CN107595897A - A kind of skin repair cream of the surgery containing crescent algae extract - Google Patents

Abstract

The invention discloses a kind of skin repair cream of the surgery containing crescent algae extract, added with Closterium extract solution, it is combined with stearic acid, glycerin monostearate, hexadecanol, octadecyl alcolol, glycerine, potassium hydroxide and polysorbate60 and distilled water, essence 0.5% and preservative composition, wherein crescent algae extract is fermented by Closterium, high pressure broken wall, breaking wall by fermentation, working substance decomposing and fermenting, the step such as post processing and extract solution concentration is prepared, obtained skin repair cream contains Closterium albumen, polysaccharide, a variety of fermentation activity materials such as natural bata-carotene and microalgae oil, maintenance and antibacterial effect to skin and scalp injury is obvious.

Description

A kind of skin repair cream of the surgery containing crescent algae extract

Technical field

The invention belongs to field of biological extraction, and in particular to a kind of surgery skin repair containing crescent algae extract Cream.

Background technology

Skin repair cream refers to skin injury, including the external used medicine that symptom is treated such as scalds, tans severely, burning. Products propaganda in the market on protecting and repairing skin is powerful as many as hundreds of, and these products are mostly with skin Damage under not serious situation and use, it is impossible to reply skin problem more caused by rugged environment, just because of wherein chemical Material is more, and natural products is less.Even and if a small number of skin repair cream is added with natural materials, also mostly because big point of biology Son is not easy to absorb and limited efficacy, or even has producer to realize short run effect addition mineral oil, alcohol, silicon and hormone etc. pair The harmful material of skin.

Closterium is unicellular organism, is algae.Cell is crescent, and there is a core in center, and core both sides respectively have a leaf green Body.There is vertical strip projection on the surface of chloroplaset, and cross section is in aristiform；There are 1 row to make powder core in chloroplaset.Cell wall is smooth or tool is vertical To strain line, rib line or point line, it is colourless or because molysite deposit and be in yellowish-brown.Chromatoplast axle position, each 1 in two semicells, tool Longitudinal ridge, pyrenoids axis one arrange or scattered.The middle part of nucleus cell between two chromatoplasts.Each tool one is big at the ends of the cell Type vacuole, wherein containing one or more gypsum crystal grain（Constantly trembleed in living cells）.Cell both ends have hole, secreting outside Colloid causes signaling.During breeding, cell division is each gradually grown another half-fine with itself identical into 1 semicell Born of the same parents, annular suture are often left on the cell membrane of half-fine intercellular newly formed and female, the suture of some species is not located at the near of cell Middle part, but claim annulus in other positions, part therebetween.

Closterium secures a large amount of albumen, wherein obvious containing substantial amounts of microalgae albumen, polysaccharides and antioxidation Beta carotene and microalgae oil, there is good maintenance action to human body, but in the inhibition of microalgae cell wall and microalgae The macromolecular structure of albumen and polysaccharide, is unfavorable for the absorption of human epidermal, therefore finds more efficient thoroughly microalgae cell wall broken wall, Microalgae cell nutrients extracting and developing and the good method of decomposition, and it is combined with skin repair cream, turn at this stage urgently Problem to be broken through.

The content of the invention

The present invention is in order to solve that skin repair cream feature at this stage is single, the addition of chemical noxious material is more and to skin The reparation badly damaged do not apply to, it is poor to act on, and Closterium cell membrane is broken not thoroughly, and intracellular macromolecular substances are unfavorable for people Body absorb the shortcomings that and propose, both are combined, it is proposed that a kind of surgery containing crescent algae extract is repaiied with skin Multiple cream.

To achieve the above object, the present invention provides following technical scheme：A kind of surgery skin containing crescent algae extract Skin repairing paste, it is prepared according to percentage by weight by following steps,

（1）Weigh stearic acid 15%, glycerin monostearate 2%, hexadecanol 0.5%, octadecyl alcolol 0.5% to be added in jacketed pan, add Heat is melted to 90 DEG C and stirring and dissolving is standby；

Glycerin monostearate is surfactant, can keep the moisture of skin and have preferable use feeling, with other materials The oil phase base soln of repairing paste is obtained after mixed dissolution.

（2）4.5% glycerine, 0.5% potassium hydroxide and 1% polysorbate60 are added in 70% distilled water be added to it is another In jacketed pan, it is heated to 90 DEG C and stirs；

Polysorbate60 is another surfactant, and the appearance retention of clean-up performance and product is preferable, can effectively prevent The phenomenon of precipitated and separated, it is subjected to Hybrid Heating dissolving with glycerine etc., obtains the aqueous phase base soln of repairing paste.

（3）By step（1）And step（2）50 DEG C are cooled to after obtained solution mixing, adds Closterium extract solution 5%, perfume Essence 0.5% and the stirring and dissolving 1-2 hours of preservative 0.5%, it is cooled to 30 DEG C of bottlings and obtains surgery skin repair cream；

By step（2）In aqueous phase solution be added to step（1）In oil-phase solution in, add repairing paste other compounding materials And essence, improve the physical behavior of repairing paste, and add Closterium extract solution be sufficiently stirred dissolving after can obtain containing newly The surgical skin repairing paste of month algae extract.

Step（3）In Closterium extract solution be prepared by following steps,

a）Closterium ferments：It will be fermented in 5% Closterium access Closterium fermentation liquid culture medium, by frond quantity Monitoring, frond quantity reaches maximum after 92-100 hours of fermenting；

Closterium is expanded by illumination cultivation in sea water in microalgae, expands culture in fermentation system is flowed in illumination, often Frond quantity reaches maximum after the detection that 2 hours carry out frond quantity, 92-100 hours of fermenting, using blood counting chamber method Method as frond detection.

b）High pressure broken wall：The Closterium zymotic fluid that step a is obtained is pumped into high pressure homogenizer and carries out high-pressure homogenization, is controlled Operating pressure 70MPa, homogenized natural cooling after 15 minutes；

Closterium zymotic fluid after fermentation is subjected to high-pressure homogenization processing together with culture medium using high pressure homogenizer, control is suitable In operating pressure and Homogenization time, by microscopy, Closterium reduction ratios reach more than 90%.Control homogenized temperature is no more than 15 minutes, overlong time or cause temperature too high so that macromolecular microalgae protein inactivation, the time, too short then percentage of damage was relatively low.

High-pressure homogenization becomes apparent from than current other breaking method effects, and traditional swelling method needs small in dark condition Processing more than 24 hours, overlong time, and the power consumption of multigelation method is excessive, and chlorination calcium impurities are introduced, it is unfavorable for later stage production The purification and utilization of thing.

c）Breaking wall by fermentation：In Closterium fermentation liquid pump human hair fermentation tank after broken wall is cooled down, the sugarcane of mass percent 3% is added After sugar, 0.3% sodium nitrate, 0.05% magnesium sulfate, 0.05% potassium chloride and 1% dipotassium hydrogen phosphate stirring and dissolving, 2% aspergillus niger is accessed Bacterium solution, after 24-30 hours of being fermented at 28 DEG C, fermentation jar temperature is promoted to 121 DEG C, insulation puts tank after 20 minutes, naturally cold But；

Other carbon sources, nitrogen source and inorganic salts are added in the zymotic fluid after broken, access aspergillus niger is fermented, aspergillus niger hair Ferment can produce substantial amounts of cellulase and a small amount of protease and pectase, and high-pressure homogenization can be broken wall by cellulase Cell is further processed, with can also divide broken Closterium cell membrane under the collective effect of protease and pectase Solution is into micromolecular polysaccharide, the abundant decomposition and utilization of the organic matter reached.

d）Working substance decomposing and fermenting：Liquid after being cooled down in step c is pumped into fermentation tank again, is respectively connected to 1.5% Bacillus amyloliquefaciens and 2% solution curdled milk series bacillus, fermented at 32 DEG C after 18-20 hours, improve fermentation jar temperature extremely 121 DEG C, insulation puts tank, natural cooling after 20 minutes；

The bacillus amyloliquefaciens that can largely produce protease are added in thoroughly broken frond and produce polysaccharide hydrolase Solution curdled milk series bacillus, caused protease and polysaccharide hydrolase, the high molecular weight protein in frond and carbohydrate can be entered Water-filling solution, produce polypeptide and small molecule carbohydrate that small molecule is beneficial to absorption of human body.

e）Post processing：After liquid after step d is cooled down is by 4 layers of filtered through gauze, 5000 revs/min of low speed centrifuge is used After Zhongli's heart 25 minutes, take supernatant standby；

By the liquid obtained in step d by filtered through gauze and centrifugation after, remove most thalline and a small amount of unbroken micro- Frustule.

f）Extract solution concentrates：The liquid obtained in step e is subjected to low pressure rotary evaporation, volume contracting in Rotary Evaporators Stop evaporation after the as low as 5%-10% of original volume, final Closterium extract solution is obtained after cooling.

The liquid obtained in step e is subjected to low pressure rotary evaporation, under low-pressure low-temperature processing be concentrated to give final new Month algae extract solution.

Preferably, the Closterium fermentation liquid culture medium in the Closterium extract solution preparation process a is by following weight Material composition, 50 milligrams of sodium nitrate, 5 milligrams of potassium dihydrogen phosphate, 0.1 milligram of ferric sulfate, 10 milligrams of sodium citrate, the milli of sodium metasilicate 10 Gram, the microgram of vitamin B12 0.2,1000 grams of seawater.

Preferably, the aspergillus niger in the Closterium extract solution preparation process c is purchased from Chinese industrial Microbiological Culture Collection Administrative center, preserving number：CICC40273；Bacillus amyloliquefaciens reconciliation curdled milk series bacillus is obtained in the step d to be purchased from Chinese industrial Microbiological Culture Collection administrative center, preserving number are respectively：CICC10160、CICC10358.

Compared with prior art, the beneficial effects of the invention are as follows：Contain Closterium extract solution in Closterium skin repair cream, Contain a large amount of frond albumen in Closterium, Closterium frond protein content reaches more than 60%, also containing substantial amounts of microalgae grease and Polysaccharides, and contain natural beta-carotin, there is maintenance and antibacterial action well to body, have and suppress tumour, anti-spoke Penetrate the effect with increasing leukocyte.And Closterium adds bioanalysis to crush frond using physics, crushing effect is notable, using a variety of micro- Biological fermentation process decomposes high molecular weight protein and polysaccharide, has mild condition, reacts the advantages of easily-controllable, and new with bioanalysis production Month algae extract solution, has environment-friendly free of contamination remarkable advantage, almost notable without the discharge of discarded object, ecological benefits.

Embodiment

The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment Only part of the embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area The every other embodiment that art personnel are obtained under the premise of creative work is not made, belong to the model that the present invention protects Enclose.

The present invention provides a kind of technical scheme：A kind of skin repair cream of the surgery containing crescent algae extract, according to weight Amount percentage is prepared by following steps:

（1）Weigh stearic acid 15%, glycerin monostearate 2%, hexadecanol 0.5%, octadecyl alcolol 0.5% to be added in jacketed pan, add Heat is melted to 90 DEG C and stirring and dissolving is standby；

（2）4.5% glycerine, 0.5% potassium hydroxide and 1% polysorbate60 are added in 70% distilled water and are added to another interlayer In pot, it is heated to 90 DEG C and stirs；

（3）By step（1）And step（2）50 DEG C are cooled to after obtained solution mixing, adds Closterium extract solution 5%, essence 0.5% and the stirring and dissolving 1-2 hours of preservative 0.5%, it is cooled to 30 DEG C of bottlings and obtains surgery skin repair cream；

Wherein step（3）In Closterium extract solution be prepared by following steps：

a）Closterium ferments：It will be fermented in 5% Closterium access Closterium fermentation liquid culture medium, by frond quantity Monitoring, frond quantity reaches maximum after 92-100 hours of fermenting；

b）High pressure broken wall：The Closterium zymotic fluid that step a is obtained is pumped into high pressure homogenizer and carries out high-pressure homogenization, control operation Pressure 70MPa, homogenized natural cooling after 15 minutes；

c）Breaking wall by fermentation：In Closterium fermentation liquid pump human hair fermentation tank after broken wall is cooled down, addition mass percent 3% sucrose, After 0.3% sodium nitrate, 0.05% magnesium sulfate, 0.05% potassium chloride and 1% dipotassium hydrogen phosphate stirring and dissolving, 2% black-koji mould is accessed Liquid, after 24-30 hours of being fermented at 28 DEG C, fermentation jar temperature is promoted to 121 DEG C, insulation puts tank, natural cooling after 20 minutes；

d）Working substance decomposing and fermenting：Liquid after being cooled down in step c is pumped into fermentation tank again, is respectively connected to 1.5% Xie Dian Afnyloliquefaciens and 2% solution curdled milk series bacillus, after 18-20 hours of being fermented at 32 DEG C, fermentation jar temperature is improved to 121 DEG C, insulation puts tank, natural cooling after 20 minutes；

e）Post processing：After liquid after step d is cooled down is by 4 layers of filtered through gauze, using 5000 revs/min of low speed centrifuge from After the heart 25 minutes, take supernatant standby；

f）Extract solution concentrates：The liquid obtained in step e is subjected to low pressure rotary evaporation in Rotary Evaporators, volume-diminished is extremely Stop evaporation after the 5%-10% of original volume, final Closterium extract solution is obtained after cooling.

Closterium fermentation liquid culture medium in Closterium extract solution preparation process a is by the material composition of following weight, nitre Sour 50 milligrams of sodium, 5 milligrams of potassium dihydrogen phosphate, 0.1 milligram of ferric sulfate, 10 milligrams of sodium citrate, 10 milligrams of sodium metasilicate, vitamin 1000 grams of B120.2 micrograms, seawater.

Aspergillus niger in Closterium extract solution preparation process c is purchased from Chinese industrial Microbiological Culture Collection administrative center, protects Tibetan number：CICC40273；In the step d bacillus amyloliquefaciens conciliate curdled milk series bacillus to be purchased from Chinese industrial micro- Biological inoculum preservation administrative center, preserving number are respectively：CICC10160、CICC10358.

Embodiment 1：

By 5 grams of sodium nitrate, 0.5 gram of potassium dihydrogen phosphate, 10 milligrams of ferric sulfate, 1 gram of sodium citrate, 1 gram of sodium metasilicate, the dimension life of 20 micrograms Plain B12 is dissolved in the seawater of double centner, and fermented and cultured is after 92 hours in illumination reaction device for 5 kilograms of Closteriums of access, frond Quantity reaches maximum；By frond ferment liquid pump people's high pressure homogenizer in, control pressure homogenized 15 minutes under the conditions of 70MPa After be pumped into fermentation tank；Add 3 kilograms of sucrose, 0.3 kilogram of sodium nitrate, 0.05 kilogram of magnesium sulfate, 0.05 kilogram of potassium chloride and 1 Kilogram dipotassium hydrogen phosphate stirring and dissolving, 2 kilograms of aspergillus niger bacterium solutions are accessed, after being fermented 24 hours at 28 DEG C, temperature is promoted to 121 DEG C insulation 20 minutes after put tank natural cooling；Liquid is pumped into fermentation tank again, the solution starch gemma bar of 1.5 kilograms of access Bacterium and 2 kilograms of solution curdled milk series bacillus, after being fermented 18 hours at 32 DEG C, fermentation jar temperature are improved to 121 DEG C, insulation 20 Tank, natural cooling are put after minute；After liquid is by 4 layers of filtered through gauze, with low speed centrifuge, 5000 revs/min centrifuge 25 minutes, Take supernatant；Liquid carries out low pressure rotary evaporation in Rotary Evaporators, volume-diminished to original volume 5% after stop evaporation, it is cold But 5 kilograms of final Closterium extract solution is obtained afterwards.

Weigh 0.5 kilogram of 15 kilograms of stearic acid, 2 kilograms of glycerin monostearate, 0.5 kilogram of hexadecanol, octadecyl alcolol addition Into jacketed pan, it is heated to 90 DEG C of thawings and stirring and dissolving is standby, obtain repairing paste oil phase base soln；In another jacketed pan 4.5 kilograms of glycerine of middle addition, 0.5 kilogram of potassium hydroxide and 1 kilogram of polysorbate60 are added in 70 kilograms of distilled water, are heated To 90 DEG C and stir, obtain repairing paste aqueous phase base soln；Obtained aqueous phase base soln solution is added to oil phase base 50 DEG C are cooled to after being mixed in plinth solution, 0.5 kilogram of 5 kilograms of Closterium extract solution, 0.5 kilogram of essence and preservative is added and stirs Dissolving 1-2 hours are mixed, 30 DEG C of bottlings is cooled to and obtains surgery skin repair cream double centner.

Embodiment 2：

By 15 grams of sodium nitrate, 1.5 grams of potassium dihydrogen phosphates, 30 milligrams of ferric sulfate, 3 grams of sodium citrates, 3 grams of sodium metasilicate, 60 micrograms dimension Raw plain B12 is dissolved in 300 kilograms of seawater, 15 kilograms of Closteriums of access fermented and cultured after 100 hours in illumination reaction device, Frond quantity reaches maximum；By frond ferment liquid pump people's high pressure homogenizer in, control pressure homogenized 15 under the conditions of 70MPa It is pumped into after minute in fermentation tank；Add 9 kilograms of sucrose, 0.9 kilogram of sodium nitrate, 0.15 kilogram of magnesium sulfate, 0.15 kilogram of potassium chloride And 3 kilograms of dipotassium hydrogen phosphate stirring and dissolvings, 6 kilograms of aspergillus niger bacterium solutions are accessed, after being fermented 30 hours at 28 DEG C, temperature is carried Rise to 121 DEG C insulation 20 minutes after put tank natural cooling；Liquid is pumped into fermentation tank again, the solution starch bud of 4.5 kilograms of access Spore bacillus and 6 kilograms of solution curdled milk series bacillus, after being fermented 20 hours at 32 DEG C, fermentation jar temperature are improved to 121 DEG C, is protected Temperature puts tank, natural cooling after 20 minutes；After liquid is by 4 layers of filtered through gauze, with low speed centrifuge, 5000 revs/min centrifuge 25 points Clock, take supernatant；Liquid carries out low pressure rotary evaporation in Rotary Evaporators, volume-diminished to original volume 10% after stop steaming Hair, obtains 30 kilograms of final Closterium extract solution after cooling.

Weigh 90 kilograms of stearic acid, 12 kilograms of glycerin monostearate, 3 kilograms of hexadecanol, 3 kilograms of octadecyl alcolol and be added to folder In layer pot, it is heated to 90 DEG C of thawings and stirring and dissolving is standby, obtain repairing paste oil phase base soln；Add in another jacketed pan Enter 27 kilograms of glycerine, 3 kilograms of potassium hydroxide and 6 kilograms of polysorbate60 to be added in 420 kilograms of distilled water, be heated to 90 DEG C And stir, obtain repairing paste aqueous phase base soln；Obtained aqueous phase base soln solution is added to oil phase base soln 50 DEG C are cooled to after middle mixing, adds 3 kilograms of 30 kilograms of Closterium extract solution, 3 kilograms of essence and preservative stirring and dissolving 1-2 Hour, it is cooled to 30 DEG C of bottlings and obtains 600 kilograms of surgery skin repair cream.

Experimental example 1：

External minimum inhibitory concentration MIC experiments：

Test strain：Fungi, Candida albicans, staphylococcus aureus, enteropathogenic E. Coli.

Method：Bacteria suspension is made with physiological saline in each test strain, and it is more than 2.0McF bacteria suspensions to be diluted to turbidity, Again with after normal saline dilution, RPMIi640 nutrient solutions dilute, test group（Skin repair cream in the embodiment of the present invention）With it is right According to group（Common skin repairing paste）Doubling dilution, cultivate 80 hours in 96 well culture plates, slight concussion, 490nm places progress photoelectricity Colorimetric,

As can be seen that the skin repair cream fungistatic effect of the present invention is obvious from experiment, to most of kind bacteroid in skin There is inhibitory action.

Experimental example 2：

The skin repair cream in the embodiment of the present invention 1, embodiment 2 and common repairing paste and blank control group is taken to be tested, Occupation mode is daily 1-2 times, and each 3-5 grams, most long use 8 weeks, wherein Symptoms Assessment are by staff before use, 4 weeks Assessed with 8 weeks, symptom feature：The overall severity that drying, husking, erythema, trace are itched, wherein according to seriously from low to high It is divided into 0-3 levels,

Group	Overall degree（0 day）	Overall degree（4 weeks）	Overall degree（8 weeks）
				Embodiment 1	2.5	1.5	0.6
Embodiment 2	2.3	1.6	0.5
				Common repairing paste	2.4	2	1.8
Control group	2.6	2.5	2.6

From experimental result it can be seen that the skin repair product of the embodiment of the present invention has for the various problems of skin well repaiies Multiple effect.

Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (3)

1. A kind of 1. skin repair cream of the surgery containing crescent algae extract, it is characterised in that：According to percentage by weight by following Step is prepared,

   （1）Weigh stearic acid 15%, glycerin monostearate 2%, hexadecanol 0.5%, octadecyl alcolol 0.5% to be added in jacketed pan, add Heat is melted to 90 DEG C and stirring and dissolving is standby；

   （2）4.5% glycerine, 0.5% potassium hydroxide and 1% polysorbate60 are added in 70% distilled water and are added to another interlayer In pot, it is heated to 90 DEG C and stirs；

   （3）By step（1）And step（2）50 DEG C are cooled to after obtained solution mixing, adds Closterium extract solution 5%, essence 0.5% and the stirring and dissolving 1-2 hours of preservative 0.5%, it is cooled to 30 DEG C of bottlings and obtains surgery skin repair cream；

   The step（3）In Closterium extract solution be prepared by following steps,

   (a) Closterium ferments：It will be fermented in 5% Closterium access Closterium fermentation liquid culture medium, by frond quantity Monitoring, frond quantity reaches maximum after 92-100 hours of fermenting；

   (b) high pressure broken wall：The Closterium zymotic fluid that step a is obtained is pumped into high pressure homogenizer and carries out high-pressure homogenization, control behaviour Make pressure 70MPa, homogenized natural cooling after 15 minutes；

   (c) breaking wall by fermentation：In Closterium fermentation liquid pump human hair fermentation tank after broken wall is cooled down, addition mass percent 3% sucrose, After 0.3% sodium nitrate, 0.05% magnesium sulfate, 0.05% potassium chloride and 1% dipotassium hydrogen phosphate stirring and dissolving, 2% black-koji mould is accessed Liquid, after 24-30 hours of being fermented at 28 DEG C, fermentation jar temperature is promoted to 121 DEG C, insulation puts tank, natural cooling after 20 minutes；

   (d) working substance decomposing and fermenting：Liquid after being cooled down in step c is pumped into fermentation tank again, is respectively connected to 1.5% solution Bacillus amyloliquefacienses and 2% solution curdled milk series bacillus, fermented at 32 DEG C after 18-20 hours, improve fermentation jar temperature extremely 121 DEG C, insulation puts tank, natural cooling after 20 minutes；

   (e) post-process：After liquid after step d is cooled down is by 4 layers of filtered through gauze, 5000 revs/min of low speed centrifuge is used After centrifugation 25 minutes, take supernatant standby；

   （f）Extract solution concentrates：The liquid obtained in step e is subjected to low pressure rotary evaporation, volume-diminished in Rotary Evaporators Stop evaporation after to the 5%-10% of original volume, final Closterium extract solution is obtained after cooling.
2. A kind of 2. skin repair cream of the surgery containing crescent algae extract according to claim 1, it is characterised in that：Institute The Closterium fermentation liquid culture medium stated in Closterium extract solution preparation process a is by the material composition of following weight, sodium nitrate 50 Milligram, 5 milligrams of potassium dihydrogen phosphate, 0.1 milligram of ferric sulfate, 10 milligrams of sodium citrate, 10 milligrams of sodium metasilicate, vitamin B12 0.2 are micro- Gram, 1000 grams of seawater.
3. A kind of 3. skin repair cream of the surgery containing crescent algae extract according to claim 1, it is characterised in that：Institute The aspergillus niger stated in Closterium extract solution preparation process c is purchased from Chinese industrial Microbiological Culture Collection administrative center, preserving number： CICC40273；Bacillus amyloliquefaciens reconciliation curdled milk series bacillus is obtained in the step d and is purchased from Chinese industrial microbial bacteria Kind preservation administrative center, preserving number are respectively：CICC10160、CICC10358.