CN107619874A - The anti-wheat ear germinating molecular labeling of one high flux and its application in breeding - Google Patents
The anti-wheat ear germinating molecular labeling of one high flux and its application in breeding Download PDFInfo
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- CN107619874A CN107619874A CN201710794889.9A CN201710794889A CN107619874A CN 107619874 A CN107619874 A CN 107619874A CN 201710794889 A CN201710794889 A CN 201710794889A CN 107619874 A CN107619874 A CN 107619874A
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Abstract
The invention belongs to biology field, specifically provide the anti-Genes of Pre-harvest Sprouting molecular labeling of a high flux and its application in breeding, this is one quick, efficiently, high flux detects the KASP molecular labelings for the crucial polynucleotides polymorphism (SNP) that anti-Genes of Pre-harvest Sprouting TaPHS1 promoter regions cause Preharvest Sprouting Resistance among Chinese Wheat Cultivars to change, can be made a variation the distribution situation in Chinese Wheat Cultivars with the efficient detection key, screening has the Wheat Germplasm Resources of anti growing out gene and efficient application is in wheat anti growing out molecular mark, improve wheat anti growing out breeding efficiency, seed selection anti growing out new varieties.
Description
Technical field
The present invention relates to plant molecular marker technical field, anti-Genes of Pre-harvest Sprouting of high flux is specifically provided
Molecular labeling and its application technology in breeding.
Background technology
Wheat (Triticum aestivum L.) is one of most important Three major grain crops in the world, its sown area
Ranked first in the world with yield.In China, wheat is second largest cereal crops, is only second to rice, long-term cultivated area is 2400
More than ten thousand hectares, total output is more than 1.2 hundred million tons, accounts for the 20% of cereal crops yield.Therefore, Wheat Production is relation China
One key factor of grain security and the living standard of people.
Wheat ear germinating (Pre-harvest Sprouting, abbreviation PHS) meets with the moon after referring to wheat maturation before harvest
The unbroken or moist environment of rain, the phenomenon for causing wheat seed to sprout, germinate on the tassel not harvested.Spike sprouting occurs
Afterwards, it can not only make the yield reduction of wheat, and the deterioration of wheat quality can be caused, while influence the holding conditions and shadow of wheat
Ring the Seed practical value of Second Year.Even if some seed outward appearances are not germinateed, but the nutriment of its internal reservoir have begun to decompose,
Conversion, can also reduce yield and quality, cause very big economic loss.Wheat ear germinating is several in Australia, Europe, the U.S.
Can all occur every year, caused by lose it is relatively serious.In southwestern Winter Wheat Area, middle and lower reach of Yangtze River Winter Wheat Area and the east of China
Bei Chunmaiqu, wheat ear germinating occurrence frequency are also very high.Therefore, Spike sprouting is a kind of limit for having a strong impact on world wheat production
Sexual factor processed.
Spike sprouting be one by inherent cause and the complicated quantitative character of such environmental effects, wherein grain color, tassel
Germination Inhibitors, seed hibernation feature in morphological feature, cob and wheat husk etc. are the main of influence wheat ear germinating resistance
Inherent cause.Domestic and foreign scholars utilize different genetic analysis colonies at present, in 2B, 2D, 3A, 3B, 3D, 4A, 4B, 4D of wheat
With the QTL site that multiple control wheat seed dormancy have been oriented on 7D chromosomes.Forefathers, which study, to be found in wheat 3A chromosomes
The main effect QTL of a control wheat ear germinating be present and be named as Q.Phs.Pseru-3A in end.Utilize map-based cloning
Clone the major gene resistance TaPHS1 of the wheat ear germinating on the QTL.
The TaPHS1 genes of anti growing out wheat and sense Spike sprouting wheat are sequenced and sequence alignment, find 63 altogether
Single nucleotide polymorphism (SNP), wherein 31 come from promoter region, 32 come from gene internal.In TaPHS1 gene promoters
There are 2 high frequency SNP sites in area, there is 3 high frequency SNP sites in code area.Early stage carries out fringe hair to part American Wheat Cultivars
Bud is identified and genotype detection, and two SNP for finding TaPHS1 code areas are the basic originals for causing most of varietal resistance to reduce
Therefore one, and promoter region SNP the American Wheat Cultivars ear germinating resistance studied is influenceed it is smaller.We compile in research in the recent period
Code area and promoter region SNP have found when influence on the ear germinating resistance of Chinese Wheat Cultivars, in code area Liang Ge sites SNP
For anti growing out genotype when, a SNP of promoter region associates extremely notable with ear germinating resistance, and the SNP is resistance equipotential
Average germination percentage during variant sites is is average germination percentage when feeling Spike sprouting allelic variation site far below the SNP, through system
Meter analysis finds the average germination percentage difference of two groups of materials extremely significantly (P=0.000).Illustrate the SNP in Chinese Wheat Cultivars
In be to influence one of ear germinating resistance crucial variation, be adapted to assisted Selection is marked in breeding using SNP designs
High flux KASP is marked, and the crucial variation for causing ear germinating resistance to change to TaPHS1 promoters in Chinese Wheat Cultivars is examined
Survey, the germplasm materials with anti growing out genotype can be gone out with assisting sifting, and be marked in breeding population with the mark
Assisted Selection, seed selection anti growing out new varieties, the breeding efficiency of raising wheat anti growing out.
The content of the invention
For blank existing for prior art, there is the high flux of important value in breeding the invention provides one
Anti- wheat ear germinating molecular labeling, can quickly, efficiently, high flux detection TaPHS1 promoter regions cause Wheat in China Spike sprouting
Crucial SNP that resistance changes variation, available for detecting the distribution situations of the crucial SNP in Wheat in China germ plasm resource,
Can high frequency zone there are the Wheat Germplasm Resources of anti growing out gene and be applied to wheat anti growing out molecular marker assisted selection
Breeding, improve wheat anti growing out breeding efficiency.
The technical solution adopted in the present invention is：
One anti-wheat ear germinating KASP molecular labeling, can quickly, on efficient, high flux detection TaPHS1 promoter regions it lead
The crucial SNP for causing Wheat in China ear germinating resistance to change, KASP high flux SNP markers should be labeled as, including 2 forward directions are drawn
Thing, 1 reverse primer；Primer sequence is as follows：
TaPHS1-pro-KASPFAM_C-R：
The ＇ of 5 ＇ GCTCCATGCACGCATCAGCGATCGAC 3, its nucleotide sequence such as SEQ ID NO:Shown in 1；
TaPHS1-pro-KASP-HEX_T-S:
5 ＇ ATTCCATGCACGCATCAGCGATCGAT3 ＇, its nucleotide sequence such as SEQ ID NO:Shown in 2；
TaPHS1-pro-KASP-R:
The ＇ of 5 ＇ CCAACGACGACCTGTACAAA 3, its nucleotide sequence such as SEQ ID NO:Shown in 3.Obtaining above-mentioned draw
After thing, it is as follows that inventor further provides specific detection method：
1. extract Wheat volatiles DNA to be detected using CTAB methods；
2. using the mark of above-mentioned design synthesis and the genomic DNA of upper step extraction in Q-Cycler 96PCR instrument (Hain
Life-science UK ltd.UK) amplification of enterprising performing PCR, and in ABI Quant StudioTM12K Flex real-time fluorescences are determined
Measure in PCR system (Life Technologies Corporation USA) and carry out SNP detections；
The standard of judgement is determined with the color put with the position for being gathered in transverse axis or the longitudinal axis：Signal is red, is gathered in horizontal stroke
Compare near axle and with anti growing out and flock together, be determined as anti growing out genotype；Signal is blueness, with the longitudinal axis and sense fringe
Germination control flocks together, and is judged to feeling Spike sprouting genotype；
3. the above-mentioned distribution situation for obtaining TaPHS1 promoter region keys SNP in wheat of analysis.
Specific method is as follows：
Wheat volatiles DNA to be detected is extracted using CTAB methods：
(a) wheat young leaflet tablet is taken to be placed in 96 hole depth orifice plates, using liquid nitrogen frozen and in grinds on tissue pulverizer
End；(b) after adding 800 μ L CTAB in 96 hole depth orifice plates, 65 DEG C of water-bath 90min are placed in, 5-8 times is during which gently shaken and make it that DNA is abundant
Cracking；(c) 800 μ L chloroform isoamyl alcohol (volume ratios 24 are added:1) 10min is gently shaken；(d) after being centrifuged under the conditions of 3000g in 10min
600 μ L of supernatant liquid are taken to be placed in the hole depth orifice plate of cleaning 96 new in addition (paying attention to corresponding sequence number)；(e) plus 600 μ L isopropanols (in advance-
20 DEG C of freezings) and 60 μ L 3M sodium acetates (pH=5.2) gently shake and shake up, you can see that white DNA floccules produce, be placed in -20 DEG C
60min increases DNA output.(f) 3000g outwells supernatant after centrifuging 10min, and with 70% ethanol (precipitation is put into -20 DEG C in advance
Refrigerator freezing) cleaning 2-3 times after air-dry to alcohol-free taste；(g) a certain amount of ddH is added2O dissolving DNAs, it is placed in -20 DEG C of refrigerators and preserves
It is standby；
Primer dilutes to be mixed with chemical examination primer：
Three primers are diluted to after 100 μM according to volume ratio with ultra-pure water
TaPHS1-pro-KASP-FAM_C-R：TaPHS1-pro-KASP-HEX_T-S：
TaPHS1-pro-KASP-R：Ultra-pure water=6：6：15：23 ratio carries out mixed chemical examination primer, preserve to-
20 DEG C standby；
Used PCR amplification system and program are as follows：
6 μ L PCR systems are prepared
System, the μ L of template DNA 3 (20ng/ μ L or so), the μ L (LGC of 2 × Master mix 3 are prepared on ice according to following table
Group UK), the KASP chemical examination μ L of primer 0.0825 (synthesis of Shanghai Sheng Gong biotech firms).
PCR programs are as follows：
1. 94 DEG C of pre-degeneration 5min；
2. 94 DEG C of denaturation 20s；
3. 65 DEG C of annealing 30s (each circulation reduces by 0.8 DEG C), step 2-3 is circulated 10 times.
4. 94 DEG C of denaturation 20s；
5. 57 DEG C of 30s that anneal, step 4-5 are circulated 35 times.
6. 10 DEG C of preservations.
Above-mentioned detection tagging and testing method provided by the present invention is directed to Wheat in China germplasm and kind；
In summary, the invention provides cause China on a kind of quick, efficient, high flux detection TaPHS1 promoter regions
The molecular labeling for the crucial SNP that wheat ear germinating resistance changes and corresponding detection method, crucial SNP can be detected in
Distribution situation in state's wheat, contribute to Wheat Germplasm Resources of the screening with anti growing out gene and applied to the anti-fringe hair of wheat
In bud molecular mark, different Wheat Germplasm Resources are detected, the germ plasm resource for choosing resistant site is made
For breeding parent, and after hybridization generation assisted Selection is marked, improve wheat anti growing out breeding efficiency.
Brief description of the drawings
Fig. 1 are SNP testing result figures in embodiment, and wherein transverse axis red spots represent C (anti growing out) genotype, are indulged
Axle blueness round dot represents T (sense Spike sprouting) genotype；× representative does not add DNA holes, and ■ represents ddH2O blank controls；
Fig. 2 is Fig. 1 colored schematic diagram.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, it is right below in conjunction with drawings and Examples
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Wheat breed employed in embodiment is that the conventional variety of China can obtain from market or other open channels.
The Wheat volatiles DNA of embodiment 1 is extracted
1) DNA extracts the collection of blade
In wheat tri-leaf period, wheat young leaflet tablet is chosen, is put into by number in corresponding 96 hole depth orifice bores, it is low on ice chest
Temperature, which preserves, prevents DNA degradation；
2) CTAB methods extraction Wheat volatiles DNA is utilized；- 20 DEG C are placed in save backup：
Above-mentioned steps are specific as follows：
(a) wheat young leaflet tablet is taken to be placed in 96 hole depth orifice plates, using liquid nitrogen frozen and in grinds on tissue pulverizer
End；(b) after adding 800 μ L CTAB in 96 hole depth orifice plates, 65 DEG C of water-bath 90min are placed in, 5-8 times is during which gently shaken and make it that DNA is abundant
Cracking；(c) 800 μ L chloroform isoamyl alcohol (volume ratios 24 are added:1) 10min is gently shaken；(d) after being centrifuged under the conditions of 3000g in 10min
600 μ L of supernatant liquid are taken to be placed in the hole depth orifice plate of cleaning 96 new in addition (paying attention to corresponding sequence number)；(e) plus 600 μ L isopropanols (in advance-
20 DEG C of freezings) and 60 μ L 3M sodium acetates (pH=5.2) gently shake and shake up, you can see that white DNA floccules produce, be placed in -20 DEG C
60min increases DNA output.(f) 3000g outwells supernatant after centrifuging 10min, and with 70% ethanol (precipitation is put into -20 DEG C in advance
Refrigerator freezing) cleaning 2-3 times after air-dry to alcohol-free taste；(g) a certain amount of ddH is added2O dissolving DNAs, it is placed in -20 DEG C of refrigerators and preserves
It is standby.
The primer of embodiment 2 dilutes to be mixed with chemical examination primer：
Three primers are diluted to after 100 μM according to volume ratio with ultra-pure water
TaPHS1-pro-KASP-FAM_C-R：TaPHS1-pro-KASP-HEX_T-S：
TaPHS1-pro-KASP-R：Ultra-pure water=6：6：15：23 ratio carries out mixed chemical examination primer, preserve to-
20 DEG C standby.
Embodiment 3PCR amplification systems and program are as follows：
6 μ L PCR systems are prepared
System, the μ L of template DNA 3 (20ng/ μ L or so), the μ L (LGC of 2 × Master mix 3 are prepared on ice according to following table
Group UK), the KASP chemical examination μ L of primer 0.0825 (synthesis of Shanghai Sheng Gong biotech firms).
PCR programs are as follows：
1. 94 DEG C of pre-degeneration 5min；
2. 94 DEG C of denaturation 20s；
3. 65 DEG C of annealing 30s (each circulation reduces by 0.8 DEG C), step 2-3 is circulated 10 times.
4. 94 DEG C of denaturation 20s；
5. 57 DEG C of 30s that anneal, step 4-5 are circulated 35 times.
6. 10 DEG C of preservations.
The interpretation of result of embodiment 4：
Utilize ABI Quant StudioTM12K Flex real-time fluorescence quantitative PCRs systems are entered to the PCR system expanded
The collection of row SNP distribution results, as shown in figure 1, wherein transverse axis is anti growing out genotype, the longitudinal axis is sense Spike sprouting genotype,
In 378 parts of wheat germplasms and kind on first 384 orifice plate, TaPHS1 promoter regions SNP genotype is C (anti-fringe hairs
Bud) have 11 parts, genotype is 365 parts of T (sense Spike sprouting) material, × number represent do not identify come 2 materials, ■ is institute
If ddH2O blank controls.
Embodiment 5
The 710 parts of wheat germplasms and kind of laboratory Collection and conservation are detected using molecular labeling provided by the invention
Afterwards, 21 parts of anti growing out Genotype (identical with anti growing out of the present invention control Rio Blanco genotype) is obtained, feels fringe
Germinate 686 parts of material (with the present invention sense Spike sprouting control NW97S186 genotype it is identical), 3 materials because of DNA cause for quality not
Detect genotype.Suitable germ plasm resource is chosen for wheat ear germinating point according to the result combination Spike sprouting phenotypic evaluation result
Sub- marker-assisted breeding is further studied.
Sequence table
<110>Shandong Agricultural University
<120>A kind of wheat ear germinating molecular labeling and its application in breeding
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 26
<212> DNA
<213>Artificial sequence ()
<400> 1
gctccatgca cgcatcagcg atcgac 26
<210> 2
<211> 26
<212> DNA
<213>Artificial sequence ()
<400> 2
attccatgca cgcatcagcg atcgat 26
<210> 3
<211> 20
<212> DNA
<213>Artificial sequence ()
<400> 3
ccaacgacga cctgtacaaa 20
Claims (2)
1. the anti-Genes of Pre-harvest Sprouting molecular labeling of a high flux, it is characterised in that：It should be marked labeled as KASP high fluxs SNP
Note, including 2 forward primers, 1 reverse primer；Primer sequence is as follows：
TaPHS1-pro-KASPFAM_C-R：
5 ＇ GCTCCATGCACGCATCAGCGATCGAC3 ＇, its nucleotide sequence such as SEQ ID NO:Shown in 1；
TaPHS1-pro-KASP-HEX_T-S:
5 ＇ ATTCCATGCACGCATCAGCGATCGAT3 ＇, its nucleotide sequence such as SEQ ID NO:Shown in 2；
TaPHS1-pro-KASP-R:
5 ＇ CCAACGACGACCTGTACAAA3 ＇, its nucleotide sequence such as SEQ ID NO:Shown in 3.
2. wheat ear germinating molecular labeling described in claim 1 is applied in wheat anti growing out molecular mark, carry
High wheat anti growing out breeding efficiency.
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| CN201710794889.9A CN107619874B (en) | 2017-09-06 | 2017-09-06 | High-throughput wheat head germination resistant molecular marker and application thereof in breeding |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109468406A (en) * | 2018-12-28 | 2019-03-15 | 中国农业科学院作物科学研究所 | KASP label relevant to Wheat Seedling root system configuration and its application |
| CN112538485A (en) * | 2019-09-23 | 2021-03-23 | 山东农业大学 | Novel functional allelic variation in wheat pre-harvest sprouting resistance gene TaPHS1 and SNP (single nucleotide polymorphism) marker, optimal haplotype and application thereof |
| CN113528700A (en) * | 2021-07-22 | 2021-10-22 | 湖北省农业科学院粮食作物研究所 | KASP molecular marker kit for detecting wheat ear germination resistance, detection method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011301A (en) * | 2016-08-03 | 2016-10-12 | 中国科学院成都生物研究所 | Primer for throughput identification of wheat seed major dormancy genes, and kit and method thereof |
| CN106048056A (en) * | 2016-08-05 | 2016-10-26 | 安徽农业大学 | Wheat seed dormancy persistence-substantially related SNP label and its CAPS label and use |
-
2017
- 2017-09-06 CN CN201710794889.9A patent/CN107619874B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011301A (en) * | 2016-08-03 | 2016-10-12 | 中国科学院成都生物研究所 | Primer for throughput identification of wheat seed major dormancy genes, and kit and method thereof |
| CN106048056A (en) * | 2016-08-05 | 2016-10-26 | 安徽农业大学 | Wheat seed dormancy persistence-substantially related SNP label and its CAPS label and use |
Non-Patent Citations (2)
| Title |
|---|
| SHUBING LIU等: "Independent mis-splicing mutations in TaPHS1 causing loss of preharvest sprouting (PHS) resistance during wheat domestication", 《NEW PHYTOLOGIST》 * |
| 张兆萍: "小麦穗发芽抗性的综合鉴定及对穗发芽相关基因TaPHS1的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109468406A (en) * | 2018-12-28 | 2019-03-15 | 中国农业科学院作物科学研究所 | KASP label relevant to Wheat Seedling root system configuration and its application |
| CN109468406B (en) * | 2018-12-28 | 2021-12-07 | 中国农业科学院作物科学研究所 | KASP marker related to wheat seedling stage root system configuration and application thereof |
| CN112538485A (en) * | 2019-09-23 | 2021-03-23 | 山东农业大学 | Novel functional allelic variation in wheat pre-harvest sprouting resistance gene TaPHS1 and SNP (single nucleotide polymorphism) marker, optimal haplotype and application thereof |
| CN112538485B (en) * | 2019-09-23 | 2022-09-20 | 山东农业大学 | Novel functional allelic variation in wheat pre-harvest sprouting resistance gene TaPHS1 and SNP (single nucleotide polymorphism) marker, optimal haplotype and application thereof |
| CN113528700A (en) * | 2021-07-22 | 2021-10-22 | 湖北省农业科学院粮食作物研究所 | KASP molecular marker kit for detecting wheat ear germination resistance, detection method and application |
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