CN107517788A - A kind of method for preventing and treating rice blast - Google Patents
A kind of method for preventing and treating rice blast Download PDFInfo
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- CN107517788A CN107517788A CN201710873053.8A CN201710873053A CN107517788A CN 107517788 A CN107517788 A CN 107517788A CN 201710873053 A CN201710873053 A CN 201710873053A CN 107517788 A CN107517788 A CN 107517788A
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- rice
- rice blast
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- single bacterium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- Pest Control & Pesticides (AREA)
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- Agronomy & Crop Science (AREA)
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Abstract
A kind of method for preventing and treating rice blast, is related to the technical field of biological control of rice pest, and preparation method of the invention includes：Prepare gradient dilution liquid, prepare NA solid mediums, bacillus separation, prepare PDA culture medium, prepare spore liquid, rice colonizes.The present invention separate bacillus can secrete antibacterial material to suppress growth of pathogenic bacteria, and plant defense system is induced to resist pathogen invasion, sprouting of the strains tested to Pyricularia oryzae conidiospore, the extension of germ tube and the formation of appresorium have significant inhibitory action, the strain activity material of the present invention has stronger inhibitory action to Pyricularia oryzae, more than 80% is reached to Pyricularia oryzae mycelial growth inhibition rate, silicon processing is added to significantly reduce the rice seedling rice blast incidence of disease and disease index, the incidence of disease, which reduces amplitude, can reach more than 30%, disease index then reduces by more than 40%.Silicon processing can dramatically increase the resistance of rice blast and the content of Different Organs silicon.
Description
Technical field
The present invention relates to the technical field of biological control of rice pest, and in particular to a kind of side for preventing and treating rice blast
Method.
Background technology
Rice is Chinese first generalized grain crop, and its yield accounts for 40% or so of Grain in China yield.And the major disease of rice 3
Including rice blast, bacterial leaf-blight and the sheath and culm blight of rice, wherein rice blast is one of disease that maximum is threatened in Rice Production, by fungi
Pears are embraced mycotic infection and caused, and can occur in the whole developmental stage of rice.Because the sick epidemic rate is fast, in suitable environmental condition
Under, it can cause to be very popular in the short time, massive losses are brought to Rice Production.At present, breeding for disease resistance is agriculturally mainly taken
Gradually rice blast is controlled with chemical agent.But because the genetic background of Pyricularia oryzae is complicated, easily variation, disease-resistant variety difficulty are kept up with
The speed of mutation of pathogenic bacteria biological strain, frequently result in the new rice varieties with disease resistance and lost after planting several years
Resistance.And chemical prevention, not only pollute the environment, reduce paddy quality, destroy ecology, the drug resistance for also resulting in germ constantly increases
Add so as to reduce preventive effect.Therefore, it is more and more concerned to control and solve rice blast to explore new method.Therefore rice blast is found
Effectively, hypotoxicity, the biological control approach not influenceed by pathogen resistance are most important for grain security.
The content of the invention
According to problems of the prior art, the invention provides a kind of method for preventing and treating rice blast, have low
Toxicity, do not influenceed by pathogen resistance, and to the inhibiting rate of Pyricularia oryzae it is high the advantages that.
The present invention uses following technical scheme：
A kind of method for preventing and treating rice blast, comprises the following steps：
Step 1：Healthy rice leaf is chosen, 5cm leaf section is cut into, is put into the triangle equipped with the ethanol of mass concentration 75%
Ethanol is outwelled in vibration in bottle after washing 2 times, is added gradient dilution after sterilized water immersion 30min, is obtained gradient dilution liquid；
Step 2：NA solid mediums are prepared, using peptone 10.0g, powdered beef 3.0g, sodium chloride 5.0g, agar
15.0g, glucose 2.5g, add distilled water to dissolve, and are settled to 1L, and PH is adjusted to 7 ± 0.1,121 DEG C of sterilizing 20min after packing；
Step 3：Draw gradient dilution liquid to be coated on NA solid mediums, after drying up, in 30 DEG C of constant incubator
Middle to be inverted culture 48h, picking single bacterium colony, gradient is rule and obtains single bacterium colony again on NA solid mediums, by the single bacterium of acquisition
It is standby to fall to be stored in NA solid mediums test tube slant；
Step 4：PDA culture medium is prepared, peeling potatoes are cleaned, 200g potatos is weighed and is cut into small pieces, add boiling
20-30 minutes are boiled, with eight layers of filtered through gauze, add 20g agar, continues heating stirring and mixes, after agar has dissolved, add Portugal
20 grams of grape sugar, stirs, and supplies moisture after slightly cooling down again to 1000 milliliters of constant volumes, 115 DEG C sterilize 20 minutes；
Step 5：Picking single bacterium colony on NA solid mediums test tube slant, streak inoculation are placed in 25 in PDA culture medium
After cultivating 6d under DEG C constant temperature illumination condition, with oese picking colony into sterilized water, spore liquid is made into；
Step 6：The H of rice paddy seed mass concentration 15%2O220min is sterilized, soak seed 48h, during which changes water with distilled water
2-3 times, bud show money or valuables one carries unintentionally after at 30 DEG C illumination cultivation 4-5d, then choose and grow consistent seedling replanting in field, given birth in seedling
Spore liquid is sprayed in long the 10th, 20,50 and 80 day to rice leaf, and carries out when seedling grows to the 20th day plus silicon processing, i.e.,
2.0mmolL is added in Hoagland nutrient solutions-1K2SO3Solution, and use 2molL-1HCl adjusts pH to 5.6, is sprayed on
Rice leaf.
Preferably, the gradient dilution described in step 1 dilutes 2 times altogether, every time 2 times of dilution.
Preferably, the spore liquid concentration described in step 5 is 106- 107CFU/mL。
Preferably, described single bacterium colony is bacillus.
The beneficial effects of the present invention are：
1) bacillus can secrete antibacterial material to suppress growth of pathogenic bacteria, and induce plant defense system to resist cause of disease
Bacterium is invaded, and sprouting, the extension of germ tube and the formation of appresorium of the strains tested to Pyricularia oryzae conidiospore have significant suppression
Make and use, strain activity material of the invention has stronger inhibitory action to Pyricularia oryzae, and Pyricularia oryzae mycelial growth is suppressed
Rate reaches more than 80%.
2) plus silicon processing can significantly reduce the rice seedling rice blast incidence of disease and disease index, and the incidence of disease reduces amplitude can
To reach more than 30%, disease index then reduces by more than 40%.Silicon processing can dramatically increase the resistance and Different Organs of rice blast
The content of silicon.
3) method of the invention can be with the growth and development dynamics of adjusting and controlling rice plant, comprising beneficial Xi Yin groups in plant
There is the function such as growth-promoting, disease-resistant, degeneration-resistant in growth.It is theoretical according to plant microecology, long-term between these bacteriums and host
The relation of mutual reciprocity and mutual benefit can be established in evolutionary process.From ingeniously the relation such as raw interaction considers that these bacteriums will be more favourable between biology
In colonizing for biology, Biocontrol Effect can be preferably played.
4) present invention is to develop the important means of green rice industry using biological control, and bacillus is preventing and treating rice blast
Important Biocontrol microorganism.The compound with bioactivity is found from plant, directly being sent out turns into novel pesticide, is current
One important channel of agricultural chemicals research and development.
Embodiment
Below by the description to embodiment, the present invention is further detailed explanation, to help people in the art
Member has more complete, accurate and deep understanding to inventive concept of the invention, technical scheme.
Embodiment 1
A kind of method for preventing and treating rice blast, comprises the following steps：
Step 1：Healthy rice leaf is chosen, 5cm leaf section is cut into, is put into the triangle equipped with the ethanol of mass concentration 75%
Ethanol is outwelled in vibration in bottle after washing 2 times, is added gradient dilution after sterilized water immersion 30min, is obtained gradient dilution liquid；
Step 2：NA solid mediums are prepared, using peptone 10.0g, powdered beef 3.0g, sodium chloride 5.0g, agar
15.0g, glucose 2.5g, add distilled water to dissolve, and are settled to 1L, and PH is adjusted to 7 ± 0.1,121 DEG C of sterilizing 20min after packing；
Step 3：Draw gradient dilution liquid to be coated on NA solid mediums, after drying up, in 30 DEG C of constant incubator
Middle to be inverted culture 48h, picking single bacterium colony, gradient is rule and obtains single bacterium colony again on NA solid mediums, by the single bacterium of acquisition
It is standby to fall to be stored in NA solid mediums test tube slant；
Step 4：PDA culture medium is prepared, peeling potatoes are cleaned, 200g potatos is weighed and is cut into small pieces, add boiling
20-30 minutes are boiled, with eight layers of filtered through gauze, add 20g agar, continues heating stirring and mixes, after agar has dissolved, add Portugal
20 grams of grape sugar, stirs, and supplies moisture after slightly cooling down again to 1000 milliliters of constant volumes, 115 DEG C sterilize 20 minutes；
Step 5：Picking single bacterium colony on NA solid mediums test tube slant, streak inoculation are placed in 25 in PDA culture medium
After cultivating 6d under DEG C constant temperature illumination condition, with oese picking colony into sterilized water, spore liquid is made into；
Step 6：The H of rice paddy seed mass concentration 15%2O220min is sterilized, soak seed 48h, during which changes water with distilled water
2-3 times, bud show money or valuables one carries unintentionally after at 30 DEG C illumination cultivation 4-5d, then choose and grow consistent seedling replanting in field, given birth in seedling
Spore liquid is sprayed in long the 10th, 20,50 and 80 day to rice leaf, and carries out when seedling grows to the 20th day plus silicon processing, i.e.,
2.0mmolL is added in Hoagland nutrient solutions-1K2SO3Solution, and use 2molL-1HCl adjusts pH to 5.6, is sprayed on
Rice leaf.
Gradient dilution described in step 1 dilutes 2 times altogether, every time 2 times of dilution.
Spore liquid concentration described in step 5 is 106- 107CFU/mL。
Described single bacterium colony is bacillus.
Claims (4)
- A kind of 1. method for preventing and treating rice blast, it is characterised in that comprise the following steps：Step 1：Healthy rice leaf is chosen, is cut into 5cm leaf section, is put into the triangular flask equipped with the ethanol of mass concentration 75% Ethanol is outwelled in vibration after washing 2 times, added gradient dilution after sterilized water immersion 30min, obtained gradient dilution liquid；Step 2：Prepare NA solid mediums, using peptone 10.0g, powdered beef 3.0g, sodium chloride 5.0g, agar 15.0g, Glucose 2.5g, adds distilled water to dissolve, and is settled to 1L, and PH is adjusted to 7 ± 0.1,121 DEG C of sterilizing 20min after packing；Step 3：Draw gradient dilution liquid to be coated on NA solid mediums, after drying up, fallen in 30 DEG C of constant incubator Culture 48h is put, picking single bacterium colony, gradient is rule and obtains single bacterium colony again on NA solid mediums, and the single bacterium colony of acquisition is protected It is standby to be stored in NA solid mediums test tube slant；Step 4：PDA culture medium is prepared, peeling potatoes are cleaned, 200g potatos is weighed and is cut into small pieces, add boiling to boil 20-30 minutes, with eight layers of filtered through gauze, 20g agar is added, continue heating stirring and mix, after agar has dissolved, add grape 20 grams of sugar, stirs, and supplies moisture after slightly cooling down again to 1000 milliliters of constant volumes, 115 DEG C sterilize 20 minutes；Step 5：Picking single bacterium colony on NA solid mediums test tube slant, streak inoculation is in PDA culture medium, being placed in 25 DEG C of perseverances After cultivating 6d under warm illumination condition, with oese picking colony into sterilized water, spore liquid is made into；Step 6：The H of rice paddy seed mass concentration 15%2O220min is sterilized, soak seed 48h, during which changes water 2-3 with distilled water It is secondary, bud show money or valuables one carries unintentionally after at 30 DEG C illumination cultivation 4-5d, then choose and grow consistent seedling replanting in field, in growth of seedling Spore liquid is sprayed to rice leaf within 10th, 20,50 and 80 day, and carried out when seedling grows to the 20th day plus silicon processing, that is, exist 2.0mmolL is added in Hoagland nutrient solutions-1K2SO3Solution, and use 2molL-1HCl adjusts pH to 5.6, is sprayed on water Rice blade face.
- A kind of 2. method for preventing and treating rice blast according to claim 1, it is characterised in that：Ladder described in step 1 Degree dilution dilutes 2 times altogether, every time 2 times of dilution.
- A kind of 3. method for preventing and treating rice blast according to claim 1, it is characterised in that：Spore described in step 5 Sub- liquid concentration is 106- 107CFU/mL。
- A kind of 4. method for preventing and treating rice blast according to claim 1, it is characterised in that：Described single bacterium colony is bud Born of the same parents bacillus.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710873053.8A CN107517788A (en) | 2017-09-25 | 2017-09-25 | A kind of method for preventing and treating rice blast |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710873053.8A CN107517788A (en) | 2017-09-25 | 2017-09-25 | A kind of method for preventing and treating rice blast |
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| CN107517788A true CN107517788A (en) | 2017-12-29 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108060088A (en) * | 2018-01-11 | 2018-05-22 | 江西省农业科学院植物保护研究所 | A kind of culture medium for being used for Pyricularia oryzae culture, producing spore and preparation method thereof |
| CN108157092A (en) * | 2017-12-30 | 2018-06-15 | 青岛袁策生物科技有限公司 | A kind of method of growth period duration of rice disease control |
| CN116349694A (en) * | 2023-01-18 | 2023-06-30 | 华南农业大学 | Modified nano silicon dioxide and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009072070A (en) * | 2007-09-18 | 2009-04-09 | National Institute Of Agrobiological Sciences | Method for infecting rice blast, or the like, and method for efficiently assaying resistance of rice to blast, or the like, therewith |
| CN106754622A (en) * | 2017-01-10 | 2017-05-31 | 广西大学 | A kind of rice blast pathogen conidiospore for efficiently preventing pollution prepares cultural method |
-
2017
- 2017-09-25 CN CN201710873053.8A patent/CN107517788A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009072070A (en) * | 2007-09-18 | 2009-04-09 | National Institute Of Agrobiological Sciences | Method for infecting rice blast, or the like, and method for efficiently assaying resistance of rice to blast, or the like, therewith |
| CN106754622A (en) * | 2017-01-10 | 2017-05-31 | 广西大学 | A kind of rice blast pathogen conidiospore for efficiently preventing pollution prepares cultural method |
Non-Patent Citations (3)
| Title |
|---|
| 万树青: "《生物农药及使用技术》", 31 January 2003 * |
| 中国农业科学院植物保护研究所主编: "《中国农作物病虫害 上》", 31 December 1979 * |
| 游修龄: "《浙江省粮食生产上新台阶对策》", 31 May 1990 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108157092A (en) * | 2017-12-30 | 2018-06-15 | 青岛袁策生物科技有限公司 | A kind of method of growth period duration of rice disease control |
| CN108060088A (en) * | 2018-01-11 | 2018-05-22 | 江西省农业科学院植物保护研究所 | A kind of culture medium for being used for Pyricularia oryzae culture, producing spore and preparation method thereof |
| CN116349694A (en) * | 2023-01-18 | 2023-06-30 | 华南农业大学 | Modified nano silicon dioxide and preparation method and application thereof |
| CN116349694B (en) * | 2023-01-18 | 2024-06-11 | 华南农业大学 | Modified nano silicon dioxide and preparation method and application thereof |
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