CN107628689B - Blue algae treatment method - Google Patents
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- CN107628689B CN107628689B CN201710919342.7A CN201710919342A CN107628689B CN 107628689 B CN107628689 B CN 107628689B CN 201710919342 A CN201710919342 A CN 201710919342A CN 107628689 B CN107628689 B CN 107628689B
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Abstract
The invention discloses a covering shading film/enzyme/microbial agent/light source composite technology for treating blue-green algae by combining a covering shading film on water surfaces of rivers, lakes and reservoirs with biological enzyme and microbial agent; aiming at the characteristics of photosynthesis required by the growth of blue-green algae, the invention adopts a shading technology of covering a shading film by partial opening, namely, at least two layers of shading films with gas water holes are covered on the water surface at intervals, and sunlight or artificial supplementary light sources are utilized to enrich and dispose the blue-green algae on the water surface which covers the partial opening of the shading film while the photosynthesis of the blue-green algae is blocked in a large area; meanwhile, aiming at the eutrophication environment required by the growth of blue algae, the microbial agent is adopted to decompose, absorb and convert the eutrophication substances so as to change the eutrophication state of the water body. Biological enzyme is utilized to destroy the protein of the blue algae living body so as to achieve the effects of killing and inhibiting the growth of the blue algae; the algae toxin after the blue algae death is cracked, decomposed and absorbed by using biological enzyme and microbial agent.
Description
Technical Field
The invention relates to the field of water environment, in particular to a blue algae treatment method based on illumination, which is used for blue algae treatment in rivers, lakes and reservoir water bodies by combining a covering shading film/biological enzyme/microbial agent/light source algae gathering/fishing or killing blue algae treatment composite technology.
Background
With the development of social economy and the increase of population, the problem of water environment pollution is becoming more serious, at present, rivers, lakes and reservoirs in China have blue algae to a certain extent, the gathering and the outbreak of the blue algae can influence the environmental safety to reduce the quality of water resources, harm aquaculture industry, cause secondary pollution of water bodies to change the structures of the communities of the rivers and the lakes, reduce the diversity of species, and in addition, the blue algae can generate algal toxins after dying to influence the safety of drinking water.
At present, the following three methods mainly exist for treating blue algae: 1) the chemical method comprises the following steps: killing or inhibiting blue algae propagation by using a chemical algicide; 2) the physical method comprises the following steps: engineering and physical methods such as sewage diversion, water changing, sediment dredging, manual salvage, high-voltage discharge, ultrasonic treatment and the like; 3) the biological method comprises the following steps: the quantity of blue algae is controlled by microorganisms, algae-eating fishes and zooplankton. Among the three methods, chemical method (such as algicide) algae removal is an effective way with remarkable effect and quick effect, but the method is a method for treating symptoms and root causes and must be used with caution, and the method has the following problems to be solved urgently in the using process: 1) after the chemical algicide is used for removing algae, the algal toxins remain in the water body; 2) Chemical algae killing has toxic and side effects, secondary pollution is caused, the influence on water organisms is great, and rivers after chemical agents are used are not beneficial to ecological restoration; 3) the chemical algicide can only control the algae in the water body in a short time; at present, China has limited the use of chemical methods in water body treatment such as rivers and the like.
The physical water changing method only addresses the symptoms and does not address the root causes; the sediment dredging can reduce a large amount of nutrient substances such as organic carbon, nitrogen, phosphorus and the like stored in rivers and lakes, increase the flow and the water storage capacity, and is an effective way and measure for reducing endogenous pollution. However, large-scale dredging can destroy the original biological population structure and ecological environment of rivers, lakes and reservoirs, weaken the self-cleaning function of the reservoirs and bring negative effects on ecological restoration; the manual fishing efficiency is low and the cost is high; the high-voltage discharge and ultrasonic treatment have certain limitations, are only suitable for removing algae in water plants on a small scale, and cannot solve the problems of eutrophication and algae pollution of rivers and lakes.
The single biological method has no secondary pollution to the treatment of the blue algae, can degrade pollutants and has a synergistic effect on ecological restoration, but the treatment efficiency and the treatment effect of the blue algae can be influenced after the blue algae are exploded in a large scale. Therefore, the method for treating the blue algae, which is efficient, convenient, safe and pollution-free, is provided, and becomes a technical problem to be solved urgently in the field.
Disclosure of Invention
Aiming at the defects of the existing blue algae treatment technology, the photosynthesis and phototaxis required by the growth of the blue algae, the eutrophication environment of water and bottom mud and the characteristic that the blue algae is rich in protein, the invention provides a blue algae treatment composite technology for covering a shading film, biological enzyme, microbial agent, light source algae gathering, fishing or killing in rivers, lakes and reservoirs, which is used for treating the blue algae in the water of the rivers, lakes and reservoirs, realizes the inhibition and control of the growth and the outbreak of the blue algae and realizes the high-efficiency and long-acting treatment of the blue algae.
The invention is realized by the following technical scheme:
a blue algae treatment method based on illumination comprises the following specific steps:
1) according to the characteristic that blue algae is enriched in rivers, lakes, bays and side banks along with water flow and wind direction, light shielding areas and shading film covering open areas are arranged at intervals on the bays and the side banks;
at least two layers of shading films are laid on the water surfaces of rivers, lakes and reservoir surfaces in the shading areas, and two ends of each shading film are fixed on two banks to block the photosynthesis of blue algae in a large area; the shading films are uniformly distributed with air holes which penetrate through the shading films, and the air holes of the upper and lower shading films are staggered with each other; the air holes of the shading film are staggered with each other, so that water evaporation and air circulation are facilitated, accumulated water leakage caused by rainfall and the like is facilitated, and the bearing pressure of the shading film caused by air flow and rainwater is reduced;
in specific application, the shading film is prefabricated into an assembled module, a flexible film and a hard film are divided according to requirements, such as acrylic fibers, polyester fibers, PVC or PLA and other renewable or degradable materials, and the flexible film and the hard film can be fixed on cables or pins at two banks of rivers and lakes;
arranging artificial light sources (such as light sources like LEDs) in the open area of the covering light shielding film, wherein the artificial illumination intensity is 3000 lux-11000 lux, the wavelength is 300-625 nm, when the natural illumination intensity is less than 3000lux, the artificial light sources are started, and the light sources are used for inducing blue-green algae to be enriched in the open area, so that the blue-green algae treatment efficiency is improved; specific illumination methods are described in literature: study on influence of illuminance on vertical transformation characteristics of blue-green algae in Zhang Haichun, early-aged snow, Lichujie [ J ]. environmental pollution and control, 2010, 32(5): 64-67;
2) adding biological enzyme and microbial agent into blue algae growth outbreak water area
The biological enzyme comprises one or more of protease, amylase and the like, and the use type and the use amount of the enzyme are determined according to analysis and detection of blue algae and water quality components in specific engineering.
According to the invention, biological enzyme is added according to the characteristic that blue-green algae is rich in protein, and the biological enzyme can rapidly diffuse to the surface of blue-green algae cells to destroy algal membranes and permeate into the cells to destroy functional protein groups of the cells, so that the synthesis of cell protein is inhibited, the normal metabolism of the cells is stopped, the growth of the blue-green algae is finally inhibited, and the purpose of blue-green algae treatment is achieved; the biological enzyme can crack and decompose algal toxins generated after the blue algae die, and can crack and decompose eutrophic bottom mud enriched by blue algae spores, so that the nutritional environment required by the growth and outbreak of the blue algae is reduced;
the microbial agent comprises one or more of cellulose degrading bacteria, yeast, actinomycetes, lactic acid bacteria, bacillus subtilis, nitrobacteria and denitrifying bacteria, the dosage can be determined according to specific water body environment, and can be determined according to conventional technologies in the field (for example, the following documents are referenced as Wuxia, Xiyue Bo. the application research of a direct bacterium feeding method in urban heavily polluted river treatment [ J ]. environmental engineering report, 2014, 8(8):3331 and 3336; alternatively, Song Yajing, Xiyu Bo, yellow minium, the research of the composite microbial agent and the enzyme preparation for treating blue-green algae and lakes [ J. hydroelectricity energy science, 2012, 6(7):2173 and 2177; or honor brightness, Xiyu Bo, Yudai D, and the like), 2010(2) 35-37; or, Nie autumn moon, Xie Yue wave, Zhuang Jing, etc. the blue algae treatment experiment [ J ] by high-efficiency microorganisms, the research and development of world science and technology, 2008, 30(4): 430-; or, Wangping, Wudafu, Licorin, etc. applied Effective Microbiota (EM) to treat eutrophic source water experimental studies [ J ] environmental science studies 2004, 17(3): 39-43).
The microbial agents have an inhibiting effect on the growth of blue-green algae, can be rapidly propagated in bottom mud and a water body when being thrown into a blue-green algae growth outbreak water area, and treat nitrogen, phosphorus and carbon in the water body and the bottom mud through degradation and the like, so that the pollution is controlled, nutrient-rich substances in the water body and the mud are degraded and absorbed, the living environment of the blue-green algae is changed, and the blue-green algae growth outbreak conditions are inhibited and reduced;
the microbial agent and the biological enzyme carry out cracking and decomposition on the killed cyanobacteria algae toxins, and carry out decomposition, degradation, absorption, conversion and the like on the eutrophic bottom mud through the microbial agent.
Preferably, in the illumination-based blue algae treatment method provided by the invention, the aperture of the gas-water hole is 1-10 cm, and the hole density is 50-1000/m2。
Preferably, in the blue algae treatment method based on illumination, the distance between the shading film and the water surface is set according to precipitation, airflow and the like, and the preferable distance is 10-50 cm.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention overcomes the defects of a single blue algae treatment technology and lays a foundation for water environment treatment and water ecological restoration.
2. The invention blocks the photosynthesis required by the growth and the outbreak of the blue algae by covering the shading film, and destroys the protein of the blue algae living body by inoculating biological enzyme in the water body and the bottom mud so as to achieve the effects of killing the blue algae and inhibiting the growth of the blue algae; by adding the microbial agent, the killed cyanobacteria algal toxins can be cracked and decomposed, and the eutrophic bottom mud can be decomposed, degraded, absorbed and converted through the microbial agent, so that the eutrophic environment required by the growth of the cyanobacteria is changed, and the complete treatment of the cyanobacteria outbreak is realized.
3. The blue algae can be extracted manually or mechanically after being enriched, and the blue algae can be further killed and decomposed by adding a microbial agent and biological enzyme, so that the treatment efficiency is improved.
4. The microbial agent and the biological enzyme in the invention are both environment-friendly, the shading film is made of recyclable or degradable environment-friendly materials, and the shading film is green, safe and free of secondary pollution, so that the blue-green algae can be treated efficiently and long-term, the consumption of financial resources, manpower and material resources is reduced, and the popularization and the application are easy.
Drawings
FIG. 1 is a flow chart of the abatement process of the embodiment.
FIG. 2 is a schematic diagram of an embodiment of a structure of a light shielding film;
in the figure: 1. an upper light-shielding film; 2. a lower light-shielding film; 3. artificial light source (LED), 4, fixing device; 5. slope protection of river bank; 6. a gas water hole; 7. a river base; 8. a riverbed.
Detailed Description
The idea of the invention is further illustrated by the following specific examples:
the species referred to in the examples are all species conventional in the art, wherein:
1) yeasts, actinomycetes, lactic acid bacteria, nitrifying bacteria and denitrifying bacteria are described in the following references:
wuxi, Xiyuebao, direct fungus feeding method in urban heavily polluted river treatment [ J ]. environmental engineering report, 2014, 8(8): 3331-.
Song Yajing, Xie Yue Bo, Huang Xiao Dan, origin microbial inoculum for repairing urban polluted river [ J ]. environmental engineering report, 2012, 6(7):2173-2177.
Van Rong Liang, Xie Yu Mei, Yudianto D, and the like, research on the treatment of lake blue algae by compound microbial agents and enzyme preparations [ J ] hydroelectric energy science 2010(2):35-37.
Neie autumn moon, Xie Yue wave, Zhuang Jing, etc. blue algae treatment with high efficiency microorganism experiment [ J ] world science and technology research and development, 2008, 30(4): 430-.
Wangping, Wudafu, Licorilin, et al, applied Effective Microbiota (EM) for eutrophication source water experimental research [ J ]. environmental science research, 2004, 17(3):39-43.
The "thank you" in the above references is the same person as the "thank you" of the present inventors;
2) other bacterial species and biological enzymes are commercially available.
Example 1
5-10 months, the test site is a river in Jiangsu, the test average river width is about 80-100 m, the average water depth is 3-4 m, and the water area treatment volume of the project is about 5 multiplied by 105m, and the chlorophyll a concentration in the water area before treatment is 0.0150 mg/L. (in the embodiment, the method for detecting the concentration of chlorophyll a is a spectrophotometry, which can be specifically referred to in the national environmental pollutants monitoring methods standard revision technical guideline (HJ 168-2010)).
The blue algae is subjected to in-situ enrichment treatment by using a composite technology of covering shading film, biological enzyme, microbial agent and light source for treating the blue algae by combining the covering shading film with the biological enzyme and the microbial agent, and the treatment process flow chart is shown in figure 1:
1. as shown in fig. 2, the river is divided into a light shielding region and a covering light shielding film opening region, and the area ratio of the two regions is 5: 1;
the light-shielding region means: installing two shading films, namely an upper shading film 1 and a lower shading film 2 on the surface of a water body in a bay, wherein a river base 7 and a river bed 8 are arranged below the shading films; the shading film is made of PVC material, and two ends of the shading film are fixed at the river bank protection slope 5 through fixing devices 4 (anchor cables); the shading film is provided with through and uniformly distributed gas-water holes 6, the diameter of each gas-water hole 6 is about 1cm, and the hole density is about 1000/m2The air holes 6 of the upper and lower layers of shading films are distributed in a staggered way, and the distance between the shading films and the water surface is 10 cm.
The covering light shielding film open region means: an artificial light source 3 (an LED adjustable lamp) is arranged in the area which is not covered by the shading film, the light source intensity is 3000 lux-11000 lux, the wavelength is 300-625 nm (the illumination intensity and the wavelength can be adjusted), and the artificial light source 3 is started when the illumination intensity is less than 3000 lux; the intensity and wavelength of the artificial light source are determined according to the literature 'study on the influence of illuminance on the vertical transformation characteristic of blue-green algae' (Zhang Haichun et al, environmental pollution and prevention, 2010, 32(5): 64-67).
2. After the water surface is covered with a shading film, microbial agents and biological enzymes are inoculated in the bottom sludge and the water body of the water area, the microbial agents which are put in the embodiment comprise cellulose degrading bacteria, saccharomycetes, actinomycetes, lactic acid bacteria, bacillus subtilis, nitrobacteria and denitrifying bacteria, the putting ratio of the number of the viable bacteria is 2:3:1:1:5:2:2, and the total number of the bacterial colonies is 2.8 multiplied by 108～3.0×109cfu/ml, the amount charged was 100 ppm.
The biological enzymes added in the example are protease and amylase with the mass ratio of 1:3, and the adding amount of the biological enzymes is 5 ppm. The adding mode is that the water is dissolved in clear water according to the mass ratio of 1:1 and then is uniformly sprayed in a water area to be treated.
The concentration of chlorophyll a in a light source induced enrichment area reaches a peak value of 0.10mg/L within 12-24 h from the beginning of an experiment, the concentration of chlorophyll a after treatment for one month is 0.0062mg/L, and the concentration of chlorophyll a after treatment for 45d is 0.0005mg/L (the allowable concentration of chlorophyll a is 0.0010mg/L, wherein the standard is derived from the documents of Wangming, Liu Xue, Zhang Jianhui, a lake eutrophication evaluation method and a classification standard [ J ]. Chinese environmental monitoring, 2002, 18(5): 47-49.).
The method for detecting the concentration of chlorophyll a in this embodiment is a spectrophotometric method, which can be found in the national environmental pollutants monitoring methods standard revision technical guideline (HJ 168-2010).
According to the national standard (GB/T20466-2006), the content of microcystin (MC for short) is measured by adopting a rapid microcystin detection kit, the MC content in the water body before treatment is 0.670 mug/L, and the MC in the water body after treatment by using the method is not measured.
The protease used in the embodiment is alkaline protease, the amylase is alpha-amylase, in the specific application process, other conventional commercially available proteases and amylases can be used, the purpose of the invention can be achieved, and the adding amount and proportion of the biological enzymes can be comprehensively determined according to product specifications, water environment and blue algae outbreak degree.
The microbial agent added in the specific implementation process can determine the added strains and the added amount according to the specific water condition and the number of blue-green algae, and the added microbial agent comprises one or more of cellulose degrading bacteria, saccharomycetes, actinomycetes, lactic acid bacteria, bacillus subtilis, nitrobacteria and denitrifying bacteria, so that the purpose of the invention can be realized.
Example 2
And the test field is a certain lake or bay of south Jing of Jiangsu for 5-10 months, the test range is about 80m multiplied by 80m, the average water depth is 5-8 m, and the chlorophyll a concentration of a water area before treatment is 0.0100 mg/L.
The blue algae is subjected to in-situ enrichment treatment by using a composite technology of covering shading film, biological enzyme, microbial agent and light source for treating the blue algae by combining the covering shading film with the biological enzyme and the microbial agent:
1) dividing the river into a shading area and a covering shading film opening area, wherein the area ratio of the two areas is 8:1 in sequence; the area of the treatment area is 6400 square meters.
Three layers of shading films are arranged in the shading area 20cm away from the surface of the water body, two ends of each shading film are fixed on two sides of a lake and a bay, each shading film is made of acrylic fiber, the pore diameter of each air pore on each shading film is 3cm, and the pore density is 800 pores/m2The air holes of the upper and lower layers of shading films are distributed in a staggered way.
And an LED adjustable lamp is arranged in an area (the area which is not covered by the light shielding film) which covers the opening area of the light shielding film, the light source intensity is 3000 lux-11000 lux, the wavelength is 300-625 nm (the illumination intensity and the wavelength can be adjusted), and the light source is started when the illumination intensity is less than 3000 lux.
2) After the water surface is covered with a shading film, microbial inoculum and biological enzyme are inoculated in the bottom sludge and the water body of the water area, the microbial inoculum comprises cellulose degrading bacteria, saccharomycetes, actinomycetes, lactic acid bacteria, nitrobacteria and denitrifying bacteria, the ratio of the number of the viable bacteria to the number of the nitrobacteria to the denitrifying bacteria is 2:4:1:5:2:2 in sequence, and the total number of the bacterial colonies is 2.8 multiplied by 108～3.0×109cfu/ml, the amount charged was 50 ppm.
The biological enzyme is alkaline protease and alpha-amylase with the mass ratio of 1:2, the adding amount of the biological enzyme is 3ppm, and the adding mode is the same as that of the example 1.
The concentration of chlorophyll a in a light source induced enrichment region reaches a peak value of 0.05mg/L within 12-24 hours after the experiment is started, the concentration of chlorophyll a after treatment for one month is 0.0050mg/L, and the concentration of chlorophyll a after treatment for 45 days is 0.0003 mg/L.
According to the national standard (GB/T20466-2006), the content of microcystin (MC for short) is measured by adopting a rapid microcystin detection kit, the MC content in the water body before treatment is 0.340 mug/L, and the MC in the water body after treatment by using the method is not measured.
Example 3
5-10 months, the test site is a river in Zhenju Zhenjiang river, the average river width of the test site is about 25-30 m, the average water depth is 5-6 m, and the water area treatment volume of the project is about 5.4 multiplied by 105m, and the chlorophyll a concentration in the water area before treatment is 0.0200 mg/L.
The blue algae is subjected to in-situ enrichment treatment by using a composite technology of covering shading film, biological enzyme, microbial agent and light source for treating the blue algae by combining the covering shading film with the biological enzyme and the microbial agent:
1) dividing the river into a shading area and a covering shading film opening area, wherein the area ratio of the two areas is 6:1 in sequence; the area of the treatment area is 3000 square meters.
Two layers of shading films are arranged in the shading area of the river bay, the shading films are made of terylene materials, the pore diameter of air holes on the shading films is 5cm, and the hole density is 500/m2The air and water holes of the upper and lower layers of shading films are staggeredThe distance between the shading film and the water surface is 50 cm.
And an LED adjustable lamp is arranged in an area (the area which is not covered by the light shielding film) which covers the opening area of the light shielding film, the light source intensity is 3000 lux-11000 lux, the wavelength is 300-625 nm (the illumination intensity and the wavelength can be adjusted), and the light source is started when the illumination intensity is less than 3000 lux.
2) After the water surface is covered with a shading film, microbial agents and biological enzymes are inoculated in the bottom sludge and the water body of the water area, the microorganisms comprise saccharomycetes, actinomycetes, lactic acid bacteria, bacillus subtilis, nitrobacteria and denitrifying bacteria, the ratio of the number of the viable bacteria to the number of the nitrobacteria to the denitrifying bacteria is 3:2:1:5:2:1 in sequence, and the total number of the bacterial colonies is 2.8 multiplied by 108～3.0×109cfu/ml, the amount charged was 150 ppm.
The mass ratio of the added biological enzyme is 2:1, 10ppm of alkaline protease and alpha-amylase, in the same manner as in example 1.
The concentration of chlorophyll a in a light source induced enrichment region reaches a peak value of 0.10mg/L within 12-24 hours after the experiment is started, the concentration of chlorophyll a after the treatment for 40 days is 0.0075mg/L, and the concentration of chlorophyll a after the treatment for 50 days is 0.0008 mg/L.
According to the national standard (GB/T20466-2006), the content of microcystin (MC for short) is measured by adopting a rapid microcystin detection kit, the MC content in the water body before treatment is 0.340 mug/L, and the MC in the water body after treatment by using the method is not measured.
Example 4
And 5-10 months, wherein the test site is a certain reservoir in Jiangsu, the test range is about 100m multiplied by 100m, the average water depth is 8-10 m, and the chlorophyll a concentration of the water area before treatment is 0.0350 mg/L.
The blue algae is subjected to in-situ enrichment treatment by using a composite technology of covering shading film, biological enzyme, microbial agent and light source for treating the blue algae by combining the covering shading film with the biological enzyme and the microbial agent:
1) dividing the river into a shading area and a covering shading film opening area, wherein the area ratio of the two areas is 9:1 in sequence; the area of the treatment area is 10000 square meters.
Four layers of shading films are arranged in the shading area, the shading films are made of terylene materials, the pore diameter of air holes on the shading films is 10cm, and the hole density is 50 pieces/m2The air holes of the upper and lower light-shielding films are distributed in a staggered manner, and the distance between the light-shielding films and the water surface is 45 cm.
And an LED adjustable lamp is arranged in an area (the area which is not covered by the light shielding film) which covers the opening area of the light shielding film, the light source intensity is 3000 lux-11000 lux, the wavelength is 300-625 nm (the illumination intensity and the wavelength can be adjusted), and the light source is started when the illumination intensity is less than 3000 lux.
2) After the water surface is covered with a shading film, microbial agents and biological enzymes are inoculated in the bottom mud and the water body of the water area, the microorganisms comprise actinomycetes, lactic acid bacteria, bacillus subtilis, nitrobacteria and denitrifying bacteria, the ratio of the number of the viable bacteria is 2:1:4:1:2, and the total number of the bacterial colonies is 2.8 multiplied by 108～3.0×109cfu/ml, the amount put in was 200 ppm.
The mass ratio of the bio-enzyme dosed in this example is 3:1, and 12ppm of a biological enzyme, in the same manner as in example 1.
The concentration of chlorophyll a in a light source induced enrichment area reaches a peak value of 0.15mg/L within 12-24 hours after the experiment is started, the concentration of chlorophyll a after treatment for 45 days is 0.0080mg/L, and the concentration of chlorophyll a after treatment for 90 days is 0.0009 mg/L.
According to the national standard (GB/T20466-2006), the content of microcystin (MC for short) is measured by adopting a rapid microcystin detection kit, the MC content in the water body before treatment is 0.897 mu g/L, and the MC in the water body after treatment by using the method is not measured.
Example 5
The test land is a certain river of Yangzhou of Jiangsu, the average river width of the test land is about 45-50 m, the average water depth is 5-6 m, and the water area treatment volume of the project is about 7.5 multiplied by 105m, and the chlorophyll a concentration in the water area before treatment is 0.0300 mg/L.
The blue algae is subjected to in-situ enrichment treatment by using a composite technology of covering shading film, biological enzyme, microbial agent and light source for treating the blue algae by combining the covering shading film with the biological enzyme and the microbial agent:
1) dividing the river into a shading area and a covering shading film opening area, wherein the area ratio of the two areas is 10:1 in sequence; the area of the treatment area is 2500 square meters.
Four layers of shading films are arranged in the shading area, the shading films are made of PLA materials, the pore diameter of the air holes on the shading films is 8cm, and the hole density is 200/m2The air holes of the upper and lower light-shielding films are distributed in a staggered manner, and the distance between the light-shielding films and the water surface is 35 cm.
And an LED adjustable lamp is arranged in an area (the area which is not covered by the light shielding film) which covers the opening area of the light shielding film, the light source intensity is 3000 lux-11000 lux, the wavelength is 300-625 nm (the illumination intensity and the wavelength can be adjusted), and the light source is started when the illumination intensity is less than 3000 lux.
2) After the water surface is covered with a shading film, microbial agents and biological enzymes are inoculated in the bottom sludge and the water body of the water area, the microorganisms comprise cellulose degrading bacteria, saccharomycetes, bacillus subtilis, nitrobacteria and denitrifying bacteria, the ratio of the number of the viable bacteria is 1:3:5:1:1 in sequence, and the total number of the bacterial colonies is 2.8 multiplied by 108～3.0×109cfu/ml, the amount put in is 180 ppm.
The mass ratio of the biological enzyme added in the embodiment is 3:2, the dosage of the biological enzyme is 10ppm, and the dosage mode is the same as that of the example 1.
The concentration of chlorophyll a in a light source induced enrichment region reaches a peak value of 0.18mg/L within 12-24 hours after the experiment is started, the concentration of chlorophyll a after treatment for 45 days is 0.0100mg/L, and the concentration of chlorophyll a after treatment for 90 days is 0.0008 mg/L.
According to the national standard (GB/T20466-2006), the content of microcystin (MC for short) is measured by adopting a rapid microcystin detection kit, the MC content in the water body before treatment is 0.450 mug/L, and the MC in the water body after treatment by using the method is not measured.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, it is possible to make several modifications without departing from the principle of the present invention, such as using microbial agents and biological enzymes commonly used in the art, and these modifications should be considered as the protection scope of the present invention.
Claims (3)
1. A method for treating blue algae is characterized by comprising the following specific steps:
1) dividing a target water area into a shading area and a covering shading film opening area;
at least two layers of shading films are laid on the surface of the water body in the shading area, the shading films are provided with through air water holes, and the air water holes of the upper and lower layers of shading films are staggered with each other;
installing an artificial light source in the open area of the shading film, and starting the artificial light source when the natural illumination intensity is less than 3000 lux;
2) putting a microbial agent and biological enzyme into the water body, and treating the blue algae in the water body;
the intensity of the artificial light source is 3000 lux-11000 lux, and the wavelength is 300-625 nm;
the pore diameter of the gas water hole is 1-10 cm, and the pore density is 50-1000/m2；
The distance between the shading film and the surface of the water body is 10-50 cm;
the shading film is made of acrylic fibers, terylene, PVC or PLA materials;
the microbial agent comprises one or more of cellulose degrading bacteria, yeast, actinomycetes, lactic acid bacteria, bacillus subtilis, nitrobacteria and denitrifying bacteria;
the biological enzyme comprises at least one of protease and amylase;
after the water surface is covered with a shading film, a microbial agent and biological enzyme are inoculated in the bottom sludge and the water body of the water area, the microbial agent comprises cellulose degrading bacteria, saccharomycetes, actinomycetes, lactic acid bacteria, bacillus subtilis, nitrobacteria and denitrifying bacteria, the adding proportion of the number of the viable bacteria is 2:3:1:1:5:2:2, and the total number of the bacterial colonies is 2.8 multiplied by 108～3.0×109cfu/ml, the input amount is 100 ppm;
after the water surface is covered with a shading film, microbial inoculum and biological enzyme are inoculated in the bottom sludge and the water body of the water area, the microbial inoculum comprises cellulose degrading bacteria, saccharomycetes, actinomycetes, lactic acid bacteria, nitrobacteria and denitrifying bacteria, the ratio of the number of the viable bacteria is 2:4:1:5:2:2 in sequence, and the total number of the bacterial colonies is 2.8 multiplied by 108～3.0×109cfu/ml, the input amount is 50 ppm;
after the water surface is covered with shading film, the bottom mud and water body of said water area are inoculated with microbial inoculum and biological enzyme, the microbe includes microzymeActinomycetes, lactic acid bacteria, bacillus subtilis, nitrobacteria and denitrifying bacteria, wherein the ratio of viable count is 3:2:1:5:2:1 in sequence, and the total number of bacterial colonies is 2.8 multiplied by 108～3.0×109cfu/ml, the input amount is 150 ppm;
after the water surface is covered with a shading film, microbial agents and biological enzymes are inoculated in the bottom mud and the water body of the water area, the microorganisms comprise actinomycetes, lactic acid bacteria, bacillus subtilis, nitrobacteria and denitrifying bacteria, the ratio of the number of the viable bacteria is 2:1:4:1:2 in sequence, and the total number of the bacterial colonies is 2.8 multiplied by 108～3.0×109cfu/ml, the input amount is 200 ppm;
after the water surface is covered with a shading film, microbial agents and biological enzymes are inoculated in the bottom sludge and the water body of the water area, the microorganisms comprise cellulose degrading bacteria, saccharomycetes, bacillus subtilis, nitrobacteria and denitrifying bacteria, the ratio of the number of the viable bacteria is 1:3:5:1:1 in sequence, and the total number of the bacterial colonies is 2.8 multiplied by 108～3.0×109cfu/ml, the input amount is 180 ppm;
the technology is a blue algae treatment compound technology combining covering shading films, biological enzymes, microbial agents, light source algae gathering, fishing or killing.
2. The method for treating the blue algae according to claim 1, wherein the artificial light source is an LED light source.
3. The method for treating the cyanobacteria according to claim 2, wherein the area ratio of the light shielding region to the area covering the open region of the light shielding film is 5:1 to 10: 1.
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