CN103146700B - Rice variety 9194 black streaked dwarf restricting locus and molecular marking method thereof - Google Patents
Rice variety 9194 black streaked dwarf restricting locus and molecular marking method thereof Download PDFInfo
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Abstract
The invention relates to a rice variety 9194 black streaked dwarf restricting locus and a molecular marking method thereof. Four rice variety 9194 black streaked dwarf restricting locus are obtained by carrying out genetic linkage analysis on the black streaked dwarf resistance level of the genotype of an F2 single plant obtained from hybridization of the black streaked dwarf restricting rice variety 9194 and susceptible variety suyunuo and the corresponding F2:3 family, wherein qRBSDV3 is positioned between the markers RM282 and RM14898, qRBSDV6 is positioned between the markers RM20069 and RM30, qRBSDV9 is positioned between the markers RM3912 and RM257, and qRBSDV11 is positioned between the markers RM26062 and RM536. The molecular markers of the black streaked dwarf restricting gene are used for detecting whether the resistant variety 9194 and the derived varieties contain the black streaked dwarf restricting locus, so that the resistance level of the black streaked dwarf can be predicated, and the selecting efficiency of the black streaked dwarf restricting rice is greatly improved.
Description
Technical field
The invention belongs to molecular genetics field, relate to rice varieties 9194 anti-black streak dwarf site and molecule marking method thereof.
Technical background
Paddy rice is the important food crop of China.Black streaked dwarf virus of rice is a kind of virus disease being caused by rice black-streaked dwarf virus (Rice black-streaked dwarf virus, RBSDV), mainly by small brown rice planthopper, with persistence, without ovum mode, is propagated.In recent years, along with variation, the winter climate of cropping system and planting type warms and the spread of susceptible variety, pass virus mediator small brown rice planthopper generating capacity rising, black streaked dwarf virus of rice in Jiangsu, Zhejiang, Jiangxi and Fujian occurs on a large scale, and rise to rapidly the upper topmost virus disease of production, bring very large threat to the safety in production of paddy rice.The classical symptom of black streaked dwarf virus of rice is that diseased plant stunts, and leaf look dark green is stiff, and blade back, leaf sheath and cane have early stage wax, the irregular projection of billet line that the later stage is chocolate, and the strain of seriously falling ill is not eared.Producing above and there is no specific pesticide for this type of disease at present, is mainly early stage by the control to small brown rice planthopper, alleviate its harm, but prevention effect is unsatisfactory, and contaminate environment, destroys the eubiosis.To prevent and treat the optimal method of virus disease be seed selection and utilize disease-resistant variety, and screening, excavate and innovating disease-resistant germplasm is prerequisite and the basis of carrying out anti-black streak dwarf breeding.
Summary of the invention
The object of the invention is the above-mentioned deficiency for prior art, rice varieties 9194 anti-black streak dwarf site and molecule marking method thereof are provided.
Object of the present invention can be achieved through the following technical solutions:
The anti-black streak dwarf of rice varieties 9194 site, comprise the qRBSDV3 between molecule marker RM282-RM14898, at the qRBSDV6 between molecule marker RM20069-RM30, at the qRBSDV9 between molecule marker RM3912-RM257 and the qRBSDV11 between molecule marker RM26062-RM536; Described molecule marker RM282 primer is SEQ ID NO.1/SEQ ID NO.2, and the amplified band while there is qRBSDV3 is 146bp band; Described molecule marker RM14898 primer is SEQ ID NO.3/SEQ ID NO.4, and the amplified band while there is qRBSDV3 is 176bp band; Described molecule marker RM20069 primer is SEQ ID NO.5/SEQ ID NO.6, and the amplified band while there is qRBSDV6 is 162bp band; Described molecule marker RM30 primer is SEQ ID NO.7/SEQ ID NO.8, and the amplified band while there is qRBSDV6 is 136bp band; Described molecule marker RM3912 primer is SEQ ID NO.9/SEQ ID NO.10, and the amplified band while there is qRBSDV9 is 191bp band; Described molecule marker RM257 primer is SEQ ID NO.11/SEQ ID NO.12, and the amplified band while there is qRBSDV9 is 155bp band; Described molecule marker RM26062 primer is SEQ ID NO.13/SEQ ID NO.14, and the amplified band while there is qRBSDV11 is 149bp band; Described molecule marker RM536 primer is SEQ ID NO.15/SEQ ID NO.16, and the amplified band while there is qRBSDV11 is 242bp band.
The molecule marking method of the anti-black streak dwarf of rice varieties 9194 site qRBSDV3: with molecule marker RM282 primer: SEQ ID NO.1/SEQ ID NO.2 or with molecule marker RM14898 primer: SEQ ID NO.3/SEQ ID NO.4, the anti-black streak dwarf kind of amplifying rice or breeding material DNA, if can amplify the amplified fragments of 146bp with molecule marker RM282 primer, or with molecule marker RM14898 primer, can amplify the amplified fragments of 176bp, indicate the existence of Rice Varieties for Resistance black streak dwarf site qRBSDV3.
The molecule marking method of the anti-black streak dwarf of rice varieties 9194 site qRBSDV6: use molecule marker RM20069 primer: SEQ ID NO.5/SEQ ID NO.6, or with molecule marker RM30 primer: the SEQ ID NO.7/SEQ ID anti-black streak dwarf kind of NO.8 amplifying rice or breeding material DNA, if can amplify the amplified fragments of 162bp with molecule marker RM20069 primer, or with molecule marker RM30 primer, can amplify the amplified fragments of 136bp, indicate the existence of Rice Varieties for Resistance black streak dwarf site qRBSDV6.
The molecule marking method of the anti-black streak dwarf of rice varieties 9194 site qRBSDV9: use molecule marker RM3912 primer: SEQ ID NO.9/SEQ ID NO.10, or with molecule marker RM257 primer: the SEQ ID NO.11/SEQ ID anti-black streak dwarf kind of NO.12 amplifying rice or breeding material DNA, if can amplify the amplified fragments of 191bp with molecule marker RM3912 primer, or with the amplified fragments that molecule marker RM257 primer can amplify 155bp, all indicate the existence of Rice Varieties for Resistance black streak dwarf site qRBSDV9.
The molecule marking method of the anti-black streak dwarf of rice varieties 9194 site qRBSDV11: use molecule marker RM26062 primer: SEQ ID NO.13/SEQ ID NO.14, or with molecule marker RM536 primer: the SEQ ID NO.15/SEQ ID anti-black streak dwarf kind of NO.16 amplifying rice or breeding material DNA, if can amplify the amplified fragments of 149bp with molecule marker RM26062 primer, or with molecule marker RM536 primer, can amplify the amplified fragments of 242bp, indicate the existence of Rice Varieties for Resistance black streak dwarf site qRBSDV11.
The method of the molecule marker in the anti-black streak dwarf of rice varieties 9194 site that screening is described, comprises following steps:
(1) take anti-black streak dwarf kind 9194 as maternal, sense black streak dwarf japonica rice variety Suyunuo is male parent, hybridization F
1, built the F that comprises 200 individual plants
2segregating population, each F
2individual plant obtains corresponding F by selfing
2:3family, carries out anti-black streak dwarf evaluation;
(2) by SDS method, extract parent, F
1and F
2the DNA of each strain of colony, adopts simple sequence repeat marker SSR to carry out polymorphism screening to two parents, and PCR carries out on PTC-200 amplification instrument, and amplified production carries out electrophoretic analysis on 8% polyacrylamide gel, has polymorphic primer at F between parent
2in colony, analyze, obtain colony's genotype data;
(3) according to chain exchange rule, utilize colony's genotype data to build the genetic map of paddy rice, software used is MAPMARKER/EXP3.0, with Kosambi function, recombination value is converted to genetic distance;
(4) adopt field to bring out in conjunction with indoor inoculation and identify the Resistance Identification that carries out black streak dwarf;
(5) utilize MAPMARKER/EXP3.0 software to carry out linkage analysis to the black streak dwarf resistance rank of colony's genotype of each molecule marker and corresponding family, and Kosambi function is converted to genetic distance.Utilize Windows QTL Cartographer V2.0 software composite interval mapping method, the 2cM of take is scanning as step-length in full genome range.The detection of QTL adopts 5% overall conspicuous level, and the remarkable threshold of corresponding LOD statistic is estimated by permutation test method, is total to duplicate sampling 1000 times.Additive effect and the contribution rate in each site have been estimated simultaneously;
(6) obtain four of the anti-black streak dwarf gene locuss of anti-black streak dwarf kind 9194, be respectively qRBSDV3, between mark RM282-RM14898; QRBSDV6, between mark RM20069-RM30; QRBSDV9, between mark RM3912-RM257; QRBSDV11, between mark RM26062-RM536.Molecule marker by anti-black streak dwarf key-gene detects in resistant variety 9194 and derived varieties (being) thereof whether contain this key-gene site, and measurable its black streak dwarf resistance level, improves the efficiency of selection of anti-black streak dwarf paddy rice greatly.
The application of the anti-black streak dwarf of rice varieties 9194 of the present invention site in rice breeding.
The anti-black streak dwarf of rice varieties 9194 of the present invention site is the application in the anti-black streak dwarf kind of rapid screening or strain preferably.
The application of the molecule marker primer in the anti-black streak dwarf of rice varieties 9194 of the present invention site in rice breeding.
The application of the molecule marker primer in the anti-black streak dwarf of rice varieties 9194 of the present invention site in the anti-black streak dwarf kind of rapid screening or strain.
Beneficial effect
In earlier stage, applicant to derive from 20 countries totally 960 parts of rice varieties carried out anti-black streak dwarf field and bring out with artificial inoculation and identify, screen 4 rice varieties that black streak dwarf sickness rate is lower, wherein rice varieties 9194, within continuous 3 years, annual three place field tests are all to black streak dwarf performance high resistance, further indoor qualification result also shows, this kind has higher black streak dwarf resistance.The excavation in this anti-source is location, clone and the breeding utilization of the water resistant rice black streak dwarf gene material that provides the foundation.The present invention utilizes 9194 to hybridize with susceptible variety Suyunuo the F obtaining
2colony carries out genetic linkage analysis, obtains four anti-black streak dwarf gene locuss, is respectively qRBSDV3, qRBSDV6, qRBSDV9 and qRBSDV11.By detecting in resistant variety 9194 and derived varieties (being) thereof whether contain anti-black streak dwarf gene locus with the chain molecule marker of above-mentioned four gene locuss, measurable its black streak dwarf resistance level, improves the efficiency of selection of anti-black streak dwarf paddy rice greatly.The molecule marking method of the anti-black streak dwarf major gene loci of rice varieties 9194 provided by the present invention, has the following advantages:
(1) by the present invention, with SSR mark, located first in the world the gene locus of the anti-black streak dwarf in rice varieties 9194.
(2) the resistant gene site locality specific of locating by molecule marker of the present invention, it is convenient to identify.By detecting the chain molecule marker of these and anti-black streak dwarf gene locus, can predict the black streak dwarf resistance of rice plant, genotype detection for rice varieties or strain, to judge whether this kind or strain have black streak dwarf resistance, and then rapid screening disease-resistant variety or strain are for rice breeding.Resistant gene site easy to detect fast, not affected by environment;
(3) assistant breeding select target is clear and definite, cost-saving.In traditional breeding way, first to collect parent and the Cultivar with anti-source and carry out a series of hybridization, and will carry out individual plant selection to the resistance of black streak dwarf.Black streaked dwarf virus of rice is carried out to phenotypic evaluation complexity, simultaneously affected by environment, because black streak dwarf can not pass poison through ovum, pass the complicated qualification processes such as poison through raising small brown rice planthopper, band poison and inoculation, very difficult, the result reliability of phenotypic evaluation is low.Therefore, anti-black streak dwarf breeding is not only time-consuming, and difficulty is large, and cost is high.By detecting anti-black streak dwarf gene locus, can just identify in seedling stage the individual plant of anti-black streak dwarf, eliminate other plant, not only save production cost but also greatly improve the efficiency of selection of anti-black streak dwarf paddy rice.
Accompanying drawing explanation
The postvaccinal resistance performance of Fig. 1 9194 and Suyunuo.
Fig. 2 9194 and Suyunuo F
2colony's anti-black streak dwarf number of times distribution plan.
The distribution of the anti-black-streaked dwarf ospc gene of Fig. 3 rice varieties 9194 on karyomit(e).
The electrophoretogram of the anti-black-streaked dwarf ospc gene of Fig. 4 compact linkage molecule mark.M:Mark; 1:9194; 2: Suyunuo; 3:F
1; 4-13: extremely anti-individual plant; 14-23: utmost point sense individual plant.
Embodiment
Materials and methods:
(1) 9194/ Suyunuo F
2colony builds and phenotypic evaluation
(1) take anti-black streak dwarf kind 9194 as maternal, sense black streak dwarf japonica rice variety Suyunuo is male parent, and hybridization, has built 9194/ Suyunuo F
2segregating population, each F
2individual plant obtains corresponding 9194/ Suyunuo F by selfing
2:3family.
(2) phenotypic evaluation
(2.1) evaluation is brought out in field
Material is planted respectively in Huang Chuan town, Donghai County, Ganyu County soil cities and towns and experimental plot, Dong Xin farm, Guanyun County, and selecting surrounding is that material to be identified is planted in the plot of wheatland, to guarantee to obtain enough worms source.Within 2010 and 2011, material all divides two to repeat sowing in May 10 (first 3 weeks of wheat harvest), and each repeats single kind and broadcasts 80, uniform broadcasting 1 row, the long 50cm of row, line-spacing 10cm.June 27 transplanted, and single seedling is planted, distance between rows and hills 15cm * 20cm, and each kind is planted 60 caves.Conventional rich water quality management, does not use any agricultural chemicals.Expert evidence in 2012 was sowed respectively at May 5, May 10 and 3 dates of seeding of bu in Mays 15, each kind is broadcast 150, transplanting time is respectively June 20, June 25 and June 30, and each kind is planted 120 caves, and other planting patternss are identical with 2010 and 2011.Black streak dwarf is brought out as follows: first by dish bat method investigation small brown rice planthopper population density, the enamel basin of 33cm * 45cm * 5cm is aimed to rice shoot base portion and pat plant, small brown rice planthopper is clapped to be fallen within dish, then rapidly the small brown rice planthopper that falls into dish is imported to the high 60cm of being about, diameter is about in the plastic tank of 35cm.In early June, in experimental plot, investigate, fixed 5 samplings of planting material field diagonal lines, clap 0.30m2 at every, record small brown rice planthopper quantity.At random 100 small brown rice planthoppers that come from the collection of experiment field are carried out the RT-PCR detection of rice black-streaked dwarf virus, determine the malicious rate of taking.
In rice tillering Sheng phase late July statistics sickness rate.Now disease plant shows obvious symptom: extremely significantly stunt, lobus cardiacus is broken through inferior lobe leaf sheath and go out, part plant show as from lower pulvinus mouth stretch out in the shape of a spiral, that the top of partial blade occurs revolving shape is curling.And that healthy rice strain performance is tillered is vigorous, vitality is strong, but jointing not yet, disease plant and healthy plant contrast are very obvious.In addition, disease plant now seldom has osculant symptom, and the morbidity percentage mean value of each 2 repetition in test point of usining is added up as the anti-black streak dwarf phenotypic number of kind.
(2.2) artificial inoculation black-streaked dwarf virus
By parent, disease-resistant and susceptible check variety presoaking and germinating, to sow 200 and show money or valuables one carries unintentionally chitting piece in the plastic box of 70.5cm * 50.5cm * 41.5cm, bottom connects plastics tubing water flowing, keeps the about 3cm of water layer.Thinning when rice shoot grows to 2.5-3.0 leaf, eliminates sick and weak seedling, retains the strong seedling of approximately 150 strain neat and consistent, and seal with gauze on each plastic box top.The small brown rice planthopper that access gathered from East Sea Huang Chuan (test point, small brown rice planthopper is the highest with malicious rate) on June 10th, 2012 inoculates, and drives small brown rice planthopper every day for several times.After 7d, take away gauze, remove the small brown rice planthopper on seedling, postvaccinal transplantation of seedlings, to land for growing field crops, is grown to the Sheng of tillering and during the phase, investigated the state of an illness.Artificial inoculation each kind same period is done three repetitions, by 20 small brown rice planthoppers of every seedling, calculates and connects worm.
(2) 9194/ Suyunuo F
2the molecular marker analysis of colony
(1) by SDS method, extract parent, F
1and F
2the DNA of each strain of colony.
(2) ssr analysis is with reference to the program of Chen et al. (1997).10 μ l reaction systems comprise: 10mM Tris-HCl pH 8.3,50mM KCl, 1.5mM MgCl2,50 μ M dNTPs, 0.2 μ M primer, 0.5U Taq polymerase (TaKaRa, Dalian) and 20ng of DNA profiling.Amplified reaction carries out on PTC-200 (MJ Research Inc.) PCR instrument: 94 ℃ of 4min; 94 ℃ of 1min, 55 ℃ of 1min, 72 ℃ of 1.5min, 35 circulations; 72 ℃ of 7min.Amplified production is separated with 8% non-sex change PAGE glue, by silver, dyes colour developing, and silver dyes program and formulates and form according to the method for Sanguinetti et al. (1994).The lamp box that luminescent lamp is equipped with in the DNA band utilization of amplification is observed.Record result, between parent, have polymorphic primer at F
2in colony, analyze, obtain colony's genotype data;
(3) according to chain exchange rule, utilize colony's genotype data to build the genetic map of paddy rice, software used is MAPMAKER/EXP3.0, minimum LOD value is made as 2, obtains linkage map;
(4) utilize Windows QTL Cartographer V2.0 software (Wang et al., 2003) composite interval mapping method (Composite interval mapping, CIM), the 2cM of take is scanning as step-length in full genome range.The detection of QTL adopts 5% overall conspicuous level, and the permutation test for remarkable threshold of corresponding LOD statistic (Permutation test) (Churchill and Doerge, 1994) method is estimated, is total to duplicate sampling 1000 times.Additive effect and the contribution rate in each site have been estimated simultaneously.Utilize MAPMAKER/3.0 analysis software to carry out the compartment analysis of black streak dwarf resistance and SSR mark.And Kosambi function is converted to genetic distance (cM).
(3) results and analysis:
9194 and Suyunuo through the field of black streak dwarf and its average attack rate of indoor evaluation respectively 8.9% and 78%, show that 9194 have higher black streak dwarf resistance, and Suyunuo is to black streak dwarf extremely sensitive (Fig. 1).200 F
2:3family distributes and is continuous distribution the fastness frequency of black streak dwarf, shows may have a plurality of anti-black streak dwarf sites (Fig. 2) in this colony.From F
2in colony, select individual plant 10 strains that three places and indoor evaluation all show high resistance or high sense, carry out 12 chromosomal linkage analysises, in paddy rice the 3rd, 6,9 and 11 karyomit(e)s find there is chain sign with objective trait.Further built the 3rd, the linkage map of 6,9 and 11 whole chromosomes, in conjunction with phenotypic data, the QTL that utilizes Windows QTL Cartographer V2.0 software to carry out anti-black streak dwarf detects.Result detects an anti-black streak dwarf QTL qRBSDV3 between paddy rice the 3rd chromosomal marker RM282-RM14898, and LOD value is 2.34, and contribution rate is 5.4%; An anti-black streak dwarf QTL qRBSDV6 between paddy rice the 6th chromosomal marker RM20069-RM30, detected, LOD value is 4.42, and contribution rate is 10.3%; An anti-black streak dwarf QTL qRBSDV9 between paddy rice the 9th chromosomal marker RM3912-RM257, detected, LOD value is 6.63, and contribution rate is 35.5%; An anti-black streak dwarf QTL qRBSDV11 between paddy rice the 11st chromosomal marker RM26062-RM536, detected, LOD value is 3.55, and contribution rate is 31.1%.Above-mentioned four the soluble phenotypic variation 82.3%(of QTLs Fig. 3).
Molecule marker RM282 primer:
Upstream primer: CTGTGTCGAAAGGCTGCAC(SEQ ID NO.1)
Downstream primer: CAGTCCTGTGTTGCAGCAAG(SEQ ID NO.2)
Molecule marker RM14898 primer:
Upstream primer: ATGTTCAACCTTGTCCCGACTAACC(SEQ ID NO.3)
Downstream primer: TAAAGACGGCAGCTATCACTTTGC(SEQ ID NO.4)
With molecule marker RM282 primer: SEQ ID NO.1/SEQ ID NO.2 or with molecule marker RM14898 primer: SEQ ID NO.3/SEQ ID NO.4, the anti-black streak dwarf kind of amplifying rice or breeding material DNA, if can amplify the amplified fragments of 146bp with molecule marker RM282 primer, or with molecule marker RM14898 primer, can amplify the amplified fragments of 176bp, indicate exist (Fig. 4) of Rice Varieties for Resistance black streak dwarf site qRBSDV3.
Molecule marker RM20069 primer:
Upstream primer: GCGAGCGAGAGGAGAGATAGACG(SEQ ID NO.5)
Downstream primer: CGAATTCGGCACGAGTAATAGGG(SEQ ID NO.6)
Molecule marker RM30 primer:
Upstream primer: ACACTGTAGCGGCCACTG(SEQ ID NO.7)
Downstream primer: CCTCCACTGCTCCACATCTT(SEQ ID NO.8)
With molecule marker RM20069 primer: SEQ ID NO.5/SEQ ID NO.6, or with molecule marker RM30 primer: the SEQ ID NO.7/SEQ ID anti-black streak dwarf kind of NO.8 amplifying rice or breeding material DNA, if can amplify the amplified fragments of 162bp with molecule marker RM20069 primer, or with molecule marker RM30 primer, can amplify the amplified fragments of 136bp, indicate exist (Fig. 4) of Rice Varieties for Resistance black streak dwarf site qRBSDV6.
Molecule marker RM3912 primer:
Upstream primer: GCGAGCGAGAGGAGAGATAGACG(SEQ ID NO.9)
Downstream primer: CGAATTCGGCACGAGTAATAGGG(SEQ ID NO.10)
Molecule marker RM257 primer:
Upstream primer: ACACTGTAGCGGCCACTG(SEQ ID NO.11)
Downstream primer: CCTCCACTGCTCCACATCTT(SEQ ID NO.12)
With molecule marker RM3912 primer: SEQ ID NO.9/SEQ ID NO.10, or with molecule marker RM257 primer: the SEQ ID NO.11/SEQ ID anti-black streak dwarf kind of NO.12 amplifying rice or breeding material DNA, if can amplify the amplified fragments of 191bp with molecule marker RM3912 primer, or with the amplified fragments that molecule marker RM257 primer can amplify 155bp, all indicate exist (Fig. 4) of Rice Varieties for Resistance black streak dwarf site qRBSDV9.
Molecule marker RM26062 primer:
Upstream primer: TTTGCGTTTGCGTTTGCGTAGG(SEQ ID NO.13)
Downstream primer: ACCCGTACACCTCCACACAGTGC(SEQ ID NO.14)
Molecule marker RM536 primer:
Upstream primer: TCTCTCCTCTTGTTTGGCTC(SEQ ID NO.15)
Downstream primer: ACACACCAACACGACCACAC(SEQ ID NO.16)
With molecule marker RM26062 primer: SEQ ID NO.13/SEQ ID NO.14, or with molecule marker RM536 primer: the SEQ ID NO.15/SEQ ID anti-black streak dwarf kind of NO.16 amplifying rice or breeding material DNA, if can amplify the amplified fragments of 149bp with molecule marker RM26062 primer, or with molecule marker RM536 primer, can amplify the amplified fragments of 242bp, indicate exist (Fig. 4) of Rice Varieties for Resistance black streak dwarf site qRBSDV11.
By detecting in resistant variety 9194 and derived varieties (being) thereof whether contain anti-black streak dwarf gene locus with the chain molecule marker of above-mentioned four gene locuss, measurable its black streak dwarf resistance level, improves the efficiency of selection of anti-black streak dwarf paddy rice greatly.
Claims (3)
1. the anti-black streak dwarf of rice varieties 9194 site
qRBSDV6molecule marking method, it is characterized in that: use molecule marker RM20069 primer: SEQ ID NO.5/ SEQ ID NO.6, or with molecule marker RM30 primer: the anti-black streak dwarf kind of SEQ ID NO.7/ SEQ ID NO.8 amplifying rice DNA, if can amplify the amplified fragments of 162bp with molecule marker RM20069 primer, or with molecule marker RM30 primer, can amplify the amplified fragments of 136bp, indicate Rice Varieties for Resistance black streak dwarf site
qRBSDV6existence.
2. the anti-black streak dwarf of rice varieties 9194 site
qRBSDV6the application of molecule marker primer in rice breeding, the anti-black streak dwarf of wherein said rice varieties 9194 site
qRBSDV6molecule marker primer be selected from molecule marker RM20069 primer: SEQ ID NO.5/ SEQ ID NO.6, and/or molecule marker RM30 primer: SEQ ID NO.7/ SEQ ID NO.8.
3. application according to claim 2, is characterized in that the described anti-black streak dwarf of rice varieties 9194 site
qRBSDV6the application of molecule marker primer in the anti-black streak dwarf kind of rapid screening or strain.
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| CN103555719B (en) * | 2013-11-18 | 2015-08-19 | 江苏省农业科学院 | With the closely linked SSR marker of black streaked dwarf virus of rice Resistance QTL and application thereof on No. 11 karyomit(e)s |
| CN104046692A (en) * | 2014-06-25 | 2014-09-17 | 南京农业大学 | Rice black-streaked dwarf virus (RBSDV) resistant locus qRBSDV11 of rice variety 9194 and molecular marker method thereof |
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