CN107593771A - One kind preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals - Google Patents
One kind preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals Download PDFInfo
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Abstract
The present invention relates to one kind preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals, it is made up of following substances：Amino acid, kushenin, Zhongshengmycin and bacillus subtilis CGMCC2010146 zymotic fluids；The amino acid, kushenin, the ratio of Zhongshengmycin and bacillus subtilis CGMCC2010146 zymotic fluids are 2~5kg of amino acid, 50~150L of 0.5~1kg of kushenin parts, 0.01~0.5kg of Zhongshengmycin and withered grass gemma mixed fermentation liquid, and the withered grass gemma mixed fermentation liquid bacteria containing amount is 5 × 108~8 × 1010cfu/mL.Present invention applicant has found that kushenin, Zhongshengmycin and bacillus subtilis CGMCC2010146 zymotic fluids compounding can preferably prevent and treat tea moire blight by substantial amounts of screening study, active component kushenin, Zhongshengmycin and the combination of bacillus subtilis CGMCC2010146 zymotic fluids can have synergistic function, realize real nontoxic health pollution-free, that people will not be threatened, it is green safe, maintain the fine quality of tealeaves.
Description
Technical field
The present invention relates to one kind preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals, belong to for tea tree
Field of biological pesticide.
Background technology
Tea moire blight also known as leaf blight, mainly cause harm into leaf and old leaf, bud-leaf of also causing harm, branch and fruit.Tea cloud
Line leaf blight can occur throughout the year in warm area, and especially (more than 28 DEG C of temperature on average, relative humidity exists under the conditions of hot and humid
More than 80%) most useful for morbidity；Tea garden soil level of ground water height, impeded drainage, soil layer are shallower, and morbidity is heavier.Chemical pesticide because
Its wide in variety, quick, easy to use, lower-price characteristic and leading position is occupied in pesticide market.However, due to people
To the unreasonable use of chemical pesticide and agricultural chemicals inherently the shortcomings that, cause chemical pesticide to generate environment dirt in use
The problems such as dye, person poultry poisoning, residues of pesticides.With the raising of people's quality of life and the enhancing of environmental consciousness, develop to people and animals
Safe and pollution-free, less toxic, low-residual biological pesticide has turned into the new focus of pesticide field research.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of preventing and treating tea tree tea moire blight microorganism and plant source
Recompounded pesticide, overcome in the prior art tea chemistry residues of pesticides it is exceeded to being damaged risk using body.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：One kind preventing and treating tea tree tea moire blight microorganism and
Plant source compound agricultural chemicals, is made up of following substances：Amino acid, kushenin, Zhongshengmycin and bacillus subtilis
CGMCC2010146 zymotic fluids；The amino acid, kushenin, Zhongshengmycin and bacillus subtilis CGMCC2010146 zymotic fluids
Ratio be 2~5kg of amino acid, 0.5~1kg of kushenin parts, 0.01~0.5kg of Zhongshengmycin and withered grass gemma mixed fermentation
50~150L of liquid, the withered grass gemma mixed fermentation liquid bacteria containing amount are 5 × 108~8 × 1010cfu/mL。
Applicant has filtered out a kind of bacillus subtilis CGMCC2010146, June 13 in 2010 by a large amount of screenings
Day is preserved in China typical culture collection center (abbreviation CCTCC), preserving number：CCTCC M 2010146)
Above-mentioned bacillus subtilis CGMCC2010146 bacterium colony is white or faint yellow, opaque, circular, surface is thick
Rough, centre purses up, edge is irregular；It is aerobic, Gram-positive bacillus.The most suitable growth pH value：6.5~7.5, most suitable life
Long temperature：25-35℃.
The beneficial effects of the invention are as follows：Present invention applicant has found kushenin, Zhongshengmycin by substantial amounts of screening study
And bacillus subtilis CGMCC2010146 zymotic fluids compounding can preferably prevent and treat tea moire blight, active component kushenin,
Zhongshengmycin and the combination of bacillus subtilis CGMCC2010146 zymotic fluids have good synergistic function, realize really
Nontoxic health pollution-free, that people will not be threatened, it is green safe, maintain the fine quality of tealeaves.
On the basis of above-mentioned technical proposal, the present invention can also do following improvement.
Preventing and treating tea tree tea moire blight microorganism a kind of as described above of the invention and plant source compound agricultural chemicals, further,
Gemma amount is more than 80% in the bacillus subtilis CGMCC2010146 zymotic fluids.
Preventing and treating tea tree tea moire blight microorganism a kind of as described above of the invention and plant source compound agricultural chemicals, further,
The amino acid, kushenin, the ratio of Zhongshengmycin and withered grass gemma mixed fermentation liquid are amino acid 3kg, kushenin 1kg
Part, Zhongshengmycin 0.2kg and withered grass gemma mixed fermentation liquid 100L.
Preventing and treating tea tree tea moire blight microorganism a kind of as described above of the invention and plant source compound agricultural chemicals, further,
The amino acid comprises at least lysine, tryptophan, methionine, threonine and valine.
Preventing and treating tea tree tea moire blight microorganism a kind of as described above of the invention and plant source compound agricultural chemicals, further,
The lysine, tryptophan, methionine, threonine and valine weight ratio are 1:2:1.5:3:2.2.
It is using above-mentioned further beneficial effect：Definite value and fertility of the microorganism on tea free can be improved,
Antagonistic effect can be improved.
The present invention also provides a kind of preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals, including following step
Suddenly：Step 1), activation, bacillus subtilis CGMCC2010146 is activated using conventional method；
Step 2), the bacillus subtilis CGMCC2010146 of activation is bred using the experimental method of routine；
Step 3), according to 1:Cultured bacterial strain is moved into fermentation tank and carries out fermented and cultured by 200~250 ratio, is trained
Support fermentation time 24~48 hours, cultivation temperature is 25-35 DEG C, pH value 7.0~7.2；It is small every two in 24~48 hours
When detect fermentation tank in bacterial content；
Step 4), fermentation tank bacterial content are more than 5 × 106Cfu/mL, Number of spores are less than 30%, are enlarged culture, will
Zymotic fluid in tank is moved into large fermentation tank to be fermented respectively, fermentation time 24~48 hours, temperature within fermenting 24 hours
Degree maintains 25-35 DEG C, and more than 24 hours temperature controls of fermenting are in 36~39 DEG C, each two hour detection large fermentation tank
Zymotic fluid bacteria containing amount is 5 × 108~3 × 1010Cfu/mL, gemma amount is more than 80%, by bacillus subtilis after stirring
CGMCC2010146 zymotic fluids are according to any one of Claims 1-4 amino acid, kushenin, Zhongshengmycin and withered grass gemma
The ratio of CGMCC2010146 zymotic fluids carries out mixing and obtains Recompounded pesticide.
Every mu of tea place dilutes 300-1000 times of liquid using 30-50L and sprayed during use.It can also be applied by common method,
Such as spraying or pouring root, usage amount changes with weather condition or crop condition, and time of application can be before crop is susceptible or every afterwards
Mu tea place dilutes 300-1000 times of liquid using 30-50L and sprayed.
Embodiment
The principle and feature of the present invention are described with reference to embodiments, the given examples are served only to explain the present invention,
It is not intended to limit the scope of the present invention.
Embodiment 1
One kind preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals, are made up of following substances：Amino acid,
Kushenin, Zhongshengmycin and bacillus subtilis CGMCC2010146 zymotic fluids；The amino acid, kushenin, Zhongshengmycin and
The ratio of bacillus subtilis CGMCC2010146 zymotic fluids is amino acid 5kg, kushenin 0.5kg parts, Zhongshengmycin 0.01kg
And withered grass gemma mixed fermentation liquid 50L, the withered grass gemma mixed fermentation liquid bacteria containing amount are 5 × 108cfu/mL；It is described to rely
Propylhomoserin, tryptophan, methionine, threonine and valine weight ratio are 1:2:1.5:3:2.2.
Above-mentioned preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals, comprise the following steps：Step 1), it is living
Change, bacillus subtilis CGMCC2010146 is activated using conventional method；
Step 2), the bacillus subtilis CGMCC2010146 of activation is bred using the experimental method of routine；
Step 3), according to 1:Cultured bacterial strain is moved into fermentation tank and carries out fermented and cultured, cultivation and fermentation by 200 ratio
24 hours time, cultivation temperature are 35 DEG C, pH value 7.2；
Step 4), fermentation tank bacterial content are more than 5 × 106Cfu/mL, Number of spores are less than 30%, are enlarged culture, will
Zymotic fluid in tank is moved into large fermentation tank to be fermented respectively, and fermentation time 48 hours, temperature is tieed up within fermenting 24 hours
Hold at 25 DEG C, zymotic fluid army of more than the 24 hours temperature controls of fermenting in 36 DEG C, each two hour detection large fermentation tank contains
Bacterium amount is 3 × 1010Cfu/mL, gemma amount is more than 80%, by bacillus subtilis CGMCC2010146 zymotic fluids after stirring
Ratio according to above-mentioned amino acid, kushenin, Zhongshengmycin and withered grass gemma CGMCC2010146 zymotic fluids carries out mixing acquisition
Recompounded pesticide.
Test site is Xinyang City, Henan Province Shihe river ports town, tests 5 mu of crop field, and embodiment 1 is applied to preventing and treating tea moire leaf
Rot, before dispenser and eight days after second of dispenser investigate disease index.Embodiment 1 is sprayed after being diluted with water 800 times and applied, Yu Fa
Apply first time medicine sick initial stage, second of dispenser is carried out after 6 days, the prevention effect of 10 days is 81.4% after dispenser.
Test site is that 5 mu of crop field is tested in Xinyang City, Henan Province Shihe river ports town, and it is withered that amino acid is applied to preventing and treating tea moire leaf
Disease, the amount of amino acid are the amount of the amino acid in embodiment 1, and before dispenser and eight days after second of dispenser investigate disease index.It is real
Apply to spray after example 1 is diluted with water 800 times and apply, apply first time medicine in early stage, second of dispenser is carried out after 6 days, after dispenser
The prevention effect of 10 days is 1.4%.
Test site is that 5 mu of crop field is tested in Xinyang City, Henan Province Shihe river ports town, and it is withered that kushenin is applied to preventing and treating tea moire leaf
Disease, the dosage of kushenin are the amount of the kushenin in embodiment 1, and before dispenser and eight days after second of dispenser investigate disease index.
Embodiment 1 is sprayed after being diluted with water 800 times and applied, and applies first time medicine in early stage, second of dispenser, dispenser are carried out after 6 days
The prevention effect of 10 days is 7.4% afterwards.
Test site is that 5 mu of crop field is tested in Xinyang City, Henan Province Shihe river ports town, and Zhongshengmycin is applied to preventing and treating tea moire leaf
Rot, the dosage of Zhongshengmycin are the amount of the Zhongshengmycin in embodiment 1, and before dispenser and eight days after second of dispenser investigate the state of an illness
Index.Embodiment 1 is sprayed after being diluted with water 800 times and applied, and applies first time medicine in early stage, second of dispenser is carried out after 6 days,
The prevention effect of 10 days is 10.4% after dispenser.
Test site is that 5 mu of crop field, bacillus subtilis CGMCC2010146 hairs are tested in Xinyang City, Henan Province Shihe river ports town
Zymotic fluid is applied to preventing and treating tea moire blight, and the amount of bacillus subtilis CGMCC2010146 zymotic fluids is withered in embodiment 1
The amount of careless bacillus CGMCC2010146 zymotic fluids, before dispenser and eight days after second of dispenser investigate disease index.Embodiment 1
Spray and apply after being diluted with water 800 times, apply first time medicine in early stage, second of dispenser is carried out after 6 days, 10 days after dispenser
Prevention effect is 20.4%.
Embodiment 2
One kind preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals, are made up of following substances：Amino acid,
Kushenin, Zhongshengmycin and bacillus subtilis CGMCC2010146 zymotic fluids；The amino acid, kushenin, Zhongshengmycin and
The ratio of bacillus subtilis CGMCC2010146 zymotic fluids be amino acid 5kg, kushenin 0.8kg parts, Zhongshengmycin 0.5kg and
Withered grass gemma mixed fermentation liquid 150L, the withered grass gemma mixed fermentation liquid bacteria containing amount are 8 × 1010cfu/mL；；It is described to rely
Propylhomoserin, tryptophan, methionine, threonine and valine weight ratio are 1:2:1.5:3:2.2.
A kind of above-mentioned preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals, comprise the following steps：Step
1), activate, bacillus subtilis CGMCC2010146 is activated using conventional method；
Step 2), the bacillus subtilis CGMCC2010146 of activation is bred using the experimental method of routine；
Step 3), according to 1:Cultured bacterial strain is moved into fermentation tank and carries out fermented and cultured, cultivation and fermentation by 250 ratio
48 hours time, cultivation temperature are 32~36 DEG C, pH value 7.0~7.2；Detected in 48 hours every two hours in fermentation tank
Bacterial content；
Step 4), fermentation tank bacterial content are 9.2 × 106Cfu/mL, Number of spores are less than 30%, are enlarged culture, will
Zymotic fluid in tank is moved into large fermentation tank to be fermented respectively, fermentation time 24~48 hours, temperature within fermenting 24 hours
Degree maintains 32~36 DEG C, and more than 24 hours temperature controls of fermenting are in 37~39 DEG C, each two hour detection large fermentation tank
Zymotic fluid army bacteria containing amount be 8 × 1010Cfu/mL, gemma amount 90%, by bacillus subtilis after stirring
CGMCC2010146 zymotic fluids ferment according to above-mentioned amino acid, kushenin, Zhongshengmycin and the withered grass gemma CGMCC2010146 of stating
The ratio of liquid carries out mixing and obtains Recompounded pesticide.
Test site is Xinyang City, Henan Province Shihe river ports town, and embodiment 2 is applied to preventing and treating tea moire blight, before dispenser and
Eight days investigation disease indexs after second of dispenser.Embodiment 2 is sprayed after being diluted with water 300 times and applied, and first is applied in early stage
Secondary medicine, second of dispenser is carried out after 6 days, the prevention effect of 10 days is 87.4% after dispenser.
Test site is that 5 mu of crop field is tested in Xinyang City, Henan Province Shihe river ports town, and it is withered that amino acid is applied to preventing and treating tea moire leaf
Disease, the amount of amino acid are the amount of the amino acid in embodiment 1, and before dispenser and eight days after second of dispenser investigate disease index.It is real
Apply to spray after example 1 is diluted with water 800 times and apply, apply first time medicine in early stage, second of dispenser is carried out after 6 days, after dispenser
The prevention effect of 10 days is 2.1%.
Test site is that 5 mu of crop field is tested in Xinyang City, Henan Province Shihe river ports town, and it is withered that kushenin is applied to preventing and treating tea moire leaf
Disease, the dosage of kushenin are the amount of the kushenin in embodiment 1, and before dispenser and eight days after second of dispenser investigate disease index.
Embodiment 1 is sprayed after being diluted with water 800 times and applied, and applies first time medicine in early stage, second of dispenser, dispenser are carried out after 6 days
The prevention effect of 10 days is 5.4% afterwards.
Test site is that 5 mu of crop field is tested in Xinyang City, Henan Province Shihe river ports town, and Zhongshengmycin is applied to preventing and treating tea moire leaf
Rot, the dosage of Zhongshengmycin are the amount of the Zhongshengmycin in embodiment 1, and before dispenser and eight days after second of dispenser investigate the state of an illness
Index.Embodiment 1 is sprayed after being diluted with water 800 times and applied, and applies first time medicine in early stage, second of dispenser is carried out after 6 days,
The prevention effect of 10 days is 13.4% after dispenser.
Test site is that 5 mu of crop field, bacillus subtilis CGMCC2010146 hairs are tested in Xinyang City, Henan Province Shihe river ports town
Zymotic fluid is applied to preventing and treating tea moire blight, and the amount of bacillus subtilis CGMCC2010146 zymotic fluids is withered in embodiment 1
The amount of careless bacillus CGMCC2010146 zymotic fluids, before dispenser and eight days after second of dispenser investigate disease index.Embodiment 1
Spray and apply after being diluted with water 800 times, apply first time medicine in early stage, second of dispenser is carried out after 6 days, 10 days after dispenser
Prevention effect is 22.4%.
Embodiment 3
One kind preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals, are made up of following substances：Amino acid,
Kushenin, Zhongshengmycin and bacillus subtilis CGMCC2010146 zymotic fluids；The amino acid, kushenin, Zhongshengmycin and
The ratio of bacillus subtilis CGMCC2010146 zymotic fluids is amino acid, kushenin, Zhongshengmycin and withered grass gemma Mixed Microbes
The ratio of zymotic fluid is amino acid 3kg, kushenin 1kg parts, Zhongshengmycin 0.2kg and withered grass gemma mixed fermentation liquid 100L,
The withered grass gemma mixed fermentation liquid bacteria containing amount is 5.2 × 1010cfu/mL；The lysine, tryptophan, methionine, Soviet Union's ammonia
Acid and valine weight ratio are 1:2:1.5:3:2.2.
Preventing and treating tea tree tea moire blight microorganism a kind of as described above of the invention and plant source compound agricultural chemicals, including it is following
Step：Step 1), activation, bacillus subtilis CGMCC2010146 is activated using conventional method；
Step 2), the bacillus subtilis CGMCC2010146 of activation is bred using the experimental method of routine；
Step 3), according to 1:Cultured bacterial strain is moved into fermentation tank and carries out fermented and cultured by 200~250 ratio, is trained
Support fermentation time 24~48 hours, cultivation temperature is 32~36 DEG C, pH value 7.0~7.2；It is small every two in 24~48 hours
When detect fermentation tank in bacterial content；
Step 4), fermentation tank bacterial content are more than 5 × 106Cfu/mL, Number of spores are less than 30%, are enlarged culture, will
Zymotic fluid in tank is moved into large fermentation tank to be fermented respectively, fermentation time 24~48 hours, temperature within fermenting 24 hours
Degree maintains 32~36 DEG C, and more than 24 hours temperature controls of fermenting are in 37~39 DEG C, each two hour detection large fermentation tank
Zymotic fluid army bacteria containing amount be 5.2 × 1010Cfu/mL, gemma amount 95%, by bacillus subtilis after stirring
CGMCC2010146 zymotic fluids are according to above-mentioned amino acid, kushenin, Zhongshengmycin and withered grass gemma CGMCC2010146 zymotic fluids
Ratio carry out mixing obtain Recompounded pesticide.
Test site is Xinyang City, Henan Province Shihe river ports town, and embodiment 3 is applied to preventing and treating tea moire blight, before dispenser and
Eight days investigation disease indexs after second of dispenser.Embodiment 3 is sprayed after being diluted with water 500 times and applied, and first is applied in early stage
Secondary medicine, second of dispenser is carried out after 6 days, the prevention effect of 10 days is 89.4% after dispenser.
Test site is that 5 mu of crop field is tested in Xinyang City, Henan Province Shihe river ports town, and it is withered that amino acid is applied to preventing and treating tea moire leaf
Disease, the amount of amino acid are the amount of the amino acid in embodiment 1, and before dispenser and eight days after second of dispenser investigate disease index.It is real
Apply to spray after example 1 is diluted with water 800 times and apply, apply first time medicine in early stage, second of dispenser is carried out after 6 days, after dispenser
The prevention effect of 10 days is 1.1%.
Test site is that 5 mu of crop field is tested in Xinyang City, Henan Province Shihe river ports town, and it is withered that kushenin is applied to preventing and treating tea moire leaf
Disease, the dosage of kushenin are the amount of the kushenin in embodiment 1, and before dispenser and eight days after second of dispenser investigate disease index.
Embodiment 1 is sprayed after being diluted with water 800 times and applied, and applies first time medicine in early stage, second of dispenser, dispenser are carried out after 6 days
The prevention effect of 10 days is 8.9% afterwards.
Test site is that 5 mu of crop field is tested in Xinyang City, Henan Province Shihe river ports town, and Zhongshengmycin is applied to preventing and treating tea moire leaf
Rot, the dosage of Zhongshengmycin are the amount of the Zhongshengmycin in embodiment 1, and before dispenser and eight days after second of dispenser investigate the state of an illness
Index.Embodiment 1 is sprayed after being diluted with water 800 times and applied, and applies first time medicine in early stage, second of dispenser is carried out after 6 days,
The prevention effect of 10 days is 13.4% after dispenser.
Test site is that 5 mu of crop field, bacillus subtilis CGMCC2010146 hairs are tested in Xinyang City, Henan Province Shihe river ports town
Zymotic fluid is applied to preventing and treating tea moire blight, and the amount of bacillus subtilis CGMCC2010146 zymotic fluids is withered in embodiment 1
The amount of careless bacillus CGMCC2010146 zymotic fluids, before dispenser and eight days after second of dispenser investigate disease index.Embodiment 1
Spray and apply after being diluted with water 800 times, apply first time medicine in early stage, second of dispenser is carried out after 6 days, 10 days after dispenser
Prevention effect is 28.4%.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (10)
1. one kind preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals, are made up of following substances：Amino acid, hardship
Ginseng element, Zhongshengmycin and bacillus subtilis CGMCC2010146 zymotic fluids；The amino acid, kushenin, Zhongshengmycin and withered
The ratio of careless bacillus CGMCC2010146 zymotic fluids is 2~5kg of amino acid, 0.5~1kg of kushenin parts, Zhongshengmycin
50~150L of 0.01~0.5kg and withered grass gemma mixed fermentation liquid, the withered grass gemma mixed fermentation liquid bacteria containing amount be 5 ×
108~8 × 1010cfu/mL。
2. a kind of preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals according to claim 1, its feature
It is, gemma amount is more than 80% in the bacillus subtilis CGMCC2010146 zymotic fluids.
3. a kind of preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals according to claim 1, its feature
It is, the amino acid, kushenin, the ratio of Zhongshengmycin and withered grass gemma mixed fermentation liquid are amino acid 3kg, kushenin
1kg parts, Zhongshengmycin 0.2kg and withered grass gemma mixed fermentation liquid 100L.
4. a kind of preparation side for preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals is stated according to claim 1
Method, it is characterised in that the amino acid comprises at least lysine, tryptophan, methionine, threonine and valine.
5. a kind of preparation side for preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals is stated according to claim 4
Method, it is characterised in that the lysine, tryptophan, methionine, threonine and valine weight ratio are 1:2:1.5:3:
2.2。
A kind of 6. preparation method for preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals, it is characterised in that bag
Include following steps：Step 1), activation, bacillus subtilis CGMCC2010146 is activated using conventional method；
Step 2), the bacillus subtilis CGMCC2010146 of activation is bred using the experimental method of routine；
Step 3), according to 1:Cultured bacterial strain is moved into fermentation tank and carries out fermented and cultured by 200~250 ratio, culture hair
24~48 hours ferment time, cultivation temperature are 25-35 DEG C, pH value 7.0~7.2；Examined in 24~48 hours every two hours
Survey bacterial content in fermentation tank；
Step 4), fermentation tank bacterial content are more than 5 × 106Cfu/mL, Number of spores are less than 30%, culture are enlarged, by tank
Zymotic fluid is moved into large fermentation tank to be fermented respectively, and fermentation time 24~48 hours, temperature maintains within fermenting 24 hours
At 25-35 DEG C, zymotic fluid of more than the 24 hours temperature controls of fermenting in 36~39 DEG C, each two hour detection large fermentation tank
Bacteria containing amount is 5 × 108~3 × 1010Cfu/mL, gemma amount is more than 80%, by bacillus subtilis after stirring
CGMCC2010146 zymotic fluids are according to any one of Claims 1-4 amino acid, kushenin, Zhongshengmycin and withered grass gemma
The ratio of CGMCC2010146 zymotic fluids carries out mixing and obtains Recompounded pesticide.
7. a kind of preparation side for preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals is stated according to claim 6
Method, it is characterised in that step 4) detection gemma amount is more than 95%, after zymotic fluid is excluded with amino acid, kushenin and middle raw bacterium
Element proportionally mixes.
8. a kind of preparation side for preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals is stated according to claim 6
Method, it is characterised in that the amino acid comprises at least lysine, tryptophan, methionine, threonine and valine.
9. a kind of preparation side for preventing and treating tea tree tea moire blight microorganism and plant source compound agricultural chemicals is stated according to claim 6
Method, it is characterised in that the lysine, tryptophan, methionine, threonine and valine weight ratio are 1:2:1.5:3:
2.2。
10. a kind of method for preventing and treating tea tree tea moire blight microorganism and plant source compound pesticide control rice sheath blight disease, its
It is characterised by, every mu of tea place dilutes 300-1000 times of liquid using 30-50L and sprayed during use.
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| CN107522571A (en) * | 2017-06-20 | 2017-12-29 | 上海农乐生物制品股份有限公司 | A kind of organic function nutrition fertilizer and preparation method thereof |
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