CN107624794A - A kind of microorganism Control Technology of rice sheath blight disease - Google Patents
A kind of microorganism Control Technology of rice sheath blight disease Download PDFInfo
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- CN107624794A CN107624794A CN201710873034.5A CN201710873034A CN107624794A CN 107624794 A CN107624794 A CN 107624794A CN 201710873034 A CN201710873034 A CN 201710873034A CN 107624794 A CN107624794 A CN 107624794A
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Abstract
A kind of microorganism Control Technology of rice sheath blight disease, is related to the technical field of biological control of rice pest, comprises the following steps：Prepare NA solid mediums, prepare LB liquid mediums, prepare seed liquor, prepare zymotic fluid, prepare fermentation broth extract, prepare mixed agent, rice colonizes.Preventing and treating of the extractive from fermentative mixture jinggangmeisu to Rhizoctonia solani Kuhn has synergy, prevention effect of the jinggangmeisu to rice sheath blight disease can be significantly increased, certain theoretical foundation is provided to the prevention effect of rice sheath blight disease to improve jinggangmeisu, and an effective way is provided to expand the application of antagonistic bacterium biological pesticide.Silicon processing is added to significantly reduce the rice sheath blight disease incidence of disease and disease index, the incidence of disease, which reduces amplitude, can reach more than 30%, and disease index then reduces by more than 40%.Silicon processing can dramatically increase the resistance of banded sclerotial blight and the content of Different Organs silicon.
Description
Technical field
The present invention relates to the technical field of biological control of rice pest, and in particular to a kind of microorganism of rice sheath blight disease
Control Technology.
Background technology
Rice sheath blight disease, it is the Common Diseases for influenceing China's paddy growth, generally rice sheath blight disease can cause water
Rice underproduction 10%-20%, it is withered to often lead to rice lodging when serious, and output reduction up to arrive 50%-60%, and China's agricultural is given birth to
Production causes huge economic loss.
However, the effective agent of preventing and treating rice sheath blight disease is relatively simple at present, mainly jinggangmeisu.Due to making for a long time
With Rhizoctonia solani Kuhn generates a certain degree of resistance to the action of a drug to jinggangmeisu, and single jinggangmeisu is to rice sheath blight disease
Preventive effect substantially weakens so that the number used every year gradually increases, and usage amount also dramatically increases.Therefore, in order to improve rice
The preventive effect of banded sclerotial blight, it is extremely urgent to research and develop efficient pharmaceutical preparations.Biological control is a kind of efficient, security control rice banded sclerotial blight
The method of disease.In recent years, domestic and international expert has carried out numerous studies to rice sheath blight disease Antagonistic Fungi, achieves larger achievement, so
And study and show that single biological control effect is unsatisfactory, usually show larger variation.
The content of the invention
In order to which the biological control for solving the problems, such as single in the prior art is present, the invention provides a kind of rice sheath blight disease
Microorganism Control Technology, there is synergistic effect using the mixed preventing and treating rice sheath blight disease of mixed agent.
The present invention uses following technical scheme：
A kind of microorganism Control Technology of rice sheath blight disease, comprises the following steps：
Step 1：NA solid mediums are prepared, using peptone 10.0g, powdered beef 3.0g, sodium chloride 5.0g, agar
15.0g, glucose 2.5g, add distilled water to dissolve, and are settled to 1L, and PH is adjusted to 7 ± 0.1,121 DEG C of sterilizing 20min after packing；
Step 2：LB liquid mediums are prepared, tryptone 10g, yeast extract are added in 950ml deionized waters
5g, NaCl10g, stirring adjust pH to 7.0 with 5mol/LNaOH, 1L are settled to deionized water, in 15psi until solute dissolving
Steam sterilizing 21min under high pressure；
Step 3：Antagonistic bacterium is activated into 24h on NA solid mediums in 37 DEG C, thalline, which is made, with sterilized water suspends
Liquid, 10mL thallus suspension liquids are taken to be inoculated into the 250mL conical flasks equipped with 100mLLB liquid mediums, in 28 DEG C of constant temperature bar
With 220r/min speed concussion and cultivate 48h under part, seed liquor is obtained；
Step 4：Seed liquor is equipped with the 1000mL conical flasks of 300mLLB liquid mediums with 10% inoculum concentration access,
Magnetic agitation, rotating speed 1000r/min, continuous culture 84h is placed in 28 DEG C of shaken cultivation case, obtains zymotic fluid；
Step 5：By zymotic fluid and ethyl acetate with mass ratio 1:2 fully mix extraction 48h, take extract in 40 DEG C of rotations
Turn in evaporimeter, with 110r/min rotating speed rotary evaporation to paste, dissolved with sterilized water, it is 1000mg/L to be made into mass concentration
Fermentation broth extract, be placed in 4 DEG C of refrigerators and save backup；
Step 6：Aseptically, by fermentation broth extract and mass concentration 500mg/L jinggangmeisu in mass ratio
4:1 is well mixed to obtain mixed agent；
Step 7：The H of rice paddy seed mass concentration 15%2O220min is sterilized, soak seed 48h, during which changes water with distilled water
2-3 times, bud show money or valuables one carries unintentionally after at 30 DEG C illumination cultivation 4-5d, then choose and grow consistent seedling replanting in field, given birth in seedling
Mixed agent is sprayed in long the 10th, 20,50 and 80 day to rice leaf, and carries out when seedling grows to the 20th day plus silicon processing, i.e.,
2.0mmolL is added in Hoagland nutrient solutions-1K2SO3Solution, and use 2molL-1HCl adjusts pH to 5.6, is sprayed on
Rice leaf.
Preferably, described antagonistic bacterium is antagonistic bacterium SU8.
Preferably, described antagonistic bacterium SU8 is pseudomonas aeruginosa.
The beneficial effects of the present invention are：
1) preventing and treating of the extractive from fermentative mixture jinggangmeisu to Rhizoctonia solani Kuhn has synergy, can significantly increase well
Ridge mycin provides certain to the prevention effect of rice sheath blight disease for raising jinggangmeisu to the prevention effect of rice sheath blight disease
Theoretical foundation, and provide an effective way to expand the application of antagonistic bacterium biological pesticide.
2) plus silicon processing can significantly reduce the rice sheath blight disease incidence of disease and disease index, and the incidence of disease, which reduces amplitude, to be reached
To more than 30%, disease index then reduces by more than 40%.Silicon processing can dramatically increase the resistance and Different Organs silicon of banded sclerotial blight
Content.
3) method of the invention can be with the growth and development dynamics of adjusting and controlling rice plant, comprising beneficial Xi Yin groups in plant
There is the function such as growth-promoting, disease-resistant, degeneration-resistant in growth.It is theoretical according to plant microecology, long-term between these bacteriums and host
The relation of mutual reciprocity and mutual benefit can be established in evolutionary process.From ingeniously the relation such as raw interaction considers that these bacteriums will be more favourable between biology
In colonizing for biology, Biocontrol Effect can be preferably played.
4) present invention is to develop the important means of green rice industry using biological control, and antagonistic bacterium is preventing and treating rice line
The important Biocontrol microorganism of rot.The compound with bioactivity is found from plant, directly being sent out turns into novel pesticide, is
An important channel of agricultural chemicals research and development at present.
Embodiment
Below by the description to embodiment, the present invention is further detailed explanation, to help people in the art
Member has more complete, accurate and deep understanding to inventive concept of the invention, technical scheme.
Embodiment 1
A kind of microorganism Control Technology of rice sheath blight disease, comprises the following steps：
Step 1：NA solid mediums are prepared, using peptone 10.0g, powdered beef 3.0g, sodium chloride 5.0g, agar
15.0g, glucose 2.5g, add distilled water to dissolve, and are settled to 1L, and PH is adjusted to 7 ± 0.1,121 DEG C of sterilizing 20min after packing；
Step 2：LB liquid mediums are prepared, tryptone 10g, yeast extract are added in 950ml deionized waters
5g, NaCl10g, stirring adjust pH to 7.0 with 5mol/LNaOH, 1L are settled to deionized water, in 15psi until solute dissolving
Steam sterilizing 21min under high pressure；
Step 3：Antagonistic bacterium is activated into 24h on NA solid mediums in 37 DEG C, thalline, which is made, with sterilized water suspends
Liquid, 10mL thallus suspension liquids are taken to be inoculated into the 250mL conical flasks equipped with 100mLLB liquid mediums, in 28 DEG C of constant temperature bar
With 220r/min speed concussion and cultivate 48h under part, seed liquor is obtained；
Step 4：Seed liquor is equipped with the 1000mL conical flasks of 300mLLB liquid mediums with 10% inoculum concentration access,
Magnetic agitation, rotating speed 1000r/min, continuous culture 84h is placed in 28 DEG C of shaken cultivation case, obtains zymotic fluid；
Step 5：By zymotic fluid and ethyl acetate with mass ratio 1:2 fully mix extraction 48h, take extract in 40 DEG C of rotations
Turn in evaporimeter, with 110r/min rotating speed rotary evaporation to paste, dissolved with sterilized water, it is 1000mg/L to be made into mass concentration
Fermentation broth extract, be placed in 4 DEG C of refrigerators and save backup；
Step 6：Aseptically, by fermentation broth extract and mass concentration 500mg/L jinggangmeisu in mass ratio
4:1 is well mixed to obtain mixed agent；
Step 7：The H of rice paddy seed mass concentration 15%2O220min is sterilized, soak seed 48h, during which changes water with distilled water
2-3 times, bud show money or valuables one carries unintentionally after at 30 DEG C illumination cultivation 4-5d, then choose and grow consistent seedling replanting in field, given birth in seedling
Mixed agent is sprayed in long the 10th, 20,50 and 80 day to rice leaf, and carries out when seedling grows to the 20th day plus silicon processing, i.e.,
2.0mmolL is added in Hoagland nutrient solutions-1K2SO3Solution, and use 2molL-1HCl adjusts pH to 5.6, is sprayed on
Rice leaf.
Described antagonistic bacterium is antagonistic bacterium SU8；Described antagonistic bacterium SU8 is pseudomonas aeruginosa.
Claims (3)
1. the microorganism Control Technology of a kind of rice sheath blight disease, it is characterised in that comprise the following steps：
Step 1：Prepare NA solid mediums, using peptone 10.0g, powdered beef 3.0g, sodium chloride 5.0g, agar 15.0g,
Glucose 2.5g, adds distilled water to dissolve, and is settled to 1L, and PH is adjusted to 7 ± 0.1,121 DEG C of sterilizing 20min after packing；
Step 2：Prepare LB liquid mediums, added in 950ml deionized waters tryptone 10g, yeast extract 5g,
NaCl10g, stirring adjust pH to 7.0 with 5mol/LNaOH, 1L are settled to deionized water, in 15psi high pressures until solute dissolving
Lower steam sterilizing 21min；
Step 3：Antagonistic bacterium is activated into 24h in 37 DEG C on NA solid mediums, thallus suspension liquid is made with sterilized water, takes
10mL thallus suspension liquids are inoculated into the 250mL conical flasks equipped with 100mLLB liquid mediums, under 28 DEG C of constant temperature with
220r/min speed concussion and cultivate 48h, obtains seed liquor；
Step 4：Seed liquor is equipped with the 1000mL conical flasks of 300mLLB liquid mediums with 10% inoculum concentration access, magnetic force
Stirring, rotating speed 1000r/min, continuous culture 84h is placed in 28 DEG C of shaken cultivation case, obtains zymotic fluid；
Step 5：By zymotic fluid and ethyl acetate with mass ratio 1:2 fully mix extraction 48h, take extract to be steamed in 40 DEG C of rotations
Send out in instrument, with 110r/min rotating speed rotary evaporation to paste, dissolved with sterilized water, be made into the hair that mass concentration is 1000mg/L
Zymotic fluid extract, it is placed in 4 DEG C of refrigerators and saves backup；
Step 6：Aseptically, by fermentation broth extract and mass concentration 500mg/L jinggangmeisu in mass ratio 4:1
It is well mixed to obtain mixed agent；
Step 7：The H of rice paddy seed mass concentration 15%2O220min is sterilized, soak seed 48h, during which changes water 2-3 with distilled water
It is secondary, bud show money or valuables one carries unintentionally after at 30 DEG C illumination cultivation 4-5d, then choose and grow consistent seedling replanting in field, in growth of seedling
Mixed agent is sprayed to rice leaf within 10th, 20,50 and 80 day, and carried out when seedling grows to the 20th day plus silicon processing, that is, exist
2.0mmolL is added in Hoagland nutrient solutions-1K2SO3Solution, and use 2molL-1HCl adjusts pH to 5.6, is sprayed on water
Rice blade face.
A kind of 2. microorganism Control Technology of rice sheath blight disease according to claim 1, it is characterised in that：Described antagonism
Bacterium is antagonistic bacterium SU8.
A kind of 3. microorganism Control Technology of rice sheath blight disease according to claim 1, it is characterised in that：Described antagonism
Bacterium SU8 is pseudomonas aeruginosa.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114868751A (en) * | 2022-05-25 | 2022-08-09 | 海南广陵高科实业有限公司 | Prevention and control method for rice sheath blight disease |
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| CN102657221A (en) * | 2012-05-17 | 2012-09-12 | 南京农业大学 | Hydrogen-rich liquid plant growth regulator, and preparation method and application thereof |
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2017
- 2017-09-25 CN CN201710873034.5A patent/CN107624794A/en not_active Withdrawn
Patent Citations (5)
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| CN102676416A (en) * | 2011-03-09 | 2012-09-19 | 华中农业大学 | Rice sheath blight biocontrol strain RS-BC, microbial agent, preparation method and application |
| CN102657221A (en) * | 2012-05-17 | 2012-09-12 | 南京农业大学 | Hydrogen-rich liquid plant growth regulator, and preparation method and application thereof |
| CN103396954A (en) * | 2013-07-22 | 2013-11-20 | 河海大学 | Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer |
| CN105557110A (en) * | 2015-12-14 | 2016-05-11 | 安徽省中日农业环保科技有限公司 | Hybrid rice pest and disease prevention planting technology |
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| 彭双强等: "拮抗细菌发酵提取物与井冈霉素混配对水稻纹枯病的毒力研究", 《湖南农业科学》 * |
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| CN114868751A (en) * | 2022-05-25 | 2022-08-09 | 海南广陵高科实业有限公司 | Prevention and control method for rice sheath blight disease |
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