CN107616952A - A kind of anti-wrinkle skin care compositions of the cell extract containing chrysanthemum and preparation method thereof - Google Patents
A kind of anti-wrinkle skin care compositions of the cell extract containing chrysanthemum and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of anti-wrinkle skin care item of cell extract containing chrysanthemum, the anti-wrinkle skin care item are made up of the component of following parts by weight：A phases：0.1~1.8 part of 0.5~2.0 part of chrysanthemum cell extract, 1.2~3.0 parts of apple extract, 0.6~2.5 part of malt extract and Rosmarinus officinalis extract；B phases：3~9 parts of 5~8 parts of mesoporous silicon and surfactant；C phases：40~60 parts of 3~5 parts of NMF and deionized water：D phases：0.5~2.0 part of 3~8 parts of hydrogenated coco and lanolin.There is good anti-oxidant anti-wrinkle effect in the anti-wrinkle skin care item of the present invention, skin metabolism can be promoted, skin microgroove can be reduced；Mesoporous silicon delays the release of active component, has the effect of sustained release and long-acting wrinkle-eliminating, avoids causing the waste of active component.
Description
Technical field
The present invention relates to skin care field, and in particular to a kind of anti-wrinkle skin care compositions of cell extract containing chrysanthemum and its
Preparation method.
Background technology
Skin care be it is important in global beauty and personal care industries segment market, and anti-aging skin care product is standby wherein
It is concerned.Since ancient times, the mankind are directed to finding the active material with anti-aging effects always.The reason for causing skin senescence
Mainly have：1) ultraviolet irradiation causes collagen to be lost in, so as to accelerate skin aging；2) attack of oxygen radical is led in vivo
Cause skin injury；3) the fibroblast natural aging in skin.
It is mainly crease-resistant to skin progress by adding hormone and plant extracts etc. in anti-aging skin care product on the market
Processing, but the abuse of hormone easily causes side effect, plant extracts Small side effects, but anti-aging short run effect unobvious,
Long-term use is needed, can not fundamentally reach the effect of anti-aging.Plant stem cell is undifferentiated cell in plant, is
The source to grow and signals-modulating center, there is very strong self-renewing and lasting splitting ability.Research shows, Mou Xiezhi
Thing stem cell has powerful anti-oxidant and activity of fighting against senium, can be mankind's anti-aging as the activator of human stem cell
Anti-wrinkle provides a new method.
The anti-ageing anti-wrinkle of skin is a long-term process, and in anti-ageing anti-wrinkle product on the market, there is burst effect,
Discharged completely in very short time or discharge most of active component, slow release effect is poor, does not possess lasting anti-aging anti-wrinkle
Effect, and prominent release of active component often leads to skin over-burden, on the other hand also results in the waste of active component.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of anti-wrinkle skin care of cell extract containing chrysanthemum
Composition.
It is another object of the present invention to provide a kind of preparation of the anti-wrinkle skin care compositions of cell extract containing chrysanthemum
Method.
To achieve the above object, the technical scheme that the present invention takes is as follows：
A kind of anti-wrinkle skin care item of cell extract containing chrysanthemum, are made up of the component of following parts by weight：
A phases：0.5~2.0 part of chrysanthemum cell extract, 1.2~3.0 parts of apple extract, malt extract 0.6~2.5
0.1~1.8 part of part and Rosmarinus officinalis extract；
B phases：3~9 parts of 5~8 parts of mesoporous silicon and surfactant；
C phases：40~60 parts of 3~5 parts of NMF and deionized water：
D phases：0.5~2.0 part of 3~8 parts of hydrogenated coco and lanolin.
In above-mentioned anti-wrinkle skin care item, chrysanthemum cell extract, apple extract, malt extract and Rosmarinus officinalis extract are
Anti-wrinkle active component, skin metabolism can be promoted, three is used cooperatively with good anti-ageing anti-wrinkle effect, can reduce flesh
Skin microgroove；Mesoporous silicon forms hydrogen bond by oxygen atom and the water-wet side of amphiphilic surfactant, and one is formed at oil-water interfaces
Layer protective layer, delays the release of active component, reaches the effect of sustained release and long-acting wrinkle-eliminating, avoids causing the waste of active component.
As the preferred embodiment of the anti-wrinkle skin care item of the cell extract of the present invention containing chrysanthemum, by following weight
The component of number is made：
A phases：1.0~1.5 parts of chrysanthemum cell extract, 1.8~2.4 parts of apple extract, malt extract 1.2~2.0
0.5~1.0 part of part and Rosmarinus officinalis extract；
B phases：3~9 parts of 5~8 parts of mesoporous silicon and surfactant；
C phases：40~60 parts of 3~5 parts of NMF and deionized water：
D phases：0.5~2.0 part of 3~8 parts of hydrogenated coco and lanolin.
As the preferred embodiment of the anti-wrinkle skin care item of the cell extract of the present invention containing chrysanthemum, by following weight
The component of number is made：
A phases：1.2 parts of chrysanthemum cell extract, 2.0 parts of apple extract, 1.5 parts of malt extract and Rosmarinus officinalis extract
0.8 part；
B phases：3~9 parts of 5~8 parts of mesoporous silicon and surfactant；
C phases：40~60 parts of 3~5 parts of NMF and deionized water：
D phases：0.5~2.0 part of 3~8 parts of hydrogenated coco and lanolin.
As the preferred embodiment of the anti-wrinkle skin care item of the cell extract of the present invention containing chrysanthemum, the NMF
For at least one of glycerine, glycerine, propane diols, 1,2- butanediols, amino acid, sodium lactate, allantoin, chondroitin sulfate.
As the preferred embodiment of the anti-wrinkle skin care item of the cell extract of the present invention containing chrysanthemum, the surface is lived
Property agent be Tween-80, lauric acid amide of ethanol, APG, monoolein, at least one in Emulsifier EL-60
Kind.
It is thin as the preferred embodiment of the anti-wrinkle skin care item of the cell extract of the present invention containing chrysanthemum, the chrysanthemum
Born of the same parents are chrysanthemum plant callus cell.
As the preferred embodiment of the anti-wrinkle skin care item of the cell extract of the present invention containing chrysanthemum, the mesoporous silicon
For hexagonal mesoporous silicon.
As the preferred embodiment of the anti-wrinkle skin care item of the cell extract of the present invention containing chrysanthemum, the mesoporous silicon
Particle diameter be 10~40nm.
Present invention also offers the preparation method of the anti-wrinkle skin care item of the above-mentioned cell extract containing chrysanthemum, including following step
Suddenly：
(1) synthesising mesoporous silicon；
(2) chrysanthemum cell extract is prepared；
(3) each component raw material is weighed in proportion；
(4) B phase components are thoroughly mixed, ultrasonic disperse forms blended emulsifier；
(5) A phases and C phases are thoroughly mixed to form aqueous phase in 45 DEG C of heating stirrings；
(6) D phases are heated with stirring to 70 DEG C~80 DEG C, are thoroughly mixed to form oil phase；
(7) blended emulsifier that step (4) obtains is mixed with the oil phase that step (6) obtains, is stirring evenly and then adding into water
Xiang Zhong, it is well mixed, obtains the anti-wrinkle skin care item.
As the preferred embodiment of the preparation method of the anti-wrinkle skin care item of the cell extract of the present invention containing chrysanthemum,
In the step (2), the chrysanthemum cell culture medium after culture chrysanthemum cell and the lysate of chrysanthemum cell are mixed, freezing is dry
It is dry, obtain freeze-dried powder.
As the preferred embodiment of the preparation method of the anti-wrinkle skin care item of the cell extract of the present invention containing chrysanthemum,
The freeze-dried powder crosses 200 mesh sieves.
Compared with prior art, the invention has the advantages that：
Chrysanthemum cell extract, apple extract, malt extract and rosemary extraction in the anti-wrinkle skin care item of the present invention
Thing all has good anti-oxidant anti-wrinkle effect, can promote skin metabolism, and three is used cooperatively with well anti-ageing
Anti-wrinkle effect, skin microgroove can be reduced；Mesoporous silicon forms hydrogen bond by oxygen atom and the water-wet side of amphiphilic surfactant,
Layer protective layer is formed at oil-water interfaces, delays chrysanthemum cell extract, apple extract, malt extract and rosemary extraction
The release of thing active component, reach the effect of sustained release and long-acting wrinkle-eliminating, avoid causing the waste of active component.
Embodiment
For the object, technical solutions and advantages of the present invention are better described, below in conjunction with specific embodiment to the present invention
Further illustrate.It will be appreciated by those skilled in the art that specific embodiment described herein is only to explain the present invention, not
For limiting the present invention.
Test method used in following embodiments is conventional method unless otherwise specified；Institute in following embodiments
The material that uses, reagent etc., unless otherwise specified, are commercially obtained.
Apple extract, malt extract and Rosmarinus officinalis extract are purchased from Xi'an You Shuo bio tech ltd.
Embodiment 1
A kind of embodiment of the preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum of the invention, described in the present embodiment
The preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum is：
(1) synthesising mesoporous silicon；
1) using lauryl amine as template, it is dissolved in ethanol solution, and adds tetraethyl orthosilicate under agitation, room temperature
Lower reaction 20h；
2) 1h is reacted respectively in 45 DEG C and 80 DEG C；
3) reaction product is placed in 1h under 90 DEG C of baking oven, obtains the hexagonal mesoporous silicon grain that size is 10~40nm.
(2) chrysanthemum cell extract is prepared
1) explant of chrysanthemum plant top children's spray section is taken, cleaning, sterilizing, is inoculated in callus inducing medium
On, induced under dark condition, temperature is that 25 DEG C of inductions produce callus cell；
Wherein, inducing culture is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), 6-benzyl aminopurine
(3.5mg/L), sucrose (20g/L) and agar (10g)；
2) by callus cell, continuous passage is bred in inducing culture, and screening cellular morphology is homogeneous, growth is stable
Chrysanthemum callus cell line；
3) step 2) is obtained into chrysanthemum callus cell line and is transferred to proliferated culture medium suspension culture, shaking table culture obtains steady
Fixed chrysanthemum suspension cell culture system；
Wherein, proliferated culture medium is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), kinetin (1.5mg/L),
Sucrose (20g/L)；Shaking speed 120rpm, 25 DEG C of temperature, is cultivated under illumination；
4) chrysanthemum suspension cell culture system is inoculated in proliferated culture medium, expands culture, obtain chrysanthemum cell；
5) proliferated culture medium after culture chrysanthemum cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes in culture medium
It is phenol red, retain culture medium in active component, and by cultivate chrysanthemum cell after proliferated culture medium and chrysanthemum cell lysate
Mixing, freeze-drying, obtain freeze-dried powder；
6) freeze-dried powder is crossed into 200 mesh sieves and obtains chrysanthemum cell extract.
(3) click part by weight and weigh each component raw material
A phases：1.2 parts of chrysanthemum cell extract, 2.0 parts of apple extract, 1.5 parts of malt extract and Rosmarinus officinalis extract
0.8 part；
B phases：2 parts of 6 parts of mesoporous silicon, 3 parts of Tween-80,1 part of APG and monoolein；
C phases：50 parts of 1 part of glycerine, 2 parts of glycerine, 0.5 part of amino acid and deionized water：
D phases：1.2 parts of 5 parts of hydrogenated coco and lanolin.
(4) B phase components are thoroughly mixed, ultrasonic disperse forms blended emulsifier；
(5) A phases and C phases are thoroughly mixed to form aqueous phase in 45 DEG C of heating stirrings；
(6) D phases are heated with stirring to 80 DEG C, are thoroughly mixed to form oil phase；
(7) blended emulsifier that step (4) obtains is mixed with the oil phase that step (6) obtains, is stirring evenly and then adding into water
Xiang Zhong, it is well mixed, obtains the anti-wrinkle skin care item.
Embodiment 2
A kind of embodiment of the preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum of the invention, described in the present embodiment
The preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum is：
(2) synthesising mesoporous silicon；
1) using lauryl amine as template, it is dissolved in ethanol solution, and adds tetraethyl orthosilicate under agitation, room temperature
Lower reaction 20h；
2) 1h is reacted respectively in 45 DEG C and 80 DEG C；
3) reaction product is placed in 1h under 90 DEG C of baking oven, obtains the hexagonal mesoporous silicon grain that size is 10~40nm.
(2) chrysanthemum cell extract is prepared
1) explant of chrysanthemum plant top children's spray section is taken, cleaning, sterilizing, is inoculated in callus inducing medium
On, induced under dark condition, temperature is that 25 DEG C of inductions produce callus cell；
Wherein, inducing culture is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), 6-benzyl aminopurine
(3.5mg/L), sucrose (20g/L) and agar (10g)；
2) by callus cell, continuous passage is bred in inducing culture, and screening cellular morphology is homogeneous, growth is stable
Chrysanthemum callus cell line；
3) step 2) is obtained into chrysanthemum callus cell line and is transferred to proliferated culture medium suspension culture, shaking table culture obtains steady
Fixed chrysanthemum suspension cell culture system；
Wherein, proliferated culture medium is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), kinetin (1.5mg/L),
Sucrose (20g/L)；Shaking speed 120rpm, 25 DEG C of temperature, is cultivated under illumination；
4) chrysanthemum suspension cell culture system is inoculated in proliferated culture medium, expands culture, obtain chrysanthemum cell；
5) proliferated culture medium after culture chrysanthemum cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes in culture medium
It is phenol red, retain culture medium in active component, and by cultivate chrysanthemum cell after proliferated culture medium and chrysanthemum cell lysate
Mixing, freeze-drying, obtain freeze-dried powder；
6) freeze-dried powder is crossed into 200 mesh sieves and obtains chrysanthemum cell extract.
(3) click part by weight and weigh each component raw material
A phases：1.2 parts of chrysanthemum cell extract, 2.0 parts of apple extract, 1.5 parts of malt extract and Rosmarinus officinalis extract
0.8 part；
B phases：8 parts of mesoporous silicon and 4 parts of lauric acid amide of ethanol, 3 parts of monoolein, 2 parts of Emulsifier EL-60；
C phases：40 parts of 2 parts of 1,2- butanediols, 0.5 part of sodium lactate, 0.5 part of allantoin and deionized water：
D phases：0.9 part of 6 parts of hydrogenated coco and lanolin.
(4) B phase components are thoroughly mixed, ultrasonic disperse forms blended emulsifier；
(5) A phases and C phases are thoroughly mixed to form aqueous phase in 45 DEG C of heating stirrings；
(6) D phases are heated with stirring to 80 DEG C, are thoroughly mixed to form oil phase；
(7) blended emulsifier that step (4) obtains is mixed with the oil phase that step (6) obtains, is stirring evenly and then adding into water
Xiang Zhong, it is well mixed, obtains the anti-wrinkle skin care item.
Embodiment 3
A kind of embodiment of the preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum of the invention, described in the present embodiment
The preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum is：
(3) synthesising mesoporous silicon；
1) using lauryl amine as template, it is dissolved in ethanol solution, and adds tetraethyl orthosilicate under agitation, room temperature
Lower reaction 20h；
2) 1h is reacted respectively in 45 DEG C and 80 DEG C；
3) reaction product is placed in 1h under 90 DEG C of baking oven, obtains the hexagonal mesoporous silicon grain that size is 10~40nm.
(2) chrysanthemum cell extract is prepared
1) explant of chrysanthemum plant top children's spray section is taken, cleaning, sterilizing, is inoculated in callus inducing medium
On, induced under dark condition, temperature is that 25 DEG C of inductions produce callus cell；
Wherein, inducing culture is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), 6-benzyl aminopurine
(3.5mg/L), sucrose (20g/L) and agar (10g)；
2) by callus cell, continuous passage is bred in inducing culture, and screening cellular morphology is homogeneous, growth is stable
Chrysanthemum callus cell line；
3) step 2) is obtained into chrysanthemum callus cell line and is transferred to proliferated culture medium suspension culture, shaking table culture obtains steady
Fixed chrysanthemum suspension cell culture system；
Wherein, proliferated culture medium is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), kinetin (1.5mg/L),
Sucrose (20g/L)；Shaking speed 120rpm, 25 DEG C of temperature, is cultivated under illumination；
4) chrysanthemum suspension cell culture system is inoculated in proliferated culture medium, expands culture, obtain chrysanthemum cell；
5) proliferated culture medium after culture chrysanthemum cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes in culture medium
It is phenol red, retain culture medium in active component, and by cultivate chrysanthemum cell after proliferated culture medium and chrysanthemum cell lysate
Mixing, freeze-drying, obtain freeze-dried powder；
6) freeze-dried powder is crossed into 200 mesh sieves and obtains chrysanthemum cell extract.
(3) click part by weight and weigh each component raw material
A phases：1.2 parts of chrysanthemum cell extract, 2.0 parts of apple extract, 1.5 parts of malt extract and Rosmarinus officinalis extract
0.8 part；
B phases：3 parts of 5 parts of mesoporous silicon and Tween-80；
C phases：55 parts of 2 parts of glycerine, 1 part of chondroitin sulfate and deionized water：
D phases：1.5 parts of 4 parts of hydrogenated coco and lanolin.
(4) B phase components are thoroughly mixed, ultrasonic disperse forms blended emulsifier；
(5) A phases and C phases are thoroughly mixed to form aqueous phase in 45 DEG C of heating stirrings；
(6) D phases are heated with stirring to 75 DEG C, are thoroughly mixed to form oil phase；
(7) blended emulsifier that step (4) obtains is mixed with the oil phase that step (6) obtains, is stirring evenly and then adding into water
Xiang Zhong, it is well mixed, obtains the anti-wrinkle skin care item.
Embodiment 4
A kind of embodiment of the preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum of the invention, described in the present embodiment
The preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum is：
(4) synthesising mesoporous silicon；
1) using lauryl amine as template, it is dissolved in ethanol solution, and adds tetraethyl orthosilicate under agitation, room temperature
Lower reaction 20h；
2) 1h is reacted respectively in 45 DEG C and 80 DEG C；
3) reaction product is placed in 1h under 90 DEG C of baking oven, obtains the hexagonal mesoporous silicon grain that size is 10~40nm.
(2) chrysanthemum cell extract is prepared
1) explant of chrysanthemum plant top children's spray section is taken, cleaning, sterilizing, is inoculated in callus inducing medium
On, induced under dark condition, temperature is that 25 DEG C of inductions produce callus cell；
Wherein, inducing culture is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), 6-benzyl aminopurine
(3.5mg/L), sucrose (20g/L) and agar (10g)；
2) by callus cell, continuous passage is bred in inducing culture, and screening cellular morphology is homogeneous, growth is stable
Chrysanthemum callus cell line；
3) step 2) is obtained into chrysanthemum callus cell line and is transferred to proliferated culture medium suspension culture, shaking table culture obtains steady
Fixed chrysanthemum suspension cell culture system；
Wherein, proliferated culture medium is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), kinetin (1.5mg/L),
Sucrose (20g/L)；Shaking speed 120rpm, 25 DEG C of temperature, is cultivated under illumination；
4) chrysanthemum suspension cell culture system is inoculated in proliferated culture medium, expands culture, obtain chrysanthemum cell；
5) proliferated culture medium after culture chrysanthemum cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes in culture medium
It is phenol red, retain culture medium in active component, and by cultivate chrysanthemum cell after proliferated culture medium and chrysanthemum cell lysate
Mixing, freeze-drying, obtain freeze-dried powder；
6) freeze-dried powder is crossed into 200 mesh sieves and obtains chrysanthemum cell extract.
(3) click part by weight and weigh each component raw material
A phases：1.2 parts of chrysanthemum cell extract, 2.0 parts of apple extract, 1.5 parts of malt extract and Rosmarinus officinalis extract
0.8 part；
B phases：4 parts of 5 parts of mesoporous silicon, 2 parts of Tween-80 and lauric acid amide of ethanol；
C phases：40 parts of 3 parts of propane diols, amino acid/11 part, 1 part of allantoin and deionized water：
D phases：0.5 part of 8 parts of hydrogenated coco and lanolin.
(4) B phase components are thoroughly mixed, ultrasonic disperse forms blended emulsifier；
(5) A phases and C phases are thoroughly mixed to form aqueous phase in 45 DEG C of heating stirrings；
(6) D phases are heated with stirring to 70 DEG C, are thoroughly mixed to form oil phase；
(7) blended emulsifier that step (4) obtains is mixed with the oil phase that step (6) obtains, is stirring evenly and then adding into water
Xiang Zhong, it is well mixed, obtains the anti-wrinkle skin care item.
Embodiment 5
A kind of embodiment of the preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum of the invention, described in the present embodiment
The preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum is：
(5) synthesising mesoporous silicon；
1) using lauryl amine as template, it is dissolved in ethanol solution, and adds tetraethyl orthosilicate under agitation, room temperature
Lower reaction 20h；
2) 1h is reacted respectively in 45 DEG C and 80 DEG C；
3) reaction product is placed in 1h under 90 DEG C of baking oven, obtains the hexagonal mesoporous silicon grain that size is 10~40nm.
(2) chrysanthemum cell extract is prepared
1) explant of chrysanthemum plant top children's spray section is taken, cleaning, sterilizing, is inoculated in callus inducing medium
On, induced under dark condition, temperature is that 25 DEG C of inductions produce callus cell；
Wherein, inducing culture is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), 6-benzyl aminopurine
(3.5mg/L), sucrose (20g/L) and agar (10g)；
2) by callus cell, continuous passage is bred in inducing culture, and screening cellular morphology is homogeneous, growth is stable
Chrysanthemum callus cell line；
3) step 2) is obtained into chrysanthemum callus cell line and is transferred to proliferated culture medium suspension culture, shaking table culture obtains steady
Fixed chrysanthemum suspension cell culture system；
Wherein, proliferated culture medium is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), kinetin (1.5mg/L),
Sucrose (20g/L)；Shaking speed 120rpm, 25 DEG C of temperature, is cultivated under illumination；
4) chrysanthemum suspension cell culture system is inoculated in proliferated culture medium, expands culture, obtain chrysanthemum cell；
5) proliferated culture medium after culture chrysanthemum cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes in culture medium
It is phenol red, retain culture medium in active component, and by cultivate chrysanthemum cell after proliferated culture medium and chrysanthemum cell lysate
Mixing, freeze-drying, obtain freeze-dried powder；
6) freeze-dried powder is crossed into 200 mesh sieves and obtains chrysanthemum cell extract.
(3) click part by weight and weigh each component raw material
A phases：1.2 parts of chrysanthemum cell extract, 2.0 parts of apple extract, 1.5 parts of malt extract and Rosmarinus officinalis extract
0.8 part；
B phases：3 parts of 7 parts of mesoporous silicon and monoolein；
C phases：60 parts of 4 parts of glycerine and deionized water：
D phases：2.0 parts of 3 parts of hydrogenated coco and lanolin.
(4) B phase components are thoroughly mixed, ultrasonic disperse forms blended emulsifier；
(5) A phases and C phases are thoroughly mixed to form aqueous phase in 45 DEG C of heating stirrings；
(6) D phases are heated with stirring to 80 DEG C, are thoroughly mixed to form oil phase；
(7) blended emulsifier that step (4) obtains is mixed with the oil phase that step (6) obtains, is stirring evenly and then adding into water
Xiang Zhong, it is well mixed, obtains the anti-wrinkle skin care item.
Comparative example 1
A kind of embodiment of the preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum of the invention, described in the present embodiment
The preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum is：
(1) chrysanthemum cell extract is prepared
1) explant of chrysanthemum plant top children's spray section is taken, cleaning, sterilizing, is inoculated in callus inducing medium
On, induced under dark condition, temperature is that 25 DEG C of inductions produce callus cell；
Wherein, inducing culture is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), 6-benzyl aminopurine
(3.5mg/L), sucrose (20g/L) and agar (10g)；
2) by callus cell, continuous passage is bred in inducing culture, and screening cellular morphology is homogeneous, growth is stable
Chrysanthemum callus cell line；
3) step 2) is obtained into chrysanthemum callus cell line and is transferred to proliferated culture medium suspension culture, shaking table culture obtains steady
Fixed chrysanthemum suspension cell culture system；
Wherein, proliferated culture medium is MS basal mediums, addition 1- methyl α-naphthyl acetates (0.1mg/L), kinetin (1.5mg/L),
Sucrose (20g/L)；Shaking speed 120rpm, 25 DEG C of temperature, is cultivated under illumination；
4) chrysanthemum suspension cell culture system is inoculated in proliferated culture medium, expands culture, obtain chrysanthemum cell；
5) proliferated culture medium after culture chrysanthemum cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes in culture medium
It is phenol red, retain culture medium in active component, and by cultivate chrysanthemum cell after proliferated culture medium and chrysanthemum cell lysate
Mixing, freeze-drying, obtain freeze-dried powder；
6) freeze-dried powder is crossed into 200 mesh sieves and obtains chrysanthemum cell extract.
(2) click part by weight and weigh each component raw material
A phases：1.2 parts of chrysanthemum cell extract, 2.0 parts of apple extract, 1.5 parts of malt extract and Rosmarinus officinalis extract
0.8 part；
B phases：2 parts of 3 parts of Tween-80,1 part of APG and monoolein；
C phases：50 parts of 1 part of glycerine, 2 parts of glycerine, 0.5 part of amino acid and deionized water：
D phases：1.2 parts of 5 parts of hydrogenated coco and lanolin.
(3) B phase components are thoroughly mixed, ultrasonic disperse forms blended emulsifier；
(4) A phases and C phases are thoroughly mixed to form aqueous phase in 45 DEG C of heating stirrings；
(5) D phases are heated with stirring to 80 DEG C, are thoroughly mixed to form oil phase；
(6) blended emulsifier that step (3) obtains is mixed with the oil phase that step (5) obtains, is stirring evenly and then adding into water
Xiang Zhong, it is well mixed, obtains anti-wrinkle skin care item.
Anti-wrinkle skin care item effect determines
First, it is sustained effect measure
Using anti-wrinkle skin care item prepared by embodiment 1 and comparative example 1 as experimental group and control group.Using Bag filter method
Sustained release effect of evaluation experimental group and control group, it is molten outside timing sampling detection bag filter using physiological saline as dissolution medium
The preparation of liquid general flavone, as a result as shown in table 1.
The accumulative release rate of the anti-wrinkle skin care item general flavone of table 1
| Time/h | 0 | 2 | 4 | 8 | 12 | 16 | 24 |
| Embodiment 1 | 0 | 37.6 | 46.4 | 57.8 | 66.3 | 71.1 | 74.6 |
| Comparative example 1 | 0 | 21.5 | 27.6 | 36.2 | 42.5. | 47.9 | 53.0 |
As shown in Table 1, the release of experimental group is significantly lower than control group, and mesoporous silicon passes through oxygen atom and amphipathic surface-active
The water-wet side of agent forms hydrogen bond, and layer protective layer is formed at oil-water interfaces, delays the release of anti-wrinkle active component, reaches sustained release
With the effect of long-acting wrinkle-eliminating.
2nd, Hydroxyl radical-scavenging determines
Anti-wrinkle skin care item prepared by embodiment 1~5 are configured to the aqueous solution that concentration is 50mg/mL respectively.Take 0.1mL points
Jia Ru not 0.6mL reaction buffers (deoxyribose containing 2.67mmol/L and 0.13mmol/L EDTA 0.2mol/L pH=7.4
Phosphate buffer), 0.4mmol/L ferrous sulfate 0.2mL, 2.0mmol/L ascorbic acid 0.05mL, and 20mmol/L
H2O20.05mL, 37 DEG C of warm bath 15min, 1% thiobarbituricacidα- 1mL and 2% trichloroacetic acid 1mL, boiling water bath are added after taking-up
15min, in cooled on ice after taking-up, light absorption value is surveyed under ELIASA 532nm, Scavenging action to hydroxyl free radical is calculated according to formula (2),
As a result it is as shown in table 2.
Scavenging action to hydroxyl free radical=(1- sample cells A532/ control tube A532) × 100% (2)
Table 2
Hydroxy radical is the principal element for causing biologic artifact peroxide injury as active oxygen radical.The hydroxyl of table 2
Radicals scavenging result shows that anti-wrinkle skin care item prepared by embodiment 1~5 have higher Scavenging action to hydroxyl free radical, the present invention
The scavenging action of the hydroxy radical of the anti-wrinkle skin care item of preparation and antioxidation, it is old to be advantageous to skin removing wrinkle and resisting aging.
(3) skin anti-wrinkle determines
Anti-wrinkle skin care item made from embodiment 1~5 and comparative example 1, every group is selected female of 100 ages at 35~55 years old respectively
Property volunteer, these volunteers face has that microgroove or some position wrinkles are obvious, volunteer is daily early, after late face cleaning in face
The upper anti-wrinkle skin care item smeared the present invention and prepared, are used continuously 6 months.
Evaluation criterion：Direct visual perception dermatoglyph.Effectively：Dermatoglyph substantially becomes fine and smooth, and microgroove is reduced, wrinkle
Shoal；It is invalid：Dermatoglyph and microgroove quantity do not have any change.As a result it is as shown in table 3.
Table 3
As seen from the results in Table 3, the anti-wrinkle skin care item anti-wrinkle positive effect that prepared by embodiment is better than comparative example.It is prepared by the present invention
Anti-wrinkle skin care item there is good long-acting wrinkle-eliminating effect, skin smooth can be made fine and smooth, hence it is evident that reduce wrinkle.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. the anti-wrinkle skin care item of a kind of cell extract containing chrysanthemum, it is characterised in that be made up of the component of following parts by weight：
A phases：0.5~2.0 part of chrysanthemum cell extract, 1.2~3.0 parts of apple extract, 0.6~2.5 part of malt extract and
0.1~1.8 part of Rosmarinus officinalis extract；
B phases：3~9 parts of 5~8 parts of mesoporous silicon and surfactant；
C phases：40~60 parts of 3~5 parts of NMF and deionized water；
D phases：0.5~2.0 part of 3~8 parts of hydrogenated coco and lanolin.
2. the anti-wrinkle skin care item of the cell extract according to claim 1 containing chrysanthemum, it is characterised in that by following parts by weight
Several components are made：
A phases：1.0~1.5 parts of chrysanthemum cell extract, 1.8~2.4 parts of apple extract, 1.2~2.0 parts of malt extract and
0.5~1.0 part of Rosmarinus officinalis extract；
B phases：3~9 parts of 5~8 parts of mesoporous silicon and surfactant；
C phases：40~60 parts of 3~5 parts of NMF and deionized water；
D phases：0.5~2.0 part of 3~8 parts of hydrogenated coco and lanolin.
3. the anti-wrinkle skin care item of the cell extract according to claim 1 containing chrysanthemum, it is characterised in that by following parts by weight
Several components are made：
A phases：1.2 parts of chrysanthemum cell extract, 2.0 parts of apple extract, 1.5 parts of malt extract and Rosmarinus officinalis extract 0.8
Part；
B phases：3~9 parts of 5~8 parts of mesoporous silicon and surfactant；
C phases：40~60 parts of 3~5 parts of NMF and deionized water；
D phases：0.5~2.0 part of 3~8 parts of hydrogenated coco and lanolin.
4. the anti-wrinkle skin care item of the cell extract according to claim 1 containing chrysanthemum, it is characterised in that the NMF is
At least one of glycerine, glycerine, propane diols, 1,2- butanediols, amino acid, sodium lactate, allantoin, chondroitin sulfate.
5. the anti-wrinkle skin care item of the cell extract according to claim 1 containing chrysanthemum, it is characterised in that the surface-active
Agent is at least one in Tween-80, lauric acid amide of ethanol, APG, monoolein, Emulsifier EL-60
Kind.
6. the anti-wrinkle skin care item of the cell extract according to claim 1 containing chrysanthemum, it is characterised in that the chrysanthemum cell
For chrysanthemum plant callus cell.
7. the anti-wrinkle skin care item of the cell extract according to claim 1 containing chrysanthemum, it is characterised in that the mesoporous silicon is
Hexagonal mesoporous silicon.
8. the anti-wrinkle skin care item of the cell extract according to claim 1 containing chrysanthemum, it is characterised in that the mesoporous silicon
Particle diameter is 10~40nm.
9. the preparation method of the anti-wrinkle skin care item of the cell extract containing chrysanthemum according to any one of claim 1~8, it is special
Sign is, comprises the following steps：
(1) synthesising mesoporous silicon；
(2) chrysanthemum cell extract is prepared；
(3) each component raw material is weighed in proportion；
(4) B phase components are thoroughly mixed, ultrasonic disperse forms blended emulsifier；
(5) A phases and C phases are thoroughly mixed to form aqueous phase in 45 DEG C of heating stirrings；
(6) D phases are heated with stirring to 70 DEG C~80 DEG C, are thoroughly mixed to form oil phase；
(7) blended emulsifier that step (4) obtains is mixed with the oil phase that step (6) obtains, is stirring evenly and then adding into aqueous phase,
It is well mixed, obtain the anti-wrinkle skin care item.
10. the preparation method of the anti-wrinkle skin care item of the cell extract according to claim 9 containing chrysanthemum, it is characterised in that
In the step (2), the chrysanthemum cell culture medium after culture chrysanthemum cell and the lysate of chrysanthemum cell are mixed, freezing is dry
It is dry, obtain freeze-dried powder.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020193997A1 (en) * | 2019-03-28 | 2020-10-01 | Sisaf Limited | Carrier system for preparing herbaceous extracts |
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2017
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| CN103816084A (en) * | 2014-02-28 | 2014-05-28 | 华南理工大学 | Slow- release lotion carrier for whitening and spot removing, and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020193997A1 (en) * | 2019-03-28 | 2020-10-01 | Sisaf Limited | Carrier system for preparing herbaceous extracts |
| CN113727703A (en) * | 2019-03-28 | 2021-11-30 | Sisaf有限公司 | Carrier system for preparing herbal extracts |
| AU2020249808B2 (en) * | 2019-03-28 | 2023-11-02 | Sisaf Limited | Carrier system for preparing herbaceous extracts |
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