CN107496219A - A kind of Closterium facial treatment mask - Google Patents
A kind of Closterium facial treatment mask Download PDFInfo
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- CN107496219A CN107496219A CN201710782780.3A CN201710782780A CN107496219A CN 107496219 A CN107496219 A CN 107496219A CN 201710782780 A CN201710782780 A CN 201710782780A CN 107496219 A CN107496219 A CN 107496219A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Dermatology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Veterinary Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of Closterium facial treatment mask, added with Closterium extract solution, it is combined with polyvinyl alcohol, sodium alginate, carboxymethyl cellulose, propane diols, glycerine, silicon breast, ethanol, sodium benzoate, essence is dissolved in deionized water and formed, facial treatment mask liquid is added in the non-woven fabrics of aluminium foil bag and sealed, obtain Closterium facial treatment mask, wherein crescent algae extract is fermented by Closterium, high pressure broken wall, breaking wall by fermentation, working substance decomposing and fermenting, the step such as post processing and extract solution concentration is prepared, obtained facial mask contains Closterium albumen, polysaccharide, a variety of fermentation activity materials such as natural bata-carotene and microalgae oil, maintenance and antibacterial effect to face skin is obvious.
Description
Technical field
The invention belongs to field of biological extraction, and in particular to a kind of Closterium facial treatment mask.
Background technology
Facial mask, it is a classification in skin care item.Its most basic and most important purpose is to make up makeup removing and wash one's face still
So cleaning of deficiency, coordinates other essential ingredients to realize other maintenance functions, such as replenishing water and preserving moisture, U.S. on this basis
In vain, anti-aging, Sage Extract etc..The principle of facial mask, exactly using the of short duration time for being covered in face, it is temporarily isolating the external world
Air and pollution, skin temperature is improved, the pore expansion of skin, promotes sweat gland secretion and metabolism, makes the oxygen content of skin
Rise, the oil substances for being advantageous to the product of skin exclusion epidermal cell metabolism and accumulating, the moisture in facial mask penetrates into
The cuticula of epidermis, skin become soft, and skin is naturally bright flexible.
Closterium is unicellular organism, is algae.Cell is crescent, and there is a core in center, and core both sides respectively have a leaf green
Body.There is vertical strip projection on the surface of chloroplaset, and cross section is in aristiform；There are 1 row to make powder core in chloroplaset.Cell wall is smooth or tool is vertical
To strain line, rib line or point line, it is colourless or because molysite deposit and be in yellowish-brown.Chromatoplast axle position, each 1 in two semicells, tool
Longitudinal ridge, pyrenoids axis one arrange or scattered.The middle part of nucleus cell between two chromatoplasts.Each tool one is big at the ends of the cell
Type vacuole, wherein containing one or more gypsum crystal grain（Constantly trembleed in living cells）.Cell both ends have hole, secreting outside
Colloid causes signaling.During breeding, cell division is each gradually grown another half-fine with itself identical into 1 semicell
Born of the same parents, annular suture are often left on the cell membrane of half-fine intercellular newly formed and female, the suture of some species is not located at the near of cell
Middle part, but claim annulus in other positions, part therebetween.
Closterium secures a large amount of albumen, wherein obvious containing substantial amounts of microalgae albumen, polysaccharides and antioxidation
Beta carotene and microalgae oil, there is good maintenance action to human body, but in the inhibition of microalgae cell wall and microalgae
The macromolecular structure of albumen and polysaccharide, is unfavorable for the absorption of human epidermal, therefore finds more efficient thoroughly microalgae cell wall broken wall,
Microalgae cell nutrients extracting and developing and the good method of decomposition, and it is combined with facial mask, turn into and urgently break through at this stage
Problem.
The content of the invention
The present invention is single and poor to facial skin care and health-care effect in order to solve facial mask feature at this stage, and newly
The shortcomings that month frustule wall is broken not thoroughly, and intracellular macromolecular substances are unfavorable for absorption of human body and propose, both are tied
Close, it is proposed that a kind of Closterium facial treatment mask.
To achieve the above object, the present invention provides following technical scheme：A kind of Closterium facial treatment mask, according to weight percent
Than being prepared by following steps,
（1）0.1% sodium benzoate, 1% sodium alginate and 3% carboxymethyl cellulose is weighed to be dissolved in 65% deionized water,
It is standby to be sufficiently stirred dissolving；
Sodium alginate and carboxymethyl cellulose dissolve among deionized water, have good thickening power, add benzene first
Sour sodium preservative is dissolved, and obtains the base soln of facial mask.
（2）In 13% polyvinyl alcohol step will be added to after the ethanol wet of addition 9%（1）In solution in be heated to 70
DEG C, constantly it is stirred, is allowed to well mixed, stands overnight；
Ethanol has the function that so that polyvinyl alcohol increase-volume, also may be such that overnight facial mask base soln more in a heated condition
Sufficiently dissolving.
（3）1% propane diols, 3% glycerine, 1% silicon breast, 4% Closterium extract solution and 0.1% essence are added into step（2）
To solution in, be sufficiently stirred dissolving both Closterium facial treatment mask liquid；
Add facial mask surfactant silicon breast and wait other compounding materials and essence, improve the physical behavior of facial mask liquid, and add
Closterium extract solution can obtain Closterium facial mask liquid after being sufficiently stirred dissolving.
（4）It is put into after non-woven fabrics is folded in aluminium foil bag, adds step（3）Closterium facial treatment mask liquid 20-50 milliliters
After seal, obtain Closterium facial treatment mask.
Closterium facial mask liquid is fully absorbed using non-woven fabrics, according to facial mask size and non-woven fabrics material different choice not
Same addition, obtains Closterium facial treatment mask after sealing.
Step（3）In Closterium extract solution be prepared by following steps,
a）Closterium ferments：It will be fermented in 5% Closterium access Closterium fermentation liquid culture medium, by frond quantity
Monitoring, frond quantity reaches maximum after 92-100 hours of fermenting；
Closterium is expanded by illumination cultivation in sea water in microalgae, expands culture in fermentation system is flowed in illumination, often
Frond quantity reaches maximum after the detection that 2 hours carry out frond quantity, 92-100 hours of fermenting, using blood counting chamber method
Method as frond detection.
b）High pressure broken wall：The Closterium zymotic fluid that step a is obtained is pumped into high pressure homogenizer and carries out high-pressure homogenization, is controlled
Operating pressure 70MPa, homogenized natural cooling after 15 minutes；
Closterium zymotic fluid after fermentation is subjected to high-pressure homogenization processing together with culture medium using high pressure homogenizer, control is suitable
In operating pressure and Homogenization time, by microscopy, Closterium reduction ratios reach more than 90%.Control homogenized temperature is no more than
15 minutes, overlong time or cause temperature too high so that macromolecular microalgae protein inactivation, the time, too short then percentage of damage was relatively low.
High-pressure homogenization becomes apparent from than current other breaking method effects, and traditional swelling method needs small in dark condition
Processing more than 24 hours, overlong time, and the power consumption of multigelation method is excessive, and chlorination calcium impurities are introduced, it is unfavorable for later stage production
The purification and utilization of thing.
c）Breaking wall by fermentation：In Closterium fermentation liquid pump human hair fermentation tank after broken wall is cooled down, the sugarcane of mass percent 3% is added
After sugar, 0.3% sodium nitrate, 0.05% magnesium sulfate, 0.05% potassium chloride and 1% dipotassium hydrogen phosphate stirring and dissolving, 2% aspergillus niger is accessed
Bacterium solution, after 24-30 hours of being fermented at 28 DEG C, fermentation jar temperature is promoted to 121 DEG C, insulation puts tank after 20 minutes, naturally cold
But；
Other carbon sources, nitrogen source and inorganic salts are added in the zymotic fluid after broken, access aspergillus niger is fermented, aspergillus niger hair
Ferment can produce substantial amounts of cellulase and a small amount of protease and pectase, and high-pressure homogenization can be broken wall by cellulase
Cell is further processed, with can also divide broken Closterium cell membrane under the collective effect of protease and pectase
Solution is into micromolecular polysaccharide, the abundant decomposition and utilization of the organic matter reached.
d）Working substance decomposing and fermenting：Liquid after being cooled down in step c is pumped into fermentation tank again, is respectively connected to 1.5%
Bacillus amyloliquefaciens and 2% solution curdled milk series bacillus, fermented at 32 DEG C after 18-20 hours, improve fermentation jar temperature extremely
121 DEG C, insulation puts tank, natural cooling after 20 minutes；
The bacillus amyloliquefaciens that can largely produce protease are added in thoroughly broken frond and produce polysaccharide hydrolase
Solution curdled milk series bacillus, caused protease and polysaccharide hydrolase, the high molecular weight protein in frond and carbohydrate can be entered
Water-filling solution, produce polypeptide and small molecule carbohydrate that small molecule is beneficial to absorption of human body.
e）Post processing：After liquid after step d is cooled down is by 4 layers of filtered through gauze, 5000 revs/min of low speed centrifuge is used
After Zhongli's heart 25 minutes, take supernatant standby；
By the liquid obtained in step d by filtered through gauze and centrifugation after, remove most thalline and a small amount of unbroken micro-
Frustule.
f）Extract solution concentrates：The liquid obtained in step e is subjected to low pressure rotary evaporation, volume contracting in Rotary Evaporators
Stop evaporation after the as low as 5%-10% of original volume, final Closterium extract solution is obtained after cooling.
The liquid obtained in step e is subjected to low pressure rotary evaporation, under low-pressure low-temperature processing be concentrated to give final new
Month algae extract solution.
Preferably, the Closterium fermentation liquid culture medium in the Closterium extract solution preparation process a is by following weight
Material composition, 50 milligrams of sodium nitrate, 5 milligrams of potassium dihydrogen phosphate, 0.1 milligram of ferric sulfate, 10 milligrams of sodium citrate, the milli of sodium metasilicate 10
Gram, the microgram of vitamin B12 0.2,1000 grams of seawater.
Preferably, the aspergillus niger in the Closterium extract solution preparation process c is purchased from Chinese industrial Microbiological Culture Collection
Administrative center, preserving number：CICC40273；Bacillus amyloliquefaciens reconciliation curdled milk series bacillus is obtained in the step d to be purchased from
Chinese industrial Microbiological Culture Collection administrative center, preserving number are respectively：CICC10160、CICC10358.
Compared with prior art, the beneficial effects of the invention are as follows：Contain Closterium extract solution, Closterium in Closterium facial mask
In contain a large amount of frond albumen, Closterium frond protein content reaches more than 60%, also more containing substantial amounts of microalgae grease and algae
Sugar, and contain natural beta-carotin, there is maintenance and antibacterial action well to body, have suppress tumour, radioresistance and
The effect of increasing leukocyte.And Closterium adds bioanalysis to crush frond using physics, crushing effect is notable, using multiple-microorganism
Fermentation method decomposes high molecular weight protein and polysaccharide, has mild condition, reacts the advantages of easily-controllable, and the Closterium produced with bioanalysis
Extract solution, there is environment-friendly free of contamination remarkable advantage, it is almost notable without the discharge of discarded object, ecological benefits.
Embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment that art personnel are obtained under the premise of creative work is not made, belong to the model that the present invention protects
Enclose.
The present invention provides a kind of technical scheme：A kind of Closterium facial treatment mask, according to percentage by weight by following steps system
It is standby to form:
（1）0.1% sodium benzoate, 1% sodium alginate and 3% carboxymethyl cellulose is weighed to be dissolved in 65% deionized water,
It is standby to be sufficiently stirred dissolving；
（2）In 13% polyvinyl alcohol step will be added to after the ethanol wet of addition 9%（1）In solution in be heated to 70 DEG C,
Constantly it is stirred, is allowed to well mixed, stands overnight；
（3）1% propane diols, 3% glycerine, 1% silicon breast, 4% Closterium extract solution and 0.1% essence are added into step（2）Obtain
In solution, it is sufficiently stirred dissolving and both obtains Closterium facial treatment mask liquid；
（4）It is put into after non-woven fabrics is folded in aluminium foil bag, adds step（3）Closterium facial treatment mask liquid 20-50 milliliters after seal
Mouthful, obtain Closterium facial treatment mask；
Wherein step（3）In Closterium extract solution be prepared by following steps：
a）Closterium ferments：It will be fermented in 5% Closterium access Closterium fermentation liquid culture medium, by frond quantity
Monitoring, frond quantity reaches maximum after 92-100 hours of fermenting；
b）High pressure broken wall：The Closterium zymotic fluid that step a is obtained is pumped into high pressure homogenizer and carries out high-pressure homogenization, control operation
Pressure 70MPa, homogenized natural cooling after 15 minutes；
c）Breaking wall by fermentation：In Closterium fermentation liquid pump human hair fermentation tank after broken wall is cooled down, addition mass percent 3% sucrose,
After 0.3% sodium nitrate, 0.05% magnesium sulfate, 0.05% potassium chloride and 1% dipotassium hydrogen phosphate stirring and dissolving, 2% black-koji mould is accessed
Liquid, after 24-30 hours of being fermented at 28 DEG C, fermentation jar temperature is promoted to 121 DEG C, insulation puts tank, natural cooling after 20 minutes；
d）Working substance decomposing and fermenting：Liquid after being cooled down in step c is pumped into fermentation tank again, is respectively connected to 1.5% Xie Dian
Afnyloliquefaciens and 2% solution curdled milk series bacillus, after 18-20 hours of being fermented at 32 DEG C, fermentation jar temperature is improved to 121
DEG C, insulation puts tank, natural cooling after 20 minutes；
e）Post processing：After liquid after step d is cooled down is by 4 layers of filtered through gauze, using 5000 revs/min of low speed centrifuge from
After the heart 25 minutes, take supernatant standby；
f）Extract solution concentrates：The liquid obtained in step e is subjected to low pressure rotary evaporation in Rotary Evaporators, volume-diminished is extremely
Stop evaporation after the 5%-10% of original volume, final Closterium extract solution is obtained after cooling.
Closterium fermentation liquid culture medium in Closterium extract solution preparation process a is by the material composition of following weight, nitre
Sour 50 milligrams of sodium, 5 milligrams of potassium dihydrogen phosphate, 0.1 milligram of ferric sulfate, 10 milligrams of sodium citrate, 10 milligrams of sodium metasilicate, vitamin
1000 grams of B120.2 micrograms, seawater.
Aspergillus niger in Closterium extract solution preparation process c is purchased from Chinese industrial Microbiological Culture Collection administrative center, protects
Tibetan number：CICC40273；In the step d bacillus amyloliquefaciens conciliate curdled milk series bacillus to be purchased from Chinese industrial micro-
Biological inoculum preservation administrative center, preserving number are respectively：CICC10160、CICC10358.
Embodiment 1：
By 5 grams of sodium nitrate, 0.5 gram of potassium dihydrogen phosphate, 10 milligrams of ferric sulfate, 1 gram of sodium citrate, 1 gram of sodium metasilicate, the dimension life of 20 micrograms
Plain B12 is dissolved in the seawater of double centner, and fermented and cultured is after 92 hours in illumination reaction device for 5 kilograms of Closteriums of access, frond
Quantity reaches maximum；By frond ferment liquid pump people's high pressure homogenizer in, control pressure homogenized 15 minutes under the conditions of 70MPa
After be pumped into fermentation tank；Add 3 kilograms of sucrose, 0.3 kilogram of sodium nitrate, 0.05 kilogram of magnesium sulfate, 0.05 kilogram of potassium chloride and 1
Kilogram dipotassium hydrogen phosphate stirring and dissolving, 2 kilograms of aspergillus niger bacterium solutions are accessed, after being fermented 24 hours at 28 DEG C, temperature is promoted to
121 DEG C insulation 20 minutes after put tank natural cooling；Liquid is pumped into fermentation tank again, the solution starch gemma bar of 1.5 kilograms of access
Bacterium and 2 kilograms of solution curdled milk series bacillus, after being fermented 18 hours at 32 DEG C, fermentation jar temperature are improved to 121 DEG C, insulation 20
Tank, natural cooling are put after minute；After liquid is by 4 layers of filtered through gauze, with low speed centrifuge, 5000 revs/min centrifuge 25 minutes,
Take supernatant；Liquid carries out low pressure rotary evaporation in Rotary Evaporators, volume-diminished to original volume 5% after stop evaporation, it is cold
But 5 kilograms of final Closterium extract solution is obtained afterwards.
Weigh 0.1 kilogram of sodium benzoate, 1 kilogram of sodium alginate and 3 kilograms of carboxymethyl cellulose and be dissolved in 65
Kilogram deionized water in, be sufficiently stirred dissolving it is standby；13 kilograms of polyvinyl alcohol is added in 9 kilograms of ethanol after soaking and added
Enter into above-mentioned solution, be heated to 70 DEG C, be stirred continuously overnight dissolving；Again thereto add 1 kilogram of propane diols, 3 kilograms of glycerine,
1 kilogram of silicon breast and 4 kilograms of Closterium extract solutions and 0.1 kilogram of essence, are sufficiently stirred to obtain Closterium facial treatment mask liquid 100,000
Gram；It is put into after non-woven fabrics is folded in aluminium foil bag, is sealed after 40 milliliters of Closterium facial mask liquids of every bag of addition, obtain Closterium skin care
2500 pieces of facial mask.
Embodiment 2：
By 15 grams of sodium nitrate, 1.5 grams of potassium dihydrogen phosphates, 30 milligrams of ferric sulfate, 3 grams of sodium citrates, 3 grams of sodium metasilicate, 60 micrograms dimension
Raw plain B12 is dissolved in 300 kilograms of seawater, 15 kilograms of Closteriums of access fermented and cultured after 100 hours in illumination reaction device,
Frond quantity reaches maximum；By frond ferment liquid pump people's high pressure homogenizer in, control pressure homogenized 15 under the conditions of 70MPa
It is pumped into after minute in fermentation tank；Add 9 kilograms of sucrose, 0.9 kilogram of sodium nitrate, 0.15 kilogram of magnesium sulfate, 0.15 kilogram of potassium chloride
And 3 kilograms of dipotassium hydrogen phosphate stirring and dissolvings, 6 kilograms of aspergillus niger bacterium solutions are accessed, after being fermented 30 hours at 28 DEG C, temperature is carried
Rise to 121 DEG C insulation 20 minutes after put tank natural cooling；Liquid is pumped into fermentation tank again, the solution starch bud of 4.5 kilograms of access
Spore bacillus and 6 kilograms of solution curdled milk series bacillus, after being fermented 20 hours at 32 DEG C, fermentation jar temperature are improved to 121 DEG C, is protected
Temperature puts tank, natural cooling after 20 minutes；After liquid is by 4 layers of filtered through gauze, with low speed centrifuge, 5000 revs/min centrifuge 25 points
Clock, take supernatant；Liquid carries out low pressure rotary evaporation in Rotary Evaporators, volume-diminished to original volume 10% after stop steaming
Hair, obtains 32 kilograms of final Closterium extract solution after cooling.
0.8 kilogram of sodium benzoate, 8 kilograms of sodium alginate and 24 kilograms of carboxymethyl cellulose is weighed to be dissolved in
In 520 kilograms of deionized water, it is standby to be sufficiently stirred dissolving；104 kilograms of polyvinyl alcohol is added in 72 kilograms of ethanol and moistened
It is added to after wet in above-mentioned solution, is heated to 70 DEG C, is stirred continuously overnight dissolving；Add 8 kilograms of propane diols, 24,000 thereto again
Gram glycerine, 8 kilograms of silicon breasts and 32 kilograms of Closterium extract solutions and 0.8 kilogram of essence, are sufficiently stirred to obtain Closterium skin care face
800 kilograms of film liquid；It is put into after non-woven fabrics is folded in aluminium foil bag, seals, obtain after 50 milliliters of Closterium facial mask liquids of every bag of addition
16000 pieces of Closterium facial treatment mask.
Experimental example 1：
External minimum inhibitory concentration MIC experiments：
Test strain：Fungi, Candida albicans, staphylococcus aureus, enteropathogenic E. Coli.
Method：Bacteria suspension is made with physiological saline in each test strain, and it is more than 2.0McF bacteria suspensions to be diluted to turbidity,
Again with after normal saline dilution, RPMIi640 nutrient solutions dilute, test group（Facial mask liquid in the embodiment of the present invention）And control group
（Common facial mask liquid）Doubling dilution, cultivate 80 hours in 96 well culture plates, slight concussion, 490nm places progress photoelectric colorimetry,
As can be seen that the facial mask liquid fungistatic effect of the present invention is obvious from experiment, to most of kind bacteroid in face skin
There is inhibitory action.
Experimental example 2：
Take the facial mask in the embodiment of the present invention 1, embodiment 2 to be tested, select the experimental subjects of 20, them is used successively
After the beauty mask of embodiment 1,2 and common facial mask, survey is carried out.Survey requirement uses everyone of facial mask, right
In the disparity items such as adhesion sense, flexibility and the moisture retention after using in using and the total satisfaction as facial mask, select
The facial mask oneself more liked is selected, it is as a result as shown in the table,
| Classification | Adhere to sense/name | Flexibility/name | Moisture retention/name | Satisfaction/name |
| Embodiment 1 | 18 | 19 | 18 | 18 |
| Embodiment 2 | 17 | 20 | 18 | 18 |
| Common facial mask | 5 | 4 | 7 | 6 |
As seen from the above table, the more common facial mask of Closterium facial treatment mask of the invention has more preferable satisfaction.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (3)
- A kind of 1. Closterium facial treatment mask, it is characterised in that：It is prepared according to percentage by weight by following steps,（1）0.1% sodium benzoate, 1% sodium alginate and 3% carboxymethyl cellulose is weighed to be dissolved in 65% deionized water, It is standby to be sufficiently stirred dissolving；（2）In 13% polyvinyl alcohol step will be added to after the ethanol wet of addition 9%（1）In solution in be heated to 70 DEG C, Constantly it is stirred, is allowed to well mixed, stands overnight；（3）1% propane diols, 3% glycerine, 1% silicon breast, 4% Closterium extract solution and 0.1% essence are added into step（2）Obtain In solution, it is sufficiently stirred dissolving and both obtains Closterium facial treatment mask liquid；（4）It is put into after non-woven fabrics is folded in aluminium foil bag, adds step（3）Closterium facial treatment mask liquid 20-50 milliliters after seal Mouthful, obtain Closterium facial treatment mask；The step（3）In Closterium extract solution be prepared by following steps,(a) Closterium ferments：It will be fermented in 5% Closterium access Closterium fermentation liquid culture medium, by frond quantity Monitoring, frond quantity reaches maximum after 92-100 hours of fermenting；(b) high pressure broken wall：The Closterium zymotic fluid that step a is obtained is pumped into high pressure homogenizer and carries out high-pressure homogenization, control behaviour Make pressure 70MPa, homogenized natural cooling after 15 minutes；(c) breaking wall by fermentation：In Closterium fermentation liquid pump human hair fermentation tank after broken wall is cooled down, addition mass percent 3% sucrose, After 0.3% sodium nitrate, 0.05% magnesium sulfate, 0.05% potassium chloride and 1% dipotassium hydrogen phosphate stirring and dissolving, 2% black-koji mould is accessed Liquid, after 24-30 hours of being fermented at 28 DEG C, fermentation jar temperature is promoted to 121 DEG C, insulation puts tank, natural cooling after 20 minutes；(d) working substance decomposing and fermenting：Liquid after being cooled down in step c is pumped into fermentation tank again, is respectively connected to 1.5% solution Bacillus amyloliquefacienses and 2% solution curdled milk series bacillus, fermented at 32 DEG C after 18-20 hours, improve fermentation jar temperature extremely 121 DEG C, insulation puts tank, natural cooling after 20 minutes；(e) post-process：After liquid after step d is cooled down is by 4 layers of filtered through gauze, 5000 revs/min of low speed centrifuge is used After centrifugation 25 minutes, take supernatant standby；（f）Extract solution concentrates：The liquid obtained in step e is subjected to low pressure rotary evaporation, volume-diminished in Rotary Evaporators Stop evaporation after to the 5%-10% of original volume, final Closterium extract solution is obtained after cooling.
- A kind of 2. Closterium facial treatment mask according to claim 1, it is characterised in that：The Closterium extract solution prepares step Closterium fermentation liquid culture medium in rapid a be by the material composition of following weight, 50 milligrams of sodium nitrate, 5 milligrams of potassium dihydrogen phosphate, 0.1 milligram of ferric sulfate, 10 milligrams of sodium citrate, 10 milligrams of sodium metasilicate, the microgram of vitamin B12 0.2,1000 grams of seawater.
- A kind of 3. Closterium facial treatment mask according to claim 1, it is characterised in that：The Closterium extract solution prepares step Aspergillus niger in rapid c is purchased from Chinese industrial Microbiological Culture Collection administrative center, preserving number：CICC40273；In the step d Obtain bacillus amyloliquefaciens reconciliation curdled milk series bacillus and be purchased from Chinese industrial Microbiological Culture Collection administrative center, preserving number Respectively：CICC10160、CICC10358.
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| CN201710782780.3A CN107496219A (en) | 2017-09-03 | 2017-09-03 | A kind of Closterium facial treatment mask |
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| CN201710782780.3A CN107496219A (en) | 2017-09-03 | 2017-09-03 | A kind of Closterium facial treatment mask |
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| CN201710782780.3A Pending CN107496219A (en) | 2017-09-03 | 2017-09-03 | A kind of Closterium facial treatment mask |
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| CN102670420A (en) * | 2011-03-18 | 2012-09-19 | 于鹏 | Negative ion mask |
| CN102511847A (en) * | 2012-01-05 | 2012-06-27 | 中国人民解放军第三军医大学第一附属医院 | Preparation method of spirulina extract |
| CN104292939A (en) * | 2014-07-14 | 2015-01-21 | Pac生命科技研发有限公司 | Alga extraction liquid capable of dispersing large-cluster molecules and preparation method thereof |
| CN106606446A (en) * | 2016-12-01 | 2017-05-03 | 方孝贤 | Enzyme sleeping mask with antioxidation function and preparation method thereof |
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