CN107593709A - A kind of Antiphytoviral composition and its application - Google Patents
A kind of Antiphytoviral composition and its application Download PDFInfo
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- CN107593709A CN107593709A CN201710978262.9A CN201710978262A CN107593709A CN 107593709 A CN107593709 A CN 107593709A CN 201710978262 A CN201710978262 A CN 201710978262A CN 107593709 A CN107593709 A CN 107593709A
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Abstract
The present invention discloses a kind of Antiphytoviral composition and its application, and the active component of the Antiphytoviral composition includes：Amino-oligosaccharide and epiphysin；The amino-oligosaccharide and the epiphysin mass ratio are (1~50)：1；The Antiphytoviral composition for preventing and controlling virosis effect is good, rapid-action, improves crop immunity, may additionally facilitate the growth of crop system.
Description
Technical field
The present invention relates to technical field of pesticide, and in particular to a kind of Antiphytoviral composition and its application.
Background technology
The viroses of plant are by the parasitogenic disease of plant virus.Except capturing by addition to infecting a part of nutrition of plant,
The duplication of nucleic acid (RNA or DNA) and protein coat is carried out in host cell, forms new virion.Phytopathy virion
Body or viral nucleic acid can shift rapidly in vascular bundle with the nutrition flow direction of plant, the plant whole body is fallen ill, mainly may be used
Change the normal metabolic processes of host plant, disturb or destroy its respiration, photosynthesis, the activity of enzyme, and auxin
With the metabolism of other hormones etc..
Plant virus species is various, and hazard area is wide, and preventing and treating is difficult, up to the present still without effective medicament,
Mainly or based on selecting antiviral kind, strengthen the comprehensive preventive health measures such as cultivation management, seed disinfection.Once infect, very
Hardly possible is prevented and treated with medicament.
The content of the invention
In view of this, this application provides a kind of Antiphytoviral composition and its application, the Antiphytoviral combination
Thing preventing and treating virosis effect is good, rapid-action, improves crop immunity, may additionally facilitate the growth of crop system.
To solve above technical problem, technical scheme provided by the invention is a kind of Antiphytoviral composition, described anti-
The active component of plant virus composition includes：Amino-oligosaccharide and epiphysin；The amino-oligosaccharide and the epiphysin matter
It is (1~50) to measure ratio：1.
Preferably, the amino-oligosaccharide and the epiphysin mass ratio are (1~20)：1.
Preferably, the amino-oligosaccharide and the epiphysin mass ratio are (1~10)：1.
Preferably, be calculated in mass percent, the active component in the Antiphytoviral composition content be 0.1~
20%.
Preferably, be calculated in mass percent, the active component in the Antiphytoviral composition content be 0.5~
10%.
Preferably, the Antiphytoviral composition also includes：Acceptable auxiliary material composition in Pesticide Science.
Preferably, the Antiphytoviral combination dosage form is soluble concentrate, solvable pulvis, emulsifiable granula, wettable
Pulvis or water dispersible granules.
Preferably, the Antiphytoviral combination dosage form is soluble concentrate or solvable pulvis；It is convenient to be easy to use.
Preferably, the accessory package is containing at least two in cosolvent, solvent, surfactant, filler.
Preferably, the percentage that the cosolvent and the solvent gross weight account for the Antiphytoviral medicament gross weight is
1~70%.
Preferably, the percentage that the surfactant weight accounts for the Antiphytoviral medicament gross weight is 1-10%,
Preferably, the percentage that the filler weight accounts for the Antiphytoviral medicament gross weight is 1~90%.
Preferably, the solvent is selected from water, ethylene glycol, isopropanol, dibasic ester, tetrahydrofurfuryl alcohol, propane diols, N, N- diformazans
One or more in base quinoline acid amides, 1-METHYLPYRROLIDONE, dimethyl sulfoxide (DMSO), phenmethylol, benzyl carbinol.
Preferably, the cosolvent is selected from selected from water, ethylene glycol, isopropanol, dibasic ester, tetrahydrofurfuryl alcohol, propane diols, N,
One or more in N- dimethyl quinolines acid amides, 1-METHYLPYRROLIDONE, dimethyl sulfoxide (DMSO), phenmethylol, benzyl carbinol.
Preferably, the surfactant is selected from Tween 80, TERMUL5036, castor oil polyoxyethylene ether, dodecyl
Sodium sulphate, phenethyl phenol polyethenoxy ether, butanedioic acid salt, polyethylene glycol aryl diphenyl ether, alkylnaphthalene sulfonate formaldehyde condensation
In thing, alkyl naphthyl sodium sulfate salt, succinic acid Diisooctyl sodium sulfonate, EO/PO polyethers, polycarboxylate, sodium lignin sulfonate
It is one or more.
Preferably, the surfactant is selected from Tween 80, TERMUL5036, phenethyl phenol polyethenoxy ether, alkylnaphthalene
Sulfonate and formaldehyde condensation product, alkyl naphthyl sodium sulfate salt, succinic acid Diisooctyl sodium sulfonate, polycarboxylate, sodium lignin sulfonate,
One or more in lauryl sodium sulfate.
Above-mentioned TERMUL5036 is the mixture of alkyl, polyether and alkylsulfonate.The alkyl, polyether and the alkyl sulphur
Hydrochlorate weight ratio is (1~10)：1.
Preferably, the filler is selected from white carbon, anhydrous magnesium sulfate, borax, poly- Boratex, tripolyphosphate, pyrophosphoric acid
One or more in salt, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium sulfate, water soluble starch, calcium nitrate, potassium nitrate.
The present invention also provides a kind of application according to above-mentioned Antiphytoviral composition for preventing and controlling crop virosis.
Preferably, the crop is tobacco, veterinary antibiotics, flowers.
Preferably, the vegetables are capsicum.
Preferably, the fruit is watermelon.
Preferably, the flowers are fragrant thoroughwort.
Preferably, the virus-virus source includes：Tobacco mosaic virus (TMV), cucumber mosaic virus, watermelon mosaic virus, gladiolus
Mosaic virus.
Preferably, the concentration of the Antiphytoviral composition is 10~1000mg/Kg.
Preferably, the concentration of the Antiphytoviral composition is 50~500mg/Kg.
Preferably, the application process of the Antiphytoviral composition is：Fallen ill early stage or first in the crop virosis
Phase applies.
Preferably, the application process of the Antiphytoviral composition is the one or more in root dipping, spraying and pouring root.
Amino-oligosaccharide refers to D- Glucosamines with the oligosaccharide of β -1.4 glucosides key connections, can be passivated virus activity,
Viral interference RNA synthesis, hypha form morphs, biochemistry changes in spore for induction；Plant cell is activated, excites plant
Internal gene, quickly causes NO, H2O2、Ca2+And the generation of the semiochemicals such as jasmonic, so as to cause the outburst of active oxygen, lure
Chitinase activity in plant is led, strengthens plant itself anti-virus ability, hinders virion to be combined with each other, destroy germ
Cell membrane, the growth for hindering germ spore-germination and germ.Amino-oligosaccharide to fungal disease except having killing action, moreover it is possible to
Promote well developed root system, promote the absorption of Plant To Nutrient element, strengthen crop immunity and disease resistance, promote production and increase income；And sterilize
Recover the normal growing environment of plant afterwards, suppress the secondary outburst of disease.
Epiphysin is endogenous amine bormones, to the nontoxic noresidue of plant and animal.External source epiphysin has to plant
There are following functions：Adjust plant circadian rhythm and photoperiod；Promote plant establishment；Seed germination rate can be improved；With to plant
Anti-oxidant, light protection, anti-aging, anti-disease effect；Defensive ability/resistance ability of the plant to environment stress can be improved.
Compared with prior art, its detailed description is as follows by the application：
Compared with prior art, Antiphytoviral composition provided by the invention is black with taking off using amino-oligosaccharide by the application
Element compounding, preventing and treating virosis effect is good, rapid-action, to improve plant immunity, duration length, low toxicity, low-residual, to environment
It is friendly；Synergistic effect is shown compared to amino-oligosaccharide single dose and epiphysin single dose；In the case of identical preventive effect, with list
Agent is compared, and composition can reduce dosage, so as to reduce the influence to environment.Antiphytoviral composition provided by the invention
It is preferred that concentration, application process, further improve preventive effect.Antiphytoviral composition provided by the invention can promote root of the crop
System's growth, promote crop quickly healthy.In addition, applicable crops, which can prevent and treat virus, has broad spectrum activity.
Embodiment
In order that those skilled in the art more fully understands technical scheme, with reference to specific embodiment pair
The present invention is described in further detail.
Embodiment 1
Tobacco virus indoor bioassay is tested
1 materials and methods
1.1 vegetable materials and culture
Host：Nicotiana glutinosa (Nicotianaglutinosa).0.1% (mass concentration, similarly hereinafter) silver nitrate of tobacco seed
10~15min is sterilized, is then rinsed 3~4 times with flowing water, is seeded in matrix.Tobacco plant is incubated at during whole experiment
In the greenhouse of (25 ± 2) DEG C.Seedling separation after about 4 weeks, transplant in diameter 6cm, high 15cm plastic cup in, treat that seedling is grown to 5 leaves
Phase can carry out different processing.
1.2 for prelibation source and inoculation method
Tobacco mosaic virus (TMV) (TMV) is provided by China Agricultural University's virus research room.TMV inoculum densities are 25 μ g/mL, often
Strain 4 young leaves of inoculation.First pinched during inoculation, then uniformly spray the gold that a thin layer granularity is 38 μm in the blade surface that need to be inoculated with
Emery, with dipping in virulent writing brush gently uniform scrubbing brush blade surface.Method uses half leaf withered spot method.After virus inoculation 3-5d,
Withered spot number is counted, calculates preventive effect.
1.3 reagent agent
0.5% amino-oligosaccharide aqua：It is calculated in mass percent, amino-oligosacchride cellulose content is 0.5% in aqua；
0.5% epiphysin soluble concentrate：It is calculated in mass percent, melatonin content is 0.5% in soluble concentrate；
0.5% amino-oligosaccharide epiphysin (1：1) soluble concentrate：It is calculated in mass percent, amino is few in soluble concentrate
Sugared element and epiphysin total content are 0.5%, and amino-oligosaccharide is 1 with epiphysin weight ratio:1.
1.4 test method
Vaccination ways are used as two kinds：
Treatment：First virus inoculation, spray pharmacy again after 12h；
Protect (prevention)：First spray, virus is inoculated after 12h；
Every kind of concentration at least handles 3 plants, and the inhibiting rate of withered spot host uses half leaf withered spot method to be calculated with equation below, with
Average inhibition is evaluated.
Withered spot inhibiting rate=(control withered spot number-processing withered spot number)/control withered spot number × 100%
1.4.1 live body protection (prevention) Experiment on Function that medicament infects to TMV
The Nicotiana glutinosa that growing way is consistent is selected, gently spreading aqua sterilisa in Zuo Banye with writing brush compares, and right half leaf spreads medicament,
After blade is dry (after 24h), virus inoculation.Viral juice is dipped with writing brush, artificial frictional inoculation is in the blade sprinkled with diamond dust
On.
1.4.2 the live body therapeutic action experiment that medicament infects to TMV
The Nicotiana glutinosa that growing way is consistent is selected, first viral juice full leaf virus inoculation is dipped with writing brush, is rinsed after inoculation with water.Treat
After blade is dry, spreads aqua sterilisa in Zuo Banye and compare, right half leaf spreads medicament.Withered spot number is recorded after 3~5d.
2 result of the tests
Tobacco protection (prevention) effect that 2.1 medicaments infect to TMV：It is shown in Table 1
The tobacco therapeutic action that 2.2 medicaments infect to TMV：It is shown in Table 2
The tobacco protection and therapeutic action that 2.3 medicaments infect to TMV are compared
Protection (prevention) effect of the medicament of table 1 to tobacco TMV
Therapeutic effect of the medicament of table 2 to tobacco TMV
From Tables 1 and 2, two kinds of medicaments serve certain protection and therapeutic effect, composition to TMV live bodies
0.5% 100-500 times of amino-oligosacchride epiphysin soluble concentrate is all preferable to the inhibiting rate of virus, and inhibiting rate is all under each concentration
More than 60%, inhibiting rate has reached 78.2% under maximum concentration.The amino-oligosacchride aqua of single dose 0.5%, 0.5% epiphysin are solvable
The inhibiting rate of liquor maximum concentration is 63.4%, under remaining concentration substantially below 60%.Under each concentration, the present invention provides
Antiphytoviral composition drug effect compared with single dose, reached significant difference.
Contrasted from Tables 1 and 2, Antiphytoviral composition provided by the invention has protection (pre- to TMV live bodies
It is anti-) and therapeutic action.But comparatively, protection (prevention) effect is above therapeutic action, that is, anti-plant provided by the invention
The application process of virus composition is preferably：Fallen ill early stage or administration at initial stage in the crop virosis.It is provided by the invention
Antiphytoviral composition is protected and therapeutic effect is superior to amino-oligosacchride single dose and epiphysin single dose, and in different dilution times
Under several, between Antiphytoviral composition provided by the invention and lentinan single dose, Antiphytoviral group provided by the invention
Difference has the meaning of conspicuousness between compound and epiphysin single dose.
Embodiment 2
Pepper virus disease controlling experiment
1 materials and methods
1.1 test plant:Capsicum (peppery rich No. three), amranth multitude.
1.2 for examination virus:Cucumber mosaic virus (cucumber mosaic virus CMV), 1g virus blades during inoculation
10mlPBS buffer solutions are added to grind, with standby after filtered through gauze.
1.3 reagent agent
1.1% amino-oligosaccharide aqua：It is calculated in mass percent, amino-oligosacchride cellulose content is 1.1% in aqua；
1.1% epiphysin aqua：It is calculated in mass percent, melatonin content is 1.1% in soluble concentrate；
1.1% amino-oligosaccharide epiphysin (10：1) soluble concentrate：It is calculated in mass percent, amino is few in soluble concentrate
Sugared element and epiphysin total content are 1.1%, and amino-oligosaccharide is 10 with epiphysin weight ratio:1.
1.4 test method
1.4.1 indoor bioassay
Using half leaf method to amranth multitude's artificial infection CMV.24h is to processing amranth multitude blade difference before inoculation and after inoculation
Medicament is spread, 3 leaves of every plant of processing, 10 plants is often handled, is repeated 3 times.Investigation spreads the half leaf of medicament and is not coated with after 7-10d
The withered spot number of the half leaf of dispenser agent, and inhibiting rate is calculated according to the withered spot number on blade and withered spot diameter.
Withered spot inhibiting rate=(control withered spot number-processing withered spot number)/control withered spot number × 100%
1.4.2 field plot trial
1.4.2.1 experimental design
Each processing is repeated 3 times, each cell 20m2(2.5m × 4m), random alignment.In addition to chemicals treatment factor, other
Control measures are consistent.Before experiment and during, trial zone does not apply other medicaments to pepper virus disease with preventive and therapeutic effect.
1.4.2.2 spraying time and method
By above-mentioned medicament usage amount, in capsicum initial bloom stage first time medicine, 5d is spaced, with second of medicine, then 5d is spaced, uses
Third time medicine.Blank district does not make any chemicals treatment.
1.4.2.3 control time and method
Determine 10 plants of investigation before dispenser and per 5 points of samplings of cell, after third time dispenser at every, calculate disease index and preventing and treating
Effect.
Severity Scaling standard:
0 grade：Plant is normal, and blade is without symptom；
1 grade：Light floral leaf, Ming Mai, plant are not downgraded；
3 grades：Floral leaf is obvious, and shrinkage that blade is slight, plant is not downgraded；
5 grades：Weight floral leaf, crease blade disease, deformity, plant are slightly downgraded；
7 grades：Weight floral leaf, petiole, stem or total bennet have streak bad
Extremely, fruit piebald, the contracting of flower strain clump, stunt；
9 grades：Line leaf, seriously morbidity is downright bad to complete stool for stem.
Calculation formula:
Disease index=∑ (Ai × i)/M × 9 × 100
Note：Ai --- i level diseased plant numbers
I=0,1,3,5,7,9
M --- investigation total strain number
Prevention effect (%)=(1-CK0 × PT1/CK1 × PT0) × 100
Note：CK0 --- blank district disease refers to before dispenser
CK1 --- blank control disease refers to after dispenser
PT0 --- dispenser pre-treatment area disease refers to
PT1 --- dispenser post processing zone disease refers to
2. result of the test
2.1 indoor bioassay results
2.1.1 capsicum (protection (prevention) effect that medicament infects to CMV：Processing amranth multitude blade is applied before CMV inoculations
Dispenser agent, the results are shown in Table 3
2.1.2 the capsicum therapeutic action that medicament infects to CMV：Medicament is spread to processing amranth multitude's blade after CMV inoculations,
It the results are shown in Table 4
2.2 drug effect test of field zone：Use the softwares of SAS 8.0 with Deng Kenshi duncan's new multiple range methods to disease index progress side
Difference is analysed, and the results are shown in Table 5.
Protection (prevention) effect of the medicament of table 3 to CMV
Therapeutic effect of the medicament of table 4 to CMV
Pepper virus disease disease is referred to the medicament of table 5 and prevention effect
Above test data shows：The preventing and treating virosis effect that medicament is spread before composition inoculation provided by the invention compares
The preventive effect that medicament is spread after inoculation is good.That is, the application process of Antiphytoviral composition provided by the invention is preferably：In
The crop virosis morbidity early stage or initial stage apply.Protection of the Antiphytoviral composition provided by the invention to CMV is (pre-
It is anti-) effect, CMV therapeutic effect, pepper virus disease field efficacy be notable, better than same extension rate amino-oligosaccharide single dose and
Epiphysin single dose, and under different extension rates, between Antiphytoviral composition provided by the invention and amino-oligosaccharide single dose,
Difference has the meaning of conspicuousness between Antiphytoviral composition provided by the invention and epiphysin single dose.
Embodiment 3：
Watermelon mosaic virus disease controlling experiment
1st, for studying thing:Watermelon (8424).
2nd, controlling object:Watermelon mosaic virus (WMV), it is simultaneous to eliminate aphis.
3rd, reagent agent：
4.2% amino-oligosaccharide SP：It is calculated in mass percent, amino-oligosacchride cellulose content is 4.2% in solvable pulvis；
4.2% epiphysin WP：It is calculated in mass percent, melatonin content is 4.2% in wettable powder；
4.2% amino-oligosaccharide epiphysin (20：1)SP：Be calculated in mass percent, in solvable pulvis amino-oligosaccharide and
Epiphysin total content is 4.2%, and amino-oligosaccharide is 20 with epiphysin weight ratio:1.
350g/L imidacloprid suspending agents
4 experimental designs
4.1 experiments, supporting 1000 times of 350g/L imidacloprid suspending agents, and clear water control are each handled, often processing weight
It is multiple 3 times, each cell 32m2(4m × 8m), random alignment.In addition to chemicals treatment factor, other rich water quality management measures are consistent.
4.2 application method：
Medicament in watermelon seedling stage, stretch climing phase pouring root, the 7-10 weeks pouring root in interval once, per growth period pouring root 2-3 time, filling every time
Root is drenched by root and is defined.While each concentration processing, using the 1000 times of complete stool sprays preventing and treatings of 350g/L imidacloprid suspending agents
Any chemicals treatment is not made in aphid, blank control.
4.3 control times and method
Every 5 points of cell samples before dispenser and after last time dispenser, every fixed 10 plants of investigation, calculates disease index and prevents
Control effect.
Severity Scaling standard:
0 grade：Plant is normal, and blade is without symptom；
1 grade：Light floral leaf, Ming Mai, plant are not downgraded；
3 grades：Floral leaf is obvious, and shrinkage that blade is slight, plant is not downgraded；
5 grades：Weight floral leaf, crease blade disease, deformity, plant are slightly downgraded；
7 grades：Weight floral leaf, petiole, stem or total bennet have streak downright bad, fruit piebald, spend strain clump to contract, stunt；
9 grades：Line leaf, seriously morbidity is downright bad to complete stool for stem.
Calculation formula:
Disease index=∑ (Ai × i)/M × 9 × 100
Note：Ai --- i level diseased plant numbers
I=0,1,3,5,7,9
M --- investigation total strain number
Prevention effect (%)=(1-CK0 × PT1/CK1 × PT0) × 100
Note：CK0 --- blank district disease refers to before dispenser
CK1 --- blank control disease refers to after dispenser
PT0 --- dispenser pre-treatment area disease refers to
PT1 --- dispenser post processing zone disease refers to
5. drug effect test of field zone result：The softwares of SAS 8.0 are used to enter with Deng Kenshi duncan's new multiple range methods to disease index
Row variance analysis, the results are shown in Table 6.
Watermelon mosaic virus disease disease is referred to the medicament of table 6 and prevention effect
Above test data shows：Antiphytoviral composition provided by the invention is notable to watermelon mosaic virus preventive effect,
Better than the amino-oligosaccharide single dose and epiphysin single dose of same extension rate, and under different extension rates, anti-plant provided by the invention
Between thing virus composition and amino-oligosaccharide single dose, between Antiphytoviral composition provided by the invention and epiphysin single dose
Difference has the meaning of conspicuousness.
The fragrant thoroughwort virus disease control of embodiment 4 is tested
1st, for studying thing:Gladiolus.
2nd, controlling object:Gladiolus mosaic virus (CyMV).
3rd, reagent agent：
The solvable pulvis of 5.1% amino-oligosaccharide：It is calculated in mass percent, amino-oligosacchride cellulose content is in solvable pulvis
5.1%；
5.1% epiphysin WP：It is calculated in mass percent, melatonin content is 5.1% in wettable powder；
5.1% amino-oligosaccharide epiphysin (50：1) solvable pulvis：It is calculated in mass percent, amino is few in solvable pulvis
Sugared element and epiphysin total content are 5.1%, and amino-oligosaccharide is 50 with epiphysin weight ratio:1.
70% acetamiprid water dispersible granules
4 experimental designs
4.1 experiments each handle supporting 2000 times of 70% acetamiprid water dispersible granules, often handle 10 plants, random alignment.
In addition to chemicals treatment factor, other rich water quality management measures are consistent (including should to be carried out high temperature to media such as flowerpot, peat, chads and disappear
The control measures such as poison).
4.2 application method：
In gladiolus seedling stage, plant division phase, leaf stretching period, florescence, respectively once, method of application is that complete stool sprays add root to medicament for processing
Portion pours.While each concentration processing, eliminated aphis using 70% acetamiprid water dispersible granules, 2000 times of complete stool sprays are anti-, blank
Any chemicals treatment is not made in control.
4.3 control times and method
Investigated before dispenser and to 10 plants of gladiolus of each processing after last time dispenser, calculate disease index and preventing and treating is imitated
Fruit.
Severity Scaling standard:
0 grade：Plant is normal, and blade is without symptom；
1 grade：Light floral leaf, Ming Mai, plant are not downgraded；
3 grades：Floral leaf is obvious, and shrinkage that blade is slight, plant is not downgraded；
5 grades：Weight floral leaf, crease blade disease, deformity, plant are slightly downgraded；
7 grades：Weight floral leaf, petiole, stem or total bennet have streak downright bad, spend the contracting of strain clump, stunt；
9 grades：Line leaf, seriously morbidity is downright bad to complete stool for stem.
Calculation formula:
Disease index=∑ (Ai × i)/M × 9 × 100
Note：Ai --- i level diseased plant numbers
I=0,1,3,5,7,9
M --- investigation total strain number
Prevention effect (%)=(1-CK0 × PT1/CK1 × PT0) × 100
Note：CK0 --- blank district disease refers to before dispenser
CK1 --- blank control disease refers to after dispenser
PT0 --- dispenser pre-treatment area disease refers to
PT1 --- dispenser post processing zone disease refers to
Gladiolus mosaic virus disease is referred to the medicament of table 7 and prevention effect
Above test data shows：Antiphytoviral composition provided by the invention is notable to watermelon mosaic virus preventive effect,
Better than amino-oligosaccharide single dose and epiphysin single dose, Antiphytoviral composition provided by the invention and amino-oligosaccharide single dose are poor
Antiphytoviral composition different, provided by the invention has the meaning of conspicuousness with epiphysin single dose difference.
Embodiment 5
Virus disease control is tested
1st, for studying thing:Garland chrysanthemum.
1.2 for examination virus:Cucumber mosaic virus (cucumber mosaic virus CMV), 1g virus blades during inoculation
10mlPBS buffer solutions are added to grind, with standby after filtered through gauze.
1.3 reagent agents are shown in Table 8
1.4 test method
1.4.1 indoor bioassay
Using half leaf method artificial infection CMV.24h spreads medicament, every plant of processing 3 to processing garland chrysanthemum blade respectively before inoculation
Piece leaf, 10 plants are often handled, is repeated 3 times.
The half leaf of medicament is spread according to the investigation of the time of table 8 and is not coated with the withered spot number of the half leaf of dispenser agent, and according to leaf
Withered spot number and withered spot diameter on piece calculate inhibiting rate.
Withered spot inhibiting rate=(control withered spot number-processing withered spot number)/control withered spot number × 100%
Every plant of root long is investigated according to the time of table 9, analyzed using DPS6.55 (Duncan) statistical software.According to experiment
Average root long C1 is calculated in data, relative to the actual root long increment rate T1 of blank control.Combination is obtained using Colby formula
Physics discusses root long increment rate E1.The difference of actual value and theoretical value is more than 10% effect for Synergistic, actual value and reason
By effect for addition of the difference of value between ± 10%, the difference of actual value and theoretical value less than -10% for antagonism
Effect.
Colby formula：E=XY/100；X, Y is the actual increase rate obtained by the alone each component of difference.
2. result of the test
Garland chrysanthemum protection (prevention) effect that 2.1 medicaments infect to CMV：It the results are shown in Table 8
2.2 medicaments are shown in Table 9 to the action effect of crop root growth.
Protection (prevention) effect of the medicament of table 8 to CMV
Action effect of the medicament of table 9 to crop root growth
Above test data shows：Amino-oligosaccharide single dose is poor using slowly effect, preventive effect, and duration of efficacy is short；And this
The Antiphytoviral composition provided is provided, uses weight ratio as (1~50)：1 amino-oligosaccharide and epiphysin compounded combination
Thing concentration is that 10~1000mg/Kg applies preventing and treating crop virosis, on the one hand, shortens drug effect onset time, drug effect is lasting
Time is grown；On the other hand, preventive effect is significantly better than amino-oligosaccharide single dose and epiphysin single dose, Antiphytoviral provided by the invention
Difference has and shown between composition and amino-oligosaccharide single dose, between Antiphytoviral composition provided by the invention and epiphysin
The meaning of work property.In addition, crop root growth can be obviously promoted.Preferably, weight ratio is (1~20)：1；It is furthermore preferred that weight
Than for (1~10)：1；Preferably, composition concentration is 50~500mg/Kg.
Embodiment 6
0.5% amino-oligosaccharide epiphysin soluble concentrate
Amino-oligosaccharide：0.25%
Epiphysin：0.25%
T-80:3.0%
Ethanol：10.0%
Pure water：Complement to 100%
Embodiment 7
1.1% amino-oligosaccharide epiphysin soluble concentrate
Amino-oligosaccharide：1.0%
Epiphysin：0.1%
Isopropanol：10.0%
TERMUL5036：2.0%
Distilled water：Complement to 100%
Embodiment 8
The 4.2% solvable pulvis of amino-oligosaccharide epiphysin
Amino-oligosaccharide：4.0%
Epiphysin：0.2%
Anhydrous magnesium sulfate：5.0%
Sodium lignin sulfonate：5.0%
Boric acid：10.0%
Potassium dihydrogen phosphate：Complement to 100%
Embodiment 9
The 5.1% solvable pulvis of amino-oligosaccharide epiphysin
Amino-oligosaccharide：5.0%
Epiphysin：0.1%
Alkyl naphthyl sodium sulfate salt：3.0%
White carbon：2.0%
Anhydrous magnesium sulfate：5.0%
Potassium dihydrogen phosphate：20%
Water soluble starch：5.0%
Potassium sulfate：Complement to 100%
Embodiment 10
0.1% amino-oligosacchride epiphysin soluble concentrate
Amino-oligosacchride：0.05%
Epiphysin：0.05%
JFC bleeding agents:3.0%
Ethanol：10.0%
Pure water：Complement to 100%
Embodiment 11
10% amino-oligosacchride epiphysin wettable powder
Amino-oligosacchride：5.0%
Epiphysin：5.0%
Sodium lignin sulfonate：8.0%
Lauryl sodium sulfate：2.0%
White carbon：5.0%
Kaolin：Complement to 100%
It the above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
The limitation of the present invention, protection scope of the present invention should be defined by claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, some improvements and modifications can also be made, these change
Enter and retouch and also should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of Antiphytoviral composition, it is characterised in that the active component of the Antiphytoviral composition includes：Amino
Oligosaccharide and epiphysin；The amino-oligosaccharide and the epiphysin mass ratio are (1~50)：1.
2. Antiphytoviral composition according to claim 1, it is characterised in that the amino-oligosaccharide with it is described take off it is black
Plain mass ratio is (1~20)：1.
3. Antiphytoviral composition according to claim 1, it is characterised in that the amino-oligosaccharide with it is described take off it is black
Plain mass ratio is (1~10)：1.
4. Antiphytoviral composition according to claim 1, it is characterised in that be calculated in mass percent, the activity
Composition content in the Antiphytoviral composition is 0.1~20%.
5. Antiphytoviral composition according to claim 1, it is characterised in that the Antiphytoviral composition also wraps
Include：Acceptable auxiliary material composition in Pesticide Science.
6. Antiphytoviral composition according to claim 1, it is characterised in that the Antiphytoviral combination dosage form
For soluble concentrate, solvable pulvis, emulsifiable granula, wettable powder or water dispersible granules.
A kind of 7. application of Antiphytoviral composition for preventing and controlling crop virosis according to any one of claim 1~6.
8. application according to claim 7, it is characterised in that the concentration of the Antiphytoviral composition be 10~
1000mg/Kg。
9. application according to claim 7, it is characterised in that the application process of the Antiphytoviral composition is：In
The crop virosis morbidity early stage or initial stage apply.
10. application according to claim 7, it is characterised in that the application process of the Antiphytoviral composition is to dip in
One or more in root, spraying and pouring root.
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| CN201710978262.9A CN107593709A (en) | 2017-10-18 | 2017-10-18 | A kind of Antiphytoviral composition and its application |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710978262.9A CN107593709A (en) | 2017-10-18 | 2017-10-18 | A kind of Antiphytoviral composition and its application |
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Citations (3)
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|---|---|---|---|---|
| EP0908097A1 (en) * | 1996-11-21 | 1999-04-14 | Plant Biological Defence System Laboratories | Method for preventing or controlling viral diseases of plants |
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| CN104782655A (en) * | 2015-03-24 | 2015-07-22 | 安徽省农业科学院烟草研究所 | Salt-resistive seed soaking liquid of corn and preparation method of salt-resistive seed soaking liquid |
| CN105503415A (en) * | 2015-12-29 | 2016-04-20 | 芜湖润蓝生物科技有限公司 | Efficient micromolecular coated fertilizer beneficial to increasing content of anthocyanin in purple sweet potatoes and preparation method thereof |
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