CN107586748A - A kind of Chinese sporangium and its application - Google Patents
A kind of Chinese sporangium and its application Download PDFInfo
- Publication number
- CN107586748A CN107586748A CN201711041284.9A CN201711041284A CN107586748A CN 107586748 A CN107586748 A CN 107586748A CN 201711041284 A CN201711041284 A CN 201711041284A CN 107586748 A CN107586748 A CN 107586748A
- Authority
- CN
- China
- Prior art keywords
- sporangium
- chinese
- neau
- sinomonas
- bacterial strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of Chinese sporangium and its application, and the invention belongs to microbiological art.Solve promotion growth inapparent technical problem of the existing Chinese sporangium to plant.Chinese sporangium provided by the invention, bacterial strain is Sinomonas sp.NEAU HV1, China typical culture collection center (abbreviation CCTCC) is preserved on May 18th, 2017, preservation place is Wuhan City, Hubei Province Wuhan University, and preserving number is CCTCC NO：M2017246, the bacterial strain can be applied to promote plant growth, significant effect in the form of composition such as microbial inoculum or bio-feritlizer or in the form of bacterium solution.
Description
Technical field
The invention belongs to microbiological art, and in particular to a kind of new Chinese sporangium and its application.
Background technology
Chemical fertilizer plays an important role in China's agricultural production, but its long-term a large amount of or inappropriate use can cause
The heavy metal pollution of soil, the shortage of soil with organic matter and humus, soil texture destruction, soil hardening are barren, also result in
Harmful element is accumulated, and nitrate content is exceeded in vegetable and fruit, and quality of agricultural product declines, and environmental and human health impacts are caused not
Profit influences.Many microorganisms provide required nutriment for plant growth by its physiological activity in soil, increase crop
Yield, improve crop quality and improve resistance of the plant to pathogen.Therefore, developed using beneficial microbe in soil into micro- life
Thing fertilizer can be as chemical fertilizer supplement or potential substitute, avoiding the pollution of chemical fertilizer and to soil fail
Meanwhile the yield and quality of crop can be improved.
Plant growth-promoting rhizobacteria (plant growth-promoting rhizobacteria, abbreviation PGPR) is to be lodged in
The microorganism of plant rhizosphere, root table, there is fixed nitrogen, dissolved metals or organophosphor, produce the plant hormones such as auxin, produce it
It can promote rhizosphere bacteria one or more in the abilities such as active material and the secretion antibiotic of plant growth, their energy
The growth and development of enough direct or indirect promotion plants.PGPR can increase fresh weight and dry weight, the promotion plant root life of plant
Long and plant height increase, increase leaf area and chlorophyll content, the nitrogen content for improving plant, increase can tiller plant tiller number,
Improve the yield of crop and the sprouting of seed can be promoted.Having now found that the rhizosphere bacteria of more than 20 category kind has PGPR growth-promoting special
Property, including pseudomonas (Pseudomonas), bacillus (Bacillus), Agrobacterium (Agrobacterium),
Erwinia (Eriwinia), Flavobacterium (Flavobacterium), Serratia (Serratia), Enterobacter
(Enterobacter) and Klebsiella (Klebsiella) etc., wherein with pseudomonas and bacillus
Effect is preferable, and is reported at most.
Chinese sporangium (Sinomonas sp.) is micrococcaceae (Micrococcaceae) gram-positive bacteria, bacterium
Volume morphing is shaft-like, arcuation or the growth circulation of bar-ball is presented, and main cell hydrolysis sugar is galactolipin, mannose and ribose, carefully
The peptide bridge of cell wall peptide glycan is A3 α types, and main methylnaphthoquinone is MK-9 (H2), main phosphate lipid is that double phosphatidyls are sweet
Oil, phosphatidyl glycerol and phosphatidylinositols, main fatty acid iso-C15:0、anteiso-C15:0And anteiso-C17:0.China
Sporangium was set up as the new category of micrococcaceae in 2009, and its type culture is yellow matter China sporangium Sinomonas
Flava, 10 plants of Chinese sporangiums reflect from the environment such as soil, plant rhizosphere soil and volcanic rock surface are separated so far
It is fixed, but be rarely reported on the biological function research of Chinese sporangium.2014, the isolated one plant of production of Cai Jun et al. was yellow
The Chinese sporangium Sinomonas sp.HSD8 of aspertoxin B1 digestive enzymes,；2016, the equal seminars of Zhongshan University Li Wen were first
Secondary discovery Sinomonas mesophila have the ability of synthesis silver nano-grain, and it is resistance to that the nano particle can effectively suppress multiple medicine
The growth of property of medicine staphylococcus aureus；The same year, Japanese University of tsukuba researcher isolated one plant of sesamin from soil
Degradation bacteria strains Sinomonas sp.No.22, and clone identification goes out first microbe-derived sesamin degraded from the bacterial strain
Enzyme；2017, research group of South Korea, which is separated to one plant while had from plant rhizosphere soil, promoted watermelon growing and preventing and treating west
The Chinese sporangium Sinomonas atrocyanea NSB-27 of melon bacterial fruit blotch disease.Both at home and abroad on Chinese sporangium
Research as PGPR bacterial strains is still in the starting stage, and the growth-promoting effect of strains on plant is not notable.
The content of the invention
To solve the above problems, the present invention provides a kind of Chinese sporangium and its application.
Bacterial strain Sinomonas sp.NEAU-HV1 provided by the invention are preserved in Chinese Typical Representative on May 18th, 2017
Culture collection (abbreviation CCTCC), preservation place are Wuhan City, Hubei Province Wuhan University, and preserving number is CCTCC NO：
M2017246。
Sinomonas sp.NEAU-HV1 are Gram-positive, and obvious shaft-like and spherical cycle change is presented in thalline
Change, atrichia, do not move, 28 DEG C of optimum growth temperature, optimal pH 7.0, its single bacterium colony is in NA culture mediums (beef extract 5g/L, egg
White peptone 10g/L, sodium chloride 5g/L, agar 15g/L) on be yellow, glossy, flat, rounded, surface and the smooth of the edge, side
Edge is neat, moistens and sticks greasy, easy picking；Positive and negative, edge and central part solid colour.Bacterial strain Sinomonas sp.NEAU-
HV1 contacts enzyme positive, MR and VP negatives, Starch Hydrolysis is positive, gelatin liquefaction positive, and nitrate reduction is positive, citric acid
Utilize feminine gender.
The present invention also provides Chinese sporangium Sinomonas sp.NEAU-HV1 and is preparing the composition of promotion plant growth
In application.
The composition is microbial inoculum or bio-feritlizer containing Chinese sporangium Sinomonas sp.NEAU-HV1.
Other Chinese sporangium Sinomonas sp.NEAU-HV1 provided by the invention, which can also be directly used in, promotes plant
Growth.
Beneficial effect：
There is Chinese sporangium Sinomonas sp.NEAU-HV1 provided by the invention significant promotion to make to plant growth
With can promote growth and the output increased of crop, in greenhouse, wheat root elongation is increased by 39%, the increase of root fresh weight amount
146.6%, seedling fresh weight amount increase by 68.4%, root dry weight amount increase by 142.1%, seedling stem weight increase by 52.9%, plant height increase
20.6%；Make the root elongation of corn increase by 43.5%, root fresh weight amount increase by 38.5%, seedling fresh weight amount increase by 114.3%, root is done
Weight increase by 36.5%, seedling stem weight increase by 92.5%, plant height increase by 22.5%；Make the leaf area of romaine lettuce increase by 99.2%, ground
Upper part fresh weight increase by 117.4%.Bio-feritlizer containing above-mentioned Chinese sporangium to cucumber, tomato, soybean, carnation,
The effect of being significantly improved of the yield of vine fruit or petal, output increased is up to 5-27% (quality).It can be seen that the present invention carries
The Chinese sporangium Sinomonas sp.NEAU-HV1 supplied are respectively provided with significantly to crop, veterinary antibiotics, flowers or nursery stock etc.
Promote growth.Bacterial strain Sinomonas sp.NEAU-HV1 and its microbial inoculum provided by the invention are pollution-free in use,
It is nuisanceless, the dosage of chemical fertilizer can be reduced, can be widely applied to plant growth-promoting field, there is preferable economic benefit and society
Benefit.
Brief description of the drawings：
The 16S rDNA sequential systems that Fig. 1 is bacterial strain NEAU-HV1 develop status.
Fig. 2 is growth-promoting design sketch of the bacterial strain NEAU-HV1 to wheat.
Fig. 3 is growth-promoting design sketch of the bacterial strain NEAU-HV1 to greenhouse pot culture wheat.
Fig. 4 is growth-promoting design sketch of the bacterial strain NEAU-HV1 to corn.
Fig. 5 is growth-promoting design sketch of the bacterial strain NEAU-HV1 to greenhouse pot culture corn.
Fig. 6 is growth-promoting design sketch of the bacterial strain NEAU-HV1 to greenhouse pot culture romaine lettuce.
Embodiment
Below by way of specific embodiment, the invention will be further described, but not to limit the present invention, it is all in the present invention
Within spirit and principle, any modification, equivalent substitution and improvements done etc., it should be included in the scope of the protection.
The Chinese sporangium Sinomonas sp.NEAU-HV1 of embodiment 1 culture and identification
1. the separation and culture of bacterial strain
Soil sample is collected in the torrid areas (13 ° of 0 ' N, 15 ° of 44 ' E) of Republic of Chad Ha Jieer-La Mi provinces, by 5g soil
Sample is dissolved into the triangular flask of 45ml sterile salines (band bead), is vibrated 30 minutes under 180rpm rotating speeds, stands 10
After minute, after taking supernatant liquid to dilute 10 times, the dilution for drawing 0.5ml is coated on NA plating mediums, is placed in 28 DEG C
Culture 2 days, picking single bacterium colony is inoculated into fresh NA plating mediums, through transferring to obtain purifying bacterial strain repeatedly, by the bacterial strain of purifying
NEAU-HV1 is named as, is stored in 20% glycerine in -80 DEG C of ultra low temperature freezers.
2. the identification of bacterial strain
Authentication method：Pass through morphological feature, bio-chemical characteristics, cytochemistry component and 16S rDNA sequence analyses pair
The bacterial strain is identified.
Morphological features：Gram-positive, there is obvious shaft-like and spherical week in bacterial strain NEAU-HV1 incubations
Phase changes.
Physiological and biochemical property：It is strict aerobic, its gelatin hydrolysis, aesculin hydrolysis, cellulose decomposition, urase, VP experiments, lemon
The utilization of lemon hydrochlorate is positive, and H2S generations, tween hydrolysis, Starch Hydrolysis, methyl red test are negative (being shown in Table 1)；Cotton seed can be utilized
Sugar, sucrose, rhamnose, ribose, arabinose, xylose, maltose, lactose, mannose, fructose, galactolipin, sorbose and indoles
The carbon sources such as acetic acid, it is impossible to utilize the carbon source such as mannitol, inositol, glucose, sweet and pure；Aspartic acid, tyrosine, dried meat ammonia can be utilized
The nitrogen sources such as acid, glutamine, serine, asparagine, glutamic acid, arginine, threonine, alanine, it is impossible to utilize glycine.
Table 1NEAU-HV1 is compared with the physio-biochemical characteristics of similar strain
Note："+" is the positive, and "-" is feminine gender.
Cytochemistry component analysis：
The main Types of methylnaphthoquinone are MK-8 (H2)、MK-9(H2) and MK-10 (H2), phosphate lipid main Types are
Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and glycolipid,
Full cell hydrolysis sugar includes galactolipin, rhamnose and mannose, fatty acid species and content such as table 2.
The aliphatic acid of the bacterial strain HV1 of table 2 and its similar strain forms and content
Note："-" is feminine gender.
16S rDNA gene magnifications and sequence analysis：
Chinese sporangium NEAU-HV1 28 DEG C of cultures in NA culture mediums to logarithmic phase, are received with 12000r/min centrifugations 5min
Collect thalline, using the bacterial genomes DNA extraction kit of Tiangeng biochemical technology Co., Ltd, extract the genomic DNA of bacterium,
Using the DNA product of extraction as template, universal primer 27F (5 '-AGAGTTTGATCCTGGCTCAG- are expanded with bacterial 16 S rDNA
3 ') and 1541R (5 '-AAGGAGGTGATCCAGCC-3 ') amplifies 16S rDNA genetic fragments from genomic DNA.PCR is produced
Thing send Jilin bio tech ltd of provincial treasury U.S. to be sequenced.16SrDNA sequences such as SEQ ID NO:Shown in 1, the sequence is
Log in NCBI, accession number MF418645.Sequence alignment analysis is carried out in EzBioCloud by blast program, and used
NEGA 4.0 builds bacterial strain phylogenetic tree.As a result the 16S rDNA sequences of the bacterial strain and the Chinese sporangium delivered at present are shown
Sinomonas susongensis A31TSimilitude is less than 99% up to 99.24%, with the similitude of other Chinese sporangiums.
Comprehensive thalli morphology, physio-biochemical characteristics, cellular component and 16S rDNA gene orders, the bacterial strain of separation is identified
For Sinomonas sp.NEAU-HV1, phylogenetic tree is shown in Fig. 1.The bacterial strain is preserved in Chinese Typical Representative training on May 18th, 2017
Thing collection (abbreviation CCTCC) is supported, preservation place is Wuhan City, Hubei Province Wuhan University, and preserving number is CCTCC NO：
M2017246。
16S rDNA sequences
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGGACGATGAAGCC
CTTCGGGGTGGATTAGTGGCGAACGGGTGAGTAACACGTGAGCAACCTGCCCTTGACTCTGGGATAAGCCTGGGAAG
CTGGGTCTAATACCGGATACATCCTCCTGCCGCATGGTGGGGGGTGGAAAGCCTTGTGCGGTCTTGGATGGGCTCGC
GGCCTATCAGCTTGTTGGTGGGGTGATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCAC
ACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATG
CAGCGACGCCGCGTGGGGGATGACGGCCTTCGGGTTGTAAACCCCTTTCGGTGGGGAACAAGCCGCTTCGGTGGTGA
GGGTACCCGCGGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTATCCGGA
ATTATTGGGCGTAAAGAGCTCGTAGGCGGTTTGTCGCGTCTGCTGTGAAAGCCCGGGGCTCAACCCCGGGTCTGCAG
TGGGTACGGGCAGGCTGGAGTGCAGTAGGGGAGACTGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAG
GAACACCGATGGCGAAGGCAGGTCTCTGGGCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTA
GATACCCTGGTAGTCCACGCCGTAAACGTTGGGCACTAGGTGTGGGGGGCATTCCACGTCCTCCGCGCCGCAGCTAA
CGCATTAAGTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGG
CGGAGCATGCGGATTAATTCGATGCAACGCGAAGAACCTTACCAAGGCTTGACATGGGCCGGACCGCTCCAGAGATG
GGGCTTCCCTTCGGGGCCGGTTCACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCC
CGCAACGAGCGCAACCCTCGTTCCATGTTGCCAGCACTTCGGGTGGGGACTCATGGGAGACTGCCGGGGTCAACTCG
GAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGCCTTGGGCTTCACGCATGCTACAATGGCCGGTACAA
AGGGTTGCGATGCTGTGAGGTTGAGCCAATCCCAGAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCC
ATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCAACGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCC
GTCAAGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTTGTGGGGGGGAGCCGTCGAAGGTGGGACT
GGCGATTGGGACTAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATCACCTCCTT
The Chinese sporangium Sinomonas sp.NEAU-HV1 of embodiment 2 are tested the growth-promoting of wheat
1. the vernalization experiment of wheat：
By inoculation in TSB fluid nutrient mediums (pancreas peptone soybean broth 3g/100ml), 28 degree of 250rpm shake
Culture is swung, fresh TSB medium cultures 24h is then inoculated into 1% inoculum concentration, collects thalline, after PBS washs,
1.0 × 10 are diluted to respectively5-1.0×109Cfu/ml concentration is standby.Select that full wheat seed is some to be placed on 1- in running water
The shrivelled seed of floating is weeded out within 2 minutes, then with 70% alcohol is sterilized to wheat seed 3-5 minutes successively, sterilized water is clear
Wash 3-5 times, 5% liquor natrii hypochloritis sterilizes 3-5 minutes and sterile water wash 3-5 times.By cultured Sinomonas sp.
NEAU-HV1 is diluted to 10 with sterilized water5、106、107、108With 109Cfu/ml, the wheat seed that then will be handled well
It is put into immersion 8-12 hours to wheat in the bacteria suspension of concentrations above to show money or valuables one carries unintentionally, control group (CK) is carried out with the sterilized water of equal volume
Immersion.The wheat to show money or valuables one carries unintentionally is gently pressed from both sides out with tweezers, is placed in the plate equipped with double-layer filter paper and (is added on each plate filter paper
The bacterium solution suspension of 5ml concentrations above, it is positioned over 28 DEG C, cultivates in the growth cabinet of humidity 70%.The root of wheat is measured after 7 days
Long and bud length.As a result Fig. 2 and table 3 are seen.Compared with the control, germinations of the bacterial strain Sinomonas sp.NEAU-HV1 to wheat has
Facilitation, when the concentration of bacterium solution is 107During cfu/ml, growth-promoting effect is most obvious, and bud length and root long increase by 32.2% He respectively
36%.
Table 3 is inoculated with influence of the bacterial strain NEAU-HV1 bacterium solutions of various concentrations to wheat growth-promoting
2. wheat potted plant experiment：
Prepare mycelium dilution solution according to above method, and handle wheat seed, by the seed to show money or valuables one carries unintentionally it is orderly put
In the basin for filling 1kg soil, 30 seeds are placed per basin, each processing sets 3 repetitions, using the PBS of not mycetome as
Compare (CK).After 30 days, the plant height of wheat, root long, Miao Hegen fresh weight and dry weight are measured respectively.As a result Fig. 3 and table 4 are seen.With
Control is compared, when the concentration of bacterial strain is 1.0 × 108During cfu/ml, growth-promoting effect is most obvious, and plant height increase by 20.6%, root long increases
Add 26.7%, seedling fresh weight increase by 68.4%, seedling stem increases by 52.9% again, and root fresh weight increase by 146.7%, root dry weight increases by 60%.
Table 4 is inoculated with influence of the bacterial strain NEAU-HV1 bacterium solutions of various concentrations to potted plant wheat growth-promoting
The Chinese sporangium Sinomonas sp.NEAU-HV1 of embodiment 3 are tested the growth-promoting of corn
1. the vernalization experiment of corn：
By inoculation in NA fluid nutrient mediums (specific composition), 28 degree of 250rpm shaken cultivations, then connect with 1%
Kind of amount is inoculated into fresh TSB medium cultures 24h, collects thalline, after PBS washs, it is diluted to 1.0 respectively ×
107cfu/ml-1.0×108Cfu/ml concentration is standby.Select that full corn seed is some to be placed on 1-2 minutes in running water and reject
Fall the shrivelled seed of floating, then successively with 70% alcohol to wheat seed sterilize 3-5 minutes, sterile water wash 3-5 times,
5% liquor natrii hypochloritis sterilizes 3-5 minutes and sterile water wash 3-5 times.By cultured Sinomonas sp.NEAU-HV1
10 are diluted to sterilized water5、106、107、108With 109Cfu/ml, then the corn seed handled well is put into the bacterium of concentrations above
Soak 8-12 hours to wheat in suspension to show money or valuables one carries unintentionally, control group (CK) is soaked with the sterilized water of equal volume.The jade that will be showed money or valuables one carries unintentionally
Rice seed is gently pressed from both sides out with tweezers, is placed in the plate equipped with double-layer filter paper and (5ml concentrations above is added on each plate filter paper
Bacterium solution suspension, it is positioned over 28 DEG C, cultivates in the growth cabinet of humidity 70%.The root long and bud length of corn are measured after 7 days.As a result
See Fig. 4 and table 5.Compared with the control, germinations of the bacterial strain Sinomonas sp.NEAU-HV1 to corn has facilitation, works as bacterium
The concentration of liquid is 1.0 × 108During cfu/ml, growth-promoting effect is most obvious, and bud length and root long increase by 217.8% and 57.5% respectively.
Table 5 is inoculated with influence of the bacterial strain NEAU-HV1 bacterium solutions of various concentrations to corn growth-promoting
2. corn potted plant experiment：
Prepare mycelium dilution solution according to above method, and handle corn seed, by the seed to show money or valuables one carries unintentionally it is orderly put
In the basin for filling 1kg soil, 30 seeds are placed per basin, each processing sets 3 repetitions, using the PBS of not mycetome as
Compare (CK).After 30 days, the plant height of corn, root long, Miao Hegen dry weight and fresh weight are measured respectively.As a result Fig. 5 and table 6 are seen.With
Control is compared, when the concentration of bacterial strain is 1.0 × 108During cfu/m, growth-promoting effect is most obvious, and plant height increases by 22.5%, root long increase
45.4%, seedling fresh weight increase by 114.3%, seedling stem increases by 36.2% again, and root fresh weight increase by 92.5%, root dry weight increases by 14.3%.
Table 6 is inoculated with influence of the bacterial strain NEAU-HV1 bacterium solutions of various concentrations to potted plant corn growth-promoting
The Chinese sporangium Sinomonas sp.NEAU-HV1 of embodiment 4 are tested the potted plant growth-promoting of romaine lettuce
Romaine lettuce seed is with after 2-4 hour (being moistened to seed, shorten the germinating time of romaine lettuce) of distilled water immersion
Plant in seedlings nursing plate, after the time of about two weeks, select the consistent romaine lettuce of growing way and transplanted, by cultured Sinomonas
Sp.NEAU-HV1 is diluted to 10 with sterilized water5、106、107、108With 109Cfu/ml is directly poured onto the root of romaine lettuce, control group
Pour isometric distilled water.Romaine lettuce is surveyed after 28 days and produced, as a result sees Fig. 6 and table 7.Compared with compareing (CK), when the concentration of bacterium solution
For 106~107During cfu/ml, growth-promoting effect is most obvious, and leaf area and overground part fresh weight increase by 99.2% and 117.4% respectively
Table 7 is inoculated with influence of the bacterial strain NEAU-HV1 bacterium solutions of various concentrations to potted plant romaine lettuce growth-promoting
Embodiment 4
Bacterial strain Sinomonas sp.NEAU-HV1 are inoculated into the (pancreas peptone soybean broth in TSB fluid nutrient mediums
3g/100ml), 28 DEG C of 250rpm shaken cultivations, fresh TSB culture mediums kind culture is then inoculated into 1% (v/v) inoculum concentration.Press
According to mass ratio 1:10-14 ratio, above-mentioned zymotic fluid is drenched in solid medium, add appropriate deionized water, make water
Point content reaches 20-40%, then stirs and evenly mixs, and is fitted into fermentation tank, and natural packing ferments 4-5 days, during which keeps moisture
Content is 20-40%, and is passed through filtrated air and keeps fermentation tank internal temperature to be less than 40 DEG C；After fermentation ends, spontaneously dry extremely
Moisture is less than 5%, obtains fermenting agent.Wherein solid medium is made as follows：By part cotton dregs of 3 weight, 4
Parts by weight peanut shell powder, 2 parts by weight wheat bran, 0.2 parts by weight molasses, 2 parts by weight peanut cakes, 2.5 parts by weight dry silkworm excrements, 10 weight
Bagasse, 0.006 weight that the dry corn stalk powder of part, 0.8 parts by weight plant ash, 2 parts by weight fishbone dusts, 4 parts by weight are dried
Part cellulase, 0.8 parts by weight Dry chick faeces, 0.1 parts by weight ammonium chloride are measured, crushed 50 mesh sieves after well mixed, then
130-140 DEG C of high-temperature vapor fully sterilizes, that is, obtains the solid medium.
According to 1:20-50 mass ratio, by described Chinese sporangium Sinomonas sp.NEAU-HV1 zymotic fluid or
Fermenting agent is sufficiently mixed with organic matter mixture, and carries out compost fermentation processing, and the China is obtained after post processing
Sporangium Sinomonas sp.NEAU-HV1 microbial manures.Wherein, the organic matter mixture can be used to prepare to be all
The arbitrary proportion mixture of any organic matter of machine fertilizer, such as can be to dry maize straw, drying wheat stalk, wheat bran, drying
Appoint in bagasse, vinasse, dregs of beans, cotton dregs, peanut shell powder, dry rice straw, dry pig manure, Dry chick faeces, various bone meal etc.
Anticipate a variety of mixtures with arbitrary proportion, but in order to which nutrition is comprehensive, preferably wherein at least four kinds of, and preferably add appropriate
The assisted fermentations such as corn flour, beancake powder, groundnut meal, molasses, cellulase, amylase and decomposition nutrition matter.Wherein, it is described
After the completion of post processing refers to compost fermentation, it is dried to moisture and is less than 10%, that is, obtains described bio-feritlizer.
Embodiment 5
Chinese sporangium Sinomonas sp.NEAU-HV1 bio-feritlizers prepared by above-described embodiment 4 are applied before site preparation
With and turn over into soil.Application test sets 2 processing altogether：Processing 1 is that mu applies bio-feritlizer 200kg, and processing 2 is not do any place
The control of reason.Test plant is cucumber, tomato, soybean, carnation, vine.In harvest time, the fruit of test plant is gathered
Or petal, and survey its weight.As a result show, compared with the control, using the micro- lifes of Chinese sporangium Sinomonas sp.NEAU-HV1
After thing fertilizer, fruit maturation or advance flowering period, and the yield of fruit or petal is significantly improved, output increased is up to 5-27%
(weight).Research shows that Chinese sporangium Sinomonas sp.NEAU-HV1 microbial manures are to crop, vegetables, water above
Fruit, flowers or nursery stock etc. are respectively provided with significant promotion growth.
SEQUENCE LISTING
<110>Northeast Agricultural University
<120>A kind of Chinese sporangium and its application
<130>
<160> 3
<170> PatentIn version 3.5
<210> 1
<211> 20
<212> DNA
<213> 27F
<400> 1
agagtttgat cctggctcag 20
<210> 2
<211> 17
<212> DNA
<213> 1541R
<400> 2
aaggaggtga tccagcc 17
<210> 3
<211> 1518
<212> DNA
<213> 16S rDNA
<400> 3
agagtttgat cctggctcag gatgaacgct ggcggcgtgc ttaacacatg caagtcggac 60
gatgaagccc ttcggggtgg attagtggcg aacgggtgag taacacgtga gcaacctgcc 120
cttgactctg ggataagcct gggaagctgg gtctaatacc ggatacatcc tcctgccgca 180
tggtgggggg tggaaagcct tgtgcggtct tggatgggct cgcggcctat cagcttgttg 240
gtggggtgat ggcctaccaa ggcgacgacg ggtagccggc ctgagagggt gaccggccac 300
actgggactg agacacggcc cagactccta cgggaggcag cagtggggaa tattgcacaa 360
tgggcgcaag cctgatgcag cgacgccgcg tgggggatga cggccttcgg gttgtaaacc 420
cctttcggtg gggaacaagc cgcttcggtg gtgagggtac ccgcggaaga agcgccggct 480
aactacgtgc cagcagccgc ggtaatacgt agggcgcgag cgttatccgg aattattggg 540
cgtaaagagc tcgtaggcgg tttgtcgcgt ctgctgtgaa agcccggggc tcaaccccgg 600
gtctgcagtg ggtacgggca ggctggagtg cagtagggga gactggaatt cctggtgtag 660
cggtgaaatg cgcagatatc aggaggaaca ccgatggcga aggcaggtct ctgggctgta 720
actgacgctg aggagcgaaa gcgtggggag cgaacaggat tagataccct ggtagtccac 780
gccgtaaacg ttgggcacta ggtgtggggg gcattccacg tcctccgcgc cgcagctaac 840
gcattaagtg ccccgcctgg ggagtacggc cgcaaggcta aaactcaaag gaattgacgg 900
gggcccgcac aagcggcgga gcatgcggat taattcgatg caacgcgaag aaccttacca 960
aggcttgaca tgggccggac cgctccagag atggggcttc ccttcggggc cggttcacag 1020
gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc 1080
gcaaccctcg ttccatgttg ccagcacttc gggtggggac tcatgggaga ctgccggggt 1140
caactcggag gaaggtgggg acgacgtcaa atcatcatgc cccttatgcc ttgggcttca 1200
cgcatgctac aatggccggt acaaagggtt gcgatgctgt gaggttgagc caatcccaga 1260
aagccggtct cagttcggat tggggtctgc aactcgaccc catgaagtcg gagtcgctag 1320
taatcgcaga tcagcaacgc tgcggtgaat acgttcccgg gccttgtaca caccgcccgt 1380
caagtcacga aagtcggtaa cacccgaagc cggtggccca accccttgtg ggggggagcc 1440
gtcgaaggtg ggactggcga ttgggactaa gtcgtaacaa ggtagccgta ccggaaggtg 1500
cggctggatc acctcctt 1518
Claims (4)
- A kind of 1. Chinese sporangium, it is characterised in that：The bacterial strain is Sinomonas sp.NEAU-HV1, May 18 in 2017 Day is preserved in China typical culture collection center (abbreviation CCTCC), and preservation place is Wuhan City, Hubei Province Wuhan University, preservation Number it is CCTCC NO：M2017246.
- 2. application of the Chinese sporangium in the composition for promoting plant growth is prepared described in claim 1.
- 3. application according to claim 2, it is characterised in that：The composition is to contain Chinese sporangium Sinomonas Sp.NEAU-HV1 microbial inoculum or bio-feritlizer.
- 4. application of the Chinese sporangium in plant growth is promoted described in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711041284.9A CN107586748B (en) | 2017-10-30 | 2017-10-30 | A kind of China's sporangium and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711041284.9A CN107586748B (en) | 2017-10-30 | 2017-10-30 | A kind of China's sporangium and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107586748A true CN107586748A (en) | 2018-01-16 |
| CN107586748B CN107586748B (en) | 2019-11-15 |
Family
ID=61044867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711041284.9A Active CN107586748B (en) | 2017-10-30 | 2017-10-30 | A kind of China's sporangium and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107586748B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108969956A (en) * | 2018-07-27 | 2018-12-11 | 华南农业大学 | A kind of degradation bacteria strains of fungicide kresoxim-methyl and its application |
| CN118147022A (en) * | 2024-05-11 | 2024-06-07 | 海南农乐南繁科技有限公司 | Monomonas darkly B1-1 and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498378A (en) * | 2014-06-23 | 2015-04-08 | 湖北工业大学 | Strain producing aflatoxin B1 degrading enzyme and application thereof |
-
2017
- 2017-10-30 CN CN201711041284.9A patent/CN107586748B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498378A (en) * | 2014-06-23 | 2015-04-08 | 湖北工业大学 | Strain producing aflatoxin B1 degrading enzyme and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| MAHESH ADHIKARI等: "Biological Control of Bacterial Fruit Blotch of Watermelon Pathogen (Acidovorax citrulli) with Rhizosphere Associated Bacteria", 《THE PLANT PATHOLOGY JOURNAL》 * |
| RAJ等: "Molecular characterization of phosphate solubilizing bacteria (PSB) and plant growth promoting rhizobacteria (PGPR) from pristine soil", 《INTERNATIONAL JOURNAL OF INNOVATIVE SCIENCE,ENGINEERING & TECHNOLOGY》 * |
| YU-SHIUAN HUANG等: "Bioprospecting of facultatively oligotrophic bacteria from non-rhizospheric soils", 《APPLIED SOIL ECOLOGY》 * |
| 周育: "中华杆菌科和中华单胞菌属多相分类学研究", 《中国博士学位论文全文数据库 基础科学辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108969956A (en) * | 2018-07-27 | 2018-12-11 | 华南农业大学 | A kind of degradation bacteria strains of fungicide kresoxim-methyl and its application |
| CN108969956B (en) * | 2018-07-27 | 2020-12-29 | 华南农业大学 | Degrading strain of bactericide kresoxim-methyl and application thereof |
| CN118147022A (en) * | 2024-05-11 | 2024-06-07 | 海南农乐南繁科技有限公司 | Monomonas darkly B1-1 and application thereof |
| CN118147022B (en) * | 2024-05-11 | 2024-07-05 | 海南农乐南繁科技有限公司 | Monomonas darkly B1-1 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107586748B (en) | 2019-11-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106591185B (en) | Bacillus amyloliquefaciens plant subspecies and preparation and application of microbial inoculum thereof | |
| CN104877937B (en) | A kind of bacillus amyloliquefaciens bacterial manure and its preparation method and application promoting chili growth | |
| CN106967652B (en) | Rhizobium for promoting growth of common vetch and application of rhizobium | |
| CN103627662B (en) | A kind of Bradyrhizobium sp Arachis and uses thereof | |
| CN104263684B (en) | A kind of product siderophore series bacillus and application thereof | |
| CN103756930B (en) | Peanut rhizosphere biocontrol bacterium, and preparation method and application thereof | |
| CN101497541B (en) | Efficient disease-resistant phosphate solubilizing bacterial manure for tobacco and production method thereof | |
| CN102086444A (en) | Paenibacillus elgii strain and application thereof | |
| CN101948780A (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN101519644B (en) | Sinorhizobium sp. and application thereof | |
| CN103468591B (en) | Salt-tolerant trichoderma pleuroticola strain and application thereof | |
| CN105439657B (en) | A kind of preparation method of the special biologic organic fertilizer of resisting repeated stubbles of strawberry | |
| CN104909846B (en) | A kind of decomposed leavening of compound chicken manure containing bacillus amyloliquefaciens HFJ 7 and its application | |
| CN107586748B (en) | A kind of China's sporangium and its application | |
| CN104593301B (en) | One plant of wall bacillus G1 and its preparation method and application | |
| CN116515673B (en) | Pseudomonas and application of chrysanthemum straw fermentation product thereof in promoting plant growth | |
| CN108841748A (en) | Sinorhizobium nitrogen-fixing bacteria strain H6 and its application | |
| CN102533564B (en) | A kind of screening method of bio-control trichoderma in corn seedling stage root rot period | |
| CN102021129A (en) | Arthrobacterglobiformis CNA9 and application thereof | |
| CN110791459A (en) | Bacillus subtilis for preventing and controlling continuous cropping lily soil-borne blight and application thereof | |
| CN113913344B (en) | Organic material decomposing agent and preparation method thereof | |
| CN108402085A (en) | A kind of biological seedling matrix of prevention tobacco black shank | |
| CN110628674B (en) | Bacillus pumilus with functions of improving acid soil and removing potassium and preparation and application of microbial inoculum thereof | |
| CN108377819A (en) | A method of prevention tobacco black shank | |
| CN115261249B (en) | Rhizobium pisum and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |