CN100515170C - method for preparing gentianella seed which can prevent spot blight and method for treating gentianella spot blight - Google Patents
method for preparing gentianella seed which can prevent spot blight and method for treating gentianella spot blight Download PDFInfo
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- CN100515170C CN100515170C CNB2008100640021A CN200810064002A CN100515170C CN 100515170 C CN100515170 C CN 100515170C CN B2008100640021 A CNB2008100640021 A CN B2008100640021A CN 200810064002 A CN200810064002 A CN 200810064002A CN 100515170 C CN100515170 C CN 100515170C
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- leaf blight
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Abstract
The invention relates to a Gentiana seed that can prevent Gentian leaf blight and a preparation method thereof, and a control method for the Gentian leaf blight. The invention relates to a disease preventing Gentiana seed, the preparation method for the Gentiana seed, and a control method for plant diseases, which solves the problems that the Gentian leaf blight at present seriously harms the planting and production of the Gentiana, while the existing control method adopted is of bad effect, large amount of labor, low production efficiency, easy damage of seedlings in the process of operation as well as pesticide residue and environment pollution. The Gentiana seed consists of thiophanate methyl and polyoxin. The preparation method relates to: immersing the seeds in ethanol; then taking the seeds out and dipping in a polyoxin or thiophanate methyl liquid. The control method relates to: dipping the seeds in ethanol; then taking the seeds out and dipping in a diluent reagent liquid; and then rinsing with clear water, namely finishing the control treatment. The incidence rate for the Gentian leaf blight of the seeds of the invention is zero, while the seed germination rate arrives at 54 percent above. The control method for the Gentian leaf blight of the invention is of good effect, with which the rate of mildew can be reduced to zero, while the germination rate can be higher than 51 percent.
Description
Technical field
The present invention relates to a kind of rough gentian seed method of making of diseases prevention and the method for preventing and treating of plant disease.
Background technology
Felwort (Gen tiana scabra Bge.) is the Gentianaceae herbaceos perennial, has purging the liver of pathogenic fire courage excess fire, removes effects such as damp-heat in lower-JIAO; And the gentiopicrin of discovering in the felwort in recent years to be contained has good medical value, so the cultivated area of felwort constantly enlarges.Do not belong to for the maximum disease non-" gentian leaf blight " of felwort plantation harm, occur blue-black haloing around the gentian leaf blight main harm blade, their early stage scab, scab constantly enlarges later on, rounded or oval, diameter is 2～12mm, and the centre is a bronzing, the edge is a dark brown, all there is pore on the scab two sides, and scab often converges mutually when serious, causes the felwort whole blade withered, cause the full wafer plant withered, even total crop failure.
Gentian leaf blight is 100% at the incidence of disease in main growing area Heilungkiang, Liaoning and the Jilin Province of felwort, disease index 25%～95%, and average underproduction amplitude reaches 30%～50%.At present for the output that improves felwort, stop the generation of gentian leaf blight and spread main employing the rough gentian seedling is carried out drug disinfection and transplanting, and two kinds of methods of chemical agent are sprayed in the field.Not only increased the amount of labour, reduced production efficiency with transplanting but for the extensive field planting of rough gentian, seedling is carried out drug disinfection, and in operating process, caused damage easily seedling; The field is sprayed chemical agent and is then had residue of pesticide and environmental pollution problems; And two kinds of methods are all relatively poor to the gentian leaf blight control efficiency, and disease index still is higher than 20%, and can not stop spreading and generation once again of gentian leaf blight fully.
Summary of the invention
The objective of the invention is in order to solve present gentian leaf blight serious harm rough gentian plantation and to produce, and the existing method of the preventing and treating weak effect that adopts, and have that the amount of labour is big, production efficiency is low, easily in operating process, cause damage seedling, and residue of pesticide and environmental pollution problems, and the method for preventing and treating of the rough gentian seed method of making of a kind of anti-gentian leaf blight that provides and gentian leaf blight.
Contain 10～12g thiophanate methyl or 0.6～1g polyoxin in every kilogram in the rough gentian seed of anti-gentian leaf blight.
The preparation of the rough gentian seed of above-mentioned anti-gentian leaf blight is carried out according to the following steps: earlier the rough gentian seed being immersed in mass concentration is 30s in 65%～75% ethanolic solution, take out the polyoxin of putting into 300～500 times of dilutions then or dilute 300～600 times thiophanate methyl solution soaking 30min, dry in the shade after taking out again, promptly obtain the rough gentian seed of anti-gentian leaf blight.
The method of preventing and treating of gentian leaf blight is carried out according to the following steps: earlier the rough gentian seed being immersed in mass concentration is 30s in 65%～75% ethanolic solution, take out then 300～600 times of polyoxin, dilutions putting into 300～500 times of dilutions 400～500 times of thiophanate methyl, dilutions 500～1500 times of ambam, dilutions 5000 times of Shi Jiale, dilutions mancozeb or dilute 1000～2500 times the high solution soaking 30min of generation, take out again and use flushing with clean water 4～5 times, promptly obtain having finished the gentian leaf blight control and handle.
Because the gentian leaf blight pathogen is attached to propagating once more on the seed and infecting, so the rough gentian seed becomes the source of infection of new rough gentian growing area gentian leaf blight, the present invention is from disease source control (the rough gentian seed is handled), gentian leaf blight is just killed it when also being safe from harm blade, can effectively control the propagation of gentian leaf blight, reach the effect of half common multiple of getting over.
The incidence of disease of the rough gentian seed of the anti-gentian leaf blight of the present invention gentian leaf blight in new planting environment is 0, and percentage of seedgermination reaches more than 54%.Simple, the easy operation of preparation method of the rough gentian seed of anti-gentian leaf blight.
The method of preventing and treating of gentian leaf blight of the present invention is effective, and mouldy rate is reduced to 0, and germination rate is higher than 51%.The method of preventing and treating of gentian leaf blight of the present invention is simple to operate, the amount of labour is few, be easy to control, production efficiency height, little to the damage of rough gentian seed, and non agricultural chemical residuum in the rough gentian medicinal material, improved the rough gentian quality of medicinal material, environmental pollution is little, can ignore, and reduced planting cost simultaneously again.
Embodiment
Embodiment one: contain 10～12g thiophanate methyl or 0.6～1g polyoxin in every kilogram in the rough gentian seed of the anti-gentian leaf blight of present embodiment.
The incidence of disease of the rough gentian seed of the anti-gentian leaf blight of present embodiment gentian leaf blight in new planting environment is 0.Germination and growth that thiophanate methyl and polyoxin be harmless to the rough gentian seed, do not influence the rough gentian seed, percentage of seedgermination reaches more than 54%.
Embodiment two: the difference of present embodiment and embodiment one is: contain 11g thiophanate methyl or 0.8g polyoxin in every kilogram in the rough gentian seed of anti-gentian leaf blight.
Embodiment three: the rough gentian seed of the anti-gentian leaf blight of present embodiment carries out according to the following steps: earlier the rough gentian seed being immersed in mass concentration is 30s in 65%～75% ethanolic solution, take out the polyoxin of putting into 300～500 times of dilutions then or dilute 300～600 times thiophanate methyl solution soaking 30min, dry in the shade after taking out again, promptly obtain the rough gentian seed of anti-gentian leaf blight.
The used thiophanate methyl of present embodiment is a wetting powder, and medicament proper mass concentration is 65%～75%.
The used polyoxin of present embodiment is a wetting powder, and medicament proper mass concentration is 2%～4%.
Embodiment four: the difference of present embodiment and embodiment three is: the proper mass concentration of thiophanate methyl wetting powder is 70%; The proper mass concentration of polyoxin wetting powder is 3%.Other step and parameter are identical with embodiment three.
Embodiment five: the difference of present embodiment and embodiment three is: earlier the rough gentian seed being immersed in mass concentration is 30s in 70% ethanolic solution.Other step and parameter are identical with embodiment three.
Embodiment six: the difference of present embodiment and embodiment three is: put into the polyoxin of 400 times of dilutions or dilute 400～500 times thiophanate methyl solution soaking 30min after taking out from ethanol.Other step and parameter are identical with embodiment three.
Embodiment seven: the method for preventing and treating of present embodiment gentian leaf blight is carried out according to the following steps: earlier the rough gentian seed being immersed in mass concentration is 30s in 65%～75% ethanolic solution, take out then 300～600 times of polyoxin, dilutions putting into 300～500 times of dilutions 400～500 times of thiophanate methyl, dilutions 500～1500 times of ambam, dilutions 5000 times of Shi Jiale, dilutions mancozeb or dilute 1000～2500 times the high solution soaking 30min of generation, take out again and use flushing with clean water 4～5 times, promptly obtain having finished the gentian leaf blight control and handle.
In the present embodiment rough gentian seed soak not only earlier with ethanol can sterilization, sterilization, simultaneously can reduce the surface tension of water at the seed epidermis, antibiotic, bactericidal agent (polyoxin, thiophanate methyl, ambam, Shi Jiale or generation height) of later stage can fully be contacted with rough gentian seed epidermis, reach the purpose of thorough sterilization, sterilization.
The used thiophanate methyl of present embodiment is a wetting powder, and medicament proper mass concentration is 65%～75%.
The used polyoxin of present embodiment is a wetting powder, and medicament proper mass concentration is 2%～4%.
Ambam (Amobam; Dithanestainless; Ammonium ethylene bis-dithio carbamate) have another name called A Muba, ethylene bisdithiocarbamic ammonium, pure product are colourless crystallization, and former medicine is the orange-yellow or faint yellow aqueous solution, is alkalescent, and ammonia and hydrogen sulphide stink are arranged.The used ambam of present embodiment is an aqua, and medicament proper mass concentration is 40%～50%.
Shi Jiale (phonetic mould amine) outward appearance is the white crystals powder, and no special odor is almost tasteless.Be difficult for decomposing, nonflammable, non-explosive, non-corrosiveness can reach 3 years at normal temperatures storage period, belongs to the low toxicity bactericide.The used Shi Jiale original content of present embodiment is 300～500 grams per liters.
The used mancozeb of present embodiment is a wetting powder, and medicament proper mass concentration is 65%～75%.
The generation height has another name called mute ether azoles, opposes the pellet that withers, safety and low toxicity wide-spectrum bactericide.The used generation height of present embodiment is the agent of water shot, and medicament proper mass concentration is 8%～12%.
The used happy generation height of polyoxin, thiophanate methyl, ambam, Shi Jia of present embodiment all is low toxicity bactericide, the agricultural chemicals little to environmental hazard.
Comparative trial:
Press the treatment conditions of record in the table 1 and handle the rough gentian seed, be placed on then on the filter paper of sterilizing in the culture dish and (be equipped with the absorbent cotton of sterilization under the filter paper, remain absorbent cotton in the process of the test and contain aqua sterilisa) in preserve moisture, heat insulating culture, cultivate after the 12nd day and the 30th day germination rate and the mouldy rate (result of the test is as shown in table 1) measured.
Table 1
Select the thiophanate methyl wetting powder available from the triumphant Jiang Nonghua Co., Ltd in Jiangyin in this experiment for use, medicament proper mass concentration is 70%; Select for use available from two polyoxin wetting powders of receiving chemical industry Co., Ltd in Shanghai, medicament proper mass concentration is 3%; Select for use available from the ambam aqua of LiMin chemical engineering Co.,Ltd, medicament proper mass concentration is 45%; Select for use available from the Shi Jiale of Bayer Cropscience Co., Ltd Beijing Company, original content is 400 grams per liters; Select for use available from the mancozeb wettable powder of LiMin chemical engineering Co.,Ltd, medicament proper mass concentration is 70%; Select for use available from the high water shot of the generation agent that is just reaching earlier crop protection Co., Ltd in Switzerland, medicament proper mass concentration is 10%; Select for use available from the mould Lingshui Spring agent of evil of Binzhou nine Anhua worker Co., Ltds, medicament proper mass concentration is 30%.
Experimental data (method of preventing and treating of present embodiment gentian leaf blight) according to table 1 was cultivated not grow mold in 30 days, showed that the method for preventing and treating of present embodiment gentian leaf blight can be killed the entrained gentian leaf blight of seed, avoided the generation of gentian leaf blight.The experimental data of table 1 also illustrates not to be that all antibiotic, bactericidal agents all are fit to present embodiment method, for example Wakil; And concentration antibiotic, bactericidal agent is not high more good more yet, has only the concentration of proper drug to guarantee higher germination rate when guaranteeing to reduce mouldy rate.
Embodiment eight: the difference of present embodiment and embodiment seven is: each time of flushing with clean water is 10～15min.Other step and parameter are identical with embodiment seven.
Embodiment nine: the difference of present embodiment and embodiment seven is: earlier the rough gentian seed being immersed in mass concentration is 30s in 70% ethanolic solution.Other step and parameter are identical with embodiment seven.
Embodiment ten: the difference of present embodiment and embodiment seven is: earlier the rough gentian seed being immersed in mass concentration is 30s in 75% ethanolic solution.Other step and parameter are identical with embodiment seven.
Embodiment 11: the method for preventing and treating of present embodiment gentian leaf blight is carried out according to the following steps: earlier the rough gentian seed being immersed in mass concentration is 30s in 70% ethanolic solution, take out the Shi Jiale solution soaking 30min that puts into 1000 times of dilutions then, take out again and use flushing with clean water 4～5 times, promptly obtain preventing and treating the rough gentian seed of processing.
Embodiment 12: the method for preventing and treating of present embodiment gentian leaf blight is carried out according to the following steps: earlier the rough gentian seed being immersed in mass concentration is 30s in 75% ethanolic solution, take out the thiophanate methyl liquid of putting into 500 times of dilutions then and soak 30min, take out again and use flushing with clean water 5 times, promptly obtain preventing and treating the rough gentian seed of processing.
The proper mass concentration of present embodiment thiophanate methyl wetting powder is 70%, cultivates and does not have fungus growth after 30 days, and the rough gentian percentage of seedgermination is 73%.
Embodiment 13: the difference of present embodiment and embodiment 12 is: take out the thiophanate methyl liquid of putting into 400 times of dilutions after being immersed in the rough gentian seed in the ethanolic solution and soak 30min.Other step and parameter are identical with embodiment 12.
Present embodiment was cultivated after 30 days does not have fungus growth, and the rough gentian percentage of seedgermination is 72%.
Claims (3)
1, the rough gentian seed method of making of anti-gentian leaf blight, the preparation that it is characterized in that the rough gentian seed of anti-gentian leaf blight is carried out according to the following steps: earlier the rough gentian seed being immersed in mass concentration is 30s in 65%～75% ethanolic solution, take out the polyoxin of putting into 300～500 times of dilutions then or dilute 300～600 times thiophanate methyl solution soaking 30min, dry in the shade after taking out again, promptly obtain the rough gentian seed of anti-gentian leaf blight.
2, the method of preventing and treating of gentian leaf blight, the method of preventing and treating that it is characterized in that gentian leaf blight is carried out according to the following steps: earlier the rough gentian seed being immersed in mass concentration is 30s in 65%～75% ethanolic solution, take out the polyoxin of putting into 300～500 times of dilutions then, dilute 300～600 times thiophanate methyl, dilute 400～500 times ambam, dilute 500～1500 times Shi Jiale, dilute 5000 times mancozeb or dilute 1000～2500 times the high solution soaking 30min of generation, take out again and use flushing with clean water 4～5 times, promptly obtain having finished the gentian leaf blight control and handle.
3, the method for preventing and treating of gentian leaf blight according to claim 2 is characterized in that each time of flushing with clean water is 10～15min.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2008100640021A CN100515170C (en) | 2008-02-15 | 2008-02-15 | method for preparing gentianella seed which can prevent spot blight and method for treating gentianella spot blight |
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| CNB2008100640021A CN100515170C (en) | 2008-02-15 | 2008-02-15 | method for preparing gentianella seed which can prevent spot blight and method for treating gentianella spot blight |
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| CN101228818A CN101228818A (en) | 2008-07-30 |
| CN100515170C true CN100515170C (en) | 2009-07-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627689B (en) * | 2009-08-03 | 2011-01-12 | 云南省农业科学院药用植物研究所 | Control method of gentiana rigescens spot blight |
-
2008
- 2008-02-15 CN CNB2008100640021A patent/CN100515170C/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
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| 龙胆斑枯病的化学防治研究. 孙海峰等.中医药信息，第21卷第3期. 2004 |
| 龙胆斑枯病的化学防治研究. 孙海峰等.中医药信息，第21卷第3期. 2004 * |
| 龙胆草斑枯病的综合防治. 秦佳梅等.特种经济动植物，第3期. 1999 |
| 龙胆草斑枯病的综合防治. 秦佳梅等.特种经济动植物，第3期. 1999 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627689B (en) * | 2009-08-03 | 2011-01-12 | 云南省农业科学院药用植物研究所 | Control method of gentiana rigescens spot blight |
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| CN101228818A (en) | 2008-07-30 |
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