CN107586732A - 用于产生丙二酸的重组宿主细胞 - Google Patents

用于产生丙二酸的重组宿主细胞 Download PDF

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Publication number
CN107586732A
CN107586732A CN201710980850.6A CN201710980850A CN107586732A CN 107586732 A CN107586732 A CN 107586732A CN 201710980850 A CN201710980850 A CN 201710980850A CN 107586732 A CN107586732 A CN 107586732A
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China
Prior art keywords
malonic acid
acid
host cell
present
malonyl
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Pending
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CN201710980850.6A
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English (en)
Chinese (zh)
Inventor
J·A·迪特里希
J·L·福特曼
E·J·斯汀
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Lygos Inc
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Lygos Inc
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Publication of CN107586732A publication Critical patent/CN107586732A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/347—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups
    • C07C51/377—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups by splitting-off hydrogen or functional groups; by hydrogenolysis of functional groups
    • C07C51/38—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups by splitting-off hydrogen or functional groups; by hydrogenolysis of functional groups by decarboxylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00—Preparation of carboxylic acid esters
    • C07C67/08—Preparation of carboxylic acid esters by reacting carboxylic acids or symmetrical anhydrides with the hydroxy or O-metal group of organic compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00—Preparation of carboxylic acid esters
    • C07C67/30—Preparation of carboxylic acid esters by modifying the acid moiety of the ester, such modification not being an introduction of an ester group
    • C07C67/31—Preparation of carboxylic acid esters by modifying the acid moiety of the ester, such modification not being an introduction of an ester group by introduction of functional groups containing oxygen only in singly bound form
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02004—3-Hydroxyisobutyryl-CoA hydrolase (3.1.2.4)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201710980850.6A 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞 Pending CN107586732A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261607479P 2012-03-06 2012-03-06
US61/607,479 2012-03-06
US201261619112P 2012-04-02 2012-04-02
US61/619,112 2012-04-02
CN201380023454.4A CN104284974B (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201380023454.4A Division CN104284974B (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞

Publications (1)

Publication Number Publication Date
CN107586732A true CN107586732A (zh) 2018-01-16

Family

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Family Applications (4)

Application Number Title Priority Date Filing Date
CN201710980850.6A Pending CN107586732A (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
CN202111260243.5A Pending CN114395496A (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
CN202410200340.2A Pending CN118086086A (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
CN201380023454.4A Expired - Fee Related CN104284974B (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞

Family Applications After (3)

Application Number Title Priority Date Filing Date
CN202111260243.5A Pending CN114395496A (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
CN202410200340.2A Pending CN118086086A (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞
CN201380023454.4A Expired - Fee Related CN104284974B (zh) 2012-03-06 2013-03-06 用于产生丙二酸的重组宿主细胞

Country Status (8)

Country Link
US (5) US9816114B2 (https=)
EP (2) EP3404094A1 (https=)
CN (4) CN107586732A (https=)
AU (2) AU2013230930B2 (https=)
BR (1) BR112014022117B8 (https=)
ES (1) ES2698500T3 (https=)
MY (1) MY173953A (https=)
WO (1) WO2013134424A1 (https=)

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CN116103172A (zh) * 2021-11-11 2023-05-12 江南大学 一种酿酒酵母通过丙氨酸途径生产丙二酸的方法

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CN107586732A (zh) 2012-03-06 2018-01-16 利戈斯股份有限公司 用于产生丙二酸的重组宿主细胞
EP3161142A4 (en) * 2014-06-26 2018-05-16 Lygos, Inc. Recombinant host cells for the production of malonate
FR3030575A1 (fr) * 2014-12-22 2016-06-24 Inst Nat Des Sciences Appliquees De Toulouse Procede de synthese d'acides gras
US20160230198A1 (en) * 2015-02-10 2016-08-11 GeneSys Consulting, LLC Methylmalonic acid compositions, biological methods for making same, and microorganisms for making same
WO2016130597A1 (en) * 2015-02-10 2016-08-18 GeneSys Consulting, LLC Methylmalonic acid compositions, biological methods for making same, and microorganisms for making same
CA3042854A1 (en) * 2015-11-12 2017-05-18 Lygos, Inc. Recombinant host cells and methods for the anaerobic production of l-aspartate and beta-alanine
KR101828551B1 (ko) * 2016-01-11 2018-02-13 한국과학기술원 말론산 생성능을 가지는 재조합 변이미생물 및 이를 이용한 말론산의 제조방법
US11326191B2 (en) * 2016-11-14 2022-05-10 Lygos, Inc. Process for purification of malonic acid from fermentation broth
WO2019040737A1 (en) * 2017-08-23 2019-02-28 Lygos, Inc. RECOVERING MALONIC ACID AND ITS ESTERS
CN108315343A (zh) * 2018-02-11 2018-07-24 楚雄医药高等专科学校 一种产木犀草素酵母菌株的基因合成方法及菌株和应用
WO2019160862A1 (en) * 2018-02-13 2019-08-22 Lygos, Inc. Method for preparation of diester derivatives of malonic acid
EP3775182A1 (en) * 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides
EP3810786A4 (en) * 2018-06-25 2022-06-08 Lygos, Inc. RECOMBINANT HOST CELLS AND METHODS FOR PRODUCTION OF ASPARTIC ACID AND BETA-ALANINE
BR112021011954A2 (pt) * 2018-12-18 2021-10-13 Alderys Leveduras que produzem semi-aldeído malônico
CN111334444A (zh) * 2018-12-19 2020-06-26 中国科学院微生物研究所 长链二元酸生产菌株及其制备方法和应用
CN110499259B (zh) * 2019-07-22 2021-07-27 浙江工业大学 一种解酯耶氏酵母yw100-1及其应用
CN112410389B (zh) * 2019-08-23 2023-07-18 中国科学院微生物研究所 支链α-酮酸脱氢酶复合体在制备丙二酸单酰辅酶A中的应用
CN119709450A (zh) * 2019-08-30 2025-03-28 利戈斯股份有限公司 用于生产丙二酸的重组宿主细胞
US20230203430A1 (en) * 2019-12-23 2023-06-29 Cargill, Incorporated Fermentation pathway for producing malonic acid
EP4158046A4 (en) * 2020-05-29 2025-07-16 Cargill Inc PROCESS FOR RECOVERING MALONATE FROM FERMENTATION BROTH
US20250269366A1 (en) * 2020-09-21 2025-08-28 Lygos, Inc. Continuous ion exchange and esterification of fermented malonic acid
US20250290082A1 (en) * 2021-05-28 2025-09-18 Cargill, Incorporated Fermentation method for producing malonic acid
EP4355892A1 (en) * 2021-06-17 2024-04-24 Lygos, Inc. Preparing diesters of malonic acid
CN116103173B (zh) * 2021-11-11 2025-06-03 江南大学 一种提高酿酒酵母生产丙二酸的方法
CN116135981B (zh) * 2021-11-18 2024-07-12 中国科学院天津工业生物技术研究所 一种合成丙二酸的方法及应用
CN114736841B (zh) * 2022-04-02 2023-12-19 万华化学集团股份有限公司 一种重组大肠杆菌及其制备方法与应用
WO2024054828A2 (en) * 2022-09-06 2024-03-14 Lygos, Inc. Recombinant host cells for the production of malonate
CN120554239A (zh) * 2022-09-09 2025-08-29 厦门赛诺邦格生物科技股份有限公司 一种碳核阳离子脂质
WO2025038447A1 (en) * 2023-08-11 2025-02-20 Ginkgo Bioworks, Inc. Engineered cells for production of malonate
CN117757766B (zh) * 2024-02-20 2024-05-14 中国科学院天津工业生物技术研究所 醛还原酶突变体及其在合成d-1,2,4-丁三醇中的应用
CN119391682A (zh) * 2025-01-03 2025-02-07 浙江科技大学 一种谷氨酸脱羧酶多位点组合突变体及其应用

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Publication number Priority date Publication date Assignee Title
CN116103172A (zh) * 2021-11-11 2023-05-12 江南大学 一种酿酒酵母通过丙氨酸途径生产丙二酸的方法
CN116103172B (zh) * 2021-11-11 2025-06-03 江南大学 一种酿酒酵母通过丙氨酸途径生产丙二酸的方法

Also Published As

Publication number Publication date
EP2823032A1 (en) 2015-01-14
US20170362614A1 (en) 2017-12-21
BR112014022117B1 (pt) 2022-02-15
WO2013134424A1 (en) 2013-09-12
EP3404094A1 (en) 2018-11-21
CN114395496A (zh) 2022-04-26
EP2823032B1 (en) 2018-08-22
BR112014022117B8 (pt) 2023-01-10
US20240191216A1 (en) 2024-06-13
EP2823032A4 (en) 2016-03-02
ES2698500T3 (es) 2019-02-05
BR112014022117A2 (https=) 2017-06-20
CN104284974A (zh) 2015-01-14
US20220119783A1 (en) 2022-04-21
US9816114B2 (en) 2017-11-14
US11248215B2 (en) 2022-02-15
CN118086086A (zh) 2024-05-28
US20160177345A1 (en) 2016-06-23
US10472653B2 (en) 2019-11-12
MY173953A (en) 2020-02-28
US20200071733A1 (en) 2020-03-05
AU2019200158A1 (en) 2019-01-31
CN104284974B (zh) 2018-06-26
AU2013230930A1 (en) 2014-10-23
AU2013230930B2 (en) 2018-10-11

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