof | |
| CN114525133A (en) | Preparation and detection of hydrophobic copper nanocluster-containing colloidal solution3+Application of (2) | |
| CN109557066A (en) | Copper nano-cluster Sweat latent fingerprint shows preparation method of reagent thereof and Sweat latent fingerprint process for show | |
| CN109794616A (en) | The preparation of the silver nanoclusters of sericin package and fluorescence probe | |
| CN108558954A (en) | A kind of spin crossover-goes up conversion nano compound material and its preparation method and application | |
| CN108568519A (en) | A kind of preparation method and application of argentum nano composite material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Huang Rui Inventor after: Chen Hongyu Inventor before: Huang Rui Inventor before: Yi Min Inventor before: Chen Hongyu Inventor before: Liu Rui |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180327 Termination date: 20200503 |