CN105733032A - Preparation method and application of silver nanocluster gel - Google Patents

Preparation method and application of silver nanocluster gel Download PDF

Info

Publication number
CN105733032A
CN105733032A CN201610107128.7A CN201610107128A CN105733032A CN 105733032 A CN105733032 A CN 105733032A CN 201610107128 A CN201610107128 A CN 201610107128A CN 105733032 A CN105733032 A CN 105733032A
Authority
CN
China
Prior art keywords
silver nanoclusters
gel
solution
preparation
silver
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610107128.7A
Other languages
Chinese (zh)
Other versions
CN105733032B (en
Inventor
熊华玉
梁继超
王薇
王升富
文为
张修华
郑慧铃
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hubei University
Original Assignee
Hubei University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hubei University filed Critical Hubei University
Priority to CN201610107128.7A priority Critical patent/CN105733032B/en
Publication of CN105733032A publication Critical patent/CN105733032A/en
Application granted granted Critical
Publication of CN105733032B publication Critical patent/CN105733032B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/12—Agar or agar-agar, i.e. mixture of agarose and agaropectin; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00—Processes of treating or compounding macromolecular substances
    • C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
    • C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
    • C08J3/075—Macromolecular gels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
    • C08J2305/12—Agar-agar; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
    • C08J2405/04—Alginic acid; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K2201/00—Specific properties of additives
    • C08K2201/011—Nanostructured additives
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2205/00—Polymer mixtures characterised by other features
    • C08L2205/02—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2205/00—Polymer mixtures characterised by other features
    • C08L2205/03—Polymer mixtures characterised by other features containing three or more polymers in a blend

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Optics & Photonics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Preparation (AREA)
  • Manufacturing Of Micro-Capsules (AREA)

Abstract

The invention provides a preparation method of silver nanocluster gel. The prepared gel is uniform and high in reproducibility; the preparation method is simple, easy and rapid, materials are extensive in resource and low in cost, and a preparation process is pollution-free, and can be used for large-scale production; the prepared silver nanocluster gel is sensitive to a pH value, the fluorescence intensity is enhanced along with increase of the pH, and the silver nanocluster gel is non-fluorescent in a buffer solution with pH of 4-5, so that the silver nanocluster gel can be used for indicating changes of the pH of a system as a fluorescent switch.

Description

The preparation method and applications of silver nanoclusters gel
Technical field
The present invention relates to hydrogel synthetic technology, particularly relate to the preparation method of a kind of silver nanoclusters gel and answer With.
Background technology
The change of pH value is the important mark weighing living organism physiological change, with modern medicine, biological engineering Closely bound up Deng field of scientific study.The method of the change measuring pH value is a lot, such as acidometer, fluorescent material Deng.Fluorimetry uses the change instruction pH value of fluorescence parameter, easy, quick, sensitivity is good, exploitation For detecting living things system H+The pH fluorescent probe of change has wide application in terms of biomedical research Prospect.
Gel is the three-dimensional net structure of crosslinking, typically swelling by the certain solvent of polymeric absorbent prepares, tool Have environment such as temperature, pH value, the characteristic of particular chemicals sensitivity.When by environmental stimuli, gel The conformation of network can have greatly changed.Hydrogel sensitive for pH typically has the acid such as carboxylic acid group, sulfonic group Property group, when pH value changes, gel is because of proton translocation, and changes in certain effect power lower volume Become, thus cause some characteristic changing of gel.Synthesis patent currently for silver nanoclusters gel has CN105193706A " a kind of pH responsive type carries doxorubicin hydrochloride silver nanoclusters hydrogel and application thereof ", this is special Profit is that doxorubicin hydrochloride powder joins silver nanoclusters dispersion liquid, synthesizes in autoclave and noble gas PH responsive type carry doxorubicin hydrochloride hydrogel.This patented technology mainly uses silver nanoclusters hydrogel as load Body, it is achieved medicine slowly discharges." a kind of pH is quick to have CN104893010A for the synthesis patent of hydrogel The preparation method of sense type hydrogel ", this patent is sodium alginate, gelatin, collagen protein of fish skin, agar to be mixed Conjunction is scattered in distilled water, adds calcium carbonate and prepares as gellant.This hydrogel may indicate that pH value Change, does not but have fluorescence display.
Summary of the invention
It is an object of the invention to provide a kind of to pH sensitive, increase along with pH fluorescence intensity strengthen silver The preparation method and applications of nano-cluster gel.
In order to achieve the above object, on the one hand, the invention provides the preparation method of a kind of silver nanoclusters gel, It comprises the following steps:
Step 1, with solvable silver salt, solvable BH4 -Salt, and bovine serum albumin, thioctic acid or gluathione Any one in peptide is raw material, prepares silver nanoclusters solution；
Step 2, joins in deionized water after agarose, sodium alginate and gelatin mix homogeneously, heating, It is made to be completely dissolved formation polysaccharide solution；
Step 3, after the polysaccharide solution obtained until step 2 is cooled to 50-80 DEG C, adds what step 1 obtained Silver nanoclusters solution, stirs, and prepares silver nanoclusters polysaccharide solution, stands, and washing obtains silver nanoclusters Gel.
On the other hand, the silver nanoclusters gel using method described in first aspect present invention to prepare, it is applied to Detection living things system H+The pH fluorescent probe of change.
The invention has the beneficial effects as follows: the preparation method of silver nanoclusters gel provided by the present invention, prepare Gel is more uniform, favorable reproducibility；Preparation method is simple, and quickly, material source is extensively cheap, preparation Process is pollution-free, can be used for large-scale industrial production；Silver nanoclusters gel prepared by the present invention, to pH Value increase fluorescence intensity sensitive, along with pH strengthens, and in the buffer that pH is 4-5, silver nanoclusters coagulates Glue does not has fluorescence, shows that silver nanoclusters gel can be as the change of pH in fluorescent switch directive system.
Accompanying drawing explanation
Fig. 1 is the fluorescence emission spectrogram of the silver nanoclusters gel that the embodiment of the present invention 1 prepares；
Fig. 2 is the silver nanoclusters gel for preparing of the embodiment of the present invention 2 fluorescence under different pH condition Image.
Detailed description of the invention
On the one hand, the invention provides the preparation method of a kind of silver nanoclusters gel, it comprises the following steps:
Step 1, with solvable silver salt, solvable BH4 -Salt, and bovine serum albumin, thioctic acid or glutathion In any one be raw material, prepare silver nanoclusters solution；
Step 2, joins in deionized water after agarose, sodium alginate and gelatin mix homogeneously, heating, It is made to be completely dissolved formation polysaccharide solution；
Step 3, after the polysaccharide solution obtained until step 2 is cooled to 50-80 DEG C, adds what step 1 obtained Silver nanoclusters solution, stirs, and prepares silver nanoclusters polysaccharide solution, stands, and washing obtains silver nanoclusters Gel.
Preferably, described step 1 comprises the following steps:
Step 1-1, any one in configuration BSA, thioctic acid or glutathion is water-soluble with the mixing of solvable silver salt Liquid, drips solvable BH under agitation4 -Saline solution, continues stirring and obtains silver nanoclusters stock solution；
Step 1-2, silver nanoclusters stock solution step 1-1 obtained is dialysed in bag filter, and to obtain silver nanoclusters molten Liquid.
It is further preferred that arbitrary in bovine serum albumin, thioctic acid or glutathion in described step 1-1 Plant and Ag+Mol ratio is 12-16:1.
It is further preferred that Ag in described step 1-1+With BH4 -Mol ratio is 1:4-6.
It is further preferred that continuing mixing time in described step 1-1 is 6-12 hour.
It is further preferred that dialysis time is two days two nights in described step 1-2, centre is changed ultrapure in every three hours Water.
Preferably, in described step 2, agarose, sodium alginate and gelatin weight ratio are 5-9:1-3:0.5-2, add Entering the polysaccharide solution mass concentration obtained in deionized water is 0.1%-1.3%.
Preferably, in described step 3, silver nanoclusters solution and polysaccharide solution volume ratio are 1:1-3.
Preferably, in described step 3, quiescent time is 10-60 minute, uses ultra-pure water cleaning 3-5 time, often Secondary consumption 3-10ml.
On the other hand, the silver nanoclusters gel using method described in first aspect present invention to prepare, it is applied to Detection living things system H+The pH fluorescent probe of change.
Below in conjunction with specific embodiment, the preparation method of silver nanoclusters gel of the present invention is made furtherly Bright.
Embodiment 1
(1) bovine serum albumin dissolving is formed in aqueous the solution of clear；
(2) by AgNO3According to bovine serum albumin: Ag+Mol ratio is that 14.75:1 adds in solution, mixed Close uniformly, form mixed material；
(3) under stirring, Ag in molar ratio+:NaBH4Ratio for 1:4.8 is slowly added dropwise hydroboration Sodium solution, in mixed material, continues 6 hours prepared silver nanoclusters stock solution of stirring；
(4) stock solution is dialysed two days two nights in the bag filter that molecular cut off is 500, middle every three little Time change ultra-pure water, prepare silver nanoclusters solution；
(5) it is 5:3:2 mix homogeneously by agarose, sodium alginate and gelatin according to weight ratio, then by 0.5% (w/v) mixed-powder joins in deionized water, heating so that it is be completely dissolved formation polysaccharide solution, Stop heating；
(6) after polysaccharide solution is cooled to 60 degree, it is that silver nanoclusters solution is added by 0.5:1 according to volume ratio Enter in polysaccharide solution, stir, prepare silver nanoclusters polysaccharide solution；
(7) while hot silver nanoclusters polysaccharide mixed solution is poured in mould, static placement 30 minutes, use Ultra-pure water cleans 4 times, and each consumption 5ml i.e. prepares silver nanoclusters gel.
Embodiment 2
(1) thioctic acid dissolving is formed in aqueous the solution of clear；
(2) by AgNO3According to thioctic acid: Ag+Mol ratio is that 15:1 adds in solution, mix homogeneously, Form mixed material；
(3) under stirring, Ag in molar ratio+:NaBH4Ratio for 1:5 is slowly added dropwise hydroboration Sodium solution, in mixed material, continues 7 hours prepared silver nanoclusters stock solution of stirring；
(4) stock solution is dialysed two days two nights in the bag filter that molecular cut off is 500, middle every three little Time change ultra-pure water, prepare silver nanoclusters solution；
(5) it is 6:3:1 mix homogeneously by agarose, sodium alginate and gelatin according to weight ratio, then by 1% (w/v) Mixed-powder join in deionized water, heating so that it is be completely dissolved formation polysaccharide solution, stop heating；
(6) after polysaccharide solution is cooled to 70 degree, it is that silver nanoclusters solution is added by 1:1 according to volume ratio In polysaccharide solution, stir, prepare silver nanoclusters polysaccharide solution；
(7) while hot silver nanoclusters polysaccharide mixed solution is poured in mould, static placement 20 minutes, use Ultra-pure water cleans 3 times, and each consumption 8ml i.e. prepares silver nanoclusters gel.
Embodiment 3
(1) glutathion dissolving is formed in aqueous the solution of clear；
(2) by AgNO3According to glutathion: Ag+Mol ratio is that 15.5:1 adds in solution, and mixing is all Even, form mixed material；
(3) under stirring, Ag in molar ratio+:NaBH4Ratio for 1:5.5 is slowly added dropwise hydroboration Sodium solution, in mixed material, continues 8 hours prepared silver nanoclusters stock solution of stirring；
(4) stock solution is dialysed two days two nights in the bag filter that molecular cut off is 500, middle every three little Time change ultra-pure water, prepare silver nanoclusters solution；
(5) it is 7:2:1 mix homogeneously by agarose, sodium alginate and gelatin according to weight ratio, then by 0.5% (w/v) mixed-powder joins in deionized water, heating so that it is be completely dissolved formation polysaccharide solution, Stop heating；
(6) after polysaccharide solution is cooled to 60 degree, it is that silver nanoclusters solution is added by 1:2 according to volume ratio In polysaccharide solution, stir, prepare silver nanoclusters polysaccharide solution；
(7) pouring in mould by silver nanoclusters polysaccharide mixed solution while hot, static placement 30 minutes, with super Pure water cleans 5 times, and each consumption 3ml i.e. prepares silver nanoclusters gel.
Embodiment 4
(1) thioctic acid dissolving is formed in aqueous the solution of clear；
(2) by AgNO3According to thioctic acid: Ag+Mol ratio is that 16:1 adds in solution, mix homogeneously, Form mixed material；
(3) under stirring, Ag in molar ratio+:NaBH4Ratio for 1:6 is slowly added dropwise hydroboration Sodium solution, in mixed material, continues 9 hours prepared silver nanoclusters stock solution of stirring；
(4) stock solution is dialysed two days two nights in the bag filter that molecular cut off is 500, middle every three little Time change ultra-pure water, prepare silver nanoclusters solution；
(5) it is 8:1:1 mix homogeneously by agarose, sodium alginate and gelatin according to weight ratio, then by 1% (w/v) Mixed-powder join in deionized water, heating so that it is be completely dissolved formation polysaccharide solution, stop heating；
(6) after polysaccharide solution is cooled to 65 degree, it is that silver nanoclusters solution is added by 1:2 according to volume ratio In polysaccharide solution, stir, prepare silver nanoclusters polysaccharide solution；
(7) pouring in mould by silver nanoclusters polysaccharide mixed solution while hot, static placement 60 minutes, with super Pure water cleans 5 times, and each consumption 5ml i.e. prepares silver nanoclusters gel.
Embodiment 5
(1) bovine serum albumin dissolving is formed in aqueous the solution of clear；
(2) by AgNO3According to bovine serum albumin: Ag+Mol ratio is that 14.5:1 adds in solution, mixed Close uniformly, form mixed material；
(3) under stirring, Ag in molar ratio+:NaBH4Ratio for 1:4.5 is slowly added dropwise hydroboration Sodium solution, in mixed material, continues 10 hours prepared silver nanoclusters stock solution of stirring；
(4) stock solution is dialysed two days two nights in the bag filter that molecular cut off is 500, middle every three little Time change ultra-pure water, prepare silver nanoclusters solution；
(5) it is 9:1:0.5 mix homogeneously by agarose, sodium alginate and gelatin according to weight ratio, then by 0.6% (w/v) mixed-powder joins in deionized water, heating so that it is be completely dissolved formation polysaccharide solution, Stop heating；
(6) after polysaccharide solution is cooled to 60 degree, it is that silver nanoclusters solution is added by 1:3 according to volume ratio In polysaccharide solution, stir, prepare silver nanoclusters polysaccharide solution；
(7) pouring in mould by silver nanoclusters polysaccharide mixed solution while hot, static placement 30 minutes, with super Pure water cleans 3 times, and each consumption 4ml i.e. prepares silver nanoclusters gel.
Embodiment 6
(1) glutathion dissolving is formed in aqueous the solution of clear；
(2) by AgNO3According to glutathion: Ag+Mol ratio is that 14:1 adds in solution, mix homogeneously, Form mixed material；
(3) under stirring, Ag in molar ratio+:NaBH4Ratio for 1:4 is slowly added dropwise hydroboration Sodium solution, in mixed material, continues 11 hours prepared silver nanoclusters stock solution of stirring；
(4) stock solution is dialysed two days two nights in the bag filter that molecular cut off is 500, middle every three little Time change ultra-pure water, prepare silver nanoclusters solution；
(5) it is 8:1.5:0.5 mix homogeneously by agarose, sodium alginate and gelatin according to weight ratio, then will The mixed-powder of 1.2% (w/v) joins in deionized water, heating so that it is be completely dissolved formation polysaccharide molten Liquid, stops heating；
(6) after polysaccharide solution is cooled to 75 degree, it is that silver nanoclusters solution is added by 1:3 according to volume ratio In polysaccharide solution, stir, prepare silver nanoclusters polysaccharide solution；
(7) pouring in mould by silver nanoclusters polysaccharide mixed solution while hot, static placement 30 minutes, with super Pure water cleans 4 times, and each consumption 5ml i.e. prepares silver nanoclusters gel.
Embodiment 7
(1) glutathion dissolving is formed in aqueous the solution of clear；
(2) by AgNO3According to glutathion: Ag+Mol ratio is that 13:1 adds in solution, mix homogeneously, Form mixed material；
(3) under stirring, Ag in molar ratio+:NaBH4Ratio for 1:4 is slowly added dropwise hydroboration Sodium solution, in mixed material, continues 12 hours prepared silver nanoclusters stock solution of stirring；
(4) stock solution is dialysed two days two nights in the bag filter that molecular cut off is 500, middle every three little Time change ultra-pure water, prepare silver nanoclusters solution；
(5) it is 8:0.5:1.5 mix homogeneously by agarose, sodium alginate and gelatin according to weight ratio, then will The mixed-powder of 0.9% (w/v) joins in deionized water, heating so that it is be completely dissolved formation polysaccharide molten Liquid, stops heating；
(6) after polysaccharide solution is cooled to 70 degree, it is that silver nanoclusters solution is added by 1:1 according to volume ratio In polysaccharide solution, stir, prepare silver nanoclusters polysaccharide solution；
(7) pouring in mould by silver nanoclusters polysaccharide mixed solution while hot, static placement 40 minutes, with super Pure water cleans 3 times, and each consumption 5ml i.e. prepares silver nanoclusters gel.
Embodiment 8
(1) glutathion dissolving is formed in aqueous the solution of clear；
(2) by AgNO3According to glutathion: Ag+Mol ratio is that 12:1 adds in solution, mix homogeneously, Form mixed material；
(3) under stirring, Ag in molar ratio+:NaBH4Ratio for 1:4 is slowly added dropwise hydroboration Sodium solution, in mixed material, continues 12 hours prepared silver nanoclusters stock solution of stirring；
(4) stock solution is dialysed two days two nights in the bag filter that molecular cut off is 500, middle every three little Time change ultra-pure water, prepare silver nanoclusters solution；
(5) it is 7:2:1 mix homogeneously by agarose, sodium alginate and gelatin according to weight ratio, then by 1.3% (w/v) mixed-powder joins in deionized water, heating so that it is be completely dissolved formation polysaccharide solution, Stop heating；
(6) after polysaccharide solution is cooled to 70 degree, it is that silver nanoclusters solution is added by 1:2 according to volume ratio In polysaccharide solution, stir, prepare silver nanoclusters polysaccharide solution；
(7) while hot silver nanoclusters polysaccharide mixed solution is poured in mould, static placement 40 minutes, use Ultra-pure water cleans 4 times, and each consumption 4ml i.e. prepares silver nanoclusters gel.
The fluorescence spectrum of the silver nanoclusters gel at room temperature test solution obtaining embodiment 1, obtains Fig. 1 Shown result, as shown in Figure 1, prepared bunch solution has good fluorescence emission spectrum.
After the silver nanoclusters gel obtaining embodiment 2 soaks 2 hours in different ph values buffer, with solidifying Glue imager is taken a picture, and obtains the result shown in Fig. 2, as shown in Figure 2, under the exciting of ultraviolet, different Gel under the conditions of ph value presents different fluorescence intensities.
The foregoing is only the better embodiment of the present invention, not in order to limit invention, all essences in the present invention Within god and principle, any modification, equivalent substitution and improvement etc. done, should be included in the protection of the present invention Within the scope of.

Claims (10)

1. the preparation method of a silver nanoclusters gel, it is characterised in that: it comprises the following steps:
Step 1, with solvable silver salt, solvable BH4 -Salt, and bovine serum albumin, thioctic acid or gluathione Any one in peptide is raw material, prepares silver nanoclusters solution；
Step 2, joins in deionized water after agarose, sodium alginate and gelatin mix homogeneously, heating, It is made to be completely dissolved formation polysaccharide solution；
Step 3, after the polysaccharide solution obtained until step 2 is cooled to 50-80 DEG C, adds what step 1 obtained Silver nanoclusters solution, stirs, and prepares silver nanoclusters polysaccharide solution, stands, and washing obtains silver nanoclusters Gel.
2. the preparation method of silver nanoclusters gel as claimed in claim 1, it is characterised in that: described step 1 is wrapped Include following steps:
Step 1-1, any one in configuration bovine serum albumin, thioctic acid or glutathion and solvable silver salt Mixed aqueous solution, drips solvable BH under agitation4 -Saline solution, continues stirring and obtains silver nanoclusters stock solution；
Step 1-2, silver nanoclusters stock solution step 1-1 obtained is dialysed in bag filter, and to obtain silver nanoclusters molten Liquid.
3. the preparation method of silver nanoclusters gel as claimed in claim 2, it is characterised in that: described step 1-1 Any one in middle bovine serum albumin, thioctic acid or glutathion and Ag+Mol ratio is 12-16:1.
4. the preparation method of silver nanoclusters gel as claimed in claim 2, it is characterised in that: described step 1-1 Middle Ag+With BH4 -Mol ratio is 1:4-6.
5. the preparation method of silver nanoclusters gel as claimed in claim 2, it is characterised in that: described step 1-1 Middle continuation mixing time is 6-12 hour.
6. the preparation method of silver nanoclusters gel as claimed in claim 2, it is characterised in that: described step 1-2 Middle dialysis time is two days two nights, and ultra-pure water is changed in every three hours in centre.
7. the preparation method of silver nanoclusters gel as claimed in claim 1, it is characterised in that: in described step 2 Agarose, sodium alginate and gelatin weight ratio are 5-9:1-3:0.5-2, join the poly obtained in deionized water Sugar juice mass concentration is 0.1%-1.3%.
8. the preparation method of silver nanoclusters gel as claimed in claim 1, it is characterised in that: in described step 3 Silver nanoclusters solution and polysaccharide solution volume ratio are 1:1-3.
9. the preparation method of silver nanoclusters gel as claimed in claim 1, it is characterised in that: in described step 3 Quiescent time is 10-60 minute, uses ultra-pure water to clean 3-5 time, each consumption 3-10ml.
10. the silver nanoclusters gel using method described in claim 1 to prepare, is applied to detect organism It is H+The pH fluorescent probe of change.
CN201610107128.7A 2016-02-26 2016-02-26 The preparation method and applications of silver nanoclusters gel Expired - Fee Related CN105733032B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610107128.7A CN105733032B (en) 2016-02-26 2016-02-26 The preparation method and applications of silver nanoclusters gel

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610107128.7A CN105733032B (en) 2016-02-26 2016-02-26 The preparation method and applications of silver nanoclusters gel

Publications (2)

Publication Number Publication Date
CN105733032A true CN105733032A (en) 2016-07-06
CN105733032B CN105733032B (en) 2018-01-26

Family

ID=56248561

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610107128.7A Expired - Fee Related CN105733032B (en) 2016-02-26 2016-02-26 The preparation method and applications of silver nanoclusters gel

Country Status (1)

Country Link
CN (1) CN105733032B (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106891016A (en) * 2017-01-10 2017-06-27 青岛科技大学 A kind of preparation of fluorescence silver nanoclusters and its method for manifesting latent fingerprint
CN107596436A (en) * 2017-09-26 2018-01-19 天津大学 A kind of DNA fluorescence hydrogel and preparation method thereof
CN111035801A (en) * 2020-01-14 2020-04-21 青岛科技大学 Silver nanocluster based chitosan hydrogel dressing and preparation method and application thereof
CN111228302A (en) * 2020-02-27 2020-06-05 青岛科技大学 Antibacterial hydrogel and preparation method and application thereof
CN111739998A (en) * 2020-07-03 2020-10-02 青岛科技大学 High-color-rendering white light LED based on silver clusters and preparation method thereof
CN115216108A (en) * 2022-06-21 2022-10-21 清华-伯克利深圳学院筹备办公室 Hydrogel and preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103314073A (en) * 2010-12-30 2013-09-18 印度马德拉斯理工学院 Gold and silver quantum clusters and methods for their preparation and use
CN103827035A (en) * 2011-10-28 2014-05-28 印度马德拉斯理工学院 Methods of preparing metal quantum clusters in molecular confinement
CN105193706A (en) * 2015-11-03 2015-12-30 江苏康呵健康科技有限公司 PH-sensitive doxorubicin hydrochloride loaded silver nano-cluster hydrogel and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103314073A (en) * 2010-12-30 2013-09-18 印度马德拉斯理工学院 Gold and silver quantum clusters and methods for their preparation and use
CN103827035A (en) * 2011-10-28 2014-05-28 印度马德拉斯理工学院 Methods of preparing metal quantum clusters in molecular confinement
CN105193706A (en) * 2015-11-03 2015-12-30 江苏康呵健康科技有限公司 PH-sensitive doxorubicin hydrochloride loaded silver nano-cluster hydrogel and application thereof

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106891016A (en) * 2017-01-10 2017-06-27 青岛科技大学 A kind of preparation of fluorescence silver nanoclusters and its method for manifesting latent fingerprint
CN107596436A (en) * 2017-09-26 2018-01-19 天津大学 A kind of DNA fluorescence hydrogel and preparation method thereof
CN111035801A (en) * 2020-01-14 2020-04-21 青岛科技大学 Silver nanocluster based chitosan hydrogel dressing and preparation method and application thereof
CN111035801B (en) * 2020-01-14 2022-01-14 青岛科技大学 Silver nanocluster based chitosan hydrogel dressing and preparation method and application thereof
CN111228302A (en) * 2020-02-27 2020-06-05 青岛科技大学 Antibacterial hydrogel and preparation method and application thereof
CN111739998A (en) * 2020-07-03 2020-10-02 青岛科技大学 High-color-rendering white light LED based on silver clusters and preparation method thereof
CN115216108A (en) * 2022-06-21 2022-10-21 清华-伯克利深圳学院筹备办公室 Hydrogel and preparation method and application thereof

Also Published As

Publication number Publication date
CN105733032B (en) 2018-01-26

Similar Documents

Publication Publication Date Title
CN105733032A (en) Preparation method and application of silver nanocluster gel
CN103357886B (en) A kind of preparation method of the noble metal nano cluster for fluorescent optical sensor
Shen et al. A self-healing carboxymethyl chitosan/oxidized carboxymethyl cellulose hydrogel with fluorescent bioprobes for glucose detection
CN102408496B (en) In-situ crosslinked alginate hydrogels and preparation method thereof
Khattab et al. Co-encapsulation of enzyme and tricyanofuran hydrazone into alginate microcapsules incorporated onto cotton fabric as a biosensor for colorimetric recognition of urea
CN105651774B (en) A kind of fluorescence nano gold cluster gel and preparation method thereof
Traffano-Schiffo et al. Gums induced microstructure stability in Ca (II)-alginate beads containing lactase analyzed by SAXS
CN103102512B (en) Chitosan-fullerene compound and preparation method
US20210023770A1 (en) Cell Electrochemical Sensor Based on 3D Printing Technology and Application Thereof
Billah et al. Quality maintenance of fish farm: development of real-time water quality monitoring system
Yang et al. Hydrogel-derived luminescent scaffolds for biomedical applications
Tavakoli et al. AIEgen quantitatively monitoring the release of Ca2+ during swelling and degradation process in alginate hydrogels
CN104897868A (en) Six-in-one test paper for fast detecting quality of water in aquarium
Rizzo et al. A pH-sensor scaffold for mapping spatiotemporal gradients in three-dimensional in vitro tumour models
ES2252965T3 (en) PROCEDURE FOR OBTAINING HIGHLY PURIFIED ALGINATES.
CN106243244B (en) A kind of carboxyl chitosan namo fluorescence probe and preparation method thereof with aggregation-induced emission characteristic
CN105670612B (en) A kind of preparation method of the fluorescence nano copper cluster gel sensitive to pH
CN105784616B (en) The method for detecting cysteine or acetylcysteine based on bimetal nano cluster
Liu et al. Formation mechanism of high-viscosity gelatinous egg white among “Fenghuang Egg”: Phenomenon, structure, and substance composition
CN105510543A (en) Method for detecting genotoxic agent in water by utilization of Pseudorasbora parva
DE2363854A1 (en) SUBSTRATE FOR PROTEINASE DETERMINATION
Sun et al. Rheological properties of the gels of biological surfactant sodium deoxycholate/amino acids/halide salts systems
Kulapina et al. Potentiometric sensor arrays for the individual determination of penicillin class antibiotics using artificial neural networks
CN106076193B (en) Using ionic liquid as undenatured collagen base biological surfactant of reaction medium and preparation method thereof
Zhao et al. An environmentally friendly AIE probe for CMC determination

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180126