CN106891016A - A kind of preparation of fluorescence silver nanoclusters and its method for manifesting latent fingerprint - Google Patents
A kind of preparation of fluorescence silver nanoclusters and its method for manifesting latent fingerprint Download PDFInfo
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- CN106891016A CN106891016A CN201710016028.8A CN201710016028A CN106891016A CN 106891016 A CN106891016 A CN 106891016A CN 201710016028 A CN201710016028 A CN 201710016028A CN 106891016 A CN106891016 A CN 106891016A
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- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 title claims abstract description 62
- 229910052709 silver Inorganic materials 0.000 title claims abstract description 61
- 239000004332 silver Substances 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 8
- 230000008569 process Effects 0.000 claims abstract description 5
- 230000002776 aggregation Effects 0.000 claims abstract description 4
- 238000004220 aggregation Methods 0.000 claims abstract description 4
- 230000005540 biological transmission Effects 0.000 claims abstract description 4
- 238000002189 fluorescence spectrum Methods 0.000 claims abstract description 4
- 150000001413 amino acids Chemical class 0.000 claims abstract description 3
- 230000005284 excitation Effects 0.000 claims abstract description 3
- 239000004519 grease Substances 0.000 claims abstract description 3
- 239000000203 mixture Substances 0.000 claims abstract description 3
- 239000002105 nanoparticle Substances 0.000 claims abstract description 3
- 210000002374 sebum Anatomy 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 59
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 42
- 229960003180 glutathione Drugs 0.000 claims description 21
- 101710134784 Agnoprotein Proteins 0.000 claims description 12
- 108010024636 Glutathione Proteins 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 210000004243 sweat Anatomy 0.000 claims description 11
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 8
- 239000012498 ultrapure water Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 7
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
- 239000010703 silicon Substances 0.000 claims description 5
- 229910052710 silicon Inorganic materials 0.000 claims description 5
- 238000007654 immersion Methods 0.000 claims description 4
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(I) nitrate Inorganic materials [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 230000009189 diving Effects 0.000 claims description 2
- 239000004579 marble Substances 0.000 claims description 2
- 238000012360 testing method Methods 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 235000013339 cereals Nutrition 0.000 claims 1
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- 238000002156 mixing Methods 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 238000001514 detection method Methods 0.000 abstract description 4
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- 239000002994 raw material Substances 0.000 abstract 1
- 239000003643 water by type Substances 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 239000002082 metal nanoparticle Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 230000002498 deadly effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000005357 flat glass Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- -1 sebum Substances 0.000 description 1
- GGCZERPQGJTIQP-UHFFFAOYSA-N sodium;9,10-dioxoanthracene-2-sulfonic acid Chemical compound [Na+].C1=CC=C2C(=O)C3=CC(S(=O)(=O)O)=CC=C3C(=O)C2=C1 GGCZERPQGJTIQP-UHFFFAOYSA-N 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/117—Identification of persons
- A61B5/1171—Identification of persons based on the shapes or appearances of their bodies or parts thereof
- A61B5/1172—Identification of persons based on the shapes or appearances of their bodies or parts thereof using fingerprinting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/05—Metallic powder characterised by the size or surface area of the particles
- B22F1/054—Nanosized particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/06—Metallic powder characterised by the shape of the particles
- B22F1/065—Spherical particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/07—Metallic powder characterised by particles having a nanoscale microstructure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
Abstract
The invention belongs to detection technique field, and in particular to a kind of preparation of fluorescence silver nanoclusters and its method for manifesting latent fingerprint.Characterized by transmission electron microscope, the pattern of fluorescence silver nanoclusters is spheroidal nano particle, and average grain diameter is 2.19nm.The fluorescence spectrum of fluorescence silver nanoclusters is tested, is excited in the excitation wavelength condition of 445nm, silver nanoclusters have strong fluorescent emission at 610nm, and orange fluorescence is sent under uviol lamp 365nm light sources.The suction-operated that the present invention passes through the materials such as amino acid, sebum, the grease in the fluorescence silver nanoclusters and the latent fingerprint composition that prepare, can be more in fingerprint ridge region clustering, forms the concentration aggregation in streakline region.The fingerprint ridge of fingerprint sample is clearly showed under ultraviolet source, so as to reach the purpose that fluorescence manifests latent fingerprint.Raw material argent salt is cheap in the present invention, and material is easily produced, quick, easy to operate, building-up process environmental protection.Sensitivity of the present invention is high, nonhazardous, and fingerprint potential information is not destroyed.
Description
Technical field
The invention belongs to detection technique field, and in particular to the preparation of a kind of fluorescence silver nanoclusters and its for manifesting latent finger
The method of line.
Background technology
Fingerprint has people variant, throughout one's life the characteristics of constant and tactile thing trace.Science correctly find, extract, manifesting and
Identification fingerprint is for opening investigation, punish crime acts on important practice；Fingerprint identification be carry out ten people view it is other most
One of reliable method, is used for investigating, disclose and confirming crime in forensic science；In the investigation and treatment of accident, some
The victim or died of accident or natural calamity, when gesture or other reasonses are with the naked eye beyond recognition due to wound, extract their finger
Line, tends to provide definite identification；Personal finger print data input identity card, credit card and books are borrowed in civil activity
Read in card, can be used to recognize whether registration person is identical with holder；Same application can also be in security protection, safety check and business
Business, such as the unlatching of automatic door lock, go out population management and control of stamping in terms of；In clinical medicine, dermatoglyph can be examination and examine
Disconnected disease provides information and foundation；With the popularization and the application of automatic system of fingerprint recognition of computer technology, fingerprint technique is just
And will be increasingly widely applied in the life of people.
Being applied to the method that fingerprint on site manifests at present mainly has：Optics appearance method, physics appearance method and chemical appearance method.
The common weakness of these methods is low sensitivity, wherein the sensitivity of DNA tests and result are produced after some method applications doing
Disturb.Wherein most widely used method is powder method, but bigger to the physical impairment of professional and technical personnel, and this method
Deadly defect be that outmoded fingerprint can not be manifested.Fluorescence appearance method has special advantage, can show that some are differentiated
The latent fingerprint of unconspicuous background surface, the photoluminescent techniques for using at present are while reasons for its use fluorescence is to the photic of routine
The interference of luminous organism print identification is very big, and difficulty reaches expected requirement.Therefore Non-toxic and high sensitivity live biometric trace
The research and development of appearing technique have turned into the emphasis of forensic science research and the striving direction of countries in the world criminal technique personnel.
Metal nanoparticle has been applied successfully in the diagnosis and treatment of various nano biological sensors and disease, in biology point
There is huge application prospect in son detection and sensing, while metal nanometer cluster also receives much concern, metal nanometre cluster prepares letter
List, uniform particle sizes, stable chemical nature, affinity strong, good biocompatibility, the advantages of be easy to biomolecule fixation and modify, can
Improve the sensitivity that Bio-imprinting manifests.Aobvious is carried out to the latent fingerprint on the object such as paper, sheet glass surface using metal nanoparticle
Now show sensitivity very high, but as the obvious problem of reagent is manifested be cost high due to metal nanoparticle, it is right
Carrying out large-scale use will have that funds are too high.Manifest latent fingerprint therefore, it is possible to quick, highly sensitive, again can
It is the problem that researcher needs to solve by expense reduction.Based on this demand, a kind of copper with photoluminescent property how is researched and developed molten
Glue, can manifest latent fingerprint as reagent is manifested, and manifest process soon and material cost is very low, be suitable to large-scale application, have
Important realistic meaning.
The content of the invention
For problems of the prior art, it is an object of the invention to provide a kind of fluorescence silver nanoclusters preparation and its
Method for manifesting latent fingerprint, with fluorescent both matter silver nanoclusters as manifesting the latent fingerprint of materials exhibit.
The technical scheme that the present invention takes is：
A kind of preparation method of fluorescence silver nanoclusters, specifically includes following steps：
Prepare solution：Under room temperature condition, 8~9mg AgNO are accurately weighed3It is dissolved into 2mL ultra-pure waters, is configured to 20mM
Solution, and weigh 60~65mg glutathione (GSH) powder and it is completely dissolved in 4mL ultra-pure waters and be configured to concentration and be
The solution of 100mM, the solution that will be prepared is stored for future use under the conditions of 4 DEG C；
The preparation of fluorescence silver nanoclusters：To being separately added into 0.5mL 20mM AgNO in round-bottomed flask3Solution and 0.15mL
100mM GSH solution, while adding 4.35mL ultra-pure waters, after making it well mixed, persistently stirs under 50-90 DEG C of heating condition
Stop heating after 12-24h, make solution continue to stir until solution temperature is down to room temperature, you can to obtain fluorescence silver nanoclusters, pass through
Transmission electron microscope is characterized, and the pattern of fluorescence silver nanoclusters is spheroidal nano particle, and average grain diameter is 2.19nm (as shown in Figure 1),
The fluorescence spectrum of silver nanoclusters is tested, is excited in the excitation wavelength condition of 445nm, silver nanoclusters have strong fluorescence at 610nm
Transmitting, sends orange fluorescence (as shown in Figure 2) under uviol lamp 365nm light sources.
Solution preparation is first carried out in first step, solution is abundant in solution state reaction as one of reaction condition, is also
The essential condition that can be reacted.
The application of the preparation method of fluorescence silver nanoclusters, fluorescence silver nanoclusters are used for the method for manifesting latent fingerprint, specific mistake
Journey is as follows：
Prepare the latent fingerprint sample of sweat：Finger is gently pressed in any fingerprint carrier surface such as glass, silicon chip, marble,
Surface can leave potential fingerprint trace, obtain the latent fingerprint sample of sweat；
Latent fingerprint manifests：After the silver nanoclusters that will be prepared dilute 1-10 times, take and drop in rapidly on a small quantity the latent fingerprint sample of sweat
The surface of product, or will be loaded with the object of latent fingerprint immersion silver nanoclusters solution after dilution, silver nanoclusters are due to fingerprint of diving
The suction-operated of the materials such as amino acid, sebum, grease in composition, can be more in fingerprint ridge region clustering, forms streakline area
The concentration aggregation in domain, in 30-50 DEG C of drying, after the silver nanoclusters on sample are all dried, the carrier such as glass, silicon chip is in ultraviolet light
Orange fluorescence is sent under source, the fingerprint ridge of fingerprint sample clearly shows (as shown in Figure 3), shown so as to reach fluorescence
Now the purpose of latent fingerprint, now, capable of taking pictures to carry out leaving and taking finger print data.
The core content of the method is exactly that silver nanoclusters are dripped on fingerprint, it is also possible to take immersion a period of time, ultrasound etc.
Method, as long as the solution of silver nanoclusters is contacted with fingerprint sample, also, after drying, can just show latent fingerprint, be on streakline
Existing fluorescence.
Beneficial effects of the present invention are：
1st, it is sensitive high, quick, easy to operate, building-up process environmental protection.
2nd, synthesis, detection process are without any side effects.
3rd, the potential information of latent fingerprint is not destroyed, does not influence the DNA of latent fingerprint to identify..
4th, metal silver salt is cheap, and material is easily produced.
Brief description of the drawings
Fig. 1：The transmission electron microscope photo of fluorescence silver nanoclusters.
Fig. 2：The fluorescence spectra of fluorescence gold nanoclusters.
Fig. 3：Fluorescence gold nanoclusters manifest latent fingerprint.
Specific embodiment
The present invention is further illustrated below in conjunction with the accompanying drawings.
Glutathione (GSH) is combined by glutamic acid, cysteine and glycine, the tripeptides containing sulfydryl, with anti-
Oxidation and integration detoxication.Glutathione can participate in biotransformation, so as to the poisonous substance conversion being harmful in body
It is harmless material, excretes external.Glutathione can also assist in keeping the function of normal immune system.
A kind of preparation method of fluorescence silver nanoclusters, specifically includes following steps：
Prepare solution：Under room temperature condition, AgNO is accurately weighed3It is dissolved into ultra-pure water, is configured to the solution of 10-30mM,
And weighing glutathione powder makes it be completely dissolved in ultra-pure water, the solution that concentration is 90-110mM is configured to, by what is prepared
Solution is stored for future use under the conditions of 3-5 DEG C；
The preparation of fluorescence silver nanoclusters：It is 10-30mM AgNO to concentration is separately added into round-bottomed flask3Solution, 90-
110mM glutathione solutions and ultra-pure water, AgNO3The volume ratio 1 of solution, glutathione solution and ultra-pure water:3:(8-9), makes
After it is well mixed, heating is stopped after persistently stirring 12-24h under 50-90 DEG C of heating condition, solution continues to stir until solution
Temperature is down to room temperature, you can obtain fluorescence silver nanoclusters.
Embodiment 1
Prepare solution：Under room temperature condition, 8~9mg AgNO are accurately weighed3It is dissolved into 2mL ultra-pure waters, is configured to 20mM
Solution, and weigh 60~65mg glutathione (GSH) powder and it is completely dissolved in 4mL ultra-pure waters and be configured to concentration and be
The solution of 100mM, the solution that will be prepared is stored for future use under the conditions of 4 DEG C.
The preparation of fluorescence silver nanoclusters：To being separately added into 0.5mL 20mM AgNO in round-bottomed flask3Solution and 0.15mL
100mM GSH solution, while 4.35mL ultra-pure waters are added, after making it well mixed, after persistently stirring 24h under 70 DEG C of heating conditions
Stop heating, make solution continue to stir until solution temperature is down to room temperature, you can to obtain fluorescence silver nanoclusters, swash in ultraviolet source
Fluorescent orange (as shown in Figure 2) is sent under conditions of hair.
Embodiment 2
Prepare solution：Under room temperature condition, 8~9mg AgNO are accurately weighed3It is dissolved into 2mL ultra-pure waters, is configured to 20mM
Solution, and weigh 60~65mg glutathione (GSH) powder and it is completely dissolved in 4mL ultra-pure waters and be configured to concentration and be
The solution of 100mM, the solution that will be prepared is stored for future use under the conditions of 4 DEG C.
The preparation of fluorescence silver nanoclusters：To being separately added into 0.5mL 20mM AgNO in round-bottomed flask3Solution and 0.15mL
100mM GSH solution, while 4.35mL ultra-pure waters are added, after making it well mixed, after persistently stirring 24h under 50 DEG C of heating conditions
Stop heating, make solution continue to stir until solution temperature is down to room temperature, you can to obtain fluorescence silver nanoclusters, swash in ultraviolet source
Fluorescent orange (as shown in Figure 2) is sent under conditions of hair.
Embodiment 3
Prepare solution：Under room temperature condition, 8~9mg AgNO are accurately weighed3It is dissolved into 2mL ultra-pure waters, is configured to 20mM
Solution, and weigh 60~65mg glutathione (GSH) powder and it is completely dissolved in 4mL ultra-pure waters and be configured to concentration and be
The solution of 100mM, the solution that will be prepared is stored for future use under the conditions of 4 DEG C.
The preparation of fluorescence silver nanoclusters：To being separately added into 0.5mL 20mM AgNO in round-bottomed flask3Solution and 0.15mL
100mM GSH solution, while 4.35mL ultra-pure waters are added, after making it well mixed, after persistently stirring 12h under 90 DEG C of heating conditions
Stop heating, make solution continue to stir until solution temperature is down to room temperature, you can to obtain fluorescence silver nanoclusters, swash in ultraviolet source
Fluorescent orange (as shown in Figure 2) is sent under conditions of hair.
Embodiment 4
The application of the preparation method of fluorescence silver nanoclusters, fluorescence silver nanoclusters are used for the method for manifesting latent fingerprint, specific mistake
Journey is as follows：
Prepare the latent fingerprint sample of sweat：Finger is gently pressed in glass fingerprint carrier surface, surface can leave and potentially refer to
Escutcheon mark, obtains the latent fingerprint sample of sweat.
Latent fingerprint manifests：After the silver nanoclusters that will be prepared dilute 1-10 times, take and drop in rapidly on a small quantity the latent fingerprint sample of sweat
The surface of product, in 30-50 DEG C of drying, after the silver nanoclusters on sample are all dried, glass fingerprint carrier is issued in ultraviolet source
Go out orange fluorescence, the fingerprint ridge of fingerprint sample is clearly showed, so as to reach the purpose that fluorescence manifests latent fingerprint, this
When, it is capable of taking pictures to carry out leaving and taking finger print data (as shown in Fig. 3 left figures).
Embodiment 5
The application of the preparation method of fluorescence silver nanoclusters, fluorescence silver nanoclusters are used for the method for manifesting latent fingerprint, specific mistake
Journey is as follows：
Prepare the latent fingerprint sample of sweat：Finger is gently pressed in silicon chip fingerprint carrier surface, surface can leave and potentially refer to
Escutcheon mark, obtains the latent fingerprint sample of sweat.
Latent fingerprint manifests：After the silver nanoclusters that will be prepared dilute 1-10 times, the fingerprint carrier immersion of latent fingerprint will be loaded with
In silver nanoclusters solution after dilution, in 30-50 DEG C of drying, after the silver nanoclusters on sample are all dried, silicon chip fingerprint is carried
Body sends orange fluorescence under ultraviolet source, and the fingerprint ridge of fingerprint sample is clearly showed, and shows so as to reach fluorescence
Now the purpose of latent fingerprint, now, capable of taking pictures to carry out leaving and taking finger print data (as shown in Fig. 3 right figures).
The above is not limitation of the present invention, it should be pointed out that：Come for those skilled in the art
Say, on the premise of essential scope of the present invention is not departed from, some changes, remodeling, addition can also be made or replaced, these improvement
Protection scope of the present invention is also should be regarded as with retouching.
Claims (7)
1. a kind of preparation method of fluorescence silver nanoclusters, it is characterised in that specifically include following steps：
Prepare solution：Under room temperature condition, AgNO is accurately weighed3It is dissolved into ultra-pure water, is configured to the solution of 10-30mM, and weigh
Glutathione powder makes it be completely dissolved in ultra-pure water, is configured to the solution that concentration is 90-110mM, and the solution that will be prepared exists
Stored for future use under the conditions of 3-5 DEG C；
The preparation of fluorescence silver nanoclusters：It is 10-30mM AgNO to concentration is separately added into round-bottomed flask3Solution, 90-110mM paddy
The sweet peptide solution of Guang and ultra-pure water, AgNO3The volume ratio 1 of solution, glutathione solution and ultra-pure water:3:(8-9), makes its mixing equal
After even, heating is stopped after persistently stirring 12-24h under 50-90 DEG C of heating condition, solution continues to stir until solution temperature is down to
Room temperature, you can obtain fluorescence silver nanoclusters.
2. a kind of preparation method of fluorescence silver nanoclusters according to claim 1, it is characterised in that resulting fluorescence silver is received
Rice cluster is characterized by transmission electron microscope, and the pattern of fluorescence silver nanoclusters is spheroidal nano particle, and average grain diameter is 2.19nm.
3. a kind of preparation method of fluorescence silver nanoclusters according to claim 1, it is characterised in that test fluorescence silver nanoclusters
Fluorescence spectrum, excited in the excitation wavelength condition of 445nm, silver nanoclusters have strong fluorescent emission at 610nm, in uviol lamp
Orange fluorescence is sent under 365nm light sources.
4. the application of the preparation method of fluorescence silver nanoclusters according to claim 1, it is characterised in that fluorescence silver nanoclusters are used
In the method for manifesting latent fingerprint, detailed process is as follows：
Prepare the latent fingerprint sample of sweat：Finger is gently pressed in fingerprint carrier surface, surface can leave potential fingerprint trace, obtain
To the latent fingerprint sample of sweat；
Latent fingerprint manifests：After the silver nanoclusters that will be prepared dilute 1-10 times, take and drop in the latent fingerprint sample of sweat rapidly on a small quantity
Surface, silver nanoclusters by the amino acid in the fingerprint composition of diving, sebum, each material of grease suction-operated, in fingerprint ridge area
Domain aggregation is more, forms the concentration aggregation in streakline region, in 30-50 DEG C of drying, after the silver nanoclusters on sample are all dried, refers to
Line carrier sends orange fluorescence under ultraviolet source, and the fingerprint ridge of fingerprint sample is clearly showed.
5. the application of the preparation method of fluorescence silver nanoclusters according to claim 4, it is characterised in that the fingerprint carrier choosing
It is selected as any article of glass, silicon chip, marble.
6. the application of the preparation method of fluorescence silver nanoclusters according to claim 4, it is characterised in that the latent fingerprint it is aobvious
Now processing procedure is：To be loaded with the fingerprint carrier of latent fingerprint immersion silver nanoclusters solution after dilution.
7. the application of the preparation method of fluorescence silver nanoclusters according to claim 4, it is characterised in that clearly show
Fingerprint carries out leaving and taking finger print data using shooting style.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710016028.8A CN106891016A (en) | 2017-01-10 | 2017-01-10 | A kind of preparation of fluorescence silver nanoclusters and its method for manifesting latent fingerprint |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710016028.8A CN106891016A (en) | 2017-01-10 | 2017-01-10 | A kind of preparation of fluorescence silver nanoclusters and its method for manifesting latent fingerprint |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106891016A true CN106891016A (en) | 2017-06-27 |
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