Polypeptide herbal solid beverage with antioxidant function
The technical field is as follows:
the invention relates to a formula of a solid beverage, in particular to a polypeptide herbal solid beverage with an antioxidant function.
Background art:
the life activities are free radicals, the human body activities and the internal activities need to burn energy, and the transporters responsible for transferring the energy are the free radicals. Modern medicine considers that while a series of oxidative metabolism is carried out, the body continuously generates free radicals, the excessive free radicals can act on lipid substances such as blood, cells, tissues and the like to change the lipid peroxides into lipid peroxides, the peroxides are deposited on cell membranes to lose functions of the cell membranes, so that the cell activity is reduced, the functions of tissues and organs are reduced, the body enters an aging state, and various diseases can be induced, such as common diseases such as cancers, arteriosclerosis, diabetes, cataract, cardiovascular diseases, senile dementia, arthritis and the like. Research has shown that lipid peroxidation caused by free radicals plays an important role in the process of histopathological injury, and the degree of cell injury can be regulated through an antioxidant mechanism. The antioxidant substances are derived from the human body itself or are supplied from food, but the amount of antioxidant substances synthesized by the human body itself is limited. Therefore, the proper supplement of the antioxidant has important significance for improving the oxidation resistance of the organism, improving the health level, reducing the disease risk and delaying the aging.
The traditional Chinese medicine has unique understanding on the aging process, and is mostly anti-aging health preserving health care medicines for prolonging life and not aging in the medicines listed as the superior medicines in the Shen nong Ben Cao Jing, for example, ginseng, glossy privet fruit, cistanche, ophiopogon root, medlar and the like are all carried with the effects of losing weight, prolonging life, not hungry and not aging after being taken for a long time. Although a large number of antioxidant solid beverage products exist at present, the antioxidant solid beverage products contain single, rare and high-cost components with antioxidant functions. Therefore, it is urgently needed to provide an antioxidant solid beverage which can reduce raw material cost and improve antioxidant effect, so that the antioxidant solid beverage is widely popularized and applied.
The invention content is as follows:
the invention aims to provide a polypeptide herbal solid beverage which can enhance the antioxidant function of organisms, improve the health level, is safe and has no toxic or side effect and has the antioxidant function.
The invention is implemented by the following technical scheme: the polypeptide herbal solid beverage with the antioxidant function comprises the following components in parts by weight: 1.5-2.0 parts of bone marrow peptide powder, 1.5-2.0 parts of collagen peptide powder, 1.0-3.0 parts of angelica powder, 1.0-3.0 parts of turmeric powder, 1.0-3.0 parts of fructus cannabis powder, 0.5-3.0 parts of honeysuckle and 0.5-3.0 parts of safflower powder.
Further, the coating also comprises an auxiliary material, wherein the auxiliary material is any one or a combination of several of the following components in parts by weight: 0-8.5 parts of maltodextrin, 0-1.5 parts of resistant dextrin and 0-0.2 part of thickening agent.
Further, the taste modifier is any one or combination of the following components in parts by weight: 3.0 parts of full cream milk powder, 1.6 parts of banana powder, 0.7 part of wheat flavor powder, 0-3.0 parts of fructose and 0-0.014 part of sucralose.
Further, the thickening agent is konjac gum.
Further, the bone marrow peptide powder is bovine bone marrow peptide powder or sheep bone marrow peptide powder.
Further, the collagen peptide powder is any one of bovine collagen peptide powder, sheep collagen peptide powder or fish collagen peptide powder; the collagen peptide powder is prepared by a conventional process.
Further, the preparation method of the bovine bone marrow peptide powder or the sheep bone marrow peptide powder comprises the following steps: (1) pretreatment, (2) constant-pressure cooking, (3) enzymolysis, (4) inactivation and sterilization, (5) filtration, (6) concentration and (7) drying; wherein,
(1) pretreatment: putting clean ox bones or sheep bones into a bone crusher, crushing into bone fragments of 3-7 cm, weighing, and putting the weighed bone fragments into a pressure cooking pot;
(2) constant pressure cooking: adding water with the weight 1.0-1.5 times that of the broken bone blocks into the pressure cooking tank, and sealing a feeding port of the pressure cooking tank; the pressure cooking pot is used for cooking the broken bone blocks for 6-8 hours at constant pressure, the pressure during constant pressure cooking is 0.25-0.27 Mpa, the temperature is 127.4-130.0 ℃, and after the constant pressure cooking is finished, cooking liquid is obtained; separating the bone soup in the cooking feed liquid by a centrifugal machine, and conveying the bone soup into an enzymolysis tank for enzymolysis;
(3) enzymolysis: reducing the temperature of the bone soup in the enzymolysis tank to 54-55 ℃, adjusting the pH value of the bone soup to 8.0-8.5 by using a food-grade sodium hydroxide solution with the mass percentage concentration of 40%, adding alkaline protease into the bone soup, uniformly stirring, carrying out enzymolysis at a constant temperature of 54-55 ℃ for 2-3 h, and stirring once every 25-35 min to obtain an enzymolysis liquid I; the addition amount of the alkaline protease is as follows: adding alkaline protease (kg) ═ 0.5-0.8% x bone soup theoretical dry matter (kg);
then, reducing the temperature of the enzymolysis liquid I to 47-49 ℃, adjusting the pH value of the enzymolysis liquid I to 7.5-7.8 by using a sodium hydroxide solution with the mass percentage concentration of 20-40%, adding trypsin into the enzymolysis liquid I, uniformly stirring, carrying out enzymolysis at the constant temperature of 47-49 ℃ for 3-4 h, and stirring once every 25-35 min to obtain an enzymolysis liquid II; the addition amount of the trypsin is as follows: adding trypsin (kg) ═ 0.05-0.2% x theoretical dry matter of the bone soup (kg);
(4) inactivation and sterilization: after the enzymolysis liquid II is obtained, boiling the enzymolysis liquid II for 20-30 min; then, reducing the temperature of the enzymolysis solution II to 80-85 ℃, standing for 2 hours to layer the enzymolysis solution II, wherein the lower layer clear and transparent liquid after layering of the enzymolysis solution II is bone marrow peptide liquid;
(5) and (3) filtering: after the bone marrow peptide liquid is obtained, filtering the bone marrow peptide liquid by using a vacuum filter, wherein the vacuum degree of the vacuum filter is 0.4-0.7 Mpa, the filtering precision is 15-20 mu m, and conveying the filtered bone marrow peptide liquid to a filtrate storage tank for temporary storage;
(6) concentration: adjusting the temperature of the bone marrow peptide liquid stored in the filtrate storage tank to 70-80 ℃, conveying the bone marrow peptide liquid to a vacuum concentrator, carrying out vacuum concentration under the condition that the vacuum degree is 0.06-0.08 Mpa, and finishing the vacuum concentration when the refractive index of the bone marrow peptide liquid is 40-50% to obtain a bone marrow peptide concentrated liquid, and conveying the bone marrow peptide concentrated liquid to a concentrated liquid storage tank for temporary storage;
(7) and (3) drying: adjusting the temperature of the marrow peptide concentrated solution stored in the concentrated solution storage tank to 45-50 ℃, conveying the marrow peptide concentrated solution into a spray drying tower for drying treatment, wherein the inlet temperature of the spray drying tower is 150-160 ℃, the outlet temperature of the spray drying tower is 80-90 ℃, and after the drying treatment is finished, obtaining the bovine marrow peptide powder or the sheep marrow peptide powder.
Further, in the constant-pressure cooking in the step (2), the cooking feed liquid is separated into the bone soup, the grease and the bone blocks through a centrifugal machine, the bone soup is conveyed to the enzymolysis tank for enzymolysis, and the grease is conveyed to the grease tank for treatment as a byproduct; discharging the bone blocks from the pressure cooking pot, drying and crushing the bone blocks into bone powder.
The invention has the advantages that: 1. the bone marrow peptide powder, the collagen powder and the herbal food material are used as main raw materials, so that the bone marrow peptide powder has the effects of enhancing the antioxidant function of an organism and improving the health level, has obvious effect and has better development and market application prospects; 2. the bone marrow peptide powder and the collagen powder are combined with the herbal food material, the theory of homology of medicine and food in traditional Chinese medicine is met, and the product is safe and has no toxic or side effect.
The specific implementation mode is as follows:
example 1:
the formula of the polypeptide herbal solid beverage with the antioxidant function comprises the following components in parts by weight: 1.5 parts of bone marrow peptide powder, 1.5 parts of collagen peptide powder, 1.0 part of angelica powder, 1.0 part of turmeric powder, 1.0 part of fructus cannabis powder, 0.5 part of honeysuckle and 0.5 part of safflower powder. Wherein the bone marrow peptide powder is bovine bone marrow peptide powder, and the collagen peptide powder is bovine collagen peptide powder.
Among the above components, the bone marrow peptide powder has the effects of improving immunity, scavenging free radicals and resisting oxidation; the collagen powder has the effects of improving immunity, supplementing collagen and improving skin moisture; the angelica powder has the effects of enriching blood and activating blood, regulating menstruation and relieving pain, and relaxing bowel, and is used for treating blood deficiency and chlorosis, dizziness and palpitation, irregular menstruation, amenorrhea and dysmenorrheal, deficiency-cold abdominal pain, rheumatic arthralgia, traumatic injury, carbuncle, ulcer and ulcer, and constipation due to intestinal dryness; the turmeric powder has effects of removing blood stasis, activating qi-flowing, dredging channels and relieving pain, and can be used for treating chest and hypochondrium stabbing pain, thoracic obstruction, cardialgia, dysmenorrhea amenorrhea, abdominal mass, rheumatism, shoulder and arm pain, and traumatic injury with swelling and pain; fructus Cannabis has effects of loosening bowel to relieve constipation, and can be used for treating constipation due to blood deficiency and body fluid deficiency; the honeysuckle has the effects of clearing away heat and toxic materials and dispelling wind and heat, and is used for treating carbuncle, furuncle, pharyngitis, erysipelas, heat toxin and bloody dysentery, wind-heat type common cold, epidemic febrile disease and fever; the safflower powder has the effects of activating blood circulation, stimulating menstrual flow, dissipating blood stasis and relieving pain.
The production process of the embodiment comprises the following steps: (1) preparing bovine bone marrow peptide powder, (2) preparing bovine collagen peptide powder, (3) weighing the components, (4) mixing the materials, (5) packaging and warehousing, and (6) inspecting; wherein,
(1) preparing bovine bone marrow peptide powder: which comprises the following steps: 1.1 pretreatment, 1.2 constant pressure cooking, 1.3 enzymolysis, 1.4 inactivation and sterilization, 1.5 filtration, 1.6 concentration and 1.7 drying; wherein,
1.1 pretreatment: putting clean ox bones into a bone crusher, crushing into bone fragments of 3cm, weighing to obtain 3000kg of ox bones, and putting the weighed bone fragments into a pressure cooking pot;
1.2 constant pressure cooking: adding 3000kg of water into the pressure cooking pot, wherein the weight of the water is 1.0 time of that of the broken bone blocks, and sealing a feeding port of the pressure cooking pot; using a pressure cooking pot to cook the broken bone blocks for 6 hours at constant pressure, wherein the pressure during constant pressure cooking is 0.25Mpa, the temperature is 127.4 ℃, and after the constant pressure cooking is finished, cooking liquid is obtained; separating the cooking feed liquid into bone soup, grease and bone blocks through a centrifugal machine, conveying the bone soup to an enzymolysis tank for enzymolysis treatment, and conveying the grease to the grease tank for treatment as a byproduct; discharging the bone blocks from the pressure cooking pot, drying, and pulverizing into bone powder;
1.3, enzymolysis: reducing the temperature of the bone soup in the enzymolysis tank to 54 ℃, adjusting the pH value of the bone soup to 8.0 by using food-grade sodium hydroxide solution with the mass percentage concentration of 40%, adding alkaline protease into the bone soup, uniformly stirring, carrying out enzymolysis at the constant temperature of 54 ℃ for 2 hours, and stirring once every 25min to obtain an enzymolysis liquid I; the addition amount of the alkaline protease is as follows: the amount of alkaline protease added (kg) is 0.5% × bone soup theoretical dry matter (kg) is 0.5% × 330.4% × 1.652 (kg);
then, reducing the temperature of the enzymolysis liquid I to 47 ℃, adjusting the pH value of the enzymolysis liquid I to 7.5 by using a sodium hydroxide solution with the mass percentage concentration of 20%, adding trypsin into the enzymolysis liquid I, uniformly stirring, carrying out enzymolysis for 3 hours at the constant temperature of 47 ℃, and stirring once every 25 minutes to obtain an enzymolysis liquid II; the addition amount of trypsin is as follows: trypsin is added in an amount (kg) of 0.05% × bone soup theoretical dry matter (kg) of 0.05% × 330.4% × 0.17 (kg);
the theoretical dry matter of the bone soup is calculated by the formula: theory of bone soup dry matter (kg) ═ bone soup refractive index% x bone soup volume (m)3) X specific gravity (kg/m)3)=4.9%×2593m3×0.026(kg/m3)=330.4(kg);
1.4 inactivation and sterilization: after obtaining the enzymolysis liquid II, boiling the enzymolysis liquid II for 20 min; then, reducing the temperature of the enzymolysis solution II to 80 ℃, standing for 2 hours to enable the enzymolysis solution II to be layered, wherein the lower layer clear and transparent liquid after layering of the enzymolysis solution II is bone marrow peptide liquid;
1.5, filtering: after obtaining the bone marrow peptide liquid, filtering the bone marrow peptide liquid by using a vacuum filter, wherein the vacuum degree of the vacuum filter is 0.4Mpa, the filtering precision is 15 mu m, and conveying the filtered bone marrow peptide liquid into a filtrate storage tank for temporary storage;
1.6, concentrating: adjusting the temperature of the bone marrow peptide liquid stored in the filtrate storage tank to 70 ℃, conveying the bone marrow peptide liquid to a vacuum concentrator, carrying out vacuum concentration under the condition that the vacuum degree is 0.06Mpa until the refractive index of the bone marrow peptide liquid is 40%, finishing the vacuum concentration, obtaining the bone marrow peptide concentrated liquid, and conveying the bone marrow peptide concentrated liquid to a concentrated liquid storage tank for temporary storage;
1.7, drying: regulating the temperature of the marrow peptide concentrated solution stored in the concentrated solution storage tank to 45 ℃, conveying the marrow peptide concentrated solution into a spray drying tower for drying treatment, wherein the inlet temperature of the spray drying tower is 150 ℃, the outlet temperature of the spray drying tower is 80 ℃, and obtaining the bovine marrow peptide powder after the drying treatment is finished.
(2) Preparing bovine collagen peptide powder: cleaning and crushing ox bones, placing the crushed ox bones in a cooking device, adding water with the mass of 1 time of the ox bones, boiling for 15min, discharging soup, adding water with the mass of 1.2 times of the ox bones into the cooking device, and cooking to obtain collagen liquid; then, adding the collagen liquid and the enzyme which is activated for 20min into an enzymolysis device for enzymolysis; filtering after enzymolysis is finished, and concentrating clear and light yellow filtrate to obtain concentrated filtrate; and (4) concentrating the filtrate, carrying out spray drying, and sieving a dried crude product with a 80-mesh sieve to obtain the bovine collagen peptide powder.
(3) Weighing the following components: weighing and mixing the following components in parts by weight: 1.5 parts of bovine bone marrow peptide powder, 1.5 parts of bovine collagen peptide powder, 1.0 part of angelica powder, 1.0 part of turmeric powder, 1.0 part of fructus cannabis powder, 0.5 part of honeysuckle and 0.5 part of safflower powder.
(4) Mixing materials: and (3) placing the weighed and mixed components into a three-dimensional mixer, starting the three-dimensional mixer, and fully and uniformly mixing the components at the mixing speed of 2 revolutions per minute for 30min to obtain a semi-finished product material.
(5) Packaging and warehousing: packaging the uniformly mixed semi-finished product materials through full-automatic powder packaging to obtain a finished product; and (5) boxing, code spraying, plastic packaging, boxing and warehousing the finished product.
(6) And (4) checking: the finished product was tested according to GB/T29602 solid beverage.
Example 2:
the formula of the polypeptide herbal solid beverage with the antioxidant function comprises the following components in parts by weight: 1.7 parts of bone marrow peptide powder, 1.7 parts of collagen peptide powder, 1.2 parts of angelica powder, 1.2 parts of turmeric powder, 1.2 parts of fructus cannabis powder, 0.7 part of honeysuckle and 0.7 part of safflower powder. Wherein the bone marrow peptide powder is bovine bone marrow peptide powder, and the collagen peptide powder is sheep collagen peptide powder.
The adhesive also comprises auxiliary materials, wherein the auxiliary materials comprise the following components in parts by weight: 0.5 part of resistant dextrin and 0.05 part of thickening agent, wherein the thickening agent in the embodiment is konjac gum. The taste conditioning agent comprises the following components in parts by weight: 1.0 part of full cream milk powder.
Among the above components, the bone marrow peptide powder has the effects of improving immunity, scavenging free radicals and resisting oxidation; the collagen powder has the effects of improving immunity, supplementing collagen and improving skin moisture; the angelica powder has the effects of enriching blood and activating blood, regulating menstruation and relieving pain, and relaxing bowel, and is used for treating blood deficiency and chlorosis, dizziness and palpitation, irregular menstruation, amenorrhea and dysmenorrheal, deficiency-cold abdominal pain, rheumatic arthralgia, traumatic injury, carbuncle, ulcer and ulcer, and constipation due to intestinal dryness; the turmeric powder has effects of removing blood stasis, activating qi-flowing, dredging channels and relieving pain, and can be used for treating chest and hypochondrium stabbing pain, thoracic obstruction, cardialgia, dysmenorrhea amenorrhea, abdominal mass, rheumatism, shoulder and arm pain, and traumatic injury with swelling and pain; fructus Cannabis has effects of loosening bowel to relieve constipation, and can be used for treating constipation due to blood deficiency and body fluid deficiency; the honeysuckle has the effects of clearing away heat and toxic materials and dispelling wind and heat, and is used for treating carbuncle, furuncle, pharyngitis, erysipelas, heat toxin and bloody dysentery, wind-heat type common cold, epidemic febrile disease and fever; the safflower powder has the effects of activating blood circulation, stimulating menstrual flow, dissipating blood stasis and relieving pain. The resistant dextrin can increase the solubility and ensure that the product has better reconstitution property; the thickening agent can adjust the viscosity; the whole milk powder can regulate taste.
The production process of the embodiment comprises the following steps: (1) preparing bovine bone marrow peptide powder, (2) preparing sheep collagen peptide powder, (3) weighing the components, (4) mixing the materials, (5) packaging and warehousing, and (6) inspecting; wherein,
(1) preparing bovine bone marrow peptide powder: which comprises the following steps: 1.1 pretreatment, 1.2 constant pressure cooking, 1.3 enzymolysis, 1.4 inactivation and sterilization, 1.5 filtration, 1.6 concentration and 1.7 drying; wherein,
1.1 pretreatment: putting clean ox bones into a bone crusher, crushing into bone fragments with the length of 3cm, weighing, and putting the weighed bone fragments into a pressure cooking pot, wherein the mass of the bone fragments is 2600 kg;
1.2 constant pressure cooking: adding water which is 1.0 time of the weight of the broken bone blocks into the pressure cooking pot, namely adding 2600kg of water, and sealing a feeding port of the pressure cooking pot; using a pressure cooking pot to cook the broken bone blocks for 6 hours at constant pressure, wherein the pressure during constant pressure cooking is 0.25Mpa, the temperature is 127.4 ℃, and after the constant pressure cooking is finished, cooking liquid is obtained; separating the cooking feed liquid into bone soup, grease and bone blocks through a centrifugal machine, conveying the bone soup to an enzymolysis tank for enzymolysis treatment, and conveying the grease to the grease tank for treatment as a byproduct; discharging the bone blocks from the pressure cooking pot, drying, and pulverizing into bone powder;
1.3, enzymolysis: reducing the temperature of the bone soup in the enzymolysis tank to 54 ℃, adjusting the pH value of the bone soup to 8.0 by using food-grade sodium hydroxide solution with the mass percentage concentration of 40%, adding alkaline protease into the bone soup, uniformly stirring, carrying out enzymolysis at the constant temperature of 54 ℃ for 2 hours, and stirring once every 25min to obtain an enzymolysis liquid I; the addition amount of the alkaline protease is as follows: the amount of alkaline protease added (kg) is 0.5% × bone soup theoretical dry matter (kg) is 0.5% × 369.6(kg) is 1.85 (kg);
then, reducing the temperature of the enzymolysis liquid I to 47 ℃, adjusting the pH value of the enzymolysis liquid I to 7.5 by using a sodium hydroxide solution with the mass percentage concentration of 20%, adding trypsin into the enzymolysis liquid I, uniformly stirring, carrying out enzymolysis for 3-4 h at the constant temperature of 47 ℃, and stirring once every 25min to obtain an enzymolysis liquid II; the addition amount of trypsin is as follows: trypsin is added in an amount (kg) of 0.05% × bone soup theoretical dry matter (kg) of 0.05% × 369.6(kg) of 0.185 (kg);
the theoretical dry matter of the bone soup is calculated by the formula: bone soup theory dry matter (kg): refractive index%X volume of bone soup (m)3) X specific gravity (kg/m)3)=5.3%×2682(m3)×0.026(kg/m3)=369.6(kg);
1.4 inactivation and sterilization: after obtaining the enzymolysis liquid II, boiling the enzymolysis liquid II for 20 min; then, reducing the temperature of the enzymolysis solution II to 80 ℃, standing for 2 hours to enable the enzymolysis solution II to be layered, wherein the lower layer clear and transparent liquid after layering of the enzymolysis solution II is bone marrow peptide liquid;
1.5, filtering: after obtaining the bone marrow peptide liquid, filtering the bone marrow peptide liquid by using a vacuum filter, wherein the vacuum degree of the vacuum filter is 0.4Mpa, the filtering precision is 15 mu m, and conveying the filtered bone marrow peptide liquid into a filtrate storage tank for temporary storage;
1.6, concentrating: adjusting the temperature of the bone marrow peptide liquid stored in the filtrate storage tank to 70 ℃, conveying the bone marrow peptide liquid to a vacuum concentrator, carrying out vacuum concentration under the condition that the vacuum degree is 0.06Mpa until the refractive index of the bone marrow peptide liquid is 40%, finishing the vacuum concentration, obtaining the bone marrow peptide concentrated liquid, and conveying the bone marrow peptide concentrated liquid to a concentrated liquid storage tank for temporary storage;
1.7, drying: regulating the temperature of the marrow peptide concentrated solution stored in the concentrated solution storage tank to 45 ℃, conveying the marrow peptide concentrated solution into a spray drying tower for drying treatment, wherein the inlet temperature of the spray drying tower is 150 ℃, the outlet temperature of the spray drying tower is 80 ℃, and obtaining the bovine marrow peptide powder after the drying treatment is finished.
(2) Preparing sheep collagen peptide powder: removing hair and hoof pods of the rumex japonicus, cleaning, putting the cleaned rumex japonicus into a cooking device, adding water with the mass of 1 time of the rumex japonicus, and cooking to obtain collagen liquid; then, adding the collagen liquid and the enzyme which is activated for 20min into an enzymolysis device for enzymolysis; filtering after enzymolysis is finished, and concentrating clear and light yellow filtrate to obtain concentrated filtrate; and (4) concentrating the filtrate, carrying out spray drying, and sieving a dried crude product with a 80-mesh sieve to obtain the bovine collagen peptide powder.
(3) Weighing the following components: weighing and mixing the following components in parts by weight: 1.7 parts of bovine bone marrow peptide powder, 1.7 parts of sheep collagen peptide powder, 1.2 parts of Chinese angelica powder, 1.2 parts of turmeric powder, 1.2 parts of fructus cannabis powder, 0.7 part of honeysuckle and 0.7 part of safflower powder; then weighing and mixing the following auxiliary materials and taste conditioning agents in parts by weight: the auxiliary materials comprise the following components in parts by weight: 0.5 part of resistant dextrin and 0.05 part of thickening agent, wherein the thickening agent in the embodiment is konjac gum; the taste conditioning agent comprises the following components in parts by weight: 1.0 part of full cream milk powder.
(4) Mixing materials: and (3) placing the weighed and mixed components and auxiliary materials into a three-dimensional mixer, starting the three-dimensional mixer, and mixing at the speed of 2 revolutions per minute for 30min to fully and uniformly mix the components and the auxiliary materials to obtain a semi-finished product material.
(5) Packaging and warehousing: packaging the uniformly mixed semi-finished product materials through full-automatic powder packaging to obtain a finished product; and (5) boxing, code spraying, plastic packaging, boxing and warehousing the finished product.
(6) And (4) checking: the finished product was tested according to GB/T29602 solid beverage.
Example 3:
the formula of the polypeptide herbal solid beverage with the antioxidant function comprises the following components in parts by weight: 1.8 parts of bone marrow peptide powder, 1.8 parts of collagen peptide powder, 1.5 parts of angelica powder, 1.2 parts of turmeric powder, 1.2 parts of fructus cannabis powder, 1.0 part of honeysuckle and 1.0 part of safflower powder. Wherein the bone marrow peptide powder is sheep bone marrow peptide powder, and the collagen peptide powder is fish collagen peptide powder.
The adhesive also comprises auxiliary materials, wherein the auxiliary materials comprise the following components in parts by weight: 2.0 parts of maltodextrin, 1.0 part of resistant dextrin and 1.0 part of thickening agent, wherein the thickening agent in the embodiment is colloid. The taste conditioning agent comprises the following components in parts by weight: 2.0 parts of full cream milk powder, 1.6 parts of banana powder and 1.5 parts of fructose.
Among the above components, the bone marrow peptide powder has the effects of improving immunity, scavenging free radicals and resisting oxidation; the collagen powder has the effects of improving immunity, supplementing collagen and improving skin moisture; the angelica powder has the effects of enriching blood and activating blood, regulating menstruation and relieving pain, and relaxing bowel, and is used for treating blood deficiency and chlorosis, dizziness and palpitation, irregular menstruation, amenorrhea and dysmenorrheal, deficiency-cold abdominal pain, rheumatic arthralgia, traumatic injury, carbuncle, ulcer and ulcer, and constipation due to intestinal dryness; the turmeric powder has effects of removing blood stasis, activating qi-flowing, dredging channels and relieving pain, and can be used for treating chest and hypochondrium stabbing pain, thoracic obstruction, cardialgia, dysmenorrhea amenorrhea, abdominal mass, rheumatism, shoulder and arm pain, and traumatic injury with swelling and pain; fructus Cannabis has effects of loosening bowel to relieve constipation, and can be used for treating constipation due to blood deficiency and body fluid deficiency; the honeysuckle has the effects of clearing away heat and toxic materials and dispelling wind and heat, and is used for treating carbuncle, furuncle, pharyngitis, erysipelas, heat toxin and bloody dysentery, wind-heat type common cold, epidemic febrile disease and fever; the safflower powder has the effects of activating blood circulation, stimulating menstrual flow, dissipating blood stasis and relieving pain. The resistant dextrin can increase the solubility and ensure that the product has better reconstitution property; the thickening agent can adjust the viscosity; the fructose can regulate the sweetness; maltodextrin, whole milk powder, and banana powder can regulate taste.
The production process of the embodiment comprises the following steps: (1) preparing sheep bone marrow peptide powder, (2) preparing fish collagen peptide powder, (3) weighing the components, (4) mixing the materials, (5) packaging and warehousing, and (6) inspecting; wherein,
(1) preparing sheep bone marrow peptide powder: which comprises the following steps: 1.1 pretreatment, 1.2 constant pressure cooking, 1.3 enzymolysis, 1.4 inactivation and sterilization, 1.5 filtration, 1.6 concentration and 1.7 drying; wherein,
1.1 pretreatment: putting clean sheep bones into a bone crusher, crushing the sheep bones into 5cm bone fragments, weighing the bone fragments to obtain 3000kg of sheep bones, and putting the weighed bone fragments into a pressure cooking pot;
1.2 constant pressure cooking: adding 3900kg of water which is 1.3 times the weight of the broken bone blocks into the pressure cooking pot, and sealing a feeding port of the pressure cooking pot; using a pressure cooking pot to cook the broken bone blocks for 7 hours at constant pressure, wherein the pressure during constant pressure cooking is 0.26Mpa, the temperature is 128.9 ℃, and after the constant pressure cooking is finished, cooking liquid is obtained; separating the cooking feed liquid into bone soup, grease and bone blocks through a centrifugal machine, conveying the bone soup to an enzymolysis tank for enzymolysis treatment, and conveying the grease to the grease tank for treatment as a byproduct; discharging the bone blocks from the pressure cooking pot, drying, and pulverizing into bone powder;
1.3, enzymolysis: reducing the temperature of the bone soup in the enzymolysis tank to 54.5 ℃, adjusting the pH value of the bone soup to 8.3 by using a food additive sodium hydroxide solution with the mass percentage concentration of 40%, adding alkaline protease into the bone soup, uniformly stirring, carrying out enzymolysis at the constant temperature of 54.5 ℃ for 2.5h, and stirring once every 30min to obtain an enzymolysis liquid I; the addition amount of the alkaline protease is as follows: the amount of alkaline protease added (kg) was 0.7% × bone soup theoretical dry matter (kg) was 0.7% × 472(kg) was 3.31 (kg);
then, reducing the temperature of the enzymolysis solution I to 48 ℃, adjusting the pH value of the enzymolysis solution I to 7.7 by using a sodium hydroxide solution with the mass percentage concentration of 30%, adding trypsin into the enzymolysis solution I, uniformly stirring, performing enzymolysis at constant temperature for 3.5h, and stirring once every 30min to obtain an enzymolysis solution II; the addition amount of trypsin is as follows: trypsin is added in an amount (kg) of 0.15% × bone soup theoretical dry matter (kg) of 0.15% × 472% (kg) of 0.71 (kg);
the theoretical dry matter of the bone soup is calculated by the formula: theory of bone soup dry matter (kg) ═ bone soup refractive index% x bone soup volume (m)3) X specific gravity (kg/m)3)=6.6%×2753(m3)×0.026(kg/m3)=472(kg);
1.4 inactivation and sterilization: after obtaining the enzymolysis liquid II, boiling the enzymolysis liquid II for 25 min; then, reducing the temperature of the enzymolysis solution II to 83 ℃, standing for 2 hours to enable the enzymolysis solution II to be layered, wherein the lower layer of the layered enzymolysis solution II is clear and transparent liquid which is bone marrow peptide liquid;
1.5, filtering: after obtaining the bone marrow peptide liquid, filtering the bone marrow peptide liquid by using a vacuum filter, wherein the vacuum degree of the vacuum filter is 0.6Mpa, the filtering precision is 18 mu m, and conveying the filtered bone marrow peptide liquid into a filtrate storage tank for temporary storage;
1.6, concentrating: adjusting the temperature of the bone marrow peptide liquid stored in the filtrate storage tank to 75 ℃, conveying the bone marrow peptide liquid to a vacuum concentrator, carrying out vacuum concentration under the condition that the vacuum degree is 0.07Mpa until the refractive index of the bone marrow peptide liquid is 45%, finishing the vacuum concentration to obtain a bone marrow peptide concentrated solution, and conveying the bone marrow peptide concentrated solution to a concentrated solution storage tank for temporary storage;
1.7, drying: adjusting the temperature of the bone marrow peptide concentrated solution stored in the concentrated solution storage tank to 48 ℃, conveying the bone marrow peptide concentrated solution into a spray drying tower for drying treatment, wherein the inlet temperature of the spray drying tower is 155 ℃, the outlet temperature of the spray drying tower is 85 ℃, and obtaining the sheep bone marrow peptide powder after the drying treatment.
(2) Preparing fish collagen peptide powder: cleaning and crushing fish skin, placing the crushed fish skin into cooking equipment, and adding water with the mass 1 time that of the fish skin for cooking to obtain collagen liquid; then, adding the collagen liquid and the enzyme which is activated for 20min into an enzymolysis device for enzymolysis; filtering after enzymolysis is finished, and concentrating clear and light yellow filtrate to obtain concentrated filtrate; and (4) concentrating the filtrate, carrying out spray drying, and sieving a dried crude product with a 80-mesh sieve to obtain the bovine collagen peptide powder.
(3) Weighing the following components: weighing and mixing the following components in parts by weight: 1.8 parts of sheep bone marrow peptide powder, 1.8 parts of fish collagen peptide powder, 1.5 parts of Chinese angelica powder, 1.2 parts of turmeric powder, 1.2 parts of fructus cannabis powder, 1.0 part of honeysuckle and 1.0 part of safflower powder; then weighing and mixing the following auxiliary materials and taste conditioning agents in parts by weight, wherein the auxiliary materials comprise the following components in parts by weight: 2.0 parts of maltodextrin, 1.0 part of resistant dextrin and 1.0 part of thickening agent, wherein the thickening agent in the embodiment is colloid; the taste conditioning agent comprises the following components in parts by weight: 2.0 parts of full cream milk powder, 1.6 parts of banana powder and 1.5 parts of fructose.
(4) Mixing materials: and (3) placing the weighed and mixed components and auxiliary materials into a three-dimensional mixer, starting the three-dimensional mixer, and fully and uniformly mixing the components at the mixing speed of 2 revolutions per minute for 35min to obtain a semi-finished product material.
(5) Packaging and warehousing: packaging the uniformly mixed semi-finished product materials through full-automatic powder packaging to obtain a finished product; and (5) boxing, code spraying, plastic packaging, boxing and warehousing the finished product.
(6) And (4) checking: the finished product was tested according to GB/T29602 solid beverage.
Example 4:
the formula of the polypeptide herbal solid beverage with the antioxidant function comprises the following components in parts by weight: 2.0 parts of bone marrow peptide powder, 2.0 parts of collagen peptide powder, 3.0 parts of angelica powder, 3.0 parts of turmeric powder, 3.0 parts of fructus cannabis powder, 3.0 parts of honeysuckle flower and 3.0 parts of safflower powder. Wherein the bone marrow peptide powder is sheep bone marrow peptide powder, and the collagen peptide powder is sheep collagen peptide powder.
The adhesive also comprises auxiliary materials, wherein the auxiliary materials comprise the following components in parts by weight: 8.5 parts of maltodextrin, 1.5 parts of resistant dextrin and 0.2 part of thickening agent, wherein the thickening agent in the embodiment is 0.1 part of konjac glucomannan and 0.1 part of colloid. The taste conditioning agent comprises the following components in parts by weight: 3.0 parts of full cream milk powder, 4.0 parts of banana powder, 0.7 part of wheat flavor powder, 3.0 parts of fructose and 0.014 part of sucralose.
Among the above components, the bone marrow peptide powder has the effects of improving immunity, scavenging free radicals and resisting oxidation; the collagen powder has the effects of improving immunity, supplementing collagen and improving skin moisture; the angelica powder has the effects of enriching blood and activating blood, regulating menstruation and relieving pain, and relaxing bowel, and is used for treating blood deficiency and chlorosis, dizziness and palpitation, irregular menstruation, amenorrhea and dysmenorrheal, deficiency-cold abdominal pain, rheumatic arthralgia, traumatic injury, carbuncle, ulcer and ulcer, and constipation due to intestinal dryness; the turmeric powder has effects of removing blood stasis, activating qi-flowing, dredging channels and relieving pain, and can be used for treating chest and hypochondrium stabbing pain, thoracic obstruction, cardialgia, dysmenorrhea amenorrhea, abdominal mass, rheumatism, shoulder and arm pain, and traumatic injury with swelling and pain; fructus Cannabis has effects of loosening bowel to relieve constipation, and can be used for treating constipation due to blood deficiency and body fluid deficiency; the honeysuckle has the effects of clearing away heat and toxic materials and dispelling wind and heat, and is used for treating carbuncle, furuncle, pharyngitis, erysipelas, heat toxin and bloody dysentery, wind-heat type common cold, epidemic febrile disease and fever; the safflower powder has the effects of activating blood circulation, stimulating menstrual flow, dissipating blood stasis and relieving pain. The resistant dextrin can increase the solubility and ensure that the product has better reconstitution property; the thickening agent can adjust the viscosity; the fructose and the sucralose can adjust the sweetness; the maltodextrin, whole milk powder, banana powder and wheat flavor powder can regulate taste.
The production process of the embodiment comprises the following steps: (1) preparing sheep bone marrow peptide powder, (2) preparing sheep collagen peptide powder, (3) weighing the components, (4) mixing the materials, (5) packaging and warehousing, and (6) inspecting; wherein,
(1) preparing sheep bone marrow peptide powder: which comprises the following steps: 1.1 pretreatment, 1.2 constant pressure cooking, 1.3 enzymolysis, 1.4 inactivation and sterilization, 1.5 filtration, 1.6 concentration and 1.7 drying; wherein,
1.1 pretreatment: putting clean sheep bones into a bone crusher, crushing the cleaned sheep bones into broken bone blocks of 7cm, weighing the broken bone blocks to obtain 3100kg of sheep bones, and putting the weighed broken bone blocks into a pressure cooking pot;
1.2 constant pressure cooking: adding water with the weight 1.5 times that of the broken bone blocks into the pressure cooking pot, and sealing a feeding port of the pressure cooking pot; using a pressure cooking pot to cook the broken bone blocks for 8 hours at constant pressure, wherein the pressure during constant pressure cooking is 0.27Mpa, the temperature is 130.0 ℃, and after the constant pressure cooking is finished, cooking liquid is obtained; separating the cooking feed liquid into bone soup, grease and bone blocks through a centrifugal machine, conveying the bone soup to an enzymolysis tank for enzymolysis treatment, and conveying the grease to the grease tank for treatment as a byproduct; discharging the bone blocks from the pressure cooking pot, drying, and pulverizing into bone powder;
1.3, enzymolysis: reducing the temperature of the bone soup in the enzymolysis tank to 55 ℃, adjusting the pH value of the bone soup to 8.5 by using food-grade sodium hydroxide solution with the mass percentage concentration of 40%, adding alkaline protease into the bone soup, uniformly stirring, carrying out enzymolysis at the constant temperature of 55 ℃ for 3h, and stirring once every 35min to obtain an enzymolysis liquid I; the addition amount of the alkaline protease is as follows: the amount of alkaline protease added (kg) is 0.8% × bone soup theoretical dry matter (kg) is 0.5% × 409.8(kg) is 2.05 (kg);
then, reducing the temperature of the enzymolysis liquid I to 49 ℃, adjusting the pH value of the enzymolysis liquid I to 7.8 by using a sodium hydroxide solution with the mass percentage concentration of 40%, adding trypsin into the enzymolysis liquid I, uniformly stirring, carrying out enzymolysis for 4 hours at the constant temperature of 49 ℃, and stirring once every 35min to obtain an enzymolysis liquid II; the addition amount of trypsin is as follows: trypsin is added in an amount (kg) of 0.2% × bone soup theoretical dry matter (kg) of 0.2% × 409.8(kg) of 0.82 (kg);
the theoretical dry matter of the bone soup is calculated by the formula: theory of bone soup dry matter (kg) ═ bone soup refractive index% x bone soup volume (m)3) X specific gravity (kg/m)3)=5.8%×2718(m3)×0.026(kg/m3)=409.8(kg);
1.4 inactivation and sterilization: after obtaining the enzymolysis liquid II, boiling the enzymolysis liquid II for 30 min; then, reducing the temperature of the enzymolysis solution II to 85 ℃, standing for 2 hours to layer the enzymolysis solution II, wherein the lower layer clear and transparent liquid after layering the enzymolysis solution II is bone marrow peptide liquid;
1.5, filtering: after obtaining the bone marrow peptide liquid, filtering the bone marrow peptide liquid by using a vacuum filter, wherein the vacuum degree of the vacuum filter is 0.7Mpa, the filtering precision is 20 mu m, and conveying the filtered bone marrow peptide liquid into a filtrate storage tank for temporary storage;
1.6, concentrating: adjusting the temperature of the bone marrow peptide liquid stored in the filtrate storage tank to 80 ℃, conveying the bone marrow peptide liquid to a vacuum concentrator, concentrating the bone marrow peptide liquid in vacuum under the condition that the vacuum degree is 0.08Mpa until the refractive index of the bone marrow peptide liquid is 50%, finishing vacuum concentration to obtain bone marrow peptide concentrated liquid, and conveying the bone marrow peptide concentrated liquid to a concentrated liquid storage tank for temporary storage;
1.7, drying: adjusting the temperature of the bone marrow peptide concentrated solution stored in the concentrated solution storage tank to 50 ℃, conveying the bone marrow peptide concentrated solution into a spray drying tower for drying treatment, wherein the inlet temperature of the spray drying tower is 160 ℃, the outlet temperature of the spray drying tower is 90 ℃, and obtaining the sheep bone marrow peptide powder after the drying treatment.
(2) Preparing sheep collagen peptide powder: removing hair and hoof pods of the rumex japonicus, cleaning, putting the cleaned rumex japonicus into a cooking device, adding water with the mass of 1 time of the rumex japonicus, and cooking to obtain collagen liquid; then, adding the collagen liquid and the enzyme which is activated for 20min into an enzymolysis device for enzymolysis; filtering after enzymolysis is finished, and concentrating clear and light yellow filtrate to obtain concentrated filtrate; and (4) concentrating the filtrate, carrying out spray drying, and sieving a dried crude product with a 80-mesh sieve to obtain the bovine collagen peptide powder.
(3) Weighing the following components: weighing and mixing the following components in parts by weight: 2.0 parts of sheep bone marrow peptide powder, 2.0 parts of sheep collagen peptide powder, 3.0 parts of angelica powder, 3.0 parts of turmeric powder, 3.0 parts of fructus cannabis powder, 3.0 parts of honeysuckle and 3.0 parts of safflower powder; then weighing and mixing the following auxiliary materials and taste conditioning agents in parts by weight, wherein the auxiliary materials comprise the following components in parts by weight: 8.5 parts of maltodextrin, 1.5 parts of resistant dextrin and 0.2 part of thickening agent, wherein the thickening agent in the embodiment is 0.1 part of konjac glucomannan and 0.1 part of colloid; the taste conditioning agent comprises the following components in parts by weight: 3.0 parts of full cream milk powder, 4.0 parts of banana powder, 0.7 part of wheat flavor powder, 3.0 parts of fructose and 0.014 part of sucralose.
(4) Mixing materials: and (3) placing the weighed and mixed components and auxiliary materials into a three-dimensional mixer, starting the three-dimensional mixer, and fully and uniformly mixing the components and the auxiliary materials at the mixing speed of 2 revolutions per minute for 40min to obtain a semi-finished product material.
(5) Packaging and warehousing: packaging the uniformly mixed semi-finished product materials through full-automatic powder packaging to obtain a finished product; and (5) boxing, code spraying, plastic packaging, boxing and warehousing the finished product.
(6) And (4) checking: the finished product was tested according to GB/T29602 solid beverage.
Example 5:
the antioxidant tracking test is carried out by taking the antioxidant tracking test sample as a sample.
Firstly, selecting a subject:
the test subjects are selected from the group who are 45-65 years old, have good physical health condition, have no obvious brain, heart, liver, lung, kidney and blood diseases, have no long-term medicine taking history and are volunteered to ensure the cooperation. The test population excluded the following subjects: women under the age of 45 or over the age of 65, pregnant or lactating; patients with serious diseases of heart, liver, kidney and hematopoietic system; taking articles related to the tested function in a short time affects the judgment of the result.
II, trial design and grouping:
the life and diet of two groups of cases are not interfered, 105 effective cases are randomly selected according to the selection standard of a subject, the effective cases are randomly divided into a test group and a control group according to the levels of MDA (malondialdehyde), SOD (superoxide dismutase) and GSH-Px (glutathione peroxidase) in blood serum of the subject, wherein 52 cases in the test group and 53 cases in the control group are respectively taken with the placebo and the placebo, the placebo is soluble substance which has no influence on the test of the embodiment and has no nutritional value, and the placebo is maltodextrin in the embodiment; the administration is continued for 3 months at a dose of 30 g/d.
The sex and age of the two groups are shown in table 1, the MDA, SOD and GSH-Px levels in the serum of the two groups are shown in table 2, and the living dietary habit of the two groups is shown in table 3.
Table 1 general conditions of the test population
Through statistical test, the gender difference of two groups of subjects is not significant (P > 0.05); age differences were not significant (P > 0.05).
TABLE 2 serum MDA, SOD, GSH-Px levels in two groups of subjects
Through statistical test, the difference of the MDA content in the blood serum of two groups of subjects has no significance (P is more than 0.05); the SOD activity difference in serum has no significance (P is more than 0.05); the difference in the activity of GSH-Px in serum was not significant (P > 0.05).
TABLE 3 dietary habits of two groups of subjects
The mental difference of two groups of subjects is not significant by statistical test (P > 0.05); sleep differences were not significant (P > 0.05); diet differences were not significant (P > 0.05); the difference in stool and urine was not significant (P > 0.05).
Thirdly, observing indexes: each index was measured 1 time at the beginning and end of the test.
1. The safety index is as follows: including general conditions (mental, sleep, diet, stool and urine), routine blood, routine urine, routine feces, chest X-ray, electrocardiogram and B-ultrasound of abdomen; liver function tests [ Total Protein (TP), Albumin (ALB), alanine Aminotransferase (ALT), aspartate Aminotransferase (AST) ]; renal function test [ serum urea nitrogen (BUN), Creatinine (CR) level ] and other test indexes.
2. The efficacy indexes are as follows: changes of lipid peroxide content, superoxide dismutase activity and glutathione peroxidase activity in serum are used as investigation indexes, changes of tested population before and after use are detected, and the antioxidant effect of the product is confirmed.
Fourth, results and analysis
1. And (3) safety index detection results:
before and after the test, the changes of the subjects in the test group and the control group in the aspects of spirit, sleep, diet, defecation, blood pressure and the like are shown in the tables 4 and 5 respectively.
TABLE 4 dietary habits of two groups of subjects after the test
TABLE 5 blood pressure test results of the two groups of subjects before and after the test
As can be seen from tables 4 and 5, before and after the test, the test group and the control group have no significant difference (P >0.05) in the aspects of spirit, sleep, diet, defecation and urination, blood pressure (including systolic pressure SBP and diastolic pressure DBP) and the like, and the test shows that the test does not generate other adverse reactions and toxic and side reactions to the test subjects.
2. Changes in physical and laboratory indices:
the results of the hematological tests of the test and control subjects before and after the test are shown in tables 6 and 7.
TABLE 6 hematological test results of the first two groups of subjects
TABLE 7 results of hematological tests on two groups of subjects after the test
As can be seen from tables 6 and 7, the hematological test results of the two groups of subjects before and after the test are not abnormal compared with the results before the test, and the test shows that the test does not produce other adverse reactions and toxic and side effects on the subjects.
The results of biochemical serum measurements of the test and control subjects before and after the test are shown in tables 8 and 9.
TABLE 8 Biochemical serum test results of the first two groups of subjects
TABLE 9 Biochemical serum test results of two groups of subjects after the test
As can be seen from tables 8 and 9, before and after the test, the biochemical detection results of the serum of the two groups of subjects are not abnormal compared with the results before the test, and the test shows that the test does not produce other adverse reactions and toxic and side reactions to the subjects.
3. And (3) an efficacy index detection result:
the results of measuring the changes in the serum MDA content, SOD activity, and GSH-Px activity of the test subjects and the control subjects before and after the test are shown in Table 10, Table 11, and Table 12.
TABLE 10 results of MDA (nmol/mL) content change before and after the test
Note: test groups self-control P < 0.01.
As can be seen from Table 10, after the test group had eaten the present invention for 3 months, the percentage of MDA content reduction in serum was 3.76%, and before and after the test group had eaten the present invention, the MDA content in serum was significantly reduced, and the difference was significant (P < 0.01). After the placebo is eaten in the control group for 3 months, the MDA content in the serum is not obviously changed, and the reduction difference of the MDA content in the serum before and after the placebo is eaten has no significance (P is more than 0.05). After the test feeding, the MDA content in the serum of the test group is obviously increased compared with that of the control group, and the difference is significant (P is less than 0.01).
TABLE 11 detection results of SOD (U/mL) activity changes before and after the test
Note: test group self control P<0.01, control of test group and control group after test feeding#P<0.01。
As can be seen from Table 11, after the test group had eaten the present invention for 3 months, the activity of SOD in serum increased by 19.40%, and the activity of SOD in serum increased significantly before and after the test group had eaten, with significant difference (P < 0.01). After the placebo is eaten in the control group for 3 months, the activity of SOD in the serum of the control group is not obviously increased, and the activity increase difference of SOD in the serum before and after the placebo is eaten has no significance (P is more than 0.05). After the test food, the SOD activity in the serum of the test group is obviously increased compared with that of the control group, and the difference is significant (P < 0.01).
TABLE 12 detection results of GSH-Px enzyme (U/mL) activity changes before and after the test
Note: test group self control P<0.01, control of test group and control group after test feeding#P<0.01。
As can be seen from Table 12, after the test group takes the present invention for 3 months, the activity of GSH-Px in serum increases by 10.09%, and the activity of GSH-Px in serum increases obviously before and after the test group takes the present invention, with obvious difference (P < 0.01). After the placebo is eaten in the control group for 3 months, the activity of the GSH-Px in the serum of the control group is not obviously increased, and the activity of the GSH-Px in the serum before and after the placebo is eaten is not obviously increased (P is more than 0.05). After the test feeding, the activity of the GSH-Px in the serum of the test group is obviously increased compared with that of the control group, and the difference is significant (P < 0.01).
According to the human body test results, the following results are obtained: 1. before and after the test, compared with a control group, the test group has no obvious influence on the aspects of spirit, sleep, diet, excrement and urine, blood pressure (including systolic pressure SBP and diastolic pressure DBP), hematology detection, serum biochemical detection results and the like, and has no adverse reaction, thereby indicating that the invention has no toxic or side effect. 2. After the test feeding, the average level of the MDA content in the serum of the test subject is obviously reduced (P <0.01) compared with that before the test, the average level of the MDA content in the serum of the test subject is extremely lower than that of the control group (P <0.01), and the reduction percentage of the average level of the MDA content in the serum of the test subject is obviously different from that of the control group (P > 0.05); the mean level of SOD activity in the blood serum of a test subject is obviously improved (P <0.01) compared with that before the test, the mean level of SOD activity in the blood serum of the test subject is obviously higher than that of a control group (P <0.01), and the difference between the percentage of the SOD activity in the blood serum of the test subject and the percentage of the SOD activity in the control group is obvious (P > 0.05); the average level of the GSH-Px activity in the blood serum of the tested subjects in the test group is obviously increased compared with that before the test (P <0.01), the average level of the GSH-Px activity in the blood serum of the tested subjects is obviously increased compared with that of the control group (P <0.01), and the increase percentage of the GSH-Px activity in the blood serum of the tested subjects is obviously different from that of the control group (P > 0.05). Therefore, the invention can reduce the MDA content in human serum and improve the SOD activity and GSH-Px activity level in human serum, has the function of antioxidation, does not have adverse effect on the body health of a subject, and has excellent market popularization and application values.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.