CN107616955A - A kind of expelling parasite skin care item containing Japanese Stephania Root stem cell extract - Google Patents
A kind of expelling parasite skin care item containing Japanese Stephania Root stem cell extract Download PDFInfo
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Abstract
The invention discloses a kind of anthelmintic composition, the composition contains the composition of following parts by weight：1.5~9.4 parts of 0.07~2.1 part of Japanese Stephania Root stem cell extract, 0.8~3.9 part of cinnamomum camphora leaf extract, 1.1~6.5 parts of mint extract, 0.7~8.7 part of Herba Lysimachiae foenumgraeci extract and Ginger P.E.Meanwhile the invention also discloses the skin care item containing above-mentioned composition.The skin care item of the present invention contain Japanese Stephania Root stem cell extract and other several plant extracts, can effectively drive insect, especially mosquito, prevent insect-borne infection disease, while the skin care item of the present invention have good antipruritic effect；In addition, the skin care item of the present invention are free of preservative, and it is nontoxic to skin safe, the generation of skin allergic phenomena can be avoided.
Description
Technical field
The present invention relates to technical field of skin care, especially a kind of expelling parasite skin care containing Japanese Stephania Root stem cell extract
Product.
Background technology
Under the hygrothermal environment of south China, the insect such as mosquito, ant, cockroach is a lot, wherein, some serious diseases can lead to
Entomochory is crossed, such as malaria, Japanese Type-B encephalitis, dengue fever can pass through mosquitoes spread.However, the existing shield of in the market
Skin kind class is various, and most of skin care item, which have, nourishes skin, whitening or the effect of anti-aging；Existing skin care item it is big it is multi-functional compared with
It is single, or anthelminthic effect is general, therefore, after existing skin care item are smeared, it is also necessary to take other measures expelling parasite.
Japanese Stephania Root, alias Radix Stephaniae Cepharanthae, public mouse rattan, wild peach grass, firecracker disappear, towards day ointment, close cymbal grass, spun gold lotus
Leaf, day plaster, it is the root or cauline leaf of Menispermaceae stephania plant Japanese Stephania Root；With clearing heat and detoxicating, wind-expelling pain-stopping, inducing diuresis for removing edema
The effect of；It is usually used in abscess of throat, carbuncle swells boil, venomous snake bite, arthralgia pain due to rheumatism, stomachache, tinea pedis oedema；Its pharmacological action bag
Include：1) Muscle relaxation；2) ganglionic blocking action；3) foreign study proves that Japanese Stephania Root plays the role of to prevent and treat leukopenia.
On October 25th, 2010, Nature Biotechnology have delivered the research institute of the Unhwa Corp companies of South Korea
It is successfully separated first and has cultivated the paper of plant stem cell, and certification has been obtained to the identity of plant stem cell, plant does
The research of cell enters the stage of high speed development.
In simple terms, there are two " group " cells for being located at plant tip in plant, they have powerful self-renewing
Ability, it can not only be divided into autogenous cell, moreover it is possible to other any plant cells are divided into, are qualified " general-purpose cells ",
Moreover, they, which also have, continues splitting ability so that many large-scale plant existences more than thousand.
2012, " plant stem cell " technology was studied in the effect of beauty and health care field and has been achieved for certain progress,
Metabolism of the plant stem cell to raising skin is proved, improves microcirculation, tightening up and recovering skin elasticity has major application
Value.
The content of the invention
Based on this, providing one kind it is an object of the invention to overcome in place of above-mentioned the deficiencies in the prior art can effective expelling parasite
Skin care item, the skin care item are safe and non-toxic to skin, will not cause allergic.
To achieve the above object, this invention takes following technical scheme：
As the first aspect of the invention, the invention provides a kind of anthelmintic composition, the composition contains following
The composition of parts by weight：0.07~2.1 part of Japanese Stephania Root stem cell extract, 0.8~3.9 part of cinnamomum camphora leaf extract, mint extract
1.5~9.4 parts of 1.1~6.5 parts, 0.7~8.7 part of Herba Lysimachiae foenumgraeci extract and Ginger P.E.
Wherein, Japanese Stephania Root stem cell can play interference effect by epigenetic factor pair human skin cell, can have
The power of regeneration of effect excitation skin progenitor cell, hyperplasia fibrillar structure albumen, accelerate self more raw and reparation of skin；Yellow cinnamon leaf and essence
Oil can have analgesic, anti-suppressions, antibacterial, anti-spasm, cardiac stimulant, wind-dispelling, convergence, diuresis, excitement, sweating, drive is compacted etc. acts on, moreover it is possible to
Make vulneraia；Peppermint is one of Chinese conventional Chinese medicine, and it is pungent cool property sweating antipyretic medicine, control influenza, headache, hot eyes,
Disease, the external applications such as body heat, throat, gum swelling and pain can control neuralgia, pruitus, fash and eczema etc., usually with peppermint for tea, clearly
Heart improving eyesight；Lavender has the activity of resistance various bacteria, and linalool is the main component of Lavender antibacterial, its energy
Suppress the growth of 17 kinds of bacteriums (including gram positive bacteria and gram-negative bacteria), 10 kinds of fungies；Have confirmed Lavender and
Essential oil steam has certain antifungal activity, and 1% and 10% Herba Lysimachiae foenumgraeci extract can suppress the conidium of Botrytis cinerea
Sprout and gemma grows, and it is more effective to the inhibitory action comparison mycelia of gemma growth；Lavender steam can also suppress cigarette
Aspergillus Aspergillusfumigatus bacterium dead volume grows, and required dosage is suitable with tea oil；Scientific research discovery, ginger energy
Play a part of some antibiotic, it is especially more preferable to salmonella effect.Under the temperature of sweltering heat, food is easily by bacterium
Pollution, and growth and breeding is fast, easily causes acute gastroenteritis, and preventive and therapeutic effect can be played by eating a little gingers in right amount.Ginger extracts
Liquid has the effect of significantly inhibiting dermatophyte and killing connections Trichomonas vaginalis, can treat various carbuncle sore tumefacting virus.
Preferably, the mass ratio of the Japanese Stephania Root stem cell extract, cinnamomum camphora leaf extract and Ginger P.E is 0.12
~1.5：2：2.8~7.1.It is highly preferred that the matter of the Japanese Stephania Root stem cell extract, cinnamomum camphora leaf extract and Ginger P.E
Amount is than being 0.5：2：3.8.Present inventor has found through test of many times, when Japanese Stephania Root stem cell extract, perfume (or spice) in skin care item
The mass ratio of camphor tree leaf extract and Ginger P.E is 0.12~1.5：2：When 2.8~7.1, the anthelminthic effect of skin care item is preferable；
When the mass ratio of Japanese Stephania Root stem cell extract, cinnamomum camphora leaf extract and Ginger P.E in skin care item is 0.5：2：When 3.8, shield
Skin product expelling parasite it is better.
Preferably, the mass ratio of the Japanese Stephania Root stem cell extract and mint extract is 0.32~1.1：3.More preferably
Ground, the mass ratio of the Japanese Stephania Root stem cell extract and mint extract is 0.58：3.Present inventor is through repeatedly examination
Issue after examination and approval now, when the mass ratio of Japanese Stephania Root stem cell extract and mint extract is 0.32~1.1 in skin care item：When 3, skin care item
Suppression pruitus effect it is preferable；The mass ratio of Japanese Stephania Root stem cell extract and mint extract is in skin care item
0.58：When 3, the suppression pruitus of skin care item it is better.
Preferably, the composition contains the composition of following parts by weight：0.5 part of Japanese Stephania Root stem cell extract, yellow cinnamon leaf
3.8 parts of 2 parts of extract, 2.59 parts of mint extract, 7 parts of Herba Lysimachiae foenumgraeci extract and Ginger P.E.Present inventor passes through
Test of many times find, when in skin care item contain 0.5 part of Japanese Stephania Root stem cell extract, 2 parts of cinnamomum camphora leaf extract, mint extract
When 3.8 parts of 2.59 parts, 7 parts of Herba Lysimachiae foenumgraeci extract and Ginger P.E, the anthelminthic effect of skin care item and the effect for suppressing pruitus
Fruit is all good.
As the second aspect of the invention, present invention also offers a kind of expelling parasite skin care item, the skin care item include upper
State composition.
Preferably, Japanese Stephania Root stem cell extract, cinnamomum camphora leaf extract, mint extract, lavender in the skin care item
The quality percentage total amount of extract and Ginger P.E is 8.5~9.88%；Present inventor has found through test of many times, when
Japanese Stephania Root stem cell extract in skin care item, cinnamomum camphora leaf extract, mint extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E
When quality percentage total amount is 8.5~9.88%, the anthelminthic effect of skin care item is preferable.
Preferably, the Japanese Stephania Root stem cell extract is prepared by the following：
S1, Japanese Stephania Root new life root or branch sterilization, after removing xylem and marrow, it is inoculated in inducing culture, induction training
Support and obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain described Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.
Wherein, the micro-nano method broken wall of low temperature refers to make its freezing in a low temperature of Japanese Stephania Root stem cell is placed on into -170 DEG C, with
Melt at normal temperatures afterwards, so repeatedly so that the cell membrane of Japanese Stephania Root stem cell crushes；This method can live the life of stem cell
Property with power of regeneration assign skin care item in, absorbed via skin, can remarkably promote nutrition penetrate into and absorb, can effectively lift skin
Initiative, improve skin quality from cell aspect, strengthen the immunity of cell.
Preferably, the cinnamomum camphora leaf extract is prepared by the following：Yellow cinnamon leaf uses after high speed bead mill method broken wall
Water extraction obtains cinnamomum camphora leaf extract；
The mint extract is prepared by the following：Dried peppermint leaf is taken after ultrasonication, is obtained using alcohol extracting method thin
Lotus extract；
The Herba Lysimachiae foenumgraeci extract is prepared by the following：Lavender leaf is taken after the micro-nano method broken wall of low temperature, is used
Alcohol extracting method obtains Herba Lysimachiae foenumgraeci extract；
The Ginger P.E is prepared by the following：Ginger is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain Ginger P.E wherein, alcohol extracting method is preferably extracted using ethanol or methanol.
Relative to prior art, beneficial effects of the present invention are：
The skin care item of the present invention contain Japanese Stephania Root stem cell extract and other several plant extracts, can effectively drive elder brother
Worm, especially mosquito, insect-borne infection disease is prevented, while the skin care item of the present invention have good antipruritic effect；In addition, this hair
Bright skin care item are free of preservative, nontoxic to skin safe, can avoid the generation of skin allergic phenomena.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of embodiment of anthelmintic composition in the present invention, the composition contain the composition of following parts by weight：A thousand pieces of gold
0.07 part of rattan stem cell extract, 3.9 parts of cinnamomum camphora leaf extract, 1.1 parts of mint extract, 8.7 parts of Herba Lysimachiae foenumgraeci extract and life
1.5 parts of ginger extract.
A kind of embodiment of expelling parasite skin care item in the present invention, the skin care item include above-mentioned composition, Trivent OCG
96 parts of 34 parts, 19 parts of sorbierite, 42 parts of Arabic gum and water, wherein, it is Japanese Stephania Root stem cell extract, cinnamomum camphora leaf extract, thin
The percentage that the gross mass of lotus extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E accounts for skin care quality is 8.5%.
The specific preparation method of above-mentioned expelling parasite skin care item comprises the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature；
(2) cinnamomum camphora leaf extract is prepared by the following：Yellow cinnamon leaf is obtained after high speed bead mill method broken wall using water extraction
To cinnamomum camphora leaf extract；
Mint extract is prepared by the following：Dried peppermint leaf is taken after ultrasonication, is obtained using ethanol extraction method thin
Lotus extract；
Herba Lysimachiae foenumgraeci extract is prepared by the following：Lavender leaf is taken after the micro-nano method broken wall of low temperature, using methanol
Extraction method obtains Herba Lysimachiae foenumgraeci extract；
Ginger P.E is prepared by the following：Ginger is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
Ginger P.E；
(3) by cinnamomum camphora leaf extract, mint extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E and Trivent OCG 34
Part, 19 parts of sorbierite, 42 parts of Arabic gum and 96 parts of mixing of water, after 36 degrees Celsius stirs, are filtered to remove excessive moisture,
Lotion is obtained, then addition Japanese Stephania Root stem cell extract, which is uniformly dispersed, produces a kind of expelling parasite face cream.
Embodiment 2
A kind of embodiment of anthelmintic composition in the present invention, the composition contain the composition of following parts by weight：A thousand pieces of gold
0.23 part of rattan stem cell extract, 3.1 parts of cinnamomum camphora leaf extract, 3.7 parts of mint extract, 5.2 parts of Herba Lysimachiae foenumgraeci extract and life
5.7 parts of ginger extract.
A kind of embodiment of expelling parasite skin care item in the present invention, the skin care item include above-mentioned composition, Trivent OCG
88 parts of 41 parts, 26 parts of sorbierite, 55 parts of Arabic gum and water, wherein, it is Japanese Stephania Root stem cell extract, cinnamomum camphora leaf extract, thin
The percentage that the gross mass of lotus extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E accounts for skin care quality is 8.97%.
The specific preparation method of above-mentioned expelling parasite skin care item comprises the following steps：
(1) the Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life branch, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature；
(2) cinnamomum camphora leaf extract is prepared by the following：Yellow cinnamon leaf is obtained after high speed bead mill method broken wall using water extraction
To cinnamomum camphora leaf extract；
Mint extract is prepared by the following：Dried peppermint leaf is taken after ultrasonication, is obtained using methanol extraction thin
Lotus extract；
Herba Lysimachiae foenumgraeci extract is prepared by the following：Lavender leaf is taken after the micro-nano method broken wall of low temperature, using ethanol
Extraction method obtains Herba Lysimachiae foenumgraeci extract；
Ginger P.E is prepared by the following：Ginger is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
Ginger P.E；
(3) by cinnamomum camphora leaf extract, mint extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E and Trivent OCG 41
Part, 26 parts of sorbierite, 55 parts of Arabic gum and 88 parts of mixing of water, after 37 degrees Celsius stir, are filtered to remove excessive moisture,
Lotion is obtained, then addition Japanese Stephania Root stem cell extract, which is uniformly dispersed, produces a kind of expelling parasite face cream.
Embodiment 3
A kind of embodiment of anthelmintic composition in the present invention, the composition contain the composition of following parts by weight：A thousand pieces of gold
0.5 part of rattan stem cell extract, 2 parts of cinnamomum camphora leaf extract, 2.59 parts of mint extract, 7 parts of Herba Lysimachiae foenumgraeci extract and ginger carry
Take 3.8 parts of thing.
A kind of embodiment of expelling parasite skin care item in the present invention, the skin care item include above-mentioned composition, Trivent OCG
79 parts of 33 parts, 15 parts of sorbierite, 47 parts of Arabic gum and water, wherein, it is Japanese Stephania Root stem cell extract, cinnamomum camphora leaf extract, thin
The percentage that the gross mass of lotus extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E accounts for skin care quality is 9.88%.
The specific preparation method of above-mentioned expelling parasite skin care item comprises the following steps：
(1) the Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature；
(2) cinnamomum camphora leaf extract is prepared by the following：Yellow cinnamon leaf is obtained after high speed bead mill method broken wall using water extraction
To cinnamomum camphora leaf extract；
Mint extract is prepared by the following：Dried peppermint leaf is taken after ultrasonication, is obtained using ethanol extraction method thin
Lotus extract；
Herba Lysimachiae foenumgraeci extract is prepared by the following：Lavender leaf is taken after the micro-nano method broken wall of low temperature, using ethanol
Extraction method obtains Herba Lysimachiae foenumgraeci extract；
Ginger P.E is prepared by the following：Ginger is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
Ginger P.E；
(3) by cinnamomum camphora leaf extract, mint extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E and Trivent OCG 33
Part, 15 parts of sorbierite, 47 parts of Arabic gum and 79 parts of mixing of water, after 38 degrees Celsius stir, are filtered to remove excessive moisture,
Lotion is obtained, then addition Japanese Stephania Root stem cell extract, which is uniformly dispersed, produces a kind of expelling parasite face cream.
Embodiment 4
A kind of embodiment of anthelmintic composition in the present invention, the composition contain the composition of following parts by weight：A thousand pieces of gold
1.4 parts of rattan stem cell extract, 1.4 parts of cinnamomum camphora leaf extract, 5.4 parts of mint extract, 2.6 parts of Herba Lysimachiae foenumgraeci extract and ginger
7.1 parts of extract.
A kind of embodiment of expelling parasite skin care item in the present invention, the skin care item include above-mentioned composition, Trivent OCG
97 parts of 39 parts, 23 parts of sorbierite, 55 parts of Arabic gum and water, wherein, it is Japanese Stephania Root stem cell extract, cinnamomum camphora leaf extract, thin
The percentage that the gross mass of lotus extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E accounts for skin care quality is 8.7%.
The specific preparation method of above-mentioned expelling parasite skin care item comprises the following steps：
(1) the Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life branch, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature；
(2) cinnamomum camphora leaf extract is prepared by the following：Yellow cinnamon leaf is obtained after high speed bead mill method broken wall using water extraction
To cinnamomum camphora leaf extract；
Mint extract is prepared by the following：Dried peppermint leaf is taken after ultrasonication, is obtained using methanol extraction thin
Lotus extract；
Herba Lysimachiae foenumgraeci extract is prepared by the following：Lavender leaf is taken after the micro-nano method broken wall of low temperature, using methanol
Extraction method obtains Herba Lysimachiae foenumgraeci extract；
Ginger P.E is prepared by the following：Ginger is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
Ginger P.E；
(3) by cinnamomum camphora leaf extract, mint extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E and Trivent OCG 39
Part, 23 parts of sorbierite, 55 parts of Arabic gum and 97 parts of mixing of water, after 37.5 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition Japanese Stephania Root stem cell extract, which is uniformly dispersed, produces a kind of expelling parasite face cream.
Embodiment 5
A kind of embodiment of anthelmintic composition in the present invention, the composition contain the composition of following parts by weight：A thousand pieces of gold
2.1 parts of rattan stem cell extract, 0.8 part of cinnamomum camphora leaf extract, 6.5 parts of mint extract, 0.7 part of Herba Lysimachiae foenumgraeci extract and ginger
9.4 parts of extract.
A kind of embodiment of expelling parasite skin care item in the present invention, the skin care item include above-mentioned composition, Trivent OCG
89 parts of 37 parts, 21 parts of sorbierite, 43 parts of Arabic gum and water, wherein, it is Japanese Stephania Root stem cell extract, cinnamomum camphora leaf extract, thin
The percentage that the gross mass of lotus extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E accounts for skin care quality is 10.8%.
The specific preparation method of above-mentioned expelling parasite skin care item comprises the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature；
(2) cinnamomum camphora leaf extract is prepared by the following：Yellow cinnamon leaf is obtained after high speed bead mill method broken wall using water extraction
To cinnamomum camphora leaf extract；
Mint extract is prepared by the following：Dried peppermint leaf is taken after ultrasonication, is obtained using ethanol extraction method thin
Lotus extract；
Herba Lysimachiae foenumgraeci extract is prepared by the following：Lavender leaf is taken after the micro-nano method broken wall of low temperature, using ethanol
Extraction method obtains Herba Lysimachiae foenumgraeci extract；
Ginger P.E is prepared by the following：Ginger is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
Ginger P.E；
(3) by cinnamomum camphora leaf extract, mint extract, Herba Lysimachiae foenumgraeci extract and Ginger P.E and Trivent OCG 37
Part, 21 parts of sorbierite, 43 parts of Arabic gum and 89 parts of mixing of water, after 36.3 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition Japanese Stephania Root stem cell extract, which is uniformly dispersed, produces a kind of expelling parasite face cream.
The anthelminthic effect test of the skin care item of the present invention of embodiment 6
Test object：The face cream of embodiment 1~5 and the face cream 1~4 as control, wherein, the shield of control group 1~4
Skin frost differs only in Japanese Stephania Root stem cell extract, cinnamomum camphora leaf extract and Ginger P.E with the face cream of embodiment 3
Mass ratio it is different, its preparation method is same as Example 3；The Japanese Stephania Root stem cell extract of the face cream of control group 1~4,
The mass ratio of cinnamomum camphora leaf extract and Ginger P.E is followed successively by：0.12：2：2.8；0.8：2：5.2；1.1：2：6.4；1.5：2：
7.1；
Method of testing：Face cream is smeared on the back of the hand first, the rubber gloves of experiment is then put on, is cut on the back of the hand
5 square centimeters of region, expose the skin for scribbling face cream, hand is then stretched into mosquito cage (rest one in every 15 minutes in 3 hours
It is secondary), statistics falls on the quantity of mosquito on the skin for scribble face cream.
Test result：It is as shown in table 1 below；
The anthelminthic effect of face cream in the present invention of table 1
| Face cream | Mosquito populations | Face cream | Mosquito populations |
| Embodiment 1 | 12 | Control group 1 | 7 |
| Embodiment 2 | 9 | Control group 2 | 2 |
| Embodiment 3 | 0 | Control group 3 | 3 |
| Embodiment 4 | 10 | Control group 4 | 5 |
| Embodiment 5 | 15 |
Interpretation of result：As shown in Table 1, the face cream in the present invention can expelling parasite to a certain extent, wherein embodiment 3
The anthelminthic effect of the face cream of face cream and control group 1~4 is preferable, corresponding Japanese Stephania Root stem cell extract, yellow cinnamon leaf extraction
The mass ratio of thing and Ginger P.E all falls within 0.12~1.5：2：In the range of 2.8~7.1；Also, the face cream of embodiment 3
Anthelminthic effect it is best.
The antipruritic effect test of the skin care item of the present invention of embodiment 7
Test object：The face cream of embodiment 1~5 and the face cream 1~4 as control, wherein, the shield of control group 1~4
Skin frost is different from the mass ratio for differing only in Japanese Stephania Root stem cell extract and mint extract of the face cream of embodiment 3,
Its preparation method is same as Example 3；Japanese Stephania Root stem cell extract and mint extract in the face cream of control group 1~4
Mass ratio is 0.32 successively：3；0.74：3；0.96：3；1.1：3；
Method of testing：Nine kinds of face cream altogether, every group of face cream are chosen 15 25~35 years old adults and tested；Specifically
Method of testing is:First, the rubber gloves of experiment is put on, 6 square centimeters of region is then cut on the back of the hand, exposes the back of the hand
Skin, then hand is stretched into mosquito cage, face cream is smeared immediately after mosquito bite, counted and put down from smearing face cream to antipruritic
The equal time.
Test result：It is as shown in table 2 below；
The antipruritic effect of face cream in the present invention of table 2
| Face cream | The antipruritic time | Face cream | The antipruritic time |
| Embodiment 1 | 13.4 minutes | Control group 1 | 4.3 minute |
| Embodiment 2 | 10.7 minutes | Control group 2 | 2.6 minute |
| Embodiment 3 | 53 seconds | Control group 3 | 3.7 minute |
| Embodiment 4 | 3.1 minute | Control group 4 | 4.1 minute |
| Embodiment 5 | 3.82 minute |
Interpretation of result：As shown in upper table 2, the antipruritic effect of the face cream of embodiment 3~5 and control group 1~4 is preferable, right
The Japanese Stephania Root stem cell extract and the mass ratio of mint extract answered all fall within 0.32~1.1：In the range of 3；Moreover, with reality
The antipruritic ability for applying the face cream of example 3 is optimal, and the mass ratio of corresponding Japanese Stephania Root stem cell extract and mint extract is
0.58：3.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of anthelmintic composition, it is characterised in that the composition contains the composition of following parts by weight：Japanese Stephania Root stem cell carries
Take 0.07~2.1 part of thing, 0.8~3.9 part of cinnamomum camphora leaf extract, 1.1~6.5 parts of mint extract, Herba Lysimachiae foenumgraeci extract 0.7~
8.7 parts and 1.5~9.4 parts of Ginger P.E.
2. composition according to claim 1, it is characterised in that the Japanese Stephania Root stem cell extract, yellow cinnamon leaf extraction
The mass ratio of thing and Ginger P.E is 0.12~1.5：2：2.8~7.1.
3. composition according to claim 2, it is characterised in that the Japanese Stephania Root stem cell extract, yellow cinnamon leaf extraction
The mass ratio of thing and Ginger P.E is 0.5：2：3.8.
4. composition according to claim 1, it is characterised in that the Japanese Stephania Root stem cell extract and mint extract
Mass ratio be 0.32~1.1：3.
5. composition according to claim 4, it is characterised in that the Japanese Stephania Root stem cell extract and mint extract
Mass ratio be 0.58：3.
6. composition according to claim 1, it is characterised in that the composition contains the composition of following parts by weight：Thousand
Golden 0.5 part of rattan stem cell extract, 2 parts of cinnamomum camphora leaf extract, 2.59 parts of mint extract, 7 parts of Herba Lysimachiae foenumgraeci extract and ginger
3.8 parts of extract.
7. a kind of expelling parasite skin care item, it is characterised in that the skin care item include the combination as described in any one of claim 1~6
Thing.
8. skin care item according to claim 7, it is characterised in that Japanese Stephania Root stem cell extract, perfume (or spice) in the skin care item
Camphor tree leaf extract, mint extract, the quality percentage total amount of Herba Lysimachiae foenumgraeci extract and Ginger P.E are 8.5~9.88%.
9. skin care item according to claim 7, it is characterised in that the Japanese Stephania Root stem cell extract is by the following method
Obtain：
S1, Japanese Stephania Root new life root or branch sterilization, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain described Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.
10. skin care item according to claim 7, it is characterised in that the cinnamomum camphora leaf extract is prepared by the following：
Yellow cinnamon leaf obtains cinnamomum camphora leaf extract after high speed bead mill method broken wall using water extraction；
The mint extract is prepared by the following：Dried peppermint leaf is taken after ultrasonication, obtaining peppermint using alcohol extracting method carries
Take thing；
The Herba Lysimachiae foenumgraeci extract is prepared by the following：Lavender leaf is taken after the micro-nano method broken wall of low temperature, using alcohol extracting
Method obtains Herba Lysimachiae foenumgraeci extract；
The Ginger P.E is prepared by the following：Ginger is taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
Ginger P.E.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711062702.2A CN107616955A (en) | 2017-11-01 | 2017-11-01 | A kind of expelling parasite skin care item containing Japanese Stephania Root stem cell extract |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711062702.2A CN107616955A (en) | 2017-11-01 | 2017-11-01 | A kind of expelling parasite skin care item containing Japanese Stephania Root stem cell extract |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1352640A1 (en) * | 2000-12-15 | 2003-10-15 | Kabushiki Kaisha Yakult Honsha | Compositions for retarding skin aging |
| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
-
2017
- 2017-11-01 CN CN201711062702.2A patent/CN107616955A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1352640A1 (en) * | 2000-12-15 | 2003-10-15 | Kabushiki Kaisha Yakult Honsha | Compositions for retarding skin aging |
| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
Non-Patent Citations (1)
| Title |
|---|
| 王建新 等: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
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